Variable-volume chick embryo ex-shell incubation device for experiment

Abstract

The utility model provides a variable-volume chick embryo ex-shell incubation device for an experiment, and relates to the technical field of medical experiment devices. The out-of-shell incubation device provided by the utility model comprises a base, the movable structure is arranged on the base, and the movable structure can move in the axis direction of the base; the hatching structure is arranged between the base and the movable structure, one end of the hatching structure is connected with the base, the other end of the hatching structure is connected with the movable structure, and a hatching space is formed in the hatching structure. The movable structure moves in the axis direction of the base so as to adjust the volume of the incubation space in the incubation structure, the volume of the incubation space can be adaptively adjusted in the development process of chick embryos, enough space is provided for egg yolk deformation and CAM development in all stages of chick embryo development, it is ensured that CAM is flatly laid on the egg yolk for growth, and the incubation efficiency is improved. The success rate of chick embryo culture is improved, and subsequent experimental operation is facilitated; and the volume-adjustable incubation space improves the universality of the out-shell incubation device.

Description

Technical Field

[0001] The present application relates to the technical field of medical experimental devices, and in particular to a variable volume chicken embryo incubation device for experiments. Background Art

[0002] The chicken embryo begins to form a vascularized chicken embryo chorioallantoic membrane structure on the third day of development. In the first two weeks of development, the chicken embryo's immune system has not yet matured and can hardly produce an immune response. Therefore, heterologous tissues or cells can be transplanted into the chicken embryo chorioallantoic membrane for growth and experimental research. Compared with traditional nude mouse tumor transplantation experiments, this method has advantages such as fast tumor growth and has broad experimental and clinical application prospects.

[0003] Existing in vitro incubation methods often adopt the chicken embryo to be transferred to a flat-bottomed culture dish. Under natural conditions, the chicken embryo in the unhatched and early hatching stage is always located above the yolk. Even if the egg rotates, the round-shaped yolk (yolk sac) suspended in the egg white will rotate spontaneously to ensure that the embryo surface is located above. When the shell is cultivated outside by the flat-bottomed culture dish incubation method, the embryo surface will touch the bottom, and even only part of the wall will greatly affect the survival rate of the embryo. The flat-bottomed culture dish incubation method, after the chicken embryo is transplanted therein, due to the large deformation of the yolk, the tension on the yolk surface rises suddenly, which is very likely to cause the damage of the yolk. Moreover, the chicken embryo has higher requirements for temperature and humidity in the process of development. The flat-bottomed culture dish incubation method often causes the embryo to lose moisture because the embryo exceeds the egg white liquid level, and the humidity is difficult to control, so the success rate is very low.

[0004] Secondly, the utility model patent with application number 201120266003.1 also discloses a chicken embryo culture dish. This patent is designed with a space specifically for accommodating egg yolks. After the embryo is transplanted into it, it can basically simulate its suspended state in the eggshell, giving the fetus space to rotate spontaneously. This avoids malposition of the fetus and direct exposure of the embryo, which will dry up and die. However, during the development of the chicken embryo, the shape of the egg yolk is not static, but it keeps getting bigger during the development process, losing its original round-like shape and size. Secondly, the design of this patent obviously ignores the differences between individuals, which affects its universality. Furthermore, the patent designs a "T"-shaped incubation space, intending to provide space for the development of the chicken embryo chorioallantoic membrane to facilitate subsequent experimental operations. The formation and development of CAM (chicken embryo chorioallantoic membrane) are always close to the embryo and the yolk. Under this incubation device, out-of-shell incubation is carried out. It is possible that the CAM is not flat on the platform of the "T"-shaped space as expected, but is close to the yolk and, under the influence of gravity, grows all over the side walls of the space that accommodates the yolk. Utility Model Content

[0005] Based on this, the present application provides an experimental variable volume chicken embryo shell incubation device, which can change the volume of the incubation space and improve the success rate of chicken embryo culture.

[0006] An experimental variable volume chicken embryo shell incubation device, comprising:

[0007] Pedestal;

[0008] A movable structure is disposed on the base, and the movable structure can move along the axis direction of the base;

[0009] The incubation structure is arranged between the base and the movable structure. One end of the incubation structure is connected to the base, and the other end is connected to the movable structure. The interior of the incubation structure forms an incubation space.

[0010] Optionally, a cross-sectional area of ​​the incubation space along the horizontal direction increases in a direction from the base to the movable structure.

