A cell culture box with multi-layer structure

Abstract

The utility model discloses a cell culture box with a multi-layer structure, which relates to the technical field of cell culture boxes, and specifically includes a culture box and a control box arranged on one side of the culture box, wherein the culture box and the control box are both arranged on a base, and a controller is arranged in the control box, the base is a hollow shell, and a circulation mechanism for circulating hot air in the culture box is arranged in the base, a temperature sensor is installed on the inner wall of the culture box, and the temperature sensor and the circulation mechanism are respectively connected to the controller through wires; a plurality of shelves arranged at intervals are arranged in the culture box, both sides of the shelves are installed on drawer rails, and the drawer rails are fixed on the inner wall of the culture box, and a plurality of vents are opened on the shelves. The cell culture box with a multi-layer structure drives the screw to push the block by rotating the hand-tightening nut, and pushes it against the arc groove of the culture dish to tighten the culture dish, thereby improving the stability of the culture dish in the placement groove.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture boxes, in particular to a cell culture box with a multi-layer structure. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cells can be transformed from a single cell into a simple single cell or a multi-cell with minimal differentiation through mass culture, which is an indispensable part of cloning technology. The cell culture incubator is a common cell culture device. Researchers place the sampled cell into the cell culture incubator and adjust the environmental parameters in the cell culture incubator to culture the cells.

[0003] When using the existing cell culture incubator, a shelf is set in the cell culture incubator, and then the culture dishes containing culture medium and cells are placed directly on the shelf. However, this method lacks stability for the culture dishes. When taking the culture dishes deeper in the incubator, it is easy to cause other culture dishes to shake due to accidental touch, and even cause the culture dishes to slide off the shelf. Therefore, we propose a multi-layer cell culture incubator to solve the above problems. Utility Model Content

[0004] In view of the deficiency that existing culture dishes are not fixed in the incubator, the utility model provides a cell culture incubator with a multi-layer structure, which has the advantage of fixing the culture dishes placed in the incubator, thereby ensuring the stability of the culture dishes when placed, and solves the problems raised in the above-mentioned background technology.

[0005] To achieve the above purpose, the utility model is implemented by the following technical solutions: a cell culture box with a multi-layer structure, including a culture box, a plurality of shelves arranged at intervals are arranged in the culture box, both sides of the shelves are installed on drawer rails, and the drawer rails are fixed on the inner wall of the culture box, and a plurality of ventilation holes are opened on the shelves;

[0006] Each shelf is provided with placement slots arranged in a matrix, a through hole is opened on one side of the placement slot, and a support tube coaxially arranged with the through hole is installed on the outer side of the placement slot, and a screw is provided inside the support tube;

[0007] A hand-tightening nut is threadedly connected to the screw rod, and an annular rotating seat is coaxially installed on one side of the hand-tightening nut. The rotating seat is rotatably connected to the inner wall of the support tube, and a hemispherical stopper is installed at one end of the screw rod close to the through hole. A sliding groove arranged along the axis direction of the screw rod is opened on the side of the screw rod, and a sliding block is arranged in the sliding groove, and the sliding block is fixedly connected to the inner wall of the support tube.

[0008] It also includes a culture dish for placing culture medium and cell tissues. The culture dish corresponds to the placement groove. A top cover is arranged on the top of the culture dish. An annular arc groove is opened along the bottom edge of the culture dish. The arc groove corresponds to the stop block.

[0009] Preferably, a control box is provided on one side of the incubator, the incubator and the control box are both arranged on a base, and a controller is provided in the control box. The base is a hollow shell, and a circulation mechanism for circulating hot air in the incubator is provided in the base. A temperature sensor is installed on the inner wall of the incubator, and the temperature sensor and the circulation mechanism are respectively connected to the controller through wires.

[0010] Preferably, the stop block is an anti-slip elastic rubber block.

[0011] Preferably, a plurality of spaced mounting seats are installed on the bottom of the outer side of each placement slot, a fixing bolt is installed on the bottom of the mounting seat, and a fixing nut is threadedly connected to the fixing bolt.

[0012] Preferably, a limit plate is installed on one end of the screw rod away from the stop block.

