Strain culture container and constant-temperature incubator

Abstract

The utility model provides a strain culture container and a constant-temperature incubator. The strain culture container comprises a barrel, an end cover, a gas uniformizing core pipe and breathable cotton. The cylinder body adopts a structure with a hollow interior and an opening at one end, and the interior of the cylinder body is used for containing strains; the end cover is detachably connected to the open end of the barrel, a through hole communicated with the interior of the barrel is formed in the end cover, and a connector is inserted into the through hole; the joint is opposite to the cylinder body and extends out of the through hole; the gas uniformizing core pipe is arranged in the cylinder, the axial direction of the gas uniformizing core pipe is parallel to the opening direction of the cylinder, and one end of the gas uniformizing core pipe is rotationally connected with the end cover and communicates with the through hole; a plurality of gas outlet parts are arranged on the peripheral wall of the gas uniformizing core pipe; the breathable cotton is arranged in the through hole and located between the connector and the air uniformizing core pipe. When the connector is connected with the oxygen generator, oxygen generated by the oxygen generator can penetrate through the breathable cotton to be input into the cylinder body through each air outlet part. According to the strain culture container, the oxygen introduction process is smooth, and the concentration of oxygen introduced into the culture container can be more uniform.

Description

Technical Field

[0001] The utility model belongs to the technical field of bacterial culture, and more specifically, relates to a bacterial culture container and a constant temperature culture box. Background Art

[0002] Strain culture technology is a technology that cultivates bacterial strains in a laboratory environment. It utilizes the unique biological evolution mechanism of microorganisms to discover and develop microbial resources, providing important raw materials and experimental basis for research in the fields of strain development, microbial application and microbial biotechnology. The culture of strains generally adopts a culture medium system to obtain a good environment suitable for cultivating microorganisms, and promotes the growth and reproduction of strains by changing the water-soluble substances contained in the culture medium.

[0003] At present, after the culture container is inoculated with aerobic bacteria, the opening of the container needs to be sealed with an end cap with a vent. In order to prevent miscellaneous bacteria from entering the culture container, a cotton plug will be inserted into the vent. Then the experimenter will input oxygen into the culture container through the vent to promote the growth and reproduction of aerobic bacteria.

[0004] The inventors have discovered that the existing culture container has only one vent hole, and the vent hole is located at the opening of the culture container. During the oxygenation process, oxygen will first slowly enter the interior of the container from the opening of the culture container, resulting in the oxygen concentration in the area near the end cover inside the culture container being higher than the oxygen concentration in the area far from the end cover, causing the growth and reproduction speeds of the bacteria inside the culture container to be different, affecting the culture results of the bacteria. Utility Model Content

[0005] The purpose of the present application is to provide a strain culture container and a constant temperature incubator to solve the technical problem in the prior art that uneven oxygenation of the strain culture container leads to inconsistent oxygen concentration inside the culture container.

[0006] In order to achieve the above-mentioned purpose, the technical solution adopted in the present application is: to provide a bacterial culture container, comprising:

[0007] The cylinder body is hollow inside and open at one end, and the inside is used to hold the bacteria;

[0008] An end cap is detachably connected to the open end of the cylinder, and has a through hole communicating with the interior of the cylinder, and a connector is inserted into the through hole; the connector extends to the outside of the through hole away from the cylinder, and the protruding end of the connector is used to connect to the oxygen concentrator;

[0009] an air-uniform core tube, arranged inside the cylinder, with its axial direction parallel to the opening of the cylinder, and one end of which is rotatably connected to the end cover and communicated with the through hole; a plurality of air outlets are provided on the outer peripheral wall of the air-uniform core tube; and

[0010] Breathable cotton, arranged in the through hole, and located between the joint and the air-uniform core tube;

[0011] Wherein, when the joint is connected to the oxygen generator, the oxygen generated by the oxygen generator can pass through the breathable cotton and be input into the interior of the cylinder through each of the air outlets.

[0012] Preferably, the plurality of air outlet portions are arranged at intervals along the axial direction of the air uniforming core tube; each of the air outlet portions is a plurality of air outlet pipes arranged at intervals along the circumferential direction of the air uniforming core tube, and each of the air outlet pipes is connected to the interior of the air uniforming core tube.

[0013] Preferably, the air outlet pipe comprises:

[0014] a straight tube section, which is arranged on the outer wall of the gas-uniform core tube and extends outward in the radial direction of the gas-uniform core tube; and

[0015] The direction-changing section has one end connected to one end of the straight tube section facing away from the gas-uniform core tube, and the other end thereof is oriented perpendicular to the axial direction of the straight tube section.

