Low-pressure low-oxygen cell incubator cabin

Abstract

The utility model relates to a low-pressure low-oxygen cell incubator cabin which comprises a sterilization barrel, a culture dish is arranged in the sterilization barrel, a temperature, humidity and carbon dioxide sensor assembly is arranged in the culture dish, an oxygen partial pressure sensor is arranged at one end of the rear side of the sterilization barrel, and an air inlet quick-plug connector assembly is arranged on the rear side of the sterilization barrel. According to the low-pressure low-oxygen cell incubator cabin, the electric tracing band and the heating band are arranged, so that the interior of the sterilization barrel can be kept in a constant-temperature state, the influence of environment temperature change on cell culture is reduced, and the cell metabolism process is promoted; by arranging the temperature and humidity and carbon dioxide sensor assembly, it can be guaranteed that the temperature in the cabin is monitored in real time, and meanwhile the pressure value in the cabin is fed back in real time; by arranging the gas inlet quick-plug connector assembly, in the gas inlet work, treated clean gas is connected through the gas inlet quick-plug connector assembly on the sterilization barrel, the gas content in the matching cavity can be higher, and cross contamination is not prone to occurring.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cabin body of a low-pressure and low-oxygen cell incubator. Background Technique

[0002] An incubator is a key device for carrying out cell culture work. Cell culture technology plays an important role in many fields such as biology, medicine, and pharmacy. During the cell culture process, a stable and suitable environment needs to be provided, including accurately controlling the temperature, humidity, gas components (such as oxygen, carbon dioxide), etc. of the incubator to ensure the normal growth and metabolism of cells.

[0003] Early cell cultures were usually carried out in simple culture utensils with relatively rough condition control. With the progress of technology, the functions of incubators have been continuously improved. Its background technology is mainly reflected in simulating the in-vivo growth environment of cells by controlling culture conditions such as temperature, humidity, and carbon dioxide concentration, so as to study the morphological structure, function, and metabolic process of living cells.

[0004] However, there is insufficient research and development on incubators that simulate special environments in the market, and there are the following several defects: 1. There may be errors in the monitoring and adjustment of carbon dioxide concentration in existing incubators, resulting in unstable culture environments. In addition, parameters such as the temperature and humidity of the incubator may also be affected by the external environment, such as power fluctuations and environmental temperature changes, resulting in unstable culture conditions; 2. The operation and maintenance of incubators are relatively complex, and this complexity may increase operation costs and maintenance costs. At the same time, it may also affect the effect of cell culture and experimental results; 3. High economic cost, the price of incubators is expensive, and certain cost inputs are required for operation and maintenance; 4. There is a risk of microbial contamination during the cell culture process in existing incubators; therefore, a cabin body of a low-pressure and low-oxygen cell incubator is proposed to solve the above problems. Content of the Utility Model

[0005] (1) Technical Problems to be Solved

[0006] Aiming at the deficiencies of the prior art, the utility model provides a cabin body of a low-pressure and low-oxygen cell incubator, which has the advantages of being convenient for accurately controlling the pressure value and oxygen content in the incubator.

[0007] (2) Technical Solutions

[0008] To achieve the above object, the present utility model provides the following technical solutions: A low-pressure and low-oxygen cell culture chamber body, including a sterilization barrel, a culture dish is arranged inside the sterilization barrel, a temperature, humidity and carbon dioxide sensor assembly is arranged inside the culture dish, an oxygen partial pressure sensor is arranged at one end of the rear side of the sterilization barrel, an air inlet quick-connect fitting assembly is arranged at the rear side of the sterilization barrel, a reserved installation hole plug is arranged on the air inlet quick-connect fitting assembly, a plug sealing ring is arranged inside the reserved installation hole plug, a cabin pressure acquisition quick-connect fitting is arranged at the end of the oxygen partial pressure sensor away from the sterilization barrel, an electric tracing heating tape is arranged outside the sterilization barrel, a panel is arranged at the front end of the sterilization barrel, a fixed hinge is connected to one side of the panel, a movable hinge plate is connected to the fixed hinge, a sealing door is arranged on the movable hinge plate, and a sealing ring is connected to the outer wall of the sealing door.

[0009] Further, the quick-connect fitting assembly includes a carbon dioxide inlet quick-connect fitting, a nitrogen inlet quick-connect fitting and an oxygen inlet quick-connect fitting. A carbon dioxide inlet quick-connect fitting is arranged at the rear side of the sterilization barrel, a nitrogen inlet quick-connect fitting is arranged at the rear side of the sterilization barrel, and an oxygen inlet quick-connect fitting is arranged at the rear side of the sterilization barrel.

[0010] Further, a lock rod is arranged on the side of the movable hinge plate away from the fixed hinge, an adjusting screw is arranged on the movable hinge plate, and an adjusting nut is connected to the adjusting screw.

