Superparamagnetic immunomagnetic bead automatic adsorption and separation device based on bistable magnets

Abstract

The utility model relates to the technical field of biomedicine, in particular to a superparametric immunomagnetic bead automatic adsorption and separation device based on a bistable magnet, which is characterized in that a plurality of bulges are fixed on the outer side of a shell, and a magnetic bead solution placing pipe frame is arranged on the bulges; the moving magnet assembly is slidably connected in the shell and moves up and down along the inner wall of the shell; the electromagnet assembly is arranged in the shell, fixed to the lower side of the moving magnet assembly and has a switch-on state and a switch-off state, and in the switch-on state, the electromagnet assembly generates a magnetic field and destroys the adsorption magnetic field of the moving magnet assembly to adsorb magnetic beads in the magnetic bead solution containing pipe frame; in a closed state, the moving magnet assembly normally attracts magnetic beads in the magnetic bead solution placing pipe frame; the power supply structure is fixed to the side, away from the moving magnet assembly, of the electromagnet assembly, connected with the electromagnet assembly and the moving magnet assembly, controls the state of the electromagnet assembly and controls the moving magnet assembly to move up and down. The magnetic attraction process is simplified, the operation is simple, and the use is quick.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedicine, in particular to a superparamagnetic immune bead automatic adsorption and separation device based on a bistable magnet. Background Art

[0002] Immunoassay is a biochemical detection method based on the principle of antigen-antibody reaction, which is widely used in medicine, biology, environmental science and other fields. Superparamagnetic immunomagnetic beads are often used in immunoassay. Superparamagnetic immunomagnetic beads are microparticles composed of a magnetic core, a polymer coating and a functional base layer. They can quickly separate and enrich target biomolecules or cells under the action of an external magnetic field. Superparamagnetic immunomagnetic beads have the advantages of high specific surface area, high affinity, high sensitivity and high selectivity, which can improve the efficiency and accuracy of immunoassay.

[0003] The commonly used immunomagnetic bead separation method in the laboratory is to use a magnet on a small magnetic frame to adsorb magnetic beads, but this method is limited by the strength of the magnet, requires waiting for 1 to 2 minutes, and may reduce the recovery rate and repeatability of the magnetic beads. Therefore, it is necessary to design a super-parallel immunomagnetic bead automatic adsorption and separation device based on a bistable magnet to solve the above problems. Utility Model Content

[0004] The utility model aims to provide a superparamagnetic immunomagnetic bead automatic adsorption and separation device based on a bistable magnet to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above-mentioned purpose, the utility model provides the following scheme: The utility model provides a superparamagnetic immunomagnetic bead automatic adsorption and separation device based on a bistable magnet, comprising:

[0006] The outer side of the shell is fixed with a plurality of protrusions, and the protrusions are provided with a tube rack for placing the magnetic bead solution;

[0007] A moving magnet assembly is slidably connected in the shell and moves up and down along the inner wall of the shell;

[0008] The electromagnet assembly is arranged in the housing and fixed on the lower side of the moving magnet assembly, and has an on state and an off state. In the on state, the electromagnet assembly generates a magnetic field to destroy the adsorption magnetic field of the moving magnet assembly to adsorb the magnetic beads in the tube rack for placing the magnetic bead solution; in the off state, the moving magnet assembly normally attracts the magnetic beads in the tube rack for placing the magnetic bead solution;

[0009] The power supply structure is fixed on the side of the electromagnet assembly away from the moving magnet assembly, connected to the electromagnet assembly and the moving magnet assembly, and controls the state of the electromagnet assembly and controls the moving magnet assembly to move up and down.

[0010] Preferably, the moving magnet assembly comprises:

[0011] a pair of guide rails fixed on the inner wall of the housing;

[0012] A pair of sliders are fixed on the outer side of a permanent magnet. The sliders are slidably connected to the guide rails. A driving member is also fixed on the sliders. The driving member drives the sliders to move in the guide rails.

[0013] Preferably, the driving member comprises:

[0014] A driving motor is fixed on the slider;

[0015] A gear is fixed on the driving end of the driving motor, a rack is arranged on the guide rail, and the gear is meshed with the rack.

[0016] Preferably, the electromagnet assembly comprises:

[0017] The iron core is fixed in the shell and has a coil wound around it.

[0018] Preferably, the power supply structure includes:

[0019] A power supply coil is arranged at the lower side of the iron core and is wound around and attached to the inner wall of the shell;

[0020] A power supply is connected to the power supply coil and is arranged outside the shell. A switch is also arranged between the power supply coil and the power supply.

