Intelligent storage cell incubator
By introducing intelligent storage design and storage turret structure into the cell incubator, the problems of low space utilization, inconvenient operation and poor gas flowability in the existing technology are solved, and a more efficient cell culture environment and convenient operation are achieved.
Patent Information
- Application Number
- CN202421490192.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-26
- Publication Date
- 2025-07-01
- Estimated Expiration
- 2034-06-26
AI Technical Summary
The partition structure of the existing cell incubator results in low space utilization, inconvenient operation, poor gas flowability and uneven temperature and humidity distribution.
An intelligent storage cell incubator is designed, using a motor-driven storage turret rotation mechanism, combining a turret composed of an annular top disk, an annular chassis, a vertical pole and a fork-shaped placement frame to achieve hollow transparency up, down, left, left, right, front and back, and facilitate gas flow and uniform distribution of temperature and humidity.
It improves the convenience of storage and access of culture bottles, enhances gas flowability and uniform temperature and humidity distribution, provides a better cell culture environment, and improves the efficiency and yield of the cell incubator.
Smart Images
Figure CN223047531U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of cell culture equipment, and particularly relates to an intelligent warehousing cell culture incubator. Background Technique
[0002] The statements in this part only provide background technical information related to the utility model, and do not necessarily constitute prior art.
[0003] In the field of cell biology, a cell culture incubator can simulate the physiological environment of tissues in vivo and culture and proliferate cells in vitro. The cell culture incubator provides environmental conditions for maintaining the growth, proliferation and physiological state of cells, such as temperature, humidity, gas concentration (such as carbon dioxide), and other conditions required for cell growth.
[0004] In the existing technical solutions, the cell culture incubator often adopts a partitioned structure. The cabin body is divided into upper and lower layers by metal partitions, and cell culture bottles are placed on the partitions for cell culture. Such a structure results in low space utilization and inconvenient operation. For example, after the culture bottles are placed on the partitions, taking and placing the inner culture bottles requires moving the culture bottles at the outer door first, which is cumbersome, time-consuming, and will affect the working environment state in the incubator and increase energy consumption. Moreover, the gas flowability is poor and the temperature and humidity distribution is uneven. Summary of the Utility Model
[0005] In order to solve the above problems, the utility model provides an intelligent warehousing cell culture incubator, which not only drives the storage turret to rotate by the rotation of the output shaft of the motor, facilitating the access of culture bottles; moreover, a turret is composed of an annular top plate, an annular bottom plate, vertical rods and a plurality of fork-shaped placement frames, making the entire turret hollow and transparent up and down, left and right, front and back, facilitating gas flow and uniform distribution of temperature and humidity, and providing a good culture environment for the culture bottles placed on the turret.
[0006] According to some embodiments, the utility model adopts the following technical solutions:
[0007] In a first aspect, an intelligent warehousing cell culture incubator is provided, including a motor, a transmission device, a first bearing, a box body with a through hole at the bottom, and a storage turret arranged in the box body. The storage turret includes a central axis, an annular top plate, an annular bottom plate, vertical rods and fork-shaped placement frames;
[0008] The vertical rods are uniformly arranged between the annular top plate and the annular bottom plate, and a plurality of fork-shaped placement frames are uniformly arranged on the vertical rods from top to bottom. The fork-shaped placement frames are used for placing cell culture bottles;
[0009] The top end of the central axis is fixedly connected to the center of the annular chassis. The bottom end of the central axis passes through the through hole and extends out of the box body. The first bearing is arranged in the through hole. The outer ring of the first bearing is fixedly connected to the box body, and the central axis is fixedly connected to the inner ring of the first bearing;
[0010] The output shaft of the motor is connected to the central axis through the transmission device to drive the storage turret to rotate.
[0011] Further, it further includes a support frame and a second bearing;
[0012] The bottom end of the central axis is fixedly connected to the inner ring of the second bearing, and the outer ring of the second bearing is fixedly connected to the support frame.
[0013] Further, the connection part of the transmission device and the central axis is located between the first bearing and the second bearing.
[0014] Further, both the annular top plate and the annular chassis include an outer ring and a plurality of tooth parts uniformly distributed on the outer side of the outer ring, and there are two vertical rods arranged between each tooth part of the annular top plate and one tooth part of the annular chassis.
