Pretreatment device for flow cytometry detection

Abstract

The utility model provides a pretreatment device for flow cytometry, which relates to the technical field of biological medicine and is characterized in that an L-shaped sliding shaft is slidably mounted at an opening part at the top end of a filter tube through pushing of a spring, a vertical sliding shaft is connected onto the L-shaped sliding shaft, and the vertical sliding shaft is matched with the central part of the filter tube in an inserting manner; the middle part of the vertical sliding shaft is fixedly sleeved with a filter screen assembly, and a plug is welded to the bottom end of the vertical sliding shaft; a vertically-arranged pushing shaft is welded to the outer ring position of the tray, when the filter pipe slides downwards to be combined with the test tube in an inserted mode, the L-shaped sliding shaft abuts against and makes contact with the pushing shaft, and when the pushing shaft slides upwards to be separated from the test tube, the plug slides downwards to block an opening in the bottom end of the filter pipe. Through the power transmission of the L-shaped sliding shaft and the pushing shaft, the plug can be automatically loosened by indirectly utilizing the up-and-down sliding of the filter tube and the pulling, inserting, dismounting and mounting driving force of the test tube, so that the trouble of additionally manually loosening and locking the switch plug before and after the detection operation is saved, and the operation and the use are simple and convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedicine, in particular to a flow cell detection pre-processing device. Background Art

[0002] Flow cytometry is a method for quantitative cell analysis. It can perform rapid, high-precision and multi-parameter detection of single cells. It is one of the most advanced technologies currently available. Before quantitative cell analysis, cells in a fluid state must be pre-filtered and purified, which requires the use of a special pretreatment device.

[0003] In the existing processing device, the part for performing flow filtration on the cells is generally open at the bottom after the test is completed and the test tube or other container containing the filtered cells is extracted and separated, and lacks shielding and blocking, so that the residual cell fluid therein may drip and stick to the test tube container to be extracted and disassembled and the fingers for extracting the test tube container, which requires the trouble of subsequent cleaning;

[0004] In addition, although some test tube containers are provided with openable and closable components on the bottom opening, these components need to be manually opened and closed before and after the test is completed, which is troublesome and inconvenient to operate. Utility Model Content

[0005] In view of this, the utility model provides a flow cytometry pre-processing device to solve the problem that although some test tube containers are provided with openable and closable components on the bottom opening, these components need to be manually opened and closed before and after the detection is completed, which is troublesome and inconvenient to operate.

[0006] The technical solution proposed by the utility model is: a flow cytometry pre-processing device, specifically comprising a base ring, a test tube and a filter tube, the top of the base ring is welded with a hexagonal vertical support shaft, and the filter tube is lifted and slidably installed on the hexagonal vertical support shaft;

[0007] The hexagonal vertical support shaft is welded with a limiting collar and a tray at intervals up and down, the test tube is plugged into the limiting collar and supported on the tray, and the part of the hexagonal vertical support shaft located above the limiting collar is slidably installed with a sliding mounting piece through a spring push; the sliding mounting piece is composed of a head end pipe ring and a tail end shaft ring welded together, and the filter tube is plugged into the pipe ring and positioned inside the pipe ring;

[0008] The top opening of the filter tube is slidably mounted with an L-shaped sliding shaft through a spring push, and the L-shaped sliding shaft is connected to a vertical sliding shaft, which is plugged and matched with the central part of the filter tube; the middle part of the vertical sliding shaft is fixedly sleeved with a filter assembly, and a plug is welded at the bottom;

[0009] A vertically arranged push shaft is welded to the outer ring of the tray. When the filter tube slides downward and is plugged into the test tube, the L-shaped sliding shaft abuts against the push shaft. When the push shaft slides upward and separates from the test tube, the plug slides down and blocks the bottom opening of the filter tube.

[0010] Furthermore,

[0011] A U-shaped frame is welded on the pipe ring, and the U-shaped frame is used for fingers to hook and pull force.

[0012] Furthermore,

[0013] A limiting disk is welded to the top end of the hexagonal vertical support shaft.

[0014] Furthermore, a circle of railings is welded around the outer circumference of the tray, and a receiving groove is placed on the top of the tray. The receiving groove is limited between the circle of railings, and the bottom end of the test tube is in contact with the receiving groove.

[0015] Furthermore,

[0016] An inclined connecting plate is welded between the positioning sleeve and the vertical sliding shaft, and both ends of the inclined connecting plate are bent structures;

[0017] A conical funnel is arranged at the top of the filter tube, the main body of the inclined connecting plate is kept parallel to the circumferential side wall of the conical funnel, and a positioning sleeve is welded on the outer ring of the conical funnel, and the L-shaped sliding shaft is pushed by a spring to slide through and fit with the positioning sleeve.

