Reaction cup

Abstract

The utility model discloses a reaction cup which comprises a support, a plurality of reaction cup holes are formed in the support and arranged in rows in the length direction of the support, and the outer portion of the side wall, in the length direction, of the support is used for containing a magnetic attraction part. The magnetic attraction part is arranged in the length direction of the support. The plurality of reaction cup holes are arranged in a plurality of rows along the length direction of the bracket, and the plurality of rows of reaction cup holes are arranged along the width direction of the bracket. The device is convenient and fast to use, can reduce the workload of operators, is beneficial to automatic operation, improves the working efficiency, can reduce the use of magnetic bar sleeve consumables, saves the use cost of instruments, and can reduce the risk of cross contamination between reaction cup holes.

Description

Technical Field

[0001] The utility model belongs to the technical field of biomedical detection, and particularly relates to a reaction cup. Background Art

[0002] A reaction cup is a commonly used consumable in biological and medical detection, and is often used for reagent mixing and reaction in a fully automatic reaction instrument. Existing reaction cups are usually single-tube reaction cups and need to be placed in a reaction cup rack to be used. When facing a large number of samples to be detected, operators need to replace and place the reaction cups in the reaction cup rack one by one, which not only increases the workload of the operators, but also is not conducive to improving the automation operation efficiency.

[0003] During high-throughput detection, operators usually use, for example, a 96-well plate to achieve simultaneous detection of multiple samples and improve detection efficiency. However, due to the small volume of a single well in a 96-well plate, generally less than 3 mL (milliliters), the volume of samples (such as nucleic acids) that can be detected and the volume of reagents (such as solvents and / or detergents) added are limited. Therefore, it is impossible to achieve one-time detection of large-volume samples. And because the reaction wells of the 96-well plate are arranged in a 12×8 pattern, when the experimental or detection process involves magnetic bead extraction, a magnetic rod needs to be placed above the sample plate to be tested, and a disposable magnetic rod sleeve is required to achieve the adsorption and / or dissociation of magnetic beads. This not only leads to the risk of cross-contamination between reaction wells, but also increases the use cost of the instrument. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a reaction cup for the deficiencies of the prior art.

[0005] To solve the above technical problems, the utility model adopts the following technical scheme: A reaction cup includes a bracket, and a plurality of reaction cup holes are arranged inside the bracket. The plurality of reaction cup holes are arranged in rows along the length direction of the bracket, and the outer sides of the side walls of the bracket along the length direction are used for placing magnetic attraction parts, and the magnetic attraction parts are arranged along the length direction of the bracket.

[0006] Further, the plurality of reaction cup holes are arranged in multiple rows along the length direction of the bracket, and the multiple rows of reaction cup holes are arranged along the width direction of the bracket.

[0007] Further, the number of the reaction cup holes is two rows.

[0008] Further, the magnetic attraction part includes a first magnet and a second magnet located outside the side walls on both sides of the bracket along the length direction.

[0009] Further, a plurality of accommodation cavities are provided between two adjacent rows of the reaction cup holes, and the plurality of accommodation cavities are arranged in rows along the length direction of the bracket.

[0010] Further, the number of the accommodation cavities is one row, and the number of the accommodation cavities in one row is the same as the number of the reaction cup holes in the corresponding row. Each accommodation cavity is arranged between two reaction cup holes in two adjacent rows along the width direction of the bracket.

[0011] Further, two adjacent reaction cup holes in each row of the reaction cup holes are arranged at intervals, and two adjacent accommodation cavities in one row of the accommodation cavities are arranged at intervals.

[0012] Further, the distance between two adjacent reaction cup holes in each row is equal to the distance between two adjacent reaction cup holes in a 96-well plate.

[0013] Further, the outer surface of the bottom of the reaction cup hole is formed into a curved surface.

[0014] Further, the outer surface of the bottom of the accommodation cavity is formed into a curved surface.

[0015] Further, the inner surface of the bottom of the reaction cup hole and the inner surface of the side wall of the reaction cup hole are in arc transition.

[0016] Further, the inner surface of the bottom of the accommodation cavity and the inner surface of the side wall of the accommodation cavity are in arc transition.

[0017] Further, first steps are arranged in the middle of the two side walls of the bracket along the width direction.

[0018] Further, second steps are arranged at the bottoms of the two side walls of the bracket along the width direction.

[0019] Further, an opening groove is arranged on the side wall of the bracket along the width direction.

