Multi-layer filter bottle

Abstract

The utility model discloses a multi-layer filter bottle which comprises a collecting bottle and a filter device connected to a bottle opening of the collecting bottle, the filtering device comprises a first filtering structure and a second filtering structure which are detachably connected with each other; the first filtering structure comprises a first cup body and a connecting opening connected with a bottle opening of the collecting bottle; the second filtering structure comprises a second cup body and a first connecting part connected with the upper end of the first cup body, the first cup body further comprises a second connecting part arranged towards the second cup body, and the first connecting part is detachably connected with the second connecting part; a first filtering piece is arranged in a cavity in the first cup body, and a second filtering piece is arranged in a cavity in the second cup body. According to the utility model, the filter sheets with different pore diameters can be conveniently replaced, cells can be conveniently filtered, the risk that the filter sheets are blocked is reduced, and meanwhile, the filtering effect and the filtering efficiency are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell and tissue culture, and particularly relates to a multi-layer filtering bottle. Background Art

[0002] During the cell culture process, cells secrete active substances such as cytokines into the culture medium. For example, in addition to beneficial components such as cytokines, a stem cell culture medium also contains harmful substances such as waste products of cell growth and metabolism, dead cells, and cell debris. Filtration treatment is required before use. Generally, a cell filter disc is used for filtration. Since the cell sieve purchased on the market has a small aperture and the volume of the culture medium to be filtered is large, a thin film is easily formed on the filter mesh. The thin film and harmful substances are likely to cause blockage of the filter mesh. During the operation process, multiple cell filter discs are needed, which is not conducive to aseptic operation and affects the filtration effect and efficiency.

[0003] Existing filtering devices are also used in aspects such as laboratory cell culture, filtration of culture media and reagents. For example, when extracting placenta and umbilical cord mesenchymal stem cells by the digestion method, they have a characteristic that the specimens are large and the number of digested cells is huge. If a large amount of liquid needs to be filtered, first, it takes a lot of time, and second, multiple filter discs may be used in the experiment, with high costs, inability to install filter discs of different specifications, easy to fall off during screening, inability to perform multi-layer screening, and easy to generate errors. Content of the Utility Model

[0004] The present application provides a multi-layer filtering bottle, which is used to conveniently replace filter discs with different apertures, reduce the risk of filter disc blockage, and improve the filtration effect and filtration efficiency at the same time.

[0005] The multi-layer filtering bottle provided by the present application includes a collection bottle and a filtering device connected to the bottle mouth of the collection bottle; the filtering device includes a first filtering structure and a second filtering structure that are detachably connected to each other; the first filtering structure includes a first cup body and a connection port connected to the bottle mouth of the collection bottle; the second filtering structure includes a second cup body and a first connection part connected to the upper end of the first cup body. The first cup body further includes a second connection part disposed towards the second cup body, and the first connection part and the second connection part are detachably connected to each other; a first filter disc is disposed in the middle cavity of the first cup body, and a second filter disc is disposed in the cavity of the second cup body.

[0006] By adopting the above technical solution, filtration of cells is achieved through a multi-layer filtration method. Among them, the prepared stem cell culture medium is poured into the second cup body. Through the filtration of the second filter sheet, the protein fiber substances suspended in the culture medium will be retained in the second cup body. Then, it undergoes sterilization filtration through the first filter sheet, and finally, stem cells are obtained and stored in the collection bottle. This solution has quick steps, reduces the risk of filter sheet blockage, and improves the filtration effect and efficiency at the same time.

[0007] Optionally, an external thread is provided on the outer side of the first connecting portion, and an internal thread that is screwed tightly with the external thread is provided on the inner side of the second connecting portion.

[0008] By adopting the above technical solution, the connection stability between the first connecting portion and the second connecting portion is improved, and thus the first cup body and the second cup body can be detachably connected to each other.

[0009] Optionally, the pore size of the first filter sheet is smaller than the pore size of the second filter sheet.

[0010] By adopting the above technical solution, since the second cup body filters the protein fiber substances suspended in the culture medium and the first cup body filters the bacteria in the culture medium, the pore size of the first filter sheet is smaller than the pore size of the second filter sheet.