[0018] Furthermore,

[0019] The filter assembly is composed of an outer retaining ring, a three-pronged frame fixed to the inner side of the retaining ring, and a filter covering the three-pronged frame and the retaining ring, wherein the outer ring of the retaining ring is sleeved with a sealing ring.

[0020] Furthermore,

[0021] The conical funnel and the filter tube are integrally formed.

[0022] Furthermore,

[0023] The conical funnel is detachably connected to the top end of the filter tube.

[0024] The utility model provides a flow cytometry pre-processing device, which has the following beneficial effects:

[0025] When the utility model is in use, the plug is used to close the bottom opening of the filter tube when the filter tube is lifted and drawn away from the test tube, thereby sealing the fluid cells inside the filter tube. Compared with the prior art in which the bottom opening of the filter component is open and lacks a plugging component, the utility model can prevent the residual liquid in the filter component from dripping and sticking to the test tube container and the fingers used to extract the test tube when the test tube container is extracted and disassembled, thereby saving the trouble of subsequent cleaning.

[0026] In addition, through the power transmission of the L-shaped sliding shaft and the pushing shaft, the plug can indirectly use the driving force of the up and down sliding of the filter tube and the insertion and disassembly of the test tube to loosen and tighten itself. This saves the trouble of manually loosening and tightening the plug before and after the detection operation, and is easy to operate and use.

[0027] In addition, when the conical funnel is disassembled and separated from the filter tube, the filter assembly can be pulled out and exposed for convenient cleaning.

[0028] In addition, the inclined connecting plate remains parallel to the circumferential side wall of the conical funnel, which makes the inclined connecting plate adapt to the shape of the conical funnel and can fit into or approach the side wall of the conical funnel, leaving most of the space inside the conical funnel free, reducing the occupancy of the internal space of the conical funnel and avoiding obstruction and interference in the pouring operation of the fluid cells into the conical funnel. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solution of the embodiment of the utility model, the drawings of the embodiment will be briefly introduced below.

[0030] The drawings described below only relate to some embodiments of the present invention, but are not intended to limit the present invention.

[0031] In the attached picture:

[0032] Figure 1 The overall front side structure schematic diagram of the first embodiment of the utility model is shown;

[0033] Figure 2 The overall rear side structure schematic diagram of the first embodiment of the utility model is shown;

[0034] Figure 3 A schematic diagram of the inner side structure of a half-section of a filter tube in Embodiment 1 of the utility model is shown;

[0035] Figure 4 A schematic diagram showing a disassembled state of the sliding mounting member of the first embodiment of the utility model is shown;

[0036] Figure 5 A schematic diagram of the inner side structure of a half-section filter tube in Embodiment 2 of the present utility model is shown;

[0037] Figure 6A schematic diagram showing the plug-in assembly state of the filter tube and the test tube in the second embodiment of the utility model is shown;

[0038] Reference numerals list

[0039] 1. Base ring; 101. Hexagonal vertical support shaft; 102. Tray; 103. Limit plate; 104. Push shaft; 105. Limit collar;

[0040] 2. Receive trough;

[0041] 3. Test tube;

[0042] 4. Sliding mounting member; 401. U-shaped frame;

[0043] 5. Filter tube; 501. Positioning sleeve; 502. L-shaped sliding shaft; 503. Inclined connecting plate; 504. Vertical sliding shaft; 505. Filter assembly; 506. Plug. DETAILED DESCRIPTION

[0044] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the described embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0045] Example 1, please refer to Figures 1 to 6 :

[0046] The utility model proposes a flow cytometry pre-processing device, comprising a base ring 1, a test tube 3 and a filter tube 5. A hexagonal vertical support shaft 101 is welded on the top of the base ring 1, and the filter tube 5 is lifted and slidably installed on the hexagonal vertical support shaft 101.

[0047] Among them, the limiting collar 105 and the tray 102 are welded on the hexagonal vertical support shaft 101 at intervals up and down, the test tube 3 is plugged and matched with the limiting collar 105 and supported on the tray 102, and the part of the hexagonal vertical support shaft 101 located above the limiting collar 105 is slidably installed with a sliding mounting member 4 through a spring push; the sliding mounting member 4 is composed of a head end tube ring and a tail end shaft ring welded together, and the filter tube 5 is plugged and matched with the tube ring and positioned inside the tube ring. When in use, the filter tube 5 is plugged and matched with the test tube 3, and the fluid cells are poured into the filter tube 5. When the fluid cells pass through the filter mesh assembly 505, they are filtered and purified, and the impurities therein are trapped at the top of the filter mesh assembly 505. The filtered cell liquid passes through the filter mesh assembly 505 and is discharged into the filter tube 5 for storage, waiting for subsequent detection;

