Cell culture dish
By designing an annular groove, liquid separation groove and sliding rod sealing plate structure in the cell culture dish, the problem of poor sealing of existing cell culture dishes is solved, and it is possible to easily add and discharge culture liquid while maintaining sealing, avoid sample contamination and ensure detection effect.
Patent Information
- Application Number
- CN202421433081.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-21
- Publication Date
- 2025-04-15
- Estimated Expiration
- 2034-06-21
AI Technical Summary
Existing cell culture dishes have poor sealing properties and are easily contaminated by harmful microorganisms and impurities in the air, which affects the detection effect.
A cell culture dish was designed, adopting an annular groove and a liquid separation groove structure, combined with the design of a sliding rod and a sealing plate, so that the culture liquid can be easily added and discharged while keeping the interior of the culture dish sealed.
It effectively avoids sample contamination, maintains the sealing inside the petri dish, and ensures the accuracy and reliability of subsequent testing.
Smart Images
Figure CN222758249U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of culture dishes, in particular to a cell culture dish. Background Art
[0002] Cervical cytology is the main means of detecting cervical cancer. Cervical cytology is a detection method that uses smear examination of vaginal exfoliated cells. During the test, mature cells need to be centrifuged to separate the cell nucleus. The mutation of the cell nucleus is used to determine whether the patient has cervical cancer. Medically, the cells scraped from the tester's cervix must first be cultured in a cell culture dish, and then tested after the cells are fully mature.
[0003] A petri dish is a laboratory vessel used for microbial or cell culture. It consists of a flat disc-shaped bottom and a lid, and is generally made of glass or plastic. Existing cell culture dishes have poor sealing properties, which often allow harmful microorganisms to enter the dish. When replenishing the culture medium, the lid of the dish needs to be opened and the culture medium needs to be poured into the dish. Since the dish remains open, impurities in the air can easily fall into the dish, causing the sample to be contaminated, affecting the subsequent detection results.
[0004] Therefore, there is a need for a cell culture dish that can solve the problem of adding supplementary culture fluid while keeping the interior of the dish sealed, so as to avoid contamination of the sample and affect the subsequent detection results. Utility Model Content
[0005] In order to solve the above problems, the utility model proposes a cell culture dish, which can solve the problem of adding supplementary culture fluid while keeping the interior of the culture dish sealed, thereby preventing the sample from being contaminated and affecting the subsequent detection effect.
[0006] In order to achieve the above purpose, the utility model proposes the following specific solutions:
[0007] A cell culture dish, comprising a culture dish, the culture dish comprising a dish wall, an annular groove is provided on the inner side of the middle of the dish wall, a liquid separation groove is provided on the inner wall of one side of the annular groove, the liquid separation groove is connected with the inner side of the culture dish, a fixed block is fixedly provided on the outer side of the dish wall, a liquid injection hole is provided in the middle of the top surface of the fixed block, a sliding groove is connected through the lower end of the liquid injection hole, a sliding hole is provided in the middle of the inner wall of one side of the sliding groove, a sliding rod is movably inserted in the inner side of the sliding hole, a sealing plate is fixedly provided at one end of the sliding rod, the sealing plate is located inside the sliding groove, a liquid inlet hole is provided on the edge of the top surface of the sealing plate, a liquid guide hole is provided in the middle of the bottom surface of the sliding groove, and the liquid guide hole is located at the inner side of the sliding groove. Below the sealing plate, a liquid guide groove is connected through the lower end of the liquid guide hole, one end of the liquid guide groove is connected with the inner side of the annular groove, a guide inclined plate is fixedly provided on the bottom surface of the liquid guide groove, a rectangular tube is fixedly inserted on the inner side of the lower end of the dish wall, the rectangular tube is connected with the inner side of the culture dish, a connecting hole is provided in the middle of one side wall of the rectangular tube, a moving rod is movably inserted on the inner side of the connecting hole, a sealing plug is fixedly provided on one end of the moving rod, the sealing plug is movably sleeved on the inner side of the rectangular tube, a drainage pipe is fixedly provided on the edge of the bottom surface of the rectangular tube, the drainage pipe is connected with the inner side of the rectangular tube, a sealing ring is fixedly provided on the top of the dish wall, and a dish cover is movably sleeved on the outer side of the sealing ring.
