A modular, multi-layered cell factory

Abstract

The utility model discloses a modular multi-layer cell factory, including an observation layer, a vent, a chute, a first magnetic block, a second magnetic block, an auxiliary plate, a limit plate, a combined culture piece, a fixed plate, a three-way valve, a connecting tube, an infusion port, a rubber plug, and a hose. The utility model drives the limit plate to move in the chute through the auxiliary plate, and cooperates with the use of the first magnetic block, the second magnetic block, and the third magnetic block to achieve stable placement of the limit plate. When the auxiliary plate is magnetically connected to the first magnetic block, the limit plate is completely embedded in the interior of the chute to achieve separation of the observation layer from the combined culture piece. When the auxiliary plate and the limit plate are magnetically connected to the second magnetic block and the third magnetic block respectively, the limit plate is completely embedded in the bottom end of the combined culture piece to achieve a snap-on combination of the observation layer and the combined culture piece. The connection structure is simple and efficient, and is convenient for fast and convenient combined installation and disassembly, thereby improving the flexibility of the use of the multi-layer cell factory.

Description

Technical Field

[0001] The utility model relates to the field related to cell factories, in particular to a modular multi-layer cell factory. Background Art

[0002] A conventional multi-layer cell factory refers to a multi-layer cell culture container stacked vertically to form an integral structure with uniform liquid addition and discharge ports. The product with the most layers on the market has up to 40 layers. The cultured cells grow adherently to the bottom surface of each layer, which greatly improves the cell culture density and operating efficiency per unit volume. During the culture process, the liquid addition and discharge ports can also be connected to a special breathable cover to supplement the carbon dioxide concentration in the culture container, which has a high application value in the large-scale culture of adherent cells.

[0003] The Chinese patent with patent number CN202221040096.0 discloses "A Modular Multilayer Cell Factory", which independently sets ventilation holes on the top layer, observation layer, and conventional layer, and the layers are detachably connected by connecting plates, so that the number and position of the observation layer and conventional layer can be adjusted easily. In addition, the amount of culture medium used in each layer is equal, the culture conditions are highly uniform, and the observation layer can be easily removed and placed under a microscope for observation;

[0004] This structure has certain advantages, but still has some shortcomings:

[0005] The top layer, observation layer and conventional layer are connected by connecting plates, bolts and nuts. This connection method still requires the use of external tools for installation and disassembly, which is not conducive to the simple construction of the multi-layer cell factory and the rapid transfer of the observation layer to achieve the purpose of observation under a microscope. The structure needs to be improved to make the connection between the top layer, observation layer and conventional layer simpler and faster, and improve the efficiency of installation and observation. Utility Model Content

[0006] Therefore, in order to solve the above-mentioned deficiencies, the utility model provides a modular multi-layer cell factory.

[0007] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a modular multi-layer cell factory, including an observation layer, a vent, a slide, a first magnetic block, a second magnetic block, an auxiliary plate, a limit plate, a combined culture piece, a fixed plate, a three-way valve, a connecting tube, an infusion port, a rubber stopper, and a hose;

[0008] A vent is provided at the front end of the observation layer, a slide is provided at the top end of the observation layer, a first magnetic block is fixed to the bottom side of the front end of the slide, and a second magnetic block is fixed to the side of the front end of the slide;

[0009] The outer sides of the auxiliary plate can be magnetically connected to the first magnetic block and the second magnetic block respectively, and a limiting plate is fixed to the rear end of the auxiliary plate, and the limiting plate moves inside the slide slot;

[0010] The limiting plate is clamped with the bottom end of the combined culture component, and a fixing plate is fixed to the right end of the combined culture component;

[0011] The three-way valve is installed at the right end of the combined culture component and is connected to the interior of the combined culture component;

[0012] The top end of the three-way valve is connected to the connecting pipe, the outer side of the connecting pipe is in contact with the fixing plate, and the front end of the connecting pipe is provided with an infusion port;

[0013] A rubber stopper is embedded in the front end of the infusion port.

[0014] Preferably, the combined culture component includes a base, a partition, a snap-in groove, and a third magnetic block. A partition is fixed inside the base, snap-in grooves are provided at both upper and lower ends of the base, a third magnetic block is fixed at the inner right end of the snap-in groove, and the third magnetic block is magnetically connected to the limiting plate.

