High-compatibility portable oocyte operation needle storage box

Abstract

The utility model discloses a high-compatibility portable oocyte operation needle storage box which comprises a box body, a cover body is arranged above the box body, the box body and the cover body are both of a cuboid structure, the cover body and the box body are symmetrically arranged up and down, the box body is rotationally connected with one side of the cover body, and the box body is fixedly buckled with the other side of the cover body; the protective layer is arranged at the bottom of the inner side of the box body and is used for protecting an oocyte operation needle; an adhesive tape is arranged at the bottom of the inner side of the box body, and the protective layer is adhered and fixed with the box body through the adhesive tape; the number of the needle placing tables is two, and the two needle placing tables are respectively arranged at two ends of the top surface of the protective layer and are respectively used for fixing two oocyte operation needles with different lengths. According to the utility model, the double needle placing tables are arranged, so that two glass capillary needles with different lengths can be compatible at the same time, the needle replacing time is saved, the experiment time is shortened, the damage to oocytes due to long-term exposure in an in-vitro operation environment is reduced, and the experiment efficiency and the success rate are favorably improved.

Description

Technical Field

[0001] The utility model relates to the technical field of storage boxes, in particular to a highly compatible portable oocyte operating needle storage box. Background Art

[0002] In the ovaries of female animals, oocytes undergo a series of cell division and development processes, and eventually form mature eggs. When male sperm combines with female eggs, fertilized eggs are formed, genetic materials merge, and life begins. Therefore, oocytes are of great significance for the reproduction of offspring. In the field of developmental biology research, mice, rats, and pigs are often used as models to collect oocytes from the ovarian tissue of female experimental animals. According to experimental requirements, oocytes with specific morphology or characteristics are screened and transferred for subsequent research. Among them, the oocyte manipulation needle is an important tool for completing this experiment. The oocyte manipulation needle uses a glass capillary needle, and good storage of the glass capillary needle is a prerequisite for ensuring the smooth progress of the experiment.

[0003] A common storage method is to store glass capillary needles of the same specifications in a cylindrical "storage tank". The upper part of the "storage tank" is provided with a foam card slot for fixing the glass capillary needle. The glass capillary needle is placed vertically downward ("flat end" facing up, "fine tip" facing down) into the foam card slot. During the experimental operation, the experimenter will first unscrew the "storage tank", then use metal tweezers to carefully clamp the glass capillary needle in it, and then install the "flat end" of the glass capillary needle at the end of the "mouth straw" to form a complete operating device. Under a stereo microscope, use the "fine tip" of the glass capillary needle to select and transfer oocytes. After the experiment, the glass capillary needle is carefully placed back in the corresponding card position in the "storage tank" for the next experiment. In addition, during the experiment, when a new glass capillary needle is used, it is only necessary to take a new glass capillary needle from the "storage tank" and perform the above operation.

[0004] However, the existing "storage tank" has the following problems: 1. The "storage tank" is cylindrical. During the experiment, if the experimenter is a little careless, it will be knocked down. The fallen "storage tank" will roll on the ground, causing the glass capillary needle inside to fall off and be damaged. Therefore, this "storage tank" needs to be placed in a specific area away from the experimenter, which greatly reduces the convenience of taking and placing the glass capillary needle; 2. The glass capillary needles in the tank are placed vertically downward, and their center of gravity is biased downward. Since there is no shock-absorbing support installed near the "fine tip", during transportation, the "storage tank" sometimes lies horizontally. Slight bumps and jolts can cause the glass capillary needles near the bottom of the tank to tilt due to gravity and contact the inner wall of the tank, eventually resulting in the breakage of the needle tip; 3. The foam card slot of the "storage tank" has size limitations on the diameter of the glass capillary needle. After repeatedly taking and placing the glass capillary needle, irreversible deformation will occur in the foam card slot, and finally the loose foam card slot can only be discarded, which reduces its scope of application and service life; 4. When picking and transferring oocytes, the experimenter often uses two glass capillary needles of different lengths to successively perform operations such as cell stripping, classification (short glass capillary needle) and aspiration, transfer (long glass capillary needle). However, the "storage tank" can only accommodate glass capillary needles of one length and is difficult to be compatible with two glass capillary needles of different lengths at the same time. This forces the experimenter to open another "storage tank" for the other length, which not only increases the experimental time but also reduces the experimental efficiency. Eventually, due to the long-term exposure of oocytes to the external operation environment, the cell activity will decrease and the experiment will fail.