[0020] Further, the volume of the reaction cup hole is greater than or equal to 6 milliliters and less than or equal to 18 milliliters.

[0021] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0022] 1. The reaction cup of the present utility model is provided with a plurality of reaction cup holes, and the plurality of reaction cup holes are arranged in rows along the length direction of the bracket. When the magnetic bead method is used for extraction, the magnetic attraction part can be placed outside the side wall along the length direction of the bracket, without immersing the magnetic attraction part into the sample solution, which is convenient and fast, can reduce the workload of operators, is beneficial to automated operation, improves work efficiency, can reduce the use of magnetic rod sleeve consumables, saves the use cost of the instrument, and can reduce the risk of cross-contamination between reaction cup holes.

[0023] 2. The plurality of reaction cup holes of the reaction cup of the present utility model are arranged in multiple rows along the length direction of the bracket, and the update of a plurality of reaction cup holes can be realized through one replacement, making the reaction cup more convenient and efficient to use, further reducing the workload of operators, being more beneficial to automated operation, and further improving work efficiency.

[0024] 3. A plurality of accommodating cavities are arranged between adjacent two rows of reaction cup holes of the reaction cup of the present utility model, and the plurality of accommodating cavities are arranged in rows along the length direction of the bracket, which can facilitate the placement of the target liquid as needed and add the target liquid into the adjacent reaction cup holes.

[0025] 4. The bottom of the reaction cup hole of the reaction cup of the present utility model is set to be curved, which can facilitate the fitting with the reaction cup heating seat of the instrument and facilitate the temperature transfer and precise temperature control of the incubation process of the sample and the reagent.

[0026] 5. The bottom of the reaction cup hole of the reaction cup of the present utility model is in arc transition with the inner wall of the reaction cup hole, which can facilitate the adsorption of magnetic beads from the bottom of the reaction cup hole to the inner wall of the reaction cup hole.

[0027] 6. First steps are arranged in the middle of the two side walls along the width direction of the bracket of the reaction cup of the present utility model, so that the bottom width of the bracket is greater than the top width, which can reduce the mold design difficulty of the reaction cup, and then can reduce the mold cost of the reaction cup, thereby can reduce the product unit price of the reaction cup, and has good economy.

[0028] 7. Second steps are arranged at the bottoms of the two side walls along the width direction of the bracket of the reaction cup of the present utility model, which can facilitate the pressing and fixing of the step surface of the second step of the bracket through elastic pieces and / or ball head plungers, and is also convenient for the placement and removal of the reaction cup.

[0029] 8. An opening groove is arranged on the side wall along the width direction of the bracket of the reaction cup of the present utility model, which can prevent the reaction cup from being reversed when placed in the instrument and ensure the uniqueness of the placement direction of the reaction cup.

[0030] 9. The volume of the reaction cup hole of the reaction cup of the present utility model is greater than or equal to 6 milliliters and less than or equal to 18 milliliters. The single-hole volume of this reaction cup is larger, which can meet the requirements of larger-volume samples and reagents, and can be compatible with the usage scenarios of 96-well plates.

[0031] 10. The reaction cup of the present utility model has a simple structure, is convenient to use, has a wide application range, and is economical. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The top view schematic diagram of a specific embodiment of the reaction cup of the present utility model is shown;

[0033] Figure 2 The cross-sectional view schematic diagram of a specific embodiment of the reaction cup of the present utility model is shown;

[0034] Figure 3 The structural schematic diagram of the bottom of a specific embodiment of the reaction cup of the present utility model is shown;

[0035] Figure 4 The front view schematic diagram of a specific embodiment of the reaction cup of the present utility model is shown;

[0036] Figure 5 The top view schematic diagram of another specific embodiment of the reaction cup of the present utility model is shown;

[0037] Figure 6 The cross-sectional view schematic diagram of another specific embodiment of the reaction cup of the present utility model is shown;

[0038] Figure 7 The structural schematic diagram of the bottom of another specific embodiment of the reaction cup of the present utility model is shown;

[0039] Figure 8 The front view schematic diagram of another specific embodiment of the reaction cup of the present utility model is shown;

[0040] Figure 9 The top view schematic diagram of yet another specific embodiment of the reaction cup of the present utility model is shown;

[0041] Figure 10 The cross-sectional view schematic diagram of yet another specific embodiment of the reaction cup of the present utility model is shown;

[0042] Figure 11 The structural schematic diagram of the bottom of yet another specific embodiment of the reaction cup of the present utility model is shown;

[0043] Figure 12 The front view schematic diagram of yet another specific embodiment of the reaction cup of the present utility model is shown;

[0044] Figure 13 The structural schematic diagram of the relative position change between the reaction cup and the magnetic attraction part in the extraction by magnetic beads method of the present utility model is shown.

