Cell culture device

Abstract

The utility model relates to the technical field of cell culture, and discloses a cell culture device which comprises a culture dish, a culture solution storage container, a waste liquid collection container, a culture solution conveying pipe, a waste liquid recovery pipe and a liquid pump, the input end of the culture solution conveying pipe extends into the culture solution storage container, the output end of the culture solution conveying pipe extends into the culture dish, the input end of the waste liquid recovery pipe extends into the culture dish, and the output end of the waste liquid recovery pipe extends into the waste liquid collection container. The liquid pump rotates to convey the culture solution in the cell vessel into the waste liquid collecting container through the waste liquid recycling pipe, and then the liquid pump reversely rotates to convey the culture solution in the culture solution storage container into the culture dish through the culture solution conveying pipe, so that the culture solution in the culture dish is replaced, the waste culture solution does not need to be manually sucked, the operation is simple, and the cost is low. The culture solution is not easy to pollute.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture device. Background Art

[0002] Cell culture requires regular replacement of culture medium to provide nutrients and growth factors required for cell growth. Currently, culture medium replacement is done by manually aspirating the old culture medium and adding fresh culture medium, which is labor-intensive and prone to contamination. Utility Model Content

[0003] The purpose of the utility model is to provide a cell culture device which can automatically change liquid.

[0004] In order to achieve the above-mentioned object, the utility model provides a cell culture device, including a culture dish, a culture fluid storage container, a waste fluid collection container, a culture fluid delivery pipe, a waste fluid recovery pipe and a liquid pump.

[0005] The culture solution delivery pipe and the waste liquid recovery pipe are both connected to the liquid pump.

[0006] The input end of the culture solution delivery tube extends into the culture solution storage container, and the output end extends into the culture dish.

[0007] The input end of the waste liquid recovery pipe extends into the culture dish, and the output end extends into the waste liquid collection container.

[0008] Among them, the outer peripheral wall of the output end of the culture fluid delivery tube is attached to the inner wall of the culture dish, and the output end of the culture fluid delivery tube is facing the inner bottom wall of the culture dish, and the outer peripheral wall of the input end of the waste liquid recovery tube is attached to the inner bottom wall of the culture dish, and the input end of the waste liquid recovery tube is facing the inner wall of the culture dish.

[0009] In a specific embodiment of the present invention, the output end of the culture solution delivery tube and the input end of the waste liquid recovery tube are respectively located on two opposite sides of the center of the culture dish.

[0010] In a specific embodiment of the present invention, the input end of the culture solution delivery tube extends to the bottom of the inner cavity of the culture solution storage container, and there is a distance between the input end of the culture solution delivery tube and the bottom surface of the inner cavity of the culture solution storage container.

[0011] In a specific embodiment of the utility model, the output end of the waste liquid recovery pipe is located at the upper part of the inner cavity of the waste liquid collection container.

[0012] In a specific embodiment of the utility model, the temperature control device and the heating device are also included. The temperature control device is used to keep the culture solution storage container at a first preset temperature, and the heating device is used to keep the culture solution delivery pipe at a second preset temperature.

[0013] Wherein, the first preset temperature is lower than the second preset temperature.

[0014] In a specific embodiment of the utility model, the temperature control device includes a trough body and a temperature control component, the temperature control component is in contact with the trough body, the culture fluid storage container is arranged in the trough body, and the temperature control component is used to make the culture fluid storage container at a first preset temperature.

[0015] In a specific embodiment of the utility model, the heating device comprises a heating wire, and the heating wire is used to make the culture liquid delivery pipe part at a second preset temperature.

[0016] In a specific embodiment of the utility model, it also includes a cell culture box, which has a culture chamber, and the culture dish, culture fluid storage container, waste liquid collection container, culture fluid delivery pipe, waste liquid recovery pipe and liquid pump are all arranged in the culture chamber.

[0017] In a specific embodiment of the utility model, it also includes a microscope, the microscope includes a stage, and the culture dish is arranged on the stage.

