Sample incubation cleaning device for cracking elution instrument

Abstract

The utility model relates to the technical field of biomedical research, in particular to a sample incubation cleaning device for a cracking elution instrument. The sample incubation and cleaning device for the cracking elution instrument comprises a shell, a test tube, an incubation chamber and a cleaning assembly, wherein the test tube is used for accommodating a sample; the incubation chamber is arranged in the shell, and the test tube is arranged in the incubation chamber; the cleaning assembly comprises a cleaning head, a vertical moving structure, a horizontal moving structure and a cleaning structure, the cleaning structure is installed in the shell, one end of the vertical moving structure is connected with the cleaning head, the other end of the vertical moving structure penetrates through the shell to be connected with the horizontal moving structure, the cleaning head is used for cleaning a test tube, and the cleaning structure is arranged in the shell; the cleaning head can penetrate through the shell to be inserted into the cleaning structure which is used for cleaning the cleaning head. Compared with the prior art, through cooperation of the vertical moving structure and the horizontal moving structure, the cleaning head can be moved into the test tube to clean the test tube, the test tube does not need to be moved, the cleaning time is saved, and the experiment efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical research, in particular to a sample incubation and cleaning device for a lysis and elution instrument. Background Art

[0002] Flow cytometry is used to analyze and count the cell types, numbers, and functions in cell suspensions. It can simultaneously detect the expression of multiple cell surface and internal molecular markers by passing the cell suspension through a flow cytometer for rapid and continuous detection and analysis of single cells. Flow cytometry has a wide range of applications. It can be used for expression analysis of cell surface markers, such as cell membrane receptors, cell adhesion molecules, etc.; it can also be used for the detection of internal markers, such as intracellular proteins, nucleic acids, organelles, etc.; in addition, flow cytometry can also be used for cell sorting and purification. By setting a threshold for cells expressing specific markers, target cells can be separated.

[0003] However, in the prior art, the cleaning of the test tubes must be carried out on a cleaning table, requiring the experimenter to repeatedly move between the experimental equipment and the cleaning table, which consumes a lot of time, affects the experimental efficiency, and affects the timeliness of the experimental results.

[0004] Therefore, there is an urgent need for a sample incubation and cleaning device for a lysis and elution instrument to solve the above technical problems. Utility Model Content

[0005] The utility model aims to provide a sample incubation and cleaning device for a lysis and elution instrument, which can perform in-situ cleaning of test tubes, reduce cleaning time, and improve experimental efficiency.

[0006] To achieve this purpose, the utility model adopts the following technical solutions:

[0007] A sample incubation and cleaning device for a lysis and elution instrument comprises:

[0008] case;

[0009] a test tube for containing a sample;

[0010] an incubation chamber, wherein the incubation chamber is disposed in the housing, and the test tube is disposed in the incubation chamber;

[0011] A cleaning assembly, the cleaning assembly comprising a cleaning head, a vertical moving structure, a horizontal moving structure and a cleaning structure, the cleaning structure being installed in the shell, one end of the vertical moving structure being connected to the cleaning head, the other end of the vertical moving structure passing through the shell and connected to the horizontal moving structure, the cleaning head being used to clean the test tube, the cleaning head being able to pass through the shell and be inserted into the cleaning structure, and the cleaning structure being used to clean the cleaning head.

[0012] As an optimal technical solution of the above-mentioned sample incubation and cleaning device for the lysis and elution instrument, the cleaning head includes a mounting part and a plurality of cleaning needles, the mounting part is mounted on the vertical moving structure, the plurality of cleaning needles are evenly mounted on the mounting part, and the cleaning structure can clean the cleaning needles.

[0013] As a preferred technical solution of the sample incubation and cleaning device for the lysis and elution instrument, the sample incubation and cleaning device for the lysis and elution instrument further includes a heating component, and the heating component is installed in the incubation chamber.

[0014] As an optimal technical solution of the above-mentioned sample incubation and cleaning device for the lysis and elution instrument, the sample incubation and cleaning device for the lysis and elution instrument also includes an oscillation component, which is installed in the incubation chamber. The oscillation component includes an oscillation seat, an eccentric wheel and a first drive. The oscillation seat is connected to the eccentric part of the eccentric wheel, the eccentric wheel is connected to the output end of the first drive, and the test tube is arranged on the oscillation seat.