[0045] Wherein, 1 - bracket; 11 - side wall in the length direction; 12 - side wall in the width direction; 13 - first step; 14 - second step; 15 - opening groove; 2 - reaction cup hole; 21 - outer surface of the bottom of reaction cup hole 2; 22 - inner surface of the bottom of reaction cup hole 2; 23 - inner surface of the side wall of reaction cup hole 2; 3 - magnetic attraction part; 31 - first magnet; 32 - second magnet; 4 - accommodating cavity; 41 - outer surface of the bottom of accommodating cavity 4; 42 - inner surface of the bottom of accommodating cavity 4; 43 - inner surface of the side wall of accommodating cavity 4. Specific embodiments

[0046] The present utility model will be further described below in conjunction with the embodiments shown in the drawings.

[0047] The direction terms mentioned in the present utility model, such as "inner", "outer", "length direction", "width direction", "middle part", "bottom", etc., are only in the way of referring to the drawings. Therefore, the direction terms used are for explaining and understanding the present utility model, rather than for limiting the present utility model.

[0048] As Figures 1 - 12 shown, the reaction cup of the present utility model includes a bracket 1. A plurality of reaction cup holes 2 are arranged inside the bracket 1, and the plurality of reaction cup holes 2 are arranged in rows along the length direction of the bracket 1. The outside of the side wall 11 of the bracket 1 along the length direction is used for placing the magnetic attraction part 3, and the magnetic attraction part 3 is arranged along the length direction of the bracket 1.

[0049] Among them, the multiple reaction cup holes 2 of the reaction cup are arranged in rows. When using the magnetic beads method for extraction, the magnetic attraction part 3 can be placed outside the side wall 11 of the bracket 1 along the length direction, without immersing the magnetic attraction part 3 into the sample solution, which is convenient and fast, can reduce the work burden of the operator, is beneficial to automated operation, improves work efficiency, and at the same time can reduce the use of magnetic rod sleeve consumables, save the use cost of the instrument, and can reduce the risk of cross-contamination between the reaction cup holes 2.

[0050] In a specific embodiment, as Figures 1 - 3 shown, as Figures 5 - 7 shown, as Figures 9 - 11 shown, the multiple reaction cup holes 2 are arranged in multiple rows along the length direction of the bracket 1, and the multiple rows of reaction cup holes 2 are arranged along the width direction of the bracket 1. Among them, by arranging the multiple reaction cup holes 2 in multiple rows, the update of multiple reaction cup holes 2 (such as 8, 16, 32, and / or 48) can be realized through one replacement, which is more convenient and efficient, further reduces the work burden of the operator, is more beneficial to automated operation, and further improves work efficiency.

[0051] In a specific embodiment, as Figures 1 - 3 shown, as Figures 5 - 7 shown, as Figures 9 - 11 shown, the number of reaction cup holes 2 is two rows, which is convenient and efficient, can reduce the workload of operators, is beneficial to automated operation, improves work efficiency, and can better match the instrument. For example, the number of reaction cup holes 2 in each row is 8 and / or 16, and the number of reaction cup holes 2 in two rows is 16 and / or 32.

[0052] In a specific embodiment, as Figure 1 , Figure 5 , Figure 13 shown, the magnetic attraction part 3 includes a first magnet 31 and a second magnet 32 located outside the two side walls 11 in the length direction of the bracket 1. Among them, by using the first magnet 31 and the second magnet 32, the magnetic beads in the reaction cup holes 2 arranged in rows can be easily adsorbed to the side walls of the reaction cup holes 2, or adsorbed to the side walls and bottom walls of the reaction cup holes 2. For example, both the first magnet 31 and the second magnet 32 are magnets, which have good adsorption effect, simple structure and convenient use.

[0053] In a specific embodiment, as Figures 9 - 11 shown, a plurality of accommodating cavities 4 are arranged between adjacent two rows of reaction cup holes 2, and the plurality of accommodating cavities 4 are arranged in rows along the length direction of the bracket 1, which can facilitate the placement of the target liquid as needed and add the target liquid into the adjacent reaction cup holes 2.

