Extraction reagent assembly

Abstract

The utility model belongs to the technical field of biological sample treatment, and provides a reagent extraction assembly which comprises a kit and a magnetic rod group, the magnetic rod group is mounted above the kit and can move in the kit; the kit comprises a plurality of hole groups, the hole groups are arranged side by side, the column number of the hole groups is not less than one, and the row number is not less than one; each hole group is provided with a sample hole, four washing liquid holes and two elution holes, the four washing liquid holes are arranged in a shape like a Chinese character'tian ', the two elution holes are arranged on one side of the four washing liquid holes side by side in a shape like a Chinese character'ri', the sample hole is arranged on the other side of the four washing liquid holes, and the width of the sample hole corresponds to the total width of two adjacent washing liquid holes. According to the utility model, a plurality of hole groups are arranged side by side, so that a plurality of samples are allowed to be processed at the same time, and the experiment processing capacity and efficiency are remarkably improved. Due to the specific design of the interior of each hole group, the sample holes have enough stirring space, meanwhile, the washing and elution processes are more efficient, and the experiment time is shortened.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological sample processing, and particularly relates to an extraction reagent assembly. Background Art

[0002] In biological science research and clinical diagnosis, it is often necessary to extract specific components from biological samples. Traditional extraction methods are cumbersome, inefficient, and easily affected by human operation errors.

[0003] In order to improve the efficiency, Chinese Patent CN202020379097.2 discloses a deep well plate for a nucleic acid extractor, which solves the problems of poor applicability such as small deep well openings and small volumes in the prior art. The adopted technical solution is that the deep well at least includes a first deep well and a second deep well, and the volume of the first deep well is larger than that of the second deep well. The effect is that different volumes of deep wells are set in a deep well plate, with large-volume deep wells and small-volume deep wells, which is convenient to meet different needs during nucleic acid extraction, improves the applicability of the deep well plate of the nucleic acid extractor, can reduce the number of deep well plate specifications, not only reduces the production cost of the deep well plate, but also facilitates the operator to use the deep well plate and operate, and meets more nucleic acid extraction operation requirements. However, the involved deep well plate or reagent strip has a strip-shaped lysis hole, and the lysis hole is used to allow the magnetic rod sleeve to move along the length direction of the lysis hole after the magnetic rod sleeve extends into the lysis hole along the depth direction of the lysis hole. Summary of the Utility Model

[0004] In order to overcome the problems existing in the prior art, the purpose of the utility model is to provide an extraction reagent assembly.

[0005] The technical solution adopted by the utility model to solve its technical problems is: an extraction reagent assembly, including: a reagent kit and a magnetic rod group; the magnetic rod group is installed above the reagent kit and the magnetic rod group can move in the reagent kit; the reagent kit includes a group of holes, multiple groups of holes are arranged side by side, and the number of columns of the group of holes is not less than one column, and the number of rows is not less than one row; each group of holes is provided with a sample hole, four washing holes, and two elution holes. The four washing holes are arranged in a "field" shape, and the two washing and dragging holes are arranged side by side in a "day" shape on one side of the four washing holes, and the sample hole is arranged on the other side of the four washing holes. The width of the sample hole corresponds to the total width of two adjacent washing holes; the number of the magnetic rod groups corresponds to the number of columns of the group of holes, and the magnetic rod groups are connected by a connecting piece. The magnetic rod group includes multiple rod bodies, and the number of the rod bodies corresponds to the number of rows of the group of holes. During the working process, each rod body corresponds to a group of holes.

[0006] Its main working principle is as follows: The kit contains multiple well groups, and each well group has specific functional wells, including sample wells, washing wells, and elution wells. This design enables each well group to independently perform sample washing and elution operations, thereby improving experimental efficiency and flexibility. The specific arrangement of the "field"-shaped washing wells and the "day"-shaped elution wells in the well group ensures the uniform distribution and effective circulation of the washing liquid and elution liquid within the well group. Moreover, since the width of the sample well corresponds to the total width of two adjacent washing wells, multi-directional stirring can be performed when stirring the sample well. The magnetic bar group is installed above the kit, and the number of magnetic bars matches the number of columns of the well group, and the number of bar bodies corresponds to the number of rows of the well group. This design allows the magnetic bar group to precisely control the liquid flow within each well group and achieve precise washing and elution operations.

[0007] During operation, in the sample addition stage, the biological or chemical sample to be processed is added to the sample well, an appropriate amount of washing liquid is added to the washing well, and an appropriate amount of elution liquid is added to the elution well.

[0008] In the stirring stage, the bar body is inserted into the sample well and stirred evenly along the circumferential direction in the sample well. In the washing stage, through the movement of the magnetic bar group, the bar body is moved to the washing well filled with the washing liquid for washing to remove impurities in the sample. The washing liquid is evenly distributed in the "field"-shaped washing wells, improving the washing efficiency.