[0015] It is further preferred that the three-way valves are provided with multiple groups, and every two groups of three-way valves are connected by hoses, so as to facilitate the combined installation of multiple groups of combined culture pieces and observation layers, and a three-way valve is also installed on the right end of the observation layer, wherein the fixed plate and the connecting pipe are installed in the top-layer combined culture piece of the multi-layer cell factory device.

[0016] It is further preferred that the slide groove, the first magnetic block, the second magnetic block, the auxiliary plate, and the limit plate constitute an active group A, and the active group A is provided with multiple groups, which is convenient for assembling the observation layer with the upper and lower groups of combined culture pieces, and facilitates the flexible construction and use of the multi-layer cell factory.

[0017] It is further preferred that the opening of the snap-fit ​​groove is inclined outward to facilitate the entry of the limiting plate and achieve rapid alignment and combined installation.

[0018] In a further embodiment, the cross section of the limiting plate is concave, and the limiting plates on the same horizontal line are arranged bilaterally symmetrically, so as to facilitate the stable connection between the observation layer and the combined culture piece.

[0019] It is further preferred that the front end of the auxiliary plate is arranged in a stripe shape to increase friction and facilitate the user to slide the auxiliary plate.

[0020] It is further preferred that the auxiliary plate and the limit plate are both made of stainless steel, which has high strength and is not prone to bending, and is easy to be magnetically connected to the magnetic block to achieve stable clamping and resetting of the limit plate.

[0021] Beneficial effects of the utility model:

[0022] The utility model drives the limit plate to move in the slide groove by the auxiliary plate, and cooperates with the use of the first magnetic block, the second magnetic block and the third magnetic block to achieve stable placement of the limit plate. When the auxiliary plate is magnetically connected to the first magnetic block, the limit plate is completely embedded in the interior of the slide groove to achieve separation of the observation layer and the combined culture piece. When the auxiliary plate and the limit plate are magnetically connected to the second magnetic block and the third magnetic block respectively, the limit plate is completely embedded in the bottom end of the combined culture piece to achieve a snap-on combination of the observation layer and the combined culture piece. The connection structure is simple and efficient, and is convenient for fast and convenient combined installation and disassembly, thereby improving the flexibility of use of the multi-layer cell factory.

[0023] The utility model provides snap-in grooves at the upper and lower ends of the base to facilitate snap-in connection between the base and the observation layer, thereby achieving flexible assembly and further flexible adjustment of the number of layers of the multi-layer cell factory, thereby improving the flexibility of constructing the multi-layer cell factory. The opening of the snap-in groove is inclined to facilitate rapid alignment and embedding of the base and the observation layer, and the provision of the third magnetic block enhances the stability of the limit plate during snap-in connection. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0025] Figure 2 It is a schematic diagram of the cross-sectional structure of the utility model;

[0026] Figure 3 This is a schematic diagram of the three-dimensional structure of the observation layer of the utility model;

[0027] Figure 4 It is a schematic diagram of the three-dimensional structure of the auxiliary plate, the limit plate, the first magnetic block and the second magnetic block of the utility model;

[0028] Figure 5 It is a schematic diagram of the internal structure of the combined culture piece of the utility model;

[0029] Figure 6 It is a detailed enlarged structural schematic diagram of the combined culture piece of the utility model.

[0030] Among them: observation layer-1, air vent-2, slide groove-3, first magnetic block-4, second magnetic block-5, auxiliary plate-6, limit plate-7, combined culture piece-8, fixed plate-9, three-way valve-10, connecting tube-11, infusion port-12, rubber stopper-13, hose-14, base-81, partition-82, card slot-83, third magnetic block-84. DETAILED DESCRIPTION

[0031] In order to further explain the technical solution of the present utility model, it is described in detail through specific embodiments below.