[0008] As a preferred technical solution for a cell culture dish of the utility model, a first spring is movably sleeved on the outer side of the sliding rod, one end of the first spring is fixedly connected to the side wall of the fixed block, and the other end of the first spring is fixedly connected to the sliding rod.
[0009] As a preferred technical solution for a cell culture dish of the utility model, a second spring is provided on the outer movable sleeve of the movable rod, the second spring is located on the inner side of the rectangular tube, one end of the second spring is fixedly connected to the inner wall of the rectangular tube, and the other end of the second spring is fixedly connected to the sealing plug.
[0010] As a preferred technical solution for a cell culture dish of the utility model, a support plate is fixedly provided on the outer side of the upper end of the dish wall, and a rotation hole is provided in the middle of one side wall of the support plate.
[0011] As a preferred technical solution for a cell culture dish of the utility model, a buckle is movably inserted into the inner side of the rotating hole, the upper end of the buckle is engaged with the edge of the top surface of the dish cover, and a rotating shaft is fixedly provided on the inner wall of the lower end of the buckle, and the rotating shaft is movably inserted into the inner side of the rotating hole.
[0012] As a preferred technical solution of a cell culture dish of the utility model, a base is movably sleeved on the outer side of the lower end of the culture dish, and a suction cup is fixedly provided in the middle of the bottom surface of the base.
[0013] As a preferred technical solution of a cell culture dish of the utility model, the size of the injection hole is matched with the size of the liquid inlet hole, and the size of the liquid inlet hole is matched with the size of the liquid guide hole.
[0014] As a preferred technical solution of a cell culture dish of the utility model, the liquid separation grooves are provided in plurality, the dish cover is made of transparent material, and the sealing ring, sealing plate and sealing plug are all made of flexible material.
[0015] Compared with the existing technology, the utility model has the following beneficial effects:
[0016] The utility model provides an annular groove on the inner side of the dish wall of the culture dish, and a plurality of evenly distributed liquid separation grooves are provided on the side wall of the annular groove, so that the nutrient solution can evenly flow into the interior of the culture dish. When the nutrient solution needs to be added to the culture dish, the sliding rod is pushed by hand to drive the sealing plate to move. When the sealing plate collides with the inner wall of the sliding groove, the liquid inlet hole on the sealing plate is just located below the liquid injection hole, and the nutrient solution is injected into the liquid injection hole. The nutrient solution enters the liquid guide groove through the liquid injection hole, the liquid inlet hole and the liquid guide hole, and after the flow is guided by the guide inclined plate, the nutrient solution enters the inner side of the annular groove, flows out through the liquid separation groove, and slowly flows to the bottom of the culture dish along the inner side of the dish wall. After the filling is completed, only the hand pushing the sliding rod needs to be removed, and the sealing plate moves under the elastic force of the first spring to re-block the liquid injection hole. The scheme utilizes the blocking effect of the sealing plate to ensure the sealing of the interior of the culture dish without affecting the filling of nutrient solution, thereby avoiding contamination of the sample and affecting the subsequent detection effect.
[0017] The utility model can discharge the waste liquid inside the culture dish through the rectangular tube and the drainage pipe arranged at the lower end of the dish wall; by pulling the moving rod, the sealing plug is driven to move to one side of the drainage pipe in the rectangular tube, and the waste liquid can flow into the drainage pipe through the inner side of the rectangular tube and be discharged outside the culture dish; after the drainage is completed, under the elastic force of the second spring, the sealing plug moves to block one end of the rectangular tube, thereby ensuring the sealing of the inside of the culture dish and preventing the sample from being contaminated.
[0018] The utility model provides a sealing ring at the top of the dish wall. When the dish cover is covered on the culture dish, the sealing ring is tightly fitted with the inner wall of the dish cover to ensure the sealing performance. In addition, the buckle is clamped on the dish cover to prevent the dish cover from being accidentally opened, thereby avoiding the occurrence of accidents. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] In order to better describe the technical solution of the utility model in detail, the present invention is further explained below in conjunction with the accompanying drawings and embodiments.
[0020] Figure 1 It is a three-dimensional diagram of the overall structure provided by the utility model;
[0021] Figure 2 It is a three-dimensional cross-sectional view of the utility model;
[0022] Figure 3 A three-dimensional diagram of the base of the utility model;
[0023] Figure 4 For this utility model Figure 2 The enlarged schematic diagram of point A in the middle;
[0024] Figure 5 For this utility model Figure 2 The enlarged schematic diagram of point B in the middle;
[0025] Figure 6 For this utility model Figure 2 Enlarged schematic diagram of point C in the middle.
