Visualization device used in biological cell freezing process

Abstract

The utility model relates to the technical field of biology, and discloses a visualization device used in a biological cell freezing process, which comprises a freezing cooling mechanism and an observation mechanism, the freezing cooling mechanism is positioned at the bottom of the observation mechanism, the freezing cooling mechanism comprises a metal woven belt, the surface of the metal woven belt is fixedly connected with a constant low-temperature copper table, and the constant low-temperature copper table is fixedly connected with the observation mechanism. A lifting sliding table is fixedly connected to the bottom of the constant low-temperature copper table, and a deep low-temperature liquid hose externally provided with heat preservation cotton is arranged on the surface of the constant low-temperature copper table. According to the utility model, the metal woven belt is arranged, so that biological cells can be quickly frozen; and the device has the capability of observing video recording conditions in the whole process and high-frequency real-time temperature measurement, and can be used for observing and recording a liquid state, an instant glass state or a crystallization conversion detail process of a biological cell freezing process in a few seconds in real time. The method plays an important role in the field of research on cryopreservation of deep hypothermia biological cells.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a visualization device used in the freezing process of biological cells. Background Art

[0002] Rapid cell freezing is one of the research hotspots in the biological field, and vitrification is a rapid freezing technology. In order to enable the sample to be in contact with the low-temperature cold surface but to cool down quickly, and to visualize the rapid freezing process, a freezing and cooling module based on the conduction of cold energy by metal braided belts in a microscope video system for visualizing the freezing process of biological cells was designed and developed. It has important value.

[0003] The papers and patents on microscopic imaging systems for observing the rapid freezing and thawing process of cell vitrification that have been published so far have conduction cooling and freezing and cooling modules that are different from this device. For example, Chinese patent CN 110057821A discloses a cryogenic microscopic imaging system for observing the rapid freezing and thawing process of human gametes, especially for human gametes. Its conduction cooling and freezing and cooling module moves the gamete visual environment chamber downward and makes its surface in close contact with the liquid nitrogen heat sink cavity. When the movement stops, the liquid nitrogen heat sink cavity quickly transfers the cold to the sample in the gamete visual environment chamber. For example, Chinese patent CN109997841 B discloses a human gamete rapid freezing and thawing visualization environment chamber that maintains a normal pressure air atmosphere. Its conduction cooling and freezing and cooling module moves the overall position of the environment chamber to just above the low-temperature cold surface, and then moves downward until the high thermal conductivity bottom plate at the bottom of the environment chamber body is in close contact with the low-temperature cold surface for rapid cooling. For example, Chinese patent CN 114544317 A discloses a freeze-thaw instrument for cell detection, in which the conduction cooling module is to install the refrigerator inside a thermos bottle, and the cooling end of the refrigerator is connected to a copper rod. The refrigerator is used to lower the temperature of the copper rod, and the copper rod transfers the temperature to the sample chamber, thereby lowering the temperature of the sample chamber and achieving the purpose of freezing. Utility Model Content

[0004] In order to solve the above technical problems, the utility model provides a visualization device used in the freezing process of biological cells.

[0005] The utility model is implemented by the following technical scheme: a visualization device for biological cell freezing process, comprising a freezing and cooling mechanism and an observation mechanism, wherein the freezing and cooling mechanism is located at the bottom of the observation mechanism;

[0006] The freezing and cooling mechanism comprises a metal braided belt, a constant-temperature copper platform is fixedly connected to the surface of the metal braided belt, a lifting slide is fixedly connected to the bottom of the constant-temperature copper platform, a deep-temperature liquid soft pipe with external insulation cotton is arranged on the surface of the constant-temperature copper platform, a deep-temperature liquid tank is fixedly connected to the surface of the deep-temperature liquid soft pipe with external insulation cotton, a vacuum pump interface is arranged on the surface of the constant-temperature copper platform, a metal braided belt cold end fixing bracket is fixedly connected to the surface of the constant-temperature copper platform, a biological cell sample placement cold platform is fixedly connected to the surface of the metal braided belt, a thermal couple is arranged on the surface of the metal braided belt cold end fixing bracket, a bracket platform is fixedly connected to the surface of the thermocouple, a cover plate with an observation window is arranged on the surface of the bracket platform, quartz glass is arranged inside the cover plate with the observation window, a groove is arranged inside the biological cell sample placement cold platform, and an O-type high-insulation sealing ring is arranged inside the groove.

[0007] Through the above technical solution, the lifting slide is raised in a controlled manner, and the constant low temperature copper platform is lifted up to be in close contact with the cold end of the metal braided belt to conduct cold. The cold energy is conducted to the biological cell sample on the slide in the groove of the biological cell sample placement cold platform and the temperature is quickly reduced. This lifting can prevent the sample from being affected by low temperature in the standby state, and once cooling is required, it can be quickly achieved. The cold energy is conducted by the metal braided belt. The metal braided belt and the biological cell sample placement cold platform are made of pure copper or pure silver. The cooling efficiency is extremely high and the speed is extremely fast, which can quickly cool the biological cell sample.