[0054] In a specific embodiment, as Figures 9 - 11 shown, the number of accommodating cavities 4 is one row, and the number of accommodating cavities 4 in one row is the same as the number of reaction cup holes 2 in the corresponding each row. Each accommodating cavity 4 is located between two reaction cup holes 2 in adjacent two rows, which can improve the convenience and orderliness of using the accommodating cavity 4. For example, the number of reaction cup holes 2 in each row is 16, and the number of accommodating cavities 4 in one row is 16. At this time, the number of cup holes of the reaction cup is 48.

[0055] In a specific embodiment, as Figures 1 - 3 shown, as Figures 5 - 7 shown, as Figures 9 - 11 shown, two adjacent reaction cup holes 2 in each row of reaction cup holes 2 are arranged at intervals, and two adjacent accommodating cavities 4 in one row of accommodating cavities 4 are arranged at intervals, which can meet the actual needs, and has a simple structure and convenient use.

[0056] In a specific embodiment, as Figures 1 - 3 shown, as Figures 5 - 7 shown, as Figures 9 - 11As shown, the distance between two adjacent reaction cup holes 2 in each row is equal to the distance between two adjacent reaction cup holes in a 96-well plate, which can be compatible with the usage scenarios of 96-well plates and has a wide application range.

[0057] In a specific embodiment, such as Figure 2 , Figure 3 , Figure 6 , Figure 7 , Figure 10 , Figure 11 As shown, the bottom outer surface 21 of the reaction cup hole 2 is set to a curved surface, which can facilitate the fitting with the reaction cup heating seat of the instrument and facilitate the temperature transfer and precise temperature control in the incubation process of the sample and the reagent.

[0058] In a specific embodiment, such as Figure 2 , Figure 6 , Figure 10 As shown, the bottom inner surface 22 of the reaction cup hole 2 and the inner surface 23 of the side wall of the reaction cup hole 2 are in an arc transition, which can facilitate the adsorption of magnetic beads from the bottom inner surface 22 of the reaction cup hole 2 to the inner surface 23 of the side wall of the reaction cup hole 2.

[0059] In a specific embodiment, such as Figure 10 , Figure 11 As shown, the bottom outer surface 41 of the accommodation cavity 4 is set to a curved surface, which can facilitate the fitting with the reaction cup heating seat of the instrument and facilitate the temperature transfer and precise temperature control in the incubation process of the sample and the reagent.

[0060] In a specific embodiment, such as Figure 10 As shown, the bottom inner surface 42 of the accommodation cavity 4 and the inner surface 43 of the side wall of the accommodation cavity 4 are in an arc transition, which can hold the target liquid, facilitate the transfer of the target liquid, and facilitate production and manufacturing.

[0061] In a specific embodiment, such as Figure 3 , Figure 4 , Figure 7 , Figure 8 As shown, first steps 13 are provided in the middle of both side walls 12 of the bracket 1 along the width direction, such that the bottom width of the bracket 1 is greater than the top width, which can reduce the mold design difficulty of the reaction cup, and thus can reduce the mold cost of the reaction cup, and thereby can reduce the product unit price of the reaction cup, with good economy.

[0062] In a specific embodiment, such as Figure 3 , Figure 4 , Figure 7 , Figure 8 , Figure 11 , Figure 12As shown, second steps 14 are provided at the bottoms of both side walls 12 of the bracket 1 in the width direction, which can facilitate pressing and fixing the step surfaces of the second steps 14 of the bracket 1 through shrapnel and / or ball plungers, and also facilitate the placement and removal of reaction cups.

[0063] In a specific embodiment, as Figure 1 , Figure 3 , Figure 5 , Figure 7 , Figure 9 , Figure 11 As shown, an opening groove 15 is provided on the side wall 12 of the bracket 1 in the width direction, which can prevent the reaction cup from being placed in the instrument in the wrong direction and ensure the uniqueness of the placement direction of the reaction cup. For example, the opening groove 15 is a square groove, which has a simple structure and is convenient to use.

[0064] In a specific embodiment, as Figures 1 - 3 shown, as Figures 5 - 7 shown, as Figures 9 - 11 shown, the volume of the reaction cup hole 2 is greater than or equal to 6 ml and less than or equal to 18 ml. The single-hole volume of this reaction cup is larger, which can meet the needs of larger-volume samples and reagents, and can be compatible with the use scenario of a 96-well plate. For example, the volume of the reaction cup hole 2 can be 6 ml, 9 ml, and / or 18 ml.

