Device for rapidly capturing and dyeing cells
By designing a device for rapid cell capture and staining, the problems of cell damage and loss in the prior art are solved, rapid and highly selective capture and staining of cells are achieved, and the cells are recovered after staining are allowed, which improves detection efficiency and accuracy.
Patent Information
- Application Number
- CN202421407266.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-19
- Publication Date
- 2025-06-13
- Estimated Expiration
- 2034-06-19
AI Technical Summary
The prior art can easily lead to cell damage and loss during cell smear production, natural drying and fixation, staining and observation, affecting the representativeness of the sample and the accuracy of the analysis results. At the same time, cells cannot be recovered after staining for downstream operations.
A device for rapid cell capture and staining is designed, including a slide vector and a cell capture staining area arranged on the slide vector. Each cell capture staining area consists of a specimen plate, an inlet, an outlet, a capture well and an inner well, achieving rapid and highly selective capture and staining of cells and allowing for cell recovery after staining.
This device realizes rapid cell capture and staining, maintains cell activity and integrity, simplifies the steps of traditional cell staining, shortens the experimental cycle, and provides live cell resources for downstream applications of cells, improving detection efficiency and accuracy.
Smart Images
Figure CN222979225U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of cell capture and staining, and particularly to a device for rapid cell capture and staining. Background Art
[0002] Pathologically or clinically, there are many samples that need to be analyzed for body fluids, including tracheal wash, bronchoalveolar lavage fluid, peritoneal fluid, pleural fluid, synovial fluid, cerebrospinal fluid, vaginal secretion, etc. The cell information contained in these samples is crucial for disease diagnosis, condition monitoring, and treatment efficacy evaluation. For these samples, cell-level staining and observation are required.
[0003] Currently, the staining and observation of cells are carried out by methods of smear preparation, natural drying and fixation, staining, and observation. Specifically, first, the sample needs to be evenly smeared on a highly absorbent glass slide. This process requires the operator to have excellent skills to ensure uniform cell distribution and no damage. Subsequently, the glass slide needs to be naturally dried in a specific temperature and humidity environment and treated with a chemical fixative such as formaldehyde or methanol to terminate the activity of intracellular enzymes. The fixed cells are then labeled by HE staining, immunohistochemical staining, or special staining techniques to facilitate the observation of cell morphology, structure, and specific antigen expression under a microscope. However, this method is prone to physical damage or detachment during the preparation of the smear and the natural drying and fixation steps, resulting in cell loss, affecting the representativeness of the sample and the accuracy of the analysis results. At the same time, in the subsequent staining stage, the true morphology cannot be maintained, there is a long time for staining and observation, and the stained cells cannot be recovered for further downstream operations. Summary of the Invention
[0004] The solution of this application provides a device for rapid cell capture and staining, which can quickly collect cells in the sample and perform subsequent staining identification while maintaining cell viability. Moreover, the stained cells can be recovered and used for downstream detection.
[0005] To achieve the above objectives, the technical solution of this application provides a device for rapid cell capture and staining, including a glass slide carrier; at least one cell capture and staining area provided on the glass slide carrier, where each cell capture and staining area is composed of a specimen plate, an inlet and an outlet that communicate with the specimen plate. Each specimen plate is provided with a plurality of capture holes, and each capture hole is internally provided with an inner hole.
[0006] Compared with the prior art, the technical solution has the following features and beneficial effects:
[0007] 1. Achieve rapid cell capture and maintain cell viability. Through the carefully designed cell capture and staining area, especially the structure of the capture holes and inner holes, the present invention realizes rapid and highly selective capture of cells, ensuring that cells can be captured without undergoing a fixation process, thereby maximizing the preservation of the natural viability and integrity of cells. This design not only reduces cell damage and viability loss but also provides live cell resources for subsequent cell function analysis.
[0008] 2. Achieve convenient staining treatment and recovery. The concave design of the capture area in the device and the connection between the inner hole and the sample outlet not only create an ideal microenvironment for cell staining but also enable the stained cells to be easily recovered through simple hydrodynamic operations. This design simplifies the cumbersome steps of traditional cell staining, shortens the experimental cycle, and retains the recyclability of cells, opening up the way for downstream applications of cells such as gene expression analysis and function testing.