[0015] Further, two semi-circular water trays are placed at the bottom inside the box body, and a groove adapted to the central axis is provided in the middle of the straight edge of the water tray.
[0016] Further, heating wires are arranged on the side wall of the box body.
[0017] Further, a plurality of air flow heat conduction fans are arranged on the upper wall of the box body, and the air flow heat conduction fans are arranged directly above the storage turret.
[0018] Further, it further includes a gas cylinder filled with gas and an air pump connected to the gas cylinder;
[0019] An air inlet hole is provided at the top of the box body, and the air inlet hole is connected to the air pump.
[0020] Further, it further includes a bottom plate, four support columns and four universal wheels;
[0021] The four corners at the bottom of the box body are respectively fixedly connected to the first ends of the four support columns, the second ends of the four support columns are respectively fixedly connected to the four corners on the top surface of the bottom plate, and a universal wheel is fixedly arranged at each of the four corners on the bottom surface of the bottom plate.
[0022] Further, the box body is provided with a box door, and a glass window is provided in the middle of the box door.
[0023] Compared with the prior art, the beneficial effects of the present utility model are:
[0024] An intelligent warehousing cell culture incubator provided by the utility model drives the storage and rotation tower to rotate by the rotation of the output shaft of the motor, facilitating the access of culture bottles.
[0025] An intelligent warehousing cell culture incubator provided by the utility model uses an annular top plate, an annular bottom plate, vertical rods and a plurality of fork-shaped placement racks to form a rotating tower, making the entire rotating tower hollow and transparent up and down, left and right, front and back, facilitating gas flow and uniform distribution of temperature and humidity, and providing a good culture environment for the culture bottles placed on the rotating tower. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] The specification drawings forming a part of the present utility model are used to provide a further understanding of the present utility model. The schematic embodiments of the present utility model and their descriptions are used to explain the present utility model and do not constitute an improper limitation to the present utility model.
[0027] Figure 1 is the overall structural schematic diagram of an intelligent warehousing cell culture incubator of the present utility model;
[0028] Figure 2 is the structural diagram of the housing of the present utility model;
[0029] Figure 3 is the structural diagram of the storage and rotation tower of the present utility model;
[0030] Figure 4 is the hardware connection diagram of an intelligent warehousing cell culture incubator of the present utility model. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0031] The following further illustrates the present utility model in conjunction with the drawings and embodiments.
[0032] It should be noted that the following detailed descriptions are all illustrative and are intended to provide further explanations of the present utility model. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present utility model belongs.
[0033] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present utility model. As used herein, unless otherwise clearly specified in the context, the singular form is also intended to include the plural form. In addition, it should be understood that when the term "comprising" is used in this specification, it indicates the presence of features, steps, operations, devices, components, and / or combinations thereof.
[0034] In the present utility model, terms such as "upper", "vertical", "horizontal", "upward", "downward", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only relational terms determined for the convenience of describing the structural relationship of each component or element of the present utility model, and do not specifically refer to any component or element in the present utility model, and should not be construed as a limitation to the present utility model.
[0035] In the present utility model, terms such as "fixed" and "fixedly installed" should be understood in a broad sense, which may mean a fixed connection, an integral connection or a detachable connection. For relevant scientific research or technical personnel in this field, the specific meanings of the above terms in the present utility model can be determined according to specific circumstances, and should not be construed as a limitation to the present utility model.
[0036] This embodiment provides an intelligent warehousing cell incubator.
[0037] An intelligent warehousing cell incubator provided by this embodiment, as Figure 1 shown, includes: a housing 1, a motor 12, a transmission device (transmission chain) 13, a heating mechanism (heating device), an air flow heat conduction fan 10, a support frame 11, a gas device, a second bearing 16, and a storage turret (abbreviation, turret) 2, an environmental monitoring sensor 4 and a humidifying device 3 arranged inside the housing 1.
[0038] As Figure 2 shown, the housing 1 includes a box body 5, a box door 6, a bottom plate 8, four support columns 7 and four universal wheels 9.
[0039] As Figure 2 shown, the box body 5 is a rectangular box body with an opening on one side, and a switchable box door 6 is arranged on the opening side of the box body 5.