[0021] Preferably, the power supply coil is connected to the coil and the drive motor respectively, the switch controls the connection or disconnection between the coil and the power supply, and the switch also controls the vertical movement of the permanent magnet.

[0022] Preferably, the magnetic bead solution placement tube rack comprises:

[0023] The magnetic bead solution collection rack has a hanging plate fixed on one side close to the shell, and the hanging plate is snap-fitted and adapted to the protrusion; the magnetic bead solution collection rack also has a plurality of buckles fixed on the side away from the shell;

[0024] The collection tube is fixed in the magnetic bead solution collection rack by a snap.

[0025] The utility model discloses the following technical effects:

[0026] The utility model simplifies the magnetic absorption process, and the magnetic bead absorption process can be completed by the coordinated use of the power supply structure and the electromagnet assembly. During operation, the moving magnet assembly can be moved up and down by controlling the corresponding part of the power supply structure, so that the magnetic beads in the tube rack of the magnetic bead solution can be quickly gathered, and the magnetic beads can be quickly dispersed by the electromagnet assembly. The operation is simple and the use is fast, which reduces the problem of repeatability and accuracy of immunodiagnosis caused by the decrease in magnetic bead recovery rate due to the low magnetic attraction of the traditional magnetic frame. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0029] Figure 2 It is the relationship diagram between the housing and the permanent magnet position;

[0030] Figure 3 is the relationship diagram between the permanent magnet and the coil position;

[0031] Figure 4 for Figure 2 The enlarged schematic diagram of part A in the middle;

[0032] Among them, 1. a tube rack for placing a magnetic bead solution; 11. a magnetic bead solution collecting rack; 12. a hanging plate; 13. a buckle; 14. a collecting tube; 2. a shell; 21. a protrusion; 22. a groove; 3. a moving magnet assembly; 31. a guide rail; 32. a slider; 33. a permanent magnet; 34. a gear; 35. a driving motor; 331. a magnetic surface; 4. an electromagnet assembly; 41. a coil; 42. an iron core; 5. a power supply structure; 51. a power supply coil; 52. a switch; 53. a power supply. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0034] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0035] Reference Figure 1-Figure 4 The utility model discloses a super-parallel immune magnetic bead automatic adsorption and separation device based on a bistable magnet, a shell 2, a plurality of protrusions 21 are fixed on the outer side, and a magnetic bead solution placement tube rack 1 is arranged on the protrusion 21; a moving magnet component 3 is slidably connected in the shell 2 and moves up and down along the inner wall of the shell 2; an electromagnet component 4 is arranged in the shell 2 and fixed on the lower side of the moving magnet component 3, and has an on state and an off state. In the on state, the electromagnet component 4 generates a magnetic field to destroy the adsorption magnetic field of the moving magnet component 3 to adsorb the magnetic beads in the magnetic bead solution placement tube rack 1; in the off state, the moving magnet component 3 normally attracts the magnetic beads in the magnetic bead solution placement tube rack 1; a power supply structure 5 is fixed on the side of the electromagnet component 4 away from the moving magnet component 3, is connected to the electromagnet component 4 and the moving magnet component 3, and controls the state of the electromagnet component 4 and controls the moving magnet component 3 to move up and down.

[0036] Specifically, the power supply structure 5 controls the moving magnet assembly 3 to move up and down, so that the moving magnet assembly 3 can normally attract the magnetic beads in the magnetic bead solution placement tube rack 1 at the specified position; when the electromagnet assembly 4 is in the closed state, the electromagnet assembly 4 does not generate a magnetic field that interferes with the moving magnet assembly 3, and the moving magnet assembly 3 can normally attract the magnetic beads in the magnetic bead solution placement tube rack 1; when it is in the on state, the electromagnet assembly 4 generates a magnetic field that interferes with the adsorption magnetic field of the moving magnet assembly 3, so that the magnetic beads in the magnetic bead solution placement tube rack 1 can be quickly dispersed.

[0037] The device simplifies the magnetic absorption process, and the magnetic bead absorption process can be completed by the coordinated use of the power supply structure 5 and the electromagnet assembly 4. During operation, the moving magnet assembly 3 can be moved up and down by controlling the corresponding part of the power supply structure 5, so that the magnetic beads in the magnetic bead solution tube rack 1 are quickly gathered, and the magnetic beads are quickly dispersed by the electromagnet assembly. The operation is simple and the use is fast, which reduces the problem of repeatability and accuracy of immunodiagnosis caused by the decrease in magnetic bead recovery rate due to the low magnetic attraction of the traditional magnetic rack.

