Cellulase production device

Abstract

The utility model relates to the technical field of cellulase production, in particular to a cellulase production device which comprises a fermentation tank, a refrigeration tank and an air compressor. Two air pipes, namely a first air pipe and a second air pipe, are led out from an air outlet pipe of the air compressor, and the first air pipe is connected with an air inlet pipe on the fermentation tank; the second air pipe is connected with an air inlet in the refrigeration tank, a conveying pipe is arranged in the refrigeration tank, the lower end of the conveying pipe extends to the bottom of the refrigeration tank, and the conveying pipe is connected with a strain adding pipe on the fermentation tank. Intermittent aeration in the fermentation tank can be realized through the air machine, addition of a strain solution can be realized, the cost of strain solution conveying equipment is saved, death of strains caused by strong mechanical force in a pump conveying process can be effectively avoided, the activity of the strains is effectively ensured, and the yield of the strains is improved. The method plays a positive role in efficient production of cellulase.

Description

Technical Field

[0001] The utility model relates to the technical field of cellulase production, and particularly relates to a cellulase production device. Background Art

[0002] Cellulase acts on cellulose and products derived from cellulose, and is a general term for a group of enzymes that degrade cellulose to produce glucose. It is not a monomeric enzyme, but a multi-component enzyme system that acts synergistically, and is a composite enzyme, mainly composed of exo-β-glucanase, endo-β-glucanase, and β-glucosidase, etc., and also has a very high activity of xylanase; cellulase is of great significance in converting insoluble cellulose into glucose and in breaking the cell wall in fruit and vegetable juices to improve the juice yield.

[0003] Currently, cellulase is generally prepared by fermentation. Among the microbial strains, filamentous fungi are more common. Among them, the strains with stronger enzyme activity are Trichoderma, Aspergillus, and Penicillium. In particular, Trichoderma is currently a better cellulase-producing bacterium; the fermentation method uses crop straws such as corn as the main raw material. During the process, the fermentation raw materials are added to the fermentation tank, and then the strains are added at an appropriate temperature and pH, and intermittent aeration is carried out during the fermentation process to complete the process of fermenting and producing cellulase.

[0004] In actual production, the prepared strain solution is generally stored in a refrigeration tank for preservation, and then pumped to the fermentation tank during use. During the pumping process, due to the strong centrifugal force and shear force of the mechanical equipment, some of the bacteria in the strain solution will die, thus affecting the fermentation production efficiency of cellulase; therefore, a cellulase production device that can avoid the death of bacteria in the strain solution during the transportation process and thus effectively improve the fermentation production efficiency of cellulase is a problem that needs to be solved currently. Summary of the Utility Model

[0005] The purpose of the utility model is to aim at the above-mentioned deficiencies of the prior art and provide a cellulase production device to effectively ensure the activity of the strains and thus effectively ensure the fermentation production efficiency of cellulase.

[0006] To achieve the above purpose, the utility model adopts the following technical scheme: a cellulase production device, including a fermentation tank, a refrigeration tank, and an air compressor. The air outlet pipe of the air compressor is divided into two air pipes, which are the first air pipe and the second air pipe respectively. The first air pipe is connected to the air inlet pipe on the fermentation tank, and the air inlet pipe is connected to the air diffuser pipe; the second air pipe is connected to the air inlet on the refrigeration tank. A delivery pipe is arranged in the refrigeration tank, and the lower end of the delivery pipe extends to the bottom of the refrigeration tank. The delivery pipe is connected to the strain addition port on the fermentation tank.

[0007] Further, an air filter is installed on the air inlet pipe of the air compressor.

[0008] Further, the aeration pipe is arranged at the bottom of the fermentation tank and is arranged in a circular shape.

[0009] Further, a stirring device is provided in the fermentation tank. The stirring device includes a motor speed reducer, which is installed on the tank body of the fermentation tank, and the motor speed reducer is connected to the stirring paddle inside the tank body.

[0010] Further, a material inlet, a water inlet, and a pH adjustment port are also provided on the fermentation tank. The water inlet is connected to a water inlet pipe, the pH adjustment port is connected to a pH adjustment pipe, and a peristaltic pump is installed on the pH adjustment pipe. A discharge port is provided at the bottom of the fermentation tank, and the discharge port is connected to a discharge pipe.

[0011] Further, a jacket is provided outside the tank body of the fermentation tank. A medium inlet is provided at the bottom of the jacket, and a medium outlet is provided above the jacket.

[0012] Further, an instrument installation port is also provided on the tank body of the fermentation tank. The instrument installation port includes a pH electrode installation port and a temperature probe installation port. A pH electrode is installed through the pH electrode installation port, and a temperature probe is installed through the temperature probe installation port.

