Sterile uniform mixing and dropwise adding device

Abstract

The utility model discloses a sterile uniform mixing and dripping device which comprises a base, a rotating mechanism, a placing table, a circular ring, a supporting rod, a fixed arm, a clamping ring, a container and a valve, the placing table is rotatably connected to the base through a rotating mechanism; the ring is arranged on the upper surface of the placing table and is used for fixing a shake flask; the supporting rod is fixed on the placing table; one end of the support rod away from the placing table is connected with the first end of the fixed arm; a clamping ring is fixed to the second end of the fixing arm and located above the circular ring. A container is placed in the clamping ring, and a valve is arranged at a liquid outlet of the container. According to the scheme, through the arrangement of the rotating mechanism, a sterile experiment that transfection is independently operated by one person is achieved, and manpower resources are saved; and the device is small in size and convenient to transfer, and can be put into an ultra-clean workbench for operation after being disinfected, so that the sterility and the safety of the experiment are further ensured.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to an aseptic mixing and dropping device. Background Art

[0002] Currently, living materials in the Chinese market are widely used in life science research, biotechnology and medicine.

[0003] Especially when it comes to insect cell transfection or inoculation, especially the transient transfection process, the requirements are higher and the transfection reagent needs to be added dropwise while mixing. However, the cells to be transfected are mostly cultured in shake flasks. The entire operation process needs to be carried out aseptically. Two people are required to work together in an ultra-clean workbench, one to rotate the shake flask and the other to add the transfection reagent. The operation process is long, and the actual use process is not convenient and it is not easy to mix. Utility Model Content

[0004] In view of this, the utility model provides a sterile mixing and dropping device, which enables one person to independently operate a sterile transfection experiment, saving human resources. At the same time, the device is small in size and easy to transfer, which further ensures the sterility and safety of the experiment.

[0005] To achieve the above objectives, the present invention provides the following technical solutions:

[0006] An aseptic mixing and dropping device comprises: a base, a rotating mechanism, a placement table, a ring, a support rod, a fixed arm, a clamping ring, a container and a valve;

[0007] The placement table is rotatably connected to the base via the rotation mechanism;

[0008] The circular ring is arranged on the upper surface of the placement table, and the circular ring is used to fix the shaking bottle;

[0009] The support rod is fixed on the placement table; one end of the support rod away from the placement table is connected to the first end of the fixed arm;

[0010] The second end of the fixed arm is fixed with the snap ring, and the snap ring is located above the circular ring;

[0011] The container is placed in the clamping ring, and the valve is provided at the liquid outlet of the container.

[0012] Preferably, the number of the rotating mechanisms is multiple groups;

[0013] A plurality of sets of the rotating mechanisms are staggered and arranged between the base and the placement table;

[0014] Each of the rotating mechanisms comprises: a first bearing, a second bearing and a connecting rod;

[0015] The inner ring and outer ring of the first bearing are both fixedly connected to the lower surface of the placement table, and the inner ring and outer ring of the second bearing are both fixed to the upper surface of the base;

[0016] The first end of the connecting rod is fixedly connected to the ball of the first bearing, and the second end is fixedly connected to the ball of the second bearing.

[0017] Preferably, the number of the rotating mechanisms is four;

[0018] The base has four corners, the placement platform has four corners, and the four corners of the base and the four corners of the placement platform are respectively arranged opposite to each other;

[0019] Each set of the rotating mechanisms is arranged between the corners of the base and the corners of the placement platform that are arranged opposite to each other.

[0020] Preferably, projections of the axis of the first bearing and the axis of the second bearing in the vertical direction are staggered with each other.

[0021] Preferably, the number of the rotating mechanisms is multiple groups;

[0022] A plurality of sets of the rotating mechanisms are staggered and arranged between the base and the placement table;

[0023] Each of the rotating mechanisms comprises: a first bearing, a second bearing and a connecting rod;

[0024] The inner ring of the first bearing is fixedly connected to the lower surface of the placement table, and the inner ring of the second bearing is fixed to the upper surface of the base;

[0025] The first end of the connecting rod is fixedly connected to the outer circumferential wall of the first bearing, and the second end of the connecting rod is fixedly connected to the outer circumferential wall of the second bearing.

