Sample treatment and detection device with liquid transfer structure

Abstract

The utility model provides a sample treatment and detection device provided with a liquid transfer structure, which comprises an upper cover, a lower cover, a liquid transfer structure and a liquid transfer structure, a hollow containing space is formed by the base plate and the upper cover, a plurality of liquid containing spaces are formed in the base plate, a center shaft containing hole is formed in the center of the base plate, and a center shaft is detachably connected into the center shaft containing hole; the upper portion of the liquid transfer assembly penetrates through the center hole to be connected with the power device, and the liquid transfer assembly is driven by the power device to move in the vertical direction with the center shaft as the axis and / or rotate around the axis; the lower portion of the liquid transfer assembly is connected with a liquid suction head, and the liquid suction head is driven by a power device to achieve liquid transfer among different liquid containing spaces.

Description

Technical Field

[0001] The utility model relates to the field of biological technology devices, specifically, to a sample processing and detection device provided with a liquid transfer structure. Background Art

[0002] At present, the conventional diagnostic detection technology platforms mainly include microbiology, immunology and molecular detection. The main applications in medical tests include pathogenic microorganism infectious detection, tumor and genetic disease diagnosis, immune system disease diagnosis, prenatal screening and other aspects.

[0003] At present, the mainstream technology of molecular detection is fluorescence quantitative PCR technology. Due to the exponential amplification of the template by PCR technology, the aerosol contamination caused by open consumables and the need for laboratory zoning for liquid preparation, extraction, and then detection with high requirements for the clinical environment have also become important factors restricting the further application of fluorescence quantitative PCR technology. At the same time, traditional products have many experimental steps, complex operations, PCR aerosol contamination, low automation, and manual operations are extremely likely to lead to unstable results and great difficulty in realizing multiplex detection.

[0004] With the development of technology, the demand for the development of molecular diagnostic technology towards accuracy, convenience, sensitivity, automation and integration is also increasing. However, due to the complexity of its own technology in molecular detection, there are few fully automated and integrated instrument platforms from sample addition to result output, or there are many technical problems that are difficult to solve, such as complex structures, poor sensitivity and specificity, and complex operation and detection equipment. Summary of the Utility Model

[0005] Aiming at the shortcomings in the prior art that only nucleic acid detection can be achieved and the integration from sample processing to detection cannot be realized, the utility model provides a sample processing and detection device provided with a liquid transfer structure.

[0006] The utility model provides a sample processing and detection device provided with a liquid transfer structure, including:

[0007] An upper cover, a central hole is provided at the center of the upper cover;

[0008] A chassis, the chassis and the upper cover together form a hollow accommodation space, a plurality of liquid accommodation spaces are provided on the chassis, a central axis accommodation hole is provided at the center of the chassis, and a central axis is detachably connected in the central axis accommodation hole;

[0009] Liquid transfer assembly, the upper part of the liquid transfer assembly passes through the central hole and is connected to the power device, and moves vertically and / or rotates around the axis driven by the power device with the central axis as the axis; the lower part of the liquid transfer assembly is connected with a liquid suction head, and the liquid suction head realizes liquid transfer between different liquid containing spaces driven by the power device.

[0010] Further, the liquid transfer assembly includes:

[0011] A connecting part, the connecting part passes through the central hole of the upper cover and moves vertically and / or rotates around the axis in the central hole driven by the power device;

[0012] A platform part, one end of the platform part is connected with a liquid suction head, a groove with an opening downward and matching the central axis is provided at the center of the platform part, the central axis can be sleeved in the groove, and a spring is sleeved outside the groove and the central axis, the upper end of the spring is connected with the platform part, and the lower end is connected with the chassis.

[0013] The upper end of the spring is connected to the bottom surface of the platform part, and the lower end is connected to the bottom of the chassis. When the platform part moves downward under the action of an external force, the spring contracts. When the external force is withdrawn or reduced, the spring resets and drives the platform part to move upward.

[0014] Further, the power device includes a first driving device and a second driving device. The first driving device is connected to the connecting part to drive the liquid transfer assembly to move vertically and / or rotate around the axis; the second driving device passes through the connecting part and is connected to the liquid suction head for vacuum suction.

[0015] Further, a hollow cavity is provided inside the connecting part, and a sealing gasket is provided inside the connecting part. A sealed accommodation space is formed between the sealing gasket and the liquid suction head. The second driving device is communicated with the sealed accommodation space, and the liquid at the liquid suction head is inhaled or exported by the input or suction of gas.

