Sample adapter suitable for sample transparency experiment

Abstract

The utility model relates to a sample adapter suitable for a sample transparency experiment, which comprises a framework and an electrophoresis filter screen, the framework and the electrophoresis filter screen are integrally formed by injection molding, at least one sample accommodating chamber is formed by the framework and the electrophoresis filter screen, and the sample accommodating chamber is communicated with the outside through the electrophoresis filter screen. Wherein the framework and the electrophoresis filter screen are both made of plastic and do not need to be made of metal, so that the manufacturing cost is reduced. Meanwhile, the framework and the electrophoresis filter screen are directly and integrally formed through injection molding and are connected without other connecting structures, on one hand, the structural strength can be improved, and on the other hand, the manufacturing process can be simplified, and the manufacturing cost can be reduced. Meanwhile, at least one sample containing cavity is formed by the framework and the electrophoresis filter screen, the sample containing cavity is communicated with the outside through the electrophoresis filter screen, and when a plurality of sample containing cavities are manufactured, the effect of reducing the manufacturing cost is more obvious. Compared with the prior art, the purpose of remarkably reducing the manufacturing cost can be achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of sample transparency experiments, in particular to a sample adapter suitable for sample transparency experiments. Background Art

[0002] The opacity of biological tissues is caused by heterogeneous components with different optical properties, such as refractive index (RI) and light absorption rate. Most biological tissues are composed of scattering particles with a high refractive index, such as lipids, proteins, myelin sheaths, and elastic fibers, and a surrounding medium with a low refractive index, such as interstitial fluid and cytoplasm. Due to each component having a different refractive index, this heterogeneous material structure will scatter incident light, limiting the optical imaging depth. In addition, the light absorption of endogenous pigments such as heme, riboflavin, melanin, and lipofuscin will also attenuate the light propagation. Therefore, changing the optical properties of tissue heterogeneous components and reducing light scattering and light absorption are the keys to increasing tissue imaging depth. With the development and popularization of optical microscopes, as well as the progress of data acquisition and storage technologies, tissue clearing techniques have made whole-organ and even whole-body imaging possible. In recent years, researchers have developed a series of tissue clearing techniques, and all clearing techniques are dedicated to balancing tissue refractive indices to reduce the inhomogeneity of light scattering. Tissue clearing techniques can make biological tissues "transparent" to light. Combined with fluorescence imaging and three-dimensional reconstruction techniques, the contents and structures of tissues can be observed and analyzed three-dimensionally at the cellular level, having a huge impact in the field of life sciences. Especially in the field of neuroscience, studying specific protein expression, localization, ultrastructure, and neural circuit tracing of brain tissues or cells has attracted the attention and favor of many scientific researchers.

[0003] Current clearing methods can be divided into three categories: organic solvent clearing techniques, hydrophilic reagent clearing techniques, and hydrogel-embedded tissue clearing techniques. X-Clarity is one of the most commonly used hydrophilic reagent clearing techniques. X-Clarity is a tissue clearing processing system based on electrophoresis principles, which realizes the clearing of brain tissues or other tissues such as the pancreas to understand the structural / functional relationships between cells, circulation pathways, and organs, so as to construct 3D anatomical maps and phenotypic maps.

[0004] Electrophoretic tissue clearing is the most crucial step, which requires fixing the sample in an adapter, immersing it in a clearing buffer, and performing electrophoretic degreasing using an X-Clarity clearing system. Among them, in order to enable the sample adapter to meet the conditions for use in the electrophoresis solution and to make the electrophoretic degreasing faster, an electrophoresis filter screen is often loaded on the sample adapter through a connection structure, and the electrophoresis filter screen has channels with micron-level apertures for the electrophoresis solution to pass through. Therefore, in the prior art, it is necessary to manufacture the sample adapter and the electrophoresis filter screen sequentially and then assemble the two, which makes the manufacturing process of the sample adapter relatively complex and results in a high manufacturing cost. Summary of the Invention

[0005] In order to solve the defect of high manufacturing cost of the sample adapter in the prior art, the present invention proposes a sample adapter suitable for sample clearing experiments.

[0006] The technical solution adopted by the present invention is a sample adapter suitable for sample clearing experiments, which includes a skeleton and an electrophoresis filter screen. The skeleton and the electrophoresis filter screen are integrally injection-molded. The skeleton and the electrophoresis filter screen form at least one sample accommodation chamber, and the electrophoresis solution can flow through the electrophoresis filter screen inside and outside the sample accommodation chamber.

[0007] Preferably, the skeleton and the electrophoresis filter screen form at least two sample accommodation chambers, and any two adjacent sample accommodation chambers are connected through the electrophoresis filter screen.

[0008] Preferably, there are four sample accommodation chambers, and the sample accommodation chambers are arranged in a 2×2 pattern.