[0015] As an optimal technical solution of the above-mentioned sample incubation and cleaning device for the lysis and elution instrument, the sample incubation and cleaning device for the lysis and elution instrument also includes a magnetic frame assembly, which is installed in the incubation chamber and is located below the oscillation seat. The magnetic frame assembly includes a fixed frame, a plurality of magnets, a first guide rail and a second drive. The first guide rail is arranged in the incubation chamber, the fixed frame is slidably connected to the first guide rail, a plurality of magnets are fixedly installed on the fixed frame, and the output end of the second drive is connected to the fixed frame.

[0016] As an optimal technical solution for the above-mentioned sample incubation and cleaning device for the lysis and elution instrument, the horizontal moving structure includes a third drive, two first synchronous wheels and a first synchronous belt, the third drive is installed in the shell, the two first synchronous wheels are rotatably installed at both ends of the shell, the first synchronous belt is simultaneously sleeved with two first synchronous wheels, the output end of the third drive is connected to any one of the two first synchronous wheels, and the vertical moving structure is installed on the first synchronous belt.

[0017] As an optimal technical solution of the above-mentioned sample incubation and cleaning device for the lysis and elution instrument, the sample incubation and cleaning device for the lysis and elution instrument also includes a second guide rail, which is installed in the shell, and the vertical moving structure is slidably connected to the second guide rail, and the vertical moving structure can move along the extension direction of the second guide rail under the drive of the first synchronous belt.

[0018] As an optimal technical solution of the above-mentioned sample incubation and cleaning device for the lysis and elution instrument, the sample incubation and cleaning device for the lysis and elution instrument also includes two first limit members, and the two first limit members are respectively installed at the two ends of the second guide rail, and the vertical moving structure can respectively resist the two first limit members.

[0019] As an optimal technical solution for the above-mentioned sample incubation and cleaning device for the lysis and elution instrument, the vertical moving structure includes a third guide rail, a fourth drive, two second synchronous wheels and a second synchronous belt. The third guide rail is fixedly connected to the first synchronous belt, and the third guide rail is slidably connected to the second guide rail. The fourth drive is installed on the third guide rail, and the two second synchronous wheels are rotatably installed on the two ends of the third guide rail. The second synchronous belt is simultaneously sleeved with two second synchronous wheels. The output end of the fourth drive is connected to any one of the two second synchronous wheels, and the cleaning head is slidably connected to the third guide rail.

[0020] As an optimal technical solution of the above-mentioned sample incubation and cleaning device for the lysis and elution instrument, the sample incubation and cleaning device for the lysis and elution instrument also includes two second limit members, and the two second limit members are respectively installed at the two ends of the third guide rail, and the cleaning head can respectively abut against the two second limit members.

[0021] Beneficial effects of the utility model:

[0022] The utility model provides a sample incubation and cleaning device for a lysis and elution instrument. The sample incubation and cleaning device for a lysis and elution instrument comprises: a shell, a test tube, an incubation chamber and a cleaning component, wherein the test tube is used to hold the sample; the incubation chamber is arranged in the shell, and the test tube is arranged in the incubation chamber; the cleaning component comprises a cleaning head, a vertical moving structure, a horizontal moving structure and a cleaning structure, wherein the cleaning structure is installed in the shell, one end of the vertical moving structure is connected to the cleaning head, and the other end of the vertical moving structure passes through the shell and is connected to the horizontal moving structure, the cleaning head is used to clean the test tube, the cleaning head can pass through the shell and be inserted into the cleaning structure, and the cleaning structure is used to clean the cleaning head. Compared with the prior art, the cleaning head can be moved into the test tube through the cooperation of the vertical moving structure and the horizontal moving structure, and the test tube is cleaned without moving the test tube, thereby saving cleaning time and improving experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the contents of the embodiments of the present invention and these drawings without paying any creative work.