[0009] 3. Improve detection efficiency and accuracy: The large-scale parallel processing of samples is achieved through the setting of multiple capture holes, significantly improving the efficiency of cell detection. Combining the direct connection design of the sample inlet and outlet simplifies the sample loading and processing flow, reduces operation complexity and human error, and improves the repeatability of experiments and the accuracy of results. In addition, the non-destructive collection and staining of cells maintain the natural state of cells, providing more reliable cell morphology and function information for pathology and clinical diagnosis, which helps to improve the accuracy of disease diagnosis and the scientific nature of clinical decision-making. BRIEF DESCRIPTION OF THE DRAWINGS
[0010] The drawings described herein are used to provide a further understanding of the present application, form a part of the present application, and the schematic embodiments and descriptions thereof are used to explain the present application without unduly limiting the present application. In the drawings:
[0011] Figure 1 is a schematic diagram of the overall structure of the device for rapid cell capture and staining provided by this solution.
[0012] Figure 2 is a schematic diagram of the specimen plate of the device for rapid cell capture and staining provided by this solution.
[0013] Figure 3 is a schematic diagram of the capture holes on the specimen plate of the device for rapid cell capture and staining provided by this solution.
[0014] Figure 4 is a schematic diagram of a single cell captured by the capture holes on the specimen plate of the device for rapid cell capture and staining provided by this solution.
[0015] In the figure: 10 - glass slide carrier; 20 - cell capture and staining area; 21 - specimen plate, 211 - capture hole, 2111 - inner hole, 22 - sample inlet, 23 - sample outlet. Detailed implementation manners
[0016] Here, exemplary embodiments will be described in detail, and examples thereof are shown in the drawings. When the following description refers to the drawings, unless otherwise indicated, the same numbers in different drawings represent the same or similar elements. The implementation manners described in the following exemplary embodiments do not represent all implementation manners consistent with one or more embodiments of this specification. On the contrary, they are merely examples of devices and methods consistent with some aspects of one or more embodiments of this specification as detailed in the appended claims.
[0017] It should be noted that: In other embodiments, the steps of the corresponding methods are not necessarily executed in the order shown and described in this specification. In some other embodiments, the steps included in the method may be more or less than those described in this specification. In addition, a single step described in this specification may be decomposed into multiple steps for description in other embodiments; and multiple steps described in this specification may also be combined into a single step for description in other embodiments.
[0018] Embodiment 1
[0019] This solution provides a device for rapid cell capture and staining. The device for rapid cell capture and staining is applicable to capturing and staining cells in a sample, breaking through many problems brought about by the traditional cell staining and observation, which require fixing cells, drying cells, and staining cells. It can rapidly capture cells without additional cell fixation, can perform subsequent staining identification while maintaining the cell viability state, and the stained cells can be recycled and downstream detected through a simple cell picking device.
[0020] As Figure 1 shown, the device for rapid cell capture and staining provided by this solution includes:
[0021] A glass slide carrier 10;
[0022] At least one cell capture and staining area 20 provided on the glass slide carrier 10, where each cell capture and staining area 20 is composed of a specimen plate 21, a sample inlet 22 and a sample outlet 23 that are in communication with the specimen plate 21. A plurality of capture holes 211 are provided on each specimen plate 21, and an inner hole 2111 is provided inside each capture hole 211.
[0023] Inject the cell suspension into the specimen plate 21 through the injection port 22, capture the cells by means of the capture holes 211 provided in the specimen plate 21, and other liquids can flow out through the inner holes 2111 to the outlet port 23; when staining is required, inject the staining agent into the specimen plate 21 to achieve cell staining, so that the device for rapid cell capture and staining can achieve rapid cell capture and convenient staining at the same time.
[0024] Multiple cell capture and staining areas 20 can be provided on the glass slide carrier 10, and each cell capture and staining area 20 can independently capture and perform subsequent staining on the cells in the injected cell suspension. The specific number of cell capture and staining areas 20 is determined according to the glass slide carrier 10.
[0025] In Figure 1 In the shown example, two cell capture and staining areas 20 arranged side by side are provided on the glass slide carrier 10. The glass slide carrier 10 is selected to be of a size suitable for scanning by a scanner. The thickness of the glass slide carrier 10 is 10 mm, and the size of each cell capture and staining area 20 is 15 * 15 mm.