[0040] As an implementation manner, a rectangular installation opening is formed through the middle of the box door 6 for installing glass, which is convenient for experimental personnel to observe the situation inside the box body.
[0041] The four corners of the bottom of the box body 5 are respectively fixedly connected to the first ends of the four support columns 7, and the second ends of the four support columns 7 are respectively fixedly connected to the four corners of the top surface of the bottom plate 8; a universal wheel 9 is fixedly arranged at each of the four corners of the bottom surface of the bottom plate 8.
[0042] As Figure 3 shown, the support frame 11 includes an upper top plate and a lower bottom plate and several columns arranged between the upper top plate and the lower bottom plate. The lower bottom plate is fixed on the top surface of the bottom plate 8. The motor 12 is fixed on the upper top plate, and the output shaft of the motor 12 is perpendicular to the upper top plate. An installation hole is formed in the upper top plate.
[0043] As Figure 3 shown, the number of columns is 6.
[0044] AsFigure 3 As shown, the storage turret 2 includes a central shaft 14, a gear 15, an annular top plate 17, an annular bottom plate 18, 24 vertical rods 19, and at least 108 fork-shaped placement racks 20.
[0045] The annular top plate 17 includes an outer ring and 12 tooth portions evenly distributed on the outer side of the outer ring.
[0046] The annular bottom plate 18 includes an outer ring and 12 tooth portions evenly distributed on the outer side of the outer ring. Each tooth portion of the annular bottom plate 18 can be a fork-shaped placement rack. The outer ring of the annular bottom plate 18 has the same size as the outer ring of the annular top plate 17.
[0047] Between each tooth portion of the annular top plate 17 and one of the tooth portions of the annular bottom plate 18, two vertical rods 19 are provided. The connection line of the two vertical rods 19 passes through the central axes of the annular top plate 17 and the annular bottom plate 18.
[0048] Nine fork-shaped placement racks 20 are evenly arranged between the annular top plate 17 and the annular bottom plate 18. One end where the apex angle of the fork-shaped placement rack 20 is located is fixedly connected to the vertical rod 19 close to the central axes of the annular top plate 17 and the annular bottom plate 18. The middle part of the fork-shaped placement rack 20 is fixedly connected to the vertical rod 19 far from the central axes of the annular top plate 17 and the annular bottom plate 18. The two tooth portions of the fork-shaped placement rack 20 face the outside of the storage turret 2, that is, away from the central axes of the annular top plate 17 and the annular bottom plate 18.
[0049] As an implementation manner, the annular bottom plate 18 further includes an inner ring. The outer ring and the inner ring of the annular bottom plate 18 are connected by a plurality of connecting rods. The top end of the central shaft 4 is fixedly connected to the inner ring of the annular bottom plate 18.
[0050] As another implementation manner, the annular bottom plate 18 further includes an inner ring. The outer ring and the inner ring of the annular bottom plate 18 are connected by a plurality of connecting rods; the annular top plate 17 further includes an inner ring. The outer ring and the inner ring of the annular top plate 17 are connected by a plurality of connecting rods. The top end of the central shaft 4 is fixedly connected to the inner ring of the annular top plate 17. The middle part of the central shaft 4 is fixedly connected to the inner ring of the annular bottom plate 18.
[0051] As an implementation manner, through holes are provided at the bottom of the box body. A first bearing is arranged in the through holes. The outer ring of the first bearing is fixedly connected to the box body, and the central shaft is fixedly connected to the inner ring of the first bearing.
[0052] The bottom end of the central shaft 4 is fixedly connected to the inner ring of the second bearing 16. The second bearing is arranged in the mounting hole opened on the upper top plate, and the outer ring of the second bearing is fixedly connected to the upper top plate. Moreover, the central axes of the second bearing and the central shaft 4 are perpendicular to the upper top plate. A gear 15 is fixedly arranged between the second bearing and the annular chassis 18 on the central shaft 14. The output shaft of the motor 12 is connected to the gear 15 through a transmission chain 13. The rotation of the output shaft of the motor drives the gear 15 to rotate, and then drives the annular chassis 18 to rotate.
[0053] The central axes of the central shaft 14, the first bearing, the second bearing, the annular chassis 18 and the gear 15 are on the same straight line.
