Anti-pollution cell culture medium bottle

Abstract

The utility model discloses an anti-pollution cell culture medium bottle which comprises a bottle body and a bottle cap covering a bottle opening of the bottle body, a bottle shoulder of the bottle body is provided with a groove and a cover body covering the groove, the side wall of the groove or the cover body is provided with a gas filling connector, the bottom face of the groove is provided with a plurality of liquid filling openings, and the liquid filling openings are communicated with the groove. The liquid adding openings are connected with first covers in an openable and closable mode, openings are formed in the positions, corresponding to the liquid adding openings, of the cover body, and the openings are provided with second covers capable of being opened and closed. Wherein, in a plan view projection, the first cover is contained in the opening. The problem that an existing culture medium bottle is prone to infection is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture equipment and relates to an anti-pollution cell culture medium bottle. Background Art

[0002] At present, cell culture technology can transform a cell into a simple single cell or a few differentiated multi-cells through mass culture. This is an indispensable part of cloning technology, and cell culture itself is a clone of cells. A large number of cells or their metabolites can be obtained through cell culture. Because biological products are all derived from cells, it can be said that cell culture technology is the most core and basic technology in biotechnology.

[0003] During the process of culturing cells in culture flasks, they need to be regularly given a variety of nutrients and drugs. The existing technology can only unscrew the bottle cap or insert a straw to add nutrients, which can easily lead to infection from external bacteria, thereby reducing the survival rate of cells. Utility Model Content

[0004] The utility model aims to provide a contamination-resistant cell culture medium bottle, thereby solving the problem that the existing culture medium bottle is prone to infection.

[0005] The technical solution adopted by the utility model is as follows:

[0006] A contamination-proof cell culture medium bottle comprises a bottle body and a bottle cap covered on the bottle mouth of the bottle body, the bottle shoulder of the bottle body is provided with a groove and a cover body covered on the groove, a gas filling joint is provided on the side wall of the groove or the cover body, a plurality of liquid filling ports are provided on the bottom surface of the groove, the liquid filling ports are connected to a first cover in an openable and closable manner, an opening is provided on the cover body corresponding to each of the liquid filling ports, and the opening is provided with a second cover that can be opened and closed; wherein, in a top view projection, the first cover is contained in the opening.

[0007] Furthermore, it also includes a compressed inert gas tank, which is connected to the gas filling connector and a switch valve is provided between the two.

[0008] Furthermore, an ultraviolet lamp is built into the groove.

[0009] Furthermore, the cover body is rotatably connected to the groove.

[0010] Furthermore, the cover body is buckled with the groove.

[0011] Furthermore, the cover body and the bottle shoulder are integrally arranged.

[0012] Furthermore, the first cover is made of plastic and is buckled into the liquid filling port.

[0013] Furthermore, the second cover is connected to the cover body in a horizontally rotatable manner.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] The cell culture medium bottle of the utility model comprises a bottle body and a bottle cap which covers the bottle mouth of the bottle body. When adding nutrients into the culture medium bottle, sterilized inert gas is connected through a gas filling joint to make the groove present a positive pressure state and fill the groove with inert gas. Then, the corresponding second cap is opened to make the original gas in the groove flow out. Then, the first cap is opened and nutrients are added from the liquid adding port through a nutrient tube. The plurality of liquid adding ports can each correspond to a nutrient. When each nutrient is added, the corresponding first cap and second cap are opened separately. In the process of adding nutrients, the groove is always kept at a positive pressure and filled with sterilized inert gas, thereby solving the problem that the existing culture medium bottle is prone to infection. After the nutrients are added, the substances in the cell culture medium bottle can be stirred through the liquid adding port, which is beneficial to the culture of cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present utility model and should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work, including:

[0017] Figure 1 This is a schematic structural diagram of an anti-pollution cell culture medium bottle according to an embodiment of the utility model;

[0018] Figure 2 for Figure 1 Enlarged view of point A in the middle.

[0019] Markings in the figure:

[0020] 10-bottle body; 11-bottle mouth; 12-groove; 121-liquid filling port; 122-first cover; 123-ultraviolet lamp; 13-cover body; 131-opening; 132-second cover; 133-gas filling joint;

[0021] 20-Bottle caps. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the utility model and are not used to limit the utility model, that is, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. The components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0023] Therefore, the following detailed description of the embodiments of the utility model provided in the accompanying drawings is not intended to limit the scope of the utility model claimed for protection, but merely represents selected embodiments of the utility model. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the utility model.

[0024] It should be noted that relational terms such as "first" and "second" are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0025] As described in the background technology, cell culture technology can transform a cell into a simple single cell or a few differentiated multi-cells through mass culture. This is an indispensable part of cloning technology, and cell culture itself is a clone of cells. A large number of cells or their metabolites are obtained through cell culture. Because biological products are all derived from cells, it can be said that cell culture technology is the most core and basic technology in biotechnology.