[0026] Preferably, the number of the rings is multiple;

[0027] The plurality of rings are detachably nested from inside to outside in order of diameter from small to large, the outermost ring is fixed to the upper surface of the placement table, and the innermost ring is used to fix the shaking bottle.

[0028] Preferably, the outermost ring is fixed to the upper surface of the placement table by magic glue.

[0029] Preferably, it also includes: a hand-held ring;

[0030] One end of the hand-held ring is connected to the placement table, and the other end is connected to the base.

[0031] Preferably, the container is funnel-shaped as a whole;

[0032] The liquid outlet is provided on the funnel neck of the container, the top of the funnel body of the container is closed, and a liquid inlet is provided on the side wall of the funnel body;

[0033] And / or, the valve is a throttle valve.

[0034] It can be seen from the above technical solution that the aseptic mixing and dropping device provided by the utility model, through the setting of the rotating mechanism, realizes that one person can independently operate the aseptic transfection experiment, saving human resources; and the device is small in size and easy to transfer, and can be placed in an ultra-clean workbench for operation after disinfection, which further ensures the sterility and safety of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0036] Figure 1 A schematic diagram of the overall structure of the aseptic mixing and dropping device provided in an embodiment of the utility model;

[0037] Figure 2 A schematic diagram of the overall structure of an embodiment of the rotating mechanism provided by the present utility model;

[0038] Figure 3 A schematic diagram of the overall structure of another embodiment of the rotating mechanism provided by the embodiment of the utility model;

[0039] Figure 4 This is an axonometric view of the overall structure of the circular ring provided in an embodiment of the present utility model.

[0040] Among them, 1 is the base, 2 is the rotating mechanism, 21 is the first bearing, 22 is the second bearing, 3 is the placement table, 4 is the ring, 5 is the hand-held ring, 6 is the support rod, 7 is the fixed arm, 8 is the clamping ring, 9 is the container, and 10 is the valve. DETAILED DESCRIPTION

[0041] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0042] The present invention provides a sterile mixing and dropping device, such as Figure 1-Figure 4 As shown, it includes: a base 1, a rotating mechanism 2, a placement table 3, a ring 4, a support rod 6, a fixed arm 7, a clamping ring 8, a container 9 and a valve 10;

[0043] The placement platform 3 is rotatably connected to the base 1 through the rotating mechanism 2;

[0044] The ring 4 is provided on the upper surface of the placement table 3 and is used to fix the shake bottle;

[0045] The support rod 6 is fixed on the placement platform 3; one end of the support rod 6 away from the placement platform 3 is connected to the first end of the fixed arm 7;

[0046] The second end of the fixed arm 7 is fixed with the snap ring 8, and the snap ring 8 is located above the circular ring 4;

[0047] The container 9 is placed in the clamping ring 8 , and the valve 10 is provided at the liquid outlet of the container 9 .

[0048] In the above technical solution, when in use, the device is first sterilized by high-temperature dry heat at 190℃-250℃ for more than 6 hours, and then the device is placed on a clean workbench; the prepared transfection reagent is added to the container 9, and then the shake flask to be transfected is placed in the fixed ring 4. After opening the valve 10 on the container 9, during the process of the transfection reagent dripping from the container 9 into the shake flask, a force is applied to the placement table 3, so that the placement table 3 rotates relative to the base 1 using the rotating mechanism 2, thereby driving the shake flask in the ring 4 to rotate until the transfection reagent in the shake flask is mixed and added. Then, the valve 10 is closed, and the next shake flask is replaced for transfection. The operation is repeated until all the transfection reagents are mixed, and then the device is cleaned and sterilized at high temperature before the next use, and then it can be reused to save resources.

[0049] Compared with existing technologies, this aseptic mixing and dropping device, through the setting of a rotating mechanism, enables one person to independently operate a sterile transfection experiment, saving human resources; the device is small in size and easy to transfer, and can be placed in an ultra-clean workbench for operation after disinfection, further ensuring the sterility and safety of the experiment; at the same time, it avoids the risk of transfection reagent spilling when mixing and dropping are carried out simultaneously.

