Auxiliary scratching tool for six-hole plate
By setting a rectangular through hole and a 15° angle fixing plate on the cover of the six-well plate, the problem of cell crushing in the prior art is solved, and clearer and more accurate experimental results and simplified operating procedures are achieved.
Patent Information
- Application Number
- CN202422120003.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-30
- Publication Date
- 2025-08-26
- Estimated Expiration
- 2034-08-30
AI Technical Summary
The scratch rods of the prior art are prone to crush cells in cell experiments, resulting in cell debris residues, affecting the clarity and accuracy of the experimental results.
A six-well plate-assisted scratch tool is designed. The plate cover is equipped with rectangular through holes and a fixed plate at an angle of 15°. The bottom of the fixed plate does not contact the cell culture medium. The plate cover is only replaced when scratches, so as to avoid the fixed plate from contacting cells and can be repeatedly disinfected.
It improves the accuracy and clarity of experimental results, reduces operation difficulty, reduces cell debris residues, simplifies the sterilization process, and facilitates reuse.
Smart Images
Figure CN223268611U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of experimental tools, in particular to a six-well plate auxiliary scratching tool. Background Art
[0002] In the prior art, a scratch bar is provided on the cover plate, and push blocks are provided on both sides of the scratch bar. Through the arrangement of the pressure block and the second slider and other structures, when it is necessary to perform a scratch experiment on the cells in the culture dish, the scratch bar is directly inserted into the special-shaped groove. At this time, the push block pushes the first slider to move downward, and the pressure block at the bottom of the first slider pushes the second slider to move. Because part of the pressure block is slidably connected to the inside of the limit groove, as the pressure block is pressed down, the second slider will tighten toward the middle, thereby completing the angle fixation of the scratch bar. There are the following technical defects: the prior art changes scratches into indentations, and changes the horizontal scraping of cells into vertical squeezing, but does not solve the problem of residual cells after crushing, because the adherent cells themselves will release adherent substances, which causes the cell fragments after squeezing to remain in the scratch line area, resulting in an unclean background for taking pictures, making it very difficult to calculate the cell migration area, and poor experimental results.
[0003] Therefore, how to design a six-well plate auxiliary scratching tool has become an urgent problem to be solved. Utility Model Content
[0004] In view of the problems existing in the prior art, the utility model provides a six-well plate auxiliary scratching tool to solve at least one of the above technical problems.
[0005] The technical solution of the utility model is: a six-well plate auxiliary scratching tool, including a plate cover, characterized in that: a plurality of array-arranged countersunk holes are provided on the top surface of the plate cover, a rectangular through hole is provided radially of the countersunk holes, and the width of the through hole is 5 mm; a plurality of guide components are provided on the bottom surface of the plate cover, and the guide components are arranged along the length direction of the through hole; any guide component includes two fixed plates arranged at an angle of ɑ, ɑ=15°, and the bottom surface of any fixed plate does not contact the cell culture medium; the scratch pen can pass through the two fixed plates.
[0006] The utility model is improved and processed based on the six-well plate cover to fit the six-well plate for scratching. A 5mm wide slit is opened in the center of each hole on the plate cover, and two fixed plates arranged at a 15° angle are installed downward, with the bottom surface of the fixed plate not contacting the cell culture medium. The design is simple, and only the plate cover is improved. It can be directly applied to the existing six-well plates in the laboratory. It is easy to use. The six-well plate cover only needs to be replaced when scratching, and it can be replaced after scratching. The lower end of the fixed plate is higher than the culture medium level under normal circumstances to avoid contact with cells, making sterilization less difficult. In theory, it can be reused. If it is accidentally contaminated, it only needs to be soaked in alcohol or disinfected with ultraviolet light. BRIEF DESCRIPTION OF THE DRAWINGS
[0007] Figure 1 It is the main view of the utility model.
[0008] Figure 2 It is a left view of the present utility model.
[0009] Figure 3 It is a bottom view of the present utility model.
[0010] In the figure: 1. Plate cover; 2. Fixed plate. DETAILED DESCRIPTION
[0011] The present invention will be further described below with reference to the accompanying drawings.
[0012] See Figure 1-3 The structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read. They are not used to limit the conditions for the implementation of this utility model, so they have no substantial technical significance. Any modification of the structure, change in the proportional relationship or adjustment of the size should still fall within the scope of the technical content disclosed by this utility model without affecting the efficacy and purpose that can be achieved by this utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description and are not used to limit the scope of the implementation of this utility model. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of this utility model without substantially changing the technical content.
