Wastewater toxicity detection equipment based on chlorella photosynthesis oxygen production rate

Abstract

The utility model relates to the field of wastewater toxicity detection, and discloses wastewater toxicity detection equipment based on chlorella photosynthesis oxygen production rate, which comprises a magnetic stirrer (1) and a reactor arranged on the magnetic stirrer (1), the reactor comprises an outer shell (2) and an inner shell (3), a detection chamber (4) for detecting wastewater is formed in the inner shell (3), and the inner shell (3) is internally provided with a water inlet (5) and a water outlet (6). A gap between the outer shell (2) and the inner shell (3) forms a cooling chamber (5) for providing a constant-temperature environment for the detection chamber (4), a magnetic rotor (6) is arranged at the bottom of the detection chamber (4), and a dissolved oxygen probe (7) is arranged at the upper part of the detection chamber (4); a light source (11) is arranged outside the cooling chamber (5), and a light shielding device is arranged outside the light source (11), so that the influence of exogenous light on the photosynthesis of algae is eliminated, the oxygen production rate of the photosynthesis of chlorella is detected, and the toxicity of wastewater is rapidly and sensitively detected.

Description

Technical Field

[0001] The utility model relates to the field of wastewater toxicity detection, and particularly relates to a wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis. Background Art

[0002] Industrial production often discharges toxic pollutants such as heavy metals (copper, zinc, cobalt, etc.), phenols, pesticides, etc. These toxic pollutants discharged into the water environment will pose serious safety hazards. The industry commonly uses the luminous bacteria method, zebrafish method, cell culture method, and microbial respiration method to detect the toxicity of wastewater. However, these technologies have defects such as cumbersome operation, expensive equipment, long test time, unstable test results, and inability to directly evaluate ecological toxicity. Algae are an important part of the water environment, are the primary producers of the food chain in the water body, and play a role in purifying water quality and promoting ecological balance. Detecting the toxicity of wastewater to algae can intuitively present the impact of wastewater on the water environment.

[0003] The academic community often evaluates the toxicity of wastewater through the reproduction rate of algae. For example, Fang Li et al. published the influence of Scenedesmus quadricauda on the photosynthetic activity, toxin production, and release of Microcystis aeruginosa, and Shi Yuan et al. published the research progress of toxicity testing based on algae fluorescence. This method has complex operation, large error, and long time consumption, and is difficult to be applied at the wastewater discharge site. Content of the Utility Model

[0004] Aiming at the deficiencies of the existing technology, the purpose of the utility model is to provide a wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis, which can quickly and sensitively detect the toxicity of wastewater.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis includes a magnetic stirrer and a reactor installed on the magnetic stirrer. The reactor includes an outer shell and an inner shell. A detection chamber for detecting wastewater is formed inside the inner shell. The gap between the outer shell and the inner shell forms a cooling chamber for providing a constant temperature environment for the detection chamber. A magnetic rotor is arranged at the bottom of the detection chamber, and a dissolved oxygen probe is arranged at the upper part thereof to detect the oxygen production rate of Chlorella photosynthesis; a light source is arranged outside the cooling chamber, and a light shielding device is arranged outside the light source to exclude the influence of external light on the photosynthesis of algae.

[0007] Preferably, the outer shell is connected with a water inlet pipe and a water outlet pipe. The water pipe is located at the bottom of the outer shell, and the water outlet pipe is located in the upper middle part of the outer shell. The water inlet pipe is connected to a circulating cooling water device to provide a constant temperature environment for the detection chamber.

[0008] Preferably, an aeration device is further arranged in the detection chamber for removing the dissolved oxygen in the wastewater to a low concentration by nitrogen aeration.

[0009] Preferably, the outer housing and the inner housing are transparent housings, and the light source is a spiral light source arranged around the outer wall of the outer housing to provide a balanced and stable light source for the detection chamber.

[0010] Preferably, the reactor further includes a lid that matches the outer housing and the inner housing, and the lid is opened to facilitate the addition of Chlorella vulgaris liquid and reagents into the detection chamber.