[0050] Preferably, when in use, the device is first sterilized by high-temperature dry heat at 190°C-250°C for more than 6 hours, and then the device is placed in a clean workbench for assembly (it can be understood that the connection between the various components of this aseptic mixing and dropping device is a detachable connection); and after use, the various components are disassembled for cleaning and then sterilized at high temperature before the next use and can be reused; it should also be noted that the material of the base 1 is magnetic, the adsorption stability is better, and it is better fixed in the operating space.

[0051] Optimize the above technical solutions, such as Figure 2 As shown, the number of the rotating mechanisms 2 is multiple groups;

[0052] A plurality of sets of the rotating mechanisms 2 are staggered and arranged between the base 1 and the placement platform 3;

[0053] Each rotating mechanism 2 includes: a first bearing 21, a second bearing 22 and a connecting rod 23;

[0054] The inner ring and outer ring of the first bearing 21 are fixedly connected to the lower surface of the placement table 3, and the inner ring and outer ring of the second bearing 22 are fixed to the upper surface of the base 1;

[0055] A first end of the connecting rod 23 is fixedly connected to the ball of the first bearing 21 , and a second end of the connecting rod 23 is fixedly connected to the ball of the second bearing 22 .

[0056] In the above technical solution, the specific process of the placement table 3 rotating relative to the base 1 using the rotation mechanism 2 is: applying a rotational force to the placement table 3 (the tangential direction of the force can be clockwise or counterclockwise), and then using the connecting rod 23 and the ball, the placement table 3 rotates relative to the base 1; preferably, the ball in the second bearing 22 and the outer ring or inner ring of the second bearing 22 are fixed, so that the placement table 3 rotates relative to the connecting rod 23 (it can be understood that the first end of the connecting rod 23 serves as a fulcrum, and the placement table 3 rotates around the fulcrum); it should be noted that the above rotation amplitude range is 5-50mm, which is suitable for a variety of experimental needs.

[0057] Further optimization of the above technical solution, such as Figure 1 As shown, the number of the rotating mechanisms 2 is four;

[0058] The base 1 has four corners, and the placement platform 3 has four corners. The four corners of the base 1 and the four corners of the placement platform 3 are respectively arranged opposite to each other.

[0059] Each set of the rotating mechanisms 2 is arranged between the corners of the base 1 and the corners of the placement platform 3 that are arranged opposite to each other.

[0060] In the above technical solution, four sets of rotating components are respectively arranged at the four corners of the aseptic mixing and dropping device, which is beneficial to the stability of the structure and makes the placement table 3 rotate more smoothly relative to the base 1.

[0061] Further optimization of the above technical solution, such as Figure 1 As shown, the projections of the axis of the first bearing 21 and the axis of the second bearing 22 in the vertical direction are offset from each other.

[0062] In the above technical solution, such an arrangement enables the placement table 3 to rotate more smoothly relative to the base 1; preferably, the projections of part of the outer wall of the outer ring of the first bearing 21 and part of the outer wall of the outer ring of the second bearing 22 in the vertical direction are tangent.

[0063] In this technical solution, if Figure 1 and Figure 3 As shown, the number of the rotating mechanisms 2 is multiple groups;

[0064] A plurality of sets of the rotating mechanisms 2 are staggered and arranged between the base 1 and the placement platform 3;

[0065] Each rotating mechanism 2 includes: a first bearing 21, a second bearing 22 and a connecting rod 23;

[0066] The inner ring of the first bearing 21 is fixedly connected to the lower surface of the placement table 3, and the inner ring of the second bearing 22 is fixed to the upper surface of the base 1;

[0067] A first end of the connecting rod 23 is fixedly connected to the outer circumferential wall of the first bearing 21 , and a second end of the connecting rod 23 is fixedly connected to the outer circumferential wall of the second bearing 22 .

