Cell culture plate with grooves in orifices
By designing grooves in different directions on the cell culture plate, the problem of adding wrong holes is solved, achieving higher operational accuracy and experimental success rate.
Patent Information
- Application Number
- CN202421854559.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-01
- Publication Date
- 2025-08-08
- Estimated Expiration
- 2034-08-01
AI Technical Summary
When adding liquid to the wells of a 24-well or above cell culture plate in the laboratory, it is easy to add wrong holes.
Design a cell culture plate with grooves on the orifice. The groove directions on any two adjacent culture holes are different. The groove shape is suitable for the placement of the pipette tip. The dosing is repeated in turn through wrist movements to indicate the dosing position.
Reduce the chance of adding wrong holes, reduce the mental pressure of operation, and improve the success rate of experiments.
Smart Images
Figure CN223201824U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a cell culture plate with an orifice provided with a groove, belonging to the technical field of cell culture. Background Art
[0002] A cell culture plate is a biological tool that can be divided into flat-bottom and round-bottom according to the shape of the bottom. The number of holes is available in different specifications, such as 6, 12, 24, 48, 96, and 384. Choose the appropriate cell culture plate according to the purpose of the experiment. For example, a 96-well flat-bottom plate is often chosen when using the MTT method for drug cytotoxicity experiments. Scientific researchers will add culture medium, cell suspension, drugs, etc. to each well in turn. These operations must be completed in the sterile environment of a biosafety cabinet. In actual applications, when experimenters use pipettes to repeatedly draw liquids, they need to maintain a high degree of concentration. While ensuring sterile operation, they must always pay attention to the sample addition position and are always affected by light. Especially for cell culture plates with 24 holes or more, the actual operation process is more labor-intensive and can easily cause problems with repeated addition of liquid to a certain well. Although pipettes can save effort and reduce errors, most laboratories do not have such conditions. Summary of the Invention
[0003] The technical problem to be solved by the utility model is: how to avoid adding liquid into the wrong wells when adding liquid into the wells of a cell culture plate with 24 wells or more in a laboratory.
[0004] In order to solve the above technical problems, the technical solution of the present invention is to provide a cell culture plate with a groove at the hole, including a culture plate main body and a plate cover matching the culture plate main body. The culture plate main body is provided with multiple culture wells, and is characterized in that a groove is provided around each of the culture wells, and the positions of the grooves on any two adjacent culture wells are different.
[0005] Preferably, each of the culture wells has grooves in different directions on the top, bottom, left and right sides; the shape of the groove can adapt to the placement of the pipette tip.
[0006] Preferably, according to the different positions of the grooves, the culture wells are divided into two types, one is a culture well with a groove at the three o'clock direction; the other is a culture well with a groove at the two o'clock direction.
[0007] Preferably, the grooves on the culture wells are all semicircular in shape.
[0008] Preferably, the culture plate body comprises a base and a top plate, and the base and the top plate are fixedly connected; and anti-corner features are provided on the top plate and the plate cover.
[0009] Preferably, the plate cover is a plate cover that can be movably assembled and disassembled from the culture plate body.
[0010] Compared with the prior art, the present invention has the following beneficial effects:
[0011] The utility model provides a cell culture plate with grooves at the orifices. Grooves in different directions are arranged on any two adjacent culture wells. When adding liquid to the culture wells, the experimenter can place the pipette tip with liquid on the groove in one direction of the culture well to drip the liquid, and place the pipette tip on the groove in the other direction of the next culture well to drip the liquid. The addition is circulated back and forth in sequence through wrist movement. The dripping of liquid in different directions can indicate the position of the last dripping well, which helps the experimenter reduce the probability of adding liquid to the wrong well, relieves the mental stress during operation, and thus improves the success rate of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] Figure 1 A top view of a cell culture plate (24 wells) with grooves at the wells.