[0011] Preferably, a plug is provided at the connection between the dissolved oxygen probe and the lid to ensure that the detection chamber and the cooling chamber are in a closed space.

[0012] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0013] The detection device of the present utility model uses Chlorella vulgaris as a model organism, and uses a dissolved oxygen probe to detect the oxygen production rate of Chlorella vulgaris photosynthesis. During the detection process, circulating cooling water is used to ensure a constant temperature, and a spiral light source is used to provide a high-intensity and stable light source to achieve rapid and sensitive detection of the toxicity of wastewater. Algae can quickly generate oxygen through photosynthesis. By detecting the inhibition of the oxygen production rate of algae photosynthesis by wastewater, the toxicity detection of wastewater can be completed quickly within 20 minutes. Chlorella vulgaris is an alga widely present in natural water bodies and is used as a model organism without the need for screening, domestication or special cultivation of strains. Using Chlorella vulgaris to evaluate the ecological toxicity of wastewater has wide applicability, and the detection results of toxicity have strong comparability. Description of the Drawings

[0014] Figure 1 It is a schematic structural diagram of the wastewater toxicity detection device.

[0015] Figure 2 It is a schematic structural diagram of the reactor in the wastewater toxicity detection device.

[0016] Figure 3 It is a schematic structural diagram of the reactor in the wastewater toxicity detection device.

[0017] In the drawings: 1 - magnetic stirrer, 2 - outer housing, 3 - inner housing, 4 - detection chamber, 5 - cooling chamber, 6 - magnetic rotor, 7 - dissolved oxygen probe, 8 - water inlet pipe, 9 - water outlet pipe, 10 - aeration device, 11 - light source, 12 - lid, 13 - plug. Detailed Embodiments

[0018] Embodiment 1: A preferred embodiment of the present utility model provides a wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis, including a magnetic stirrer 1 and a reactor installed on the magnetic stirrer 1. The reactor includes an outer shell 2 and an inner shell 3. A detection chamber 4 for detecting wastewater is formed inside the inner shell 3. The gap between the outer shell 2 and the inner shell 3 forms a cooling chamber 5 for providing a constant temperature environment for the detection chamber 4. A magnetic rotor 6 is provided at the bottom of the detection chamber 4 to uniformly mix the algae and dissolved oxygen in the solution by magnetic stirring. A dissolved oxygen probe 7 is provided at its upper part to detect the oxygen production rate of Chlorella photosynthesis; A light source 11 is provided outside the cooling chamber 5 to provide the light source 11 for the detection chamber 4 and increase the photosynthesis rate of Chlorella. At the same time, a light-shielding device is provided outside the light source 11 to exclude the influence of external light on the photosynthesis of algae.

[0019] The outer shell 2 is connected with a water inlet pipe 8 and a water outlet pipe 9. The water inlet pipe 8 is located at the bottom of the outer shell 2, and the water outlet pipe 9 is located in the upper middle part of the outer shell 2. The water inlet pipe 8 is connected to a circulating cooling water device 10 for providing a constant temperature environment for the detection chamber 4; The device also includes an aeration device arranged in the detection chamber 4 for removing the dissolved oxygen in the wastewater to a low concentration by nitrogen aeration.

[0020] The diameter-height ratio of the detection chamber 4 is 0.75 - 2. A dissolved oxygen detector is provided above the detection chamber 4 for real-time detection of the dissolved oxygen concentration in the solution.

[0021] Embodiment 2: A preferred embodiment of the present utility model provides a wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis. The difference from the above embodiment is only that: the outer shell 2 and the inner shell 3 are transparent shells, and the light source 11 is a spiral light source arranged around the outer wall of the outer shell 2 to provide a balanced and stable light source 11 for the detection chamber 4; A light-shielding device is provided outside the spiral light source. The light-shielding device is an opaque cover arranged on the spiral light source to exclude the influence of other light sources on the photosynthesis of algae.