[0013] The beneficial effects of the present utility model are as follows: Intermittent aeration in the fermentation tank can be achieved through the air machine, and the addition of the strain solution can also be realized. While saving the cost of the strain solution transportation equipment, it can also effectively avoid the death of the strain caused by strong mechanical force during the pump transportation process, effectively ensuring the activity of the strain, and playing a positive role in the efficient production of cellulase. Description of the Drawings

[0014] Figure 1 is a schematic diagram of the overall structure of the present utility model;

[0015] Figure 2 is a top view schematic diagram of the fermentation tank of the present utility model;

[0016] Figure 3 is the present utility model Figure 1 The top view schematic diagram of the A-A plane in.

[0017] The names corresponding to the marks in the figure:

[0018] 1. Fermentation tank; 11. Stirring device; 111. Stirring paddle; 12. Feed inlet; 13. Water inlet; 131. Water inlet pipe; 14. pH adjustment port; 141. pH adjustment pipe; 15. Air inlet pipe; 151. Aeration pipe; 16. Discharge port; 161. Discharge pipe; 17. Jacket; 171. Medium inlet; 172. Medium outlet; 18. Bacteria addition port; 19. Instrument installation port; 191. pH electrode installation port; 192. Temperature probe installation port; 2. Refrigeration tank; 21. Air inlet; 22. Delivery pipe; 3. Air compressor; 31. Air inlet pipe; 311. Air filter; 32. Air outlet pipe; 321. First air duct; 322. Second air duct. DETAILED DESCRIPTION

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the utility model in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the utility model.

[0020] Embodiments of the present utility model:

[0021] like Figures 1-3 As shown, in the cellulase production device of this embodiment, a fermentation tank 1 is provided, a stirring device 11 is provided in the fermentation tank 1, a motor reducer is provided in the stirring device 11, the motor reducer is installed on the tank body, the motor reducer is connected to the stirring paddle 111 inside the tank body, a support frame is provided below the stirring paddle 111, the stirring paddle 111 is connected to the support frame through a bearing, so as to ensure the stability of the stirring paddle 111 during use.

[0022] A feed port 12, a water inlet 13 and a pH adjustment port 14 are provided on the tank body of the fermentation tank 1. The feed port 12 is used for adding fermentation material raw material powder, such as straw powder, etc.; the water inlet 13 is connected to a water inlet pipe 131 for adding water, and an electric valve, a flow display transmitter and a water pump are installed on the water inlet pipe 131. The water inlet pipe 131 is connected to a pure water storage tank for conveying pure water; the pH adjustment port 14 is connected to a pH adjustment tube 141, and the pH adjustment tube 141 is connected to a pH adjuster storage tank, and a peristaltic pump is installed on the pH adjustment tube 141 for adjusting the pH in the fermentation tank 1.

[0023] An air inlet pipe 31 is also provided on the tank body of the fermentation tank 1. The air inlet pipe 31 is connected to the aeration pipe 151 in the tank body. Intermittent aeration is carried out in the tank body through the aeration pipe 151. A discharge port 16 is provided at the bottom of the tank body of the fermentation tank 1. The discharge port 16 is connected to a discharge pipe 161. An electric valve and a transfer pump are provided on the discharge pipe 161 for discharging the materials after fermentation is completed.

[0024] A jacket 17 is also provided on the tank body of the fermentation tank 1. A medium inlet 171 is provided at the bottom of the jacket 17, and a medium outlet 172 is provided above the jacket 17. Circulating medium is connected through the jacket 17 for controlling the temperature during the fermentation process. An electric valve is provided at the medium inlet 171, and a flow display transmitter instrument is provided at the medium outlet 172. An instrument installation port 19 is also provided on the tank body of the fermentation tank 1. The instrument installation port 19 includes a pH electrode installation port 191 and a temperature probe installation port 192. A pH electrode is installed through the pH electrode installation port 191. The pH electrode cooperates with a peristaltic pump for controlling the pH in the tank body. The temperature probe cooperates with the flow rate of the circulating medium in the jacket 17 to control the temperature in the tank body.

[0025] An air compressor 3 is provided and connected to the air inlet pipe 15 on the tank body of the fermentation tank 1. An air filter 311 is provided on the air inlet pipe 31 of the air compressor 3. The air outlet pipe 32 of the air compressor 3 is divided into two air pipes through a tee, namely a first air pipe 321 and a second air pipe 322. The first air pipe 321 is connected to the air inlet pipe 15 on the fermentation tank 1, and an electric valve and a flow indicating transmitter instrument are provided on the first air pipe 321. The second air pipe 322 is connected to the air inlet 21 on the refrigeration tank 2, and an electric valve and a pressure indicating transmitter instrument are provided on the second air pipe 322. A delivery pipe 22 is provided in the refrigeration tank 2. The lower end of the delivery pipe 22 extends to the bottom of the refrigeration tank 2. The delivery pipe 22 is connected to the strain inoculation port 18 on the fermentation tank 1, and an electric valve and a flow indicating transmitter instrument are also provided on the delivery pipe 22.

