Constant-temperature PRP centrifugal assembly

Abstract

The utility model discloses a constant-temperature PRP centrifugal assembly which comprises a constant-temperature cylinder, the outer diameter of the constant-temperature cylinder is equal to the inner diameter of a centrifugal cylinder, the constant-temperature cylinder is placed in the centrifugal cylinder of a PRP centrifugal device during use, and the constant-temperature cylinder is driven by the PRP centrifugal device to rotate to complete centrifugal operation; a needle cylinder centrifugal bin and a power source bin which are independently arranged are arranged in the constant-temperature cylinder, the top of the needle cylinder centrifugal bin and the top of the power source bin are open, the needle cylinder centrifugal bin is used for containing an injector containing blood, and the power source bin is used for containing a power source. An electric heating wire is wound on the outer wall of the needle cylinder centrifugal bin, is electrically connected with a power supply and is used for heating the needle cylinder centrifugal bin in the centrifugal process; and a switch for controlling the on-off of the heating wire and a heating gear adjusting button are arranged on the power supply. The constant-temperature cylinder is directly mounted on a centrifugal tank of a 250ml centrifugal machine through combination; the heat preservation bin is powered by the USB mobile power source, a centrifugal machine circuit does not need to be changed, an existing centrifugal machine structure does not need to be adjusted, and universality of the centrifugal machine is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of medical devices, and specifically belongs to a constant-temperature PRP centrifugation assembly. Background Art

[0002] PRP, that is, platelet-rich plasma, is a blood component widely used in regenerative medicine and pain management. Although PRP is autologous blood, allergic reactions generally do not occur. The centrifugation extraction of PRP is affected by temperature, and low temperature will result in unqualified situations such as a low multiple of the prepared PRP. Content of the Utility Model

[0003] To solve the problem of unqualified situations such as a low multiple of PRP during low-temperature centrifugation for preparing PRP, the utility model provides a constant-temperature PRP centrifugation assembly, which directly uses a sterile syringe to prepare PRP by simulating the low-heat temperature environment of the human body.

[0004] For this purpose, the utility model adopts the following technical solutions:

[0005] A constant-temperature PRP centrifugation assembly includes a constant-temperature cylinder. The outer diameter of the constant-temperature cylinder is equal to the inner diameter of the centrifugation cylinder. During use, the constant-temperature cylinder is placed inside the centrifugation cylinder of the PRP centrifugation device, and the centrifugation operation is completed by driving the constant-temperature cylinder to rotate through the PRP centrifugation device;

[0006] The constant-temperature cylinder includes an independent and arranged syringe centrifugation chamber and a power supply chamber. The tops of the syringe centrifugation chamber and the power supply chamber are open. The syringe centrifugation chamber is used to place the syringe containing blood, and the power supply chamber is used to place the power supply; an electric heating wire is wound around the outer wall of the syringe centrifugation chamber, and the electric heating wire is electrically connected to the power supply. The electric heating wire is used to heat the syringe centrifugation chamber during centrifugation; the power supply is provided with a switch for controlling the on-off of the electric heating wire and a heating gear adjustment button.

[0007] Further, the heating gear adjustment button adopts button adjustment or knob adjustment.

[0008] Further, a cylindrical plug is inserted at the top opening of the syringe centrifugation chamber. The bottom of the plug abuts against the syringe, and the syringe is fixed inside the syringe centrifugation chamber through the plug to prevent the syringe from shaking during centrifugation.

[0009] Further, the side of the power supply chamber is provided with an opening for the power cord to pass through and connect to the electric heating wire.

[0010] Further, a spiral wire groove is prefabricated on the outer wall of the syringe centrifugation chamber, and the electric heating wire is coiled along the wire groove; an adhesive tape is pasted on the outer surface of the syringe centrifugation chamber to fix the electric heating wire.

[0011] The beneficial effects of the utility model are as follows:

[0012] 1. The constant temperature cylinder of the present utility model is directly installed on the centrifugal tank of a 250 ml centrifuge through combination; the heat preservation bin is powered by a USB mobile power supply, without the need to change the centrifuge circuit or adjust the existing centrifuge structure, thus improving the versatility of the present utility model;

[0013] 2. Simulate the low-temperature constant centrifugation of the human body, prevent the loss of PRP due to wall attachment during centrifugation, and ensure the maximization of the PRP multiple;

[0014] 3. Directly use a sterile syringe for centrifugation, avoiding the possible contamination caused during the process of injecting blood into the test tube; the syringe is a type-three original instrument that has been used clinically for many years, ensuring the sterility of the PRP preparation process and meeting the treatment requirements. Description of the Drawings

[0015] Figure 1 is a schematic structural diagram of the constant temperature cylinder of the present utility model;

