Miniature device for fish egg incubation

Abstract

The utility model discloses a miniature device for roe incubation. The miniature device comprises a culture plate, a cover plate, a plurality of gas regulating valves and a gas pump assembly, wherein the cover plate movably covers the culture plate in a sealing manner; a plurality of culture grooves are formed in the culture plate, micropores are formed in the positions, corresponding to the culture grooves, of the cover plate, the gas adjusting valves are provided with gas inlet ends and gas outlet ends, each micropore is correspondingly connected with the gas outlet end of one gas adjusting valve, or the micropores are grouped, each group is connected with the gas outlet end of one gas adjusting valve, or the micropores are grouped into groups, and each group is connected with the gas outlet end of one gas adjusting valve. All the micropores are jointly connected with the gas outlet end of a gas regulating valve, the gas inlet end of the gas regulating valve is communicated with a gas pump assembly, and the gas regulating valve regulates and controls the ventilation volume of the culture tanks in the micropores through the gas shunting effect. According to the utility model, the cover plate of the culture plate is provided with the plurality of micropores corresponding to the culture grooves, and the gas regulating valve regulates and controls the ventilatory capacity of the culture grooves in the micropores through a gas shunting effect, so that batch experiments can be simultaneously carried out in a small space, and the use is convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of hatching experimental equipment, in particular to a micro device for hatching fish eggs. Background Art

[0002] In the field of scientific research, drug activity or toxicity screening studies, due to limited conditions, the available doses of some drugs to be studied are very small. However, the volume of existing experimental devices is at least in liters, and such devices are not suitable for experiments with microliters as the unit of measurement. Laboratories sometimes use culture dishes as devices for incubating fish eggs and raising fry, but because the space of culture dishes is relatively single, if experiments under different conditions need to be carried out at the same time, multiple culture dishes need to be used, which is not conducive to the experimenters' observation, comparison and recording of experimental results.

[0003] In the production field, especially the hatching and breeding of precious and rare fish species, due to limited conditions, the number of fish eggs is small. Once a few fish eggs are infected with bacteria or viruses, all fish eggs will die. Existing equipment is not suitable for hatching a small number of fish eggs.

[0004] In addition, the existing device requires the experimenter to manually fill the water with air to avoid the growth of bacteria (especially mold) and the developmental delay or even death of the fertilized eggs caused by lack of oxygen. This operation process is relatively cumbersome. Utility Model Content

[0005] The utility model aims to provide a micro device for hatching fish eggs, which can carry out batch experiments in a small space at the same time, ensure the water quality without changing the water, and improve the success rate of hatching and the survival rate of young fish.

[0006] To achieve the above-mentioned purpose, the solution of the utility model is: a micro-device for hatching fish eggs, comprising a culture plate and a movable sealing cover, a cover plate arranged on the culture plate, a plurality of gas regulating valves and an air pump assembly; the culture plate is provided with a plurality of culture grooves, the cover plate is provided with micropores at positions corresponding to the culture grooves, the gas regulating valve is provided with an air inlet end and an air outlet end, each micropore is respectively connected to the air outlet end of a gas regulating valve, or, a plurality of micropores are grouped and each group is connected to the air outlet end of a gas regulating valve, or, all the micropores are commonly connected to the air outlet end of a gas regulating valve, the air inlet end of the gas regulating valve is connected to the air pump assembly, and the gas regulating valve regulates the ventilation volume of the culture groove in the micropore through the gas diversion effect.

[0007] In a preferred embodiment, the gas regulating valve comprises a first micro-spray drip irrigation head, a needle and a hose, wherein the first micro-spray drip irrigation head comprises a drip irrigation head body and an adjusting cap, a plug connector is provided at one end of the drip irrigation head body, a hollow airway is provided inside the plug connector, and the airway extends to the other end of the drip irrigation head body, the adjusting cap is sealingly rotatably arranged at the other end of the drip irrigation head body, and is used to adjust the gas flow in the airway in the drip irrigation head body, one end of the hose is connected to the needle plug at one end of the needle, and the other end of the hose is connected to the plug connector at one end of the drip irrigation head body, and an air guide hole is provided on the needle stem of the needle.