[0018] The cell culture device of the present utility model has the following advantages compared with the prior art:

[0019] In the cell culture device of the embodiment of the utility model, in actual application, culture fluid is stored in the culture fluid storage container. When the culture fluid of the culture dish needs to be replaced, the liquid pump rotates to transport the culture fluid in the cell dish to the waste liquid collection container through the waste liquid recovery pipe, and then the liquid pump rotates in the opposite direction to transport the culture fluid in the culture fluid storage container to the culture dish through the culture fluid delivery pipe, thereby completing the replacement of the culture fluid in the culture dish, without the need to manually suck out the waste culture fluid, the operation is simple, and the culture fluid is not easily contaminated; in addition, the outer peripheral wall of the output end of the culture fluid delivery pipe is attached to the inner wall of the culture dish, and the culture fluid output end faces the inner bottom wall of the culture dish, thereby, when the culture fluid delivery pipe outputs the culture fluid, the culture fluid can flow down along the inner wall of the culture dish, and there will be no splashing of the culture fluid; and the outer peripheral wall of the input end of the waste liquid recovery pipe is attached to the inner bottom wall of the culture dish, and the input end of the waste liquid recovery pipe faces the inner wall of the culture dish, thereby, the waste liquid recovery pipe can absorb the culture fluid more fully, which is conducive to complete replacement of the culture fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a three-dimensional structural diagram of a cell culture device according to an embodiment of the utility model;

[0021] Figure 2 is a schematic diagram of a cell culture device according to an embodiment of the utility model;

[0022] Figure 3 It is a structural diagram of the cooperation of a culture dish, a culture liquid delivery pipe and a waste liquid recovery pipe in an embodiment of the utility model.

[0023] In the figure, 1. culture dish; 2. culture medium storage container; 3. waste liquid collection container; 4. culture medium delivery pipe; 5. waste liquid recovery pipe; 6. liquid pump; 7. temperature control device; 8. heating device; 9. cell culture box; 10. microscope. DETAILED DESCRIPTION

[0024] The following is a further detailed description of the specific implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0025] like Figure 1-3 As shown, a cell culture device of an embodiment of the utility model includes a culture dish 1, a culture liquid storage container 2, a waste liquid collection container 3, a culture liquid delivery pipe 4, a waste liquid recovery pipe 5 and a liquid pump 6, wherein the culture liquid delivery pipe 4 and the waste liquid recovery pipe 5 are both connected to the liquid pump 6, the input end of the culture liquid delivery pipe 4 extends into the culture liquid storage container 2, and the output end extends into the culture dish 1, the input end of the waste liquid recovery pipe 5 extends into the culture dish 1, and the output end extends into the waste liquid collection container 3, wherein the outer peripheral wall of the output end of the culture liquid delivery pipe 4 is attached to the inner wall of the culture dish 1, and the output end of the culture liquid delivery pipe 4 faces the inner bottom wall of the culture dish 1, and the outer peripheral wall of the input end of the waste liquid recovery pipe 5 is attached to the inner bottom wall of the culture dish 1, and the input end of the waste liquid recovery pipe 5 faces the inner wall of the culture dish 1.

[0026] In practical applications, the culture medium storage container 2 stores the culture medium. When the culture dish 1 needs to replace the culture medium, the liquid pump 6 rotates to transport the culture medium in the cell dish through the waste liquid recovery pipe 5 to the waste liquid collection container 3, and then the liquid pump 6 rotates in the opposite direction to transport the culture medium in the culture medium storage container 2 to the culture dish 1 through the culture medium delivery pipe 4, thereby completing the replacement of the culture medium in the culture dish 1, without the need to manually absorb the waste culture medium, the operation is simple, and the culture medium is not easily contaminated; in addition, the outer peripheral wall of the output end of the culture medium delivery pipe 4 is attached to the inner wall of the culture dish 1, and the culture medium output end faces the inner bottom wall of the culture dish 1, thereby, when the culture medium delivery pipe 4 outputs the culture medium, the culture medium can flow down along the inner wall of the culture dish 1, and there will be no splashing of the culture medium; and the outer peripheral wall of the input end of the waste liquid recovery pipe 5 is attached to the inner bottom wall of the culture dish 1, and the input end of the waste liquid recovery pipe 5 faces the inner wall of the culture dish 1, thereby, the waste liquid recovery pipe 5 can absorb the culture medium more fully, which is conducive to complete replacement of the culture medium.