[0016] Figure 2 is a schematic diagram of the constant temperature cylinder of the present utility model placed in the centrifugal cylinder;

[0017] Figure 3 is a schematic diagram of the constant temperature cylinder of the present utility model placed in the centrifugal device;

[0018] In the figure: 1 - centrifugal cylinder, 2 - constant temperature cylinder, 21 - syringe centrifugation bin, 22 - power supply bin, 221 - opening, 3 - wire, 4 - heating wire, 5 - syringe, 6 - plug, 7 - power supply, 8 - centrifugal device. Detailed Embodiment

[0019] The following further illustrates the present utility model in conjunction with the drawings and specific embodiments:

[0020] As Figure 1 and 2 shown, a constant temperature PRP centrifugation assembly includes a constant temperature cylinder 2 made of a polymer material. The constant temperature cylinder 2 is manufactured by 3D printing or injection molding. The outer diameter of the constant temperature cylinder 2 is equal to the inner diameter of the centrifugal cylinder 1. During use, the constant temperature cylinder 2 is placed in the centrifugal cylinder 1 of the PRP centrifugal device 8, and the centrifugal operation is completed by driving the constant temperature cylinder 2 to rotate through the PRP centrifugal device 8.

[0021] As Figure 1As shown in the figure, the constant temperature cylinder 2 includes an independently arranged syringe centrifuge chamber 21 and a power supply chamber 22. The syringe centrifuge chamber 21 and the power supply chamber 22 are separately manufactured. They are independent structures and the joint surface is an arc surface. After combining the two chambers, the constant temperature cylinder 2 is formed. The tops of the syringe centrifuge chamber 21 and the power supply chamber 22 are open. The syringe centrifuge chamber 21 is used to place the syringe 5 containing blood, and the power supply chamber 22 is used to place the power supply 7. An electric heating wire 4 is wound around the outer wall of the syringe centrifuge chamber 21. To improve the fixing strength of the electric heating wire 4, spiral wire grooves are prefabricated on the outer wall of the syringe centrifuge chamber 21, and the electric heating wire 4 is arranged along the wire grooves; an adhesive tape is pasted on the outer surface of the syringe centrifuge chamber 21 to fix the electric heating wire 4 through the adhesive tape. The electric heating wire 4 and the power supply 7 are electrically connected through a wire 3. The electric heating wire 4 is used to heat the syringe centrifuge chamber 21 during centrifugation; the power supply 7 is provided with a switch for controlling the on-off of the electric heating wire 4 and a heating gear adjustment button, and the heating gear adjustment button is adjusted by a key or a knob. To facilitate the arrangement of the wire 3, an opening 221 is provided on the side of the power supply chamber 22, and the wire 3 passes through the opening 221 and is connected to the electric heating wire 4.

[0022] A cylindrical plug 6 is also inserted at the opening 221 at the top of the syringe centrifuge chamber 21. The bottom of the plug 6 abuts against the syringe 5, and the syringe 5 is fixed in the syringe centrifuge chamber 21 through the plug 6 to prevent the syringe 5 from shaking during centrifugation.

[0023] The method for preparing PRP is as follows:

[0024] I. Required materials

[0025] 1. Disposable sterile syringe 5 (20 ml syringe 5, according to sodium citrate: normal saline: blood = 2 ml: 1 ml: 17 ml);

[0026] 2. The constant temperature cylinder 2 of the present utility model;

[0027] 3. Centrifugation device 8;

[0028] 4. Three-way joint;

[0029] 5. Straight-through switch three-way row valve joint;

[0030] II. Operation steps

[0031] 1. Blood collection

[0032] Use a disposable sterile syringe 5 to draw sodium citrate and normal saline in proportion. Aspirating the sodium citrate and normal saline mixture lubricates the inner wall of the disposable sterile syringe 5, discharges the air, and collects whole blood from the patient's vein in proportion. During the collection process, it should be ensured that the collection process is sterile and the syringe 5 is in a sterile state to avoid contamination.

[0033] 2. Centrifugation

[0034] Block the sterile syringe 5 containing collected whole blood with a sterile two-way connector. The plunger of the syringe 5 can be cut from the syringe 5. Place the syringe 5 into the thermostatic cylinder 2 of the present utility model, connect the power supply 7, set the temperature to 38 degrees, and immediately place it into a centrifuge. Set the rotational speed of the centrifuge to 1800 rpm (or set the corresponding g value according to the operating instructions of the specific centrifuge), and the centrifugation time to 10 minutes. The first separation of whole blood is completed.