[0026] In the figure: 1. culture dish; 11. fixed block; 111. injection hole; 112. sliding groove; 113. sliding hole; 114. liquid guide hole; 115. liquid guide groove; 116. guide inclined plate; 12. sliding rod; 121. first spring; 122. sealing plate; 123. liquid inlet hole; 13. rectangular cylinder; 131. connecting hole; 132. drain pipe; 14. moving rod; 141. sealing plug; 142. second spring; 15. support plate; 151. rotating hole; 16. buckle; 161. rotating shaft; 17. annular groove; 171. liquid separation groove; 18. sealing ring; 19. dish wall; 2. base; 21. suction cup; 3. dish cover. DETAILED DESCRIPTION
[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0028] Example
[0029] See also Figure 1-6 As shown, the utility model provides the following technical solutions: a cell culture dish, comprising a culture dish 1, a base 2 is movably sleeved on the outer side of the lower end of the culture dish 1, and a suction cup 21 is fixedly provided in the middle of the bottom surface of the base 2. The suction cup 21 is used to fix the base 2 in this solution, so that the culture dish 1 can remain stable on the base 2 during cell culture operations, and will not shake or displace during operation, thereby affecting the progress of cell culture.
[0030] Reference Figure 1 , Figure 2 and Figure 4As shown, specifically, the culture dish 1 includes a dish wall 19, and an annular groove 17 is provided on the inner side of the middle of the dish wall 19 for dispersing and guiding the nutrient solution. A liquid separation groove 171 is provided on the inner wall of one side of the annular groove 17. There are multiple liquid separation grooves 171, which are conducive to evenly distributing the nutrient solution to the inner side of the culture dish 1. The liquid separation grooves 171 are connected to the inner side of the culture dish 1. A fixed block 11 is fixed on the outer side of the dish wall 19 for supporting and connecting the sealing plate 122. A liquid injection hole 111 is provided in the middle of the top surface of the fixed block 11 for adding nutrient solution. The lower end of the injection hole 111 is connected through a sliding groove 112 to facilitate the movement of the sealing plate 122. A sliding hole 113 is provided in the middle of the inner wall of one side of the sliding groove 112 to facilitate the movement of the sliding rod 12. The sliding hole 113 is movably inserted into the inner side of the sliding rod 12 to drive the sealing plate 122 to move. The outer side of the sliding rod 12 is movably sleeved with a first spring 121 to provide an elastic driving force for the sliding rod 12. One end of the first spring 121 is fixedly connected to the side wall of the fixed block 11, and the other end of the first spring 121 is fixedly connected to the side wall of the fixed block 11. The end is fixedly connected to the sliding rod 12, and a sealing plate 122 is fixedly provided at one end of the sliding rod 12, which is used to block the injection hole 111 and seal the culture dish 1. The sealing plate 122 is located inside the sliding groove 112, and a liquid inlet hole 123 is provided on the edge of the top surface of the sealing plate 122 to facilitate the passage of the nutrient solution. The size of the injection hole 111 is adapted to the size of the liquid inlet hole 123. A liquid guide hole 114 is provided in the middle of the bottom surface of the sliding groove 112 to guide the nutrient solution to the inside of the liquid guide groove 115. The liquid guide hole 114 is located below the sealing plate 122, and the liquid inlet hole 123 is provided on the top edge of the sealing plate 122 to facilitate the passage of the nutrient solution. The size of the liquid hole 123 is adapted to the size of the liquid guide hole 114. The lower end of the liquid guide hole 114 is connected through a liquid guide groove 115 for guiding the nutrient solution to the inner side of the annular groove 17. One end of the liquid guide groove 115 is connected to the inner side of the annular groove 17. A guide inclined plate 116 is fixedly provided on the bottom surface of the liquid guide groove 115 to ensure that the nutrient solution inside the liquid guide groove 115 flows to the inner side of the annular groove 17. This solution ensures the sealing of the interior of the culture dish 1 without affecting the filling of the nutrient solution, thereby avoiding contamination of the sample and affecting the subsequent detection effect.