[0024] Figure 1 A schematic diagram of the structure of a sample incubation and cleaning device for a lysis and elution instrument provided in an embodiment of the utility model Figure 1 ;

[0025] Figure 2 A schematic diagram of the structure of a sample incubation and cleaning device for a lysis and elution instrument provided in an embodiment of the utility model Figure 2 ;

[0026] Figure 3 The structure of the oscillating assembly provided in the embodiment of the utility model is schematically shown in FIG. Figure 1 ;

[0027] Figure 4 The structure of the oscillating assembly provided in the embodiment of the utility model is schematically shown in FIG. Figure 2 ;

[0028] Figure 5 A schematic diagram of the structure of a magnetic frame assembly provided in an embodiment of the utility model;

[0029] Figure 6 A schematic diagram of the structure of a sample incubation and cleaning device for a lysis and elution instrument provided in an embodiment of the utility model Figure 3 .

[0030] In the figure:

[0031] 1. Shell;

[0032] 2. Test tube;

[0033] 3. incubation chamber; 31. through hole; 32. opening;

[0034] 4. Cleaning head; 41. Mounting piece; 42. Cleaning needle;

[0035] 5. vertical moving structure; 51. third guide rail; 52. fourth drive; 53. second synchronous wheel; 54. second synchronous belt;

[0036] 6. Horizontal moving structure; 61. Third drive; 62. First synchronous wheel; 63. First synchronous belt;

[0037] 7. Clean the structure;

[0038] 8. Heating component;

[0039] 9. Oscillation assembly; 91. Oscillation seat; 92. Eccentric wheel; 93. First drive; 94. Third synchronous wheel; 95. Fourth synchronous wheel; 96. Fifth synchronous wheel; 97. Third synchronous belt;

[0040] 10. Magnetic frame assembly; 101. Fixed frame; 102. Magnet; 103. First guide rail; 104. Second drive;

[0041] 11. Second guide rail;

[0042] 12. Support column;

[0043] 13. Shading cover;

[0044] 14. Test tube rack;

[0045] 15. Fifth drive;

[0046] 16. Sixth synchronous wheel;

[0047] 17. Fourth synchronous belt. DETAILED DESCRIPTION

[0048] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It should also be noted that, for ease of description, only the parts related to the present invention, rather than all structures, are shown in the accompanying drawings.

[0049] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0050] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0051] In the description of this embodiment, the terms "upper", "lower", "left", "right" and other directions or positional relationships are based on the directions or positional relationships shown in the drawings, and are only for the convenience of description and simplified operation, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first" and "second" are only used to distinguish in the description and have no special meaning.

[0052] like Figure 1 As shown, the utility model provides a sample incubation and cleaning device for a lysis and elution instrument. The sample incubation and cleaning device for a lysis and elution instrument comprises: a housing 1, a test tube 2, an incubation chamber 3 and a cleaning component.

[0053] Specifically, the test tube 2 is used to contain the sample, and the sample is incubated in the test tube 2; the incubation chamber 3 is arranged in the shell 1, and the test tube 2 is arranged in the incubation chamber 3; the cleaning assembly includes a cleaning head 4, a vertical moving structure 5, a horizontal moving structure 6 and a cleaning structure 7, the cleaning structure 7 is installed in the shell 1, one end of the vertical moving structure 5 is connected to the cleaning head 4, and the other end of the vertical moving structure 5 passes through the shell 1 and is connected to the horizontal moving structure 6, the cleaning head 4 is used to clean the test tube 2, the cleaning structure 7 is arranged in the shell 1, the cleaning head 4 can pass through the shell 1 and be inserted into the cleaning structure 7, and the cleaning structure 7 is used to clean the cleaning head 4. Compared with the prior art, the cleaning head 4 can be moved into the test tube 2 by the cooperation of the vertical moving structure 5 and the horizontal moving structure 6 to clean the test tube 2, and the test tube 2 does not need to be moved, which saves cleaning time and improves experimental efficiency; and after the test tube 2 is cleaned, the vertical moving structure 5 and the horizontal moving structure 6 cooperate to move the cleaning head 4 to the cleaning structure 7 to clean the cleaning head 4.

