Multi-channel diamond array sample application piece

Abstract

The utility model discloses a multi-channel diamond array spotting piece, the spotting piece comprises a support arm, an objective table, a solenoid valve group, a plurality of ceramic needles and a spotting plate, one end of the support arm is connected with the rear end of the objective table, the other end is externally connected with a driving assembly of the spotting plate, the solenoid valve group is installed at the bottom of the objective table, and the ceramic needles are arranged on the ceramic needles. The electromagnetic valve group comprises a plurality of electromagnetic valves which are arranged in a staggered manner along the central axis direction of the objective table, the top end of each electromagnetic valve is respectively and independently clamped with a liquid inlet pipe, the bottom end of each electromagnetic valve is respectively connected with a ceramic needle, and the point sample plate is positioned under the objective table and moves unidirectionally along the central axis direction of the objective table according to a fixed step length. According to the sample application piece disclosed by the utility model, multi-channel sample application of a rhombus array, a regular triangle array or an inverted triangle array is completed on test paper at one time, the problem of position deviation is solved, and the sample application accuracy is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of spotting devices, in particular to a multi-channel diamond array spotting component. Background Art

[0002] When preparing immunochromatographic test strips, it is usually necessary to use a spotting device to pre-spray a micro-quantitative amount of a solution such as an antigen or antibody on the test strip to form a corresponding test line or test point. On a test strip containing multiple test points, the test points are usually arranged in a linear single row, an aligned array, or a diamond array. For the arrangement of linear single rows and aligned arrays, the existing linear multi-nozzle spotting device can achieve rapid spotting, such as patent CN202122552716.0, a multi-channel array spotting device for medical testing, in which the bottom of its liquid storage tank is evenly spaced and connected to multiple one-way valves, and the bottom of the one-way valve is fixedly connected to a liquid pipe, and then the spotting plate at the bottom is driven by a motor for lateral displacement, thereby achieving array spotting, to improve spotting efficiency. However, the nozzle of the above-mentioned spotting device is arranged in a linear single row, which can only be used for spotting in the arrangement of linear single rows and aligned arrays, and cannot meet the needs of dislocated diamond array spotting.

[0003] For diamond array spotting, a three-dimensional spotting device is mainly used. Specifically, the three-dimensional spotting device also contains multiple spotting channels, which control the movement of the spotting plate along the X, Y, and Z directions to achieve multi-channel spotting of different arrays. Taking the spotting of the diamond array as an example, after the spotting of the previous channel is completed, the machine needs to control the spotting plate to immediately undergo horizontal displacement according to the position of the next channel, and then proceed to spot the next channel. The whole process requires multiple reciprocating motions to achieve. Based on the characteristics of reciprocating motion, the spotting system itself cannot perfectly replicate the position coordinates of each reciprocating motion, and slight deviations are prone to occur; if the starting coordinates of each reciprocating motion are different, then the spotting device needs to overcome the system deviation while also making corresponding horizontal movements. The superposition of these two factors will further increase the position deviation. The test strips are usually made up of a flushing pad, a buffer pad, a reaction membrane and an absorbent pad, which are overlapped in sequence. The length is usually 3 to 10 cm and the width is only 0.8 to 1 cm. In particular, the width of the reaction membrane (i.e., the area where the sample needs to be spotted) is only 4 to 6 mm and the length is only 8 to 10 mm. Four different antigen or antibody solutions must be coated within this range, and the four coating points are arranged in a diamond shape. The range is small and the accuracy deviation is at the mm level, which makes the position deviation have a more obvious effect on the spotting effect. In addition, the reciprocating motion spotting also has the problems of many steps and low efficiency. Therefore, it is necessary to further improve the precision and efficiency of the diamond array spotting. Utility Model Content

[0004] In order to solve the above problems, the utility model provides a multi-channel diamond array spotting element.