[0038] In addition, the shell 2 is made of polyphenylene sulfide (PPS), which has high high temperature resistance and insulation properties.

[0039] The moving magnet assembly 3 includes: a guide rail 31 fixed on the inner wall of the shell 2; a permanent magnet 33, with a slider 32 fixed on the outside, the slider 32 is slidably connected in the guide rail 31, and the slider 32 is also fixed with a driving member, which drives the slider 32 to move on the guide rail 31.

[0040] It should be noted that the permanent magnet 33 is provided with a magnetic attraction surface 331 with the strongest magnetism, and the magnetic attraction surface 331 can generate a strong adsorption magnetic field so that the superparamagnetic immune beads are adsorbed at a designated position.

[0041] The driving member includes: a driving motor 35 fixed on the slider 32; a gear 34 fixed on the driving end of the driving motor 35, a rack is provided on the guide rail 31, and the gear 34 is meshed with the rack.

[0042] The driving motor 35 can rotate forward and reverse, so that the gear 34 can drive the slider 32 to move upward or downward along the guide rail 31 .

[0043] The electromagnet assembly 4 includes an iron core 42 fixed in the housing 2 and having a coil 41 wound around the outside.

[0044] Among them, the coil 41 on the electromagnet assembly 4 mainly generates an interfering magnetic field when releasing the magnetic beads, so that the magnetic beads in the collection tube 14 are scattered under the interfering magnetic field, and the superparamagnetic immune magnetic beads are released into the storage solution in the collection tube 14, thereby realizing the recovery of the magnetic beads.

[0045] The power supply structure 5 includes: a power supply coil 51, which is arranged on the lower side of the iron core 42, wound around and attached to the inner wall of the shell 2; a power supply 53, which is connected to the power supply coil 51 and arranged outside the shell 2, and a switch 52 is also arranged between the power supply coil 51 and the power supply 53.

[0046] The power source 53 may be a common battery. When the power source 53 runs out of power, it may be directly connected to an indoor circuit via wires. The corresponding power supply method may be selected according to the actual application.

[0047] The power supply coil 51 is connected to the coil 41 and the drive motor 35 respectively, and the switch 52 controls the connection or disconnection between the coil 41 and the power supply 53. The switch 52 also controls the up and down movement of the permanent magnet 33. The switch 52 controls the slider 32 to move up or down on the guide rail 31 by controlling the drive shaft of the drive motor 35 to rotate forward or reverse.

[0048] The switch 52 is provided with a button for controlling the forward or reverse rotation of the driving motor 35 , so that the gear 34 can be controlled to rotate forward or reverse by different buttons, thereby controlling the slider 32 to move upward or downward on the guide rail 31 .

[0049] The magnetic bead solution collection rack 11 is fixed with a hanging plate 12 on the side close to the shell 2, and the hanging plate 12 is snap-fitted with and adapted to the protrusion 21; the magnetic bead solution collection rack 11 is also fixed with a plurality of buckles 13 on the side away from the shell 2; the collection tube 14 is fixed in the magnetic bead solution collection rack 11 through the buckles 13.

[0050] Among them, the buckle 13 is made of elastic material, and a hanging hole is opened on the hanging plate 12, which is adapted to the protrusion 21. The hanging plate 12 is connected to the protrusion 21 by snapping, and at the same time, the buckle 13 snaps the collection tube 14 into the magnetic bead solution collection rack 11 to ensure that the magnetic bead solution collection rack 11 and the collection tube 14 will not fall off.

[0051] Working process: First, before the immune reaction, the solution mixed with super-parallel immune magnetic beads and target biological molecules or cells is loaded into the collection tube 14, and the collection tube 14 is placed in each magnetic bead solution collection rack 11, and is limited by the plastic buckle 13. Then, the collection tube 14 and the hanging plate 12 are snapped on the cylindrical protrusion 21 on the shell 2, and then, the switch 52 is turned on and the drive motor 35 is started to move the slider 32 downward along the up and down motion guide rail 31. When the slider 32 moves to the lowest position, the magnetic absorption surface 331 of the permanent magnet 33 is aligned with the bottom of each collection tube 14, and a strong adsorption magnetic field is generated, so that the super-parallel immune magnetic beads are adsorbed on the bottom of the collection tube 14, thereby realizing the separation of the magnetic beads. At this time, the upper end of the collection tube 14 can be opened, the solution in the collection tube 14 can be taken out, and then the magnetic bead storage solution is dripped in to assist in the storage of the magnetic beads, and the internal solution is replaced to carry out the next step of the magnetic bead operation. When the magnetic beads need to be released, the coil 41 is turned on and energized. At this time, the coil 41 generates a magnetic field, destroys the adsorption magnetic field of the permanent magnet 33, and releases the adsorbed magnetic beads. At the same time, the drive motor 35 is controlled to drive the gear 34 to move upward and drive the permanent magnet 33 to move upward, so that the superparamagnetic immune magnetic beads are released into the solution in the collection tube 14, thereby realizing the recovery of the magnetic beads.