[0054] Preferably, if Figure 1 and Figure 2 As shown, the cleaning head 4 includes a mounting member 41 and a plurality of cleaning needles 42, the mounting member 41 is mounted on the vertical moving structure 5, the plurality of cleaning needles 42 are evenly mounted on the mounting member 41, and the cleaning needles 42 can be inserted into the cleaning structure 7 so that the cleaning structure 7 cleans the cleaning needles 42. Specifically, the cleaning head 4 is provided with a mounting member 41 and a plurality of cleaning needles 42, the plurality of cleaning needles 42 are evenly mounted on the mounting member 41, and a plurality of test tubes 2 can be cleaned at the same time, thereby improving the cleaning efficiency; the cleaning structure 7 is provided with a plurality of cleaning chambers, and the plurality of cleaning needles 42 are arranged one by one corresponding to the plurality of cleaning chambers, and a plurality of cleaning needles 42 can be cleaned at the same time, thereby improving the cleaning efficiency.

[0055] Optionally, in this embodiment, 40 cleaning needles 42 are provided, and 40 test tubes 2 are provided, so that batch cleaning of the test tubes 2 can be performed. Of course, in some other embodiments, the number of cleaning needles 42 is determined according to the number of test tubes 2, which will not be repeated here.

[0056] Preferably, in order to increase the incubation speed of the sample, the sample incubation and cleaning device for the lysis and elution instrument also includes a heating component 8, which is installed in the incubation chamber 3 and can heat the incubation chamber 3 to maintain the temperature range of the incubation chamber 3 at 37°C±1°C, thereby accelerating the incubation of the sample.

[0057] Preferably, if Figure 3 and Figure 4 As shown, the sample incubation and cleaning device for the lysis and elution instrument further includes an oscillation assembly 9, which is installed in the incubation chamber 3. The oscillation assembly 9 includes an oscillation seat 91, an eccentric wheel 92 and a first drive 93. The oscillation seat 91 is connected to the eccentric part of the eccentric wheel 92, and the eccentric wheel 92 is connected to the output end of the first drive 93. The test tube 2 is arranged on the oscillation seat 91. Specifically, the sample incubation and cleaning device for the lysis and elution instrument is provided with an oscillation assembly 9, which is arranged in the incubation chamber 3 and is used to oscillate the test tube 2 so that the liquid in the test tube 2 is mixed evenly; wherein, the oscillation assembly 9 includes an oscillation seat 91, an eccentric wheel 92 and a first drive 93, the eccentric wheel 92 is rotatably installed in the incubation chamber 3, the first drive 93 is transmission-connected to the eccentric wheel 92, and the oscillation seat 91 is connected to the eccentric part of the eccentric wheel 92, so that the eccentric wheel 92 rotates to drive the oscillation seat 91 to reciprocate, and the test tube 2 is arranged on the oscillation seat 91, so that the liquid in the test tube 2 can be fully mixed.

[0058] Optionally, the oscillation assembly 9 further includes a third synchronous wheel 94, a fourth synchronous wheel 95, a fifth synchronous wheel 96 and a third synchronous belt 97, the eccentric wheel 92, the third synchronous wheel 94, the fourth synchronous wheel 95 and the fifth synchronous wheel 96 are arranged at intervals and staggered, the third synchronous belt 97 connects the eccentric wheel 92, the third synchronous wheel 94, the fourth synchronous wheel 95 and the fifth synchronous wheel 96, and the first drive 93 is connected to any one of the third synchronous wheel 94, the fourth synchronous wheel 95 and the fifth synchronous wheel 96, so that the oscillation seat 91 can move stably. In this embodiment, the first drive 93 is connected to the fifth synchronous wheel 96. Of course, in some other embodiments, the first drive 93 can be connected to the third synchronous wheel 94, and the first drive 93 can also be connected to the fourth synchronous wheel 95.

[0059] Preferably, the sample incubation and cleaning device for the lysis and elution instrument also includes four support columns 12, which are respectively arranged at the four corners of the oscillation base 91, and can effectively support the oscillation base 91 to prevent the oscillation base 91 from tilting; and, in order not to affect the movement of the oscillation base 91, the support columns 12 are made of elastic material and can be deformed to avoid affecting the movement of the oscillation base 91. For example, the support columns 12 can be made of rubber, silicone, etc., which will not be repeated here.

