Exosome extraction device

Abstract

The utility model relates to an exosome extraction device, and belongs to the technical field of cell experiments, the exosome extraction device comprises a centrifugal machine and an extraction pipe located in the centrifugal machine, a centrifugal device is assembled on the centrifugal power end of the centrifugal machine, and a placing hole for the extraction pipe to be inserted and stored is formed in the upper portion of the centrifugal device; an end cover is detachably mounted at the top of the extraction tube, a filtering structure is arranged on the end cover, and the filtering structure comprises a filter screen connected to the end cover. According to the exosome extraction device, exosomes precipitated at the lower end are blocked by arranging the filter screen in the extraction pipe, exosome leakage caused in the process of taking out redundant supernate is reduced, and the social group filter screen shell is detachably connected relative to the end cover, so that on one hand, the separation influence of the filter screen on the exosomes in the centrifugal process can be reduced; on the other hand, the filter screen can conveniently extend to the lower end of the extraction pipe to block the exosome, and the practicability of the device is improved.

Description

Technical Field

[0001] This application relates to the technical field of cell experiment techniques, and specifically provides an exosome extraction device. Background Art

[0002] Exosome extraction is a process for extracting exosomes from cell culture supernatants or biological tissue. Exosomes are small vesicles secreted by cells, containing biomolecules such as proteins, nucleic acids, and lipids, and having important functions in cell-to-cell communication and regulation. Exosome extraction is usually used in fields such as studying cell-to-cell communication, disease diagnosis, and drug delivery.

[0003] Upon retrieval, the utility model with the publication number CN217230718U, an exosome extraction device, includes a working platform. A driving motor is fixedly connected inside the working platform. The upper end of the driving motor extends to be provided with a motor shaft, and the upper end of the motor shaft is fixedly connected with a bracket. One side contour of the bracket is "J"-shaped. The upper end of the bracket is rotatably connected with a collar. The axis of the motor shaft and the rotation center line of the collar are not coplanar. A separation container is placed on the collar. The separation container includes a container cylinder. The lower part of the container cylinder shrinks to form a collection chamber. A fixed ring is slidably arranged inside the container cylinder. A filter screen is fixedly connected to the middle of the fixed ring. A first spring is arranged below the fixed ring, and the lower end of the first spring abuts against the inclined surface formed by the lower part of the container cylinder shrinking. In this utility model, during the filtering process, the filter screen can move reciprocally, reducing the probability of the filter screen being blocked and improving the filtering efficiency.

[0004] The above-mentioned patent application proposes that the separation container has a convergence chamber and is convenient for users to extract exosomes through a channel. However, after the cell culture supernatant undergoes pretreatment centrifugation and high-speed centrifugation, the exosome precipitate will aggregate on the inner bottom wall of the container. Currently, we use 200 μl of 1xPSB to uniformly blow and beat the centrifuged precipitate to resuspend it into a suspension for exosome extraction. Based on the adhesion of the above-mentioned exosome precipitate, using the above reference technical solution, it is easy to cause exosomes to remain in the extraction device and is very difficult to clean. Therefore, an exosome extraction device is proposed to solve the above-mentioned problems. Utility Model Content

[0005] Aiming at the deficiencies of the prior art, this application provides an exosome extraction device, which has advantages such as being convenient for blowing and beating exosomes to form a resuspended solution for extraction.

[0006] To achieve the above object, this application provides the following technical solution: An exosome extraction device includes a centrifuge and an extraction tube located inside the centrifuge. A centrifugator is assembled on the centrifugal power end of the centrifuge. A placement hole for inserting and storing the extraction tube is opened above the centrifugator;

[0007] A end cap is detachably installed at the top of the extraction tube. A filtering structure is arranged on the end cap. The filtering structure includes a filter screen connected to the end cap. The filter screen fits against the inner wall of the extraction tube. A bracket is fixedly installed at the top of the filter screen. A connecting pin is fixedly connected to the top end of the bracket. The top end of the connecting pin is connected to the end cap;

[0008] An assembly bushing is fixedly installed at the bottom of the centrifuge for detachably connecting the centrifuge to the centrifuge machine. A sealing gasket is also fixedly installed on the centrifuge. A cover for positioning the extraction tube is buckled on the top of the centrifuge. A threaded rod is fixedly installed at the top of the connecting pin. An installation groove is formed at the top of the end cap. An adjusting block is rotatably installed in the installation groove. A threaded tube is fixedly installed on the adjusting block. The threaded tube is threadedly connected to the threaded rod.