[0011] Optionally, different models of the first cup body are fixed to different pore sizes of the first filter sheet, and different models of the second cup body are fixed to different pore sizes of the second filter sheet; the internal threads of each first cup body located on the second connecting portion are screwed tightly corresponding to the external threads of each second cup body located on the first connecting portion.

[0012] By adopting the above technical solution, due to different cultured protein fibers in the culture medium and different varieties of bacteria in the culture medium, it can be achieved according to different combinations of the first cup body and the second cup body, which facilitates the filtration of stem cells in the culture medium.

[0013] Optionally, a first thread is provided at the bottle mouth of the collection bottle, and a second thread that is screwed tightly with the first thread is provided at the connection port of the first cup body.

[0014] By adopting the above technical solution, the connection stability between the first bottle body and the bottle mouth of the collection bottle is improved.

[0015] Optionally, a bottle cap is provided at the bottle mouth of the collection bottle, and a second thread that is screwed tightly with the first thread is provided on the bottle cap.

[0016] By adopting the above technical solution, when the filtration of stem cells is completed, the bottle mouth of the collection bottle is sealed by the bottle cap, thereby reducing the entry of bacteria and dust into the collection bottle.

[0017] Optionally, a detachable cup lid is provided at the opening of the second cup body.

[0018] One or more technical solutions provided in this application have at least the following technical effects or advantages:

[0019] 1. This application uses the first filtering structure and the second filtering structure to perform multiple filtrations on the stem cells in the culture medium. The steps of the solution are fast, reducing the risk of filter sheet blockage, and at the same time improving the filtering effect and filtering efficiency.

[0020] 2. This application improves the stability of the detachable connection between the first connecting portion and the second connecting portion through the detachable connection between the first cup body and the second cup body and the threaded connection between the first connecting portion and the second connecting portion.

[0021] 3. In this application, due to the different cultured protein fibers in the culture medium and the different bacterial species in the culture medium, it can be achieved according to different combinations of the first cup body and the second cup body, thereby facilitating the filtration of stem cells in the culture medium. Description of the Drawings

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model, the following will briefly introduce the drawings required for description in the embodiments. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0023] Figure 1 It is a schematic diagram of the overall structure of the multi-layer filtration bottle in the embodiment of this application;

[0024] Figure 2 It is an exploded structure diagram of the multi-layer filtration bottle in the embodiment of this application;

[0025] Figure 3 It is an exploded structure diagram of the multi-layer filtration bottle in the embodiment of this application.

[0026] Explanation of the reference numerals in the drawings: 1. Collection bottle; 11. First thread; 2. Filtration device; 21. First filtration structure; 211. First cup body; 212. Connection port; 2121. Second thread; 213. First filter sheet; 214. Second connecting portion; 2141. Internal thread; 22. Second filtration structure; 221. Second cup body; 222. First connecting portion; 2221. External thread; 223. Second filter sheet; 224. Cup lid. Detailed Embodiments

[0027] The present application provides a multi-layer filtration bottle, which is used to facilitate the replacement of filter sheets with different pore sizes, facilitate the filtration of cells, reduce the risk of filter sheet clogging, and improve the filtration effect and filtration efficiency.

[0028] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present application.

[0029] It should be noted that the terms "first", "second", etc. in the description and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and do not necessarily need to be used to describe a specific order or sequence. It should be understood that such data can be interchanged under appropriate circumstances, so that the embodiments of the present application described here can be implemented in an order other than those illustrated or described here. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or server including a series of steps or units does not necessarily need to be limited to those steps or units clearly listed, but may include other steps or modules that are not clearly listed or are inherent to these processes, methods, products, or devices.

[0030] Referring to Figure 1 , a multi-layer filtration bottle in this embodiment includes a collection bottle 1 and a filtration device 2 that can be replaceably connected to the mouth of the collection bottle 1. In the present application, the replaceable filtration device 2 is used to facilitate replacement and improve the filtration effect and filtration efficiency.

