Orifice plate mechanism for organoid sphere culture

Abstract

The utility model discloses a pore plate mechanism for organoid sphere culture, and relates to the technical field of cell culture, the pore plate mechanism comprises a pore plate base, the peripheral edge of the upper end of the pore plate base is provided with a limiting groove, a pore plate cover is inserted in the limiting groove, the lower end of the pore plate cover is fixedly provided with a sealing gasket, and the sealing gasket is provided with a sealing groove. The lower end of the sealing gasket abuts against the interior of the limiting groove, a humidity control assembly is arranged at the upper end of the pore plate cover, a target humidity value needing to be maintained is set on the controller according to experimental requirements and characteristics of a biological sample, and the controller continuously monitors an actual humidity value fed back by the humidity sensor and compares the actual humidity value with the set target humidity value; when the actual humidity is lower than the set target humidity value, the controller sends a signal to the atomization humidifier, the atomization humidifier is started to increase the humidity in the incubator, the binding force between cells and the stability of spheres can be kept, and sphere depolymerization or deformation caused by dryness is prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, and particularly relates to a well plate mechanism for culturing organoid spheres. Background Art

[0002] An organoid refers to a small three-dimensional tissue structure cultured in vitro, which can simulate some functions and characteristics of real organs. These organoids are usually self-assembled from stem cells or somatic cells under appropriate culture conditions, and can include various different types of cells, forming a miniature model similar to a real organ.

[0003] Most organoid sphere cultures use well plate mechanisms. The culture of some organoids requires appropriate humidity, but the existing well plate mechanisms do not have the function of controlling humidity, resulting in the well plate mechanisms being unable to provide a more suitable culture environment. Based on this, this solution provides a well plate mechanism for culturing organoid spheres to solve the above-mentioned problems. Summary of the Utility Model

[0004] To solve the above technical problems, a well plate mechanism for culturing organoid spheres is provided. This technical solution solves the problem that most organoid sphere cultures use well plate mechanisms, the culture of some organoids requires appropriate humidity, but the existing well plate mechanisms do not have the function of controlling humidity, resulting in the well plate mechanisms being unable to provide a more suitable culture environment as mentioned in the above background art.

[0005] To achieve the above purposes, the technical solution adopted by the utility model is as follows: A well plate mechanism for culturing organoid spheres, including a well plate base, wherein limiting grooves are opened at the upper peripheral edges of the well plate base, a well plate cover is inserted into the limiting grooves, a sealing gasket is fixedly installed at the lower end of the well plate cover, the lower end of the sealing gasket abuts against the inside of the limiting grooves, and a humidity control component is arranged at the upper end of the well plate cover;

[0006] Among them, the humidity control component includes an atomizing humidifier, the atomizing humidifier is fixedly installed on the upper right side of the well plate cover, the output end of the atomizing humidifier is fixedly communicated with a first conduit, the other end of the first conduit penetrates through the upper top plate of the well plate cover and is fixedly communicated with a second conduit, a plurality of third conduits are fixedly communicated with the surface of the second conduit, and a plurality of through holes are opened at the lower ends of the plurality of third conduits.

[0007] Preferably, mounting blocks are fixedly connected to the left and right sides of the inner side wall at the upper end of the well plate cover, the mounting blocks are sleeved on the surface of the third conduits, and a humidity sensor is arranged in the middle of the inner wall at the rear end of the well plate cover.

[0008] Preferably, a plurality of cavities are opened at the upper end of the well plate base, and a plurality of matching culture well units are arranged inside the plurality of cavities.

[0009] Preferably, handles are fixedly installed on both the left and right sides of the upper end of the orifice plate cover, and a controller is provided on the front side of the upper end of the orifice plate cover.

[0010] Preferably, support legs are fixedly installed at the four corners of the lower end of the orifice plate base, and anti-slip pads are fixedly installed at the lower ends of the support legs.