[0011] Further, a spring is arranged on the adjusting screw, a reinforcing gasket is arranged in the middle of the panel, and a bolt is arranged on the movable hinge plate.

[0012] Further, a door panel main adjusting column is connected to the gasket, and the door panel main adjusting column is connected to the middle of the sealing door.

[0013] Further, a bolt hole seat is installed on the side of the panel away from the fixed hinge, a bolt hole seat screw is arranged on the bolt hole seat, and a hinge shaft is arranged between the fixed hinge and the movable hinge plate.

[0014] (III) Beneficial effects

[0015] Compared with the prior art, the technical solution of the present application has the following beneficial effects:

[0016] The cabin of this low - pressure and low - oxygen cell incubator can ensure a constant temperature inside the sterilization barrel by setting up an electric tracing heating belt, so as to reduce the influence of environmental temperature changes on cell culture and promote the cell metabolism process; by setting up a temperature - humidity and carbon dioxide sensor assembly, it can ensure real - time monitoring of the temperature inside the cabin and at the same time provide real - time feedback on the pressure value inside the cabin; by setting up an air inlet quick - connect joint assembly, during the air inlet operation, the treated clean gas can be connected through the air inlet quick - connect joint assembly on the sterilization barrel, making the gas content in the mixing cavity more rapid and not easily cross - contaminated; at the same time, the external sterile gas source is easier to control and easier to maintain a constant temperature, reducing the influence of temperature fluctuations of the gas source inside the cabin. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three - dimensional explosion view of the present utility model;

[0018] Figure 2 It is a three - dimensional explosion view of the partial structure of the present utility model;

[0019] Figure 3 It is a front view of the partial structure of the present utility model;

[0020] Figure 4 It is a top view of the present utility model in the state where the sealing door is closed;

[0021] Figure 5 It is a rear view of the present utility model.

[0022] In the figures: 1 sterilization barrel, 2 culture dish, 3 temperature - humidity and carbon dioxide sensor assembly, 4 oxygen partial pressure sensor, 5 reserved installation hole plug, 6 plug seal ring, 7 in - cabin pressure acquisition quick - connect joint, 8 air inlet quick - connect joint assembly, 801 carbon dioxide air inlet quick - connect joint, 802 nitrogen air inlet quick - connect joint, 803 oxygen air inlet quick - connect joint, 9 electric tracing heating belt, 10 panel, 11 movable hinge plate, 12 lock rod, 13 spring, 14 adjusting screw, 15 adjusting nut, 16 reinforcing gasket, 17 bolt, 18 sealing door, 19 sealing ring, 20 main adjusting column of the door panel, 21 bolt hole seat, 22 bolt hole seat screw, 23 hinge shaft, 24 fixed hinge. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present utility model.

[0024] Please refer to Figures 1-5, the cabin of the low - pressure and low - oxygen cell incubator in this embodiment includes a sterilization barrel 1. Inside the sterilization barrel 1, there is a culture dish 2. Inside the culture dish 2, there is a temperature - humidity and carbon dioxide sensor assembly 3. At one end of the rear side of the sterilization barrel 1, there is an oxygen partial pressure sensor 4. On the rear side of the sterilization barrel 1, there is an air inlet quick - connect fitting assembly 8. On the air inlet quick - connect fitting assembly 8, there is a reserved installation hole plug 5. Inside the reserved installation hole plug 5, there is a plug sealing ring 6. At the end of the oxygen partial pressure sensor 4 away from the sterilization barrel 1, there is an in - cabin pressure acquisition quick - connect fitting 7. Outside the sterilization barrel 1, there is an electric tracing heating tape 9. At the front end of the sterilization barrel 1, there is a panel 10. On one side of the panel 10, there is a fixed hinge 24. Connected to the fixed hinge 24 is a movable hinge plate 11. On the movable hinge plate 11, there is a sealing door 18. On the outer wall of the sealing door 18, there is a sealing ring 19. By setting the air inlet quick - connect fitting assembly 8, the input amounts of carbon dioxide, nitrogen, and oxygen can be controlled to achieve separate control of the three gases. The incubator chamber only contains carbon dioxide, nitrogen, and oxygen, and the sum of the proportions of the three gases is calculated as 100%. Only the contents of two of the gases need to be precisely controlled to control the contents of the three gases.

[0025] Furthermore, the incubator adopts a gentle air circulation mode, making the air circulation close to natural air convection, shortening the recovery time of temperature, humidity, oxygen concentration, and carbon dioxide concentration, and ensuring the balance of temperature, humidity, oxygen concentration, and carbon dioxide concentration; a separate door - temperature control system makes the constant - temperature control inside the box less affected by environmental temperature changes.

[0026] Specifically, on the side of the movable hinge plate 11 away from the fixed hinge 24, there is a locking rod 12. On the movable hinge plate 11, there is an adjusting screw 14. Connected to the adjusting screw 14 is an adjusting nut 15. Connected to the gasket 16 is a main door - panel adjusting column 20. The main door - panel adjusting column 20 is connected to the middle of the sealing door 18. The above structure can install the sealing door 18 on the movable hinge plate 11, thus conveniently controlling the opening and closing of the sealing door 18.

