Novel disposable liquid dispensing bag

Abstract

A novel disposable liquid dispensing bag comprises a liquid inlet open bag, a uniform mixing bag and a liquid storage bag, the liquid inlet open bag, the uniform mixing bag and the liquid storage bag are connected through a liquid inlet / outlet pipeline, and a hydrophilic sterilization filter is arranged on the liquid pipeline; the liquid inlet open bag comprises an open bag liquid outlet, a liquid inlet end pipe of the hydrophilic filter is communicated with the open bag liquid outlet, and a liquid outlet end of the hydrophilic filter is communicated with a blending bag liquid inlet in the blending bag through a silicone tube; a blending bag liquid inlet, a blending bag liquid outlet, an air inlet and an air outlet are formed in the blending bag, the blending bag liquid outlet is connected with the liquid inlet end of the hydrophilic filter through a silicone tube, the liquid outlet end of the hydrophilic filter is communicated with a liquid storage bag liquid inlet in the liquid storage bag, and the liquid storage bag liquid inlet, a liquid outlet and an air filter opening are formed in the liquid storage bag. According to the novel disposable liquid preparation bag, the yield of culture media prepared in one batch is large, and the yield of the culture media prepared in one batch is at least one time that of the culture media prepared in one batch through a single bottle.

Description

Technical Field

[0001] The invention relates to the technical field of biomedicine, in particular to a novel disposable liquid preparation bag. Background Art

[0002] In the field of cell therapy drug production, culture medium is mostly prepared in a single bottle or mixed by stirring. Individually prepared culture medium has the risk of unevenness between bottles, while mixed preparation in liquid preparation tanks is mostly carried out in stainless steel containers, which involve cleaning and sterilization operations before and after use, and lack convenience. Summary of the invention

[0003] In order to overcome the existing defects, the present invention proposes a new disposable liquid preparation bag.

[0004] A novel disposable liquid preparation bag comprises an open liquid inlet bag, a mixing bag and a liquid storage bag, wherein the open liquid inlet bag, the mixing bag and the liquid storage bag are connected via an inlet / outlet liquid pipeline, the open liquid inlet bag is a 3D bag, the mixing bag is a 2D bag, the liquid storage bag is a 2D bag, and a hydrophilic sterilizing filter is arranged on the liquid pipeline.

[0005] The liquid inlet open bag includes an open bag liquid outlet, a 3 / 8 single joint is arranged on the open bag liquid outlet, the liquid inlet end of the hydrophilic filter is connected to the 3 / 8 single joint through a first silicone tube, the liquid outlet end of the hydrophilic filter is connected to the quick connector male head and the quick connector male plug through a second silicone tube and connected to the liquid inlet on the mixing bag, wherein a thumb clip is arranged on the first silicone tube.

[0006] The mixing bag is provided with a mixing bag liquid inlet, a mixing bag liquid outlet, an air inlet and an air outlet. The air inlet is provided with a first 1 / 4 single joint, and the first air filter is connected to the first 1 / 4 single joint through a third silicone tube, wherein a Y-shaped clip is provided on the third silicone tube; the air outlet is provided with a second 1 / 4 single joint, and the second air filter is connected to the second 1 / 4 single joint through a fourth silicone tube, wherein a Y-shaped clip is provided on the fourth silicone tube, and the second air filter is connected to a one-way valve through a fifth silicone tube; the mixing bag liquid outlet is connected to the liquid inlet of the hydrophilic filter through a sixth silicone tube through a quick connector female head and a quick connector female plug, and the liquid outlet of the hydrophilic filter is connected to the quick connector male head and the quick connector male plug through a seventh silicone tube and connected to the liquid inlet on the liquid storage bag, wherein a thumb clip is provided on the sixth silicone tube. The prepared liquid is fully mixed in the new disposable liquid preparation bag, and the concentration of each reagent in the prepared liquid can be uniform.

[0007] Among them, the liquid storage bag is provided with a liquid inlet, a liquid outlet and an air filter port, wherein a 1 / 4 single joint is provided on the air filter port, and the third air filter is connected to the 1 / 4 single joint through an eighth silicone tube, wherein a Y-shaped clip is provided on the eighth silicone tube; the liquid outlet of the liquid storage bag is connected to a ninth silicone tube, and the ninth silicone tube is connected to a 1 / 4 single joint, wherein a Y-shaped clip is provided on the ninth silicone tube, and a quick connector male head and a quick connector male plug are used at the end of the silicone tube.

[0008] Preferably, the specification of the hydrophilic filter of the liquid inlet section is 1.0 / 0.65μm; the specification of the hydrophilic filter of the liquid outlet is 0.2μm. The filter can clarify and filter the prepared liquid. The 1.0 / 0.65μm (micrometer) hydrophilic filter installed at the liquid inlet can filter liquids with high viscosity, and the 0.2μm (micrometer) hydrophilic sterilizing filter installed at the liquid outlet meets the national GMP requirements. Among them, the 0.2μm (micrometer) hydrophilic sterilizing filter at the liquid outlet is connected to the silicone tube by a quick connector. If the filter is blocked, a new filter can be replaced.

