Cell culture plate

Abstract

The utility model belongs to the technical field of biological medicine, and discloses a cell culture plate which comprises a culture tube assembly plate, a plurality of sample adding holes are formed in the top end face of the culture tube assembly plate, and the bottom end of the culture tube assembly plate is open; culture tubes are longitudinally arranged in the culture tube assembly plate, the top ends of the culture tubes are open and are integrally formed and fixed at the sample adding holes, and liquid outlet holes are formed in the bottoms of the culture tubes; a bottom plate is horizontally and detachably clamped to an opening in the bottom end of the culture tube assembly plate, the upper end face of the bottom plate is a supporting end face, a plurality of plugging heads are fixed to the supporting end face, and the plugging heads correspond to the culture tubes respectively and can plug the liquid outlet holes respectively. According to the cell culture plate, the liquid outlet holes are formed in the bottoms of the culture tubes, and the bottom plates with the plugging heads are arranged to plug the liquid outlet holes, so that cell culture can be normally carried out, and the culture tubes are communicated up and down and are convenient to clean.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedicine, and more particularly to a cell culture plate. Background Art

[0002] Cell culture plates are commonly used in the biomedical field. Depending on the purpose of the experiment, they can be categorized as flat-bottom or round-bottom plates, with well counts ranging from 6, 12, 24, 48, 96, 384, and 1536. Round-bottom plates are typically used in cell killing experiments to ensure close contact between target cells and effector cells. They can also be used for analysis, chemical reactions, and sample storage, as round bottoms allow for easier liquid removal compared to flat bottoms.

[0003] Cell culture plates are often used as reusable laboratory equipment, so cleaning them is crucial. Current cleaning methods typically involve filling the holes in the plates with water or buffer and then emptying the plates. This requires repeated cleaning, but cell experiments require high levels of cleanliness, making cleaning a significant workload. With technological advancements, specialized instruments for cleaning cell culture plates have emerged, but these are expensive.

[0004] Therefore, how to provide a cell culture plate with a simple structure, no need for external instruments, and easy cleaning is a problem that needs to be solved urgently by those skilled in the art. Utility Model Content

[0005] In view of this, the utility model provides a cell culture plate, which is convenient for draining cleaning liquid by providing openings at both ends of the top and bottom of the culture tube, and also convenient for draining cleaning liquid remaining at the bottom of the culture tube, thereby facilitating cleaning.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A cell culture plate, comprising:

[0008] A culture tube assembly plate, wherein the culture tube assembly plate is hollow and has a plurality of sample loading holes on its top surface, the sample loading holes being arranged in a horizontal rectangular array, and the bottom end of the culture tube assembly plate is open;

[0009] a culture tube, wherein the number of the culture tube is at least one and the culture tube is longitudinally arranged in the culture tube assembly plate, the culture tube being a tubular structure with an open top and integrally formed and fixed to the sample loading hole, and a liquid outlet is provided at the bottom of the culture tube;

[0010] The bottom plate has one end face as a supporting end face and a plurality of plugging heads fixed thereon. The bottom plate is horizontally detachably connected to the bottom opening of the culture tube assembly plate. The plurality of plugging heads are respectively distributed corresponding to the plurality of culture tubes and respectively block the plurality of liquid outlets.

[0011] The beneficial effects of the present invention are as follows: when cell culture is carried out, the supporting end face of the bottom plate is arranged toward the culture tube assembly plate, and the bottom plate is clamped to the bottom open end of the culture tube assembly plate. At this time, the multiple plugging heads on the bottom plate are plugged into the liquid outlet holes of the multiple culture tubes in a one-to-one correspondence, so that the culture tubes can be used for cell culture; before and after the cell culture process, the culture tubes need to be cleaned. During cleaning, the clamped bottom plate is first removed from the culture tube assembly plate. At this time, the plugging heads on the bottom plate also move with the bottom plate and are removed from the liquid outlet holes, so that the culture tubes are connected from top to bottom. Then, the cover plate is separated from the top of the culture tube assembly plate. At this time, cleaning liquid is poured into the sample addition hole at the top of the culture tube assembly plate to carry out cleaning; by arranging the plugging head array on the bottom plate, the plugging heads can be conveniently inserted into the liquid outlet holes at the same time, without the need for the staff to seal the bottom of the culture tubes separately, thereby improving the efficiency of the preparation work of the culture plate; and by passing the cleaning liquid into the culture tubes connected from top to bottom for cleaning, the dirt residue on the bottom and the number of cleaning times are reduced, thereby improving the cleaning efficiency of the culture tubes.