[0009] In the elution stage, the magnetic bar group moves again, allowing the bar body to be inserted into the elution well to bring the elution liquid into contact with the sample and elute the target substance from the sample. The elution liquid can more effectively contact the sample in the "day"-shaped elution wells, improving the elution efficiency.

[0010] Preferably, the four washing wells are respectively two protein washing wells and two rinsing wells. The two protein washing wells are arranged on the same side, and the two rinsing wells are arranged on the other side opposite to the two protein washing wells.

[0011] Preferably, the volume of the sample well is not less than 25 ml, and the volume of each washing well and each elution well is not less than 3.5 ml.

[0012] Preferably, the bottom of each washing well and each elution well protrudes outward, and the end of each bar body corresponds to the bottom of each washing well and each elution well protruding outward.

[0013] Preferably, the number of columns of the well group is not less than two columns, and the number of rows of the well group is not less than four rows.

[0014] Preferably, the magnetic bar further includes a connecting rod, and the bottom of the connecting rod is provided with multiple bar bodies arranged at equal distances.

[0015] Preferably, a clip is provided at the end of the connecting rod, and the connecting rod is clamped and matched with the connecting piece through the clip.

[0016] Preferably, both sides of the connecting rod are provided with corrugated protrusions, and the connecting rod is limitedly matched with the connecting piece through the corrugated protrusions.

[0017] Preferably, stirring bars are arranged on the surface of the rod body, and the stirring bars are arranged along the length direction of the rod body.

[0018] Compared with the prior art, the beneficial effects of the utility model are:

[0019] The utility model allows multiple samples to be processed simultaneously by arranging multiple well groups side by side, which significantly improves the processing capacity and efficiency of the experiment. The specific design inside each well group allows the sample well to have sufficient stirring space, while making the washing and elution processes more efficient and reducing the experimental time; the introduction of the magnetic rod group makes the operation process simpler, and the washing and elution processes can be automatically completed by moving the magnetic rod group, reducing the complexity of manual operation and human errors. At the same time, the precise control of the magnetic rod group ensures the standardization of the experimental process and improves the reliability and repeatability of the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 Schematic diagram of the overall extraction reagent assembly.

[0022] 1. Test kit; 10. Well group; 100. Sample well; 101. Washing solution well; 1010. Protein washing well; 1011. Rinse well; 102. Elution well; 2. Magnetic rod group; 20. Rod body; 21. Connecting rod; 22. Clip; 23. Corrugated protrusion; 24. Stirring bar. DETAILED DESCRIPTION

[0023] In order to more clearly understand the above-mentioned purposes, features and advantages of the utility model, the utility model is described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that, in the absence of conflict, the embodiments of the present application and the features in the embodiments can be combined with each other. In the following description, many specific details are set forth in order to fully understand the utility model, and the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0025] Example 1

[0026] This embodiment provides an extraction reagent assembly designed for automated processing of biological or chemical samples. Figure 1 As shown, the assembly includes a reagent box 1 and a magnetic rod set 2. The magnetic rod set 2 is installed above the reagent box 1 and can move inside the reagent box 1 to achieve automated processing of samples.

[0027] Among them, there are multiple well groups 10 inside the kit 1, and these well groups 10 are arranged side by side in a multi-column and multi-row form. In this embodiment, the number of columns of the well groups 10 is not less than two columns, and the number of rows is not less than four rows. Each well group 10 includes a sample well 100, four washing wells 101, and two elution wells 102. Each well group 10 is provided with a sample well 100 with a volume of not less than 25 ml for storing the sample to be processed. The four washing wells 101 are arranged in a "field" shape. Among them, two protein washing wells 1010 are arranged on the same side for adding protein washing solution; two rinsing wells 1011 are arranged on the other side of the two protein washing wells 1010 for adding rinsing solution. The volume of each washing well 101 is not less than 3.5 ml. The two elution wells 102 are arranged side by side in a "day" shape on one side of the four washing wells 101, with a volume of not less than 3.5 ml, and are used to add elution solution to elute the target substance from the sample. The sample well 100 is arranged on the other side of the four washing wells 101, and its width corresponds to the total width of two adjacent washing wells 101, ensuring that the magnetic rod group 2 can accurately align with each well group 10 during movement. And because the width of the sample well 100 corresponds to the total width of two adjacent washing wells 101, multi-directional stirring can be carried out when stirring the sample well 100. The multiple well groups 10 are arranged in a matrix form inside the kit 1, facilitating the movement and operation of the magnetic rod group 2 inside the kit 1.

