Cell metabolism detection equipment based on bioluminescence principle

Abstract

The utility model discloses cell metabolism detection equipment based on a bioluminescence principle, which comprises a luminous microplate reader main body, the bottom of the luminous microplate reader main body is provided with a balance component, the bottom of the luminous microplate reader main body is fixedly connected with a top seat at equal intervals, the bottom of the top seat is fixedly connected with a screw rod, and the screw rod is fixedly connected with a screw rod. The outer surface of the screw rod is in threaded connection with a threaded cylinder, and a first bearing is fixedly mounted on the outer surface of the threaded cylinder. According to the light-emitting microplate reader disclosed by the utility model, by arranging the balance assembly, when the light-emitting microplate reader main body is used, if the light-emitting microplate reader main body inclines, an alarm at a corresponding lower position can give an alarm, so that a worker can conveniently rotate a corresponding turntable to adjust the horizontal position of the light-emitting microplate reader main body; and the automatic horizontal structure can ensure that the luminous microplate reader main body is stably supported in a horizontal state, so that the shaking or inclination of the reader caused by unbalanced bottom support is avoided, and the stability and accuracy of an experiment are ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of biology, in particular to a cell metabolism detection device based on the bioluminescence principle. Background Art

[0002] Cell metabolism detection equipment based on the principle of bioluminescence is usually a device that uses the bioluminescent signal generated by a specific enzyme system inside the cell to detect cell metabolism. These devices are often used in research on cell viability, enzyme activity, cell stress response, etc. Among them, the luminescent microplate reader is a common instrument for measuring the intensity of bioluminescent signals. It is based on the principle of bioluminescence and detects the bioluminescent signal generated by a specific enzyme system in the biological sample to evaluate the metabolic activity, enzyme activity and other biological processes in the sample. The luminescent microplate reader is usually widely used in biomedical research, drug screening, gene expression analysis and other fields. When using the luminescent microplate reader, the bioluminescent reaction in the sample will produce a light signal. The luminescent microplate reader will measure and record the intensity of this light signal, and infer the biological activity in the sample by analyzing the intensity of this signal.

[0003] After searching, the patent number is CN212111437U, and the specific name is a kind of microplate reader, including a microplate reader body, a base is provided under the microplate reader body, and a placement groove for placing the microplate reader body is provided on the upper surface of the base, and the placement groove is plugged with the bottom of the microplate reader body; a sliding groove is provided on the back of the base, and the sliding groove is located below the placement groove and is not connected with the placement groove. A dustproof component is slidably matched in the sliding groove, and the dustproof component is used to prevent dust from the microplate reader body, and two connecting grooves are provided on the upper surface of the base. The length direction of the connecting groove is along the sliding direction of the sliding plate, and the connecting groove is connected with the sliding groove; a fixing component for fixing the dustproof component is provided in the connecting groove;

[0004] Traditional ELISA instruments are placed directly on the work surface for testing. If the equipment is tilted, the staff cannot detect and adjust it in time, which may cause the reaction materials to be unevenly deposited at the bottom of the container, affecting the uniformity of the reaction and the accuracy of the final test results. A tilted ELISA instrument may cause the liquid in the test tube to overflow and uneven reaction, ultimately leading to failure of the experiment or inaccurate data. Utility Model Content

[0005] In view of the shortcomings of the existing technology, the utility model provides a cell metabolism detection device based on the principle of bioluminescence, which solves the problem that traditional ELISA instruments are placed directly on the workbench for detection. If the equipment is tilted, the staff cannot find and adjust it in time, which ultimately leads to the failure of the experiment or inaccurate data.

[0006] To achieve the above objectives, the utility model is implemented through the following technical solutions: a cell metabolism detection device based on the principle of bioluminescence, comprising a luminescent microplate reader body, a balancing assembly is installed at the bottom of the luminescent microplate reader body, a top seat is equidistantly fixedly connected to the bottom of the luminescent microplate reader body, a screw is fixedly connected to the bottom of the top seat, a threaded barrel is threadedly connected to the outer surface of the screw, a first bearing is fixedly installed to the outer surface of the threaded barrel, a first bevel gear is fixedly connected to the outer surface of the threaded barrel, a base is fixedly installed to the outer surface of the first bearing, an alarm is fixedly installed to the outer surface of the base, and a bottom plate is fixedly connected to the bottom of the base, a second bearing is fixedly installed inside the base, a limiting cylinder is fixedly connected inside the limiting cylinder, a sliding rod is slidably connected inside the limiting cylinder, one end of the sliding rod is fixedly connected to a rotating rod, the other end of the sliding rod is fixedly connected to a rotating disk, and the outer surface of the rotating rod is fixedly connected to a second bevel gear;