[0008] Preferably, the air guide hole of the needle stem is opened at a position between 2 mm and 6 mm away from the needle plug.

[0009] In a preferred embodiment, the air pump assembly includes an air pump, a first air tube, a second micro-spray drip irrigation head and a third micro-spray drip irrigation head, wherein the first air tube is fixedly arranged on the surface of the cover plate, the needle tip at the other end of the needle passes through the first air tube and the micropore in sequence and is located in the culture trough, the air guide hole of the needle stem is located in the first air tube, the air inlet at one end of the first air tube is connected to the air pump through the second micro-spray drip irrigation head, and the air outlet at the other end of the first air tube is connected to the third micro-spray drip irrigation head.

[0010] The preferred embodiment further includes a three-way joint, a second air pipe and a hose, wherein the air inlet at one end of the first air pipe is connected to the first joint of the three-way joint, the second joint of the three-way joint is connected to the air pump through the second air pipe, and the third joint of the three-way joint is connected to the plug joint of the drip irrigation head body of the second micro-spray drip irrigation head through the hose.

[0011] Preferably, the needle tip is arranged at a position between 1 mm and 10 mm from the bottom of the culture tank.

[0012] The preferred embodiment further includes at least one bracket, which is laterally mounted on the surface of the cover plate, and the cross-section of the bracket is U-shaped. The first air pipe is fixedly penetrated in the bracket. When one bracket is set, each micropore is connected to a corresponding outlet end of a gas regulating valve. When two or more brackets are set, multiple brackets are laterally mounted in parallel on the surface of the cover plate, and multiple micropores are grouped and each micropore in each group is connected to a corresponding outlet end of a gas regulating valve.

[0013] In a preferred embodiment, the culture grooves are circular in shape, and a plurality of culture grooves are evenly distributed on the culture plate, and the microholes of the cover plate are opened at positions corresponding to the centers of the culture grooves.

[0014] Preferably, the diameter of the micropores is between 0.5 mm and 2 mm.

[0015] A preferred embodiment further comprises a lighting device, which is arranged above the culture plate to provide light required for hatching.

[0016] After adopting the above scheme, the beneficial effect of the utility model is that: the utility model opens a plurality of microholes corresponding to the culture tanks on the cover plate of the culture plate, each microhole is respectively connected to the gas outlet end of a gas regulating valve, or a plurality of microholes are grouped and each group is connected to the gas outlet end of a gas regulating valve, or all the microholes are connected to the gas outlet end of a gas regulating valve. The gas regulating valve regulates the ventilation volume of the culture tank in the microhole through the gas diversion effect. The structure is simple, and the water quality is guaranteed without changing the water, thereby improving the success rate of hatching and the survival rate of young fish. In addition, a plurality of culture tanks enable batch experiments to be carried out simultaneously in a small space, which is convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 It is a schematic diagram of the cover plate movable sealing cover of the utility model being arranged on the culture plate;

[0019] Figure 3 It is a structural schematic diagram of a culture plate of the utility model;

[0020] Figure 4 It is a structural schematic diagram of the cover plate of the utility model;

[0021] Figure 5 It is a structural schematic diagram of a gas regulating valve of the utility model;

[0022] Figure 6 It is a schematic structural diagram of a first micro-spray drip irrigation head of the utility model.

[0023] Description of labels:

[0024] 10. Culture plate; 11. Culture tank; 20. Cover plate; 21. Micropore; 30. Gas regulating valve; 31. First micro-spray drip irrigation head; 311. Adjustment cap; 312. Drip irrigation head body; 313. Plug connector; 32. Hose; 33. Needle; 331. Needle plug; 332. Needle stem; 333. Needle tip; 334. Air guide hole; 40. Air pump assembly; 41. Air pump; 42. First air pipe; 43. Second air pipe; 44. Second micro-spray drip irrigation head; 45. Third micro-spray drip irrigation head; 46. Three-way connector; 50. Bracket. DETAILED DESCRIPTION

[0025] The utility model is now further described in conjunction with the accompanying drawings and specific implementation methods.