[0048] The top opening of the filter tube 5 is slidably installed with an L-shaped sliding shaft 502 through a spring push, and a vertical sliding shaft 504 is connected to the L-shaped sliding shaft 502, and the vertical sliding shaft 504 is plugged and matched with the central part of the filter tube 5; the middle part of the vertical sliding shaft 504 is fixedly sleeved with a filter assembly 505, and a plug 506 is welded at the bottom end. The plug 506 is used to close the bottom opening of the filter tube 5 when the filter tube 5 is lifted and pulled away from the test tube 3, and to block the fluid cells inside the filter tube 5. Compared with the prior art in which the bottom opening of the filter component is open and lacks a plugging component, it can prevent the residual liquid in the filter component from dripping and sticking to the test tube container and the fingers used to extract the test tube when extracting and disassembling the test tube container, thereby saving the trouble of subsequent cleaning;

[0049] A vertically arranged pushing shaft 104 is welded to the outer ring position of the tray 102. When the filter tube 5 slides downward and is plugged into the test tube 3, the L-shaped sliding shaft 502 and the pushing shaft 104 abut against each other, and the vertical sliding shaft 504 can be pushed upward and drive the control plug 506 to lift and open. When the detection is completed, the filter tube 5 slides upward and is separated from the test tube 3. When the L-shaped sliding shaft 502 is disengaged from the pushing shaft 104, the L-shaped sliding shaft 502 loses its upward pushing holding force and can be pushed down and reset by the spring rebound thereon and drive 506 to abut against and block the bottom end opening of the filter tube 5. Then, through the power transmission between the L-shaped sliding shaft 502 and the pushing shaft 104, the plug 506 can indirectly use the driving force of the up and down sliding of the filter tube 5 and the insertion and disassembly of the test tube 3 to loosen and tighten itself. This saves the trouble of manually loosening and tightening the plug 506 before and after the detection operation, and is easy to operate and use.

[0050] Preferably,

[0051] A U-shaped frame 401 is welded on the pipe ring, and the U-shaped frame 401 is used for fingers to hook and pull force.

[0052] Preferably,

[0053] A limiting plate 103 is welded to the top of the hexagonal vertical support shaft 101 .

[0054] Preferably, a circle of railings is welded around the outer circumference of the tray 102, and a receiving groove 2 is placed on the top of the tray 102. The receiving groove 2 is limited between the circle of railings, and the bottom end of the test tube 3 is in contact with the receiving groove 2.

[0055] Preferably,

[0056] An inclined connecting plate 503 is welded between the positioning sleeve 501 and the vertical sliding shaft 504, and both ends of the inclined connecting plate 503 are bent structures;

[0057] A conical funnel is arranged at the top of the filter tube 5, and the inclined connecting plate 503 is kept parallel to the circumferential side wall of the conical funnel, so that the inclined connecting plate 503 is adapted to the shape of the conical funnel and can fit in or be close to the side wall of the conical funnel, leaving most of the space inside the conical funnel free, thereby reducing the occupation of the internal space of the conical funnel and avoiding obstruction and interference in the pouring operation of the fluid cells into the conical funnel. A positioning sleeve 501 is welded on the outer ring of the conical funnel, and the L-shaped sliding shaft 502 is slidably fitted with the positioning sleeve 501 through a spring push.

[0058] Preferably,

[0059] The filter assembly 505 is composed of an outer retaining ring, a three-pronged frame fixed to the inner side of the retaining ring, and a filter covering the three-pronged frame and the retaining ring, wherein the outer ring of the retaining ring is provided with a sealing ring.

[0060] Preferably,

[0061] The conical funnel and the filter tube 5 are integrally formed.

[0062] On the basis of the first embodiment, the second embodiment, as Figure 5 to Figure 6 As shown:

[0063] The conical funnel is detachably connected to the top of the filter tube 5. When the conical funnel is detached from the filter tube 5, the filter assembly 505 can be pulled out and exposed for cleaning. Compared with the first embodiment in which the filter assembly 505 is integrated and installed inside the filter tube 5, it is more convenient to clean the filter assembly 505.