[0016] Further, a first filter element is provided below the sealing gasket, and gas passes through the filter element and enters the liquid suction head;

[0017] Further, a sealing ring is provided between the connecting part and the central hole.

[0018] Further, an annular boss extending outward is provided on the upper cover, and a sampling port is provided on the annular boss.

[0019] Further, the sampling port is correspondingly arranged with the liquid transfer device.

[0020] Further, a sealing plug detachably connected to the sampling port is provided on the sampling port.

[0021] Furthermore, an air vent is provided on the annular convex platform.

[0022] Furthermore, a second filter element is provided in the air vent.

[0023] Furthermore, a clamping groove is provided on the outer ring of the chassis, and a clamping buckle corresponding to the clamping groove is provided on the upper cover. The clamping groove and the clamping buckle are detachably and sealingly connected.

[0024] Furthermore, a first liquid containing hole is provided on the chassis, and the first liquid containing hole is close to the edge of the chassis; between the first containing hole and the central hole, a second containing hole and a third containing hole are symmetrically provided. Fourth containing holes and fifth containing holes are provided outside the second containing hole and the third containing hole. A plurality of uniformly distributed seventh containing holes are provided on the side of the chassis opposite to the first containing hole; a sixth containing hole is provided in the area between the seventh containing hole and the fourth containing hole.

[0025] Furthermore, the chassis is integrally formed of a transparent polymer material; the transparent polymer material is an optical grade polypropylene material.

[0026] Furthermore, a limiting hole is also provided on the chassis, and the depth of the limiting hole is greater than the depth of the seventh containing hole.

[0027] The beneficial effects of the present utility model are as follows: By forming sample processing and detection reactions for liquid transfer in the detection box, it is possible to realize the automation of the preparation and detection of biological macromolecules such as nucleic acids in a closed space, overcoming problems such as cumbersome extraction and detection operations, time-consuming and laborious, inaccurate sample or reagent contamination, etc.; in addition, all extraction and detection steps can be carried out in the same closed space, shortening the detection time, and the detection results are less affected by manual operations and can be more accurate; by setting multiple detection chambers, multiple diseases can be detected simultaneously, greatly simplifying the multiple detections of clinical diagnosis of the same type of diseases. Through different detection hole settings, constant temperature amplification or PCR amplification can be carried out, with a wide range of applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic structural diagram of a sample processing and detection device with a liquid transfer structure according to the present invention;

[0029] Figure 2 It is a schematic structural diagram of a liquid suction head of a sample processing and detection device with a liquid transfer structure according to the present invention

[0030] Figure 3 It is a schematic structural diagram of the chassis of a sample processing and detection device with a liquid transfer structure according to the present invention;

[0031] Figure 4Schematic diagram of the chassis structure of the sample processing and detection device provided with a liquid transfer structure according to the present invention.

[0032] Explanation of reference numerals:

[0033] 10 Upper cover, 11 Central hole, 12 Sampling port, 13 Sealing plug, 14 Ventilation port, 15 Second filter element, 20 Liquid transfer assembly, 21 Connection part, 22 Platform part, 23 Liquid suction head, 24 Groove, 25 Bottom surface of the platform part, 26 Sealing gasket, 27 First filter element, 30 Chassis, 31 First accommodation hole, 32 Second accommodation hole, 33 Third accommodation hole, 34 Fourth accommodation hole, 35 Fifth accommodation hole, 36 Sixth accommodation hole, 37 Seventh accommodation hole, 38 Central axis accommodation hole, 39 Limiting hole, 40 Spring, 50 Central axis. Detailed implementation manners

[0034] Hereinafter, exemplary embodiments of the present utility model will be described in more detail with reference to the drawings. Although the exemplary embodiments of the present utility model are shown in the drawings, it should be understood that the present utility model can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided so that the present utility model can be more thoroughly understood and the scope of the present utility model can be completely conveyed to those skilled in the art.