[0060] Preferably, if Figure 5As shown, the sample incubation and cleaning device for the lysis and elution instrument also includes a magnetic frame assembly 10, which is installed in the incubation chamber 3 and is located below the oscillation seat 91. The magnetic frame assembly 10 includes a fixed frame 101, a plurality of magnets 102, a first guide rail 103 and a second drive 104. The fixed frame 101 is slidably connected to the first guide rail 103, the plurality of magnets 102 are fixedly installed on the fixed frame 101, and the output end of the second drive 104 is connected to the fixed frame 101. Specifically, the first guide rail 103 is vertically installed in the incubation chamber 3 and is located below the oscillation seat 91. The fixed frame 101 is slidably installed on the first guide rail 103. A plurality of magnets 102 are installed on the fixed frame 101 at intervals. The second drive 104 can drive the fixed frame 101 to move along the first guide rail 103, so that the plurality of magnets 102 are close to or away from the bottom of the test tube 2. When the plurality of magnets 102 are close to the bottom of the test tube 2, the magnets 102 can adsorb the magnetic microspheres in the test tube 2 and make them adhere to the side wall of the test tube 2 for easy purification.

[0061] It should be noted that, illustratively, when in the incubation oscillation process, the oscillation component 9 works to drive the test tube 2 to oscillate. At this time, in order to fully mix the liquid in the test tube 2, the magnetic frame component 10 does not work, and the plurality of magnets 102 are located away from the bottom of the test tube 2 to prevent the plurality of magnets 102 from adsorbing the magnetic microspheres in the test tube 2 to the side wall of the test tube 2, thereby preventing the incubation efficiency from being reduced. After the incubation oscillation is completed, in the process of extracting cells, in order to improve the purity of the cell fluid, the magnetic frame component 10 works to bring the plurality of magnets 102 close to the bottom of the test tube 2 to adsorb the magnetic microspheres in the test tube 2 to the side wall of the test tube 2. Such a configuration can ensure the purity of the cell fluid and prevent the magnetic microspheres in the test tube 2 from being adsorbed to the side wall of the test tube 2. interference; during the experimental cleaning process, in order to completely suck out the waste liquid in the test tube and prevent the magnetic microspheres in the test tube 2 from being sucked away, at this time, the magnetic frame assembly 10 does not work, so that the plurality of magnets 102 are stationary at the bottom of the test tube 2. At this time, the magnetic microspheres in the test tube 2 are adsorbed on the side wall of the test tube 2, and the cleaning assembly works to make the 40 cleaning needles 42 descend into the interior of the 40 test tubes 2 to suck away the residual waste liquid inside the test tube 2. At this time, the magnetic microspheres are still located in the test tube 2, which is convenient for use in the next incubation process, and before the next incubation oscillation, the magnetic frame assembly 10 works to move the plurality of magnets 102 away from the bottom of the test tube 2, which is convenient for the next incubation oscillation.

[0062] Preferably, if Figure 6As shown, the horizontal moving structure 6 is provided with a third drive 61, two first synchronous wheels 62 and a first synchronous belt 63. The third drive 61 is fixedly installed in the shell 1. The two first synchronous wheels 62 are respectively rotatably connected to the two ends of the shell 1. The two first synchronous wheels 62 are connected through the first synchronous belt 63. The output end of the third drive 61 is transmission-connected to any one of the two first synchronous wheels 62. The vertical moving structure 5 is arranged on the first synchronous belt 63. The third drive 61 can drive the first synchronous wheel 62 to rotate, thereby driving the first synchronous belt 63 to move, and then realizing the vertical moving structure 5 to move in the horizontal direction.

[0063] Preferably, the sample incubation and cleaning device for the lysis and eluent further includes a second guide rail 11, which is installed in the housing 1, the vertical moving structure 5 is slidably connected to the second guide rail 11, and the vertical moving structure 5 can move along the second guide rail 11 driven by the first synchronous belt 63. Specifically, the sample incubation and cleaning device for the lysis and eluent is provided with a second guide rail 11, which is horizontally installed in the housing 1, and the vertical moving structure 5 is slidably connected to the second guide rail 11. With such a configuration, the vertical moving structure 5 can move along the second guide rail 11 driven by the first synchronous belt 63, thereby preventing the vertical moving structure 5 from deflecting.

