Separation and purification device for lactobacillus of siniperca chuatsi

Abstract

The utility model discloses a siniperca chuatsi lactobacillus separation and purification device which comprises a culture dish body, a culture dish cover and a marking plate cover, the culture dish cover inner wall is in threaded connection with the culture dish body outer wall; the center of the top surface of the culture dish cover is provided with a connecting hole and a semicircular inoculation opening taking the connecting hole as the center; a connecting column extending upwards is arranged in the center of the marking-off plate cover, penetrates through the connecting hole upwards and is in running fit with the connecting hole; the top surface of the marking-off plate cover is matched with the bottom surface of the culture dish cover, and an arc-shaped marking-off hole taking the connecting column as the center is formed in the marking-off plate cover; the central angle of the scribing hole is smaller than 180 degrees. When the device disclosed by the utility model is used for purifying lactic acid bacteria, the opening of the culture dish is as small as possible during inoculation, and infectious microbes entering lactic acid bacteria are reduced, so that the accuracy of experimental results is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of lactic acid bacteria, in particular to a device for separating and purifying stinky mandarin fish lactic acid bacteria. Background Art

[0002] Stinky mandarin fish is a traditional delicacy in Huizhou, Anhui Province. Most people who see it for the first time are afraid to eat it because of the smell of mandarin fish, which is a bit worrying. This is not rotten, but a special smell produced by the action of fermentation bacteria. It smells like stinky, but has a unique fragrance after cooking. In order to make stinky mandarin fish, bacterial strain control is required, which generally inhibits the growth of harmful bacteria and promotes the growth of lactic acid bacteria that are beneficial to health.

[0003] In the existing separation and purification of stinky mandarin fish lactic acid bacteria, the strain is first diluted with sterile water, and then the lactic acid bacteria are separated, and then purified. The purification is usually carried out by streaking in a culture dish. However, when streaking in a culture dish, it is easy to introduce bacteria in the air into the lactic acid bacteria in the culture dish, resulting in deviation in purification. Utility Model Content

[0004] In view of the problems and shortcomings in the prior art, the utility model provides a device for separating and purifying lactobacillus from stinky mandarin fish.

[0005] The technical solution of this utility model is as follows:

[0006] A device for separating and purifying lactobacillus from stinky mandarin fish, comprising a culture dish body, a culture dish cover and a ruled plate cover;

[0007] The inner wall of the culture dish cover is threadedly connected to the outer wall of the culture dish body;

[0008] A connecting hole and a semicircular inoculation port centered on the connecting hole are provided at the center of the top surface of the culture dish cover; a connecting column extending upward is provided at the center of the scoring plate cover, and the connecting column passes upward through the connecting hole and rotates therewith; the top surface of the scoring plate cover cooperates with the bottom surface of the culture dish cover, and is provided with an arc-shaped scoring hole centered on the connecting column; the central angle of the scoring hole is less than 180°.

[0009] When in use, the marking plate cover is driven to rotate by rotating the connecting column. After the marking holes on the marking plate cover are rotated to below the inoculation hole and all the marking holes leak out of the inoculation port, the inoculation loop dipped in the smelly mandarin fish soft acid bacteria is used to mark the culture dish along the shape of the marking holes. After completing the marking of half of the culture dish, the culture dish cover is rotated so that the inoculation port is located on the half of the culture dish that is not inoculated, and the above-mentioned marking action is repeated again to complete the marking purification inside the entire culture dish. Make the opening of the culture dish as small as possible during inoculation to reduce the entry of miscellaneous bacteria into the lactobacillus, thereby improving the accuracy of the experimental results. After completing the marking, the connecting column is rotated again to rotate the marking hole to below the non-inoculation port of the culture surface cover, the culture dish is sealed, and then the culture dish is placed in an incubator for colony culture.

[0010] In a specific embodiment, a bearing is provided in the connecting hole, and the connecting column is rotatably connected to the connecting hole through the bearing. An elastic circlip for the shaft is provided on the connecting column, which is located above the culture dish cover and is used to axially position the scribe plate cover. In a specific implementation, the elastic circlip for the shaft is installed in a retaining ring groove provided on the connecting column.

[0011] In a specific embodiment, the outer periphery of the scribe plate cover is provided with two limiting flanges, which are symmetrically arranged with the connecting column as the center and extend radially outward respectively, and the inner side wall of the culture dish cover is evenly distributed with four limiting grooves that cooperate with the limiting flanges. The limiting flange is semicircular and extends radially outward, and the limiting groove is an arc groove that cooperates with it.