[0005] To this end, the present application proposes a highly compatible and portable oocyte operation needle storage box, providing a new technical solution to solve the above-mentioned technical problems. Summary of the Utility Model

[0006] In order to solve the above technical problems, the present utility model adopts the following technical solutions:

[0007] A highly compatible and portable oocyte operation needle storage box, which is applied to the storage of oocyte operation needles.

[0008] The highly compatible and portable oocyte operation needle storage box specifically includes:

[0009] A box body, a cover body is arranged above the box body. Both the box body and the cover body are in a cuboid structure. The cover body and the box body are symmetrically arranged up and down. One side of the box body and the cover body is rotatably connected, and the other side of the box body and the cover body is fastened and fixed.

[0010] A protective layer, which is arranged at the bottom inside the box body for protecting the oocyte operation needle; a sticky tape is arranged at the bottom inside the box body, and the protective layer is adhesively fixed to the box body through the sticky tape.

[0011] There are two needle placement platforms, which are respectively arranged at both ends of the top surface of the protective layer and are respectively used for fixing oocyte manipulation needles of two different lengths.

[0012] As a preferred embodiment of the highly compatible portable oocyte manipulation needle storage box provided by the present utility model, the number of the adhesive strips is two, and the two adhesive strips are both arranged in parallel along the length direction of the box body at the inner bottom of the box body.

[0013] As a preferred embodiment of the highly compatible portable oocyte manipulation needle storage box provided by the present utility model, the protective layer includes an EPE pearl cotton foam board, an adhesive layer, and kraft paper arranged in sequence from bottom to top. The foam board is adhesively fixed to the box body through the adhesive strip, and the foam board and the kraft paper are adhesively connected through the adhesive layer.

[0014] As a preferred embodiment of the highly compatible portable oocyte manipulation needle storage box provided by the present utility model, the needle placement platform includes double-sided tape one, double-sided tape two, and transparent tape arranged in sequence from bottom to top and adhesively bonded to each other. The double-sided tape one is adhesively fixed to the top surface of the protective layer, and the adhesive surface of the transparent tape faces upward. The transparent tape is used for fixing the oocyte manipulation needle.

[0015] As a preferred embodiment of the highly compatible portable oocyte manipulation needle storage box provided by the present utility model, a buckle seat is fixedly connected to one side of the box body, and a buckle frame is fixedly connected to one side of the cover body. The buckle seat and the buckle frame are snap-connected.

[0016] As a preferred embodiment of the highly compatible portable oocyte manipulation needle storage box provided by the present utility model, the side of the box body and the cover body away from the buckle seat and the buckle frame is rotatably connected through a hinge shaft.

[0017] As a preferred embodiment of the highly compatible portable oocyte manipulation needle storage box provided by the present utility model, the foam board is an EPE pearl cotton foam board.

[0018] As a preferred embodiment of the highly compatible portable oocyte manipulation needle storage box provided by the present utility model, the oocyte manipulation needle is a glass capillary needle.

[0019] As a preferred embodiment of the highly compatible portable oocyte manipulation needle storage box provided by the present utility model, the glass capillary needle includes a long glass capillary needle and a short glass capillary needle, and the long glass capillary needle and the short glass capillary needle are respectively horizontally placed on the two needle placement platforms.

[0020] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0021] 1. The box body and the cover body of the present utility model are both in a cuboid structure. The bottom surface of the box body is rectangular, and the large contact area is beneficial to its stability. In addition, the corners of this storage box are distinct, reducing the risk of its rolling and falling. Placed within the reach of the experimenter, it improves the convenience of operation.

[0022] 2. The present utility model is provided with a protective layer at the bottom inside the box body. The protective layer can serve as a shock-absorbing structure, playing a role in buffering and protection. When the storage box is bumped during transportation, it can effectively disperse and reduce the external force on the glass capillary needle, avoiding the breakage of the needle tip.