[0026] As Figure 2 As shown, each cell capture and staining area 20 includes a specimen plate 21 placed on the glass slide carrier, wherein the injection port 22 and the outlet port 23 are respectively located on different sides of the specimen plate 21, and the injection port 22 and the outlet port 23 are in communication with the specimen plate 21. The advantage of such a setting is that the cell suspension 22 can enter the specimen plate 21 from the injection port 22 and then the liquid flows out from the outlet port 23.
[0027] Preferably, the injection port 22 and the outlet port 23 are respectively located on opposite sides of the specimen plate 21. The advantage of such a setting is that the entering liquid can flow through as many areas of the specimen plate 21 as possible.
[0028] In some embodiments, the height of the injection port 22 is not lower than the top plane position of the capture holes 211 on the specimen plate 21, and the height of the outlet port 23 is not higher than the bottom plane position of the inner holes 2111. The advantage of such a setting is that it is convenient for the liquid to flow out due to its own weight from top to bottom.
[0029] In some embodiments, both the injection port 22 and the outlet port 23 are channels directly in communication with the glass slide carrier 10. The diameters of the injection port 222 and the outlet port 23 are 50 - 100 μm. In a specific embodiment, the injection port 22 and the outlet port 23 are channels with a diameter of 100 μm.
[0030] To make the liquid on the specimen plate 21 flowable, multiple capture holes 211 on the specimen plate 21 form a capture area, which is recessed toward the bottom side relative to the top plane of the specimen plate 21. The sample inlet 22 is communicated with the capture area of the specimen plate 21 and the height of the sample inlet 22 is higher than the height of the capture area. In some embodiments, the multiple capture holes 211 are arranged in the middle area of the specimen plate 21.
[0031] In addition, multiple capture holes 211 are provided on the specimen plate 21 of this solution, and the gap between the capture holes 211 is smaller than the diameter of a single cell, so as to prevent the cell suspension from being adhesively attached to the positions between the capture holes 211 when flowing through each capture hole 211.
[0032] In some embodiments, the aperture size of the capture hole 211 is 10 - 50um, and the depth of the capture hole 211 is 20 - 40um. Preferably, the aperture size of the capture hole 211 is 20 - 30um, and the depth of the capture hole 211 is 20 - 30um. As Figure 3 shown, generally the diameter of a single cell is 8 - 15um, so when the cell suspension flows through the capture hole 211, the cells can be captured.
[0033] It should be noted that the number of remaining cells after the pretreatment of the cell suspension in this solution is less than the number of capture holes 211. When using the capture holes 211 to capture cells, it is not necessary to ensure that each capture hole 211 contains a single cell. Even if there are multiple cells in the same capture hole 211, it will not affect staining and observation.
[0034] In some embodiments, the multiple capture holes 211 on the specimen plate 21 of this solution are uniformly arranged in an array. Specifically, the connecting lines formed by the multiple capture holes 211 in the horizontal direction are parallel, and the connecting lines formed by the multiple capture holes 211 in the vertical direction are parallel. Preferably, the aperture of each capture hole 211 is the same, and the distance between every two capture holes 211 is the same. The advantage of this setting is that: when the cell suspension is injected into the specimen plate 21, the cell dropping rate will also increase greatly, reducing the influence that air or other liquids may be present in the capture holes 211 and are not easily discharged, resulting in cells not being able to drop into the holes.
[0035] In addition, an inner hole 2111 is provided at the bottom of each capture hole 211, where the diameter of the inner hole 2111 is smaller than the diameter of the capture hole 211, and the bottom of the inner hole 2111 is communicated with the sample outlet 23. The setting of this inner hole 2111 is to avoid the loss of cells while removing waste liquid.
[0036] The washing solution enters from the sample inlet and exits from the sample outlet. The cells do not cover all the inner holes 2111, so the cells are in a relatively movable state in the capture holes 211. However, the washing solution flows unidirectionally, and the liquid only goes in the direction of the sample outlet.
[0037] In some embodiments, the waste liquid can also be extracted by sucking the washing liquid through negative pressure at the position of the sample outlet. At this time, the sample outlet is blocked during the washing liquid washing and incubation processes, so as to ensure that the washing liquid fills the entire cell area. Then, when the washing liquid needs to be discharged, the washing liquid is sucked away from the sample outlet by negative pressure.
[0038] Similarly, for the convenience of liquid export, the bottom plane formed by the inner hole 2111 of the capture area is recessed toward the top side relative to the bottom of the specimen plate 21, so that the bottom plane of the inner hole 2111 is higher than the height of the sample outlet 23.