[0009] Furthermore, the placement holes are arranged obliquely to the vertical direction on the centrifuge.

[0010] Furthermore, a jack for inserting the connecting pin is also formed in the installation groove. The connecting pin is inserted into the jack.

[0011] Furthermore, both the connecting pin and the jack are rectangular.

[0012] Compared with the prior art, the technical solution of the present application has the following beneficial effects:

[0013] In this exosome extraction device, a filter screen is provided inside the extraction tube to block the exosomes precipitated at the lower end, reducing the leakage of exosomes during the extraction of excess supernatant. And through the split connection of the filter screen housing relative to the end cap, on the one hand, it can reduce the influence of the filter screen on the separation of exosomes during centrifugation, and on the other hand, it can facilitate the filter screen to extend to the lower end of the extraction tube to block exosomes, improving the practicability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is the overall structure three-dimensional view of the present application;

[0015] Figure 2 is the structural schematic diagram of the centrifuge of the present application;

[0016] Figure 3 is the structural schematic diagram of the extraction tube of the present application;

[0017] Figure 4 is the structural top view of the end cap of the present application;

[0018] Figure 5 is the top view of the connection between the end cap and the adjusting block of the present application.

[0019] In the figure: 1, centrifuge; 2, centrifugator; 3, jack; 4, extraction tube; 5, gasket; 6, cover; 7, assembly bushing; 8, end cap; 9, filter screen; 10, bracket; 11, connecting pin; 12, mounting groove; 13, jack; 14, threaded rod; 15, adjusting block; 16, threaded tube. Detailed implementation manners

[0020] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0021] Please refer to Figures 1-4 , an exosome extraction device in this embodiment includes a centrifuge 1 and an extraction tube 4 located inside the centrifuge 1. Among them, a centrifugator 2 is assembled on the centrifugal power end of the centrifuge 1, and a placement hole 3 for inserting and storing the extraction tube 4 is opened above the centrifugator 2.

[0022] Preferably, the placement holes 3 are arranged obliquely to the vertical direction on the centrifugator 2.

[0023] With such a design, exosomes can effectively precipitate at the bottom of the extraction tube 4.

[0024] In this embodiment, an assembly bushing 7 is fixedly installed at the bottom of the centrifugator 2 for detachably connecting the centrifugator 2 to the centrifuge 1. A gasket 5 is also fixedly installed on the centrifugator 2, and a cover 6 for positioning the extraction tube 4 is buckled on the top of the centrifugator 2. Through the cover 6, the extraction tube 4 can be stably installed in the centrifugator 2.

[0025] It should be noted that an end cap 8 is detachably installed at the top of the extraction tube 4, and a filtering structure is provided on the end cap 8 for separating exosomes.

[0026] The filtering structure in this embodiment includes a filter screen 9 connected to the end cap 8. The filter screen 9 fits against the inner wall of the extraction tube 4. Through the blockage of the filter screen 9, the mis-taking of exosome precipitates can be reduced during the extraction of the upper suspension in the supernatant.

[0027] In this embodiment, a bracket 10 is fixedly installed at the top of the filter screen 9, a connecting pin 11 is fixedly connected to the top end of the bracket 10, and the top end of the connecting pin 11 is connected to the end cap 8.

