EP tube temperature control mixing instrument with low-speed centrifugation in PCR (Polymerase Chain Reaction) experiment

Abstract

The utility model discloses an EP tube temperature control mixing instrument with low-speed centrifugation in a PCR (Polymerase Chain Reaction) experiment, which relates to the field of mixing instruments and comprises a supporting base, a rotating shaft is rotatably mounted at the upper end of the supporting base, rotating rods are rotatably mounted on two sides of the rotating shaft, connecting plates are mounted on the surfaces of the rotating rods through threads, and the connecting plates are connected with the rotating shafts through threads. A pipe frame set is detachably installed on one side of a connecting plate, a user places the connecting plate on one side of the pipe frame set, a connecting bolt on the surface of the connecting plate is connected with a second connecting hole in one side of the pipe frame set, so that the connecting plate is connected with the pipe frame set, and a connecting screw hole in the surface of the connecting plate is in threaded connection with a rotating rod. The pipe frame sets are fixed to the two sides of the rotating shaft, a user EP pipe is inserted into the first pipe hole and the second pipe hole which are opposite, one end of the EP pipe is inserted into the temperature control inner container, the rotating shaft can drive the EP pipe to vertically rotate, the rotating rod can drive the pipe frame sets to transversely rotate, and solutions in the EP pipe can be conveniently mixed.

Description

Technical Field

[0001] The utility model belongs to the technical field of mixers, in particular to an EP temperature-controlled mixer with low-speed centrifugation in PCR experiments. Background Art

[0002] The principle of PCR is based on the semiconservative replication mechanism of DNA. In a specific in vitro reaction system, template DNA, primers, dNTPs (single deoxynucleotides), DNA polymerase, appropriate buffer, and Mg2+ are added to the reaction system. Through repeated thermal cycling (denaturation, annealing, extension), the target DNA fragment is exponentially amplified.

[0003] The working principle of the mixer is to use a motor to drive the rotor to rotate, causing the sample to rotate, swing or oscillate, thereby generating strong centrifugal force, inertial force or shear force to mix or stir the sample evenly;

[0004] In existing PCR tests, the solution inside the EP tube needs to be mixed or centrifuged. However, existing mixers cannot process different numbers of EP tubes simultaneously according to user needs. In addition, they cannot fix EP tubes of different sizes during use, which has certain limitations. In addition, the temperature of the solution cannot be controlled during the mixing process, which affects the mixing effect of the solution.

[0005] Therefore, it is necessary to improve the existing low-speed centrifugal EP temperature-controlled mixer technology to solve the above technical problems. Utility Model Content

[0006] The purpose of the utility model is to provide an EP tube temperature-controlled mixer with low-speed centrifugation for PCR experiments, which solves the problem that in existing PCR experiments, the solution inside the EP tube needs to be mixed or centrifuged. However, during use, the existing mixer cannot process different numbers of EP tubes at the same time according to the needs of users, and cannot fix EP tubes of different sizes during use, which has certain limitations. At the same time, during the process of mixing the solution, the temperature of the solution cannot be controlled, which affects the mixing effect of the solution.

[0007] In order to solve the above technical problems, the present invention is achieved through the following technical solutions:

[0008] The utility model is an EP temperature-controlled mixer with low-speed centrifugation in a PCR experiment, comprising a support base, a rotating shaft rotatably mounted on the upper end of the support base, rotating rods rotatably mounted on both sides of the rotating shaft, a connecting plate mounted on the surface of the rotating rod via a thread, a tube rack group detachably mounted on one side of the connecting plate, a temperature-controlled liner detachably mounted inside the tube rack group, a control structure provided on one side of the support base, an interface ring provided on the upper end of the support base, and a transparent protective cover fitted with the interface ring provided on the upper end of the support base.

[0009] Preferably, the surfaces of the tube rack group and the temperature-controlled inner container are evenly provided with first tube holes and second tube holes, and the diameter of the first tube holes is greater than the diameter of the second tube holes.

[0010] Preferably, a plurality of single pipe racks are provided inside the pipe rack group, and the plurality of single pipe racks are all hexagonal. The single pipe rack is provided with six assembly plates, and the six assembly plates are hinged to each other.

[0011] Preferably, a first connecting block and a second connecting block are fixedly installed on both sides of the assembly plate, the first connecting block and the second connecting block are symmetrically distributed, and a connecting screw hole is provided on the surface of the second connecting block. Connecting bolts are movably inserted into the surfaces of the first connecting block and the connecting plate, and the connecting bolts cooperate with the connecting screw holes.