[0011] Optionally, the hatching structure comprises a resilient material.

[0012] Optionally, the base is provided with an adjusting member for driving the movable structure to move along the axial direction of the base.

[0013] Optionally, the adjusting member is rotatably disposed on the base and is threadedly connected to the base, and one end of the adjusting member can contact the movable structure, thereby driving the movable structure to move along the axial direction of the base.

[0014] Optionally, the base is provided with a guide block, and the movable structure is provided with a guide groove matched with the guide block, and the guide block and the guide groove are used to limit the rotation of the movable structure.

[0015] Optionally, a buckle is further included, which is arranged at the center of the hatching structure and connected to the base, and is used to connect the center of the hatching structure to the base.

[0016] Optionally, the buckle is a “T”-shaped buckle.

[0017] Optionally, a fixing ring is further included, which is arranged on the movable structure and is used to connect the edge of the hatching structure with the movable structure.

[0018] Optionally, the base and the movable structure are both cylindrical, and the movable structure is nested outside the base.

[0019] The present application discloses an experimental variable volume chicken embryo shell incubation device, which adjusts the volume of the hatching space inside the hatching structure by moving the movable structure along the axial direction of the base. The hatching space volume can be adaptively adjusted during the development of the chicken embryo, so as to provide sufficient space for yolk deformation and CAM development at various stages of chicken embryo development, ensure that the CAM is laid flat on the yolk growth, improve the success rate of chicken embryo culture, and facilitate subsequent experimental operations; secondly, the hatching space with adjustable volume can also be applied to eggs of different sizes, thereby improving the versatility of use. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without exceeding the scope of protection required by the present application.

[0021] Figure 1 This is a schematic assembly diagram of the structure of an experimental variable volume chicken embryo shell incubation device provided by the present application;

[0022] Figure 2 It is a cross-sectional view along AA of an experimental variable volume chicken embryo shell incubation device provided by the present application;

[0023] Figure 3 It is a side exploded view of an experimental variable volume chicken embryo shell incubation device provided by the present application;

[0024] Figure 4 It is a three-dimensional exploded view of an experimental variable volume chicken embryo shell incubation device provided by the present application;

[0025] Figure 5 It is a schematic diagram of the structure of a fixed ring in an experimental variable volume chicken embryo shell incubation device provided by the present application.

[0026] Explanation of the reference numerals: 1. base; 2. movable structure; 3. hatching structure; 4. adjusting member; 5. guide block; 6. guide groove; 7. buckle; 8. fixing ring; 81. gap adjustment part. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present application.

[0028] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present application, "multiple" means two or more, unless otherwise clearly and specifically defined.

[0029] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0030] In the present application, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0031] refer to Figure 1 and Figure 2 An experimental variable volume chicken embryo shell incubation device comprises a base 1, a movable structure 2 and an incubation structure 3; the movable structure 2 is arranged on the base 1, and the movable structure 2 can move along the axial direction of the base 1; the incubation structure 3 is arranged between the base 1 and the movable structure 2, one end of the incubation structure 3 is connected to the base 1, and the other end is connected to the movable structure 2, and the interior of the incubation structure 3 forms an incubation space.

[0032] During the movement of the movable structure 2 along the axis direction of the base 1, the distance between the movable structure 2 and the base 1 changes. Since the two ends of the hatching structure 3 are connected to the base 1 and the movable structure 2 respectively, the volume of the hatching space inside the hatching structure 3 also changes accordingly with the change of the distance between the movable structure 2 and the base 1; when the distance between the movable structure 2 and the base 1 increases, the volume of the hatching space increases, and when the distance between the movable structure 2 and the base 1 decreases, the volume of the hatching space decreases. By moving the movable structure 2 along the axis direction of the base 1 to adjust the volume of the hatching space inside the hatching structure 3, the volume of the hatching space can be adaptively adjusted during the development of the chicken embryo, so as to provide enough space for the yolk deformation and CAM development at various stages of the chicken embryo development, ensure that the CAM is flattened on the yolk growth, improve the success rate of the chicken embryo culture, and facilitate the subsequent experimental operation; secondly, the hatching space with adjustable volume can also be applied to eggs of different sizes, which improves the versatility of the variable volume chicken embryo shell incubation device for experiments.

[0033] refer to Figure 1 and Figure 2 As an optional implementation, the movable structure 2, the hatching structure 3 and the base 1 are arranged in sequence from top to bottom, and the cross-sectional area of ​​the hatching space along the horizontal direction increases in the direction from the base 1 to the movable structure 2.