[0011] Compared with the prior art, the present utility model provides an orifice plate mechanism for culturing organoid spheres, having the following beneficial effects:

[0012] According to the requirements of the experiment and the characteristics of the biological sample, a target humidity value to be maintained is set on the controller. The controller continuously monitors the actual humidity value fed back by the humidity sensor and compares it with the set target humidity value. When the actual humidity is lower than the set target humidity value, the controller sends a signal to the atomizing humidifier to start the atomizing humidifier to increase the humidity in the incubator, which can help maintain the binding force between cells and the stability of the spheres and prevent the spheres from depolymerizing or deforming due to drying. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 is a three-dimensional structural schematic diagram of the present utility model;

[0014] Figure 2 is an unfolded structural schematic diagram of the present utility model;

[0015] Figure 3 is a structural schematic diagram of the humidity control component of the present utility model;

[0016] Figure 4 is a structural schematic diagram of the lower end of the cover plate of the present utility model.

[0017] The reference numerals in the figures are:

[0018] 1, orifice plate base; 2, support leg; 3, anti-slip pad; 4, limiting groove; 5, orifice plate cover;

[0019] 6, humidity control component; 601, atomizing humidifier; 602, first conduit; 603, second conduit; 604, mounting block; 605, third conduit; 606, through hole; 607, humidity sensor;

[0020] 7, handle; 8, controller; 9, cavity; 10, culture well unit; 11, sealing gasket. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] The following description is used to disclose the present utility model so that those skilled in the art can implement the present utility model. The following preferred embodiments are only examples, and those skilled in the art can think of other obvious variations.

[0022] Refer to Figures 1-4 As shown, a well plate mechanism for culturing organoid spheres includes a well plate base 1. Limit grooves 4 are provided at the upper peripheral edges of the well plate base 1. A well plate cover 5 is inserted into the inside of the limit grooves 4. A sealing gasket 11 is fixedly installed at the lower end of the well plate cover 5. The lower end of the sealing gasket 11 abuts against the inside of the limit grooves 4. The sealing gasket 11 can stably maintain the environmental conditions inside the well plate mechanism, which helps the normal growth and development of organoid spheres. A humidity control component 6 is provided at the upper end of the well plate cover 5. The humidity control component 6 includes an atomizing humidifier 601. The atomizing humidifier 601 is fixedly installed on the upper right side of the well plate cover 5. The atomizing humidifier 601 transports steam through a through hole 606 into the inside of the well plate cover 5 through a first conduit 602, a second conduit 603, and a third conduit 605 to increase the humidity. The output end of the atomizing humidifier 601 is fixedly connected to a first conduit 602. The other end of the first conduit 602 penetrates through the upper top plate of the well plate cover and is fixedly connected to a second conduit 603. A number of third conduits 605 are fixedly connected to the surface of the second conduit 603. A number of through holes 606 are provided at the lower ends of the number of third conduits 605.

[0023] Refer to Figure 1 、 Figure 3 and Figure 4 As shown, mounting blocks 604 are fixedly connected to the left and right sides of the inner side wall at the upper end of the well plate cover 5. The mounting blocks 604 are sleeved on the surface of the third conduit 605. A humidity sensor 607 is provided in the middle of the inner wall at the rear end of the well plate cover 5. The humidity sensor 607 can be a humidity sensor 607 with the model: Sensirion SHTC3, which can measure and reflect the current humidity level in real time. A number of cavities 9 are provided at the upper end of the well plate base 1. A number of matching culture well units 10 are provided inside the number of cavities 9. Handles 7 are fixedly installed on the left and right sides at the upper end of the well plate cover 5. A controller 8 is provided at the front side of the upper end of the well plate cover 5. The controller 8 is electrically connected to the humidity sensor 607 and the atomizing humidifier 601. The controller can be a humidity sensor with the model: FLZ600. Any humidity sensor 607 that can achieve this function in the prior art can be used without limitation. The humidity controller 8 uses the real-time data provided by the humidity sensor 607 to adjust and maintain the humidity by controlling the operation of the atomizing humidifier 601, so as to ensure that the organoid spheres are cultured and grown under the most suitable humidity conditions. Support legs 2 are fixedly installed at the four corner positions at the lower end of the well plate base 1. Anti-slip pads 3 are fixedly installed at the lower ends of the support legs 2. The anti-slip pads 3 can increase the friction between the well plate mechanism and the working surface, prevent sliding during operation, and help maintain the stability of the culture environment.