[0013] Preferably, the circulation mechanism includes a circulation fan arranged in the base, the circulation fan is connected to the controller through a wire, an air outlet duct and an air suction duct are installed on the circulation fan, and the other end of the air suction duct is arranged on the bottom of the incubator;

[0014] The air outlet duct passes through the base and is arranged in the inner wall of the incubator, and the end of the air outlet duct away from the circulating fan is connected to an air outlet hood, the air outlet hood is arranged on the top of the incubator, and a heating wire is installed in the air outlet hood, and the heating wire is connected to the controller through a wire.

[0015] Preferably, a sealed door is installed on one side of the incubator, one side of the sealed door is hinged to the incubator and the other side is connected to the incubator via a lock, a window is provided on the sealed door, and a transparent plate is installed in the window;

[0016] A display screen and operation buttons are installed on one side of the control box close to the sealed door, and the display screen and the operation buttons are connected to the controller through wires.

[0017] Compared with the prior art, the utility model discloses a cell culture box with a multi-layer structure, when in use, the screw rod can be controlled to move linearly by turning the hand-tightening nut, so that the stop block is pressed against the arc groove of the culture dish, thereby fastening the culture dish placed in the placement groove and improving the stability of the culture dish in the culture box. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of the utility model;

[0019] Figure 2It is a cross-sectional front view of the utility model;

[0020] Figure 3 This is a schematic diagram of the placement slot structure of the utility model;

[0021] Figure 4 This is a schematic diagram of the screw structure of the utility model;

[0022] Figure 5 This is a schematic diagram of the structure of the culture dish of the utility model.

[0023] In the figure: 1. base; 2. control box; 3. incubator; 4. sealed door; 5. shelf; 6. drawer guide; 7. vent; 8. placement slot; 9. window; 10. through hole; 11. support tube; 12. screw; 13. block; 14. hand nut; 15. slide; 16. slider; 17. limit plate; 18. mounting seat; 19. fixing bolt; 20. fixing nut; 21. culture dish; 22. arc groove; 23. circulation fan; 24. air outlet duct; 25. heating wire; 26. air outlet hood; 27. air suction duct; 28. temperature sensor; 29. ​​rotating seat. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] See also Figures 1 to 5 The utility model provides a technical solution: a multi-layer cell culture box, comprising a culture box 3 and a control box 2 arranged on one side of the culture box 3, the culture box 3 and the control box 2 are both arranged on a base 1, and a controller is arranged in the control box 2, a sealing door 4 is installed on one side of the culture box 3, one side of the sealing door 4 is hinged to the culture box 3 and the other side is connected to the culture box 3 through a lock, and a sealing ring is arranged on the edge of the sealing door 4 to achieve a better sealing effect.

[0026] A window 9 is provided on the sealed door 4, and a transparent plate is installed in the window 9, so that the operator can observe the internal situation of the incubator 3 conveniently. A controller is provided in the control box 2, and a display screen and operation buttons are installed on the side of the control box 2 close to the sealed door 4. The display screen and the operation buttons are connected to the controller through wires. In actual use, the controller is one of a PLC controller, a control host, and a control mainboard. The display screen is a touch screen, and the operation buttons include a start / stop button, a temperature adjustment button, etc.

[0027] like Figure 2 As shown, the base 1 is a hollow shell, and a circulation mechanism for circulating hot air in the incubator 3 is arranged in the base 1, and a temperature sensor 28 is installed on the inner wall of the incubator 3. The temperature sensor 28 and the circulation mechanism are connected to the controller through wires respectively. The temperature sensor 28 monitors the temperature inside the incubator 3 and timely feeds back the temperature situation in the incubator 3 to the controller so that the controller can control the circulation mechanism;

[0028] Among them, Figure 2 As shown, the circulation mechanism includes a circulation fan 23 arranged in the base 1, and the circulation fan 23 is connected to the controller through a wire. An air outlet duct 24 and an air suction duct 27 are installed on the circulation fan 23, and the other end of the air suction duct 27 is arranged on the bottom of the incubator 3. The air outlet duct 24 passes through the base 1 and is arranged in the inner wall of the incubator 3, and the end of the air outlet duct 24 away from the circulation fan 23 is connected to an air outlet hood 26, and the air outlet hood 26 is arranged on the top of the incubator 3, and a heating wire 25 is installed in the air outlet hood 26, and the heating wire 25 is connected to the controller through a wire. The air in the incubator 3 will flow from top to bottom under the action of the circulation fan 23, and then the circulation fan 23 will send the air extracted from the incubator 3 to the air outlet hood 26 and heat it by the heating wire 25, and then enter the incubator 3, so as to realize the hot air circulation operation.