[0012] Furthermore, the bottom plate also includes a lower end surface and a liquid storage side wall, the lower end surface is provided with a liquid inlet, and the supporting end surface, the lower end surface and the liquid storage side wall together form a liquid storage cavity.

[0013] Furthermore, there is one liquid inlet, which is opened at the middle position of the end surface of the bottom plate.

[0014] The beneficial effect of adopting the above technical solution is: by opening a liquid inlet at the bottom end of the base plate, the cleaning liquid can be conveniently introduced from the liquid inlet, and there is no need to introduce the cleaning liquid into multiple sample addition holes one by one, thereby improving the efficiency of the cleaning liquid introduction, and the liquid inlet is opened in the middle position, so that the cleaning liquid is evenly dispersed after entering the interior of the base plate, and the water pressure can be evenly distributed, so that the water outlet speed of the openings of multiple sealing heads is uniform.

[0015] Furthermore, the bottom plate can be buckled onto the top of the culture tube assembly plate, and when buckled, the plugging head is located above the sample loading hole, and the end of the plugging head is provided with a one-way opening that corresponds one-to-one with the sample loading hole.

[0016] The beneficial effect of adopting the above technical solution is that the bottom plate can be flipped to the top of the culture tube assembly plate, and the end of the sealing head can be made to correspond one-to-one with the sample addition hole. Since the sealing heads are all provided with a one-way opening, and the bottom plate is hollow and provided with a liquid inlet, the cleaning liquid can be passed from the liquid inlet into the interior of the bottom plate, and then passed into the culture tube one-to-one through the opening of the sealing head, so that the bottom plate plays a role in diverting the cleaning liquid, thereby improving the efficiency of the cleaning liquid introduction.

[0017] Furthermore, the one-way opening is a conical structure, and the one-way opening direction is dispersed and expanded along the inner wall surface of the conical head along the axis to the outer peripheral wall of the blocking head.

[0018] The beneficial effects of adopting the above technical solution are: by setting a one-way opening controlled by pressure, the opening of the plugging head is opened during the cleaning process due to the outward pressure from the inside, so that the cleaning process is completed smoothly; when culturing, because the plugging head is inserted into the liquid outlet, the culture liquid in the culture tube generates pressure on the outside of the plugging head, so that the opening of the plugging head is closed, which can prevent the culture liquid in the culture tube from escaping, and ensure the normal progress of the cleaning process and the culture process.

[0019] Furthermore, the plugging head is made of polytetrafluoroethylene and the diameter of the opening of the expanded cone tip is larger than the diameter of the liquid outlet.

[0020] The beneficial effect of adopting the above technical solution is: during cleaning, the inside of the end of the plugging head will be squeezed by the cleaning liquid, causing its outer wall to disperse along the axis to the outer wall, thereby enlarging the opening of the cone tip, and ensuring that the enlarged opening is always larger than the diameter of the liquid outlet, so that the flow rate of the cleaning liquid through the cone tip opening is greater than the flow rate through the liquid outlet, thereby causing the cleaning liquid to accumulate inside the culture tube, and the liquid level of the cleaning liquid inside the culture tube rises, while the inner wall of the culture tube can be cleaned. After the cleaning liquid is stopped from being introduced, it is gradually discharged from the liquid outlet, that is, the diameter of the cone tip opening is larger than the diameter of the liquid outlet, thereby ensuring the cleaning effect of the inner wall of the culture tube; and the elastic material of polytetrafluoroethylene material is used as the material for making the plugging head, because it has excellent chemical stability and non-stickiness, it is suitable for culture environments that require high-temperature disinfection and corrosion resistance.

[0021] Furthermore, the surface is an arc surface, and the convex surface of the arc surface faces a direction away from the center of the sample addition well;

[0022] Alternatively, the bottom end surface of the culture tube is a plane, and the plane is parallel to the support end surface.

[0023] The beneficial effects of adopting the above technical solution are: the bottom end of the culture tube is set to a circular arc surface, aiming to improve the efficiency and quality of cell culture while reducing the inconvenience in culture operations; and because the bottom end of the culture tube is a circular arc surface, no cleaning liquid will remain in the bottom end of the culture tube, thereby ensuring the cleanliness and hygiene of the cell culture plate, making cleaning more convenient and thorough; the bottom end of the culture tube is set to a plane and parallel to the supporting end surface, so that the culture tube can be placed horizontally, increasing the contact area, reducing cell aggregation at the bottom, and contributing to the uniform distribution of the culture medium, thereby improving the cell culture effect.

