Vacuum filtration device

Abstract

The utility model discloses a vacuum filtration device, and relates to the technical field of cell preparation, the vacuum filtration device comprises a first funnel assembly, a second funnel assembly and a reciprocating vacuum pump, the first funnel assembly and the second funnel assembly respectively comprise a funnel body and a cover body, and the cover body detachably covers the funnel body; the funnel body of the first funnel assembly is detachably connected with the cover body of the second funnel assembly; the funnel body of the first funnel assembly and the funnel body of the second funnel assembly are detachably connected with the air inlet end of the reciprocating vacuum pump; the air outlet end of the reciprocating vacuum pump is detachably connected with the funnel body of the first funnel assembly; the problems of low filtering efficiency and inconvenience in cleaning are solved; the whole structure is convenient to disassemble, assemble and sterilize, potential problems caused by liquid suck-back in the filtering process are avoided, negative pressure is provided through the reciprocating vacuum pump, meanwhile, pressurization is conducted in the funnel body of the first funnel assembly, and the filtering efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the field of cell preparation technology, in particular to a vacuum filtration device. Background Art

[0002] A vacuum filtration device suitable for filtering cell suspensions during automated cell preparation cycles. Filtration uses a vacuum pump to reduce the pressure in a filtration flask to achieve solid-liquid separation. Vacuum filtration accelerates filtration by utilizing the negative pressure created by the pumped air. A collection flask is connected to a vacuum pump, which reduces the air pressure within the flask. Under the influence of atmospheric pressure and gravity, the solution passes through the filter cloth / mesh and flows into the collection flask, while any remaining material remains on the filter paper, achieving the desired filtration effect.

[0003] In the research fields of biology, medicine, etc., the filtered materials are different from the common solid materials visible to the naked eye. They include cells / cell ruptures, proteins, cytoskeleton, etc. Sometimes, it is also necessary to consider the cell culture cycle and the problems caused by the viscosity of the cell suspension due to excessive cell growth density. At present, in actual artificial applications, for cell culture, filtration after centrifugal collection still mainly uses manual injection filtration, which is inefficient and time-consuming, has a potential impact on cell quality, and to a certain extent, there is a risk of contamination of cells. The filtration devices in the existing technology have technical problems such as low filtration efficiency and inconvenient cleaning. Therefore, in response to this situation, there is an urgent need to develop a vacuum filtration device to meet the needs of actual use. Utility Model Content

[0004] In view of this, the present invention aims to address the deficiencies in the existing technology, and its main purpose is to provide a vacuum filtration device, which solves the problems of low efficiency and inconvenient cleaning in the existing filtration process; the overall structure is convenient for disassembly, assembly and sterilization, provides a closed filtration environment, and avoids the potential problems caused by liquid backflow during the filtration process. Negative pressure is provided by a reciprocating vacuum pump, and the funnel body of the first funnel assembly is pressurized at the same time, thereby improving the filtration efficiency.

[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0006] A vacuum filtration device includes a first funnel assembly, a second funnel assembly and a reciprocating vacuum pump, wherein the first funnel assembly and the second funnel assembly each include a funnel body and a cover body, wherein the cover body is detachably covered on the funnel body; the funnel body of the first funnel assembly and the cover body of the second funnel assembly are detachably connected; the funnel body of the first funnel assembly and the funnel body of the second funnel assembly are both detachably connected to the air inlet end of the reciprocating vacuum pump; and the air outlet end of the reciprocating vacuum pump is detachably connected to the funnel body of the first funnel assembly.

[0007] As a preferred solution: the vacuum filtration device also includes a first air inlet pipe, a second air inlet pipe and an air outlet pipe, one end of the first air inlet pipe is connected to the lower side of the funnel body of the first funnel assembly, one end of the second air inlet pipe is connected to the lower side of the funnel body of the second funnel assembly, the other end of the first air inlet pipe and the other end of the second air inlet pipe are both connected to the air inlet end of the reciprocating vacuum pump; one end of the air outlet pipe is connected to the air outlet end of the reciprocating vacuum pump, and the other end of the air outlet pipe is connected to the upper side of the funnel body of the first funnel assembly.

[0008] As a preferred solution: the funnel body includes a funnel portion located on the upper side and a liquid outlet portion located on the lower side, the funnel portion has a liquid storage cavity for accommodating the liquid to be filtered, and the lower end of the liquid storage cavity is provided with a placement plane for placing filter paper.

[0009] As a preferred solution: the liquid outlet includes a guide tube and a nozzle tube, the guide tube is sleeved on the outside of the nozzle tube, the lower end of the nozzle tube is inclined, and the guide tube extends downward beyond the lower end of the nozzle tube.