[0032] See also Figure 1-4 The utility model provides a modular multi-layer cell factory, comprising an observation layer 1, a vent 2, a chute 3, a first magnetic block 4, a second magnetic block 5, an auxiliary plate 6, a limit plate 7, a combined culture piece 8, a fixed plate 9, a three-way valve 10, a connecting pipe 11, an infusion port 12, a rubber plug 13, and a hose 14; the front end of the observation layer 1 is provided with a vent 2, the top of the observation layer 1 is provided with a chute 3, the front bottom side of the chute 3 is fixed with a first magnetic block 4, and the front side thereof is fixed with a second magnetic block 5; the outer sides of the auxiliary plates 6 can be respectively connected with the first magnetic The auxiliary plate 6 is magnetically connected to the second magnetic block 4 and the second magnetic block 5, the rear end of the auxiliary plate 6 is fixed with a limiting plate 7, and the limiting plate 7 moves inside the slide groove 3; the limiting plate 7 is snap-fitted with the bottom end of the combined culture piece 8, and the right end of the combined culture piece 8 is fixed with a fixing plate 9; the three-way valve 10 is installed at the right end of the combined culture piece 8, and it is connected with the interior of the combined culture piece 8; the top end of the three-way valve 10 is connected with the connecting pipe 11, the outer side of the connecting pipe 11 is in contact with the fixing plate 9, and the front end thereof is provided with an infusion port 12; the front end of the infusion port 12 is embedded with a rubber plug 13.

[0033] In this embodiment, multiple groups of three-way valves 10 are provided, and every two groups of three-way valves 10 are connected by means of hoses 14, so as to realize the combination and closed installation of multiple groups of combined culture pieces 8 and observation layers 1, and a three-way valve 10 is also installed at the right end of the observation layer 1, wherein the fixed plate 9 and the connecting pipe 11 are both installed in the topmost combined culture piece 8 of the multi-layer cell factory device.

[0034] In this embodiment, the slide groove 3, the first magnetic block 4, the second magnetic block 5, the auxiliary plate 6, and the limit plate 7 constitute an active group A. The active group A is provided with multiple groups, which is convenient for assembling the observation layer 1 with the upper and lower groups of combined culture pieces 8, and is convenient for the flexible construction and use of the multi-layer cell factory.

[0035] In this embodiment, the cross-section of the limiting plate 7 is "concave" shaped, and the limiting plates 7 on the same horizontal line are symmetrically arranged on the left and right, which is convenient for achieving a stable connection between the observation layer 1 and the combined culture piece 8. Two groups of symmetrical "concave" shaped limiting plates 7 are used to mutually inhibit the left and right movement of the combined culture piece 8, thereby ensuring a stable connection between the observation layer 1 and the combined culture piece 8.

[0036] In this embodiment, the front end of the auxiliary plate 6 is arranged in a stripe shape to increase friction and facilitate the user to slide the auxiliary plate 6.

[0037] An air filter membrane is fixed in the middle of the air vent 2 to prevent external harmful air substances from affecting cell culture.

[0038] See also Figure 5-6The utility model provides a modular multi-layer cell factory, wherein the combined culture component 8 comprises a substrate 81, a partition 82, a snap-in groove 83, and a third magnetic block 84. The partition 82 is fixed inside the substrate 81, and snap-in grooves 83 are provided at both upper and lower ends of the substrate 81. The third magnetic block 84 is fixed at the right end inside the snap-in groove 83, and the third magnetic block 84 is magnetically connected to the limiting plate 7.

[0039] In this embodiment, the opening of the snap-in groove 83 is inclined outward, which is convenient for the entry of the limiting plate 7, so as to realize quick alignment and combined installation, and by setting the partition 82, the interior of the combined culture piece 8 is divided into three groups of culture chambers. By installing the observation layer 1 in two groups of combined culture pieces 8, it is equivalent to selecting one group out of the seven groups of culture chambers for observation, so as to realize equal observation of the cell factory, facilitate regular extraction and inspection of cell culture quality, and thus more accurately know the cell culture quality in the culture fluid of this group.

[0040] The front end of the combined culture piece 8 is also provided with a ventilation opening 2 .

[0041] See also Figure 1-Figure 6 When in use, place the substrate 81 on the top of the observation layer 1, align their edges, and the air vents 2 are uniformly facing forward, then move the auxiliary plate 6 upward to separate the auxiliary plate 6 from the first magnetic block 4, and at the same time, the auxiliary plate 6 drives the limiting plate 7 to move upward, so that the limiting plate 7 moves up and is embedded in the clamping groove 83, and then move the auxiliary plate 6 to the left to make the auxiliary plate 6 and the second magnetic block 5, and the limiting plate 7 and the third magnetic block 84 magnetically connected, so that the observation layer 1 and the substrate 81 are clamped, repeat the operation, fully combine and install the observation layer 1 and the two groups of substrates 81, and use the hose 14 to connect the observation layer 1 and the three-way valve 10 in the substrate 81, so that the observation layer 1 is connected to the infusion of the substrate 81, which is convenient for the subsequent unified infusion, and then;