[0012] The limiting flange and limiting groove are used to position the scoring plate cover when the scoring plate cover is rotated to the point where the scoring hole is below the inoculation port to prevent it from rotating freely, that is, the scoring plate cover is positioned by the engagement of the limiting flange and the limiting groove when it is not rotated 180°.

[0013] In a specific embodiment, the central angle of the scribing hole is 100-150°. The purpose is to ensure the length of the scribing as much as possible. In order to increase the number of scribing inoculations, three scribing holes are arranged radially at intervals along the scribing plate cover, and the central angles of the three scribing holes coincide. In order to facilitate the inoculation ring to scribing along the scribing groove, the radial width of the inoculation port is greater than the width of the three scribing holes.

[0014] In a specific embodiment, a rotating handle is provided on the top of the connecting column, and the rotating handle is ring-shaped, so that the user can rotate the marking plate cover conveniently.

[0015] The beneficial effects of the utility model are:

[0016] A semicircular inoculation port is provided on the culture dish cover, and a scoring plate cover is rotatably connected under the culture dish cover, the scoring plate cover is provided with an arc-shaped scoring hole with a central angle less than 180°, the scoring hole is rotated to the bottom of the inoculation port by rotating the scoring plate cover, and scoring purification is performed along the shape of the scoring hole, so that the opening of the culture dish during inoculation is as small as possible, reducing the entry of miscellaneous bacteria into the lactobacillus, thereby improving the accuracy of the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A perspective view of an embodiment;

[0018] Figure 2 A half-section view of an embodiment;

[0019] Figure 3 An exploded view of an embodiment;

[0020] Figure 4 A stereoscopic image of a culture dish cover from the bottom perspective;

[0021] Figure 5 A three-dimensional view of the scoring plate cover rotated to the scoring position. DETAILED DESCRIPTION

[0022] The following will further describe the technical means adopted to achieve the predetermined inventive purpose of the present utility model in conjunction with the accompanying drawings in the embodiments of the present utility model.

[0023] See also Figure 1-Figure 3 As shown, a device for separating and purifying lactobacillus from smelly mandarin fish comprises a culture dish body 1, a culture dish cover 2 and a ruled plate cover 3;

[0024] See also Figure 2 As shown, the inner wall of the culture dish cover 2 is threadedly connected to the outer wall of the culture dish body 1;

[0025] See also Figure 2 and Figure 3 As shown, a connecting hole 21 and a semicircular inoculation port 22 with the connecting hole 21 as the center are provided at the center of the top surface of the culture dish cover 2; a connecting column 31 extending upward is provided at the center of the scoring plate cover 3, and the connecting column 31 passes through the connecting hole 21 upward and rotates therewith; the top surface of the scoring plate cover 3 cooperates with the bottom surface of the culture dish cover 2, and is provided with an arc-shaped scoring hole 33 with the connecting column 31 as the center; the central angle of the scoring hole 33 is less than 180°.

[0026] See also Figure 5As shown, when in use, the scoring plate cover 3 is driven to rotate by rotating the connecting column 31. After the scoring holes 33 on the scoring plate cover 3 are rotated to below the inoculation holes and all the scoring holes 33 are leaking out of the inoculation port 22, an inoculation loop dipped in stinky mandarin fish soft acid bacteria is used to score along the shape of the scoring holes 33 in the culture dish. After completing the scoring of half of the culture dish, the culture dish cover 2 is rotated so that the inoculation port 22 is located on the half of the culture dish that has not been inoculated. The above-mentioned scoring action is repeated again to complete the scoring purification of the entire culture dish. The opening of the culture dish is made as small as possible during inoculation to reduce the entry of miscellaneous bacteria into the lactobacillus, thereby improving the accuracy of the experimental results. When the scoring is completed, the connecting column 31 is rotated again to rotate the scoring holes 33 to below the non-inoculation port 22 of the culture surface cover, see Figure 1 As shown, the culture dish is sealed and then placed in an incubator for colony culture.

[0027] See also Figure 2 As shown, in a specific embodiment, a bearing 5 is provided in the connecting hole 21, and the connecting column 31 is rotatably connected to the connecting hole 21 through the bearing 5. A shaft elastic circlip 4 is provided on the connecting column 31, which is located above the culture dish cover 2 and is used to axially position the scribe plate cover 3. In a specific implementation, the shaft elastic circlip 4 is installed in a retaining ring groove provided on the connecting column 31.