[0034] The horizontal cross-sectional area of ​​the incubation space increases from bottom to top. When the distance between the movable structure 2 and the base 1 is adjusted to change the size of the incubation space volume, the liquid level of the chicken embryo yolk and egg white liquid in the incubation space also changes accordingly. When the liquid level moves up relative to the incubation space, the plane area of ​​the liquid surface increases. Conversely, when the liquid level moves down relative to the incubation space, the plane area of ​​the liquid surface decreases. Therefore, by adjusting the size of the incubation space volume, sufficient development space can be given to the CAM, the yolk deformation rate can be controlled, the yolk surface tension can be reduced, and the breakage rate can be reduced.

[0035] For example, the horizontal cross-sectional area of ​​the incubation space increases linearly from bottom to top. The incubation space with a gradually increasing horizontal cross-sectional area from bottom to top allows the yolk to be suspended in the egg white and maintain its original circular shape, which is conducive to continuing the original surface tension and reducing the probability of yolk breakage. When the yolk is suspended, it can spontaneously rotate the embryo surface to the top, thereby improving the survival rate of the embryo. Secondly, the upper horizontal cross-sectional area of ​​the incubation space of the present application increases linearly and continuously without any faults, making it easier to provide sufficient space to assist the CAM in flattening and development while avoiding yolk breakage.

[0036] As an optional embodiment, the hatching structure 3 includes an elastic material, such as a rubber film.

[0037] The hatching structure 3 made of elastic material has good elasticity, ductility and continuity; it can softly hold the chicken embryo. At the same time, when adjusting the volume of the hatching space, the hatching structure 3 can adaptively and continuously stretch or shrink and restore to adapt to the changes in the hatching space, giving the CAM sufficient development space, thereby avoiding the CAM being limited by the growth space and the influence of gravity.

[0038] As an optional implementation, the base 1 is provided with an adjusting member 4 for driving the movable structure 2 to move along the axial direction of the base 1 .

[0039] As an optional implementation, the adjusting member 4 is rotatably disposed on the base 1 and threadedly connected to the base 1 , and one end of the adjusting member 4 can contact the movable structure 2 , thereby driving the movable structure 2 to move along the axial direction of the base 1 .

[0040] For example, the effective length of the thread of the base 1 is set to 4 cm. When the adjusting member 4 moves upward from the lowest point of the thread 0 cm to any point between 0 and 4 cm and is fixed, the movable structure 2 is pushed to any point between 0 and 4 cm by the adjusting member 4 during the upward movement of the adjusting member 4, thereby increasing the distance between the base 1 and the movable structure 2 within the range of 0 to 4 cm.

[0041] refer to Figure 3 and Figure 4 As an optional implementation, the base 1 is provided with a guide block 5, and the movable structure 2 is provided with a guide groove 6 that matches the guide block 5, and the guide block 5 and the guide groove 6 are used to limit the rotation of the movable structure 2.

[0042] Through the cooperation between the guide groove 6 and the guide block 5, the guide groove 6 and the guide block 5 can move relatively along the inner wall of the guide groove 6; therefore, when adjusting the volume of the hatching space, the movable structure 2 rotates vertically along the cooperation direction of the guide groove 6 and the guide block 5 during the movement, thereby preventing the movable structure 2 from driving the hatching structure 3 to rotate, thereby causing damage and influence on the chicken embryo.

[0043] refer to Figure 2 and Figure 4 As an optional embodiment, it also includes a buckle 7, which is arranged at the center of the hatching structure 3 and connected to the base 1, so as to connect the center of the hatching structure 3 to the base 1.

[0044] As an optional implementation, the buckle 7 is a "T"-shaped buckle.

[0045] The "T"-shaped buckle increases the horizontal cross-section of the bottom of the hatching structure 3 to prevent the horizontal cross-section of the bottom of the hatching structure 3 from being too small to squeeze the yolk and cause it to break. At the same time, the hatching space is made smoother and suitable for the development of the chicken embryo.

[0046] refer to Figure 1 , Figure 2 and Figure 5 As an optional embodiment, it further includes a fixing ring 8 , which is disposed on the movable structure 2 and is used to connect the edge of the hatching structure 3 with the movable structure 2 .

