Hematopoietic stem cell culture bottle

Abstract

The utility model discloses a hematopoietic stem cell culture bottle, which relates to the technical field of hematopoietic stem cell culture and comprises a protection mechanism, the protection mechanism comprises a protection bottle, a bottle cap is arranged at the top of the protection bottle, a buckle is arranged on one side of the bottom of the bottle cap, a sealing gasket is arranged at the bottom of the bottle cap, and the protection bottle is arranged on the protection bottle. And a sealing groove corresponding to the sealing gasket is formed in the top of the protection bottle, a mounting groove is formed in one side of the bottom of the protection bottle, a clamping block is arranged in the mounting groove, a pressing block is arranged on one side of the clamping block, and a first elastic piece is arranged on the other side of the clamping block. The anti-falling effect can be achieved to a certain extent through the protection bottle, the culture test tube can be stably clamped through the connecting rod, the second elastic piece and the clamping block, the culture test tube is prevented from shaking and moving in the protection bottle, a certain buffering effect can be provided, and the culture test tube can be prevented from falling off. The risk that the culture test tube is possibly broken when the protection bottle falls on the ground is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of hematopoietic stem cell culture, in particular to a hematopoietic stem cell culture bottle. Background Art

[0002] Hematopoietic stem cells are found in the bone marrow, blood, and certain other tissues. They have the ability to self-renew and differentiate into various blood cell types. Their primary function is to produce and maintain blood cells in the human body. They can differentiate into different types of blood cells, including red blood cells, white blood cells, and platelets. Red blood cells carry oxygen to various parts of the body, white blood cells participate in immune defense, and platelets play a key role in hemostasis.

[0003] The existing authorization announcement number is CN218932182U, which discloses a hematopoietic stem cell culture bottle, including a culture bottle, a fixing rod fixedly connected to the top of the culture bottle, and a mounting plate fixedly connected to the top of the fixing rod, a slide groove is provided on the upper surface of the mounting plate, and a connecting hole is provided between the slide groove and the inner cavity of the culture bottle, and a threaded hole is provided on one side wall of the slide groove. The beneficial effect of the utility model is that: when in use, hematopoietic stem cells can be released by piercing the sealing film with a needle, and then the threaded rod can be rotated, and the threaded rod can move the freezing slider through the cooperation with the threaded hole, so that the slider drives the other fixing hole to move to the top of the connecting hole, so that the sealing film can be replaced for protection after adding reagents and injecting and removing hematopoietic stem cells, thereby avoiding pollution from the external environment and affecting the culture of hematopoietic stem cells.

[0004] While the aforementioned technical solution allows for the replacement of the sealing membrane after reagent addition and hematopoietic stem cell injection and removal to protect the culture from external environmental contamination, the culture bottle itself is relatively fragile. If the culture bottle is impacted or accidentally dropped, it may break or become damaged, potentially harming the hematopoietic stem cells and even preventing their continued growth and development. Therefore, we provide a hematopoietic stem cell culture bottle. Utility Model Content

[0005] The purpose of the present utility model is to provide a hematopoietic stem cell culture bottle to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned objectives, the utility model provides the following technical solutions: A hematopoietic stem cell culture bottle, comprising: a protective mechanism, the protective mechanism comprising a protective bottle, a bottle cap is provided on the top of the protective bottle, a buckle is provided on one side of the bottom of the bottle cap, a sealing gasket is provided on the bottom of the bottle cap, a sealing groove corresponding to the sealing gasket is provided on the top of the protective bottle, a mounting groove is provided on one side of the bottom of the protective bottle, a card block is provided inside the mounting groove, a pressing block is provided on one side of the card block, and a spring piece 1 is provided on the other side of the card block, spring pieces 2 are provided in multiple card slots provided on the inner wall of the protective bottle, a connecting rod is provided on the end of the connecting rod away from the inner wall of the protective bottle, a clamping block is provided on the end of the connecting rod away from the spring piece 2, a sponge base is provided on the bottom side of the inner part of the protective bottle, and a pressing mechanism is provided at the bottom of the bottle cap.

[0007] As a further preferred embodiment of the present technical solution, the clamping mechanism includes a fixed tube, and the number of the fixed tubes is four. The insides of the four fixed tubes are all slidably connected with movable rods, the outsides of the four movable rods are all provided with springs, and the ends of the four movable rods away from the fixed tubes are all fixedly connected to the pressure plate.