[0028] See also Figure 3 As shown, in a specific embodiment, two limiting flanges 32 are provided on the outer periphery of the scribe plate cover 3, which are symmetrically arranged with the connecting column 31 as the center and extend radially outward respectively, see Figure 4 As shown, four limiting grooves 23 are evenly distributed on the inner wall of the culture dish cover 2 and cooperate with the limiting flange 32. The limiting flange 32 is semicircular and extends radially outward, and the limiting grooves 23 are arc grooves that cooperate with it.

[0029] The limiting flange 32 and the limiting groove 23 are used to position the scoring plate cover 3 when the scoring plate cover 3 is rotated to the point where the scoring hole 33 is located below the inoculation port 22 to prevent it from rotating freely, that is, the scoring plate cover 3 is positioned by the engagement of the limiting flange 32 and the limiting groove 23 when it is not rotated 180°.

[0030] See also Figure 3 As shown, in a specific embodiment, the central angle of the scribing hole 33 is 100-150°. The purpose is to ensure the length of the scribing as much as possible. In order to increase the number of scribing inoculations, three scribing holes 33 are arranged radially at intervals along the scribing plate cover 3, and the central angles of the three scribing holes 33 coincide. In order to facilitate the inoculation ring to scribing along the scribing groove, the radial width of the inoculation port 22 is greater than the width of the three scribing holes 33.

[0031] See also Figure 1 , Figure 2 and Figure 5As shown, in a specific embodiment, a rotating handle 6 is provided on the top of the connecting column 31, and the rotating handle 6 is annular, so that the user can rotate the marking plate cover 3 conveniently.

[0032] The above are preferred implementation modes of the present invention, but the present invention is not limited to the above implementation modes and examples. Various changes, equivalent substitutions, improvements, etc. made within the scope of knowledge possessed by those skilled in the art without departing from the concept of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for separating and purifying lactobacilli from stinky mandarin fish, characterized in that: It comprises a culture dish body (1), a culture dish cover (2) and a streaking plate cover (3); The inner wall of the culture dish cover is threadedly connected to the outer wall of the culture dish body; A connecting hole (21) and a semicircular inoculation port (22) centered on the connecting hole (21) are provided at the center of the top surface of the culture dish cover (2); a connecting column (31) extending upward is provided at the center of the scoring plate cover (3), and the connecting column passes through the connecting hole (21) upward and rotates with the connecting hole; the top surface of the scoring plate cover (3) is matched with the bottom surface of the culture dish cover (2), and a circular arc-shaped scoring hole (33) centered on the connecting column is provided thereon; the central angle of the scoring hole is less than 180°.

2. The separation and purification device according to claim 1, characterized in that: A bearing (5) is provided in the connection hole (21), and the connection column (31) is rotatably connected to the connection hole (21) via the bearing (5).

3. The separation and purification device according to claim 2, characterized in that: The connecting column (31) is provided with an elastic retaining ring (4) for the shaft, which is located above the culture dish cover (2) and is used for axial positioning of the scoring plate cover (3).

4. The separation and purification device according to any one of claims 1 to 3, characterized in that: The outer periphery of the scribe plate cover (3) is provided with two limiting flanges (32), which are symmetrically arranged with the connecting column (31) as the center and extend radially outward respectively. Four limiting grooves (23) matching with the limiting flanges (32) are evenly distributed on the inner side wall of the culture dish cover (2).

5. The separation and purification device according to claim 4, characterized in that: The limiting flange (32) is semicircular and extends radially outward, and the limiting groove (23) is a circular arc groove that matches the limiting flange.

6. The separation and purification device according to claim 4, characterized in that: The central angle of the scribed hole (33) is 100-150°.

7. The separation and purification device according to claim 5, characterized in that: Three of the scribing holes (33) are arranged at intervals along the radial direction of the scribing plate cover (3), and the central angles of the three scribing holes coincide.

8. The separation and purification device according to claim 7, characterized in that: The radial width of the inoculation port (22) is greater than the width of the three scoring holes (33).

9. The separation and purification device according to claim 4, characterized in that: A rotating handle (6) is provided on the top of the connecting column (31).

10. The separation and purification device according to claim 9, characterized in that: The rotating handle (6) is ring-shaped.

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