[0024] Furthermore, the liquid outlet is opened at the center of the bottom end surface of the culture tube.

[0025] The beneficial effect of adopting the above technical solution is that: the arc surface bends in the direction away from the top opening of the culture tube. After the cleaning liquid is injected into the culture tube, the cleaning liquid can flow along the inner wall of the tube to the center of the arc surface, and then flow out from the liquid outlet at the center of the arc surface, thereby improving the discharge effect of the cleaning liquid; when the liquid outlet is located at the center of the bottom plane of the culture tube, it can ensure that the culture medium or other liquid is discharged more evenly, avoiding the cells in the local area being affected by the shear force due to the liquid flow.

[0026] Furthermore, the end of the plugging head passes through the liquid outlet and is inserted into the bottom end of the culture tube;

[0027] Alternatively, the top end of the plugging head is in sealing contact with the outer end of the liquid outlet.

[0028] The beneficial effects of adopting the above technical solution are: inserting the sealing head through the liquid outlet into the tube body or making the top end of the sealing head just clamped at the liquid outlet, both can play a sealing role on the bottom of the culture tube; when the top end of the sealing head is located inside the culture tube, it can improve the sealing performance and prevent the leakage of culture fluid. When the top end of the sealing head abuts against the liquid outlet, during cell culture, it can create a relatively flat culture environment for the cells, which is conducive to cell growth and culture.

[0029] Furthermore, it also includes a cover plate, which is detachably covered on the top of the culture tube assembly plate.

[0030] The beneficial effect of adopting the above technical solution is: by setting a cover, the cover can be placed on the top of the culture tube assembly plate during cell culture. Its benefits include improving heating performance, preventing contamination, protecting the cell culture environment, reducing water evaporation, facilitating observation and operation, improving experimental safety, and supporting a variety of culture conditions, which helps to improve the efficiency and quality of cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying any creative work.

[0032] Figure 1 This is a schematic diagram of the three-dimensional structure of the cell culture plate of the present invention.

[0033] Figure 2 for Figure 1 Schematic diagram of the top view structure.

[0034] Figure 3 for Figure 1 Schematic diagram of the upward-looking structure.

[0035] Figure 4 This is a schematic diagram of the top structure of the base plate of the present invention.

[0036] Figure 5 It is a structural schematic diagram of the cultivation state of the utility model.

[0037] Figure 6 for Figure 5 Schematic diagram of the enlarged structure of part A.

[0038] Figure 7 This is a structural diagram of the separation of the culture tube assembly plate and the base plate of the utility model.

[0039] Figure 8 It is a structural schematic diagram of the cleaning state of the utility model.

[0040] Figure 9 for Figure 8 Schematic diagram of the enlarged structure of part B.

[0041] Figure 10 This is a structural diagram of placing a cover plate in the utility model.

[0042] Figure 11 It is a structural schematic diagram of the sealing head end portion abutting against the liquid outlet hole of the present invention.

[0043] Figure 12 for Figure 11 Schematic diagram of the enlarged structure of part C.

[0044] Among them, 1-culture tube assembly plate, 11-sample addition hole, 2-culture tube, 21-liquid outlet, 3-bottom plate, 31-support end surface, 32-sealing head, 33-liquid inlet, 34-lower end surface, 35-liquid storage side wall, 4-cover plate. DETAILED DESCRIPTION

[0045] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0046] The embodiment of the present utility model discloses a cell culture plate, comprising: a culture tube assembly plate 1, the culture tube assembly plate 1 is hollow inside and has a plurality of sample addition holes 11 on its top surface, the sample addition holes 11 are distributed in a rectangular array, and the bottom end of the culture tube assembly plate 1 is open; a plurality of culture tubes 2 are longitudinally arranged in the culture tube assembly plate 1, the top ends of the culture tubes 2 are all open and integrally formed and fixed at the sample addition holes 11, and the bottom ends of the plurality of culture tubes 2 are all provided with liquid outlet holes 21; a bottom end of the open bottom end of the culture tube assembly plate 1 is horizontally and detachably clamped with a bottom plate 3, the upper end surface of the bottom plate 3 is a supporting end surface 31 and a plurality of blocking heads 32 are fixed thereon, the plurality of blocking heads 32 are respectively distributed corresponding to the plurality of culture tubes 2 and can respectively block the plurality of liquid outlet holes 21.