[0068] In the above technical solution, the outer ring of the first bearing 21 rotates relative to the inner ring, and the outer ring of the second bearing 22 rotates relative to the inner ring, and then the connecting rod 23 is used to realize the rotation of the placement table 3 relative to the base 1; in one technical solution, the outer ring of the second bearing 22 is also fixed to the upper surface of the base 1, and the outer ring of the first bearing 21 is rotated relative to the inner ring to drive the connecting rod 23 to rotate, thereby realizing the rotation of the placement table 3 relative to the base 1; in another technical solution, the outer ring of the first bearing 21 is fixed to the lower surface of the placement table 3, and the outer ring of the second bearing 22 is rotated relative to the inner ring to drive the connecting rod 23 to rotate, thereby realizing the rotation of the placement table 3 relative to the base 1.

[0069] In this technical solution, if Figure 1 and Figure 4 As shown, the number of the rings 4 is multiple;

[0070] The plurality of rings 4 are detachably nested from the inside to the outside and arranged in increasing diameters. The outermost ring 4 is fixed to the upper surface of the placement table 3 , and the innermost ring 4 is used to fix the shaking bottle.

[0071] In the above technical solution, different types of shake bottles can be adapted by increasing or decreasing the number of rings 4; preferably, the radial thicknesses of the rings 4 are different, and rings 4 of different thicknesses are adapted to different types of shake bottles; it should be noted that different combinations of rings 4 can match 125ml-5L shake bottles.

[0072] To optimize the above technical solution, the outermost ring 4 is fixed to the upper surface of the placement table 3 by magic glue.

[0073] In the above technical solution, the outermost ring 4 is bonded to the placement table 3 by magic glue, which can improve the assembly efficiency of the entire device.

[0074] In this technical solution, if Figure 1 As shown, the fixed arm 7 is rotatably connected to the support rod 6. Since a clamping ring 8 is fixed on the fixed arm 7, the fixed arm 7 can be rotated horizontally relative to the support rod 6 according to the different positions of the remote bottle on the placement table 3, so that the axial direction of the remote bottle and the axial direction of the liquid outlet of the container 9 in the clamping ring 8 always coincide with each other. This arrangement avoids the transfection reagent from being easily dripped onto the wall of the shaking flask and spilled during the operation process, affecting the accuracy of the sample addition, resulting in poor experimental repeatability and contamination.

[0075] In addition, the fixed arm 7 can also rotate in the vertical direction relative to the support rod 6 to adjust the vertical distance between the remote bottle and the liquid outlet of the container 9 in the clamping ring 8.

[0076] In this technical solution, if Figure 1 As shown, it also includes: a hand-held ring 5 for convenient manual mixing;

[0077] One end of the hand-held ring 5 is connected to the placement table 3 , and the other end is connected to the base 1 .

[0078] In the above technical solution, the operator uses the handheld ring 5 to save time and effort to rotate the placement table 3 relative to the base 1; it should be noted that the experimental process only requires the operator to mix through the handheld ring 5, and the amplitude is controllable to avoid the generation of a large number of bubbles that may cause irreversible damage to the cells.

[0079] like Figure 1 As shown, the container 9 is funnel-shaped as a whole;

[0080] The funnel neck of the container 9 is provided with a liquid outlet, the top of the funnel body of the container 9 is closed, and a liquid inlet is provided on the side wall of the funnel body;

[0081] And / or, the valve 10 is a throttle valve for adjusting the dripping speed, so that the dripping flow rate is controllable and adjustable, the operation is more convenient, and the experimenter is prevented from arm pain caused by holding the sample gun for a long time.

[0082] In the above technical solution, a liquid inlet is provided on the side wall to prevent bacteria from falling into the container and contaminating the sample when objects pass from above. In addition, precise scale lines are marked on the container 9 to facilitate real-time observation of the amount added. The container 9 is made of glass and can withstand high-temperature dry heat sterilization above 190°C. High-temperature dry heat sterilization above 190°C avoids the risk of cell contamination caused by consumables. It should also be noted that the transfection reagent is added to the container 9 using an electric pipette.

[0083] The technical features mentioned above and those shown individually in the accompanying drawings may be combined arbitrarily, provided that the combined technical features do not conflict with each other. All possible feature combinations are technical contents explicitly described herein. Any of the multiple sub-features included in the same statement may be applied independently, and does not necessarily have to be applied in conjunction with the other sub-features.