[0007] The balance component includes a circular plate fixedly connected to the bottom of the luminescent microplate reader body, a rolling groove is provided inside the circular plate, a metal ball is placed inside the rolling groove, grooves are equidistantly provided on the inner wall of the rolling groove, and a touch switch is fixedly installed inside the groove.

[0008] Preferably, the outer surface of the rotating rod is fixedly connected to a third bevel gear, the interior of the base is fixedly installed with a third bearing, the interior of the third bearing is fixedly connected to a threaded rod, the top of the threaded rod is fixedly connected to a fourth bevel gear, the interior of the base is slidably connected to a square threaded tube, and the bottom of the square threaded tube is rotatably connected to a roller.

[0009] Preferably, the surface of the second bevel gear is meshedly connected to the surface of the first bevel gear, and one end of the screw rod away from the top seat is movably connected to the inside of the base.

[0010] Preferably, a battery is fixedly installed inside the circular plate, and the touch switch is electrically connected to the alarm through the battery.

[0011] Preferably, the number of the grooves is equal to that of the alarms, and the metal balls are iron balls.

[0012] Preferably, the sliding rod is square, the inner wall of the limiting cylinder matches the shape of the sliding rod, and the rotating rod, the second bevel gear and the third bevel gear are all movably connected inside the base. Beneficial Effects

[0013] The utility model provides a cell metabolism detection device based on the principle of bioluminescence. Compared with the prior art, it has the following beneficial effects:

[0014] 1. This cell metabolism detection equipment based on the principle of bioluminescence is equipped with a balance component. When the main body of the luminescent microplate reader is in use, if there is a tilt, the corresponding low alarm will sound an alarm, which is convenient for the staff to turn the corresponding turntable to adjust the horizontal position of the main body of the luminescent microplate reader. The automatic horizontal structure can ensure that the main body of the luminescent microplate reader is stably supported in a horizontal state, avoiding the shaking or tilting of the instrument due to the imbalance of the bottom support, and ensuring the stability and accuracy of the experiment.

[0015] 2. The cell metabolism detection device based on the principle of bioluminescence, by setting a square threaded tube and a circular plate, etc., can drive the third bevel gear and the fourth bevel gear to engage by pulling the turntable, and can drive the threaded rod to slide. When the bottom of the roller contacts the ground, it is convenient to move and position the main body of the luminescent microplate reader, and it is also convenient to clean the bottom of the main body of the luminescent microplate reader. Keeping the bottom of the equipment clean can effectively prevent dust, debris, etc. from affecting the normal operation of the equipment, thereby extending the service life of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the utility model;

[0017] Figure 2 It is a structural schematic diagram of the balancing component in the utility model;

[0018] Figure 3 It is a schematic diagram of the connection between the top seat and the base in the utility model;

[0019] Figure 4 It is a cross-sectional view of the base in the utility model;

[0020] Figure 5 for Figure 4 Enlarged view of point A in the middle.