[0047] In one embodiment of the base plate 3 of the present invention, the base plate 3 further comprises a lower end surface 34 and a liquid storage side wall 35. The lower end surface 34 defines a liquid inlet 33. The supporting end surface 31, the lower end surface 34, and the liquid storage side wall 35 together form a liquid storage cavity. The base plate 3 can also be buckled onto the top of the culture tube assembly plate 1. When buckled, the sealing head 32 is positioned above the sample loading wells 11. The end of the sealing head 32 defines a one-way opening that corresponds one-to-one with each sample loading well 11.

[0048] The bottom plate 3 can be flipped to the top of the culture tube assembly plate 1, and the ends of the sealing heads 32 can be made to correspond one-to-one with the sample addition holes 11. Since the sealing heads 32 are all provided with one-way openings, and the bottom plate 3 is hollow inside and provided with a liquid inlet, the cleaning liquid can be passed into the bottom plate from the liquid inlet, and then passed into the culture tubes 2 one-to-one through the openings of the sealing heads 32, so that the bottom plate 3 plays a role in diverting the cleaning liquid, thereby improving the efficiency of the cleaning liquid introduction.

[0049] In the above embodiment, there is only one liquid inlet 33, which is located in the middle of the end surface of the base plate 3. By providing a single liquid inlet 33 at the bottom end of the base plate 3, the cleaning liquid can be easily introduced through the liquid inlet 33, eliminating the need to introduce the cleaning liquid into multiple sample wells one by one, thereby improving the efficiency of the cleaning liquid introduction. Furthermore, the central location of the liquid inlet 33 ensures that the cleaning liquid is evenly dispersed after entering the base plate 3, and the water pressure is evenly distributed, ensuring a uniform water outflow rate from the openings of the multiple plugging heads 32.

[0050] In the above embodiment, the one-way opening is a conical structure, and its one-way opening is arranged to expand in a dispersed manner along the inner wall of the conical head along the axis to the outer peripheral wall of the plugging head 32. By providing a pressure-controlled one-way opening, the opening of the plugging head 32 is kept open during the cleaning process due to the outward pressure from the inside, thus smoothly completing the cleaning process. When culturing, due to the plugging head 32 being inserted into the liquid outlet, the culture liquid in the culture tube 2 exerts pressure on the outside of the plugging head 32, causing the opening of the plugging head 32 to close, thus preventing the culture liquid in the culture tube 2 from escaping and ensuring the normal progress of the cleaning and culturing processes.

[0051] In the above embodiment, the plugging head 32 is made of an elastic polytetrafluoroethylene material, and the diameter of the opening at the expanded cone tip is larger than the diameter of the liquid outlet 21. During cleaning, the interior of the end of the plugging head 32 is squeezed by the cleaning liquid, causing its outer wall to expand along the axis toward the outer wall, thereby enlarging the opening at the cone tip. This enlarged opening is always larger than the diameter of the liquid outlet, so that the flow rate of the cleaning liquid through the cone tip opening is greater than the flow rate through the liquid outlet. This causes the cleaning liquid to accumulate inside the culture tube 2, raising the liquid level inside the culture tube and simultaneously cleaning the inner wall of the culture tube. After the flow of cleaning liquid stops, it gradually drains from the liquid outlet 21. That is, the diameter of the cone tip opening is larger than the diameter of the liquid outlet, ensuring the cleaning effect on the inner wall of the culture tube 2. In addition, the elastic polytetrafluoroethylene material used to make the plugging head 32 has excellent chemical stability and non-stickiness, making it suitable for environments requiring high-temperature disinfection and corrosion resistance.

[0052] In one embodiment of the culture tube 2 of the present invention, the bottom end surface of the culture tube 2 is an arc surface, and the convex surface of the arc surface faces the direction away from the center of the sample addition hole 11; or, the bottom end surface of the culture tube 2 is a plane, and the plane is parallel to the support end surface 31. Setting the bottom end of the culture tube 2 as an arc surface is intended to improve the efficiency and quality of cell culture, while reducing the inconvenience in culture operations; and because the bottom end of the culture tube 2 is an arc surface, no cleaning liquid will remain in the bottom end of the culture tube 2, thereby ensuring the cleanliness and hygiene of the cell culture plate, making cleaning more convenient and thorough; setting the bottom end of the culture tube 2 as a plane and being parallel to the support end surface 31 allows the culture tube 2 to be placed horizontally, increasing the contact area, reducing cell aggregation at the bottom, and facilitating the uniform distribution of the culture medium, thereby improving the cell culture effect.