[0064] Preferably, the sample incubation and cleaning device for the lysis and elution instrument further includes two first stoppers, which are respectively installed at the two ends of the second guide rail 11. Specifically, the two first stoppers are respectively arranged at the two ends of the second guide rail 11, and the vertical moving structure 5 can respectively abut against the two first stoppers, so as to prevent the vertical moving structure 5 from being separated from the second guide rail 11.

[0065] Optionally, the first limiting member is a screw, and the first limiting member is threadedly connected to the second guide rail 11 .

[0066] Preferably, the vertical moving structure 5 includes a third guide rail 51, a fourth drive 52, two second synchronous wheels 53 and a second synchronous belt 54. The third guide rail 51 is fixedly connected to the first synchronous belt 63, and the third guide rail 51 is slidably connected to the second guide rail 11. The fourth drive 52 is installed on the third guide rail 51. The two second synchronous wheels 53 are rotatably installed on both ends of the third guide rail 51. The second synchronous belt 54 connects the two second synchronous wheels 53. The output end of the fourth drive 52 is connected to any one of the two second synchronous wheels 53. The cleaning head 4 is slidably connected to the third guide rail 51. Specifically, the vertical moving structure 5 is provided with a third guide rail 51, a fourth drive 52, two second synchronous wheels 53 and a second synchronous belt 54. The third guide rail 51 is vertically connected to the first synchronous belt 63, and the third guide rail 51 is slidably connected to the second guide rail 11, the mounting member 41 is slidably connected to the third guide rail 51, the fourth drive 52 is fixedly installed on the third guide rail 51, the two second synchronous wheels 53 are respectively rotatably connected to the two ends of the third guide rail 51, the two second synchronous wheels 53 are connected through the second synchronous belt 54, and the output end of the fourth drive 52 is connected to any one of the two second synchronous wheels 53. With such a configuration, the fourth drive 52 can drive the second synchronous wheel 53 to rotate, thereby driving the second synchronous belt 54 to move, and then driving the mounting member 41 to move along the third guide rail 51, so as to realize the movement of the cleaning needle 42 in the vertical direction and prevent the cleaning needle 42 from offset.

[0067] Preferably, the sample incubation and cleaning device for the lysis and elution instrument further includes two second stoppers, which are respectively mounted at both ends of the third guide rail 51. Specifically, the two second stoppers are respectively arranged at both ends of the third guide rail 51, and the mounting member 41 can respectively abut against the two second stoppers, thereby preventing the mounting member 41 from being separated from the third guide rail 51.

[0068] Optionally, the second limiting member is a screw, and the second limiting member is threadedly connected to the third guide rail 51 .

[0069] Preferably, if Figure 1 and Figure 6 As shown, the sample incubation and cleaning device for the lysis and elution instrument also includes a light-shielding cover 13, which is slidably arranged on the top of the incubation chamber 3. A through hole 31 is arranged on the top of the incubation chamber 3. The light-shielding cover 13 can close the through hole 31 to prevent light from entering the incubation chamber 3 and avoid affecting the incubation of the sample.

[0070] Preferably, the sample incubation and cleaning device for the lysis and elution instrument also includes a fourth guide rail, a fifth drive 15, two sixth synchronous wheels 16 and a fourth synchronous belt 17. The fourth guide rail is horizontally installed in the incubation chamber 3. The light-shielding cover 13 is slidably connected to the fourth guide rail. The two sixth synchronous wheels 16 are respectively rotatably connected to the two ends of the fourth guide rail. The two sixth synchronous wheels 16 are connected through the fourth synchronous belt 17. The fifth drive 15 is transmission-connected to any one of the two sixth synchronous wheels 16. With such an arrangement, the fifth drive 15 can drive the sixth synchronous wheel 16 to rotate, thereby driving the fourth synchronous belt 17 to move, and then driving the light-shielding cover 13 to move along the fourth guide rail, thereby realizing the opening and closing of the light-shielding cover 13 and preventing the light-shielding cover 13 from shifting.