[0016] Preferably, the end cover has a groove communicating with the through hole on one side facing the cylinder, the gas-uniform core tube is embedded in the groove, and has a ring platform extending radially outward; and the end cover is detachably connected with a ring-shaped fixing plate;

[0017] When the annular platform is embedded in the groove and the end cover is connected to the fixing plate, the fixing plate is arranged around the gas-distributing core tube, and the fixing plate abuts against the annular platform to limit the gas-distributing core tube from moving away from the end cover.

[0018] Preferably, the fixing plate has a slot for allowing the air outlet to pass through.

[0019] Preferably, the groove bottom of the groove is provided with a plurality of thread grooves; the fixing plate is provided with a plurality of alignment holes corresponding to the plurality of thread grooves in one-to-one correspondence, and the fixing plate further comprises a plurality of fastening bolts corresponding to the plurality of alignment holes in one-to-one correspondence;

[0020] When the plurality of alignment holes are connected to the plurality of thread grooves in a one-to-one correspondence, each of the fastening bolts passes through the corresponding alignment hole and is threadedly connected to the corresponding thread groove.

[0021] Preferably, the end of the end cover facing the cylinder has a threaded sleeve surrounding the outer circumference of the cylinder and threadedly connected to the cylinder.

[0022] Preferably, a sealing gasket is further provided between the end cover and the cylinder; when the end cover is connected to the cylinder, two side surfaces of the sealing gasket abut against the end cover and the cylinder respectively to block the gap between the end cover and the cylinder.

[0023] Preferably, the outer wall of the joint has a plurality of annular grooves arranged at intervals along the axial direction of the cylinder.

[0024] In the embodiment of the present application, after the cylinder is inoculated with the bacteria, the end cap is installed at the open end of the cylinder, and the end cap is provided with a connector for connecting to the oxygen generator, and oxygen can be input into the interior of the uniform air core tube through the connector; the uniform air core tube is arranged inside the cylinder and is rotatably connected to the end cap; the outer peripheral wall of the uniform air core tube is provided with multiple air outlets, and oxygen can be evenly discharged to various positions inside the cylinder through the multiple air outlets, so that the oxygen concentration inside the cylinder remains consistent; the breathable cotton between the connector and the end cap can prevent miscellaneous bacteria from entering the interior of the cylinder while also reducing the barrier effect on oxygen, making oxygen flow smoother compared to the cotton plug in the prior art.

[0025] Compared with the prior art, the strain culture container provided in the embodiment of the present application has a smoother oxygenation process when strain culture is performed using the container, and can evenly disperse the oxygen introduced into the culture container.

[0026] The technical solution adopted in the present application also provides a constant temperature incubator, including any of the aforementioned bacterial culture containers.

[0027] The beneficial effects of the constant temperature incubator provided in this embodiment are the same as those of the aforementioned bacterial culture container, and will not be repeated here. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0029] Figure 1 A schematic diagram of the three-dimensional structure of a bacterial culture container provided in an embodiment of the present application;

[0030] Figure 2 A schematic diagram of the main structure of the bacterial culture container provided in the embodiment of the present application;

[0031] Figure 3 For along Figure 2 The cross-sectional structure diagram along the AA line;

[0032] Figure 4A schematic diagram of the explosion structure of the bacterial culture container provided in the embodiment of the present application;

[0033] Figure 5 for Figure 4 Schematic diagram of the enlarged structure of area B in the middle.

[0034] Among them, the reference numerals in the figure are:

[0035] 1. Cylinder; 2. End cover; 21. Through hole; 22. Threaded groove; 23. Threaded sleeve; 24. Groove; 3. Gas core tube; 31. Ring platform; 32. Air outlet pipe; 321. Straight pipe section; 322. Direction change section; 4. Joint; 41. Annular groove; 5. Fixing plate; 51. Notch; 52. Alignment hole; 53. Fastening bolt; 6. Breathable cotton; 7. Sealing gasket; 8. Oxygen generator. DETAILED DESCRIPTION

[0036] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0037] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0038] It should be understood that the orientation or position relationship indicated by terms such as "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0039] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0040] Please also read Figures 1 to 5 The bacterial culture container provided in the present application is now described. The bacterial culture container comprises a cylinder 1, an end cover 2, an air-uniform core tube 3 and a breathable cotton 6.

[0041] The cylinder 1 is hollow inside and open at one end, and the interior is used to hold the bacteria. The cylinder 1 is made of glass, which has the advantages of stable chemical properties and convenient observation of the development of the bacteria. The size of the cylinder 1 can be adjusted as needed.