[0052] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0053] The embodiments described above are only descriptions of the preferred methods of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope determined by the claims of the present invention.

Claims

1. A superparamagnetic immunomagnetic bead automatic adsorption and separation device based on a bistable magnet, characterized in that: include: The outer side of the housing (2) is fixed with a plurality of protrusions (21), and a tube rack (1) for placing a magnetic bead solution is arranged on the protrusions (21); A moving magnet assembly (3) is slidably connected in the housing (2) and moves up and down along the inner wall of the housing (2); The electromagnet assembly (4) is arranged in the housing (2) and fixed on the lower side of the moving magnet assembly (3), and has an on state and an off state. In the on state, the electromagnet assembly (4) generates a magnetic field to destroy the adsorption magnetic field of the moving magnet assembly (3) to adsorb the magnetic beads in the magnetic bead solution placement tube rack (1); in the off state, the moving magnet assembly (3) normally attracts the magnetic beads in the magnetic bead solution placement tube rack (1); The power supply structure (5) is fixed on the side of the electromagnet assembly (4) away from the moving magnet assembly (3), connected to the electromagnet assembly (4) and the moving magnet assembly (3), and controls the state of the electromagnet assembly (4) and controls the upward and downward movement of the moving magnet assembly (3).

2. The superparamagnetic immunomagnetic bead automatic adsorption and separation device based on a bistable magnet according to claim 1, characterized in that: The moving magnet assembly (3) comprises: A pair of guide rails (31) fixed on the inner wall of the housing (2); A pair of sliders (32) are fixed on the outside of the permanent magnet (33), the sliders (32) are slidably connected to the guide rail (31), and a driving member is also fixed to the slider (32), and the driving member drives the slider (32) to move in the guide rail (31).

3. The superparamagnetic immunomagnetic bead automatic adsorption and separation device based on a bistable magnet according to claim 2, characterized in that: The driving member comprises: A driving motor (35) fixed on the slider (32); A gear (34) is fixed to the driving end of the driving motor (35), a rack is provided on the guide rail (31), and the gear (34) is meshed with the rack.

4. The superparamagnetic immunomagnetic bead automatic adsorption and separation device based on a bistable magnet according to claim 2, characterized in that: The electromagnet assembly (4) comprises: The iron core (42) is fixed in the housing (2) and has a coil (41) wound around it.

5. The superparamagnetic immunomagnetic bead automatic adsorption and separation device based on a bistable magnet according to claim 4, characterized in that: The power supply structure (5) comprises: A power supply coil (51) is arranged on the lower side of the iron core (42), and is wound around and attached to the inner wall of the housing (2); A power source (53) is connected to the power supply coil (51) and is arranged outside the housing (2); a switch (52) is also arranged between the power supply coil (51) and the power source (53).

6. The superparamagnetic immunomagnetic bead automatic adsorption and separation device based on a bistable magnet according to claim 5, characterized in that: The power supply coil (51) is connected to the coil (41) and the drive motor (35) respectively, and the switch (52) controls the connection or disconnection between the coil (41) and the power supply (53). The switch (52) also controls the vertical movement of the permanent magnet (33).

7. The superparamagnetic immunomagnetic bead automatic adsorption and separation device based on a bistable magnet according to claim 1, characterized in that: The magnetic bead solution placement tube rack (1) comprises: A magnetic bead solution collection rack (11) is fixed with a hanging plate (12) on the side close to the housing (2), and the hanging plate (12) is snap-fitted and adapted to the protrusion (21); a plurality of buckles (13) are also fixed on the side of the magnetic bead solution collection rack (11) away from the housing (2); The collection tube (14) is fixed in the magnetic bead solution collection rack (11) via a buckle (13).

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