[0009] Preferably, there are six sample accommodation chambers, and the sample accommodation chambers are arranged in a 3×2 pattern.

[0010] Preferably, the tops of multiple sample accommodation chambers all have openings, and the skeleton is connected with a protective cover for opening or closing the openings.

[0011] Preferably, the protective cover has a through hole, and the top of the sample accommodation chamber is connected to the outside through the through hole.

[0012] Preferably, one of the skeleton and the protective cover has a buckle, and the other has a buckle mating part. When the buckle and the buckle mating part cooperate with each other, the protective cover closes the opening.

[0013] Preferably, the shape of the sample accommodation chamber is a hexahedron, and the skeleton and the electrophoresis filter screen respectively constitute the edges and faces of the sample accommodation chamber.

[0014] Preferably, the sample adapter is made of polypropylene, polycarbonate, polyethylene, polyamide, polystyrene, polyfluoride or cellulose acetate butyrate.

[0015] Preferably, the pore size range of the electrophoresis filter screen is 40 to 70 micrometers.

[0016] Preferably, the sample accommodation chamber is provided with a flexible adaptation part. The flexible adaptation part contacts the sample, and the shape of the flexible adaptation part changes with the position of the sample in the sample accommodation chamber.

[0017] Compared with the prior art, the present utility model has the following beneficial effects:

[0018] The present application discloses a sample adapter applicable to sample clearing experiments, including a skeleton and an electrophoresis filter screen. Both the skeleton and the electrophoresis filter screen are made of plastic and do not need to be manufactured using metal, thereby reducing the manufacturing cost. At the same time, the skeleton and the electrophoresis filter screen are directly integrally formed by injection molding, and no other connecting structures are required to connect them. On the one hand, the structural strength can be improved, and on the other hand, the manufacturing process can be simplified to reduce the manufacturing cost. At the same time, the skeleton and the electrophoresis filter screen form at least one sample accommodation chamber, and the sample accommodation chamber communicates with the outside through the electrophoresis filter screen. When multiple sample accommodation chambers are manufactured, the effect of reducing the manufacturing cost is more obvious.

[0019] Compared with the prior art, a sample adapter disclosed in the present application applicable to sample clearing experiments can achieve the purpose of significantly reducing the manufacturing cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present utility model will be described in detail below in conjunction with embodiments and drawings, where:

[0021] Figure 1 shows a schematic structural diagram of a sample adapter applicable to sample clearing experiments provided by an embodiment of the present utility model;

[0022] Figure 2 shows according to Figure 1 a bottom view of a sample adapter applicable to sample clearing experiments provided;

[0023] Figure 3 shows according to Figure 1 a schematic structural diagram of a protective cover of a sample adapter applicable to sample clearing experiments provided;

[0024] Figure 4 shows according to Figure 1 a schematic structural diagram of a sample adapter applicable to sample clearing experiments provided with a protective cover installed.

[0025] Label description:

[0026] 1. Skeleton; 2. Electrophoresis filter screen; 3. Sample accommodation chamber; 4. Protective cover; 5. Through hole; 6. Snap; 7. Snap fit part. Detailed implementation mode

[0027] To make the objectives, technical solutions and advantages of the present utility model clearer, the following will further describe in detail the implementation modes of the present utility model in conjunction with the accompanying drawings. Examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar components or components with the same or similar functions from beginning to end. The embodiments described below by referring to the drawings are exemplary and are only used to explain the present utility model, and cannot be construed as a limitation to the present utility model.

[0028] The present utility model discloses a sample adapter applicable to sample transparency experiments. Please refer to Figures 1 to 4 , which includes a skeleton 1 and an electrophoresis filter mesh 2. The skeleton 1 and the electrophoresis filter mesh 2 are integrally formed by injection molding. At least one sample accommodation chamber 3 is formed between the skeleton 1 and the electrophoresis filter mesh 2, and the sample accommodation chamber 3 communicates with the outside through the electrophoresis filter mesh 2.

[0029] Both the skeleton 1 and the electrophoresis filter mesh 2 are made of plastic and do not need to be manufactured using metal, thereby reducing the manufacturing cost. At the same time, the skeleton 1 and the electrophoresis filter mesh 2 are directly integrally formed by injection molding, and no other connection structures are required to connect the two. On the one hand, the structural strength can be improved, and on the other hand, the manufacturing process can be simplified to reduce the manufacturing cost. At the same time, at least one sample accommodation chamber 3 is formed between the skeleton 1 and the electrophoresis filter mesh 2, and the sample accommodation chamber 3 communicates with the outside through the electrophoresis filter mesh 2. When multiple sample accommodation chambers 3 are manufactured, the effect of reducing the manufacturing cost is more obvious. Compared with the prior art, a sample adapter disclosed in the present application applicable to sample transparency experiments can achieve the purpose of reducing the manufacturing cost.