[0008] As a further preferred embodiment of the present technical solution, one side of the top of the protective bottle is movably connected to one side of the bottom of the bottle cap by a hinge, the other side of the bottom of the bottle cap is fixedly connected with a buckle, the bottom of the bottle cap is fixedly connected with a sealing gasket, and the bottom of the sealing gasket is docked in the sealing groove.

[0009] As a further preferred embodiment of the present technical solution, the bottom of the buckle is inserted into the mounting groove opened on the top of the protective bottle and is engaged with the external protrusion of the card block, and one end of the pressing block passes through the card slot opened on one side of the protective bottle and is fixedly connected to one side of the card block.

[0010] As a further preferred embodiment of the present technical solution, one end of the spring piece 1 is fixedly connected to the other side of the locking block, and the other end of the spring piece 1 is fixedly connected to a side of the inner wall of the installation groove away from the pressing block.

[0011] As a further preferred embodiment of the present technical solution, one end of the second spring piece is fixedly connected to the side of the slot opened on the inner wall of the protective bottle away from the connecting rod, and the other end of the second spring piece is fixedly connected to the end of the connecting rod away from the clamping block.

[0012] As a further preferred embodiment of the present technical solution, the outside of the connecting rod is slidably connected to the inner wall of the slot opened inside the protective bottle, a sponge base is fixedly connected to the bottom side of the inside of the protective bottle, a handle is fixedly connected to the side of the outside of the protective bottle away from the press block, and a culture test tube is arranged inside the protective bottle.

[0013] The utility model provides a hematopoietic stem cell culture bottle, which has the following beneficial effects:

[0014] (1) The utility model can play an anti-falling effect to a certain extent through the protective bottle. The connecting rod, the second spring piece and the clamping block can not only firmly clamp the culture tube to prevent the culture tube from shaking and moving in the protective bottle, but also provide some buffering effect, reducing the risk of the culture tube being broken due to the protective bottle falling to the ground.

[0015] (2) The utility model is that after the bottle cap is covered, the pressure plate will press the culture tube tightly. When the pressure plate contacts the top of the culture tube, the squeezing force will be transmitted to the movable rod and the spring. Therefore, the movable rod will be concave into the fixed tube, and the spring will shrink and deform, thereby avoiding excessive pressure on the culture tube to cause damage to the test tube when the culture tube is pressed tightly. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic structural diagram of a hematopoietic stem cell culture bottle of the present invention.

[0017] Figure 2 This is a schematic structural diagram of the side view of a protective mechanism for a hematopoietic stem cell culture bottle of the present invention.

[0018] Figure 3 This is a schematic structural diagram of the dissected and disassembled side of a protective mechanism for a hematopoietic stem cell culture bottle of the present invention.

[0019] Figure 4 This is a partially enlarged structural schematic diagram of a protective mechanism for a hematopoietic stem cell culture bottle of the present invention.

[0020] Figure 5 This is a structural schematic diagram of the disassembled side of a hematopoietic stem cell culture bottle pressing mechanism of the present invention.

[0021] In the figure: 1, protective mechanism; 11, protective bottle; 12, bottle cap; 13, buckle; 14, sealing gasket; 15, sealing groove; 16, mounting groove; 17, clamping block; 18, pressing block; 19, spring piece 1; 110, spring piece 2; 111, connecting rod; 112, clamping block; 113, sponge base;

[0022] 2. Clamping mechanism; 21. Pressing plate; 22. Movable rod; 23. Spring; 24. Fixed tube;

[0023] 31. Handle; 32. Culture tube. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention.