[0023] 3. By setting the needle placement platform, the present utility model enables the center of gravity of the glass capillary needle to be well distributed on it, avoiding the displacement and falling of the glass capillary needle. Moreover, the surface of the needle placement platform has adhesiveness, allowing for the repeated picking and placing of the glass capillary needle and also capable of storing glass capillary needles of different diameters, which improves the service life and utilization rate of the needle placement platform. In addition, the design of the double needle placement platform enables this storage box to be compatible with two different lengths of glass capillary needles (long glass capillary needles and short glass capillary needles) simultaneously, saving the needle replacement time, shortening the overall experimental time, reducing the damage to the oocytes caused by long-term exposure to the in vitro operation environment, and contributing to improving the experimental efficiency and success rate. Description of the Drawings

[0024] Figure 1 is the overall structural schematic diagram of the highly compatible portable oocyte operation needle storage box provided by the present utility model;

[0025] Figure 2 is the rear view of the overall structure of the highly compatible portable oocyte operation needle storage box provided by the present utility model;

[0026] Figure 3 is the internal structural schematic diagram of the box body of the highly compatible portable oocyte operation needle storage box provided by the present utility model;

[0027] Figure 4 is the internal decomposition schematic diagram of the box body of the highly compatible portable oocyte operation needle storage box provided by the present utility model;

[0028] Figure 5 is the structural schematic diagram of the adhesive tape of the highly compatible portable oocyte operation needle storage box provided by the present utility model;

[0029] Figure 6 is the structural schematic diagram of the protective layer of the highly compatible portable oocyte operation needle storage box provided by the present utility model;

[0030] Figure 7 is the structural schematic diagram of the needle placement platform of the highly compatible portable oocyte operation needle storage box provided by the present utility model;

[0031] Figure 8 The structural schematic diagram of the glass capillary needle of the highly compatible portable oocyte operation needle storage box provided by the present utility model.

[0032] The markings in the figure are explained as follows:

[0033] 1. Box body; 2. Cover body; 3. Buckle base; 4. Buckle frame; 5. Hinge shaft; 6. Protective layer; 7. Needle placement table; 8. Adhesive tape; 9. Foam board; 10. Adhesive layer; 11. Kraft paper; 12. Double-sided tape one; 13. Double-sided tape two; 14. Transparent tape; 15. Glass capillary needle; 151. Long glass capillary needle; 152. Short glass capillary needle. Specific embodiments

[0034] The following will further describe the present utility model in detail with reference to the accompanying drawings.

[0035] The present utility model and its embodiments are described below. Such description is not restrictive, and the actual embodiments are not limited thereto. Generally speaking, if those of ordinary skill in the art are inspired by it and without departing from the creative purpose of the present utility model, without creative design, the structural modes and embodiments similar to the technical solution should all fall within the protection scope of the present utility model.

[0036] Reference Figure 1 - Figure 7 , the present utility model provides a highly compatible portable oocyte operation needle storage box, including:

[0037] A box body 1, a cover body 2 is arranged above the box body 1. Both the box body 1 and the cover body 2 are in a cuboid structure. The cover body 2 and the box body 1 are symmetrically arranged up and down. One side of the box body 1 and the cover body 2 is rotatably connected, and the other side of the box body 1 and the cover body 2 is fastened and fixed. Specifically, a buckle base 3 is fixedly connected to one side of the box body 1, a buckle frame 4 is fixedly connected to one side of the cover body 2, and the buckle base 3 and the buckle frame 4 are buckled and connected. The side of the box body 1 and the cover body 2 away from the buckle base 3 and the buckle frame 4 is rotatably connected through a hinge shaft 5. By arranging the buckle base 3, the buckle frame 4 and the hinge shaft 5 on both sides of the storage box, the integration and stability of the storage box can be improved.

[0038] A protective layer 6 is provided at the inner bottom of the box body 1 for protecting the oocyte manipulation needle. A sticky tape 8 is provided at the inner bottom of the box body 1, and the protective layer 6 is adhesively fixed to the box body 1 through the sticky tape 8. Among them, in order to ensure the firm fixation of the protective layer 6, the number of the sticky tapes 8 is two, and the two sticky tapes 8 are both arranged in parallel along the length direction of the box body 1 at the inner bottom of the box body 1. Specifically, the protective layer 6 includes an EPE pearl cotton foam board 9, an adhesive layer 10 and kraft paper 11 which are arranged in sequence from bottom to top. The foam board 9 is adhesively fixed to the box body 1 through the sticky tape 8, and the foam board 9 and the kraft paper 11 are adhesively connected through the adhesive layer 10. In this embodiment, the foam board 9 is an EPE pearl cotton foam board, which is a foam board made of polyethylene material. By providing the protective layer 6, the oocyte manipulation needle can be protected.