[0053] In the above embodiment, the liquid outlet 21 is provided at the center of the bottom surface of the culture tube 2. This arcuate surface curves away from the top opening of the culture tube 2. When cleaning liquid is injected into the culture tube 2, the cleaning liquid flows along the inner wall of the tube to the center of the arcuate surface and then flows out of the liquid outlet at the center of the arcuate surface, thereby improving the cleaning liquid discharge efficiency. When the liquid outlet 21 is located at the center of the bottom plane of the culture tube 2, it can ensure that the culture medium or other liquid is discharged more evenly, preventing the cells in a local area from being affected by shear forces caused by the liquid flow.

[0054] In one embodiment of the present invention, the end of the plugging head 32 is inserted into the bottom end of the culture tube through the liquid outlet 21; or, the top end of the plugging head 32 is in sealing contact with the outer end of the liquid outlet 21. Inserting the plugging head 32 through the liquid outlet 21 into the tube body or making the top end of the plugging head 32 just snap into the liquid outlet can both seal the bottom of the culture tube 2. When the top end of the plugging head 32 is inside the culture tube 2, the sealing performance can be improved to prevent leakage of the culture fluid. When the top end of the plugging head 32 is in contact with the liquid outlet 21, a relatively flat culture environment can be created for the cells during cell culture, which is conducive to cell growth and cultivation.

[0055] Other embodiments of the present invention further include a cover plate 4 , which is detachably mounted on the top of the culture tube assembly plate 1 .

[0056] In the above embodiment, the cover plate 4 can have the same structure as the bottom plate 3 and a liquid storage cavity is provided inside the cover plate 4 and a liquid inlet is provided on its top surface, and openings distributed in a rectangular array are provided on the bottom surface. The bottom surface of the cover plate 4 can be removably covered and connected to the top of the culture tube assembly plate 1, and the multiple openings are respectively connected to the multiple sample loading holes 11, so that the culture fluid or cleaning fluid can be accurately introduced into the corresponding culture tube 2 for culture or cleaning. When cleaning, it is only necessary to remove the bottom plate 3 from the bottom of the culture tube assembly plate 1, and there is no need to flip the bottom plate 3 over and cover it on the top of the culture tube assembly plate 1, which facilitates the cleaning process. At the same time, the diameter of the bottom opening of the cover plate is larger than the diameter of the liquid outlet 21, so that the entry speed of the cleaning fluid is greater than the outflow speed, which is convenient for cleaning the wall of the culture tube 2.

[0057] In addition, other embodiments of the present invention further include a controller and a heating water tank. A temperature sensor is installed within the hollow interior of the culture tube assembly plate 1. The temperature sensor and the electric heating mechanism within the heating water tank are connected to the controller for signal communication. During incubation, the controller controls the heating water tank, located at the bottom of the culture tube assembly plate 1, to heat the air within the interior of the culture tube assembly plate 1. The temperature is then detected by the temperature sensor and transmitted to the micro-temperature sensor, thereby ensuring that the ambient temperature of the culture tubes 2 is more suitable for incubation, thereby improving the cell culture effect.

[0058] The working principle of a cell culture plate in the utility model is:

[0059] When cell culture is carried out, the supporting end face of the bottom plate is arranged toward the culture tube assembly plate, and the bottom plate is clamped to the open bottom end of the culture tube assembly plate. At this time, the multiple plugging heads on the bottom plate are plugged into the liquid outlet holes of the multiple culture tubes one by one, and the openings at the ends of the plugging heads are squeezed by the culture fluid to be closed, so that the culture tubes can be used for cell culture. At the same time, according to the type of cultured cells, the cover plate can be placed on the top of the culture tube assembly plate to isolate the culture environment, and the cover plate can also be set to a structure with an open top and multiple openings in an array at the bottom, which can facilitate the introduction of culture fluid into the culture tubes. Before and after the cell culture process, the culture tubes need to be cleaned. For cleaning, first remove the clamped bottom plate from the culture tube assembly plate. At this time, the blocking head on the bottom plate also moves with the bottom plate and moves out from the liquid outlet, so that the culture tube is connected from top to bottom. Then separate the cover plate from the top of the culture tube assembly plate, and then cover the bottom plate on the top of the culture tube assembly plate so that multiple blocking heads correspond to the sample addition holes one by one. At this time, pour the cleaning liquid from the liquid inlet to clean. By arranging the blocking head array on the bottom plate and the separately set liquid inlet, the staff only needs to pass the cleaning liquid through the liquid inlet, and there is no need to infuse the cleaning liquid into multiple sample addition holes in turn, thereby improving the cleaning efficiency of the culture tube.