[0013] Example 1: A six-well plate auxiliary scratching tool, reference Figure 1 、 Figure 2, including a plate cover 1, which is a box body with an open bottom. The top surface of the plate cover 1 is provided with 6 array-arranged countersunk holes, and rectangular through holes are provided radially of the countersunk holes. The width of the through holes is 5 mm. Six guide components are provided on the bottom surface of the plate cover 1. The guide components are arranged along the length direction of the through holes. Any guide component includes two fixed plates 2 arranged at an angle of ɑ, ɑ=15°, and the bottom surface of any fixed plate 2 does not contact the cell culture medium; the scratch pen can pass through the two fixed plates 2. The utility model is improved and processed based on the six-well plate cover, so that it can fit the six-well plate for scratching. A 5mm wide slit is opened in the center of each hole on the plate cover, and two fixed plates arranged at a 15° angle are installed downward, with the bottom surface of the fixed plate not contacting the cell culture medium. The design is simple, and only the plate cover is improved. It can be directly applied to the existing six-well plates in the laboratory. It is easy to use. Only the six-well plate cover needs to be replaced when scratching, and it can be replaced after scratching. The lower end of the fixed plate is higher than the culture medium level under normal circumstances. The scratching plate does not contact the cells, which can avoid inter-group contamination. At the same time, it reduces exposure to the outside air during scratching, avoids foreign matter in the environment from falling into the plate, and plays a role in dust and bacteria prevention. Sterilization and disinfection are not difficult, and it can be reused in theory. If it is accidentally contaminated, it only needs to be soaked in alcohol or disinfected with ultraviolet light.
[0014] Example 2: Based on Example 1, refer to Figure 3 The spacing between the upper portions of the two fixing plates 2 arranged at an angle of ɑ is greater than the spacing between the lower portions of the two fixing plates 2. In the present invention, the spacing between the upper portions of the two fixing plates arranged at a 15° angle is greater than the spacing between the lower portions of the two fixing plates arranged at a 15° angle. This can guide the scratch pen so that the scratched line does not bend or deform, and the scratch width of each hole is consistent, thereby improving experimental accuracy, reducing operational difficulty, making the result image more beautiful, and facilitating calculation.
[0015] Example 3: Based on Example 2, the bottom surfaces of the two fixing plates 2 arranged at an angle of ɑ are spaced 1 mm apart. In the present invention, the bottom surfaces of the two fixing plates arranged at a 15° angle are spaced 1 mm apart. This guides the scratch pen, ensuring that the scratched line does not bend or deform, and that the scratch width of each hole is consistent. This improves experimental accuracy, reduces operational difficulty, and makes the result images more aesthetically pleasing and facilitates calculations.
[0016] Example 4: Based on Example 2, any one of the fixing plates 2 has a length of 3.5 cm and a width of 1.5 cm. The present invention uses a fixing plate with a length of 3.5 cm, slightly smaller than the diameter of the six-well plate for easier insertion, and a width of 1.5 cm. This ensures that the lower end of the fixing plate is higher than the normal level of the culture medium, guiding the streak pen so that the scratched line does not bend or deform, and the scratch width is consistent across each well, thereby improving experimental accuracy, reducing operational difficulty, and making the result images more aesthetically pleasing and facilitating calculations.
[0017] Example 5: Based on Example 1, the rear end of the scratch pen is pen-shaped, the front end of the scratch pen is a columnar body, and a columnar head is provided on the side of the columnar body away from the rear end. This utility model adopts a method of providing a columnar head on the front end columnar body of the scratch pen, and the columnar head is fully fitted with the bottom of the board.
[0018] Example 6: Based on Example 5, the columnar head is made of silicone material, and the columnar head deforms under external force so as to fully fit with the bottom of the board. The present invention uses a columnar head made of silicone material, which can be slightly deformed under external force so as to fully fit with the bottom of the board.
[0019] To perform the scratching, hold the stylus pen firmly against the starting point of the scratch, then apply even pressure downward. The clamps on both sides prevent the scratched line from bending or deforming, and ensure a consistent scratch width across each well. This improves experimental accuracy, reduces operational difficulty, and enhances the visual quality of the resulting images, making calculations easier.
[0020] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.
Claims
1. A six-well plate auxiliary scratching tool, comprising a plate cover (1), characterized in that: The top surface of the plate cover (1) is provided with a plurality of array-arranged countersunk holes, and a rectangular through hole is provided radially of the countersunk hole, and the width of the through hole is 5 mm. The bottom surface of the plate cover (1) is provided with a plurality of guide components, and the guide components are arranged along the length direction of the through hole. Any guide component includes two fixed plates (2) arranged at an angle of ɑ, ɑ=15°, and the bottom surface of any fixed plate (2) does not contact the cell culture medium; the scratch pen can pass through the space between the two fixed plates (2).
2. The six-well plate auxiliary scratching tool according to claim 1, characterized in that: The distance between the upper parts of the two fixing plates (2) arranged at an angle ɑ is greater than the distance between the lower parts of the two fixing plates (2).
3. The six-well plate auxiliary scratching tool according to claim 2, characterized in that: The bottom surfaces of the two fixing plates (2) arranged at an angle of ɑ are spaced 1 mm apart.
4. The six-well plate auxiliary scratching tool according to claim 2, characterized in that: Any one of the fixing plates (2) has a length of 3.5 cm and a width of 1.5 cm.
5. The six-well plate auxiliary scratching tool according to claim 1, characterized in that: The rear end of the scratch pen is in the shape of a pen, the front end of the scratch pen is in the shape of a column, and a columnar head is provided on one side of the column away from the rear end.
6. The six-well plate auxiliary scratching tool according to claim 5, characterized in that: The columnar head is made of silicone material and deforms under the action of external force so as to fully fit with the bottom of the board.