[0010] As a preferred solution: a vent is provided on the outer wall of the guide tube, the reciprocating vacuum pump has an air inlet and an air outlet, the air inlet end of the reciprocating vacuum pump is the air inlet, the air outlet end of the reciprocating vacuum pump is the air outlet, and the air inlet is connected to the vent.

[0011] As a preferred solution: an internal thread is provided in the inner wall of the lower end of the guide tube, and the internal thread is located below the nozzle; a connecting tube is provided on the cover body of the second funnel assembly, and an external thread matching the internal thread is provided on the upper end of the connecting tube; the funnel body of the first funnel assembly and the cover body of the second funnel assembly are detachably connected through the cooperation of the internal thread and the external thread.

[0012] As a preferred solution: the cover of the first funnel assembly is provided with an opening to prevent excessive negative pressure on the funnel body, and the opening is provided on the upper surface of the cover.

[0013] Compared with the prior art, the present invention has obvious advantages and beneficial effects. Specifically, it can be seen from the above technical scheme that the vacuum filtration device provided by the present application improves the cell filtration efficiency, is easy to operate, can be applied to high-temperature and high-pressure sterilization, and has a wide range of applications; it solves the problems of low efficiency and inconvenient cleaning in the existing filtration process; the overall structure is convenient for disassembly, assembly and sterilization, provides a closed filtration environment, and avoids the potential problems caused by liquid backflow during the filtration process. Negative pressure is provided by a reciprocating vacuum pump, and the funnel body of the first funnel assembly is pressurized at the same time, thereby improving the filtration efficiency.

[0014] In order to more clearly illustrate the structural features and effects of the present invention, it is described in detail below with reference to the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the three-dimensional structure of a vacuum filtration device from the first perspective of the present invention;

[0016] Figure 2 This is a schematic diagram of the three-dimensional structure of a vacuum filtration device according to the present invention from a second perspective;

[0017] Figure 3 This is a schematic diagram of the three-dimensional structure of a vacuum filtration device according to the present invention from a third perspective;

[0018] Figure 4 This is a schematic diagram of the third perspective structure of a vacuum filtration device of the present invention.

[0019] Description of the accompanying drawings:

[0020] In the figure: 10, first funnel assembly; 20, second funnel assembly; 21, funnel body; 211, funnel part; 212, liquid storage chamber; 213, placement plane; 214, liquid outlet; 215, guide tube; 216, vent; 217, internal thread; 218, nozzle; 22, cover body; 221, connecting tube; 222, external thread; 223, opening; 30, reciprocating vacuum pump; 40, first air inlet pipe; 50, second air inlet pipe; 60, air outlet pipe. DETAILED DESCRIPTION

[0021] The utility model Figures 1 to 4 As shown, a vacuum filtration device includes a first funnel assembly 10, a second funnel assembly 20 and a reciprocating vacuum pump 30, wherein:

[0022] The first funnel assembly 10 and the second funnel assembly 20 both include a funnel body 21 and a cover body 22, and the cover body 22 is detachably covered on the funnel body 21; the funnel body 21 of the first funnel assembly 10 is detachably connected to the cover body 22 of the second funnel assembly 20; the funnel body 21 of the first funnel assembly 10 and the funnel body 21 of the second funnel assembly 20 are both detachably connected to the air inlet end of the reciprocating vacuum pump 30; the air outlet end of the reciprocating vacuum pump 30 is detachably connected to the funnel body 21 of the first funnel assembly 10.

[0023] The funnel body 21 of the second funnel assembly 20 is connected to a collection bottle for collecting the filtrate; when in use, the first funnel assembly 10, the second funnel assembly 20 and the collection bottle are fixed on a fixing frame, the cell suspension is poured into the funnel body 21 of the first funnel assembly 10, and the reciprocating vacuum pump 30 is started. The lower side of the funnel body 21 forms a negative pressure, and the cell suspension flows toward the collection bottle under the action of the air pressure, wherein the liquid flows into the collection bottle through the filter cloth / filter screen, and the macromolecular substances such as cell rupture products and cytoskeleton are filtered out. Filter cloth / filter screen; while the reciprocating vacuum pump 30 draws vacuum to form negative pressure on the lower side of the funnel body 21, gas will be input into the funnel body 21 of the first funnel assembly 10 through the air outlet end of the reciprocating vacuum pump 30, further increasing the speed at which the mixed liquid flows toward the collection bottle, thereby improving the filtration efficiency; in addition, the corresponding connections between the first funnel assembly 10, the second funnel assembly 20 and the reciprocating vacuum pump 30 adopt a detachable connection method, so when cleaning, they can be disassembled for cleaning, which is convenient for cleaning, disinfection and sterilization operations.