[0009] The utility model provides a new type of disposable liquid preparation bag, which has a large output of a batch of prepared culture medium, which is at least twice the output of a batch of prepared culture medium in a single bottle. In addition, the culture medium prepared in a batch has been verified to achieve uniformity, which has a significant improvement advantage compared to the previous single-bottle culture medium preparation that cannot achieve uniformity, ensuring product stability.

[0010] At the same time, the use of a new disposable liquid preparation bag reduces the loss of culture medium during the preparation of culture medium, and the cost of culture medium raw materials is greatly reduced compared to the single-bottle preparation of culture medium. The culture medium prepared in a batch saves more than 1 hour in process time compared to the single-bottle preparation of culture medium, reduces the labor intensity of the operator, and saves electricity and energy.

[0011] The difficulty of operation is much lower than that of preparing culture medium in a single bottle. The operation is simple and less prone to errors. The liquid bag does not need to be cleaned and sterilized after use, thus saving a lot of water resources and avoiding pollution to the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is a schematic diagram of a new type of disposable liquid preparation bag.

[0013] Figure 2 This is a schematic diagram of the decomposition of the liquid-filled open-mouth bag.

[0014] Figure 3 Schematic diagram of the mixing bag breakdown.

[0015] Figure 4 This is a schematic diagram of the liquid storage bag. DETAILED DESCRIPTION

[0016] A novel disposable liquid dispensing bag provided by the present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0017] Figure 1-4 As shown, a novel disposable liquid preparation bag comprises an open-mouth liquid inlet bag 1, a mixing bag 2 and a liquid storage bag 3. The open-mouth liquid inlet bag 1, the mixing bag 2 and the liquid storage bag 3 are connected via an inlet / outlet liquid pipeline. The open-mouth liquid inlet bag 1 is a 3D bag, the mixing bag 2 is a 2D bag, the liquid storage bag 3 is a 2D bag, and a hydrophilic sterilizing filter 4 is arranged on the liquid pipeline.

[0018] The liquid inlet open bag 1 includes an open bag liquid outlet 11, a 3 / 8 single joint 12 is arranged on the open bag liquid outlet 11, the liquid inlet end of the hydrophilic filter 4 is connected to the 3 / 8 single joint through a first silicone tube 14, and the liquid outlet end of the hydrophilic filter 4 is connected to the quick connector male head and the quick connector male plug through a second silicone tube 15 and connected to the liquid inlet 21 on the mixing bag 2, wherein a thumb clip is arranged on the first silicone tube.

[0019] The mixing bag 2 is provided with a mixing bag liquid inlet 21, a mixing bag liquid outlet 22, an air inlet 23 and an air outlet 24. The air inlet 23 is provided with a first 1 / 4 single joint 231, and the first air filter is connected to the first 1 / 4 single joint 231 through a third silicone tube 233 through 232, wherein a Y-shaped clip is provided on the third silicone tube 233; the air outlet 24 is provided with a second 1 / 4 single joint 241, and the second air filter 244 is connected to the second 1 / 4 single joint 241 through a fourth silicone tube 245 , wherein a Y-shaped clip is arranged on the fourth silicone tube 245, and the second air filter 244 is connected to the one-way valve 247 through the fifth silicone tube 246; the liquid outlet 22 of the mixing bag is connected to the liquid inlet end of the hydrophilic filter 4 through the sixth silicone tube 221 through the quick connector female head and the quick connector female plug, and the liquid outlet end of the hydrophilic filter 4 is connected to the quick connector male head and the quick connector male plug through the seventh silicone tube 222 and connected to the liquid inlet 32 ​​on the liquid storage bag 3, wherein a thumb clip is arranged on the sixth silicone tube 221.

[0020] The liquid storage bag 3 is provided with a liquid inlet 32, a liquid outlet 33 and an air filter port 31, wherein the air filter port 31 is provided with a 1 / 4 single joint, and the third air filter 312 is connected to the 1 / 4 single joint through the eighth silicone tube 311, wherein the eighth silicone tube is provided with a Y-shaped clip; the liquid storage bag liquid outlet 33 is connected to the ninth silicone tube 331, and the ninth silicone tube 331 is connected to the 1 / 4 single joint, wherein the ninth silicone tube 331 is provided with a Y-shaped clip, and a quick connector male head and a quick connector male plug are used at the end of the silicone tube.

[0021] The specification of the hydrophilic filter 4 at the liquid inlet section is 1.0 / 0.65 μm; the specification of the hydrophilic filter 4 at the liquid outlet is 0.2 μm.

[0022] During the implementation process,

[0023] 1. First, connect the liquid inlet open bag 1 and the mixing bag 2, connect the male quick connector in the open bag with the female quick connector at the liquid inlet end of the mixing bag in a Class A environment, close the thumb clip in the open bag, the thumb clips at the liquid inlet end and the liquid outlet end of the mixing bag, and open the air filters at the air inlet end and the air outlet end of the open bag.