[0025] The utility model provides a technical solution: Figure 1-Figure 5As shown, in this embodiment, a hematopoietic stem cell culture bottle includes: a protection mechanism 1, the protection mechanism 1 includes a protection bottle 11, a bottle cap 12 is provided on the top of the protection bottle 11, a buckle 13 is provided on one side of the bottom of the bottle cap 12, a sealing gasket 14 is provided on the bottom of the bottle cap 12, a sealing groove 15 corresponding to the sealing gasket 14 is opened on the top of the protection bottle 11, one side of the top of the protection bottle 11 is movably connected to one side of the bottom of the bottle cap 12 through a hinge, the other side of the bottom of the bottle cap 12 is fixedly connected with the buckle 13, and the bottom of the bottle cap 12 is fixedly connected. The bottom of the protective bottle 11 is fixedly connected with a sealing gasket 14, and the bottom of the sealing gasket 14 is docked in the sealing groove 15. A mounting groove 16 is provided on one side of the bottom of the protective bottle 11. A card block 17 is provided inside the mounting groove 16. A pressing block 18 is provided on one side of the card block 17. The bottom of the buckle 13 is inserted into the mounting groove 16 opened on the top of the protective bottle 11 and is clamped with the external protrusion of the card block 17. One end of the pressing block 18 passes through the card slot opened on one side of the protective bottle 11 and is fixedly connected to one side of the card block 17. A spring piece 19 is provided on the other side of the card block 17. One end of 19 is fixedly connected to the other side of the card block 17, the other end of the spring piece 19 is fixedly connected to the side of the inner wall of the mounting groove 16 away from the pressing block 18, and the multiple card slots opened on the inner wall of the protective bottle 11 are all provided with spring pieces 2 110, and the ends of the multiple spring pieces 2 110 away from the inner wall of the protective bottle 11 are all provided with connecting rods 111, and the ends of the connecting rods 111 away from the spring piece 2 110 are provided with a clamping block 112, and one end of the spring piece 2 110 is connected to the side of the card slot opened on the inner wall of the protective bottle 11 away from the connecting rod 111. The protective bottle 11 is fixedly connected, and the other end of the second spring piece 110 is fixedly connected to the end of the connecting rod 111 away from the clamping block 112. A sponge base 113 is provided on the bottom side of the interior of the protective bottle 11. The outside of the connecting rod 111 is slidably connected to the inner wall of the card slot opened inside the protective bottle 11. The sponge base 113 is fixedly connected to the bottom side of the interior of the protective bottle 11. The side of the outside of the protective bottle 11 away from the pressing block 18 is fixedly connected with a handle 31. A culture test tube 32 is provided inside the protective bottle 11, and a pressing mechanism 2 is provided at the bottom of the bottle cap 12.

[0026] In this embodiment, when the staff needs to store the hematopoietic stem cell culture bottle, they can press the button 18. When the button 18 is pressed, a pressure will be generated, so the pressure will press the block 17 and the spring 19. Therefore, the spring 19 will shrink and deform under the pressure, and the block 17 will release the limit from the clamping ring under the pressure. Therefore, the staff can open the bottle cap 12 and place the culture tube 32 containing the hematopoietic stem cells in the protective bottle 11. However, the thickness of the tube body of the culture tube 32 is inconsistent. Therefore, when the culture tube 32 with a thicker tube body is inserted into the protective bottle 11, it will contact the four clamping blocks 112 provided inside the protective bottle 11. Therefore, these four clamping blocks 112 will expand to the surroundings. During the expansion process In the process, the clamping block 112 will drive the connecting rod 111 to slide in the groove opened on the inner wall of the protective bottle 11, and then squeeze the spring piece 2 110, and the spring piece 2 110 will shrink and deform. Therefore, the cooperation between them will firmly clamp the culture tube 32 to prevent the culture tube 32 from shaking and moving in the protective bottle 11. At the same time, when the protective bottle 11 accidentally falls to the ground, the connecting rod 111, the spring piece 2 110 and the clamping block 112 can provide some buffering effect to reduce the risk of the culture tube 32 being broken. It is mentioned here that the exterior of the protective bottle 11 can be made of stainless steel, which can have an anti-fall effect to a certain extent. At the same time, the sponge base 113 can protect the bottom of the culture base.

[0027] like Figure 2-Figure 5 As shown, the clamping mechanism 2 includes four fixed tubes 24, and the interiors of the four fixed tubes 24 are all slidably connected to movable rods 22. The exteriors of the four movable rods 22 are all provided with springs 23, and the ends of the four movable rods 22 away from the fixed tubes 24 are all fixedly connected to the pressure plate 21.

[0028] In this embodiment, after the culture test tube 32 is stored in the protective bottle 11, the staff can cover the bottle cap 12, and then the pressure plate 21 provided at the bottom of the bottle cap 12 will press the culture test tube 32. When the pressure plate 21 contacts the top of the culture test tube 32, the squeezing force will be transmitted to the movable rod 22 and the spring 23, so that the movable rod 22 will be concave into the fixed tube 24, and the spring 23 will shrink and deform, thereby avoiding excessive pressure on the test tube to cause damage when the culture test tube 32 is pressed.