[0008] As a further improvement of the above solution, one end of the metal braided belt is fixedly connected to the constant low temperature copper table, and the other end of the metal braided belt is fixedly connected to the metal braided belt cold end fixing bracket.

[0009] As a further improvement of the above scheme, the metal braided belt (1) is made of pure copper or pure silver, and is wrapped with heat-insulating cotton. The cold end is made of pure copper or pure silver for heat insulation, and the other end has an optical path through hole in the center, which is also made of pure copper or pure silver.

[0010] Through the above technical solution, cold energy is conducted through the metal braided belt. The metal braided belt and the cold platform for placing the biological cell sample are made of pure copper or pure silver. The cooling efficiency is extremely high and the speed is extremely fast, which can quickly cool down the biological cell sample.

[0011] As a further improvement of the above solution, the cryogenic liquid soft pipe with added thermal insulation cotton is connected to and penetrates into the bottom of the cryogenic liquid tank.

[0012] As a further improvement of the above scheme, the observation mechanism includes a biological microscope, a video recorder is fixedly connected to the top of the biological microscope, a main bracket is fixedly connected to the top of the video recorder, and a base plate is fixedly connected to the bottom of the main bracket.

[0013] As a further improvement of the above scheme, the biological microscope is arranged above the cover plate with the observation window, and the lifting slide and the deep-cold liquid soft pipe with external insulation cotton are arranged above the bottom plate.

[0014] Compared with the prior art, the beneficial effects of the present invention are:

[0015] The utility model arranges a metal braided belt to conduct cold energy. The metal braided belt and the cold platform for placing the biological cell sample are both made of pure copper or pure silver. The cold conduction efficiency is extremely high and the speed is extremely fast, so the biological cell sample can be quickly cooled.

[0016] The utility model sets a lifting slide, and the lifting slide is raised in a controlled manner, so that the constant low temperature copper platform is lifted up and closely contacts the cold end of the metal braided belt to conduct cold, and the cold energy is conducted to the biological cell sample on the slide in the groove of the cold platform where the biological cell sample is placed, and the temperature is quickly reduced. This lifting can prevent the sample from being affected by the low temperature in the standby state, and once the temperature needs to be reduced, the temperature can be quickly reduced.

[0017] The utility model starts the vacuum pump in a controlled manner, and the deep cryogenic liquid flows in the pipeline, avoiding the observation light path, thereby preventing fogging from affecting the observation. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0019] Figure 2 This is a schematic diagram of the internal structure of the cover plate with an observation window as a whole according to the utility model;

[0020] Figure 3 The utility model is a schematic diagram of the structure of a microscope video recording system for visualizing the whole biological cell freezing process.

[0021] Description of main symbols:

[0022] 1. Freezing and cooling mechanism; 101. Metal braided belt; 102. Constant-temperature copper table; 103. Lifting slide; 104. Cryogenic liquid tank; 105. Cryogenic liquid soft pipe with external insulation cotton; 106. Vacuum pump interface; 107. Metal braided belt cold end fixing bracket; 108. Cold platform for placing biological cell samples; 109. Thermocouple; 110. Bracket platform; 111. Cover with observation window; 112. Quartz glass; 113. Groove; 114. O-type high-insulation sealing ring; 2. Observation mechanism; 201. Biological microscope; 202. Video recorder; 203. Main bracket; 204. Bottom plate. DETAILED DESCRIPTION

[0023] The present invention is further described below in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0024] Embodiment 1:

[0025] Please combine Figure 1-3 , a visualization device for biological cell freezing process in this embodiment includes a freezing and cooling mechanism 1 and an observation mechanism 2, wherein the freezing and cooling mechanism 1 is located at the bottom of the observation mechanism 2;

[0026] The freezing and cooling mechanism 1 includes a metal braided belt 101, a constant temperature copper platform 102 is fixedly connected to the surface of the metal braided belt 101, a lifting slide 103 is fixedly connected to the bottom of the constant temperature copper platform 102, a deep low temperature liquid soft pipe 105 with external insulation cotton is arranged on the surface of the constant temperature copper platform 102, a deep low temperature liquid soft pipe 105 with external insulation cotton is fixedly connected to the surface of the deep low temperature liquid tank 104, a vacuum pump interface 106 is arranged on the surface of the constant temperature copper platform 102, and a metal braided belt cold end fixedly connected to the surface of the constant temperature copper platform 102 The surface of the bracket 107 and the metal braided belt 101 is fixedly connected with a cold platform 108 for placing biological cell samples, a thermocouple 109 is arranged on the surface of the cold end fixed bracket 107 of the metal braided belt, a bracket platform 110 is fixedly connected to the surface of the thermocouple 109, a cover plate 111 with an observation window is arranged on the surface of the bracket platform 110, a quartz glass 112 is arranged inside the cover plate 111 with the observation window, a groove 113 is arranged inside the cold platform 108 for placing biological cell samples, and an O-type high insulation sealing ring 114 is arranged inside the groove 113.

[0027] One end of the metal braided belt 101 is fixedly connected to the constant low temperature copper platform 102 , and the other end of the metal braided belt 101 is fixedly connected to the cold end fixing bracket of the metal braided belt 101 .