[0054] In summary, 12 vertical rods 19 are radially arranged around the central shaft 4 on the inner ring and 12 vertical rods 19 are arranged on the outer ring, and the vertical rods 19 are distributed around the central shaft 4 at an angle of 30 degrees. The 24 vertical rod brackets 19 are fixedly connected to the central shaft 21 through the annular top plate 17 and the annular chassis 18 (or the annular chassis 18). Each vertical rod 19 is divided into 10 layers, and each layer can place 12 175 cm 2 cell culture flasks through the fork-shaped placement racks 20. Each culture flask placement position has a fixed number. The entire turret can place 120 175 cm 2 cell culture flasks, and the total culture area is 21000 cm 2 .
[0055] The entire turret is hollow and transparent up, down, left, right, front and back, which is convenient for gas flow and uniform distribution of temperature and humidity, and provides a good culture environment for the culture flasks placed on the turret.
[0056] As an implementation manner, a speed reduction mechanism is also fixed on the upper top plate and acts on the transmission chain.
[0057] Among them, the part of the central shaft 14 higher than the gear 15 is located in the box body 5, and the annular top plate 17, the annular chassis 18, the 24 vertical rods 19 and at least 108 fork-shaped placement racks 20 are also all located in the box body 5.
[0058] A humidifying device 3 is placed at the bottom in the box body 5 for holding pure water to ensure the environmental humidity in the cabin. The humidifying device 3 is two semi-circular water trays. The water trays are grooved in the middle of the straight edge for the central shaft 14 to be inserted. When the two water trays are put together, they are circular, and the central shaft 14 passes through the groove. The annular chassis 18 is located directly above the humidifying device.
[0059] Among them, the environmental monitoring sensor 4 includes a temperature sensor, a humidity sensor and a CO2 gas sensor.
[0060] Among them, the heating mechanism is an electric heating wire arranged on the side wall of the box body 5.
[0061] Among them, the number of the air-flow heat-conducting fans 10 is 4, which are fixed on the upper wall of the box body 5 and are arranged directly above the turret to make the gas in the cabin flow and ensure uniform distribution of the environmental temperature. The air-flow heat-conducting fan 10 includes an inverter and a blower.
[0062] Among them, the gas device includes an air pump, N2 and CO2 gas cylinders located outside the box body. An air inlet hole is opened at the top of the box body, and the air inlet hole is connected to the air pump for providing CO2 required for cell culture and N2 required for low-oxygen culture. The gas release amount is feedback-controlled through a gas sensor, and the air pump regulates the gas concentration in the box body.
[0063] As an implementation manner, an indicator light and a display screen are further arranged on the outer side of the box body.
[0064] As Figure 4 shown, the indicator light, the display screen (touch screen display and operation interface), the environmental monitoring sensor 4, the motor, the heating mechanism, the air-flow heat-conducting fan 10 and the gas device are all connected to a host computer (PLC controller). An intelligent environment control algorithm is configured in the host computer. The intelligent environment control algorithm includes: an environmental detection signal receiving module, a calculation module, and a control instruction output module.
[0065] The operation process of the intelligent environment control algorithm includes: the user sets target parameter values such as temperature, humidity, and gas concentration through the control panel of the display screen; the environmental monitoring sensor sends environmental parameters to the PLC controller at a certain frequency through a data transmission interface (PLC measurement module) and transmits them to the display screen; the PLC controller calculates the amount to be output for environmental adjustment through the intelligent control algorithm; the calculation result of the intelligent environment control algorithm is transmitted to the corresponding environmental adjustment device (heating mechanism, air-flow heat-conducting fan 10 and gas device) through the instruction output module for real-time regulation to maintain the constancy of the environment in the culture chamber, realizing real-time monitoring and regulation of cell growth environment parameters such as temperature, humidity, and gas in the box body, and ensuring suitable temperature, humidity, and gas concentration required for cell growth.
[0066] Among them, the indicator light shows the working state of the incubator, green for normal, red for fault, and yellow for abnormal culture state parameters. The display screen shows various parameter indicators such as temperature, humidity, and gas concentration, as well as input instructions, binds the position information of the culture bottle, and functions such as disinfection and maintenance.