[0012] Preferably, two mounting blocks are fixedly installed on one side of the assembly block at one end of the single pipe rack, a pop-up card bead is movably installed on one side of the mounting block, and a connecting strip is fixedly installed on one side of the assembly block at the other end of the single pipe rack, and both sides of the connecting strip are provided with card holes that cooperate with the pop-up card beads.

[0013] Preferably, a mounting bar is fixedly mounted on one side of one of the assembly plates, the mounting bar is staggered with the first tube hole and the second tube hole, and a through hole is formed in the middle of the mounting bar.

[0014] Preferably, a movable arm is movably installed in the middle of the rotating rod, and the movable arm cooperates with the through hole. The movable arm can move between 0° and 45°. The connecting plate is hexagonal, and a threaded hole is opened in the middle of the connecting plate to spirally cooperate with the rotating rod.

[0015] Preferably, the control structure includes a main switch, which is arranged in the middle of one side of the support base. A speed adjustment switch and a temperature adjustment switch are provided on one side of the support base, and a centrifugal control switch box and an inner tank power connection hole are provided on the surface of the support base.

[0016] The utility model has the following beneficial effects:

[0017] 1. In the present invention, the user places the connecting plate on one side of the pipe rack assembly, connects the connecting bolts on the surface of the connecting plate with the second connecting holes on one side of the pipe rack assembly, and connects the connecting plate to the pipe rack assembly. The connecting screw holes on the surface of the connecting plate are threadedly connected to the rotating rods, so that the pipe rack assembly is fixed on both sides of the rotating shaft. The user inserts the EP tube into the first and second tube holes relative to each other, and one end of the EP tube is inserted into the temperature-controlled inner tank. The rotating shaft can drive the EP tube to rotate vertically, and the rotating rod can drive the pipe rack assembly to rotate horizontally, so as to facilitate mixing of the solution inside the EP tube.

[0018] 2. In the present invention, in the centrifugal state, the user unfolds the single tube rack so that the six assembly blocks unfold. Multiple unfolded single tube racks can also be assembled together through the first connecting block, the second connecting block and the connecting bolts to obtain an unfolded tube rack group. The user rotates the movable arm so that the movable arm rotates and tilts 45 degrees. The unfolded tube rack group is mounted on the movable arm through the mounting strip. When the rotating shaft rotates, the EP tubes on the surface of the unfolded tube rack group can be driven to rotate, which is convenient for centrifugation.

[0019] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural diagram of the mixed state of the entire device;

[0021] Figure 2 This is a schematic diagram of the structure of the overall pipe rack group of the device;

[0022] Figure 3 It is a structural schematic diagram of the single pipe rack in the device;

[0023] Figure 4 Schematic diagram of the structure of the device in the centrifugal state.

[0024] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0025] 100. Support base; 101. Main switch; 102. Speed ​​adjustment switch; 103. Centrifugal control switch; 104. Temperature adjustment switch; 105. Inner tank power connection hole; 106. Interface ring; 200. Transparent protective cover; 300. Rotating shaft; 310. Rotating rod; 311. Movable arm; 400. Pipe rack assembly; 410. Temperature-controlled inner tank; 420. Connecting plate; 421. Connecting screw hole; 430. Single pipe rack; 431. Assembly plate; 432. First connecting block; 433. Connecting bolt; 434. Second connecting block; 435. Connecting screw hole; 436. First pipe hole; 437. Second pipe hole; 438. Mounting strip; 440. Mounting block; 441. Pop-up card bead; 442. Connecting strip. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention.

[0027] See also Figures 1 to 3 As shown, an EP controlled temperature mixer with low-speed centrifugation in a PCR experiment includes a support base 100, a rotating shaft 300 is rotatably mounted on the upper end of the support base 100, and rotating rods 310 are rotatably mounted on both sides of the rotating shaft 300. A connecting plate 420 is threadedly mounted on the surface of the rotating rod 310, and a tube rack group 400 is detachably mounted on one side of the connecting plate 420. A temperature-controlled liner 410 is detachably mounted inside the tube rack group 400. A control structure is provided on one side of the support base 100, and a first tube hole 436 and a second tube hole 437 are evenly opened on the surface of the tube rack group 400 and the temperature-controlled liner 410. The diameter of the first tube hole 436 is larger than the diameter of the second tube hole 437.