[0013] Figure 2 A side view of the culture plate body. DETAILED DESCRIPTION
[0014] In order to make the present invention more clear and easy to understand, preferred embodiments are described in detail below with reference to the accompanying drawings.
[0015] The utility model provides a cell culture plate with a groove at the orifice, such as Figure 1 As shown, it includes a culture plate body 1 and a removable plate cover 5. The culture plate body 1 is provided with a plurality of culture wells 2. Each culture well 2 is surrounded by a groove. The grooves of any two adjacent culture wells 2 are positioned differently. Each culture well 2 has grooves in different directions, top, bottom, left, and right. The grooves can accommodate the placement of pipette tips. When in use, the plate cover 5 can be matched to cover the culture plate body 1.
[0016] In this embodiment, according to the different positions of the grooves, the culture holes 2 are divided into two types. One is a culture hole 2 with a groove at the three o'clock direction, and the groove on the culture hole 2 at this position is the first groove 3; the other is a culture hole 2 with a groove at the two o'clock direction, and the groove on the culture hole 2 at this position is the first groove 3.
[0017] The number of culture wells 2 of the cell culture plate in this embodiment is 24, and they are neatly arranged on the culture plate body 1. The grooves are arranged at the three o'clock and two o'clock directions of the culture wells 2, and are staggered in sequence. The grooves on the culture wells 2 (the first groove 3 and the first groove 3) are both semicircular in shape, which can accommodate the placement of 10μ and 100μ pipette tips.
[0018] like Figure 2As shown, the culture plate body 1 includes a base 12 and a top plate 11, the base 12 is fixedly connected to the top plate 11, and the top plate 12 and the plate cover 5 are provided with an anti-stupid corner 13, which is provided at the upper left corner of the top plate 12 and the plate cover 5, and the angle of the anti-stupid corner 13 is 45°.
[0019] The use process of this utility model is as follows:
[0020] When it is necessary to add drugs to the cells in each culture well 2 of the cell culture plate, the experimenter places the pipette tip with drug liquid on the first groove 3 at the three o'clock direction of the culture well 2 and drops the drug into the culture liquid. For the next culture well 2, the pipette tip is placed on the second groove 4 at the two o'clock direction and drops the drug. The drug is added back and forth in sequence through wrist movements. Adding drugs in different directions can indicate the position of the culture well 2 where the drug was added last, helping the experimenter reduce the chance of adding drugs to the wrong well, reducing the mental stress during operation, and thus improving the success rate of the experiment.
Claims
1. A cell culture plate with a grooved opening, comprising a culture plate body (1) and a plate cover (5) matching the culture plate body (1), wherein the culture plate body (1) is provided with a plurality of culture wells (2), characterized in that: A groove is provided around each of the culture wells (2), and the positions of the grooves on any two adjacent culture wells (2) are different.
2. A cell culture plate with grooves at the orifice according to claim 1, characterized in that: Each of the culture wells (2) has grooves in different directions on the top, bottom, left, and right sides; the shape of the grooves can adapt to the placement of a pipette tip.
3. The cell culture plate with grooves at the orifices according to claim 1, wherein: According to the different positions of the grooves, the culture wells (2) are divided into two types: one is a culture well (2) with a groove at the three o'clock direction; the other is a culture well (2) with a groove at the two o'clock direction.
4. A cell culture plate with grooves at the orifices according to claim 1, 2 or 3, characterized in that: The grooves on the culture holes (2) are all in the shape of a semicircular structure.
5. The cell culture plate with grooves at the orifices according to claim 1, wherein: The culture plate body (1) comprises a base (12) and a top plate (11), and the base (12) and the top plate (11) are fixedly connected; and anti-slip corners (13) are provided on the top plate (11) and the plate cover (5).
6. The cell culture plate with grooves at the orifices according to claim 1, wherein: The plate cover (5) is a plate cover (5) that can be movably assembled and disassembled from the culture plate main body (1).