[0026] During the process of culturing cells in culture flasks, they need to be regularly given a variety of nutrients and drugs. The existing technology can only unscrew the bottle cap or insert a straw to add nutrients, which can easily lead to infection from external bacteria, thereby reducing the survival rate of cells.

[0027] Based on this, the inventors have created an anti-contamination cell culture medium bottle of the present application to solve the above technical problems.

[0028] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0029] Example

[0030] See also Figure 1 , Figure 2 , a contamination-proof cell culture medium bottle, comprising a bottle body 10 and a bottle cap covering the bottle mouth 11 of the bottle body 10. The bottle shoulder of the bottle body 10 is provided with a groove 12 and a cover body 13 covering the groove 12. Here, the bottle shoulder refers to the portion of the bottle body 10 below the bottle mouth 11 that is in a shoulder state. For example, the groove 12 can be square or circular, for example, the cover body 13 is rotatably connected to the groove 12 (for example, horizontal rotation connection or vertical rotation connection), for example, the cover body 13 is buckled with the groove 12, and such a configuration is easy to open and close. For example, the cover body 13 is integrally arranged with the bottle shoulder, which is convenient for manufacturing. A gas filling joint 133 is arranged on the side wall of the groove 12 or the cover body 13. Here, the gas filling joint 133 is used to pass a sterilized gas with chemical stability, such as nitrogen, argon, etc., but the utility model is not limited thereto. The bottom surface of the groove 12 is provided with a plurality of liquid adding ports 121, and the liquid adding ports 121 are connected to a first cover 122 in an openable and closable manner. For example, the first cover 122 is a plastic or rubber part, has good sealing performance, and is buckled into the liquid adding port 121, and the cover is stable. The cover body 13 is provided with an opening 131 corresponding to each of the liquid adding ports 121, and the opening 131 is provided with a second cover 132 that can be opened and closed. For example, the second cover 132 can be connected to the cover body 13 in a horizontally rotatable manner.

[0031] In the top view, the first cover 122 is contained in the opening 131 , that is, the first cover 122 can be opened and material can be added through the opening 131 .

[0032] The cell culture medium bottle of the utility model comprises a bottle body 10 and a bottle cap covered on a bottle mouth 11 of the bottle body 10. When adding nutrients to the culture medium bottle, sterilized inert gas is connected through a gas adding joint 133 to make the groove 12 present a positive pressure state and fill the groove 12 with inert gas. Then, the corresponding second cap 132 is opened to make the original gas in the groove 12 gush out. Then, the first cap 122 is opened and nutrients are added from a liquid adding port 121 through a nutrient tube. The plurality of liquid adding ports 121 may each correspond to a nutrient. When each nutrient is added, the corresponding first cap 122 and second cap 132 are opened separately. In the process of adding nutrients, the groove 12 is always kept at a positive pressure and filled with sterilized inert gas, thereby solving the problem that the existing culture medium bottle is prone to infection. After adding nutrients, the substances in the cell culture medium bottle can also be stirred through the liquid adding port 121, which is beneficial to the culture of cells.

[0033] In another embodiment, a compressed inert gas tank is further included, and the compressed inert gas tank is connected to the gas filling connector 133 and a switch valve is provided between the two. In this way, the compressed inert gas tank can provide a continuous positive pressure for the groove 12, thereby preventing bacteria in the outside air from entering the cell culture medium bottle and preventing the culture from being contaminated.

[0034] In another embodiment, the groove 12 is built with an ultraviolet lamp 123, so that the ultraviolet lamp 123 can further sterilize and prevent contamination.

[0035] The above description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions and improvements made by any technician familiar with the field within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A contamination-resistant cell culture medium bottle, characterized in that: It includes a bottle body and a bottle cap covered on the bottle mouth of the bottle body, the bottle shoulder of the bottle body is provided with a groove and the cover body is covered on the groove, the side wall of the groove or the cover body is provided with a gas filling joint, the bottom surface of the groove is provided with a plurality of liquid filling ports, the liquid filling ports are openably connected to a first cover, the cover body is provided with an opening corresponding to each of the liquid filling ports, and the opening is provided with an openable and closable second cover; wherein, in a top view projection, the first cover is contained in the opening.

2. The anti-contamination cell culture medium bottle according to claim 1, characterized in that: It also includes a compressed inert gas tank, which is communicated with the gas filling connector and a switch valve is arranged between the two.

3. The anti-contamination cell culture medium bottle according to claim 2, characterized in that: An ultraviolet lamp is built in the groove.

4. The anti-contamination cell culture medium bottle according to claim 1, characterized in that: The cover body is rotatably connected to the groove.

5. The anti-contamination cell culture medium bottle according to claim 4, characterized in that: The cover body is buckled with the groove.

6. The anti-contamination cell culture medium bottle according to claim 1, characterized in that: The cover body and the bottle shoulder are integrally arranged.

7. The anti-contamination cell culture medium bottle according to claim 1, characterized in that: The first cover is a plastic part and is buckled in the liquid filling port.

8. The anti-contamination cell culture medium bottle according to claim 7, characterized in that: The second cover is connected to the cover body in a horizontally rotatable manner.

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