[0029] like Figure 1 As shown, a plurality of shelves 5 arranged at intervals are provided in the incubator 3, both sides of the shelves 5 are mounted on drawer rails 6, and the drawer rails 6 are fixed to the inner wall of the incubator 3, so that the shelves 5 can be moved to the outside of the incubator 3 by pulling, and a plurality of vents 7 are provided on the shelves 5, so that the hot air in the incubator 3 can freely pass through the shelves 5 and flow to the suction duct 27;

[0030] like Figure 1 and Figure 3As shown, each shelf 5 is provided with placement slots 8 arranged in a matrix, a through hole 10 is opened on one side of the placement slot 8, and a support tube 11 coaxially arranged with the through hole 10 is installed on the outer side of the placement slot 8, a screw rod 12 is provided inside the support tube 11, a hand nut 14 is threadedly connected to the screw rod 12, and an annular rotating seat 29 is coaxially installed on one side of the hand nut 14, and the rotating seat 29 is rotatably connected to the inner wall of the support tube 11, and the two are specifically connected through a bearing, and the screw rod 12 A hemispherical stopper 13 is installed at one end close to the through hole 10, a slide groove 15 is provided on the side of the screw rod 12 along the axial direction of the screw rod 12, a slider 16 is provided in the slide groove 15, and the slider 16 is fixedly connected to the inner wall of the support tube 11. The sliding setting of the slider 16 in the slide groove 15 will limit the rotation of the screw rod 12, so that when the hand-tightening nut 14 is rotated, it can drive the screw rod 12 to move linearly in the support tube 11, so as to push the stopper 13 out of the through hole 10;

[0031] The utility model also includes a culture dish 21 for placing culture medium and cell tissues. The culture dish 21 corresponds to the placement groove 8, so that after the culture dish 21 is placed in the placement groove 8, a gap can be formed between the placement groove 8. A top cover is provided on the top of the culture dish 21, and an annular arc groove 22 is opened along the bottom edge of the culture dish 21, and the arc groove 22 corresponds to the stop block 13.

[0032] Specifically, when in use, the operator first pulls out the shelf 5 to the outside of the incubator 3, installs the placement groove 8, then places the culture dish 21 in the placement groove 8, and twists the hand nut 14. At this time, the screw 12 will push the first stop block 13 to approach the culture dish 21 and stop on the arc groove 22 of the culture dish 21. Among them, the stop block 13 is a non-slip elastic rubber block, which increases the stress strength of the stop block 13 on the culture dish 21. At this time, the culture dish 21 will be fastened in the placement groove 8 without shaking or sliding. Then, the shelf 5 is pushed back into the incubator 3, and the sealing door 4 is closed to start cell culture.

[0033] Furthermore, in order to prevent the screw rod 12 from slipping off the hand nut 14 when the hand nut 14 is tightened, a limit plate 17 is installed on the end of the screw rod 12 away from the stop block 13 to limit the movement of the screw rod 12 in the support tube 11.

[0034] Further, such as Figure 1 and Figure 3As shown, a plurality of spaced mounting seats 18 are installed on the bottom of the outer side of each placement slot 8, a fixing bolt 19 is installed on the bottom of the mounting seat 18, and a fixing nut 20 is threadedly connected to the fixing bolt 19. Before placing the placement slot 8 on the shelf 5, first remove the fixing nut 20 on the fixing bolt 19, then place the placement slot 8 on the shelf 5, and pass the fixing bolt 19 through the vent 7 on the shelf 5, and then take the fixing nut 20 from the bottom of the shelf 5 and fix it on the fixing bolt 19, so that the placement slot 8 can be fixed on the shelf 5, and it is also convenient for disassembly and cleaning of the placement slot 8 after use.