[0039] There are two needle placement platforms 7, and the two needle placement platforms 7 are respectively arranged at both ends of the top surface of the protective layer 6 and are respectively used for fixing two oocyte manipulation needles with different lengths. Specifically, the needle placement platform 7 includes a double-sided tape 12, a double-sided tape 13 and a transparent tape 14 which are arranged in sequence from bottom to top and are adhesively bonded to each other. The double-sided tape 12 is adhesively fixed to the top surface of the protective layer 6, and the adhesive surface of the transparent tape 14 faces upward. The transparent tape 14 is used for fixing the oocyte manipulation needle. In this embodiment, the oocyte manipulation needle is a glass capillary needle 15, and the glass capillary needle 15 includes a long glass capillary needle 151 and a short glass capillary needle 152. The long glass capillary needle 151 and the short glass capillary needle 152 are respectively horizontally placed on the two needle placement platforms 7 in an orderly arrangement, and both the long glass capillary needle 151 and the short glass capillary needle 152 are placed with the "flat end" facing outward and the "fine tip" facing inward. Through the stickiness of the surface of the needle placement platform 7, the long glass capillary needle 151 and the short glass capillary needle 152 can be stuck for fixation and storage.

[0040] The box body 1 and the cover body 2 of the present utility model are both in a cuboid structure. The bottom surface of the box body 1 is rectangular, and the larger contact area is beneficial to its stability. In addition, the corners of this storage box are distinct, reducing the risk of it rolling over and falling. Placed within the reach of the experimenter, it improves the convenience of operation. The present utility model is provided with a protective layer 6 at the inner bottom of the box body 1. The protective layer 6 can serve as a shock-absorbing structure, playing a role in buffering and protection. When the storage box is bumped during transportation, it can effectively disperse and reduce the external force on the glass capillary needle 15, avoiding the breakage of the needle tip. The present utility model makes the center of gravity of the glass capillary needle 15 be well distributed on it by setting the needle placement platform 7, avoiding the displacement and falling of the glass capillary needle 15; and the surface of the needle placement platform 7 has adhesiveness, enabling the glass capillary needle 15 to be repeatedly taken and placed, and can also store glass capillary needles 15 with different diameters, which improves the service life and utilization rate of the needle placement platform 7. In addition, the design of the double needle placement platform 7 enables this storage box to be compatible with two different lengths of glass capillary needles 15 (long glass capillary needle 151, short glass capillary needle 152) at the same time, saving the needle replacement time, shortening the overall experimental time, reducing the damage to the oocytes caused by long-term exposure to the in vitro operation environment, and helping to improve the experimental efficiency and success rate.

[0041] During assembly:

[0042] 1. First, neatly stick the adhesive tape 8 on the bottom of the box body 1;

[0043] 2. Adhere and fix the foam board 9 with a suitable size on the bottom of the box body 1;

[0044] 3. Then use the adhesive layer 10 to neatly stick the kraft paper 11 with a suitable size on the foam board 9 and roll it back and forth with a roller to compact it;

[0045] 4. Then stick the double-sided tape 12 and the double-sided tape 13 at one end of the kraft paper 11, so that the lengths of the double-sided tape 12 and the double-sided tape 13 are the same as the width of the kraft paper 11. Finally, lay the transparent tape 14 with the same width as the double-sided tape 13 on the double-sided tape 13 with the smooth surface facing up, so that the adhesive surface of the transparent tape 14 is upward and keep this surface neat without wrinkles and bubbles. Subsequently, repeat the above steps at the other end of the kraft paper 11 to install another needle placement platform 7;

[0046] 5. Finally, horizontally place the long glass capillary needle 151 and the short glass capillary needle 152 on the adhesive surfaces of the transparent tapes 14 at both ends respectively and make them arranged neatly and orderly, and then cover the cover body 2 to complete the storage work.

[0047] When in use;

[0048] When performing oocyte operations, first open the cover 2, use forceps to clamp the front end of the short glass capillary needle 152, then install the short glass capillary needle 152 at the tail of the "mouth pipette" and start the work of oocyte dissection and classification under a stereomicroscope. After the operation is completed, place the short glass capillary needle 152 back to its original position in the box body 1. Immediately afterwards, use the same method to clamp the long glass capillary needle 151, install it in the "mouth pipette" to perform the operation of oocyte aspiration and transfer. After all the experiments are completed, place the long glass capillary needle 151 back to its original position in the box body 1.

