Immunohistochemical pen
By designing an immunohistochemical pen with liquid storage chamber, printing drainage lines and working guides, the problem of inconsistent shape and size is solved, and the use area with adjustable shapes is achieved, which improves the consistency of dyeing results and operating efficiency.
Patent Information
- Application Number
- CN202422319574.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-23
- Publication Date
- 2025-07-29
- Estimated Expiration
- 2034-09-23
AI Technical Summary
The shape and size of existing immunohistochemical pens are inconsistent when drawing circles, resulting in a difference in the contact amount of samples and antibodies, affecting the uniformity of the staining results, and the liquid is prone to diffuse and inconvenient to operate.
An immunohistochemistry pen including a liquid storage chamber, a printed drain line and a plurality of working guides is designed to form an annular working track through the adjustment member and the elastic printed drain line, ensuring that the liquid forms a fixed-shaped use area on the slide to avoid liquid diffusion.
It realizes the adjustment of the shape and size of the use area according to experimental needs, improves the operation efficiency, ensures the consistency of the dyeing results and the liquid sealing, and simplifies the operation steps.
Smart Images
Figure CN223166441U_ABST
Abstract
Description
Technical Field
[0001] The present application belongs to the field of medical device technology, and specifically relates to an immunohistochemistry pen. Background Art
[0002] The immunohistochemistry pen is suitable for various immunohistochemical staining experiments, such as PAP method, ABC method, immunofluorescence method, frozen section and in situ hybridization technology. Draw a circle on the slide, and the immunohistochemistry pen will form a thin film on the slide to circle a certain shape of the use area, so that antibodies, probes and other reagents are limited to the designated use area and will not be lost. This can reduce the amount of antibodies and reagents used, avoid liquid flow and diffusion during staining, and increase the operation speed. The use of the immunohistochemistry pen is very effective and can simplify the operation steps.
[0003] In actual operation, circles are mostly drawn manually with an immunohistochemistry pen. The shape and size of the circled use area are random. If the same sample is drawn with circles of different sizes, the amount of contact between the sample and the antibody will be different, resulting in inconsistent staining results, which will interfere with subsequent staining results. In addition, when drawing circles, the path passed by the pen tip must have overlapping parts to close the circled use area and prevent the liquid in the use area from flowing to areas outside the use area. Utility Model Content
[0004] In order to standardize the shape of the circle drawn by the histochemistry pen and ensure the smooth progress of the experiment, the present application provides an immunohistochemistry pen.
[0005] An immunohistochemistry pen comprises a pen body provided with a liquid storage cavity, a printed drainage line and at least three working guide rails, one end of each working guide rail is connected to one end of the pen body, each working guide rail is distributed in the same plane, and the extension directions of at least two working guide rails do not overlap, and each working guide rail is also slidably connected to an adjustment member; the printed drainage line is elastic and connected to each adjustment member, each adjustment member stretches the printed drainage line so that the printed drainage line forms a ring-shaped working track, the printed drainage line is connected to the liquid storage cavity through the drainage member, the drainage member can move with the printed drainage line, and the liquid in the liquid storage cavity can be distributed on the working track through the drainage member and the printed drainage line.
[0006] In one embodiment of the present application, the immunohistochemistry pen includes two groups of working guide rails, the extension directions of the working guide rails in the two groups are different, and the extension directions of the two working guide rails in one group are the same.
[0007] In one embodiment of the present application, the printed drain wires are fixedly connected to each adjusting member.
[0008] In one embodiment of the present application, at least three working guide rails are arranged around the pen body array.
[0009] In one embodiment of the present application, the printed drainage lines located between adjacent working guide rails are respectively connected to the liquid storage cavity through a drainage member, and a plurality of drainage members are arranged in an array around the pen body.