[0060] Therefore, the cell culture plate of the present invention, by providing a culture tube with a liquid outlet and a bottom plate with a sealing head that can seal the culture tube, not only ensures the normal progress of the culture process, but also can complete the cleaning of the culture tube by removing the bottom plate from the bottom and connecting the cover to the top, and corresponding to the sample addition hole, and introducing the cleaning liquid through the liquid inlet on the bottom plate. The cell culture plate has a simple structure and is easy to operate, improves the cleaning efficiency of the culture tube, and can thoroughly clean the bottom of the culture tube, thereby keeping the cell culture plate clean and hygienic.

[0061] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. Reference can be made to the common and similar parts between the various embodiments. For the devices disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple, and the relevant parts can be referred to the method description.

[0062] The above description of the disclosed embodiments will enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A cell culture plate, characterized in that include: A culture tube assembly plate (1), wherein the interior of the culture tube assembly plate (1) is hollow and a plurality of sample addition holes (11) are provided on the top surface thereof, wherein the sample addition holes (11) are distributed in an array, and the bottom end of the culture tube assembly plate (1) is open; A culture tube (2), the number of the culture tube (2) being at least one and arranged longitudinally within the culture tube assembly plate (1), the culture tube (2) being a tubular structure with an open top and integrally formed and fixed to the sample loading hole (11), and a liquid outlet (21) being provided at the bottom of the culture tube (2); A bottom plate (3), the upper end surface of the bottom plate (3) is a supporting end surface (31) and a plurality of plugging heads (32) are fixed thereon, the bottom plate (3) is horizontally detachably snap-fitted to the bottom open portion of the culture tube assembly plate (1), the plurality of plugging heads (32) are respectively distributed corresponding to the plurality of culture tubes (2) and can respectively plug the plurality of liquid outlet holes (21).

2. A cell culture plate according to claim 1, characterized in that: The bottom plate (3) further comprises a lower end surface (34) and a liquid storage side wall (35); a liquid inlet (33) is provided on the lower end surface (34); and the supporting end surface (31), the lower end surface (34) and the liquid storage side wall (35) together form a liquid storage cavity.

3. A cell culture plate according to claim 2, characterized in that: There is one liquid inlet (33) which is located in the middle of the end surface of the bottom plate (3).

4. A cell culture plate according to claim 2, characterized in that: The bottom plate (3) can also be buckled onto the top end of the culture tube assembly plate (1), and when buckled, the plugging head (32) is located above the sample addition hole (11), and the end of the plugging head (32) is provided with a one-way opening and corresponds one-to-one with the sample addition hole (11).

5. A cell culture plate according to claim 4, characterized in that: The one-way opening is a conical structure, and the one-way opening direction is dispersed and expanded along the inner wall surface of the conical head along the axis to the outer peripheral wall direction of the blocking head (32).

6. A cell culture plate according to claim 5, characterized in that: The plugging head (32) is made of polytetrafluoroethylene, and the diameter of the opening of the expanded cone tip is larger than the diameter of the liquid outlet hole (21).

7. The cell culture plate according to claim 1, characterized in that: The bottom end surface of the culture tube (2) is an arc surface, and the convex surface of the arc surface faces away from the center of the sample addition hole (11); Alternatively, the bottom end surface of the culture tube (2) is a plane, and the plane is parallel to the support end surface (31).

8. A cell culture plate according to claim 7, characterized in that: The liquid outlet (21) is provided at the center of the bottom end surface of the culture tube (2).

9. The cell culture plate according to claim 1, characterized in that: The end of the plugging head (32) passes through the liquid outlet (21) and is inserted into the bottom end of the culture tube (2); Alternatively, the top end of the sealing head (32) is in sealing contact with the outer end of the liquid outlet hole (21).

10. The cell culture plate according to claim 1, characterized in that: It also includes a cover plate (4), which is detachably covered on the top of the culture tube assembly plate (1).

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