[0035] Summary of the utility model

[0036] The present utility model provides a sample processing and detection device provided with a liquid transfer structure, including:

[0037] An upper cover 10, a central hole 11 is provided at the center of the upper cover 10;

[0038] A chassis 30, the chassis 30 and the upper cover 10 together form a hollow accommodation space, a plurality of liquid accommodation spaces are provided on the chassis 30, and a central axis 50 is connected to the center of the chassis 30;

[0039] A liquid transfer assembly 20, the liquid transfer assembly 20 passes through the central hole 11 and is connected to a power device, and moves vertically and / or rotates around an axis with the central axis 50 as the axis under the drive of the power device; a liquid suction head 23 is connected to the lower part of the liquid transfer assembly 20, and the liquid suction head 23 realizes liquid transfer between different liquid accommodation spaces under the drive of the power device.

[0040] Further, the liquid transfer assembly 20 includes a connecting portion 21 and a platform portion 22 connected up and down. The connecting portion 21 passes through the central hole 11 of the upper cover 10 and moves vertically and / or rotates around the axis in the central hole 11 driven by the power device; one end of the platform portion 22 is connected with a liquid suction head 23. A downward-opening groove 24 matching the central axis is provided at the center of the platform portion 23. The central axis 50 can be sleeved in the groove 24. A spring 40 is sleeved outside the groove 24 and the central axis 50. The upper end of the spring 40 is connected with the platform portion 22, and the lower end is connected with the chassis 30.

[0041] The upper end of the spring 40 is connected to the bottom surface 25 of the platform portion, and the lower end is connected to the bottom of the chassis 30. When the platform portion moves downward under an external force, the spring contracts. When the external force is withdrawn or reduced, the spring resets to drive the platform portion to move upward.

[0042] The arrangement of the groove, the spring and the central axis sinks part of the structure of the liquid transfer assembly into the chassis, so that the overall height of the device can be reduced, and at the same time, it is ensured that there is no fixed connection structure with the chassis, and the chassis can be replaced.

[0043] The power device includes a first driving device and a second driving device. The first driving device is connected to the connecting portion to drive the liquid transfer assembly to move vertically and / or rotate around the axis; the second driving device passes through the connecting portion and is communicated with the liquid suction head for vacuum suction.

[0044] A hollow cavity is provided inside the connecting portion. A sealing gasket is provided inside the connecting portion. A sealed accommodation space is formed between the sealing gasket and the liquid suction head. The second driving device is communicated with the sealed accommodation space, and the liquid at the liquid suction head is inhaled or discharged by the input or suction of gas.

[0045] Further, a first filter element is provided below the sealing gasket, and gas passes through the filter element and enters the liquid suction head;

[0046] Further, a sealing ring is provided between the connecting portion and the central hole 11.

[0047] Further, the upper cover 10 is provided with an outward-extending annular boss, and a sampling port is provided on the annular boss.

[0048] Further, the sampling port is arranged corresponding to the liquid transfer device.

[0049] Further, a sealing plug detachably connected to the sampling port is provided on the sampling port.

[0050] Further, a ventilation port is provided on the annular boss.

[0051] Further, a second filter element is provided inside the vent hole.

[0052] Further, a clamping groove is provided on the outer ring of the chassis 30, and a clamping buckle corresponding to the clamping groove is provided on the upper cover 10. The clamping groove and the clamping buckle are detachably and sealingly connected.

[0053] Further, a sealing cover 28 is also provided on the platform part. The sealing cover 28 is used to cover the reaction holes on the chassis in the spring compression state to reduce the large evaporation of the liquid in the reaction holes due to heating.

[0054] Further, a first liquid receiving hole 31 is provided on the chassis 30. The first liquid receiving hole 31 is close to the edge of the chassis 30. Second receiving holes 32 and third receiving holes 33 are symmetrically provided with the first receiving hole 31 with respect to the central axis receiving hole 98. Fourth receiving holes 34 and fifth receiving holes 35 are provided outside the second receiving holes 32 and the third receiving holes 33. A plurality of uniformly distributed seventh receiving holes 37 are provided on the side of the chassis 30 opposite to the first receiving hole 31. A sixth receiving hole 36 is provided in the area between the seventh receiving hole 37 and the fourth receiving hole 34.

[0055] Further, the chassis 30 is integrally formed of a transparent polymer material. The transparent polymer material is an optical grade polypropylene material.

[0056] Further, a limiting hole 39 is also provided on the chassis 30. The depth of the limiting hole 39 is greater than the depth of the seventh receiving hole 37.

[0057] The liquid suction head is a hollow shell with a funnel-shaped lower part, and a through hole is provided at the bottom of the liquid suction head.