[0027] As a specific implementation of this embodiment, the liquid pump 6 is a dual-channel peristaltic pump, each channel is controlled separately, the culture medium delivery tube 4 is connected to one of the channels, and the waste liquid recovery tube 5 is connected to the other channel. The liquid pump 6 of this structure is the prior art, and this embodiment will not go into too much detail. The peristaltic pump adopts an intelligent, efficient, low-noise, and durable pump to ensure stable liquid delivery.

[0028] In this embodiment, the output end of the culture solution delivery tube 4 and the input end of the waste solution recovery tube 5 are respectively located on two opposite sides of the center of the culture dish 1, and there is a certain distance between the two for easy assembly.

[0029] The input end of the culture medium delivery tube 4 extends to the bottom of the inner cavity of the culture medium storage container 2, and there is a distance between the input end of the culture medium delivery tube 4 and the bottom surface of the inner cavity of the culture medium storage container 2. Therefore, when replacing the culture medium in the culture dish 1, the culture medium delivery tube 4 can transport the culture medium at the bottom of the inner cavity of the culture medium storage container 2 to the culture dish 1, thereby fully utilizing the culture medium in the culture medium storage container 2 and extending the replenishment cycle of the culture medium in the culture medium storage container 2.

[0030] The output end of the waste liquid recovery pipe 5 is located at the upper part of the inner cavity of the waste liquid collection container 3, thereby making full use of the accommodation space of the waste liquid collection container 3 and extending the cleaning cycle of the waste liquid in the waste liquid collection container 3.

[0031] Furthermore, the cell culture device also includes a temperature control device 7 and a heating device 8, the temperature control device 7 is used to make the culture fluid storage container 2 at a first preset temperature, and the heating device 8 is used to make the culture fluid delivery pipe 4 part at a second preset temperature, wherein the first preset temperature is lower than the second preset temperature; in practical applications, the culture fluid has a suitable storage temperature, and the cell culture has a suitable temperature. As a specific implementation of this embodiment, the first preset temperature is 0-10°C. At this temperature, the storage time of the culture fluid is relatively long, and the second preset temperature is 30-37°C, which makes the culture fluid delivered to the culture dish 1 have a suitable temperature, thereby ensuring the normal progress of the cell culture work and improving the success rate and efficiency of the cell culture.

[0032] Specifically, the temperature control device 7 includes a tank body and a temperature control component, the temperature control component is in contact with the tank body, and the cell fluid storage container is arranged in the tank body. The temperature control component is used to make the culture fluid storage container 2 at a first preset temperature. It has a simple structure and can effectively make the culture fluid storage container 2 at the first preset temperature. The temperature control component can be a semiconductor refrigeration plate, which cooperates with the temperature sensor to control the environment inside the tank body to be at the first preset temperature.

[0033] The heating device 8 includes a heating wire, which is used to keep part of the culture fluid delivery pipe 4 at a second preset temperature. Similarly, the heating wire cooperates with another temperature sensor to control the temperature at the second preset temperature. It has a simple structure and is easy to control. The culture fluid delivery pipe 4 is made of heat-resistant and corrosion-resistant material. In addition, the utility model does not limit the arrangement of the heating wire, which can be arranged in a serpentine or ring shape, and the heating device 8 can also include an outer shell, and the heating wire is arranged in the outer shell.

[0034] In this embodiment, the cell culture device also includes a cell culture box 9, which has a culture cavity, and the culture dish 1, the culture fluid storage container 2, the waste liquid collection container 3, the culture fluid delivery pipe 4, the waste liquid recovery pipe 5, the liquid pump 6, the temperature control device 7 and the heating device 8 are all arranged in the culture cavity, wherein the cell culture box 9 is a prior art, which has functions such as temperature and humidity control, and can control the temperature and humidity of the environment in the culture cavity, etc., and can provide a suitable environment for cell culture, while the temperature control device 7 and the heating device 8 only control the local position of the culture cavity, and the temperature and humidity control function of the cell culture box 9 will not affect the operation of the temperature control device 7 and the heating device 8.

[0035] Furthermore, the cell culture device also includes a microscope 10, which includes a stage, and the culture dish 1 is arranged on the stage, wherein the microscope 10 is a fully automatic microscope, which is a prior art and is not limited to this in this embodiment. Preferably, the microscope adopts a fully automatic microscope with temperature and humidity resistance characteristics, so that it can be placed in the culture chamber of the cell culture box to continuously monitor the cell growth. In addition, an automatic observation program can also be set to automatically take images to facilitate the observation of cell growth.