[0021] In the figure: 1. luminous microplate reader body; 2. balance assembly; 21. round plate; 22. rolling groove; 23. metal ball; 24. groove; 25. touch switch; 3. top seat; 4. screw; 5. threaded cylinder; 6. first bearing; 7. base; 71. alarm; 8. first bevel gear; 9. bottom plate; 10. second bearing; 11. limit cylinder; 12. sliding rod; 13. rotating rod; 14. turntable; 15. second bevel gear; 16. third bevel gear; 17. fourth bevel gear; 18. threaded rod; 19. third bearing; 20. square threaded tube; 201. roller. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] See also Figure 1-5 The utility model provides a technical solution: a cell metabolism detection device based on the principle of bioluminescence, comprising a luminescent microplate reader body 1, a balancing component 2 is installed at the bottom of the luminescent microplate reader body 1, a top seat 3 is equidistantly fixedly connected to the bottom of the luminescent microplate reader body 1, a screw 4 is fixedly connected to the bottom of the top seat 3, a threaded cylinder 5 is threadedly connected to the outer surface of the screw 4, a first bearing 6 is fixedly installed on the outer surface of the threaded cylinder 5, a first bevel gear 8 is fixedly connected to the outer surface of the threaded cylinder 5, a base 7 is fixedly connected to the outer surface of the first bearing 6, an end of the screw 4 away from the top seat 3 is movably connected to the inside of the base 7, an alarm 71 is fixedly installed on the outer surface of the base 7, a bottom plate 9 is fixedly connected to the bottom of the base 7, a second bearing 10 is fixedly installed inside the base 7, a limiting cylinder 11 is fixedly connected inside the limiting cylinder 11, a sliding rod 12 is slidably connected inside the limiting cylinder 11, the sliding rod 12 is square, and the inner wall of the limiting cylinder 11 matches the shape of the sliding rod 12, one end of the sliding rod 12 is fixedly connected to a rotating rod 13, and the other end of the sliding rod 12 is fixedly connected to a turntable 1 4. The outer surface of the rotating rod 13 is fixedly connected with the second bevel gear 15, and the surface of the second bevel gear 15 is meshedly connected with the surface of the first bevel gear 8. The balancing component 2 includes a circular plate 21 fixedly connected to the bottom of the luminescent microplate reader body 1, and a rolling groove 22 is provided inside the circular plate 21. A metal ball 23 is placed inside the rolling groove 22. The metal ball 23 is an iron ball. The inner wall of the rolling groove 22 is equidistantly provided with grooves 24, and a touch switch 25 is fixedly installed inside the groove 24. A battery is fixedly installed inside the circular plate 21. The touch switch 25 is electrically connected to the alarm 71 through the battery. The number of the grooves 24 and the alarm 71 is equal. By setting the balancing component 2, when the luminescent microplate reader body 1 is in use, if there is a tilt, the alarm 71 at the corresponding lower position will sound an alarm, so that the staff can turn the corresponding turntable 14 to adjust the horizontal position of the luminescent microplate reader body 1. The automatic horizontal structure can ensure that the luminescent microplate reader body 1 is stably supported in a horizontal state, avoid shaking or tilting of the instrument due to unbalanced bottom support, and ensure the stability and accuracy of the experiment.

[0024] The outer surface of the rotating rod 13 is fixedly connected with the third bevel gear 16, and the rotating rod 13, the second bevel gear 15 and the third bevel gear 16 are all movably connected to the inside of the base 7. The inside of the base 7 is fixedly installed with a third bearing 19, and the inside of the third bearing 19 is fixedly connected with a threaded rod 18, and the top of the threaded rod 18 is fixedly connected with a fourth bevel gear 17. The inside of the base 7 is slidably connected with a square threaded tube 20, and the bottom of the square threaded tube 20 is rotatably connected with a roller 201. By arranging the square threaded tube 20 and the circular plate 21, etc., by pulling the turntable 14, the third bevel gear 16 can be driven to engage with the fourth bevel gear 17, and the threaded rod 18 can be driven to slide. When the bottom of the roller 201 contacts the ground, it is convenient to move and position the luminescent microplate reader main body 1, and it is also convenient to clean the bottom of the luminescent microplate reader main body 1. Keeping the bottom of the equipment clean can effectively prevent dust, debris, etc. from affecting the normal operation of the equipment, thereby extending the service life of the equipment.