[0031] Referring to Figure 2 , the filtration device 2 includes a first filtration structure 21 and a second filtration structure 22 that are detachably connected to each other. Among them, the first filtration structure 21 includes a first cup body 211 and a connection port 212 connected to the mouth of the collection bottle 1. The second filtration structure 22 includes a second cup body 221 and a first connection portion 222 connected to the upper end of the first cup body 211. Specifically, a first filter sheet 213 is arranged in the cavity of the first cup body 211, and a second filter sheet 223 is arranged in the cavity of the second cup body 221. Pour the prepared stem cell culture medium into the second cup body 221. Through the filtration of the second filter sheet 223, the protein fiber substances suspended in the culture medium will be retained in the second cup body 221, and then sterilized filtration is carried out through the first filter sheet 213, and finally stem cells are obtained and stored in the collection bottle 1. This solution is fast, reduces the risk of filter sheet clogging, and improves the filtration effect and filtration efficiency at the same time.

[0032] In addition, the first cup body 211 further includes a second connecting portion 214 disposed towards the second cup body 221, and the first connecting portion 222 and the second connecting portion 214 are detachably connected to each other. Specifically, an external thread 2221 is provided on the outer side of the first connecting portion 222, and an internal thread 2141 that is screwed tightly with the external thread 2221 is provided on the inner side of the second connecting portion 214, thereby improving the connection stability between the first connecting portion 222 and the second connecting portion 214, and further realizing the detachable connection between the first cup body 211 and the second cup body 221.

[0033] Moreover, in this application, the pore size of the first filter sheet 213 is smaller than that of the second filter sheet 223. Since the second cup body 221 filters protein fiber substances suspended in the culture medium, and the first cup body 211 filters bacteria in the culture medium, the pore size of the first filter sheet 213 is smaller than that of the second filter sheet 223.

[0034] In addition, it should be noted that different models of the first cup body 211 and various first filter sheets 213 with different pore sizes are fixed to each other, and different models of the second cup body 221 and various second filter sheets 223 with different pore sizes are also fixed to each other. And the pore size dimensions of the first filter sheet and the second filter sheet are displayed on the cup walls of the first cup body 211 and the second cup body 221, which helps to facilitate identification. Although the models of the first cup body 211 and the second cup body 221 are different, the internal thread 2141 of each first cup body 211 located on the second connecting portion 214 is screwed tightly with the external thread 2221 of each second cup body 221 located on the first connecting portion 222. By different protein fibers cultured in the culture medium and different bacterial species in the culture medium, it can be achieved according to different combinations of the first cup body 211 and the second cup body 221, thereby facilitating the filtration of stem cells in the culture medium.

[0035] Refer to Figure 2 and Figure 3 , a first thread 11 is provided at the bottle mouth of the collection bottle 1, and a second thread 2121 that is screwed tightly with the first thread 11 is provided at the connection port 212 of the first cup body 211, thereby improving the connection stability between the first bottle body and the bottle mouth of the collection bottle 1.

[0036] In addition, a bottle cap (not shown clearly in the figure) is provided at the bottle mouth of the collection bottle 1, and a second thread (not shown clearly in the figure) that is screwed tightly with the first thread 11 is provided on the bottle cap. When the filtration of stem cells is completed, the bottle mouth of the collection bottle 1 is sealed by the bottle cap, thereby reducing the entry of bacteria and dust into the collection bottle 1.

[0037] In addition to this, a detachable cup cap 224 is provided at the opening of the second cup body 221. By covering the cup cap 224, the second cup body 221 is sealed, thereby preventing the stem cell culture medium in the second cup body 221 from being contaminated.