[0049] Oocytes are a type of suspension cell. In oocyte operation experiments, after the cells are fixed, the short glass capillary needle 152 is first used to carefully remove the granulosa cell layer to fully expose the oocyte sample. Subsequently, the oocytes are classified according to their size or morphology according to the experimental purpose. Immediately afterwards, the oocytes are aspirated and transferred for subsequent culture. The aspiration operation often inhales a large amount of operating fluid. Therefore, the long glass capillary needle 151 with a larger volume is commonly used to perform this step. Therefore, the compatible design of the long glass capillary needle 151 and the short glass capillary needle 152 saves the needle-changing time, shortens the overall experimental time, reduces the damage to the oocytes caused by long-term exposure to the external operation environment, and helps to improve the experimental efficiency and success rate.

[0050] This storage box can be used continuously for a long time once assembled, saving valuable scientific research time. Moreover, this storage box is simple to assemble, the materials are easy to obtain, and it is convenient to carry and transport.

Claims

1. A highly compatible portable oocyte operation needle storage box, characterized in that: include: A box body (1), wherein a cover body (2) is arranged above the box body (1), the box body (1) and the cover body (2) are both rectangular parallelepiped structures, the cover body (2) and the box body (1) are symmetrically arranged up and down, the box body (1) is rotatably connected to one side of the cover body (2), and the box body (1) is buckled and fixed to the other side of the cover body (2); A protective layer (6) is arranged at the bottom of the inner side of the box body (1) and is used to protect the oocyte operation needle; an adhesive tape (8) is arranged at the bottom of the inner side of the box body (1), and the protective layer (6) is adhered and fixed to the box body (1) via the adhesive tape (8); There are two needle placement platforms (7), which are respectively arranged at two ends of the top surface of the protective layer (6) and are used to fix two oocyte operation needles of different lengths.

2. The highly compatible portable oocyte operation needle storage box according to claim 1 is characterized in that: The number of the adhesive strips (8) is two, and the two adhesive strips (8) are arranged in parallel at the inner bottom of the box body (1) along the length direction of the box body (1).

3. The highly compatible portable oocyte operation needle storage box according to claim 1, characterized in that: The protective layer (6) comprises a foam board (9), an adhesive layer (10) and kraft paper (11) which are arranged in sequence from bottom to top; the foam board (9) is bonded and fixed to the box body (1) via an adhesive strip (8); and the foam board (9) and the kraft paper (11) are bonded and connected via the adhesive layer (10).

4. The highly compatible portable oocyte operation needle storage box according to claim 1, characterized in that: The needle placement platform (7) includes double-sided tape 1 (12), double-sided tape 2 (13) and transparent tape (14) which are arranged in sequence from bottom to top and adhered to each other. The double-sided tape 1 (12) is adhered and fixed to the top surface of the protective layer (6), and the adhesive surface of the transparent tape (14) faces upward. The transparent tape (14) is used to fix the oocyte operation needle.

5. The highly compatible portable oocyte operation needle storage box according to claim 1, characterized in that: A buckle seat (3) is fixedly connected to one side of the box body (1), a buckle frame (4) is fixedly connected to one side of the cover body (2), and the buckle seat (3) and the buckle frame (4) are buckled and connected.

6. The highly compatible portable oocyte operation needle storage box according to claim 1, characterized in that: The box body (1) is rotatably connected to the cover body (2) at a side away from the buckle seat (3) and the buckle frame (4) via a hinge shaft (5).

7. The highly compatible portable oocyte operation needle storage box according to claim 3 is characterized in that: The foam board (9) is an EPE pearl cotton foam board.

8. The highly compatible portable oocyte operation needle storage box according to claim 1, characterized in that: The oocyte manipulation needle is a glass capillary needle (15).

9. The highly compatible portable oocyte operation needle storage box according to claim 8, characterized in that: The glass capillary needle (15) comprises a long glass capillary needle (151) and a short glass capillary needle (152), and the long glass capillary needle (151) and the short glass capillary needle (152) are respectively placed horizontally on two needle-setting platforms (7).

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