[0010] In one embodiment of the present application, the working guide rail is provided with a guide rail long hole along its extending direction, and the depth direction of the guide rail long hole is perpendicular to the plane where the working trajectory is located; the adjusting member includes an adjusting rod, the adjusting rod passes through the guide rail long hole and can move along the extending direction of the guide rail long hole in the guide rail long hole, the adjusting rod includes a first working section and a fixed section connected to the printed drainage line; the adjusting rod is also connected to the working guide rail through a spring, so that the first working section abuts against the working guide rail, and the frictional force between the first working section and the working guide rail restricts the movement of the adjusting member along the guide rail long hole.
[0011] In one embodiment of the present application, the adjusting rod further includes a second working section, the first working section is arranged on the side of the guide rail long hole close to the working trajectory, the second working section is arranged on the side of the guide rail long hole far from the working trajectory, the spring is sleeved on the adjusting rod and is arranged between the guide rail long hole and the second working section, and the spring is in a compressed state so that the first working section abuts against the working guide rail.
[0012] In one embodiment of the present application, the working guide rail is provided with a friction layer, and the adjusting member abuts against the friction layer.
[0013] In one embodiment of the present application, the drainage member is flexible; the pen body is provided with a drainage tube, the drainage member passes through the drainage tube and is connected to the printed drainage line, and the outlet of the drainage tube is spaced from the printed drainage line and is arranged on the side of the plane where the working trajectory is located close to the pen body.
[0014] In one embodiment of the present application, the other end of the pen body is provided with an annular pen tip.
[0015] The beneficial effects of the present application at least include:
[0016] 1. By adjusting the positions of the respective adjusting members on the respective working guide rails and the elastic property of the printed drainage lines, the route of the working trajectory can be changed, and the shape of the usage area circled by the immunohistochemical pen can be changed, so as to change the shape and size of the usage area formed by the immunohistochemical pen according to experimental requirements, which is convenient for the experiment, and it is convenient for the experimenter to make a plurality of usage areas with fixed shapes through the immunohistochemical pen to meet the experimental requirements. Moreover, in the form of a circular working trajectory, it is ensured that the usage area circled by the immunohistochemical pen is closed, and the liquid is prevented from flowing to areas outside the usage area.
[0017] 2. Since the printed drainage lines are elastic, when the working trajectory is changed, the printed drainage lines can always be in a taut state, ensuring that when the printed drainage lines are printed on the glass slide, the liquid in the liquid storage cavity can be regularly printed on the glass slide through the printed drainage lines, thereby forming a usage area with a fixed shape. Brief Description of the Drawings
[0018] Figure 1 is a schematic structural diagram of an exemplary embodiment of the present application;
[0019] Figure 2 is a schematic structural diagram of an exemplary embodiment of the working trajectory in the present application;
[0020] Figure 3 is a schematic structural diagram of an exemplary embodiment of the first working section in the present application;
[0021] Figure 4 is a schematic structural diagram of an exemplary embodiment of the adjusting member in the present application;
[0022] Figure 5 is a schematic diagram when the present application is in use.
[0023] In the figures:
[0024] 101, pen body; 102, drainage tube; 103, drainage member;
[0025] 201, working guide rail; 202, guide rail long hole;
[0026] 300, adjusting member; 301, first working section; 302, second working section; 303, spring;
[0027] 401, glass slide;
[0028] 501, printed drainage line. Detailed Embodiments
[0029] For a clearer understanding of the technical features, objectives, and effects of the present application, the detailed embodiments of the present application are now described with reference to the accompanying drawings. Components with the same reference numerals in the figures represent components with the same or similar structures but the same functions.
[0030] In this document, "schematic" means "serving as an example, instance, or illustration", and any illustration or embodiment described as "schematic" in this document should not be construed as a more preferred or advantageous technical solution.
[0031] For the sake of simplicity of the drawings, only the parts related to the present application are schematically shown in each figure, and they do not represent the actual structure of the product. Additionally, for the sake of simplicity and ease of understanding of the drawings, in some figures, components with the same structure or function are only schematically shown as one of them, or only one of them is labeled.