[0035] In the description of the present utility model, it should be noted that the orientation or position relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present utility model; the terms "first", "second", and "third" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance. In addition, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances. Furthermore, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not expressly listed, or also includes elements inherent to such process, method, article, or apparatus.

[0036] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A multi-layer cell culture chamber, comprising a culture chamber (3), characterized in that: A plurality of shelves (5) are arranged at intervals in the incubator (3), both sides of the shelves (5) are mounted on drawer rails (6), and the drawer rails (6) are fixed on the inner wall of the incubator (3), and a plurality of ventilation holes (7) are opened on the shelves (5); Each shelf (5) is provided with placement grooves (8) arranged in a matrix, a through hole (10) is opened on one side of the placement groove (8), and a support tube (11) coaxially arranged with the through hole (10) is installed on the outer side of the placement groove (8), and a screw rod (12) is provided inside the support tube (11); A hand-tightening nut (14) is threadedly connected to the screw rod (12), and an annular rotating seat (29) is coaxially installed on one side of the hand-tightening nut (14). The rotating seat (29) is rotatably connected to the inner wall of the support tube (11), and a hemispherical stopper (13) is installed at one end of the screw rod (12) close to the through hole (10). A sliding groove (15) is provided on the side of the screw rod (12) along the axial direction of the screw rod (12), and a sliding block (16) is provided in the sliding groove (15). The sliding block (16) is fixedly connected to the inner wall of the support tube (11); It also includes a culture dish (21) for placing culture medium and cell tissues, the culture dish (21) corresponds to the placement groove (8), a top cover is provided on the top of the culture dish (21), and an annular arc groove (22) is provided along the bottom edge of the culture dish (21), and the arc groove (22) corresponds to the stop block (13).

2. The multi-layer cell culture chamber according to claim 1, characterized in that: A control box (2) is provided on one side of the incubator (3); the incubator (3) and the control box (2) are both arranged on a base (1); a controller is arranged in the control box (2); the base (1) is a hollow shell; a circulation mechanism for circulating hot air in the incubator (3) is arranged in the base (1); a temperature sensor (28) is installed on the inner wall of the incubator (3); and the temperature sensor (28) and the circulation mechanism are respectively connected to the controller via wires.

3. The multi-layer cell culture chamber according to claim 1, characterized in that: The stop block (13) is a non-slip elastic rubber block.

4. The multi-layer cell culture chamber according to claim 1, characterized in that: A plurality of spaced mounting seats (18) are mounted on the bottom of the outer side of each placement slot (8), a fixing bolt (19) is mounted on the bottom of the mounting seat (18), and a fixing nut (20) is threadedly connected to the fixing bolt (19).

5. The multi-layer cell culture chamber according to claim 1, characterized in that: A limit plate (17) is installed on one end of the screw rod (12) away from the stop block (13).

6. The multi-layer cell culture chamber according to claim 2, characterized in that: The circulation mechanism comprises a circulation fan (23) arranged in the base (1), the circulation fan (23) being connected to the controller via a wire, an air outlet duct (24) and an air suction duct (27) being installed on the circulation fan (23), and the other end of the air suction duct (27) being arranged on the bottom of the incubator (3); An air outlet duct (24) passes through the base (1) and is arranged in the inner wall of the incubator (3), and an end of the air outlet duct (24) away from the circulating fan (23) is connected to an air outlet hood (26), the air outlet hood (26) is arranged on the top of the incubator (3), and a heating wire (25) is installed in the air outlet hood (26), and the heating wire (25) is connected to the controller through a wire.

7. The multi-layer cell culture chamber according to claim 6, characterized in that: A sealed door (4) is installed on one side of the incubator (3); one side of the sealed door (4) is hinged to the incubator (3) and the other side is connected to the incubator (3) via a lock; a window (9) is provided on the sealed door (4), and a transparent plate is installed in the window (9); A display screen and operating buttons are installed on one side of the control box (2) close to the sealing door (4), and the display screen and the operating buttons are connected to the controller via wires.

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