[0026] The utility model provides a micro device for hatching fish eggs, such as Figures 1 to 6As shown, it includes a culture plate 10 and a cover plate 20 with a movable sealing cover arranged on the culture plate 10, a plurality of gas regulating valves 30 and an air pump assembly 40; the culture plate 10 is provided with a plurality of culture grooves 11, the cover plate 20 is provided with micropores 21 at positions corresponding to the culture grooves 11, the gas regulating valve 30 is provided with an air inlet end and an air outlet end, each micropore 21 is respectively connected to a corresponding air outlet end of a gas regulating valve 30, or, a plurality of micropores 21 are grouped and each group is connected to a air outlet end of a gas regulating valve 30, or, all the micropores 21 are commonly connected to a air outlet end of a gas regulating valve 30, the air inlet end of the gas regulating valve 30 is connected to the air pump assembly 40, and the gas regulating valve 30 regulates the ventilation volume of the culture groove 11 in the micropore 21 by gas diversion.

[0027] The culture plate 10 of the present embodiment is provided with eight culture tanks 11, but it is not limited thereto, and the number of culture tanks 11 can be adjusted according to actual needs. The cover plate 20 movable sealing cover is provided on the culture plate 10 to create a relatively closed space for the culture tank 11. The cover plate 20 is provided with micropores 21 at positions corresponding to the culture tanks 11. In the present embodiment, each micropore 21 is connected to the gas outlet end of the gas regulating valve 30, but it is not limited thereto, and the gas inlet end of the gas regulating valve 30 is connected to the air pump assembly 40, and the air pump assembly 40 ventilates the culture tank 11 in the micropore 21 through the gas regulating valve 30, so as to ensure the water quality without changing the water, improve the success rate of hatching and the survival rate of the young fish, and do not require manual operation, and are easy to use. Multiple culture tanks 11 are also conducive to carrying out batch experiments in a small space. In other embodiments, the culture tanks 11 can be grouped, and each group is only connected to one gas regulating valve 30 for ventilation, or all the micropores 21 are connected to one gas regulating valve 30 for ventilation, which can be easily realized by those skilled in the art.

[0028] like Figure 5 As shown, the gas regulating valve 30 includes a first micro-spray drip irrigation head 31, a needle 33 and a hose 32. The first micro-spray drip irrigation head 31 includes a drip irrigation head body 312 and an adjusting cap 311. A plug connector 313 is provided at one end of the drip irrigation head body 312. A hollow airway (not shown in the figure) is provided inside the plug connector 313. The airway extends to the other end of the drip irrigation head body 312. The adjusting cap 311 is sealingly rotatably arranged at the other end of the drip irrigation head body 312, and is used to adjust the gas flow in the airway in the drip irrigation head body 312. One end of the hose 32 is connected to the needle plug 331 at one end of the needle 33, and the other end of the hose 32 is connected to the plug connector 313 at one end of the drip irrigation head body 312. An air guide hole 334 is opened on the needle stem 332 of the needle 33.

[0029] The gas regulating valve 30 of this embodiment is composed of a first micro-spray drip irrigation head 31, a needle 33 and a hose 32. The first micro-spray drip irrigation head 31 includes a drip irrigation head body 312 and an adjusting cap 311. A plug connector 313 is provided at one end of the drip irrigation head body 312. A pintle 331 at one end of the needle 33 is connected to the plug connector 313 through the hose 32. The hose 32 of this embodiment is made of a polyurethane hose 32, which is light in weight, good in elasticity, not easy to break or deform, and can ensure the sealing between the needle 33 and the drip irrigation head body 312. In other embodiments, a hose 32 made of other materials such as rubber can also be used.

[0030] In this embodiment, a hollow airway is arranged in the plug connector 313 of the drip irrigation head body 312, and the airway extends to the other end of the drip irrigation head body 312. The adjusting cap 311 is sealed and rotatably arranged at the other end of the drip irrigation head body 312, and an air guide hole 334 is opened on the needle stem 332 of the needle 33, so that the gas generated by the air pump assembly 40 can enter the culture tank 11 through the air guide hole 334. When the airway of the drip irrigation head body 312 is completely closed by rotating the adjusting cap 311, the pressure inside the needle 33, the hose 32 and the drip irrigation head body 312 will increase, and the gas generated by the air pump assembly 40 will enter the culture tank 11 through the air guide hole 334. When the airway of the drip irrigation head body 312 is partially closed by rotating the adjusting cap 311, the gas is diverted, so the size of the gas flow entering the culture tank 11 can be adjusted by rotating the adjusting cap 311, which is simple to operate and convenient to use.