[0058] The horizontal distance between the center of the chassis and the through hole of the liquid suction head is L1. The minimum horizontal distance between each receiving hole on the chassis and the center of the chassis is Lnmin, and the maximum horizontal distance between each receiving hole on the chassis and the center of the chassis is Lnmax. Lnmin < L1 < Lnmax, where n = 1, 2, 3, 4, 5, 6, 7.

[0059] The chassis 9 is integrally formed of a transparent polymer material. The transparent polymer material can be an optical grade polypropylene material. The chassis 13 is injection-molded with an optical grade polypropylene material, which has high transparency and stable chemical properties. It is mainly used for the storage and reaction of various reagent solutions, and can also be used for fluorescence detection. Various-shaped liquid storage cavities and reaction cavities are designed on the chassis 13. The liquid storage cavities are mainly used for the preservation of reagent solutions and the storage of waste liquid during the reaction process. The reaction cavities are mainly used for the amplification and reaction of various reagents. Among them, the liquid storage cavities generally have a large volume, and the outer contour shape of the reaction cavity is conical, similar to a PCR tube, which is beneficial to closely fit with the heating block to improve the heating efficiency.

[0060] The outer walls of the various storage areas on the chassis are designed to be relatively thick to prevent liquid from permeating or volatilizing; the outer wall of the reaction area is designed to be relatively thin to improve the heat conduction efficiency during heating. The entire chassis is injection-molded from a highly transparent polymer material, with good optical transparency. Therefore, this device can also be used for the excitation and reception of reaction fluorescence.

[0061] The second, third, fourth, and fifth receiving holes can be liquid storage areas or waste liquid areas. A sealing film structure is designed at the mouth of each receiving hole. If an area is set as a storage area, it can be sealed with a film.

[0062] The first, sixth, and seventh receiving holes are liquid storage areas or reaction areas. It should be noted here that there is no strict definition of the storage area and the reaction area between the above-mentioned liquid receiving holes, and any area can be used as a storage area or a reaction area.

[0063] The reaction area can be equipped with heating equipment.

[0064] The central hole 38 is mainly used to fix the lower end of the liquid pipette tip, which is beneficial for the liquid pipette tip to sink and suck liquid without deviation, and at the same time can also reduce the overall height of the device. The design purpose of the limiting hole 33 is to raise the seventh receiving hole 37 (10 - hole connection). Considering that the seventh receiving hole 37 is mainly used for fluorescence detection, a relatively high surface finish needs to be maintained. By lengthening the limiting hole 39 to raise the seventh receiving hole 37, this can prevent the seventh receiving hole 37 from rubbing against the desktop and thus generating scratches.

[0065] The application scope of the above sample processing and detection device provided with a liquid transfer structure includes but is not limited to: being applicable to the integrated design of microbial, immunological, and molecular detection devices.

[0066] Taking the simultaneous detection of multiple respiratory diseases as an example, when using the above device, the specific method is as follows:

[0067] 1) Liquid encapsulation and storage: The first receiving hole stores the magnetic bead solution; the second receiving hole stores the lysis solution; the third receiving hole stores the washing solution; the fourth receiving hole stores the reconstitution buffer; the fifth receiving hole stores the lyophilized reverse transcriptase; the seventh receiving hole (10 holes) respectively store the lyophilized PCR enzymes corresponding to multiple respiratory pathogen microorganisms.

[0068] 22) Reaction process:

[0069] 21) Add the sample containing bacteria to the No. 1 hole;

[0070] 22) The pipette tip transfers a certain amount of lysis solution from the No. 2 hole to the No. 1 hole, and at the same time, the No. 1 hole is heated for a period of time to lyse the bacteria and release RNA;

[0071] 23) During the heating process of Well 1, the pipette tip transfers the reconstitution buffer in Well 4 to Wells 5 and 7 to dissolve the freeze-dried enzyme inside.

[0072] 24) After the heating of Well 1 is completed, the pipette tip transfers the waste liquid in Well 1 to Well 2.

[0073] 25) The pipette tip transfers the cleaning solution in Well 3 to Well 1, and the pipette tip blows and aspirates repeatedly in Well 1 to complete thorough cleaning.

[0074] 26) The pipette tip transfers the waste liquid in Well 1 to Well 3.

[0075] 27) The pipette tip transfers the reverse transcriptase solution in Well 5 to Well 1. At the same time, Well 1 is heated for a period of time to reverse transcribe RNA into DNA.