[0024] 2 After confirming that the connection is correct, add the reagents required for the prepared culture medium from the top of the liquid inlet open bag 1 in sequence, open the thumb clip in the open bag and the thumb clip at the liquid inlet end of the mixing bag, and let the prepared liquid flow through the first silicone tube in the open bag into the 1.0 / 0.65μm hydrophilic filter, and then flow into the mixing bag 2 through the second silicone tube. Until all the reagents flow into the mixing bag 2, prepare for the mixing operation.

[0025] 3 Before the mixing operation, close the thumb clamp at the liquid inlet end, the Y-shaped clamps at the air inlet end and the air outlet end of the mixing bag 2. Start the swing bed mixer for mixing. The solution can achieve the mixing effect in less than 10 minutes, and the mixing method is gentler than stirring, with less shear force, so the mixing efficiency is suitable for drug production.

[0026] 4 After the mixing is completed, connect the male quick connector at the liquid outlet of the mixing bag 2 to the hydrophilic sterilizing filter, and connect the hydrophilic sterilizing filter to the female quick connector at the liquid inlet of the liquid storage bag 3, and close the thumb clamp at the liquid inlet of the liquid storage bag and the Y-shaped clamp at the liquid outlet. Open the Y-shaped clamp at the air filter port of the liquid storage bag 3, the thumb clamp at the liquid outlet of the mixing bag 2, and the thumb clamp at the liquid inlet of the liquid storage bag 3, so that the prepared culture medium flows from the pipeline at the liquid outlet of the mixing bag 2 through the 0.2μm (micrometer) hydrophilic sterilizing filter, and flows into the liquid storage bag 3 through the pipeline after sterilization and filtration.

[0027] 5. Start filling by opening the Y-shaped clip at the liquid outlet end of the liquid storage bag 3, so that the sterilized and filtered culture medium can be filled into the corresponding container through the pipeline.

[0028] Finally, it should be noted that the above embodiments are only used to describe the technical solution of the present invention rather than to limit the technical method. The present invention can be extended to other modifications, changes, applications and embodiments in application, and therefore all such modifications, changes, applications and embodiments are considered to be within the scope of the present invention.

Claims

1. A new type of disposable liquid preparation bag, characterized in that: The liquid preparation bag comprises an open-mouth liquid inlet bag (1), a mixing bag (2) and a liquid storage bag (3); the open-mouth liquid inlet bag (1), the mixing bag (2) and the liquid storage bag (3) are connected via an inlet / outlet liquid pipeline; the open-mouth liquid inlet bag (1) is a 3D bag, the mixing bag (2) is a 2D bag, and the liquid storage bag (3) is a 2D bag; a hydrophilic filter (4) is arranged on the liquid pipeline; The liquid inlet open bag (1) comprises an open bag liquid outlet (11), a 3 / 8 single joint is arranged on the open bag liquid outlet (11), the liquid inlet end of the hydrophilic filter (4) is connected to the 3 / 8 single joint (12) through a first silicone tube (14), the liquid outlet end of the hydrophilic filter (4) is connected to a quick connector male head and a quick connector male plug through a second silicone tube (15) and is connected to a mixing bag liquid inlet (21) on the mixing bag (2), wherein a thumb clip is arranged on the first silicone tube; The mixing bag (2) is provided with a mixing bag liquid inlet (21), a mixing bag liquid outlet (22), an air inlet (23) and an air outlet (24); the air inlet (23) is provided with a first 1 / 4 single joint (231); the first air filter is connected to the first 1 / 4 single joint (231) through a third silicone tube (233); a Y-shaped clip is provided on the third silicone tube (233); a second 1 / 4 single joint (241) is provided on the air outlet (24); the second air filter (244) is connected to the second 1 / 4 single joint (241) through a fourth silicone tube (245); 1), wherein a Y-shaped clip is arranged on the fourth silicone tube (245), and the second air filter (244) is connected to a one-way valve (247) through a fifth silicone tube (246); the liquid outlet (22) of the mixing bag is connected to the liquid inlet of the hydrophilic filter (4) through a sixth silicone tube (221) through a quick connector female head and a quick connector female plug, and the liquid outlet of the hydrophilic filter (4) is connected to the quick connector male head and the quick connector male plug through a seventh silicone tube (222) and the liquid inlet (32) of the liquid storage bag on the liquid storage bag (3), wherein a thumb clip is arranged on the sixth silicone tube (221); The liquid storage bag (3) is provided with a liquid storage bag liquid inlet (32), a liquid outlet (33) and an air filter port (31), wherein a 1 / 4 single joint is provided on the air filter port (31), and the third air filter (312) is connected to the 1 / 4 single joint via an eighth silicone tube (311), wherein a Y-shaped clip is provided on the eighth silicone tube; the liquid storage bag liquid outlet (33) is connected to a ninth silicone tube (331), and the ninth silicone tube (331) is connected to the 1 / 4 single joint, wherein a Y-shaped clip is provided on the ninth silicone tube (331), and a quick connector male head and a quick connector male plug are used at the end of the silicone tube; The specification of the hydrophilic filter (4) at the liquid inlet end is 1.0 / 0.65 μm; the specification of the hydrophilic filter (4) at the liquid outlet end is 0.2 μm.

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