[0028] Specifically, a threaded rod 14 is fixedly installed at the top of the connecting pin 11. An installation groove 12 is formed at the top of the end cap 8. An adjusting block 15 is rotatably installed in the installation groove 12. A threaded pipe 16 is fixedly installed on the adjusting block 15. The threaded pipe 16 is threadedly connected to the threaded rod 14.

[0029] By rotating the threaded pipe 16 located in the installation groove 12, the threaded rod 14 and the adjusting block 15 can be connected to each other, thereby realizing the assembly connection between the connecting pin 11 and the end cap 8.

[0030] It should be noted that a jack 13 for inserting the connecting pin 11 is also formed in the installation groove 12, and the connecting pin 11 is inserted into the jack 13.

[0031] Preferably, both the connecting pin 11 and the jack 13 are rectangular, so that the rotation of the connecting pin 11 relative to the jack 13 is restricted.

[0032] The working principle of the above embodiment is as follows:

[0033] The filter screen 9 is connected to the adjusting block 15 installed on the end cap 8 through the connecting pin 11, so that the filter screen 9 can be assembled on the end cap 8. At this time, the end cap 8 can be connected to the extraction tube 4 while the filter screen 9 is placed inside the extraction tube 4. The extraction tube 4 is placed in the centrifuge 2 in the centrifuge 1 for centrifugation, so that the exosomes precipitate at the bottom of the extraction tube 4. Then, by rotating the adjusting block 15 above the taken-out extraction tube 4, the adjusting block 15 drives the threaded pipe 16 to rotate relative to the threaded rod 14 during rotation, so that the filter screen 9 can be separated from the end cap 8, the filter screen 9 is pushed into the extraction tube 4, and the supernatant above the filter screen 9 is sucked with a pipette, so that the exosomes precipitate at the bottom of the extraction tube 4 through the isolation of the filter screen 9. After all the excess supernatant in the extraction tube 4 is sucked out, the filter screen 9 is taken out, and the centrifuged precipitate is evenly pipetted with 200 μl of 1xPSB to resuspend it into a suspension to achieve the extraction of exosomes.

[0034] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "including a..." does not exclude the existence of additional identical elements in the process, method, article or device including the said element.

[0035] Although embodiments of the present application have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present application. The scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An exosome extraction device, comprising a centrifuge (1) and an extraction tube (4) located within the centrifuge (1), characterized in that: A centrifuge (2) is assembled on the centrifugal power end of the centrifuge (1), and a placement hole (3) for inserting and storing the extraction tube (4) is provided above the centrifuge (2); A end cap (8) is detachably installed at the top of the extraction tube (4), and a filtering structure is arranged on the end cap (8). The filtering structure includes a filter screen (9) connected to the end cap (8). The filter screen (9) fits against the inner wall of the extraction tube (4). A bracket (10) is fixedly installed at the top of the filter screen (9), and a connecting pin (11) is fixedly connected to the top of the bracket (10). The top of the connecting pin (11) is connected to the end cap (8); A threaded rod (14) is fixedly installed at the top of the connecting pin (11). An installation groove (12) is provided at the top of the end cap (8). An adjusting block (15) is rotatably installed in the installation groove (12). A threaded tube (16) is fixedly installed on the adjusting block (15). The threaded tube (16) is threadedly connected to the threaded rod (14). A jack (13) for inserting the connecting pin (11) is also provided in the installation groove (12), and the connecting pin (11) is inserted into the jack (13).

2. The exosome extraction device according to claim 1, wherein: The placement holes (3) are arranged obliquely to the vertical direction on the centrifuge (2).

3. An exosome extraction device according to claim 1, characterized in that: An assembly bushing (7) is fixedly installed at the bottom of the centrifuge (2) for detachably connecting the centrifuge (2) to the centrifuge (1). A sealing gasket (5) is also fixedly installed on the centrifuge (2). A cover (6) for positioning the extraction tube (4) is buckled on the top of the centrifuge (2).

4. The exosome extraction device according to claim 1, wherein: Both the connecting pin (11) and the jack (13) are rectangular.

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