[0005] The utility model adopts the following technical solutions:

[0006] A multi-channel diamond array spotting component comprises a support arm, a stage, a solenoid valve group, a plurality of ceramic needles and a spotting plate. One end of the support arm is connected to the rear end of the stage, and the other end is externally connected to a drive assembly of the spotting machine. The solenoid valve group is mounted at the bottom of the stage and contains a plurality of battery valves staggered along the central axis of the stage. The top of each battery valve is independently clamped with a liquid inlet tube, and the bottom of each battery valve is connected to a ceramic needle. The spotting plate is located directly below the stage and moves unidirectionally along the central axis of the stage at a fixed step size.

[0007] Furthermore, four channel holes are provided at the bottom of the loading platform, and the channel holes are staggered along the central axis of the loading platform.

[0008] Furthermore, the channel hole is a hollow cylindrical hole along the vertical direction.

[0009] Furthermore, the solenoid valve group contains four independently controlled one-way solenoid valves.

[0010] Furthermore, the inner diameter of the channel hole matches the outer diameter of the solenoid valve, and the battery valve is locked or clamped in the channel hole.

[0011] Furthermore, the step length of each movement of the spotting plate is 8 to 10 mm.

[0012] Furthermore, the channel holes are respectively a first channel hole, a second channel hole, a third channel hole and a fourth channel hole, the first channel hole and the fourth channel hole are both located on the central axis of the stage, and the second channel hole and the third channel hole are respectively located on the left and right side of the central axis.

[0013] Furthermore, the second channel hole and the third channel hole are at an equal distance from the central axis, and the distance is 1 to 2 mm.

[0014] Furthermore, the distance between the first channel hole and the fourth channel hole is 5 to 7 mm greater than three times the step length.

[0015] After adopting the above technical solution, the utility model has the following advantages compared with the background technology:

[0016] 1. The spotting component of the present invention is equipped with four staggered solenoid valves that independently control the liquid spraying and are connected to different liquid inlet tubes. Each liquid inlet tube contains a different antigen or antibody coating solution, thus forming a four-channel spotting component. As the spotting plate on the spotting machine table moves along the central axis, multi-channel diamond array spotting can be performed on the test strip. In addition, by independently controlling the connection of different liquid inlet tube combinations through battery valves, multi-channel spotting of regular triangle arrays or inverted triangle arrays can also be performed, overcoming the defect that existing linear multi-nozzle spotting devices cannot meet the requirements of staggered diamond array spotting.

[0017] 2. The spotting device of the present invention is configured with channel holes staggered along the central axis on the stage, and then equipped with a battery valve and a ceramic needle, thereby staggering the spotting points on the diamond array, increasing the distance between them and avoiding mutual influence between the spotting points. Combined with the same-frequency movement of the spotting plate, multi-channel spotting of a diamond array, an equilateral triangle array, or an inverted triangle array can be completed on the test paper at one time, thereby improving the problem of position deviation and enhancing the accuracy of spotting.

[0018] 3. The position of the spot formed by the spotting element in the spotting area is relatively fixed, and there is no need to adjust the position of the battery valve and the ceramic needle. The sample can be spotted at a fixed position on the test strip. The operation is simple. The spotting plate only needs to move in a single direction along the central axis. Unlike the existing three-dimensional spotting device, which requires controlling the spotting plate to move in the X, Y, and Z directions, the reciprocating motion spotting method overcomes the problems of multiple steps and low efficiency, and effectively improves the spotting efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of the sample point of the utility model;

[0020] Figure 2 Schematic diagram of the arrangement of channel holes of the sample spotting component of the present invention;

[0021] Figure 3 This is a schematic diagram of the process of using the spotting piece of the utility model for spotting diamond arrays.

[0022] Description of reference numerals:

[0023] 1. Support arm;

[0024] 2. Loading platform;

[0025] 3. Battery valve;

[0026] 4. Ceramic needle;

[0027] 5. Channel hole; 51. First channel hole; 52. Second channel hole; 53. Third channel hole; 54. Fourth channel hole;

[0028] 6. Click on the sample. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0030] Example

[0031] like Figure 1 As shown, a multi-channel diamond array spotting device includes a support arm 1, a stage 2, a solenoid valve assembly, several ceramic needles 4, and a spotting plate 6. One end of the support arm 1 is connected to the rear end of the stage 2, and the other end is connected to the drive assembly of the spotting machine. The drive assembly drives the stage 2 along the X, Y, and Z axes through the support arm 1. Four channel holes 5 are provided at the bottom of the stage 2, and the channel holes 5 are staggered along the central axis of the stage 2. The channel holes 5 are hollow cylindrical holes along the vertical direction.