[0028] In this embodiment, since the well groups 10 are in two columns, two groups of magnetic rod groups 2 are also provided. Each group of magnetic rod groups 2 corresponds to one column of well groups 10 and includes multiple rod bodies 20. The number of rod bodies 20 corresponds to the number of rows of the well groups 10. In this embodiment, since the well groups 10 are in four rows, each group of magnetic rod groups 2 includes four rod bodies 20. Each rod body 20 corresponds to one well group 10 and is used to correspond to the well positions in the well group 10 during operation. The two groups of magnetic rod groups 2 are connected by a connecting piece, and this connecting piece can be a fixed connecting bracket to ensure the stability and consistency of the magnetic rod group 2 during movement. The connecting piece is made of a strong and durable material and can bear the weight of the magnetic rod group 2 and the force during movement.

[0029] During work, in the sample addition stage, the biological or chemical sample to be processed is added to the sample well 100, an appropriate amount of washing solution is added to the washing wells 101, and an appropriate amount of elution solution is added to the elution wells 102.

[0030] In the stirring stage, the rod body 20 is extended into the sample well 100 and stirred evenly along the circumferential direction in the sample well 100. In the washing stage, through the movement of the magnetic rod group 2, the rod body 20 is moved to the washing wells 101 filled with washing solution for washing to remove impurities in the sample. The washing solution is evenly distributed in the "field"-shaped washing wells 101, improving the washing efficiency.

[0031] In the elution stage, the magnetic bar group 2 moves again, inserting the bar body 20 into the elution holes 102 to bring the eluent into contact with the sample and elute the target substance from the sample. The eluent can contact the sample more effectively in the "day"-shaped arranged elution holes 102, improving the elution efficiency.

[0032] In some alternative embodiments, the magnetic bar further includes a connecting rod 21, and a plurality of bar bodies 20 are arranged at equal intervals at the bottom of the connecting rod 21. A clamping piece 22 is provided at the end of the connecting rod 21, and the magnetic bar group 2 is firmly held in place during movement by the clamping cooperation between the clamping piece 22 and the connecting member.

[0033] In some alternative embodiments, corrugated protrusions 23 are provided on both sides of the connecting rod 21, and a limiting fit is formed between these corrugated protrusions 23 and the connecting member, further enhancing the stability of the magnetic bar group 2 during movement.

[0034] Preferably, stirring bars 24 are provided on the surface of the bar body 20. The stirring bars 24 are arranged along the length direction of the bar body 20. In this way, the stirring area is increased, thereby improving the stirring efficiency.

[0035] The above are only the preferred embodiments of the present invention, and do not impose any form of limitation on the present invention. Therefore, any modification, equivalent change, and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. An extraction reagent assembly, characterized in that: Including: A reagent kit and a magnetic bar set; the magnetic bar set is installed above the reagent kit and the magnetic bar set is movable within the reagent kit; The reagent kit includes a set of holes, a plurality of the sets of holes are arranged side by side, and the number of columns of the sets of holes is not less than one column and the number of rows is not less than one row; each set of holes is provided with a sample hole, four washing holes and two elution holes, the four washing holes are arranged in a "field" shape, the two elution holes are arranged side by side in a "day" shape on one side of the four washing holes, the sample hole is arranged on the other side of the four washing holes, and the width of the sample hole corresponds to the total width of two adjacent washing holes; The number of the magnetic bar sets corresponds to the number of columns of the sets of holes, and the magnetic bar sets are connected by a connecting member, the magnetic bar set includes a plurality of rod bodies, the number of the rod bodies corresponds to the number of rows of the sets of holes, and during the working process, each rod body corresponds to a set of holes.

2. The extraction reagent assembly according to claim 1, wherein The four washing holes are respectively two protein washing holes and two rinsing holes, the two protein washing holes are arranged on the same side, and the two rinsing holes are arranged on the other side of the two protein washing holes.

3. The extraction reagent assembly according to claim 2, wherein The volume of the sample hole is not less than 25 ml, and the volume of each washing hole and each elution hole is not less than 3.5 ml.

4. The extraction reagent assembly according to claim 1, wherein The bottom of each washing hole and each elution hole protrudes outward, and the end of each rod body corresponds to the bottom of each washing hole and each elution hole protruding outward.

5. The extraction reagent assembly according to claim 1, wherein The number of columns of the sets of holes is not less than two columns, and the number of rows of the sets of holes is not less than four rows.

6. The extraction reagent assembly according to claim 1, wherein The magnetic bar further includes a connecting rod, and the connecting rod is arranged at the bottom of the plurality of rod bodies arranged at equal intervals.

7. The extraction reagent assembly according to claim 6, wherein The end of the connecting rod is provided with a clip, and the connecting rod is clamped and matched with the connecting member through the clip.

8. The extraction reagent assembly according to claim 6, wherein Both sides of the connecting rod are provided with corrugated protrusions, and the connecting rod is in limit fit with the connecting member through the corrugated protrusions.

9. The extraction reagent assembly according to claim 6, wherein The surface of the rod body is provided with stirring bars, and the stirring bars are arranged along the length direction of the rod body.

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