[0028] In this embodiment, the user places the connecting plate 420 on one side of the tube rack group 400, and the connecting bolt 433 on the surface of the connecting plate 420 is connected to the second connecting hole on one side of the tube rack group 400, so that the connecting plate 420 is connected to the tube rack group 400, and the connecting screw holes 435421 on the surface of the connecting plate 420 are threadedly connected to the rotating rod 310, so that the tube rack group 400 is fixed on both sides of the rotating shaft 300. The user inserts the EP tube into the corresponding first tube hole 436 and second tube hole 437, and one end of the EP tube is inserted into the temperature control inner tank 410. The rotating shaft 300 can drive the EP tube to rotate vertically, and the rotating rod 310 can drive the tube rack group 400 to rotate horizontally, which is convenient for mixing the solution inside the EP tube.

[0029] Among them, an interface ring 106 is provided at the upper end of the support base 100, and a transparent protective cover 200 is sleeved on the upper end of the support base 100, which fits with the interface ring 106. Under the action of the interface ring 106, it is convenient to fix and install the transparent protective cover 200, and under the action of the transparent protective cover 200, it is convenient to protect the rotating pipe rack group 400.

[0030] See also Figure 1 As shown, an EP controlled temperature mixer with low-speed centrifugation in a PCR experiment, the control structure includes a main switch 101, the main switch 101 is set in the middle of one side of the support base 100, a speed adjustment switch 102 and a temperature adjustment switch 104 are set on one side of the support base 100, and a centrifugal control switch 103 box and an inner tank power connection hole 105 are set on the surface of the support base 100. The speed adjustment switch 102 can adjust the speed of the rotating shaft 300 and the rotating rod 310 to multiple gears, and the temperature can be adjusted under the action of the temperature adjustment switch 104.

[0031] See also Figure 2 and Figure 3 As shown, an EP controlled temperature mixer with low-speed centrifugation in a PCR experiment, a plurality of single tube racks 430 are arranged inside the tube rack group 400, and the plurality of single tube racks 430 are all hexagonal. The single tube rack 430 is provided with six assembly plates 431, and the six assembly plates 431 are hinged to each other. A first connecting block 432 and a second connecting block 434 are fixedly installed on both sides of the assembly plate 431, respectively. The first connecting block 432 and the second connecting block 434 are symmetrically distributed, and a connecting screw hole is opened on the surface of the second connecting block 434. 435421, connecting bolts 433 are movably inserted into the surfaces of the first connecting block 432 and the connecting plate 420, and the connecting bolts 433 cooperate with the connecting screw holes 435421. Two mounting blocks 440 are fixedly installed on one side of the assembly block at one end of the single tube rack 430, and a pop-up card bead 441 is movably installed on one side of the mounting block 440. A connecting strip 442 is fixedly installed on one side of the assembly block at the other end of the single tube rack 430, and both sides of the connecting strip 442 are provided with card holes that cooperate with the pop-up card bead 441;

[0032] In this embodiment, when the six assembly plates 431 are connected at the ends, they are assembled to form a single pipe rack 430. At this time, the connecting strip 442 is inserted between the two mounting blocks 440, and the pop-up card bead 441 is inserted into the inside of the card hole, so that the six assembly plates 431 can be fixedly connected, which is convenient for the assembly of the single pipe rack 430. When one side of multiple single-group racks is fitted together, the connecting bolt 433 inside the first connecting hole is inserted into the connection of the second connecting hole, so that multiple single pipe racks 430 are grouped into a pipe rack group 400, which is convenient for adjusting the pipe rack group 400 according to the needs of the user.

[0033] See also Figure 1 and Figure 4 As shown, an EP controlled temperature-mixing instrument with low-speed centrifugation for PCR experiments has a mounting bar 438 fixedly mounted on one side of an assembly plate 431. The mounting bar 438 is staggered with the first tube hole 436 and the second tube hole 437. A through-hole is provided in the middle of the mounting bar 438. A movable arm 311 is movably mounted in the middle of a rotating rod 310. The movable arm 311 cooperates with the through-hole and can move between 0° and 45°. The connecting plate 420 is hexagonal and has a threaded hole in the middle thereof that screws with the rotating rod 310.