[0032] The solenoid valve assembly contains four independently controlled one-way solenoid valves 3. The solenoid valve assembly is installed at the bottom of the stage 2. The inner diameter of the channel hole 5 matches the outer diameter of the solenoid valve 3, and the battery valve 3 is locked in the channel hole 5.

[0033] The solenoid valve group contains a plurality of battery valves 3 staggered along the central axis of the stage 2 , the top of each battery valve 3 is independently connected to a liquid inlet pipe, and the bottom of each battery valve 3 is connected to a ceramic needle 4 .

[0034] The spotting plate 6 is located directly below the stage 2, and the spotting plate 6 moves unidirectionally along the central axis of the stage 2 at a fixed step length and a fixed frequency, wherein the battery valve group and the spotting plate 6 have the same frequency, that is, the opening frequency of the battery valve group is the same as the movement frequency of the spotting plate 6. For example, the stage 2 is moved to a suitable position, that is, aligned with the spotting area, and then fixed under the drive component of the spotting machine, while the spotting plate 6 moves one step length along the central axis per second. The solenoid valve group is used to control the spotting frequency of the ceramic needle 4 to be the same as the movement frequency of the spotting plate 6, so that spotting can be completed on the spotting plate 6.

[0035] Specifically, in this embodiment, the step length of each movement of the sample plate 6 is 9 mm, which is the width of the test strip. The step length is determined based on the width of the test strip. This embodiment uses a test strip with a width of 9 mm and a length of 6 cm as an example. The test strips are arranged side by side on the sample plate 6, and the sample plate 6 drives the test strips to move horizontally. Therefore, the step length is the width of the test strip, 9 mm. Similarly, if the test strips are arranged end to end on the sample plate 6, the step length of each movement of the sample plate 6 is the length of the test strip.

[0036] In this embodiment, the staggered arrangement of the channel holes 5 is used to stagger the sampling points on the diamond array, thereby increasing the distance between the sampling points to avoid mutual influence between the sampling points. Combined with the movement of the sampling plate at the same frequency, multi-channel sampling of a diamond array, an equilateral triangle array, or an inverted triangle array can be completed on the test paper at one time, thereby improving the problem of position deviation and improving the accuracy of sampling.

[0037] Specifically, such as Figure 2 As shown, the channel holes 5 are respectively the first channel hole 51, the second channel hole 52, the third channel hole 53 and the fourth channel hole 54. The first channel hole 51 and the fourth channel hole 54 are both located on the central axis of the stage 2, and the second channel hole 52 and the third channel hole 53 are respectively located on the left and right sides of the central axis. The second channel hole 52 and the third channel hole 53 are equidistant from the central axis and the distance is 1.5 mm. The distance between the first channel hole 51 and the fourth channel hole 54 is 6 mm longer than the triple step length, i.e., 33 mm. In order to further understand the positional relationship between the four channel holes 5, we also Figure 2 The distances between the channel holes along the central axis are marked, such as: the distance between the first channel hole 51 and the second channel hole 52 along the central axis d1 = step length + 3mm = 12mm, the distance between the second channel hole 52 and the third channel hole 53 along the central axis d2 = step length = 9mm, and the distance between the third channel hole 53 and the fourth channel hole 54 along the central axis d3 = step length + 3mm = 12mm.