[0022] Embodiment 3: A preferred embodiment of the present utility model provides a wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis. The difference from the above embodiment is only that: the reactor further includes a lid 12 matching the outer shell 2 and the inner shell 3. The lid 12 is opened to facilitate adding Chlorella liquid and reagents into the detection chamber 4. A plug 13 is provided at the connection between the dissolved oxygen probe 7 and the lid 12 to ensure that the detection chamber 4 and the cooling chamber 5 are in a closed space.

[0023] The specific detection method is as follows: Using Chlorella vulgaris as a model organism to rapidly detect the toxicity of wastewater. ① Use nitrogen aeration to remove the dissolved oxygen in the wastewater to be tested to a low concentration, and the dissolved oxygen concentration of the water sample to be tested is 2-3 mg / L. ② Add a certain volume of wastewater with dissolved oxygen removed to the detection chamber, and add an appropriate amount of sodium bicarbonate to the water sample. ③ Add the Chlorella vulgaris solution with dissolved oxygen removed to the detection chamber (the COD concentration of the added Chlorella vulgaris is 100-300 mg / L, and the mass concentration is 0.1-0.3 g / L), and proliferate it through a nutrient solution containing sodium nitrate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, and trace elements. ④ Place the dissolved oxygen probe into the solution, and use a stopper 13 (such as a rubber stopper) to ensure the airtightness of the detection chamber 4. ⑤ Under the condition of magnetic stirring, turn on the light source 11 and record the concentration of dissolved oxygen. ⑥ According to the percentage difference between the oxygen production rate of the algal solution in the wastewater and the oxygen production rate of the algal solution in pure water during the light source turning-on stage, it is used as the toxicity detection result of the wastewater.

[0024] The calculation method of biological toxicity is: 100%·(k S -k C ) / k S .

[0025] Among them, k S is the oxygen production rate of the aqueous solution photosynthesis of the algal solution, and k c is the oxygen production rate of the photosynthesis of the algal solution in the wastewater solution.

Claims

1. A wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis, characterized in that, It includes a magnetic stirrer (1) and a reactor installed on the magnetic stirrer (1). The reactor includes an outer housing (2) and an inner housing (3). A detection chamber (4) for detecting wastewater is formed inside the inner housing (3). The gap between the outer housing (2) and the inner housing (3) forms a cooling chamber (5) for providing a constant temperature environment for the detection chamber (4). A magnetic rotor (6) is provided at the bottom of the detection chamber (4), and a dissolved oxygen probe (7) is provided at its upper part to detect the oxygen production rate of Chlorella photosynthesis. A light source (11) is provided outside the cooling chamber (5), and a light-shielding device is provided outside the light source (11) to exclude the influence of external light on algal photosynthesis.

2. The wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis according to claim 1, characterized in that, The outer housing (2) is connected with a water inlet pipe (8) and a water outlet pipe (9). The water inlet pipe (8) is located at the bottom of the outer housing (2), and the water outlet pipe (9) is located in the upper middle part of the outer housing (2). The water inlet pipe (8) is connected to a circulating cooling water device (10) for providing a constant temperature environment for the detection chamber (4).

3. The wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis according to claim 2, wherein, It also includes an aeration device arranged in the detection chamber (4) for removing dissolved oxygen in the wastewater to a low concentration by nitrogen aeration.

4. The wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis according to claim 1, characterized in that, The outer housing (2) and the inner housing (3) are transparent housings, and the light source (11) is a spiral light source arranged around the outer wall of the outer housing (2) to provide a balanced and stable light source (11) for the detection chamber (4).

5. The wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis according to claim 1, wherein, The reactor also includes a lid (12) matching the outer housing (2) and the inner housing (3). The lid (12) is opened to facilitate adding Chlorella liquid and reagents into the detection chamber (4).

6. The wastewater toxicity detection device based on the oxygen production rate of Chlorella photosynthesis according to claim 5, characterized in that, A plug (13) is provided at the connection between the dissolved oxygen probe (7) and the lid (12) to ensure that the detection chamber (4) and the cooling chamber (5) are in a closed space.

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