[0027] During implementation, the operations are carried out according to the following steps:

[0028] 1) First, place the cells to be cultured in the culture dish 2, then close the sealing door 18, fix the locking rod 12 on the bolt hole seat 21 to seal the sterilization barrel 1, and control the electric tracing heating tape 9 to heat the inside of the chamber.

[0029] 2) Then connect the processed clean gas pipelines to the carbon dioxide inlet quick - connect fitting 801, nitrogen inlet quick - connect fitting 802, and oxygen inlet quick - connect fitting 803 respectively, and supply gas into the chamber.

[0030] 3) Finally, monitor the in - chamber temperature and gas pressure conditions in real - time through the oxygen partial pressure sensor 4 and the temperature - humidity and carbon dioxide sensor assembly 3.

[0031] In summary, for the cabin of the low-pressure and low-oxygen cell incubator, by setting the electric tracing heating tape 9, the temperature inside the sterilization barrel 1 can be kept constant to reduce the influence of environmental temperature changes on cell culture and promote the cell metabolism process; by setting the temperature, humidity and carbon dioxide sensor assembly 3, the temperature inside the cabin can be monitored in real time and the pressure value inside the cabin can be fed back in real time; by setting the air inlet quick-connect joint assembly 8, during the air inlet operation, the treated clean gas is connected through the air inlet quick-connect joint assembly 8 on the sterilization barrel 1, the gas content in the mixing cavity can be adjusted more quickly and cross-contamination is not likely to occur; and the external sterile gas source is easier to control and the temperature can be controlled more easily, reducing the influence of the temperature fluctuation of the gas source inside the cabin.

[0032] It should be noted that in this text, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising a..." does not exclude the presence of additional identical elements in the process, method, article or device comprising the said element.

[0033] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A low-pressure, low-oxygen cell culture chamber, comprising a sterilization barrel (1), characterized in that: The sterilizing barrel (1) is provided with a culture dish (2), the culture dish (2) is provided with a temperature, humidity and carbon dioxide sensor assembly (3), an oxygen partial pressure sensor (4) is provided at one end of the rear side of the sterilizing barrel (1), an air intake quick plug connector assembly (8) is provided at the rear side of the sterilizing barrel (1), a reserved installation hole plug (5) is provided on the air intake quick plug connector assembly (8), a plug sealing ring (6) is provided inside the reserved installation hole plug (5), and the oxygen partial pressure sensor ( 4) A quick-connect connector (7) for collecting cabin pressure is provided at one end away from the sterilization barrel (1), an electric heating belt (9) is provided on the outside of the sterilization barrel (1), a panel (10) is provided at the front end of the sterilization barrel (1), a fixed hinge (24) is connected to one side of the panel (10), a movable hinge plate (11) is connected to the fixed hinge (24), a sealing door (18) is provided on the movable hinge plate (11), and a sealing ring (19) is connected to the outer wall of the sealing door (18).

2. The low-pressure and low-oxygen cell culture chamber according to claim 1, characterized in that: The quick-connect connector assembly (8) comprises a carbon dioxide intake quick-connect connector (801), a nitrogen intake quick-connect connector (802) and an oxygen intake quick-connect connector (803); the carbon dioxide intake quick-connect connector (801) is arranged on the rear side of the sterilization barrel (1), the nitrogen intake quick-connect connector (802) is arranged on the rear side of the sterilization barrel (1), and the oxygen intake quick-connect connector (803) is arranged on the rear side of the sterilization barrel (1).

3. The low-pressure and low-oxygen cell culture chamber according to claim 1, characterized in that: A locking rod (12) is arranged on the side of the movable hinge plate (11) away from the fixed hinge (24), an adjusting screw (14) is arranged on the movable hinge plate (11), and an adjusting nut (15) is connected to the adjusting screw (14).

4. The low-pressure and low-oxygen cell culture chamber according to claim 3, characterized in that: The adjusting screw (14) is provided with a spring (13), a reinforcing gasket (16) is provided in the middle of the panel (10), and a trigger bolt (17) is provided on the movable hinge plate (11).

5. The low-pressure and low-oxygen cell culture chamber according to claim 4, characterized in that: The gasket (16) is connected to a door panel main adjustment column (20), and the door panel main adjustment column (20) is connected to the middle part of the sealing door (18).

6. The low-pressure and low-oxygen cell culture chamber according to claim 1, characterized in that: A bolt hole seat (21) is installed on the side of the panel (10) away from the fixed hinge (24), and a bolt hole seat screw (22) is arranged on the bolt hole seat (21). A hinge shaft (23) is arranged between the fixed hinge (24) and the movable hinge plate (11).

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