[0039] The diameter of the inner hole 2111 in this solution is smaller than the diameter of a single cell. Specifically, the diameter of the inner hole 2111 is 0.1 - 10 μm, and preferably, the diameter of the inner hole 2111 is 3 - 5 μm.
[0040] The capture hole 211 contains at least two inner holes 2111. In some embodiments, the capture hole 211 contains three inner holes 2111, and the three inner holes 2111 are arranged in three directions.
[0041] In addition, a closing cover (not shown in the figure) with an opening and closing setting is provided on the sample outlet 23 of the device for rapid cell capture and staining provided by this solution. When it is necessary to incubate the cells in the capture hole 211, the closing cover can be closed to seal the sample outlet 23, and when it is necessary to wash, the sample outlet 23 is opened so that the liquid can directly drain from the inner hole 2111.
[0042] In summary, the device for rapid cell capture and staining provided by this solution can achieve rapid capture of cell samples and subsequent staining. It is not necessary to fix the cells in the wells with a fixing solution before staining. At the same time, since the cells are separated in individual wells after staining, single-cell recovery can be achieved. The usage method of this device is as follows:
[0043] When it is necessary to capture cells, the cell suspension is injected into the specimen plate 21 from the injection port 22, and the cells in the cell suspension are captured by the capture holes 211; when it is necessary to incubate the cells, the sample outlet 23 is closed and the incubation solution is injected into the specimen plate 21 from the injection port 22 to incubate the cells; when it is necessary to stain the cells, the staining agent is injected into the specimen plate 21 from the injection port 22 to stain the cells in the capture holes 211; when it is necessary to wash the cells, the sample outlet 23 is opened and the washing solution is injected into the specimen plate 21 from the injection port 22, and the waste liquid flows out from the sample outlet 23.
[0044] Those skilled in the art should understand that the technical features of the above embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as within the scope described in this specification.
[0045] The above embodiments only express several implementation manners of the present application, and the description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the present application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several deformations and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.
Claims
1. A device for rapid cell capture and staining, characterized in that: include: Slide carrier (10); At least one cell capture staining area (20) is arranged on a glass slide carrier (10), wherein each cell capture staining area (20) is composed of a specimen plate (21) and an inlet (22) and an outlet (23) connected to the specimen plate (21), each specimen plate (21) is provided with a plurality of capture holes (211), and each capture hole (211) is provided with an inner hole (2111).
2. The device for rapid cell capture and staining according to claim 1, characterized in that: A plurality of capture holes (211) on the specimen plate (21) form a capture zone, which is recessed toward the bottom relative to the top plane of the specimen plate (21); the injection port (22) is connected to the capture zone of the specimen plate (21) and the height of the injection port (22) is higher than the height of the capture zone.
3. The device for rapid cell capture and staining according to claim 2, characterized in that: The bottom plane formed by the inner hole (2111) of the capture zone is recessed toward the top side relative to the bottom of the specimen plate (21), and the bottom plane of the inner hole (2111) is higher than the height of the sample outlet (23).
4. The device for rapid cell capture and staining according to claim 1, characterized in that: The aperture size of the capture hole (211) is 10-50 um, and the depth of the capture hole (211) is 20-40 um.
5. The device for rapid cell capture and staining according to claim 1, characterized in that: The gap between the capture holes (211) and the capture holes (211) is smaller than the diameter of a single cell.
6. The device for rapid cell capture and staining according to claim 1, characterized in that: The multiple capture holes (211) on the specimen plate (21) are evenly arranged in an array.
7. The device for rapid cell capture and staining according to claim 1, characterized in that: An inner hole (2111) is provided at the bottom of each capture hole (211), wherein the diameter of the inner hole (2111) is smaller than the diameter of the capture hole (211), and the bottom of the inner hole (2111) is connected to the sample outlet (23).
8. The device for rapid cell capture and staining according to claim 1, characterized in that: The diameter of the inner hole (2111) is 0.1-10um.
9. The device for rapid cell capture and staining according to claim 1, characterized in that: The sample inlet (22) and the sample outlet (23) are both channels directly connected to the glass slide carrier (10), and the diameters of the sample inlet (22) and the sample outlet (23) are 50-100 um.
10. The device for rapid cell capture and staining according to claim 1, characterized in that: The sample outlet (23) is provided with a closing cover which is opened and closed.