[0047] refer to Figure 5 Optionally, the fixing ring 8 is also provided with an adjustable gap, and a gap adjusting portion 81 is provided at the adjustable gap. The gap adjusting portion 81 can be connected to the fixing rings 8 on both sides of the adjustable gap by threaded engagement, so as to change the size of the adjustable gap, thereby adjusting the locking degree of the fixing ring 8. Bolts can be used for the gap adjusting portion 81.

[0048] As an optional implementation, the base 1 and the movable structure 2 are both cylindrical, and the movable structure 2 is nested outside the base 1 .

[0049] Working principle: Assemble the variable volume chicken embryo shell incubation device for experiment, that is, use the fixing ring 8 and the buckle 7 to fix the center and edge of the incubation structure 3 to the base 1 and the movable structure 2 respectively. The distance between the movable structure 2 and the base 1 is set to the required height by the adjusting member 4. Take the fertilized eggs that have been incubated for 2.5-3 days. There is no need to identify the position of the embryo. Tap the egg body along the short axis of the egg to crack the egg. Hold the two ends of the egg close to the incubation structure 3, carefully open the egg, and let the egg white and the yolk containing the embryo slide into the incubation structure 3. Finally, cover the fixing ring 8 with sterile preservative film for sealing. At this time, the yolk can ensure a suspended posture in the incubator. Even if the position of the chicken embryo is not ideal when it falls into the incubator, the embryo will spontaneously rotate to the top. In addition, the suspended state of the yolk is also conducive to avoiding direct exposure of the embryo surface to the air. After 7 days of development of the chicken embryo, the CAM gradually develops, and its mature area can reach 65cm 2 At this time, the height of the movable structure 2 is lowered by the adjusting member 4, so that the distance between the movable structure 2 and the base 1 is reduced, thereby increasing the horizontal area of ​​the liquid surface of the yolk and egg white liquid content of the chicken embryo, giving the CAM sufficient development space. The CAM can be laid flat in the incubator along the yolk surface. At the same time, the CAM can be fully exposed, which is conducive to the later experimental operation.

[0050] The embodiments of the present application are described in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method and its core idea of ​​the present application. At the same time, changes or deformations made by those skilled in the art based on the ideas of the present application, the specific implementation methods and the scope of application of the present application, all belong to the scope of protection of the present application. In summary, the content of this specification should not be construed as a limitation on the present application.

Claims

1. An experimental variable volume chicken embryo shell incubation device, characterized in that: include: Pedestal; A movable structure is disposed on the base, and the movable structure can move along the axis direction of the base; The incubation structure is arranged between the base and the movable structure, and forms an incubation space inside; wherein one end of the incubation structure is connected to the base, and the other end is connected to the movable structure.

2. The variable volume chicken embryo shell incubation device for experiments according to claim 1, characterized in that: The cross-sectional area of ​​the incubation space along the horizontal direction increases in the direction from the base to the movable structure.

3. The variable volume chicken embryo shell incubation device for experiments according to claim 1, characterized in that: The hatching structure comprises a resilient material.

4. The variable volume chicken embryo shell incubation device for experiments according to claim 1, characterized in that: The base is provided with an adjusting member for driving the movable structure to move along the axis direction of the base.

5. The variable volume chicken embryo shell incubation device for experiments according to claim 4, characterized in that: The adjusting member is rotatably disposed on the base and is threadedly connected to the base. One end of the adjusting member can contact the movable structure, thereby driving the movable structure to move along the axial direction of the base.

6. The experimental variable volume chicken embryo shell incubation device according to any one of claims 1 to 5, characterized in that: The base is provided with a guide block, and the movable structure is provided with a guide groove matched with the guide block, and the guide block and the guide groove are used to limit the rotation of the movable structure.

7. The experimental variable volume chicken embryo shell incubation device according to any one of claims 1 to 5, characterized in that: It also includes a buckle, which is arranged at the center of the hatching structure and connected to the base, and is used to connect the center of the hatching structure to the base.

8. The variable volume chicken embryo shell incubation device for experiments according to claim 7, characterized in that: The buckle is a "T"-shaped buckle.

9. The experimental variable volume chicken embryo shell incubation device according to any one of claims 1 to 5, characterized in that: It also includes a fixing ring, which is arranged on the movable structure and is used to connect the edge of the hatching structure with the movable structure.

10. The experimental variable volume chicken embryo shell incubation device according to any one of claims 1 to 5, characterized in that: The base and the movable structure are both cylindrical, and the movable structure is nested outside the base.

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