[0032] Please refer to Figures 1 to 5 to understand the present application.
[0033] Refer toFigure 1 An immunohistochemistry pen comprises a pen body 101 with a liquid storage chamber, a printed drainage line 501, and at least three working guide rails 201. One end of each working guide rail 201 is connected to one end of the pen body 101. Each working guide rail 201 is distributed in the same plane, and the extension directions of at least two working guide rails 201 do not overlap. Each working guide rail 201 is also slidably connected to an adjustment member 300. Figure 1 One end of the pen body 101 is connected to four working guide rails 201, and each working guide rail 201 is slidably connected to an adjusting member 300. Each adjusting member 300 can move in the same plane to change the position of the adjusting member 300 in the plane.
[0034] The printed drain line 501 is elastic and connected to each adjusting member 300. Each adjusting member 300 stretches the printed drain line 501 so that the printed drain line 501 forms a circular working track. Figure 1 、 Figure 2 The printed drainage line 501 forms a square ring-shaped working track under the action of the four adjusting members 300. The printed drainage line 501 is connected to the liquid storage chamber through the drainage member 103. The drainage member 103 can move with the printed drainage line 501. The liquid in the liquid storage chamber can be distributed along the working track through the drainage member 103 and the printed drainage line 501. When the printed drainage line 501 contacts the slide 401, see Figure 5 The liquid distributed on the printed drainage line 501 can be stained on the slide 401 to form a square use area, preventing the sample from flowing to a position outside the use area. Since the adjusting member 300 can move along the working guide rail 201, and the printed drainage line 501 can be stretched by the adjusting member 300, or contracted under the action of its own elastic force, the printed drainage line 501 is always in a taut state during the movement of the adjusting member 300. The shape and size of the working track can be changed through the movement of the adjusting member 300, thereby changing the shape and size of the use area formed by the immunohistochemistry pen to achieve different experimental purposes. Those skilled in the art of the present application can understand that the printed drainage line 501 in the present application can be composed of a combination of rubber and capillary materials, which can undergo both elastic deformation and capillary action, so that the liquid in the liquid storage chamber can be distributed to the printed drainage line 501 through the drainage member 103 and can be stained on the glass slide 401; the drainage member 103 can be cotton thread, capillary tube, hose and other items, which can enable the liquid in the liquid storage chamber to reach the printed drainage line 501.
[0035] See also Figure 1, in an embodiment of the present application, the drainage member 103 is flexible; the pen body 101 is provided with a drainage tube 102, the drainage member 103 passes through the drainage tube 102 and is connected to the printed drainage line 501. The outlet of the drainage tube 102 is spaced from the printed drainage line 501 and is arranged on the side of the plane where the working track is located close to the pen body 101. The drainage tube 102 can reduce the water loss on the drainage member 103, keep the moisture of the drainage member 103, and ensure that the liquid in the liquid storage cavity can smoothly reach the printed drainage line 501.
[0036] In an embodiment of the present application, the immunohistochemical pen includes two sets of working guide rails 201. The extending directions of the working guide rails 201 in the two sets are different, and the extending directions of the two working guide rails 201 in one set are the same. Thus, by adjusting the positions of the respective adjusting members 300 on the respective working guide rails 201, rectangles of different sizes can be formed, which is convenient for the experimenter to operate and improves the experimental efficiency.
[0037] In an embodiment of the present application, the printed drainage line 501 is fixedly connected to the respective adjusting members 300. When the adjusting member 300 can stretch the printed drainage line 501, there is no relative movement between the printed drainage line 501 and the adjusting member 300, thereby ensuring the connection strength between the printed drainage line 501 and the adjusting member 300, and enabling the experimenter to stably adjust the shape of the working track.