[0038] Taking the embodiment of the present application as an example, the pore size of the first filter is 0.22 μm, and the pore size of the second filter 223 is 100 μm. Specifically, taking the filtration of 500 ml of stem cell culture medium as an example, 25 ml of cell addition factor is added to 500 ml of stem cell basal culture. After shaking up and down, it is ready for filtration. Prepare a first cup body 211 with a first filter 213 having a pore size of 0.22 μm, a second cup body 221 with a second filter 223 having a pore size of 100 μm, and a 500 ml collection bottle 1. Assemble them by rotating the connected threads. Open the cup cover 224 on the second cup body 221, pour the prepared stem cell culture medium into it, cover the cup cover 224. The protein fiber substances suspended in the culture medium will be filtered out by the second filter 223 with a pore size of 100 μm in the second cup body 221, and then flow through the first filter 213 with a pore size of 0.22 μm in the first cup body 211 for sterilization filtration, and finally stored in the 500 ml collection bottle 1. Unscrew the second connecting part 214 on the first cup body 211, and cover the collection bottle 1 with a suitable bottle cap.

[0039] In another embodiment, the multi-layer filtration bottle in the present application can be combined in other different ways. For example, a combination of 70 μm and 40 μm is used to filter live cells, or a combination of 70 μm and 0.22 μm is used for sterilization filtration of reagents such as culture media.

[0040] It should be noted that the above-mentioned sequence of embodiments of the present application is only for description and does not represent the superiority or inferiority of the embodiments. And the above description of specific embodiments of this specification is made. Other embodiments are within the scope of the appended claims. In some cases, the actions or steps recorded in the claims can be executed in a different order from that in the embodiments and still achieve the desired results. In addition, the processes depicted in the drawings do not necessarily require the specific order or continuous order shown to achieve the desired results. In certain embodiments, multi-tasking and parallel processing are also possible or may be advantageous.

[0041] The above are only the preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present application shall be included within the protection scope of the present application.

[0042] This specification and the drawings are only exemplary descriptions of the present application and are considered to cover any and all modifications, variations, combinations, or equivalents within the scope of the present application. Obviously, those skilled in the art can make various changes and modifications to the present application without departing from the scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the present application and its equivalent technologies, the present application is intended to include these changes and modifications.

Claims

1. A multi-layer filtration bottle, characterized in that, It includes a collection bottle (1) and a filtering device (2) connected to the mouth of the collection bottle (1); The filtering device (2) includes a first filtering structure (21) and a second filtering structure (22) that are detachably connected to each other; The first filtering structure (21) includes a first cup body (211) and a connection port (212) connected to the mouth of the collection bottle (1); The second filtering structure (22) includes a second cup body (221) and a first connection part (222) connected to the upper end of the first cup body (211). The first cup body (211) further includes a second connection part (214) arranged towards the second cup body (221). The first connection part (222) and the second connection part (214) are detachably connected to each other; A first filtering sheet (213) is arranged in the cavity of the first cup body (211), and a second filtering sheet (223) is arranged in the cavity of the second cup body (221).

2. The multi-layer filtration bottle according to claim 1, wherein, An external thread (2221) is arranged on the outer side of the first connection part (222), and an internal thread (2141) that is screwed tightly with the external thread (2221) is arranged on the inner side of the second connection part (214).

3. The multi-layer filtration bottle according to claim 2, wherein The aperture of the first filtering sheet (213) is smaller than the aperture of the second filtering sheet (223).

4. The multi-layer filtration bottle according to claim 3, wherein The first cup bodies (211) of different models are fixed to the first filtering sheets (213) with various apertures, and the second cup bodies (221) of different models are fixed to the second filtering sheets (223) with various apertures; The internal threads (2141) of the first cup bodies (211) located on the second connection part (214) are screwed tightly with the external threads (2221) of the second cup bodies (221) located on the first connection part (222) correspondingly.

5. The multi-layer filtration bottle according to claim 1, characterized in that, A first thread (11) is arranged at the mouth of the collection bottle (1), and a second thread (2121) that is screwed tightly with the first thread (11) is arranged at the connection port (212) of the first cup body (211).

6. The multi-layer filtration bottle according to claim 5, characterized in that, A bottle cap is arranged at the mouth of the collection bottle (1), and a second thread (2121) that is screwed tightly with the first thread (11) is arranged on the bottle cap.

7. The multi-layer filtration bottle according to claim 1, wherein A detachable cup cover (224) is arranged at the opening of the second cup body (221).

Images