[0035] After centrifugation, the blood in the centrifuge tube will be divided into three layers: the uppermost layer is platelet-poor plasma (PPP), the middle layer is PRP, and the lowermost layer is red blood cells. Connect a straight-through switch three-way row valve joint to the syringe 5 after the first centrifugation. Use three sterile syringes 5 to separately aspirate the serum, the upper layer, the middle layer, the lower layer serum, the upper 0.5 cm white membrane layer, and the red blood cells, avoiding aspirating more red blood cells (the amount of each layer aspirated during the aspiration process can be determined by referring to the digital changes of the photoelectric sensor for checking the liquid color).

[0036] 3. Transfer the aspirated middle layer, lower layer serum, upper 0.5 cm white membrane layer, and red blood cells to a sterile syringe 5. Set the rotational speed of the centrifuge to 2600 rpm according to the previous centrifugation method and centrifuge for 6 minutes. Then connect a two-way connector to draw blood, and the lower half part is the final PRP, avoiding drawing red blood cells. It can be used for subsequent treatment or experiments. Note that strict aseptic operation should be carried out during the operation process to avoid contamination and damage of PRP.

[0037] III. Precautions

[0038] 1. During the whole operation process, strict aseptic conditions must be maintained to prevent blood contamination and infection.

[0039] 2. The rotational speed and time of the centrifugation device 8 should be adjusted according to the specific equipment to obtain the best PRP separation effect.

[0040] 3. Since a low-dose anticoagulant is used, the blood may coagulate easily during centrifugation. Therefore, centrifugation should be carried out immediately after blood collection.

[0041] 4. When aspirating PRP, red blood cells should be avoided from being aspirated to avoid affecting the quality and effect of PRP.

[0042] IV. Conclusion

[0043] With the constant-temperature cylinder 2 of the present utility model, without using special test tubes and adding a low dose of anticoagulant, a disposable sterile syringe 5 can be used in cooperation with a self-made temperature control protection outer sleeve tube for the sterile syringe 5, a three-way joint, and a straight-through switch three-way row valve joint. The sterile syringe 5 is a Class III medical device that has been used clinically for a long time. It is sterile and safe and does not need to participate in the preparation of other test tubes. As long as the aseptic operation is ensured during the preparation process, the quality of the prepared PRP is higher and the safety is better. This method is simple and practical. Except for autologous blood, only a very small amount of sodium citrate and normal saline are involved, and the dosage of the participating substances is lower, which is equivalent to the low-dose skin test solution for allergic reaction test participating in contacting PRP. It is applicable to various occasions where PRP needs to be prepared. It can ensure the safety of PRP more effectively. However, it should be noted that due to the use of a low dose of anticoagulant and the cooperation of a centrifugal constant-temperature device, more care is needed during the operation to avoid blood or PRP coagulation and contamination.

Claims

1. A constant-temperature PRP centrifugation assembly, characterized in that, It includes a constant temperature cylinder (2), the outer diameter of the constant temperature cylinder (2) is equal to the inner diameter of the centrifuge cylinder (1). During use, the constant temperature cylinder (2) is placed inside the centrifuge cylinder (1) of the PRP centrifuge device (8), and the constant temperature cylinder (2) is driven to rotate by the PRP centrifuge device (8) to complete the centrifugation operation; The constant temperature cylinder (2) includes an independently arranged syringe centrifuge chamber (21) and a power supply chamber (22). The tops of the syringe centrifuge chamber (21) and the power supply chamber (22) are open. The syringe centrifuge chamber (21) is used to place the syringe (5) containing blood, and the power supply chamber (22) is used to place the power supply (7); an electric heating wire (4) is wound around the outer wall of the syringe centrifuge chamber (21), and the electric heating wire (4) is electrically connected to the power supply (7). The electric heating wire (4) is used to heat the syringe centrifuge chamber (21) during centrifugation; the power supply (7) is provided with a switch for controlling the on / off of the electric heating wire (4) and a heating gear adjustment button.

2. The constant-temperature PRP centrifugation assembly according to claim 1, wherein, The heating gear adjustment button adopts button adjustment or knob adjustment.

3. The constant-temperature PRP centrifugation assembly according to claim 1, wherein, A cylindrical plug (6) is also inserted at the top opening (221) of the syringe centrifuge chamber (21). The bottom of the plug (6) abuts against the syringe (5), and the syringe (5) is fixed inside the syringe centrifuge chamber (21) through the plug (6) to prevent the syringe (5) from shaking during centrifugation.

4. The constant-temperature PRP centrifugation assembly according to claim 1, wherein, The side of the power supply chamber (22) is provided with an opening (221) for the power supply (7) wire to pass through and connect to the electric heating wire (4).

5. The constant-temperature PRP centrifugation assembly according to claim 1, wherein, The outer wall of the syringe centrifuge chamber (21) is prefabricated with a spiral wire groove, and the electric heating wire (4) is arranged along the wire groove; an adhesive tape is pasted on the outer surface of the syringe centrifuge chamber (21) to fix the electric heating wire (4).

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