[0029] The utility model provides a hematopoietic stem cell culture bottle, the specific working principle of which is as follows:

[0030] When the staff needs to store the hematopoietic stem cell culture bottle, they can press the button. When it is pressed, the pressure generated will directly act on the block 17 and the spring 19. The spring 19 will shrink and deform under the influence of this pressure. At the same time, the block 17 will also release the limit from the clamping ring due to the effect of this pressure. In this way, the staff can easily open the bottle cap 12 and place the culture tube 32 containing hematopoietic stem cells in the protective bottle 11. In order to ensure the stability of the culture tube 32 in the protective bottle 11, four clamping blocks 112 are provided inside the protective bottle 11. The design of these four clamping blocks 112 is very clever. They will contact with the culture tube 32 when it is inserted into the protective bottle 11. In the process of expansion, these clamping blocks 112 will drive the connecting rod 111 to slide in the grooves provided on the inner wall of the protective bottle 11, thereby squeezing the second spring piece 110. The second spring piece 110 will also shrink and deform after being squeezed. In this way, through the interaction between the clamping blocks 112 and the second spring piece 110, the culture tube 32 is firmly clamped in the protective bottle 11, ensuring its safety. After the culture tube 32 is properly stored in the protective bottle 11, the staff can cover the bottle cap 12. At this time, the pressure plate 21 at the bottom of the bottle cap 12 will play a role. It will further press the culture tube 32 to ensure that it will not move during transportation or storage.

[0031] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A hematopoietic stem cell culture bottle, characterized in that: include: A protective mechanism (1) includes a protective bottle (11), a bottle cap (12) is provided on the top of the protective bottle (11), a buckle (13) is provided on one side of the bottom of the bottle cap (12), a sealing gasket (14) is provided on the bottom of the bottle cap (12), a sealing groove (15) corresponding to the sealing gasket (14) is provided on the top of the protective bottle (11), a mounting groove (16) is provided on one side of the bottom of the protective bottle (11), a card block (17) is provided inside the mounting groove (16), and one side of the card block (17) is provided. There is a pressing block (18), and the other side of the card block (17) is provided with a spring piece 1 (19), and multiple card slots opened on the inner wall of the protective bottle (11) are provided with spring pieces 2 (110), and multiple spring pieces 2 (110) are provided with connecting rods (111) at one end away from the inner wall of the protective bottle (11), and a clamping block (112) is provided at one end of the connecting rod (111) away from the spring piece 2 (110), and a sponge base (113) is provided on the bottom side of the inner part of the protective bottle (11), and a pressing mechanism (2) is provided at the bottom of the bottle cap (12).

2. The hematopoietic stem cell culture bottle according to claim 1, characterized in that: The clamping mechanism (2) includes four fixed tubes (24), each of the four fixed tubes (24) being slidably connected to a movable rod (22), each of the four movable rods (22) being provided with a spring (23) on its exterior, and each of the four movable rods (22) having one end away from the fixed tube (24) being fixedly connected to the pressure plate (21).

3. The hematopoietic stem cell culture bottle according to claim 1, characterized in that: One side of the top of the protective bottle (11) is movably connected to one side of the bottom of the bottle cap (12) via a hinge, the other side of the bottom of the bottle cap (12) is fixedly connected with a buckle (13), the bottom of the bottle cap (12) is fixedly connected with a sealing gasket (14), and the bottom of the sealing gasket (14) is docked in the sealing groove (15).

4. The hematopoietic stem cell culture bottle according to claim 1, characterized in that: The bottom of the buckle (13) is inserted into the mounting groove (16) opened on the top of the protective bottle (11) and is engaged with the outer protrusion of the clamping block (17); one end of the pressing block (18) passes through the clamping groove opened on one side of the protective bottle (11) and is fixedly connected to one side of the clamping block (17).

5. The hematopoietic stem cell culture bottle according to claim 1, characterized in that: One end of the spring piece (19) is fixedly connected to the other side of the clamping block (17), and the other end of the spring piece (19) is fixedly connected to the side of the inner wall of the mounting groove (16) away from the pressing block (18).

6. The hematopoietic stem cell culture bottle according to claim 1, characterized in that: One end of the second spring piece (110) is fixedly connected to a side of a slot provided on the inner wall of the protective bottle (11) away from the connecting rod (111), and the other end of the second spring piece (110) is fixedly connected to an end of the connecting rod (111) away from the clamping block (112).

7. The hematopoietic stem cell culture bottle according to claim 1, characterized in that: The outside of the connecting rod (111) is slidably connected to the inner wall of the card slot provided inside the protective bottle (11); a sponge base (113) is fixedly connected to the bottom side of the inside of the protective bottle (11); a handle (31) is fixedly connected to the side of the outside of the protective bottle (11) away from the pressing block (18); and a culture test tube (32) is provided inside the protective bottle (11).

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