[0065] When the present utility model is in use, when processes such as lysis and purification of samples and reagents are carried out in the reaction cup hole 2 without the need for magnetic bead adsorption, the magnetic attraction part 3 is arranged below the reaction cup. At this time, the magnetic attraction part 3 has no effect on the magnetic beads in the reaction cup hole 2. When it comes to the process of removing impurities that requires magnetic bead adsorption, the magnetic attraction part 3 will rise from below the bracket 1 to the outside of the side wall 11 of the bracket 1 along the length direction. At this time, under the action of the magnetic attraction part 3, the magnetic beads in the reaction cup holes 2 arranged in rows are adsorbed to the side wall of the reaction cup hole 2, or are adsorbed to the side wall and the bottom wall of the reaction cup hole 2, which is convenient and fast, can reduce the workload of operators, is conducive to automated operation, improves work efficiency, and can avoid the risk of cross-contamination between reaction cup holes 2. At the same time, it can reduce the use of magnetic rod sleeve consumables and save the use cost of the instrument.

[0066] The protection scope of the present utility model is not limited to the above embodiments. Obviously, those skilled in the art can make various changes and deformations to the present utility model without departing from the scope and spirit of the present utility model. If these changes and deformations belong to the scope of the claims of the present utility model and its equivalent technologies, the intention of the present utility model also includes these changes and deformations.

Claims

1. A reaction cup, characterized in that: The invention comprises a support (1), wherein a plurality of reaction cup holes (2) are arranged inside the support (1), and the plurality of reaction cup holes (2) are arranged in a row along the length direction of the support (1); the outside of the side wall (11) of the support (1) along the length direction is used to place a magnetic attraction part (3), and the magnetic attraction part (3) is arranged along the length direction of the support (1).

2. The reaction cup according to claim 1, characterized in that: The plurality of reaction cup holes (2) are arranged in a plurality of rows along the length direction of the support (1), and the plurality of rows of reaction cup holes (2) are arranged along the width direction of the support (1).

3. The reaction cup according to claim 2, characterized in that: The number of the reaction cup holes (2) is two rows.

4. The reaction cup according to claim 1, characterized in that: The magnetic attraction portion (3) comprises a first magnet (31) and a second magnet (32) located outside two side walls (11) of the bracket (1) along the length direction.

5. The reaction cup according to claim 2, characterized in that: A plurality of accommodating cavities (4) are provided between two adjacent rows of reaction cup holes (2), and the plurality of accommodating cavities (4) are arranged in rows along the length direction of the support (1).

6. The reaction cup according to claim 5, characterized in that: The number of the accommodating cavities (4) is one row, the number of the accommodating cavities (4) in one row is the same as the number of the reaction cup holes (2) in each corresponding row, and each of the accommodating cavities (4) is arranged between two reaction cup holes (2) in two adjacent rows along the width direction of the support (1).

7. The reaction cup according to claim 6, characterized in that: Two adjacent reaction cup holes (2) in each row of reaction cup holes (2) are arranged at intervals, and two adjacent accommodation cavities (4) in a row of accommodation cavities (4) are arranged at intervals.

8. The reaction cup according to claim 7, characterized in that: The distance between two adjacent reaction cup wells (2) in each row is equal to the distance between two adjacent reaction cup wells in a 96-well plate.

9. The reaction cup according to claim 7, characterized in that: The bottom outer surface (21) of the reaction cup hole (2) is configured to be a curved surface, and the bottom outer surface (41) of the accommodating cavity (4) is configured to be a curved surface.

10. The reaction cup according to claim 9, characterized in that: The bottom inner surface (22) of the reaction cup hole (2) and the side wall inner surface (23) of the reaction cup hole (2) are in an arc-shaped transition, and the bottom inner surface (42) of the accommodating cavity (4) and the side wall inner surface (43) of the accommodating cavity (4) are in an arc-shaped transition.

11. The reaction cup according to claim 2, characterized in that: A first step (13) is provided at the middle of the two side walls (12) of the bracket (1) along the width direction.

12. The reaction cup according to claim 2, characterized in that: The bottoms of the two side walls (12) of the bracket (1) along the width direction are both provided with second steps (14).

13. The reaction cup according to claim 2, characterized in that: An opening groove (15) is provided on the side wall (12) of the bracket (1) along the width direction.

14. The reaction cup according to claim 1, characterized in that: The volume of the reaction cup hole (2) is greater than or equal to 6 ml and less than or equal to 18 ml.

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