[0031] like Figure 5 As shown, in this embodiment, the distance between the air guide hole 334 of the needle stem 332 and the needle plug 331 is between 2 mm and 6 mm, and the shape of the air guide hole 334 is long and strip-shaped, but not limited to this. In other embodiments, the position and shape of the air guide hole 334 can be adjusted according to actual conditions.

[0032] like Figure 1 As shown, the air pump assembly 40 includes an air pump 41, a first air pipe 42, a second micro-spray drip irrigation head 44 and a third micro-spray drip irrigation head 45. The first air pipe 42 is fixedly arranged on the surface of the cover plate 20. The needle tip 333 at the other end of the needle 33 passes through the first air pipe 42 and the micropore 21 in sequence and is located in the culture tank 11. The air guide hole 334 of the needle stem 332 is located in the first air pipe 42. The air inlet at one end of the first air pipe 42 is connected to the air pump 41 through the second micro-spray drip irrigation head 44, and the air outlet at the other end of the first air pipe 42 is connected to the third micro-spray drip irrigation head 45.

[0033] In the present embodiment, the first air pipe 42 is fixedly disposed at the micropore 21 on the surface of the cover plate 20. Since the needle 33 is used as the gas-conducting medium, it is only necessary to pass the needle tip 333 at the other end of the needle 33 through the first air pipe 42 and the micropore 21 in sequence, so that the gas regulating valve 30 is at a vertical angle to the cover plate 20, and the needle 33 is located in the culture tank 11. At this time, the gas-conducting hole 334 of the needle stem 332 is located inside the first air pipe 42, so that the gas generated by the air pump 41 can enter the culture tank 11 through the gas-conducting hole 334 in the first air pipe 42. In the present embodiment, a second micro-spray drip irrigation head 44 and a third micro-spray drip irrigation head 45 are respectively disposed at the air inlet and the air outlet of the first air pipe 42 to adjust the gas pressure inside the first air pipe 42, so as to adjust the gas flow rate entering the culture tank 11. The structure and adjustment process of the second micro-spray drip irrigation head 44 and the third micro-spray drip irrigation head 45 are consistent with those of the aforementioned first micro-spray drip irrigation head 31. For specific structures, refer to Figure 6 , I will not go into details about this.

[0034] like Figure 1 As shown, this embodiment further includes a three-way joint 46, a second air pipe 43 and a hose 32, the air inlet at one end of the first air pipe 42 is connected to the first joint of the three-way joint 46, the second joint of the three-way joint 46 is connected to the air pump 41 through the second air pipe 43, and the third joint of the three-way joint 46 is connected to the plug joint 313 of the drip head body 312 of the second micro-spray drip irrigation head 44 through the hose 32, the structure is simple and easy to implement. In other embodiments, the three-way joint 46 may not be provided, and only one air pipe is used to replace the first air pipe 42 and the second air pipe 43, and the plug joint 313 of the second micro-spray drip irrigation head 44 is directly connected to the air pipe.

[0035] Furthermore, the needle tip 333 of this embodiment is set at a position between 1 mm and 10 mm away from the bottom of the culture tank 11. Since it is necessary to ventilate the incubation liquid or water in the culture tank 11, a certain distance needs to be left between the needle tip 333 and the bottom of the culture tank 11. In other embodiments, it can also be adjusted according to actual conditions.

[0036] like Figure 1 As shown, it also includes at least one bracket 50, which is horizontally mounted on the surface of the cover plate 20. The cross-section of the bracket 50 is U-shaped. The first air pipe 42 is fixedly penetrated in the bracket 50. When one bracket 50 is set, each micropore 21 is correspondingly connected to the outlet end of a gas regulating valve 30. When two or more brackets 50 are set, multiple brackets 50 are horizontally mounted in parallel on the surface of the cover plate 20, and multiple micropores 21 are grouped and each micropore 21 of each group is correspondingly connected to the outlet end of a gas regulating valve 30.