[0028] The metal braided belt 101 is made of pure copper or pure silver, and is wrapped with heat-insulating cotton. The cold end is made of pure copper or pure silver for heat insulation, and the other end is provided with an optical path through hole in the center, which is also made of pure copper or pure silver.

[0029] The cryogenic liquid soft pipe 105 with thermal insulation cotton is connected to and penetrates into the bottom of the cryogenic liquid tank 104 .

[0030] The observation mechanism 2 includes a biological microscope 201 , a video recorder 202 is fixedly connected to the top of the biological microscope 201 , a main bracket 203 is fixedly connected to the top of the video recorder 202 , and a bottom plate 204 is fixedly connected to the bottom of the main bracket 203 .

[0031] The biological microscope 201 is arranged above the cover plate 111 with the observation window, and the lifting slide 103 and the deep-cryogenic liquid soft pipe 105 with external insulation cotton are arranged above the bottom plate.

[0032] The implementation principle of a visualization device for biological cell freezing process in the embodiment of the present application is as follows: first, the vacuum pump is controlled to be started, and the cryogenic liquid in the cryogenic liquid tank flows into the constant low temperature copper platform along the cryogenic liquid soft pipe with external insulation cotton to evaporate and cool down to the cryogenic liquid vaporization temperature, then the cover with the observation window is opened, and the slide glass with the biological cell sample is placed in the groove of the biological cell sample placement cold platform; the cover with the observation window is covered, and when the preparation work is completed, the lifting slide is controlled to rise, the constant low temperature copper platform is lifted up and closely contacts the cold end of the metal braided belt to conduct cooling, and the cold amount is conducted to the biological cell sample placement cold platform, and the biological cell sample on the slide glass in the groove is quickly cooled; the lifting and close contact cooling time is calculated and controlled according to the comparison between the temperature measured by the thermocouple and the set target temperature, and then the liquid state, instantaneous glass state, or crystallization conversion details of the biological cell freezing process within a few seconds can be observed and recorded in real time through the observation mechanism.

[0033] The above-mentioned implementation modes are only preferred implementation modes of the present invention, and cannot be used to limit the protection scope of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A visualization device for biological cell freezing process, characterized in that: It comprises a freezing and cooling mechanism (1) and an observation mechanism (2), wherein the freezing and cooling mechanism (1) is located at the bottom of the observation mechanism (2); The freezing and cooling mechanism (1) comprises a metal braided belt (101), a constant low temperature copper platform (102) is fixedly connected to the surface of the metal braided belt (101), a lifting slide (103) is fixedly connected to the bottom of the constant low temperature copper platform (102), a deep low temperature liquid soft pipe (105) with external insulation cotton is arranged on the surface of the constant low temperature copper platform (102), a deep low temperature liquid soft pipe (105) with external insulation cotton is fixedly connected to the surface of the deep low temperature liquid soft pipe (105) with external insulation cotton is fixedly connected to the deep low temperature liquid tank (104), a vacuum pump interface (106) is arranged on the surface of the constant low temperature copper platform (102), and a metal braided belt cold end fixing bracket is fixedly connected to the surface of the constant low temperature copper platform (102). (107), a biological cell sample placement cold platform (108) is fixedly connected to the surface of the metal braided belt (101), a thermocouple (109) is arranged on the surface of the cold end fixing bracket (107) of the metal braided belt, a bracket platform (110) is fixedly connected to the surface of the thermocouple (109), a cover plate (111) with an observation window is arranged on the surface of the bracket platform (110), a quartz glass (112) is arranged inside the cover plate (111) with the observation window, a groove (113) is arranged inside the biological cell sample placement cold platform (108), and an O-type high-insulation sealing ring (114) is arranged inside the groove (113).

2. A visualization device for biological cell freezing process according to claim 1, characterized in that: One end of the metal braided belt (101) is fixedly connected to the constant low temperature copper platform (102), and the other end of the metal braided belt (101) is fixedly connected to a cold end fixing bracket of the metal braided belt (101).

3. A visualization device for biological cell freezing process according to claim 1, characterized in that: The metal braided belt (101) is made of pure copper or pure silver and is wrapped with heat-insulating cotton. The cold end is made of heat-insulating pure copper or pure silver, and the center of the other end is provided with an optical path through hole, which is also made of pure copper or pure silver.

4. A visualization device for biological cell freezing process as claimed in claim 1, characterized in that: The cryogenic liquid soft pipe (105) with external heat insulation cotton is connected to and penetrates into the bottom of the cryogenic liquid tank (104).

5. A visualization device for biological cell freezing process as claimed in claim 1, characterized in that: The observation mechanism (2) comprises a biological microscope (201), the top of the biological microscope (201) is fixedly connected to a video recorder (202), the top of the video recorder (202) is fixedly connected to a main support (203), and the bottom of the main support (203) is fixedly connected to a base plate (204).

6. A visualization device for biological cell freezing process as claimed in claim 5, characterized in that: The biological microscope (201) is arranged above a cover plate (111) with an observation window, and the lifting slide (103) and the cryogenic liquid soft pipe (105) with external heat insulation cotton are arranged above the bottom plate.

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