[0042] The cell factory is turned over 90 degrees, so that the infusion port 12 opens upward, and the rubber stopper 13 is pulled out, and the cell culture fluid is poured in. The cell culture fluid enters the observation layer 1 and the culture chamber inside the matrix 81 in equal amounts along the connecting tube 11, the three-way valve 10, and the hose 14, wherein, when the culture fluid is filled, the three-way valve 10 and the hose 14 are both placed horizontally, so that the culture fluid entering the culture chamber is nearly equal, and the culture conditions are unified. Then, the multi-layer cell factory is reset, and the rubber stopper 13 is reset, and the device is placed in a cell culture box for culture;

[0043] When cells need to be observed during the culture process, the auxiliary plate 6 drives the limiting plate 7 to completely return to the slide groove 3, so that the observation layer 1 is separated from the base 81, and then the observation layer 1 is rotated to be placed under a microscope for observation. During the rotation process, the soft characteristics of the hose 14 can support the rotation of the observation layer, so that the observation layer 1 can be rotated out for observation.

[0044] After the cell culture is completed, the cell culture solution is poured out into the liquid collection container through the infusion port 12 .

[0045] The control method of the present invention is to control by manually starting and closing the switch. The wiring diagram of the power element and the provision of power supply are common knowledge in the field, and the present invention is mainly used to protect mechanical devices, so the present invention will not explain the control method and wiring arrangement in detail.

[0046] The control method of the present invention is automatic control through a controller. The control circuit of the controller can be realized by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will no longer explain the control method and circuit connection in detail.

[0047] The above description is only a preferred example of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions described in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A modular multi-layer cell factory, characterized in that: It comprises an observation layer (1), a vent (2), a slide groove (3), a first magnetic block (4), a second magnetic block (5), an auxiliary plate (6), a limit plate (7), a combined culture piece (8), a fixing plate (9), a three-way valve (10), a connecting tube (11), an infusion port (12), a rubber stopper (13), and a hose (14); The front end of the observation layer (1) is provided with a vent (2), the top end of the observation layer (1) is provided with a slide groove (3), the front end bottom side of the slide groove (3) is fixed with a first magnetic block (4), and the front end side thereof is fixed with a second magnetic block (5); The outer sides of the auxiliary plate (6) can be magnetically connected to the first magnetic block (4) and the second magnetic block (5) respectively, and a limiting plate (7) is fixed to the rear end of the auxiliary plate (6), and the limiting plate (7) moves inside the slide groove (3); The limiting plate (7) is snap-connected with the bottom end of the combined culture member (8), and a fixing plate (9) is fixed to the right end of the combined culture member (8); The three-way valve (10) is installed at the right end of the combined culture component (8), and is connected to the interior of the combined culture component (8); The top end of the three-way valve (10) is connected to the connecting pipe (11), the outer side of the connecting pipe (11) is in contact with the fixing plate (9), and the front end of the connecting pipe (11) is provided with an infusion port (12); A rubber stopper (13) is embedded in the front end of the infusion port (12).

2. A modular multi-layer cell factory according to claim 1, characterized in that: The combined culture element (8) comprises a base (81), a partition (82), a snap-in groove (83), and a third magnetic block (84); the base (81) has a partition (82) fixed therein; the base (81) has snap-in grooves (83) at both upper and lower ends; the third magnetic block (84) is fixed at the right end of the snap-in groove (83); and the third magnetic block (84) is magnetically connected to the limiting plate (7).

3. A modular multi-layer cell factory according to claim 1, characterized in that: The three-way valves (10) are provided in multiple groups, and every two groups of three-way valves (10) are connected by means of a hose (14).

4. A modular multi-layer cell factory according to claim 1, characterized in that: The slide groove (3), the first magnetic block (4), the second magnetic block (5), the auxiliary plate (6), and the limit plate (7) form an active group (A), and the active group (A) is provided with a plurality of groups.

5. A modular multi-layer cell factory according to claim 2, characterized in that: The opening of the clamping groove (83) is inclined outwards.

6. A modular multi-layer cell factory according to claim 1, characterized in that: The cross section of the limiting plate (7) is concave, and the limiting plates (7) on the same horizontal line are arranged in a bilaterally symmetrical manner.

7. A modular multi-layer cell factory according to claim 2, characterized in that: The front end of the auxiliary plate (6) is arranged in a stripe shape.

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