[0031] Reference Figure 1 , Figure 2 and Figure 5As shown, specifically, a rectangular tube 13 is fixedly inserted into the inner side of the lower end of the dish wall 19, which is used for the movement of the sealing plug 141 and the outflow of waste liquid. The rectangular tube 13 is connected with the inner side of the culture dish 1. A connecting hole 131 is provided in the middle of one side wall of the rectangular tube 13 to facilitate the movement of the moving rod 14. A moving rod 14 is movably inserted into the inner side of the connecting hole 131 to drive the sealing plug 141 to move. A sealing plug 141 is fixedly provided at one end of the moving rod 14 to block the inner side of the rectangular tube 13 to prevent the leakage of the nutrient solution and ensure the sealing of the culture dish 1. The sealing plug 141 is movably sleeved on the inner side of the rectangular tube 13, and the sealing ring 18 and the sealing plate 122 are provided. The movable rod 14 and the sealing plug 141 are both made of flexible materials. A second spring 142 is movably sleeved on the outer side of the movable rod 14 for providing an elastic driving force for the sealing plug 141. The second spring 142 is located on the inner side of the rectangular tube 13. One end of the second spring 142 is fixedly connected to the inner wall of the rectangular tube 13. The other end of the second spring 142 is fixedly connected to the sealing plug 141. A drain pipe 132 is fixedly provided on the edge of the bottom surface of the rectangular tube 13 for discharging waste liquid. The drain pipe 132 is connected to the inner side of the rectangular tube 13. This solution can ensure the sealing of the inside of the culture dish 1 and prevent the sample from being contaminated, while facilitating the discharge of waste liquid inside the culture dish 1.
[0032] Reference Figure 1 , Figure 2 and Figure 6 As shown, specifically, a sealing ring 18 is fixedly provided at the top of the dish wall 19 for sealing the gap between the dish cover 3 and the dish wall 19, and a dish cover 3 is movably sleeved on the outer side of the sealing ring 18. The dish cover 3 is made of a transparent material and is used to prevent dust from falling into the inner side of the culture dish 1 and facilitate observation of the inner side of the culture dish 1. A support plate 15 is fixedly provided on the outer side of the upper end of the dish wall 19 for supporting the buckle 16. A rotating hole 151 is provided in the middle of one side wall of the support plate 15 for facilitating the rotation of the rotating shaft 161. A buckle 16 is movably inserted into the inner side of the rotating hole 151, and the upper end of the buckle 16 is engaged with the edge of the top surface of the dish cover 3 to fix the dish cover 3. A rotating shaft 161 is fixedly provided on the inner wall of the lower end of the buckle 16, and the rotating shaft 161 is movably inserted into the inner side of the rotating hole 151 to drive the buckle 16 to rotate. The sealing ring 18 of this scheme fits tightly with the inner wall of the dish cover 3 to ensure the sealing performance. In addition, the buckle 16 is stuck on the dish cover 3 to prevent the dish cover 3 from being accidentally opened, thereby avoiding the occurrence of unexpected situations.
[0033] The working principle and use process of this utility model:
[0034] When in use, the base 2 is fixed on the workbench by the suction cup 21 to prevent the culture dish 1 from being displaced during operation, and then the culture dish 1 is placed on the base 2, the sample is placed on the bottom of the culture dish 1, and then the dish cover 3 is covered, and the two buckles 16 are snapped on the dish cover 3 to complete the sealing of the culture dish 1. When nutrient solution needs to be added, first pull the moving rod 14 to drive the sealing plug 141 to move to the side of the drainage pipe 132 inside the rectangular tube 13, and the waste liquid can flow into the drainage pipe 132 through the inside of the rectangular tube 13 and be discharged from the outside of the culture dish 1. After the drainage is completed, under the elastic force of the second spring 142, the sealing plug 141 moves to block one end of the rectangular tube 13 for sealing, and then the slide is pushed by hand. The movable rod 12 drives the sealing plate 122 to move. When the sealing plate 122 collides with the inner wall of the sliding groove 112, the liquid inlet hole 123 on the sealing plate 122 is just located below the injection hole 111, and the nutrient solution is injected into the injection hole 111. The nutrient solution enters the liquid guide groove 115 through the injection hole 111, the liquid inlet hole 123 and the liquid guide hole 114, and is guided by the guide inclined plate 116. The nutrient solution enters the inner side of the annular groove 17, flows out through the liquid separation groove 171, and slowly flows to the bottom of the culture dish 1 along the inner side of the dish wall 19. After the filling is completed, just move the hand that pushes the sliding rod 12, and the sealing plate 122 moves under the elastic force of the first spring 121 to re-block the injection hole 111 for sealing.