[0024] The vacuum filtration device provided in the present application improves the cell filtration efficiency and has the characteristics of easy operation. It can be applied to high-temperature and high-pressure sterilization and has a wide range of applications. It solves the problems of low efficiency and inconvenient cleaning in the existing filtration process. The overall structure is convenient for disassembly, assembly and sterilization, provides a closed filtration environment, and avoids the potential problems caused by liquid backflow during the filtration process. Negative pressure is provided by a reciprocating vacuum pump 30, and the funnel body 21 of the first funnel assembly 10 is pressurized at the same time, thereby improving the filtration efficiency.

[0025] The vacuum filtration device also includes a first air inlet pipe 40, a second air inlet pipe 50 and an air outlet pipe 60, one end of the first air inlet pipe 40 is connected to the lower side of the funnel body 21 of the first funnel assembly 10, one end of the second air inlet pipe 50 is connected to the lower side of the funnel body 21 of the second funnel assembly 20, the other end of the first air inlet pipe 40 and the other end of the second air inlet pipe 50 are both connected to the air inlet end of the reciprocating vacuum pump 30; one end of the air outlet pipe 60 is connected to the air outlet end of the reciprocating vacuum pump 30, and the other end of the air outlet pipe 60 is connected to the upper side of the funnel body 21 of the first funnel assembly 10.

[0026] By adopting the first air inlet pipe 40, the second air inlet pipe 50 and the air outlet pipe 60 to realize the air inlet and outlet of the reciprocating vacuum pump 30, negative pressure is generated on the lower side of the funnel body 21, and the air outlet pressure is increased on the upper side of the first funnel assembly 10, thereby improving the filtering efficiency.

[0027] The funnel body 21 includes a funnel portion 211 located at the upper side and a liquid outlet portion 214 located at the lower side. The funnel portion 211 has a liquid storage cavity 212 for accommodating liquid to be filtered. The lower end of the liquid storage cavity 212 is provided with a placement plane 213 for placing filter paper.

[0028] The filter paper or filter mesh is placed on the placement plane 213 , and the liquid to be filtered is introduced into the liquid storage chamber 212 , and the filtered liquid flows out from the liquid outlet 214 .

[0029] The liquid outlet 214 includes a guide tube 215 and a nozzle 218. The guide tube 215 is sleeved on the outside of the nozzle 218. The lower end of the nozzle 218 is inclined. The guide tube 215 extends downward beyond the lower end of the nozzle 218. The nozzle 218 is located in the middle of the guide tube 215. The lower end of the nozzle 218 is inclined to facilitate liquid outflow and prevent splashing.

[0030] A vent hole 216 is provided on the outer wall of the guide tube 215, and the reciprocating vacuum pump 30 has an air inlet and an air outlet. The air inlet end of the reciprocating vacuum pump 30 is the air inlet, and the air outlet end of the reciprocating vacuum pump 30 is the air outlet. The air inlet is connected to the vent hole 216; a vent hole 216 is provided on the outer wall of the guide tube 215 to prevent liquid from being sucked back into the reciprocating vacuum pump 30 during filtration.

[0031] An internal thread 217 is provided in the inner wall of the lower end of the guide tube 215, and the internal thread 217 is located below the nozzle 218. A connecting tube 221 is provided on the cover body 22 of the second funnel assembly 20, and an external thread 222 is provided on the upper end of the connecting tube 221 to match the internal thread 217. The funnel body 21 of the first funnel assembly 10 and the cover body 22 of the second funnel assembly 20 are detachably connected through the cooperation of the internal thread 217 and the external thread 222; the internal thread 217 is provided in the inner wall of the lower end of the guide tube 215 of the second funnel assembly 20, so that the funnel body 21 of the second funnel assembly 20 is conveniently threadedly matched with different containers, and can be detachably connected to different containers for collecting filtrate as needed, and has a wide range of applications.

[0032] The cover body 22 of the first funnel assembly 10 is provided with an opening 223 to prevent the funnel body 21 from having excessive negative pressure. The opening 223 is arranged on the upper surface of the cover body 22; the opening 223 is convenient for preventing the first air inlet pipe 40, the second air inlet pipe 50, the air outlet pipe 60 or the funnel body 21 from having excessive negative pressure inside the funnel body 21 when blockage occurs.