[0037] In the present embodiment, since eight culture troughs 11 are arranged on the culture plate 10, eight gas regulating valves 30 are required accordingly, and therefore two brackets 50 are arranged. The two brackets 50 are fixedly arranged in parallel horizontally at the micropores 21 on the surface of the cover plate 20. A long strip-shaped through hole is arranged at the bottom of the bracket 50 to facilitate the needle tip 333 of the needle 33 to pass through the micropore 21. The first air pipe 42 is fixedly inserted in the bracket 50. By setting the bracket 50, the first air pipe 42 can be effectively prevented from shaking with the airflow, so that the ventilation process is smoother. In other embodiments, the number of the brackets 50 can also be adjusted according to actual needs.

[0038] like Figure 2 and Figure 3 As shown, the shape of the culture trough 11 of this embodiment is circular, but not limited to this, and can also be other shapes such as square. Several culture troughs 11 are evenly distributed on the culture plate 10, and the microholes 21 of the cover plate 20 are opened at positions corresponding to the center of the culture trough 11. The structure is simple and more beautiful.

[0039] like Figure 4 As shown, the diameter of the micropore 21 in this embodiment is between 0.5 mm and 2 mm. The diameter of the micropore 21 needs to be set according to the diameter of the needle tube of the needle 33 to ensure a nearly sealed connection between the needle 33 and the micropore 21.

[0040] Furthermore, a lighting device (not shown) is included, which is arranged above the culture plate 10 to provide light required for hatching. The lighting device of this embodiment is a hatching lamp, but is not limited thereto, and may also be other devices that can provide lighting, such as a desk lamp.

[0041] The use process of the utility model is as follows:

[0042] Select a culture plate 10 with an appropriate number of culture grooves 11, and open microholes 21 at positions corresponding to the culture grooves 11 on the surface of the cover plate 20. The diameter of the microholes 21 matches the diameter of the needle tube of the needle 33. An air guide hole 334 is opened on the needle stem 332 of the needle 33. The distance between the air guide hole 334 and the needle plug 331 of the needle 33 is between 2 mm and 6 mm.

[0043] The bracket 50 is fixed to the micropores 21 on the surface of the cover plate 20, and then the first air pipe 42 and the second air pipe 43 are connected through the two joints of the three-way joint 46, the second air pipe 43 is connected to the air pump 41, and the remaining joint of the three-way joint 46 is connected to the plug joint 313 of the drip head body 312 of the second micro-spray drip irrigation head 44 through the hose 32, and the air outlet of the first air pipe 42 is connected to the third micro-spray drip irrigation head 45.

[0044] The plug connector 313 of the drip head body 312 of the first micro-spray drip head 31 is connected to the needle plug 331 at one end of the needle 33 through the hose 32 to form a gas regulating valve 30. The gas regulating valve 30 passes through the first air pipe 42 and the micropore 21 in sequence through the needle tip 333 at the other end of the needle 33. The distance between the needle tip 333 and the bottom of the culture tank 11 is between 1 mm and 10 mm. The air guide hole 334 on the needle stem 332 is located inside the first air pipe 42.

[0045] Since the plug connector 313 of the drip irrigation head body 312 is provided with a hollow airway (not shown in the figure), the airway extends to the other end of the drip irrigation head body 312, the adjusting cap 311 is sealed and rotatably arranged at the other end of the drip irrigation head body 312, and the needle stem 332 of the needle 33 is provided with an air guide hole 334, the pressure inside the needle 33, the inside of the hose 32 and the inside of the drip irrigation head body 312 will change with the rotation of the adjusting cap 311. When the internal pressure increases, the gas generated by the air pump assembly 40 easily enters the culture tank 11 through the air guide hole 334, and when the internal pressure decreases, the gas generated by the air pump assembly 40 is difficult to enter the culture tank 11 through the air guide hole 334. The gas flow rate entering the culture tank 11 can be adjusted by rotating the adjusting cap 311. In addition, the gas flow rate of the hatching liquid or water body entering the culture tank 11 is in the most ideal state of 60 to 120 bubbles per minute.

[0046] The directional terms mentioned in this specification are defined relative to the structures shown in the drawings. They are relative concepts and may change accordingly according to different locations and different usage conditions. Therefore, these or other directional terms should not be interpreted as restrictive terms.