[0030] Specifically, the skeleton 1 and the electrophoresis filter mesh 2 are integrally formed by injection molding. At the same time, there is no bonding structure between the two and no other connection structures are used for connection, and the entire structure is completed in one operation. Therefore, on the basis of achieving the purpose of reducing the manufacturing cost, the sample adapter manufactured by the injection molding integration method can improve the integrity and strength of the structure, can better withstand various stresses and impacts, and is not easily detached and damaged.

[0031] It should be noted that when there are multiple sample accommodation chambers 3, in the prior art, since more electrophoresis filter meshes 2 need to be assembled on the skeleton 1, the manufacturing process is more complex. However, in the present application, through the injection molding integration process, manufacturing a sample adapter with multiple sample accommodation chambers 3 will not result in an increase in the manufacturing process, thereby further simplifying the manufacturing process of the sample adapter.

[0032] Among them, the skeleton 1 is used to support the electrophoresis filter mesh 2, so that the electrophoresis filter mesh 2 is not easily deformed. The size occupied by the skeleton 1 should be much smaller than that of the electrophoresis filter mesh 2, allowing the electrophoresis solution to enter the sample accommodation chamber 3 through the electrophoresis filter mesh 2 at more positions.

[0033] In some embodiments, the skeleton 1 and the electrophoresis filter mesh 2 form at least two sample accommodation chambers 3, and any two adjacent sample accommodation chambers 3 communicate with each other through the electrophoresis filter mesh 2.

[0034] Specifically, the skeleton 1 and the electrophoresis filter mesh 2 can form at least two sample accommodation chambers 3, and communicate and separate through the electrophoresis filter mesh 2 between any two adjacent sample accommodation chambers 3, so that the electrophoresis solutions in any two adjacent sample accommodation chambers 3 can also flow through each other, enabling the electrophoresis solution and the sample to be in uniform contact to improve the degreasing effect.

[0035] In some specific embodiments, there are four sample accommodation chambers 3, and the sample accommodation chambers are arranged in a 2×2 pattern.

[0036] It should be noted that there are four sample accommodation chambers 3 and they are arranged in a 2×2 pattern. Such a setting can make the area of each sample accommodation chamber 3 communicating with the external electrophoresis solution through the electrophoresis filter mesh 2 the same, so that the flow rate of the electrophoresis solution in each sample accommodation chamber 3 is approximately the same, enabling the samples placed in different sample accommodation chambers 3 to obtain the same degreasing effect and avoiding affecting the results of multiple control experiments due to variables caused by degreasing. For example, when the shape of the sample accommodation chamber 3 is a hexahedron, when the sample adapter is partially immersed in the electrophoresis solution, each sample accommodation chamber 3 only has the bottom surface and two side surfaces communicating with the outside, thus obtaining a uniform degreasing effect.

[0037] In other embodiments, the arrangement of the sample accommodation chambers 3 can also be in quantities such as 3×2, 4×4, etc., which is convenient for the operator to select according to the application scenario.

[0038] In some specific embodiments, please refer to Figure 3 and Figure 4 , the tops of multiple sample accommodation chambers 3 all have openings, and the skeleton 1 is connected with a protective cover 4 for opening or closing the openings.

[0039] Among them, the top of the accommodation chamber has an opening, and the opening is opened or closed by the protective cover 4, so as to prevent the sample from running out from the top of the accommodation chamber.

[0040] In other embodiments, the protective cover 4, the skeleton 1, and the electrophoresis filter 2 are integrally injection-molded. This further simplifies the manufacturing process of the sample adapter and reduces the manufacturing cost. Specifically, after the protective cover 4 is integrally injection-molded, it is connected to a skeleton 1. The opening is closed or fixed by bending the protective cover 4 at the crease where it is connected to the skeleton 1. In addition, a lock structure that can be loosened or locked can also be integrally formed, making the closing effect of the protective cover 4 on the opening better. Preferably, the protective cover 4 and the electrophoresis filter 2 have the same structure, that is, the protective cover 4 also has channels with nano- or micron-sized pores for the electrophoresis solution to pass through, allowing the electrophoresis solution to flow fully through the entire sample adapter.

[0041] In some more specific embodiments, the protective cover 4 has a through-hole 5, and the top of the sample accommodation chamber 3 communicates with the outside through the through-hole 5.

[0042] Specifically, the protective cover 4 has a through-hole 5, and the through-hole 5 enables the electrophoresis solution to flow fully through the entire sample adapter.

[0043] In some more specific embodiments, one of the skeleton 1 and the protective cover 4 has a buckle 6, and the other has a buckle 6 mating part. When the buckle 6 and the buckle 6 mating part cooperate with each other, the protective cover 4 closes the opening.

[0044] To make the closing effect of the protective cover 4 better, a buckle 6 and a buckle 6 mating part are provided to prevent the protective cover 4 from falling off.