[0025] Before using the luminescent microplate reader body 1, first adjust the horizontal position of the luminescent microplate reader body 1. When adjusting, place the luminescent microplate reader body 1 in the specified position. If the luminescent microplate reader body 1 is not in a horizontal position at this time, the metal ball 23 will slide in the direction of the corresponding lower position and slide into the groove 24 of the angle. At the same time, the touch switch 25 is started, and the alarm 71 at the corresponding position is in an alarm state, so that the staff can find the position easily. Then the staff rotates the turntable 14 at that position. The turntable 14 drives the first bevel gear 8 to rotate through the sliding rod 12, the rotating rod 13 and the second bevel gear 15. The first bevel gear 8 drives the threaded barrel 5 to rotate, so that the screw rod 4 drives the top seat 3 to slide upward, and the angle of the luminescent microplate reader body 1 is lifted. When it is lifted to the horizontal position, the metal ball 23 is in a horizontal position inside the rolling groove 22 and is not in contact with the touch switch 25. The alarm 71 is also in a closed state. At this time, the luminescent microplate reader body 1 can be used for detection. When the luminescent microplate reader body 1 needs to be moved, the sliding rod 12 and the rotating rod 13 are driven by the turntable 14 to slide in a direction away from the first bevel gear 8, so that the third bevel gear 16 is engaged with the fourth bevel gear 17. The base 7 can be driven to rotate by rotating the turntable 14. The base 7 will drive the square threaded tube 20 and the roller 201 to slide downward. When the bottom of the roller 201 contacts the ground, it is convenient to move and position the luminescent microplate reader body 1, and it is also convenient to clean the bottom of the luminescent microplate reader body 1. Keeping the bottom of the equipment clean can effectively avoid dust, debris, etc. that affect the normal operation of the equipment, thereby extending the service life of the equipment.

[0026] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

Claims

1. A cell metabolism detection device based on the bioluminescence principle, comprising a luminescent microplate reader body (1), characterized in that: A balancing assembly (2) is installed at the bottom of the luminescent microplate reader body (1); a top seat (3) is fixedly connected to the bottom of the luminescent microplate reader body (1) at equal intervals; a screw (4) is fixedly connected to the bottom of the top seat (3); a threaded barrel (5) is threadedly connected to the outer surface of the screw (4); a first bearing (6) is fixedly installed on the outer surface of the threaded barrel (5); a first bevel gear (8) is fixedly connected to the outer surface of the threaded barrel (5); a base (7) is fixedly connected to the outer surface of the first bearing (6); and the outer surface of the base (7) is The bottom of the base (7) is fixedly connected to a bottom plate (9) with an alarm (71) fixedly mounted on the surface, a second bearing (10) is fixedly mounted inside the base (7), a limiting cylinder (11) is fixedly connected inside the second bearing (10), a sliding rod (12) is slidably connected inside the limiting cylinder (11), one end of the sliding rod (12) is fixedly connected to a rotating rod (13), the other end of the sliding rod (12) is fixedly connected to a rotating disk (14), and the outer surface of the rotating rod (13) is fixedly connected to a second bevel gear (15); The balancing component (2) comprises a circular plate (21) fixedly connected to the bottom of the luminescent microplate reader body (1); a rolling groove (22) is provided inside the circular plate (21); a metal ball (23) is placed inside the rolling groove (22); grooves (24) are provided at equal intervals on the inner wall of the rolling groove (22); a touch switch (25) is fixedly installed inside the groove (24).

2. The cell metabolism detection device based on the bioluminescence principle according to claim 1, characterized in that: The outer surface of the rotating rod (13) is fixedly connected to a third bevel gear (16); a third bearing (19) is fixedly installed inside the base (7); a threaded rod (18) is fixedly connected inside the third bearing (19); a fourth bevel gear (17) is fixedly connected to the top of the threaded rod (18); a square threaded tube (20) is slidably connected inside the base (7); and a roller (201) is rotatably connected to the bottom of the square threaded tube (20).

3. The cell metabolism detection device based on the bioluminescence principle according to claim 1, characterized in that: The surface of the second bevel gear (15) is meshingly connected to the surface of the first bevel gear (8), and the end of the screw rod (4) away from the top seat (3) is movably connected to the inside of the base (7).

4. The cell metabolism detection device based on the bioluminescence principle according to claim 1, characterized in that: A battery is fixedly mounted inside the circular plate (21), and the touch switch (25) is electrically connected to the alarm (71) via the battery.

5. The cell metabolism detection device based on the bioluminescence principle according to claim 1, characterized in that: The number of the grooves (24) is equal to the number of the alarms (71), and the metal balls (23) are iron balls.

6. The cell metabolism detection device based on the bioluminescence principle according to claim 2, characterized in that: The sliding rod (12) is square, the inner wall of the limiting cylinder (11) matches the shape of the sliding rod (12), and the rotating rod (13), the second bevel gear (15) and the third bevel gear (16) are all movably connected inside the base (7).

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