[0038] In an embodiment of the present application, at least three working guide rails 201 are arranged in an array around the pen body 101. Further, the printed drainage lines 501 located between adjacent working guide rails 201 are respectively connected to the liquid storage cavity through a drainage member 103, and a plurality of drainage members 103 are arranged in an array around the pen body 101. Refer to Figures 1 to 3 , there are four drainage members 103 between the four adjusting members 300, so that the liquid in the liquid storage cavity can be directly dispersed to the line segment between the two adjusting members 300 through the four drainage members 103, so that the printed drainage line 501 can maintain a wet state.
[0039] Refer to Figures 1 to 4 , in an embodiment of the present application, the working guide rail 201 is provided with a guide rail long hole 202 along its extending direction. The depth direction of the guide rail long hole 202 is perpendicular to the plane where the working track is located; the adjusting member 300 includes an adjusting rod, the adjusting rod passes through the guide rail long hole 202 and can move along the extending direction of the guide rail long hole 202 in the guide rail long hole 202. The adjusting rod includes a first working section 301 and a fixed section connected to the printed drainage line 501; the adjusting rod is also connected to the working guide rail 201 through a spring 303, so that the first working section 301 abuts against the working guide rail 201, and the movement of the adjusting member 300 along the guide rail long hole 202 is restricted by the frictional force between the first working section 301 and the working guide rail 201.
[0040] Refer toFigure 4 , the spring 303 is a compression spring. The adjusting rod further includes a second working section 302. The first working section 301 is arranged on the side of the guide rail long hole 202 close to the working track, and the second working section 302 is arranged on the side of the guide rail long hole 202 far from the working track. The spring 303 is sleeved on the adjusting rod and is arranged between the guide rail long hole 202 and the second working section 302. The spring 303 is in a compressed state, so that the first working section 301 abuts against the working guide rail 201, so as to Figure 4 be described in the direction shown. The spring 303 gives an upward acting force to the second working section 302, so that the first working section 301 connected to the second working section 302 has an upward movement tendency. The diameter of the first working section 301 is larger than the diameter of the guide rail long hole 202. The first working section 301 abuts against the working guide rail 201. Thus, through the frictional force between the first working section 301 and the working guide rail 201, the movement of the adjusting member 300 along the guide rail long hole 202 is restricted. And when the printed drainage line 501 contacts the glass slide 401, the acting force between the two makes the first working section 301 move upward. However, since the working guide rail 201 restricts the upward movement of the first working section 301, the static frictional force that needs to be overcome for the relative movement between the first working section 301 and the working guide rail 201 is increased, thus avoiding the movement of the adjusting member 300 along the working guide rail 201 and making the printing process more stable. When the experimenter adjusts the position of the adjusting member 300, press the spring 303 to reduce the frictional force between the first working section 301 and the working guide rail 201, so that the first working section 301 can move relative to the working guide rail 201.
[0041] Of course, the first working section 301 can also be arranged on the side of the guide rail long hole 202 far from the working track, and the spring 303 can also be a tension spring, as long as the first working section 301 can be made to abut against the working guide rail 201.
[0042] In an embodiment of the present application, the working guide rail 201 is provided with a friction layer. The first working section 301 abuts against the friction layer. The friction layer can be made of materials such as rubber and silica gel with a relatively large friction coefficient with the first working section 301 to increase the frictional force between the first working section 301 and the working guide rail 201.
[0043] In an embodiment of the present application, one end of the pen body 101 is connected with the working guide rail 201, and the other end of the pen body 101 is provided with a circular pen tip. Thus, according to needs, one end of the pen body 101 can be selected for drawing circles. For example, when a circular use area is printed on the glass slide 401 through the circular pen tip, the liquid quality control sheet is added to the middle position of the use area. The circular hydrophobic film printed on the glass slide can block the diffusion of liquid droplets and become a barrier to block its contact with the sample to be tested, effectively avoiding the contamination of the sample to be tested.