[0045] In some embodiments, the shape of the sample accommodation chamber 3 is a hexahedron, and the skeleton 1 and the electrophoresis filter 2 respectively form the edges and faces of the sample accommodation chamber 3.

[0046] It should be noted that the skeleton 1 and the electrophoresis filter 2 respectively form the edges and faces of the sample accommodation chamber 3, forming a hexahedron sample accommodation chamber 3. This setting can make the structure of the sample adapter stronger, the overall structure more stable, and at the same time, more areas can be provided for setting the electrophoresis filter 2 to improve the electrophoresis effect.

[0047] In some embodiments, the sample adapter is made of polypropylene, polycarbonate, polyethylene, polyamide, polystyrene, polyfluoride, or cellulose acetate butyrate.

[0048] In some embodiments, the pore size range of the electrophoresis filter 2 is from 40 microns to 70 microns.

[0049] Preferably, the pore size of the electrophoresis filter 2 is about 45 microns.

[0050] In the description of this specification, terms such as "Example 1", "this example", "in one example", etc. mean that the specific features, structures, materials or characteristics described in connection with the example or illustration are included in at least one example or illustration of the utility model or the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same example or illustration; moreover, the specific features, structures, materials or characteristics described may be combined in any one or more examples or illustrations in an appropriate manner.

[0051] In the description of this specification, terms such as "connection", "installation", "fixation", "setting", "having", etc. are all understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0052] In the description of this specification, relative terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variation thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements but also other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "including one..." does not exclude the existence of additional identical elements in the process, method, article or device including the said element.

[0053] The above description of the embodiments is for the convenience of those of ordinary skill in the art to understand and apply the technology of this case. Obviously, those who are familiar with the technology in this field can easily make various modifications to these examples and apply the general principles described here to other embodiments without creative labor. Therefore, this case is not limited to the above embodiments. For the following several types of modifications, they should all be within the protection scope of this case: ① A new technical solution implemented based on the technical solution of the present utility model and combined with the existing common knowledge, and the technical effect produced by this new technical solution does not exceed the technical effect of the present utility model; ② An equivalent replacement of some features of the technical solution of the present utility model using well-known technologies, and the technical effect produced is the same as the technical effect of the present utility model; ③ Expansion based on the technical solution of the present utility model, and the substantial content of the expanded technical solution does not exceed the technical solution of the present utility model; ④ An equivalent transformation made using the content of the specification and drawings of the present utility model, directly or indirectly applied in other related technical fields.

Claims

1. A sample adapter applicable to sample clearing experiments, characterized in that, It includes a framework and an electrophoresis filter screen. The framework and the electrophoresis filter screen are integrally injection-molded. At least one sample accommodating chamber is formed between the framework and the electrophoresis filter screen, and the sample accommodating chamber communicates with the outside through the electrophoresis filter screen.

2. The sample adapter applicable to the sample transparency experiment according to claim 1, characterized in that, At least two sample accommodating chambers are formed between the framework and the electrophoresis filter screen, and any two adjacent sample accommodating chambers communicate with each other through the electrophoresis filter screen.

3. The sample adapter applicable to the sample transparency experiment according to claim 2, wherein There are four sample accommodating chambers, and the sample accommodating chambers are arranged in a 2×2 pattern.

4. A sample adapter applicable to a sample transparency experiment according to claim 2, characterized in that, Openings are provided at the tops of multiple sample accommodating chambers, and a protective cover for opening or closing the openings is connected to the framework.

5. The sample adapter applicable to the sample transparency experiment according to claim 4, characterized in that, The protective cover has a through hole, and the top of the sample accommodating chamber communicates with the outside through the through hole.

6. The sample adapter applicable to the sample transparency experiment according to claim 4, characterized in that One of the framework and the protective cover has a buckle, and the other has a buckle mating part. When the buckle and the buckle mating part cooperate with each other, the protective cover closes the opening.

7. A sample adapter applicable to a sample transparency experiment according to claim 1, characterized in that, The shape of the sample accommodating chamber is a hexahedron, and the framework and the electrophoresis filter screen respectively constitute the edges and faces of the sample accommodating chamber.

8. The sample adapter applicable to the sample transparency experiment according to claim 1, characterized in that, The sample adapter is made of polypropylene, polycarbonate, polyethylene, polyamide, polystyrene, polyfluoride or cellulose acetate butyrate.

9. A sample adapter applicable to a sample transparency experiment according to claim 1, characterized in that, The pore size range of the electrophoresis filter screen is from 40 microns to 70 microns.

10. A sample adapter applicable to a sample transparency experiment according to claim 1, characterized in that, A flexible adapter part is provided in the sample accommodating chamber. The flexible adapter part contacts the sample and the shape of the flexible adapter part changes with the position of the sample in the sample accommodating chamber.

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