[0026] In this embodiment, the above-mentioned instruments, electric valves, and pumps are all connected to the on-site PLC.

[0027] The principle of the present utility model is:

[0028] When the present utility model is in use, a certain amount of water is conveyed into the fermentation tank 1 through the water inlet 13 during the process. Then, raw material powder, such as straw powder, is added to the tank body and stirring is started. At this time, it is adjusted to an appropriate pH through the pH adjustment pipe 141, such as a pH between 4 and 5, and the temperature in the tank body is regulated to a suitable temperature through the circulating medium, such as 25 to 30 °C. At this time, the strain can be added.

[0029] The prepared bacterial strain solution is stored in the refrigeration tank 2 and preserved at 2°C. The preparation of the bacterial strain solution and the principle of the refrigeration tank 2 are mature existing technologies and will not be elaborated here. After the above operations are completed, the addition of the bacterial strain can be started. During this process, the first air duct 321 is closed and the second air duct 322 is started. Then, under the action of pressure, the bacterial strain solution in the refrigeration tank 2 can be pressed into the fermentation tank 1. After the addition of the bacterial strain is completed, the fermentation process is started. After the fermentation is completed, the fermented liquid is discharged from the bottom discharge pipe 161 and can be made into cellulase after filtration and concentration.

[0030] During the fermentation process, the tank body needs to be aerated at a certain frequency. During this process, the first air duct 321 is opened and the second air duct 322 is kept closed. Then, the aeration process in the tank body can be realized. The utility model can realize the transportation of the bacterial strain solution and intermittent aeration during the fermentation process through an air compressor 3, saving the transportation equipment required during the addition of the bacterial strain, effectively reducing the equipment cost during the production process. At the same time, adopting the pneumatic transportation method can effectively avoid the death of the bacterial strain under the action of strong mechanical force, ensure the activity of the bacterial strain, and thus play a positive role in the efficient production of cellulase.

Claims

1. An apparatus for producing cellulase, comprising a fermentation tank (1), a refrigeration tank (2) and an air compressor (3), characterized in that: The air outlet pipe (32) of the air compressor (3) is divided into two air pipes, namely the first air pipe (321) and the second air pipe (322). The first air pipe (321) is connected to the air inlet pipe (15) on the fermentation tank (1), and the air inlet pipe (15) is connected to the air diffuser pipe (151). The second air pipe (322) is connected to the air inlet (21) on the refrigeration tank (2). A delivery pipe (22) is arranged in the refrigeration tank (2), and the lower end of the delivery pipe (22) extends to the bottom of the refrigeration tank (2). The delivery pipe (22) is connected to the strainer addition port (18) on the fermentation tank (1).

2. The production device of a cellulase according to claim 1, wherein: An air filter (311) is installed on the air inlet pipe (31) of the air compressor (3).

3. The production device of a cellulase according to claim 1, characterized in that: The air diffuser pipe (151) is arranged at the bottom of the fermentation tank (1) and is arranged in a circular shape.

4. The production device of a cellulase according to claim 1, characterized in that: A stirring device (11) is arranged in the fermentation tank (1). The stirring device (11) includes a motor reducer, and the motor reducer is installed on the tank body of the fermentation tank (1). The motor reducer is connected to the stirring paddle (111) inside the tank body.

5. The production device of a cellulase according to claim 1, characterized in that: The fermentation tank (1) is also provided with a material inlet, a water inlet (13), and a pH adjustment port (14). The water inlet (13) is connected to a water inlet pipe (131), and the pH adjustment port (14) is connected to a pH adjustment pipe (141). A peristaltic pump is installed on the pH adjustment pipe (141). A discharge port (16) is arranged at the bottom of the fermentation tank (1), and the discharge port (16) is connected to a discharge pipe (161).

6. The production device of a cellulase according to claim 1, characterized in that: A jacket (17) is arranged outside the tank body of the fermentation tank (1). A medium inlet (171) is arranged at the bottom of the jacket (17), and a medium outlet (172) is arranged above the jacket (17).

7. A production device for cellulase according to any one of claims 5-6, characterized in that: An instrument installation port (19) is also arranged on the tank body of the fermentation tank (1). The instrument installation port (19) includes a pH electrode installation port (191) and a temperature probe installation port (192). A pH electrode is installed through the pH electrode installation port (191), and a temperature probe is installed through the temperature probe installation port (192).

Images