[0064] Working principle: During the test, the filter tube 5 is plugged into the test tube 3, and the fluid cells are poured into the filter tube 5. When the fluid cells pass through the filter mesh assembly 505, they are filtered and purified, and the impurities therein are trapped at the top of the filter mesh assembly 505. The filtered cell liquid passes through the filter mesh assembly 505 and is discharged into the filter tube 5 for storage, waiting for subsequent testing. After the test is completed, the filter tube 5 and the test tube 3 are lifted and separated, and the test tube 3 is extracted and disassembled;

[0065] The plug 506 is used to close the bottom opening of the filter tube 5 when the filter tube 5 is lifted and separated from the test tube 3, thereby blocking the fluid cells inside the filter tube 5, and preventing the residual liquid in the filter component from dripping and dirtying the test tube container and the fingers used to extract the test tube when the test tube container is extracted and disassembled;

[0066] When the filter tube 5 slides downward and is plugged into the test tube 3, the L-shaped sliding shaft 502 comes into contact with the pushing shaft 104, and the vertical sliding shaft 504 can be pushed upward to drive the control plug 506 to be lifted up and opened. When the detection is completed, the filter tube 5 slides upward and is separated from the test tube 3. When the L-shaped sliding shaft 502 is disengaged from the pushing shaft 104, the L-shaped sliding shaft 502 loses its upward pushing holding force and can be pushed down and reset by the spring rebound thereon and drive 506 to come into contact with the bottom opening of the filter tube 5.

[0067] In this article, there are a few points to note:

[0068] 1. The drawings of the embodiments of the present utility model only involve structures related to the embodiments of the present utility model, and other structures can refer to the usual design.

[0069] 2. In the absence of conflict, the embodiments of the present invention and the features therein may be combined with each other to obtain new embodiments.

[0070] The above are only specific implementations of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A flow cytometry pre-processing device, comprising: A base ring (1), a test tube (3), a filter tube (5) and a positioning sleeve (501); a hexagonal vertical support shaft (101) is welded to the top of the base ring (1); and the filter tube (5) is installed on the hexagonal vertical support shaft (101) in a lifting and sliding manner; The invention is characterized in that a limiting collar (105) and a tray (102) are welded on the hexagonal vertical support shaft (101) at intervals up and down, the test tube (3) is plugged into and matched with the limiting collar (105) and supported on the tray (102), and the part of the hexagonal vertical support shaft (101) located above the limiting collar (105) is slidably installed with a sliding mounting member (4) through a spring push; the sliding mounting member (4) is composed of a head end pipe ring and a tail end shaft ring welded together, and the filter tube (5) is plugged into and matched with the pipe ring and positioned inside the pipe ring; The top opening of the filter tube (5) is slidably mounted with an L-shaped sliding shaft (502) through a spring push, and the L-shaped sliding shaft (502) is connected to a vertical sliding shaft (504), and the vertical sliding shaft (504) is plugged and matched with the central part of the filter tube (5); the middle part of the vertical sliding shaft (504) is fixedly sleeved with a filter assembly (505), and a plug (506) is welded to the bottom end; A vertically arranged push shaft (104) is welded to the outer ring of the tray (102); when the filter tube (5) slides downward to be plugged into the test tube (3), the L-shaped slide shaft (502) and the push shaft (104) come into contact with each other; when the push shaft (104) slides upward to be separated from the test tube (3), the plug (506) slides down to block the bottom opening of the filter tube (5).

2. A flow cytometry pre-processing device according to claim 1, characterized in that: A U-shaped frame (401) is welded on the pipe ring, and the U-shaped frame (401) is used for fingers to hook and pull out force.

3. A flow cytometry pre-processing device according to claim 1, characterized in that: A limiting plate (103) is welded to the top end of the hexagonal vertical support shaft (101).

4. A flow cytometry pre-processing device according to claim 1, characterized in that: A circle of railings is welded around the outer circumference of the tray (102), and a receiving groove (2) is placed on the top of the tray (102). The receiving groove (2) is limited between the circle of railings, and the bottom end of the test tube (3) is in contact with the receiving groove (2).

5. A flow cytometry pre-processing device according to claim 1, characterized in that: An inclined connecting plate (503) is welded between the positioning sleeve (501) and the vertical sliding shaft (504), and both ends of the inclined connecting plate (503) are in a bent structure; A conical funnel is arranged at the top end of the filter tube (5), the main body of the inclined connecting plate (503) is kept parallel to the circumferential side wall of the conical funnel, and a positioning sleeve (501) is welded to the outer ring of the conical funnel, and the L-shaped sliding shaft (502) is pushed by a spring to slide through and fit with the positioning sleeve (501).

6. A flow cytometry pre-processing device according to claim 1, characterized in that: The filter assembly (505) is composed of an outer retaining ring, a three-pronged frame fixed to the inner side of the retaining ring, and a filter covering the three-pronged frame and the retaining ring, wherein the outer ring of the retaining ring is sleeved with a sealing ring.

7. A flow cytometry pre-processing device according to claim 5, characterized in that: The conical funnel and the filter tube (5) are integrally formed.

8. A flow cytometry pre-processing device according to claim 5, characterized in that: The conical funnel is detachably connected to the top end of the filter tube (5).

Images