[0042] The end cap 2 is detachably connected to the open end of the cylinder 1, and the detachable connection method can be in the form of a threaded connection or a snap connection; the end cap 2 has a through hole 21 connected to the interior of the cylinder 1, and a connector 4 is inserted in the through hole 21; the connector 4 extends to the outside of the through hole 21 away from the cylinder 1, and the protruding end of the connector 4 is used to connect to the oxygen generator 8.

[0043] The gas-uniform core tube 3 is arranged inside the cylinder 1, with its axial direction parallel to the opening direction of the cylinder 1, and one end of the gas-uniform core tube 3 is rotatably connected to the end cover 2 and communicated with the through hole 21; a plurality of gas outlets are provided on the outer peripheral wall of the gas-uniform core tube 3, and the positions of the plurality of gas outlets are evenly distributed inside the cylinder 1, so as to ensure that oxygen is evenly discharged into the cylinder 1 through the plurality of gas outlets.

[0044] The breathable cotton 6 is arranged in the through hole 21 and is located between the joint 4 and the uniform air core tube 3; the breathable cotton 6 can adopt high-density breathable cotton 6, which can ensure the gas permeability while ensuring the function of preventing miscellaneous bacteria from entering the interior of the cylinder 1, thereby weakening the barrier effect of the breathable cotton 6.

[0045] When the joint 4 is connected to the oxygen generator 8 , the oxygen generated by the oxygen generator 8 can pass through the breathable cotton 6 and be input into the interior of the cylinder 1 through each air outlet.

[0046] In the embodiment of the present application, after the cylinder 1 is inoculated with the bacteria, the end cap 2 is installed at the open end of the cylinder 1. The end cap 2 is provided with a connector 4 for connecting to the oxygen generator 8, and oxygen can be input into the interior of the uniform air core tube 3 through the connector 4; the uniform air core tube 3 is arranged inside the cylinder 1 and is rotatably connected to the end cap 2; the outer peripheral wall of the uniform air core tube 3 is provided with a plurality of air outlets, and oxygen can be evenly discharged to various positions inside the cylinder 1 through the plurality of air outlets, so that the oxygen concentration inside the cylinder 1 remains consistent; the breathable cotton 6 between the connector 4 and the end cap 2 can prevent miscellaneous bacteria from entering the interior of the cylinder 1 while also reducing the barrier effect on oxygen, making oxygen flow smoother compared to the cotton plug in the prior art.

[0047] Compared with the prior art, the strain culture container provided in the embodiment of the present application has a smoother oxygenation process when strain culture is performed using the container, and can evenly disperse the oxygen introduced into the culture container.

[0048] In some embodiments, please refer to Figures 1 to 5As a specific embodiment of the bacterial culture container provided in the present application, multiple air outlets are arranged at intervals along the axial direction of the uniform air core tube 3; each air outlet is a plurality of air outlet pipes 32 arranged at intervals along the circumferential direction of the uniform air core tube 3, and each air outlet pipe 32 is connected to the interior of the uniform air core tube 3; the oxygen introduced into the interior of the uniform air core tube 3 can be discharged into the interior of the cylinder 1 through the multiple air outlet pipes 32.

[0049] By adopting the above technical solution, compared with the prior art, oxygen can have more outlets, and the multiple outlets are evenly arranged along the axial direction of the cylinder 1. The oxygen entering the uniform air core tube 3 can be discharged into the interior of the cylinder 1 through multiple outlet pipes 32, so that the oxygen concentration inside the cylinder 1 is more uniform.

[0050] In some embodiments, please refer to Figures 3 to 5 As a specific embodiment of the bacterial culture container provided in the present application, the air outlet pipe 32 includes a straight pipe section 321 and a turning section 322 .

[0051] The straight tube section 321 is disposed on the outer wall of the gas-uniform core tube 3 , and extends outward in the radial direction of the gas-uniform core tube 3 .

[0052] One end of the turning section 322 is connected to the end of the straight tube section 321 facing away from the gas-uniform core tube 3, and the other end is oriented perpendicularly to the axial direction of the straight tube section 321; the axial direction of the turning section 322 is perpendicular to the axial direction of the gas-uniform core tube 3, and the gas outlet directions of the turning sections 322 on multiple gas outlet pipes 32 on the same gas outlet portion are oriented in the same tangent direction along the circumference of the gas-uniform core tube 3.