[0035] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.
Claims
1. A cell culture dish, comprising a culture dish (1), characterized in that: The culture dish (1) comprises a dish wall (19), an annular groove (17) is provided on the inner side of the middle part of the dish wall (19), a liquid separation groove (171) is provided on the inner wall of one side of the annular groove (17), the liquid separation groove (171) is connected with the inner side of the culture dish (1), a fixed block (11) is fixedly provided on the outer side of the dish wall (19), a liquid injection hole (111) is provided in the middle part of the top surface of the fixed block (11), a sliding groove (112) is provided at the lower end of the liquid injection hole (111), the sliding groove (112) is connected with the inner side of the liquid injection hole (111), and the sliding groove (112) is provided on the inner side of the liquid injection hole (111). A sliding hole (113) is provided in the middle of the inner wall of one side of the movable groove (112), a sliding rod (12) is movably inserted in the inner side of the sliding hole (113), a sealing plate (122) is fixedly provided at one end of the sliding rod (12), the sealing plate (122) is located inside the sliding groove (112), a liquid inlet hole (123) is provided at the edge of the top surface of the sealing plate (122), a liquid guide hole (114) is provided in the middle of the bottom surface of the sliding groove (112), and the liquid guide hole (114) is located on the sealing plate ( 122), a liquid guide groove (115) is connected and connected at the lower end of the liquid guide hole (114), one end of the liquid guide groove (115) is connected to the inner side of the annular groove (17), a guide inclined plate (116) is fixedly provided on the bottom surface of the liquid guide groove (115), a rectangular tube (13) is fixedly inserted and connected to the inner side of the lower end of the dish wall (19), the rectangular tube (13) is connected to the inner side of the culture dish (1), a connecting hole (131) is provided in the middle of one side wall of the rectangular tube (13), and the connecting hole (131) is provided in the middle of the one side wall of the rectangular tube (13). A moving rod (14) is movably inserted into the inner side of the rectangular tube (131), a sealing plug (141) is fixedly provided at one end of the moving rod (14), the sealing plug (141) is movably sleeved on the inner side of the rectangular tube (13), a drainage pipe (132) is fixedly provided at the bottom edge of the rectangular tube (13), the drainage pipe (132) is connected to the inner side of the rectangular tube (13), a sealing ring (18) is fixedly provided at the top end of the dish wall (19), and a dish cover (3) is movably sleeved on the outer side of the sealing ring (18).
2. A cell culture dish according to claim 1, characterized in that: A first spring (121) is movably sleeved on the outer side of the sliding rod (12); one end of the first spring (121) is fixedly connected to the side wall of the fixed block (11); and the other end of the first spring (121) is fixedly connected to the sliding rod (12).
3. A cell culture dish according to claim 2, characterized in that: A second spring (142) is movably sleeved on the outer side of the moving rod (14), and the second spring (142) is located on the inner side of the rectangular tube (13). One end of the second spring (142) is fixedly connected to the inner wall of the rectangular tube (13), and the other end of the second spring (142) is fixedly connected to the sealing plug (141).
4. A cell culture dish according to claim 3, characterized in that: A support plate (15) is fixedly provided on the outer side of the upper end of the dish wall (19), and a rotation hole (151) is provided in the middle of one side wall of the support plate (15).
5. A cell culture dish according to claim 4, characterized in that: A buckle (16) is movably inserted inside the rotating hole (151), the upper end of the buckle (16) is engaged with the edge of the top surface of the dish cover (3), and a rotating shaft (161) is fixedly provided on the inner wall of the lower end of the buckle (16), and the rotating shaft (161) is movably inserted inside the rotating hole (151).
6. A cell culture dish according to claim 5, characterized in that: A base (2) is movably sleeved on the outer side of the lower end of the culture dish (1), and a suction cup (21) is fixedly provided on the middle part of the bottom surface of the base (2).
7. A cell culture dish according to claim 6, characterized in that: The size of the liquid injection hole (111) matches the size of the liquid inlet hole (123), and the size of the liquid inlet hole (123) matches the size of the liquid guide hole (114).
8. A cell culture dish according to claim 7, characterized in that: The liquid separation grooves (171) are provided in plurality, the dish cover (3) is made of a transparent material, and the sealing ring (18), the sealing plate (122) and the sealing plug (141) are all made of flexible materials.