[0071] Preferably, the sample incubation and cleaning device for the lysis and elution instrument further includes two third stoppers, which are respectively installed at the two ends of the fourth guide rail. Specifically, the two third stoppers are respectively arranged at the two ends of the fourth guide rail, and the light shielding cover 13 can respectively abut against the two third stoppers, thereby preventing the light shielding cover 13 from separating from the fourth guide rail.

[0072] Optionally, the third limiting member is a screw, and the third limiting member is threadedly connected to the fourth guide rail.

[0073] Preferably, the sample incubation and cleaning device for the lysis and elution instrument also includes a test tube rack 14, which is used to place test tubes 2. An opening 32 is provided on one side of the incubation chamber 3. The test tube rack 14 can enter the incubation chamber 3 through the opening 32 and be placed on the oscillation seat 91, which is convenient for batch placement of test tubes 2.

[0074] The utility model provides a sample incubation and cleaning device for a lysis and elution instrument, which reduces the equipment and objects required for the experiment, performs integrated processing, and standardizes the experimental operation through equipment control. The use process of the device is as follows:

[0075] 1. Place the test tube 2 containing the sample on the test tube carrier 14, and put the test tube carrier 14 into the incubation chamber 3 through the opening 32 and place it on the oscillation seat 91, start the first drive 93, so that the eccentric wheel 92 drives the oscillation seat 91 to move, so as to realize the vortex mixing operation of the liquid in the test tube 2;

[0076] 2. Start the heating assembly 8 to perform a heating operation, so that the temperature reaches 37°C ± 1°C accurately, and start the fifth drive 15 at the same time, close the light shielding cover 13, so that the incubation chamber 3 is in a suitable environment with constant temperature and light avoidance;

[0077] 3. After the incubation phase is completed, the second drive 104 is started to raise the magnet 102 to the bottom of the test tube 2 and keep it in a static state for 1-2 minutes to ensure that all magnetically encoded microspheres are completely adsorbed on the side wall of the test tube 2;

[0078] 4. In the experimental cleaning stage, the fifth drive 15 is reversely started, the light shielding cover 13 is opened, and the third drive 61 is started to move the cleaning needle 42 to the top of the test tube 2; the fourth drive 52 is started to make the 40 cleaning needles 42 descend into the test tube 2; the negative pressure pump is started to suck away the residual waste liquid in the test tube 2. During this process, the magnet 102 remains stationary and does not move in any way to prevent the magnetically encoded microspheres from being sucked away;

[0079] 5. After the waste liquid is completely absorbed and discharged, the fourth drive 52 is started in reverse to withdraw the cleaning needle 42 from the inside of the test tube 2; the third drive 61 is started in reverse to move the cleaning needle 42 to the top of the cleaning structure 7; the fourth drive 52 is started to make the 40 cleaning needles 42 descend into the inside of the 40 cleaning chambers, and the cleaning liquid is injected to perform the cleaning operation of the cleaning needles 42.

[0080] 6. Start the second drive 104 in reverse direction to lower the magnet 102;

[0081] 7. If incubation and cleaning are still required, repeat the above steps.

[0082] In addition, the above are only preferred embodiments of the present invention and the technical principles used. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and that various obvious changes, readjustments and substitutions can be made by those skilled in the art without departing from the scope of protection of the present invention. Therefore, although the present invention is described in more detail through the above embodiments, the present invention is not limited to the above embodiments, and may include more other equivalent embodiments without departing from the concept of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. A sample incubation and cleaning device for a lysis and elution instrument, characterized in that: include: Housing (1); A test tube (2), the test tube (2) being used to contain a sample; an incubation chamber (3), wherein the incubation chamber (3) is arranged in the housing (1), and the test tube (2) is arranged in the incubation chamber (3); A cleaning component, the cleaning component comprising a cleaning head (4), a vertical moving structure (5), a horizontal moving structure (6) and a cleaning structure (7), wherein the cleaning structure (7) is installed in the shell (1), one end of the vertical moving structure (5) is connected to the cleaning head (4), the other end of the vertical moving structure (5) passes through the shell (1) and is connected to the horizontal moving structure (6), the cleaning head (4) is used to clean the test tube (2), the cleaning head (4) can pass through the shell (1) and be inserted into the cleaning structure (7), and the cleaning structure (7) is used to clean the cleaning head (4).