[0084] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0085] The above description of the disclosed embodiments will enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A sterile mixing and dropping device, characterized in that: include: Base (1), rotating mechanism (2), placement platform (3), ring (4), support rod (6), fixed arm (7), snap ring (8), container (9) and valve (10); The placement platform (3) is rotatably connected to the base (1) via the rotating mechanism (2); The circular ring (4) is arranged on the upper surface of the placement table (3), and the circular ring (4) is used to fix the shaking bottle; The support rod (6) is fixed on the placement platform (3); one end of the support rod (6) away from the placement platform (3) is connected to the first end of the fixed arm (7); The second end of the fixed arm (7) is fixed with the snap ring (8), and the snap ring (8) is located above the circular ring (4); The container (9) is placed inside the clamping ring (8), and the valve (10) is provided at the liquid outlet of the container (9).

2. The aseptic mixing and dropping device according to claim 1, characterized in that: The number of the rotating mechanisms (2) is multiple; A plurality of sets of the rotating mechanisms (2) are staggered and arranged between the base (1) and the placement platform (3); Each of the rotating mechanisms (2) comprises: a first bearing (21), a second bearing (22) and a connecting rod (23); The inner ring and the outer ring of the first bearing (21) are both fixedly connected to the lower surface of the placement table (3), and the inner ring and the outer ring of the second bearing (22) are both fixed to the upper surface of the base (1); The first end of the connecting rod (23) is fixedly connected to the ball of the first bearing (21), and the second end is fixedly connected to the ball of the second bearing (22).

3. The aseptic mixing and dropping device according to claim 2, characterized in that: The number of the rotating mechanisms (2) is four; The base (1) has four corners, the placement platform (3) has four corners, and the four corners of the base (1) and the four corners of the placement platform (3) are respectively arranged opposite to each other. Each set of the rotating mechanisms (2) is arranged between the corners of the base (1) and the corners of the placement platform (3) that are arranged opposite to each other.

4. The aseptic mixing and dropping device according to claim 2, characterized in that: The projections of the axis of the first bearing (21) and the axis of the second bearing (22) in the vertical direction are staggered with each other.

5. The aseptic mixing and dropping device according to claim 1, characterized in that: The number of the rotating mechanisms (2) is multiple; A plurality of sets of the rotating mechanisms (2) are staggered and arranged between the base (1) and the placement platform (3); Each of the rotating mechanisms (2) comprises: a first bearing (21), a second bearing (22) and a connecting rod (23); The inner ring of the first bearing (21) is fixedly connected to the lower surface of the placement table (3), and the inner ring of the second bearing (22) is fixed to the upper surface of the base (1); The first end of the connecting rod (23) is fixedly connected to the outer circumferential wall of the first bearing (21), and the second end is fixedly connected to the outer circumferential wall of the second bearing (22).

6. The aseptic mixing and dropping device according to claim 1, characterized in that: The number of the rings (4) is multiple; The plurality of circular rings (4) are detachably nested from the inside to the outside in order of diameter from small to large, the outermost circular ring (4) is fixed to the upper surface of the placement table (3), and the innermost circular ring (4) is used to fix the shaking bottle.

7. The aseptic mixing and dropping device according to claim 6, characterized in that: The outermost ring (4) is fixed to the upper surface of the placement platform (3) by magic glue.

8. The aseptic mixing and dropping device according to claim 1, characterized in that: The fixed arm (7) is rotatably connected to the support rod (6).

9. The aseptic mixing and dropping device according to claim 1, characterized in that: Also includes: Hand-held ring (5); One end of the hand-held ring (5) is connected to the placement table (3), and the other end is connected to the base (1).

10. The aseptic mixing and dropping device according to any one of claims 1 to 9, characterized in that: The container (9) is funnel-shaped as a whole; The liquid outlet is provided on the funnel neck of the container (9), the top of the funnel body of the container (9) is closed, and a liquid inlet is provided on the side wall of the funnel body; And / or, the valve (10) is a throttle valve.

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