[0047] The above description is only a preferred embodiment of the present utility model and is not a limitation on the design of the present case. Any equivalent changes made based on the key design of the present case shall fall within the protection scope of the present case.

Claims

1. A micro device for hatching fish eggs, characterized in that: It includes a culture plate and a cover plate with a movable sealing cover arranged on the culture plate, a plurality of gas regulating valves and an air pump assembly; the culture plate is provided with a plurality of culture grooves, the cover plate is provided with micropores at positions corresponding to the culture grooves, the gas regulating valve is provided with an air inlet end and an air outlet end, each micropore is respectively connected to the air outlet end of a gas regulating valve, or a plurality of micropores are grouped and each group is connected to the air outlet end of a gas regulating valve, or all the micropores are connected to the air outlet end of a gas regulating valve, the air inlet end of the gas regulating valve is connected to the air pump assembly, and the gas regulating valve regulates the ventilation volume of the culture groove in the micropore by gas diversion.

2. A micro device for hatching fish eggs as claimed in claim 1, characterized in that: The gas regulating valve comprises a first micro-spray drip irrigation head, a needle and a hose, wherein the first micro-spray drip irrigation head comprises a drip irrigation head body and an adjusting cap, a plug connector is provided at one end of the drip irrigation head body, a hollow airway is provided inside the plug connector, and the airway extends to the other end of the drip irrigation head body, the adjusting cap is sealingly rotatably arranged at the other end of the drip irrigation head body, and is used to adjust the gas flow in the airway in the drip irrigation head body, one end of the hose is connected to the needle plug at one end of the needle, and the other end of the hose is connected to the plug connector at one end of the drip irrigation head body, and an air guide hole is provided on the needle stem of the needle.

3. A micro device for hatching fish eggs as claimed in claim 2, characterized in that: The air guide hole of the needle stem is arranged at a position between 2 mm and 6 mm away from the needle plug.

4. A micro device for hatching fish eggs as claimed in claim 2, characterized in that: The air pump assembly includes an air pump, a first air tube, a second micro-spray drip irrigation head and a third micro-spray drip irrigation head. The first air tube is fixedly arranged on the surface of the cover plate. The needle tip at the other end of the needle passes through the first air tube and the micropore in sequence and is located in the culture tank. The air guide hole of the needle stem is located in the first air tube. The air inlet at one end of the first air tube is connected to the air pump through the second micro-spray drip irrigation head, and the air outlet at the other end of the first air tube is connected to the third micro-spray drip irrigation head.

5. A micro device for hatching fish eggs as claimed in claim 4, characterized in that: It also includes a three-way joint, a second air pipe and a hose, wherein the air inlet at one end of the first air pipe is connected to the first joint of the three-way joint, the second joint of the three-way joint is connected to the air pump through the second air pipe, and the third joint of the three-way joint is connected to the plug joint of the drip irrigation head body of the second micro-spray drip irrigation head through the hose.

6. A micro device for hatching fish eggs as claimed in claim 4, characterized in that: The needle tip is arranged at a position between 1 mm and 10 mm from the bottom of the culture tank.

7. A micro device for hatching fish eggs as claimed in claim 4, characterized in that: It also includes at least one bracket, which is horizontally mounted on the surface of the cover plate, and the cross-section of the bracket is U-shaped. The first air pipe is fixedly inserted into the bracket. When one bracket is set, each micropore is connected to a corresponding outlet end of a gas regulating valve. When two or more brackets are set, multiple brackets are horizontally mounted in parallel on the surface of the cover plate, and multiple micropores are grouped and each micropore in each group is connected to a corresponding outlet end of a gas regulating valve.

8. A micro device for hatching fish eggs as claimed in claim 1, characterized in that: The culture grooves are circular in shape, and a plurality of culture grooves are evenly distributed on the culture plate. The microholes of the cover plate are arranged at positions corresponding to the centers of the culture grooves.

9. A micro device for hatching fish eggs as claimed in claim 1, characterized in that: The diameter of the micropores is between 0.5 mm and 2 mm.

10. A micro device for hatching fish eggs as claimed in claim 1, characterized in that: The invention also comprises a lighting device, which is arranged above the culture plate and is used for providing light required for hatching.

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