[0044] Of course, the number of working guide rails 201 in this application is not limited to four, and can also be other numbers, such as three or five, which can form a circular working trajectory, so that the printed drainage line 501 can print a certain area of the use area on the glass slide. The more the number of working guide rails 201 and the number of adjusting members 300, the more complex the working trajectory can be realized to meet different use requirements.
[0045] It should be understood that although this specification is described according to each implementation manner, not every implementation manner only contains an independent technical solution. This narrative manner of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other implementation manners that can be understood by those skilled in the art.
[0046] The series of detailed descriptions listed above are only specific descriptions of the feasible embodiments of this application, and they are not intended to limit the protection scope of this application. Any equivalent implementation or change made without departing from the technical spirit of this application, such as the combination, division or repetition of features, should be included in the protection scope of this application.
Claims
1. An immunohistochemical pen, characterized in that, The pen comprises a pen body provided with a liquid storage cavity, a printed drainage line, and at least three working guide rails, one end of each working guide rail being connected to one end of the pen body, each working guide rail being distributed in the same plane, and at least two of the working guide rails extending in directions that do not overlap, and each working guide rail being slidably connected to an adjustment member; The printed drainage line is elastic and is connected to each of the adjusting parts. Each of the adjusting parts stretches the printed drainage line so that the printed drainage line forms a ring-shaped working track. The printed drainage line is connected to the liquid storage chamber through the drainage part. The drainage part can move with the printed drainage line. The liquid in the liquid storage chamber can be distributed to the working track through the drainage part and the printed drainage line.
2. An immunohistochemistry pen according to claim 1, characterized in that: The immunohistochemistry pen comprises two groups of working guide rails. The extending directions of the working guide rails in the two groups are different, and the extending directions of the two working guide rails in one group are the same.
3. The immunohistochemistry pen according to claim 1, characterized in that: The printed drain wires are fixedly connected to the respective adjusting members.
4. The immunohistochemistry pen according to claim 1, characterized in that: At least three of the working guide rails are arranged around the pen body array.
5. The immunohistochemistry pen according to claim 4, characterized in that: The printed drainage lines located between adjacent working guide rails are connected to the liquid storage chamber through a drainage member respectively, and a plurality of the drainage members are arranged around the pen body array.
6. The immunohistochemistry pen according to claim 1, characterized in that: The working guide rail is provided with a guide rail long hole along its extension direction, and the depth direction of the guide rail long hole is perpendicular to the plane where the working track is located; The adjusting member includes an adjusting rod, which passes through the long hole of the guide rail and can move in the long hole of the guide rail along the extension direction of the long hole of the guide rail. The adjusting rod includes a first working section and a fixed section connected to the printed drain line; The adjusting rod is also connected to the working guide rail through a spring so that the first working section abuts against the working guide rail. The friction between the first working section and the working guide rail limits the movement of the adjusting member along the long hole of the guide rail.
7. The immunohistochemistry pen according to claim 6, characterized in that: The adjusting rod also includes a second working section, the first working section is arranged on the side of the long hole of the guide rail close to the working track, and the second working section is arranged on the side of the long hole of the guide rail away from the working track. The spring is sleeved on the adjusting rod and arranged between the long hole of the guide rail and the second working section. The spring is in a compressed state so that the first working section rests against the working guide rail.
8. The immunohistochemistry pen according to claim 6, characterized in that: The working guide rail is provided with a friction layer, and the first working section abuts against the friction layer.
9. The immunohistochemistry pen according to claim 1, characterized in that: The guide member is flexible; The pen body is provided with a drainage tube, the drainage member passes through the drainage tube and is connected to the printed drainage line, and the outlet of the drainage tube is arranged at an interval from the printed drainage line and is arranged on one side of the plane where the working track is located and close to the pen body.
10. The immunohistochemical pen according to claim 1, wherein The other end of the pen body is provided with an annular pen tip.