[0038] The spotting process is now explained using a diamond array as an example. The structure and driving principle of the spotting machine are prior art and will not be described in detail here. For details, please refer to the description in patent CN 202122552716.0, "A Multi-channel Array Spotting Device for Medical Testing," or the working principle of existing three-dimensional spotting machines. This embodiment only illustrates the working principle of the spotting device of the present invention. The details are as follows:

[0039] In the sample spotting part of the embodiment, 4 electromagnetic valves 3 for independently controlling the liquid spray are configured and connected to the liquid inlet pipe. The liquid in each liquid inlet pipe can be a different antigen or antibody coating solution. Figure 3As shown, when the spotting machine is running, 4 channels start to spray at the same time (assuming the spraying rate is 1 drop / second), and each channel corresponds to a different diamond vertex on a unit of test strip, so that the spraying of different solutions on four test strips can be completed at the first second; then, the spotting plate 6 moves the length of the test strip; at the second second, the solution continues to spray the second coating solution on the test strip that has been coated with one solution; then, the spotting plate 6 moves the width of the test strip; at the third second, the solution continues to spray the third coating solution on the test strip that has been coated with two solutions; then, the spotting plate 6 moves the length of the test strip; at the fourth second, the solution continues to spray the fourth coating solution on the test strip that has been coated with three solutions. According to the above spraying rules, the detection points of the diamond needle array can be obtained on a test strip at the fourth second of the spotting operation (see Figure 3 After one spotting step, a spotting plate with a diamond array of detection points can be obtained.

[0040] By independently controlling the battery valve 3 to connect different liquid inlet pipe combinations, multi-channel spotting of a regular triangle array or an inverted triangle array can be performed, overcoming the defect that the existing linear multi-nozzle spotting device cannot meet the requirements of staggered diamond array spotting.

[0041] The spotting device of the present invention can be used for multi-channel spotting of a diamond array, an equilateral triangle array, or an inverted triangle array. The positions of the dots formed in the spotting area are relatively fixed, and there is no need to adjust the positions of the battery valve 3 and the ceramic needle 4. The spotting can be performed at a fixed position on the test strip, and the operation is simple. The spotting plate 6 only needs to move in a single direction along the central axis, unlike the existing three-dimensional spotting device which requires controlling the spotting plate 6 to move in the X, Y, and Z directions. This overcomes the problems of reciprocating motion spotting with multiple steps and low efficiency, and effectively improves the spotting efficiency.

[0042] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.

Claims

1. A multi-channel diamond array spotting device, characterized by: It includes a support arm, a stage, a solenoid valve group, several ceramic needles and a spotting plate. One end of the support arm is connected to the rear end of the stage, and the other end is connected to the driving component of the spotting machine. The solenoid valve group is installed at the bottom of the stage. The solenoid valve group contains several battery valves staggered along the central axis of the stage. The top of each battery valve is independently clamped with a liquid inlet pipe, and the bottom of each battery valve is connected to a ceramic needle. The spotting plate is located directly below the stage, and the spotting plate moves unidirectionally along the central axis of the stage at a fixed step size.

2. The multi-channel diamond array spotting element according to claim 1, characterized in that: Four channel holes are provided at the bottom of the loading platform, and the channel holes are staggered along the central axis of the loading platform.

3. The multi-channel diamond array spotting element according to claim 2, characterized in that: The channel hole is a hollow cylindrical hole along the vertical direction.

4. The multi-channel diamond array spotting element according to claim 3, characterized in that: The solenoid valve group contains four independently controlled one-way solenoid valves.

5. The multi-channel diamond array spotting element according to claim 4, characterized in that: The inner diameter of the channel hole matches the outer diameter of the solenoid valve, and the battery valve is locked or clamped in the channel hole.

6. The multi-channel diamond array spotting element according to claim 5, characterized in that: The step length of each movement of the spotting plate is 8 to 10 mm.

7. The multi-channel diamond array spotting element according to claim 6, characterized in that: The channel holes are respectively the first channel hole, the second channel hole, the third channel hole and the fourth channel hole. The first channel hole and the fourth channel hole are both located on the central axis of the stage, and the second channel hole and the third channel hole are respectively located on the left and right side of the central axis.

8. The multi-channel diamond array spotting element according to claim 7, characterized in that: The second channel hole and the third channel hole are at the same distance from the central axis, and the distance is 1-2 mm.

9. The multi-channel diamond array spotting element according to claim 8, characterized in that: The distance between the first channel hole and the fourth channel hole is 5 to 7 mm greater than the triple step length.

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