2. The sample incubation and cleaning device for a lysis and elution instrument according to claim 1, characterized in that: The cleaning head (4) comprises a mounting member (41) and a plurality of cleaning needles (42); the mounting member (41) is mounted on a vertical moving structure (5); a plurality of cleaning needles (42) are evenly mounted on the mounting member (41); and the cleaning structure (7) is capable of cleaning the cleaning needles (42).

3. The sample incubation and cleaning device for a lysis and elution instrument according to claim 1, characterized in that: The sample incubation and cleaning device for the lysis and elution instrument further comprises a heating component (8), and the heating component (8) is installed in the incubation chamber (3).

4. The sample incubation and cleaning device for a lysis and elution instrument according to claim 1, characterized in that: The sample incubation and cleaning device for the lysis and elution instrument also includes an oscillation component (9), which is installed in the incubation chamber (3). The oscillation component (9) includes an oscillation seat (91), an eccentric wheel (92) and a first drive (93). The oscillation seat (91) is connected to the eccentric part of the eccentric wheel (92), and the eccentric wheel (92) is connected to the output end of the first drive (93). The test tube (2) is arranged on the oscillation seat (91).

5. The sample incubation and cleaning device for a lysis and elution instrument according to claim 4, characterized in that: The sample incubation and cleaning device for the lysis and elution instrument also includes a magnetic frame assembly (10), which is installed in the incubation chamber (3) and is located below the oscillation seat (91). The magnetic frame assembly (10) includes a fixed frame (101), a plurality of magnets (102), a first guide rail (103) and a second drive (104). The first guide rail (103) is arranged in the incubation chamber (3), the fixed frame (101) is slidably connected to the first guide rail (103), the plurality of magnets (102) are fixedly installed on the fixed frame (101), and the output end of the second drive (104) is connected to the fixed frame (101).

6. The sample incubation and cleaning device for a lysis and elution instrument according to any one of claims 1 to 5, characterized in that: The horizontal moving structure (6) comprises a third drive (61), two first synchronous wheels (62) and a first synchronous belt (63); the third drive (61) is installed in the housing (1); the two first synchronous wheels (62) are rotatably installed at two ends of the housing (1); the first synchronous belt (63) is simultaneously sleeved with the two first synchronous wheels (62); the output end of the third drive (61) is connected to any one of the two first synchronous wheels (62); and the vertical moving structure (5) is installed on the first synchronous belt (63).

7. The sample incubation and cleaning device for a lysis and elution instrument according to claim 6, characterized in that: The sample incubation and cleaning device for the lysis and elution instrument also includes a second guide rail (11), which is installed in the shell (1), and the vertical moving structure (5) is slidably connected to the second guide rail (11), and the vertical moving structure (5) can move along the extension direction of the second guide rail (11) under the drive of the first synchronous belt (63).

8. The sample incubation and cleaning device for a lysis and elution instrument according to claim 7, characterized in that: The sample incubation and cleaning device for the lysis and elution instrument further comprises two first stoppers, which are respectively mounted at two ends of the second guide rail (11), and the vertical moving structure (5) can respectively abut against the two first stoppers.

9. The sample incubation and cleaning device for a lysis and elution instrument according to claim 7, characterized in that: The vertical moving structure (5) comprises a third guide rail (51), a fourth drive (52), two second synchronous wheels (53) and a second synchronous belt (54); the third guide rail (51) is fixedly connected to the first synchronous belt (63), and the third guide rail (51) is slidably connected to the second guide rail (11); the fourth drive (52) is mounted on the third guide rail (51); the two second synchronous wheels (53) are rotatably mounted on the two ends of the third guide rail (51); the second synchronous belt (54) is simultaneously sleeved with the two second synchronous wheels (53); the output end of the fourth drive (52) is connected to any one of the two second synchronous wheels (53); and the cleaning head (4) is slidably connected to the third guide rail (51).

10. The sample incubation and cleaning device for a lysis and elution instrument according to claim 9, characterized in that: The sample incubation and cleaning device for the lysis and elution instrument further comprises two second stoppers, the two second stoppers being respectively mounted at the two ends of the third guide rail (51), and the cleaning head (4) can respectively abut against the two second stoppers.

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