[0034] In this embodiment, in the centrifugal state, the user unfolds the single tube rack 430, so that the six assembly blocks are unfolded. Multiple unfolded single tube racks 430 can also be assembled together through the first connecting block 432, the second connecting block 434 and the connecting bolt 433 to obtain an unfolded tube rack group 400. The user rotates the movable arm 311 so that the movable arm 311 rotates and tilts 45°. The unfolded tube rack group 400 is mounted on the movable arm 311 via the mounting bar 438. When the rotating shaft 300 rotates, it can drive the EP tubes on the surface of the unfolded tube rack group 400 to rotate, facilitating centrifugation.

[0035] The above are only preferred embodiments of the present invention and do not limit the present invention. Any modification to the technical solutions described in the aforementioned embodiments, any equivalent replacement of some of the technical features therein, and any modification, equivalent replacement, and improvement made are within the scope of protection of the present invention.

Claims

1. An EP controlled temperature mixer with low-speed centrifugation in a PCR experiment, comprising a support base (100), characterized in that: A rotating shaft (300) is rotatably mounted on the upper end of the support base (100), and rotating rods (310) are rotatably mounted on both sides of the rotating shaft (300). A connecting plate (420) is threadedly mounted on the surface of the rotating rod (310), and a pipe rack group (400) is detachably mounted on one side of the connecting plate (420). A temperature-controlled inner liner (410) is detachably mounted inside the pipe rack group (400). A control structure is provided on one side of the support base (100), and an interface ring (106) is provided on the upper end of the support base (100). A transparent protective cover (200) that fits the interface ring (106) is sleeved on the upper end of the support base (100).

2. The EP controlled temperature mixer with low-speed centrifugation in a PCR experiment according to claim 1, characterized in that: The surfaces of the tube rack group (400) and the temperature-controlled inner container (410) are evenly provided with first tube holes (436) and second tube holes (437), and the diameter of the first tube hole (436) is larger than the diameter of the second tube hole (437).

3. The EP controlled temperature mixer with low-speed centrifugation in a PCR experiment according to claim 2, characterized in that: A plurality of single pipe racks (430) are arranged inside the pipe rack group (400), and the plurality of single pipe racks (430) are all hexagonal. The single pipe rack (430) is provided with six assembly plates (431), and the six assembly plates (431) are hinged to each other.

4. The EP controlled temperature mixer with low-speed centrifugation in a PCR experiment according to claim 3, characterized in that: A first connecting block (432) and a second connecting block (434) are fixedly installed on both sides of the assembly plate (431), and the first connecting block (432) and the second connecting block (434) are symmetrically distributed. A connecting screw hole (435) (421) is provided on the surface of the second connecting block (434). Connecting bolts (433) are movably inserted through the surfaces of the first connecting block (432) and the connecting plate (420), and the connecting bolts (433) are matched with the connecting screw holes (435) (421).

5. The EP controlled temperature mixer with low-speed centrifugation in a PCR experiment according to claim 4, characterized in that: Two mounting blocks (440) are fixedly mounted on one side of an assembly block at one end of the single tube rack (430), and a pop-up card bead (441) is movably mounted on one side of the assembly block (440). A connecting strip (442) is fixedly mounted on one side of an assembly block at the other end of the single tube rack (430), and both sides of the connecting strip (442) are provided with card holes that match the pop-up card bead (441).

6. The EP controlled temperature mixer with low-speed centrifugation in a PCR experiment according to claim 5, characterized in that: A mounting bar (438) is fixedly mounted on one side of one of the assembly plates (431), the mounting bar (438) and the first tube hole (436) and the second tube hole (437) are staggered, and a through hole is opened in the middle of the mounting bar (438).

7. The EP controlled temperature mixer with low-speed centrifugation in a PCR experiment according to claim 6, characterized in that: A movable arm (311) is movably installed in the middle of the rotating rod (310), and the movable arm (311) cooperates with the through hole. The movable arm (311) can move between 0° and 45°. The connecting plate (420) is hexagonal, and a threaded hole that screws with the rotating rod (310) is opened in the middle of the connecting plate (420).

8. The EP controlled temperature mixer with low-speed centrifugation in a PCR experiment according to claim 1, characterized in that: The control structure comprises a main switch (101), the main switch (101) being provided in the middle of one side of the support base (100), a speed regulating switch (102) and a temperature regulating switch (104) being provided on one side of the support base (100), and a centrifugal control switch (103) box and an inner tank power connection hole (105) being provided on the surface of the support base (100).

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