[0036] In summary, in practical applications, the liquid pump 6 will automatically transport the waste liquid in the culture dish 1 to the waste liquid collection container 3 every day or every few days. After the waste liquid is sucked away, the set volume of fresh culture liquid will be automatically transported from the culture liquid storage container 2 to the cell culture vessel through the culture liquid delivery pipe 4 immediately (within 5 seconds), and during the transportation process, the heating device 8 makes the culture liquid delivery pipe 4 partly at a second preset temperature. Through this system, manual operation is no longer required during the cell culture process (several days or even months), and long-term, fully automatic, and low-cost cell culture fluid replacement can be achieved, which improves the stability of cell culture and fluid replacement stability, reduces the possibility of experimental personnel's operation contamination, and also reduces the uncertainty of manual fluid replacement operation errors on cell growth, reduces the working hours of cell culture personnel, and reduces the cost of cell culture personnel in the laboratory.

[0037] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and substitutions can be made without departing from the technical principles of the present invention. These improvements and substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A cell culture device, characterized in that: The invention comprises a culture dish (1), a culture liquid storage container (2), a waste liquid collection container (3), a culture liquid delivery pipe (4), a waste liquid recovery pipe (5) and a liquid pump (6). The culture liquid delivery pipe (4) and the waste liquid recovery pipe (5) are both connected to the liquid pump (6). The input end of the culture solution delivery pipe (4) extends into the culture solution storage container (2), and the output end extends into the culture dish (1). The input end of the waste liquid recovery pipe (5) extends into the culture dish (1), and the output end extends into the waste liquid collection container (3). The outer peripheral wall of the output end of the culture liquid delivery pipe (4) is in contact with the inner wall of the culture dish (1), and the output end of the culture liquid delivery pipe (4) faces the inner bottom wall of the culture dish (1); the outer peripheral wall of the input end of the waste liquid recovery pipe (5) is in contact with the inner bottom wall of the culture dish (1), and the input end of the waste liquid recovery pipe (5) faces the inner wall of the culture dish (1).

2. The cell culture device according to claim 1, characterized in that: The output end of the culture liquid delivery pipe (4) and the input end of the waste liquid recovery pipe (5) are respectively located on two opposite sides of the center of the culture dish (1).

3. The cell culture device according to claim 1, characterized in that: The input end of the culture solution delivery tube (4) extends to the bottom of the inner cavity of the culture solution storage container (2), and there is a distance between the input end of the culture solution delivery tube (4) and the bottom surface of the inner cavity of the culture solution storage container (2).

4. The cell culture device according to claim 1, characterized in that: The output end of the waste liquid recovery pipe (5) is located at the upper part of the inner cavity of the waste liquid collection container (3).

5. The cell culture device according to any one of claims 1 to 4, characterized in that: It also comprises a temperature control device (7) and a heating device (8), wherein the temperature control device (7) is used to keep the culture solution storage container (2) at a first preset temperature, and the heating device (8) is used to keep the culture solution delivery pipe (4) at a second preset temperature. Wherein, the first preset temperature is lower than the second preset temperature.

6. The cell culture device according to claim 5, characterized in that: The temperature control device (7) comprises a tank body and a temperature control component, wherein the temperature control component is in contact with the tank body, the culture fluid storage container (2) is arranged in the tank body, and the temperature control component is used to keep the culture fluid storage container (2) at a first preset temperature.

7. The cell culture device according to claim 5, characterized in that: The heating device (8) comprises a heating wire, and the heating wire is used to keep the culture liquid conveying pipe (4) part at a second preset temperature.

8. The cell culture device according to claim 1, characterized in that: It also comprises a cell culture box (9), wherein the cell culture box (9) has a culture chamber, and the culture dish (1), the culture fluid storage container (2), the waste fluid collection container (3), the culture fluid delivery pipe (4), the waste fluid recovery pipe (5) and the liquid pump (6) are all arranged in the culture chamber.

9. The cell culture device according to claim 1, characterized in that: It also comprises a microscope (10), wherein the microscope (10) comprises a stage, and the culture dish (1) is arranged on the stage.

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