[0033] The usage and principle of this vacuum filtration device are as follows:

[0034] A collecting bottle for collecting filtrate is connected to the bottom of the funnel body of the second funnel assembly; when in use, the first funnel assembly, the second funnel assembly and the collecting bottle are first fixed on a fixed frame, the cell suspension is poured into the funnel body of the first funnel assembly, and the reciprocating vacuum pump is started. A negative pressure is formed on the lower side of the funnel body, and the cell suspension flows toward the collecting bottle under the action of air pressure, wherein the liquid flows into the collecting bottle through the filter cloth / filter screen, and macromolecular substances such as cell rupture products and cytoskeleton are filtered on the filter cloth / filter screen; while the reciprocating vacuum pump draws vacuum to form a negative pressure on the lower side of the funnel body, gas will be input into the funnel body of the first funnel assembly through the air outlet end of the reciprocating vacuum pump, further increasing the speed of the mixed liquid flowing toward the collecting bottle, thereby improving the filtration efficiency; in addition, the corresponding connections between the first funnel assembly, the second funnel assembly and the reciprocating vacuum pump adopt a detachable connection method, so when cleaning, it can be disassembled for cleaning, which is convenient for cleaning, disinfection and sterilization operations.

[0035] The design focus of the utility model is that the vacuum filtration device provided by the present application improves the cell filtration efficiency, is easy to operate, can be applied to high-temperature and high-pressure sterilization, and has a wide range of applications; it solves the problems of low efficiency and inconvenient cleaning in the existing filtration process; the overall structure is convenient for disassembly, assembly and sterilization, provides a closed filtration environment, and avoids the potential problems caused by liquid backflow during the filtration process, provides negative pressure through a reciprocating vacuum pump, and at the same time increases the pressure in the funnel body of the first funnel assembly, thereby improving the filtration efficiency.

[0036] The above description is merely a preferred embodiment of the present invention and does not limit the technical scope of the present invention. Therefore, any minor modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A vacuum filtration device, characterized in that: It includes a first funnel assembly, a second funnel assembly and a reciprocating vacuum pump. The first funnel assembly and the second funnel assembly both include a funnel body and a cover body, and the cover body is detachably covered on the funnel body; the funnel body of the first funnel assembly and the cover body of the second funnel assembly are detachably connected; the funnel body of the first funnel assembly and the funnel body of the second funnel assembly are both detachably connected to the air inlet end of the reciprocating vacuum pump; the air outlet end of the reciprocating vacuum pump is detachably connected to the funnel body of the first funnel assembly.

2. A vacuum filtration device according to claim 1, characterized in that: It also includes a first air inlet pipe, a second air inlet pipe and an air outlet pipe, one end of the first air inlet pipe is connected to the lower side of the funnel body of the first funnel assembly, one end of the second air inlet pipe is connected to the lower side of the funnel body of the second funnel assembly, the other end of the first air inlet pipe and the other end of the second air inlet pipe are both connected to the air inlet end of the reciprocating vacuum pump; one end of the air outlet pipe is connected to the air outlet end of the reciprocating vacuum pump, and the other end of the air outlet pipe is connected to the upper side of the funnel body of the first funnel assembly.

3. A vacuum filtration device according to claim 1, characterized in that: The funnel body includes a funnel portion located on the upper side and a liquid outlet portion located on the lower side. The funnel portion has a liquid storage cavity for accommodating the liquid to be filtered, and the lower end of the liquid storage cavity is provided with a placement plane for placing filter paper.

4. A vacuum filtration device according to claim 3, characterized in that: The liquid outlet portion includes a guide tube and a nozzle tube. The guide tube is sleeved on the outside of the nozzle tube. The lower end of the nozzle tube is inclined and extends downward beyond the lower end of the nozzle tube.

5. A vacuum filtration device according to claim 4, characterized in that: A vent is provided on the outer wall of the guide tube. The reciprocating vacuum pump has an air inlet and an air outlet. The air inlet end of the reciprocating vacuum pump is the air inlet, and the air outlet end of the reciprocating vacuum pump is the air outlet. The air inlet is connected to the vent.

6. A vacuum filtration device according to claim 4, characterized in that: An internal thread is provided in the inner wall of the lower end of the guide tube, and the internal thread is located below the nozzle tube. A connecting tube is provided on the cover body of the second funnel assembly, and an external thread matching the internal thread is provided on the upper end of the connecting tube. The funnel body of the first funnel assembly and the cover body of the second funnel assembly are detachably connected through the cooperation of the internal thread and the external thread.

7. A vacuum filtration device according to claim 1, characterized in that: The cover of the first funnel assembly is provided with an opening to prevent excessive negative pressure on the funnel body, and the opening is provided on the upper surface of the cover.

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