[0067] As an implementation manner, a code scanning / decoding machine (scanner) is further arranged on the outer side of the box body. A cell culture management database is configured in the host computer. When storing, the information of the cell culture bottle, the storage position, and the storage time can be bound by scanning the code through the code scanning / decoding machine and inputting the position code on the display; when taking, the host computer can automatically control the rotation of the motor, and then drive the storage turret to rotate, rotating the culture bottle that meets requirements such as the culture time to the box door for convenient taking.
[0068] An intelligent storage cell culture incubator provided in this embodiment can simultaneously culture 120 cell culture flasks with a volume of 175 cm 2 , and the total culture area is as high as 21,000 cm 2 , enabling high-throughput cell culture and improving the output and efficiency of the cell culture incubator.
[0069] For the intelligent storage cell culture incubator provided in this embodiment, the rotation of the output shaft of the motor drives the gear to rotate, and then drives the annular chassis to rotate, facilitating the access of the culture flasks.
[0070] The intelligent storage cell culture incubator provided in this embodiment uses an annular top plate, an annular chassis, vertical rods, and a plurality of fork-shaped placement racks to form a turret, making the entire turret hollow and transparent in all directions (up and down, left and right, front and back), facilitating gas flow and uniform distribution of temperature and humidity, and providing a good culture environment for the culture flasks placed on the turret.
[0071] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. An intelligent storage cell culture box, characterized in that: It includes a motor, a transmission device, a first bearing, a box with a through hole at the bottom, and a storage turret arranged in the box, wherein the storage turret includes a central axis, an annular top plate, an annular bottom plate, a vertical pole and a fork-shaped placement frame; The vertical rods are evenly arranged between the annular top plate and the annular bottom plate, and a plurality of fork-shaped placement racks are evenly arranged on the vertical rods from top to bottom, and the fork-shaped placement racks are used to place cell culture bottles; The top end of the central axis is fixedly connected to the center of the annular chassis, the bottom end of the central axis passes through the through hole and extends out of the box body, the first bearing is arranged in the through hole, the outer ring of the first bearing is fixedly connected to the box body, and the central axis is fixedly connected to the inner ring of the first bearing; The output shaft of the motor is connected to the central shaft through the transmission device, so as to drive the storage turret to rotate.
2. The intelligent storage cell culture box according to claim 1, characterized in that: Also included is a support frame and a second bearing; The bottom end of the middle shaft is fixedly connected to the inner ring of the second bearing, and the outer ring of the second bearing is fixedly connected to the support frame.
3. The intelligent storage cell culture box according to claim 2, characterized in that: The connection between the transmission device and the central shaft is located between the first bearing and the second bearing.
4. The intelligent storage cell culture box according to claim 1, characterized in that: The annular top plate and the annular bottom plate both include an outer ring and a plurality of teeth evenly distributed on the outer side of the outer ring, and two vertical rods are arranged between each tooth of the annular top plate and one of the teeth of the annular bottom plate.
5. The intelligent storage cell culture box according to claim 1, characterized in that: Two semicircular water trays are placed at the bottom of the box body, and a groove adapted to the central axis is opened in the middle of the straight edge of the water tray.
6. The intelligent storage cell culture box according to claim 1, characterized in that: The side wall of the box body is provided with a heating wire.
7. The intelligent storage cell culture box according to claim 1, characterized in that: A plurality of airflow heat-conducting fans are arranged on the upper wall of the box body, and the airflow heat-conducting fans are arranged just above the storage turret.
8. The intelligent storage cell culture box according to claim 1, characterized in that: It also includes a gas cylinder containing gas and a gas pump connected to the gas cylinder; An air inlet is provided on the top of the box body, and the air inlet is connected to an air pump.
9. The intelligent storage cell culture box according to claim 1, characterized in that: It also includes a base plate, four pillars and four universal wheels; The four corners of the bottom of the box are fixedly connected to the first ends of the four pillars respectively, and the second ends of the four pillars are fixedly connected to the four corners of the top surface of the bottom plate respectively. A universal wheel is fixedly arranged at each of the four corners of the bottom surface of the bottom plate.
10. The intelligent storage cell culture box according to claim 1, characterized in that: The box body is provided with a box door, and a glass window is provided in the middle of the box door.