[0053] By adopting the above technical solution, since the gas-uniforming core tube 3 is rotatably connected to the end cover 2, and the gas outlet direction of the changing section 322 is toward the same tangential direction, when oxygen is discharged from the gas outlets of multiple gas outlet pipes 32, the gas-uniforming core tube 3 can be subjected to a reaction force in the tangential direction, thereby causing the gas-uniforming core tube 3 to rotate, so that the oxygen is evenly input to various places inside the cylinder 1.

[0054] In some embodiments, please refer to Figure 3 and Figure 4 As a specific embodiment of the bacterial culture container provided in the present application, the end cover 2 has a groove 24 connected to the through hole 21 on the side facing the cylinder 1, the gas uniforming core tube 3 is embedded in the groove 24, and has a ring platform 31 extending radially outward thereon; and the end cover 2 is also detachably connected to a ring-shaped fixing plate 5.

[0055] When the annular platform 31 is embedded in the groove 24 and the end cover 2 is connected to the fixing plate 5 , the fixing plate 5 surrounds the gas-distributing core tube 3 and abuts against the annular platform 31 to limit the gas-distributing core tube 3 from moving away from the end cover 2 .

[0056] When installing the gas-uniform core tube 3, first put the fixing plate 5 on the gas-uniform core tube 3, then embed the ring platform 31 into the groove 24, and finally fix the mounting plate to the end cover 2; wherein, the thickness of the ring platform 31 is slightly smaller than the depth of the groove 24, and the outer diameter of the ring platform 31 is slightly smaller than the inner diameter of the groove 24, which provides space for the rotation of the gas-uniform core tube 3.

[0057] By adopting the above technical solution, the arrangement of the end cover 2 can realize the detachable installation of the gas-uniform core tube 3 , which provides convenience for the replacement and cleaning of the gas-uniform core tube 3 .

[0058] In some embodiments, please refer to Figures 3 to 5 As a specific embodiment of the bacterial culture container provided in the present application, the fixing plate 5 has a notch 51 for the air outlet to pass through.

[0059] By adopting the above technical solution, since the gas-uniform core tube 3 has a plurality of gas outlet pipes 32 , the fixing plate 5 with the notch 51 can be sleeved on the gas-uniform core tube 3 to avoid the plurality of gas outlet pipes 32 .

[0060] In some embodiments, see Figure 4 As a specific embodiment of the bacterial culture container provided in the present application, a plurality of thread grooves 22 are provided at the bottom of the groove 24; a plurality of alignment holes 52 corresponding to the plurality of thread grooves 22 are provided on the fixing plate 5, and the fixing plate 5 also includes a plurality of fastening bolts 53 corresponding to the plurality of alignment holes 52; the fastening bolts 53 are made of stainless steel to prevent the fastening bolts 53 from oxidizing and contaminating the bacterial strains.

[0061] When the plurality of alignment holes 52 are connected to the plurality of thread grooves 22 in a one-to-one correspondence, each fastening bolt 53 passes through the corresponding alignment hole 52 and is threadedly connected to the corresponding thread groove 22 .

[0062] By adopting the above technical solution, the detachable installation between the mounting plate and the end cover 2 can be achieved, and the bolt connection has the advantages of simple structure and convenient disassembly.

[0063] In some embodiments, see Figure 3 As a specific embodiment of the bacterial culture container provided in the present application, the end of the end cover 2 facing the barrel 1 has a threaded sleeve 23 surrounding the outer circumference of the barrel 1 and threadedly connected to the barrel 1.

[0064] By adopting the above technical scheme, the fixing form of the threaded connection can realize the detachable installation between the end cover 2 and the cylinder 1, and the threaded connection has the advantages of simple structure and easy disassembly; after the inoculation of the cylinder 1 is completed, the opening of the cylinder 1 can be quickly sealed to prevent miscellaneous bacteria from entering the cylinder 1 through the opening of the cylinder 1.

[0065] In some embodiments, please refer to Figure 3 and Figure 4 As a specific embodiment of the bacterial culture container provided in the present application, a sealing gasket 7 is further provided between the end cover 2 and the cylinder body 1; when the end cover 2 is connected to the cylinder body 1, the two side surfaces of the sealing gasket 7 are respectively in contact with the end cover 2 and the cylinder body 1 to block the gap between the end cover 2 and the cylinder body 1.

[0066] By adopting the above technical solution, the sealing gasket 7 is made of rubber or silicone material, which can prevent bacteria and external water vapor from entering the interior of the cylinder 1 through the gap between the end cover 2 and the cylinder 1.