[0024] Working principle and usage process of the present utility model: During use, place the culture well unit 10 inside the cavity 9, insert the well plate cover 5 into the limiting groove 4 opened at the upper end of the well plate base 1, set the target humidity value to be maintained on the controller 8 according to the requirements of the experiment and the characteristics of the biological sample. The controller 8 continuously monitors the actual humidity value fed back by the humidity sensor 607 and compares it with the set target humidity value. When the actual humidity is lower than the set target humidity value, the controller 8 sends a signal to the atomizing humidifier 601, and the atomizing humidifier 601 transports steam through the through hole 606 into the inside of the well plate cover 5 through the first conduit 602, the second conduit 603, and the third conduit 605 to increase the humidity inside the well plate mechanism, which can help maintain the binding force between cells and the stability of the spheres, and prevent the spheres from depolymerizing or deforming due to drying. When the actual humidity approaches the target humidity value, the controller 8 will correspondingly reduce the operation of the atomizing humidifier 601 to prevent the humidity from exceeding the set target humidity value and avoid the reduction of the oxygen content in the air caused by excessive humidity, which affects the health and growth rate of cells or tissues. By adjusting the humidity, the culture conditions of the organoid spheres can be optimized to the greatest extent, ensuring the health of the cells and the stability of the spheres.

[0025] The above shows and describes the basic principle, main features and advantages of the present utility model. Those skilled in the art should understand that the present utility model is not limited by the above embodiments. What is described in the above embodiments and the specification is only the principle of the present utility model. Without departing from the spirit and scope of the present utility model, various changes and improvements will occur to the present utility model, and these changes and improvements all fall within the scope of the present utility model claimed. The scope of protection required by the present utility model is defined by the appended claims and their equivalents.

Claims

1. A well plate mechanism for organoid spheroid culture, characterized in that: The orifice plate base (1) comprises a limiting groove (4) provided at the edges of the upper end of the orifice plate base (1), a orifice plate cover (5) is inserted into the limiting groove (4), a sealing gasket (11) is fixedly installed at the lower end of the orifice plate cover (5), the lower end of the sealing gasket (11) abuts against the inside of the limiting groove (4), and a humidity control component (6) is provided at the upper end of the orifice plate cover (5); The humidity control component (6) comprises an atomizing humidifier (601), which is fixedly mounted on the right side of the upper end of the orifice plate cover (5); the output end of the atomizing humidifier (601) is fixedly connected to a first conduit (602); the other end of the first conduit (602) passes through the upper top plate of the orifice cover and is fixedly connected to a second conduit (603); the surface of the second conduit (603) is fixedly connected to a plurality of third conduits (605); and the lower ends of the plurality of third conduits (605) are each provided with a plurality of through holes (606).

2. A well plate mechanism for organoid spheroid culture according to claim 1, characterized in that: The left and right sides of the inner wall at the upper end of the orifice plate cover (5) are fixedly connected with mounting blocks (604), and the mounting blocks (604) are sleeved on the surface of the third conduit (605). A humidity sensor (607) is provided in the middle of the inner wall at the rear end of the orifice plate cover (5).

3. A well plate mechanism for organoid spheroid culture according to claim 1, characterized in that: A plurality of cavities (9) are provided at the upper end of the well plate base (1), and a plurality of matching culture well units (10) are provided inside the plurality of cavities (9).

4. A well plate mechanism for organoid spheroid culture according to claim 1, characterized in that: Handles (7) are fixedly mounted on both left and right sides of the upper end of the orifice plate cover (5), and a controller (8) is provided on the front side of the upper end of the orifice plate cover (5).

5. The well plate mechanism for organoid spheroid culture according to claim 1, characterized in that: Support legs (2) are fixedly mounted at the four corners of the lower end of the orifice plate base (1), and anti-slip pads (3) are fixedly mounted at the lower ends of the support legs (2).

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