[0076] 28) Transfer the DNA solution in Well 1 to Well 7 and mix it with the PCR enzyme therein. At the same time, Well 7 is heated cyclically at 50 - 95 °C.

[0077] 29) During the cyclic heating process of Well 7, the excitation light of the supporting instrument is incident into Well 7 to collect the fluorescence of Well 7. Independent analysis is performed on the fluorescence signals of each well. Finally, the diagnostic result of the respiratory pathogen microorganisms contained in the sample can be obtained.

[0078] In the present invention, through the sample processing and detection reaction of liquid transfer formed in the detection kit, it is possible to realize the automation integration of the preparation and detection of biological macromolecules such as nucleic acids in a closed space, overcoming problems such as cumbersome extraction and detection operations, time-consuming and laborious, inaccurate sample or reagent contamination, etc.; in addition, all extraction and detection steps can be carried out in the same closed space, shortening the detection time required, and the detection result is less affected by manual operation and can be more accurate; by setting multiple detection chambers, multiple diseases can be detected simultaneously, greatly simplifying the multiple detections of clinical diagnosis of the same type of diseases. Through different detection well settings, isothermal amplification or PCR amplification can be carried out, with the characteristic of wide application range.

[0079] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present utility model, rather than to limit it; although the present utility model has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present utility model.

Claims

1. A sample processing and detection device provided with a liquid transfer structure, characterized in that, Comprising: An upper cover, a central hole is provided at the center of the upper cover; A chassis, the chassis and the upper cover together form a hollow accommodation space, a plurality of liquid accommodation spaces are provided on the chassis, a central axis accommodation hole is provided at the center of the chassis, and a central axis is detachably connected in the central axis accommodation hole; A liquid transfer assembly, the upper part of the liquid transfer assembly passes through the central hole and is connected to a power device, and drives the liquid transfer assembly to move vertically and / or rotate around the axis with the central axis as the axis under the drive of the power device; a liquid suction head is connected to the lower part of the liquid transfer assembly, and the liquid suction head realizes liquid transfer between different liquid accommodation spaces under the drive of the power device.

2. The sample processing and detection device provided with a liquid transfer structure according to claim 1, wherein, The liquid transfer assembly includes: A connecting part, the connecting part passes through the central hole of the upper cover and drives the liquid transfer assembly to move vertically and / or rotate around the axis in the central hole under the drive of the power device; A platform part, one end of the platform part is connected with a liquid suction head, a downward-opening groove matching the central axis is provided at the center of the platform part, the central axis can be sleeved in the groove, and a spring is sleeved outside the groove and the central axis, the upper end of the spring is connected to the platform part, and the lower end is connected to the chassis.

3. The sample processing and detection device provided with a liquid transfer structure according to claim 2, wherein, The power device includes a first driving device and a second driving device, the first driving device is connected to the connecting part to drive the liquid transfer assembly to move vertically and / or rotate around the axis; the second driving device passes through the connecting part and is communicated with the liquid suction head for vacuum suction.

4. The sample processing and detection device provided with a liquid transfer structure according to claim 2, wherein, A hollow cavity is provided inside the connecting part, a sealing gasket is provided inside the connecting part, a sealed accommodation space is formed between the sealing gasket and the liquid suction head, and the second driving device is communicated with the sealed accommodation space.

5. The sample processing and detection device provided with a liquid transfer structure according to claim 4, wherein, A first filter element is provided below the sealing gasket, and gas passes through the filter element and enters the liquid suction head.

6. The sample processing and detection device provided with a liquid transfer structure according to claim 2, wherein, A sealing ring is provided between the connecting part and the central hole.

7. The sample processing and detection device provided with a liquid transfer structure according to claim 1, characterized in that, An outwardly extending annular boss is provided on the upper cover, and a sample inlet is provided on the annular boss; the sample inlet is arranged corresponding to the liquid transfer device.

8. The sample processing and detection device provided with a liquid transfer structure according to claim 7, wherein, A sealing plug detachably connected to the sample inlet is provided on the sample inlet.

9. The sample processing and detection device provided with a liquid transfer structure according to claim 7, characterized in that, A ventilation port is provided on the annular boss.

10. The sample processing and detection device provided with a liquid transfer structure according to claim 9, characterized in that, A second filter element is provided in the ventilation port.

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