[0067] In some embodiments, please refer to Figures 1 to 4 As a specific embodiment of the bacterial culture container provided in the present application, the outer wall of the joint 4 has a plurality of annular grooves 41 arranged at intervals along the axial direction of the cylinder 1; the joint 4 adopts a conical tube structure, and the outer diameter of the joint 4 gradually decreases from the end cover 2 to the outside, so that the joint 4 can be easily inserted into the connecting pipe of the oxygen generator 8.

[0068] By adopting the above technical solution, the multiple annular grooves 41 can ensure that when the connector 4 is connected to the connecting pipe of the oxygen generator 8, the friction between the outer wall of the connector 4 and the connecting pipe is increased, so that the connecting pipe is not easy to fall off.

[0069] The technical solution adopted in the present application also provides a constant temperature incubator, including any of the aforementioned bacterial culture containers.

[0070] The beneficial effects of the constant temperature incubator provided in this embodiment are the same as those of the aforementioned bacterial culture container, and will not be repeated here.

[0071] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A bacterial culture container, characterized in that: include: The cylinder body is hollow inside and open at one end, and the inside is used to hold the bacteria; An end cap is detachably connected to the open end of the cylinder, and has a through hole communicating with the interior of the cylinder, and a connector is inserted into the through hole; the connector extends to the outside of the through hole away from the cylinder, and the protruding end of the connector is used to connect to the oxygen concentrator; an air-uniform core tube, arranged inside the cylinder, with its axial direction parallel to the opening of the cylinder, and one end of which is rotatably connected to the end cover and communicated with the through hole; a plurality of air outlets are provided on the outer peripheral wall of the air-uniform core tube; and Breathable cotton, arranged in the through hole, and located between the joint and the air-uniform core tube; Wherein, when the joint is connected to the oxygen generator, the oxygen generated by the oxygen generator can pass through the breathable cotton and be input into the interior of the cylinder through each of the air outlets.

2. The bacterial culture container according to claim 1, characterized in that: The plurality of air outlets are arranged at intervals along the axial direction of the air-leveling core tube; each of the air outlets is a plurality of air outlet pipes arranged at intervals along the circumferential direction of the air-leveling core tube, and each of the air outlet pipes is connected to the interior of the air-leveling core tube.

3. The bacterial culture container according to claim 2, characterized in that: The air outlet pipe comprises: a straight tube section, which is arranged on the outer wall of the gas-uniform core tube and extends outward in the radial direction of the gas-uniform core tube; and The direction-changing section has one end connected to one end of the straight tube section facing away from the gas-uniform core tube, and the other end thereof is oriented perpendicular to the axial direction of the straight tube section.

4. The bacterial culture container according to claim 1, characterized in that: The end cover has a groove connected to the through hole on one side facing the cylinder, the gas-uniform core tube is embedded in the groove, and has a ring platform extending radially outward; and the end cover is detachably connected to a ring-shaped fixing plate; When the annular platform is embedded in the groove and the end cover is connected to the fixing plate, the fixing plate is arranged around the gas-distributing core tube, and the fixing plate abuts against the annular platform to limit the gas-distributing core tube from moving away from the end cover.

5. The bacterial culture container according to claim 4, characterized in that: The fixing plate has a slot for the air outlet to pass through.

6. The bacterial culture container according to claim 4, characterized in that: The groove bottom of the groove is provided with a plurality of thread grooves; the fixing plate is provided with a plurality of alignment holes corresponding to the plurality of thread grooves in one-to-one communication, and the fixing plate further comprises a plurality of fastening bolts corresponding to the plurality of alignment holes in one-to-one communication; When the plurality of alignment holes are connected to the plurality of thread grooves in a one-to-one correspondence, each of the fastening bolts passes through the corresponding alignment hole and is threadedly connected to the corresponding thread groove.

7. The bacterial culture container according to claim 1, characterized in that: One end of the end cover facing the cylinder is provided with a threaded sleeve surrounding the outer periphery of the cylinder and threadedly connected with the cylinder.

8. The bacterial culture container according to claim 1, characterized in that: A sealing gasket is also provided between the end cover and the cylinder; when the end cover is connected to the cylinder, two side surfaces of the sealing gasket abut against the end cover and the cylinder respectively to block the gap between the end cover and the cylinder.

9. The bacterial culture container according to claim 1, characterized in that: The outer wall of the joint is provided with a plurality of annular grooves which are arranged at intervals along the axial direction of the cylinder.

10. Constant temperature incubator, characterized in that: A bacterial culture container comprising any one of claims 1 to 9.

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