Cell culture dish
By introducing vibration and diversion structures into the cell culture dish, the problems of cell impact and uneven mixing when adding nutrient solution are solved, uniform mixing of nutrient solution and oxygen is achieved, and the efficiency of cell culture is improved.
Patent Information
- Application Number
- CN202422692121.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-05
- Publication Date
- 2025-09-26
- Estimated Expiration
- 2034-11-05
AI Technical Summary
Existing cell culture dishes easily cause shock to cells when adding nutrient solution, and are not convenient for uniform mixing of nutrient solution and oxygen, thus affecting the culture rate.
A cell culture dish was designed, which includes a vibration structure and a diversion structure. The vibration of the culture dish is driven by a vibration motor and combined with a diversion groove and a diversion slope to ensure that the nutrient solution is evenly distributed and mixed with oxygen, avoiding cell shock.
The uniform addition of nutrient solution and uniform mixing with oxygen are achieved, which reduces cell damage, shortens culture time and improves culture efficiency.
Smart Images
Figure CN223386153U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biomedicine, in particular to a cell culture dish. Background Art
[0002] Cell culture technology can transform a single cell into a simple single cell or a minimally differentiated multi-cell through mass culture. Cell growth requires a nutrient environment, and the nutrient matrix used to maintain cell growth is called a culture medium. Culture media can be divided into liquid culture media and solid culture media according to their physical state.
[0003] When culturing cells, most people pour the nutrient solution directly into the culture dish. However, this way of adding nutrient solution may have an impact on the cells, causing cell damage, and in severe cases, it may even cause cell death. Moreover, after pouring the nutrient solution, it is inconvenient to mix it evenly with the addition of oxygen, which affects the culture rate. Utility Model Content
[0004] In response to the deficiencies of the prior art, the utility model provides a cell culture dish that solves the problem that it is inconvenient to evenly add nutrient solution into the culture dish and to evenly mix the nutrient solution with oxygen when culturing cells. The utility model aims to achieve the purpose of evenly adding nutrient solution into the culture dish and evenly mixing the nutrient solution with oxygen when culturing cells.
[0005] The utility model provides the following technical solution: a cell culture dish, comprising a shell, a top cover is provided at the top end of the shell, a base is provided at the bottom end of the shell, a vibration structure for uniformly mixing nutrient solution and oxygen is provided on the base, a distributor is fixed on the top cover, a feeding pipe is fixed at the upper end of the distributor, a discharge pipe is fixed on the surface of the distributor, a guide ring plate is fixed on the inner wall of the shell, a guide ring plate is provided with a guide structure for uniformly distributing the nutrient solution, a culture body is fixed on the inner bottom surface of the shell, and a culture structure for cell culture is provided on the culture body;
[0006] The vibration structure includes a slide groove and a slider. The slide groove is provided on the upper end surface of the base. The slider is slidably connected to the inside of the slide groove. A spring is provided inside the slide groove. A limit ring is fixed to the top of the slider. A contact inclined surface is provided at the bottom end of the housing and an inner end of the limit ring. A vibration motor is fixed on the base.
[0007] The guide structure includes a guide groove and a guide slope. The guide groove is provided at the bottom end of the guide ring plate, and the guide slope is provided inside the guide groove. An upper connecting sleeve is fixed to the top of the guide ring plate, a lower nozzle is fixed to the inner top surface of the guide groove, and a guide pipe is fixed between the upper connecting sleeve and the lower nozzle.
[0008] The culture structure includes a culture pool and a concave-convex surface. The culture pool is opened in the middle of the upper end surface of the culture body, and the concave-convex surface is opened on the outside of the upper end surface of the culture body. An air guide ring tube is fixed inside the culture body, and an air outlet pipe is fixed on the air guide ring tube. A one-way air valve is fixed to the port of the air outlet pipe.
[0009] Preferably, four groups of the limiting rings are equidistantly arranged along the circumferential direction of the base, and the four groups of the limiting rings are in close contact with the housing via contact inclined surfaces.
[0010] Preferably, one end of the elastic piece is fixedly connected to the inner wall of the slide groove, and the other end is fixedly connected to the slider, and the limiting ring is slidably connected to the base through the slide groove and the slider.
[0011] Preferably, the discharge pipe is movably connected to the upper connecting sleeve, and the top cover is movably connected to the outer shell through the discharge pipe and the upper connecting sleeve.
[0012] Preferably, the feeding pipe, distributor, discharge pipe, upper connecting sleeve, flow guide pipe and lower nozzle are connected.
[0013] Preferably, the culture pool is arranged in the middle of the annular concave-convex surface, the concave-convex surface is arranged to be high on the outside and low on the inside, and the concave-convex surface is in a wavy shape.
[0014] Preferably, the air guide ring tube is arranged on the convex side of the concave-convex surface, and the air guide ring tube is connected to an external oxygen generator through a hose.
[0015] Compared with the prior art, the present invention provides a cell culture dish with the following beneficial effects:
[0016] 1. The cell culture dish pours the nutrient solution required for culture into the distributor through the feeding tube, and the distributor can evenly transport the nutrient solution to the discharge pipes at different positions, and the nutrient solution inside the discharge pipe is discharged from the lower nozzle through the upper connecting sleeve and the guide pipe, and the nutrient solution is sprayed on the guide slope, and the nutrient solution on the guide slope splashes onto the inner wall of the shell, and then flows downward along the inner wall of the shell to the concave and convex surface, and the nutrient solution on the concave and convex surface slowly flows into the culture tank, so that the nutrient solution can be evenly poured into the culture tank and avoid impact damage to the cells in the culture tank.
[0017] 2. In the cell culture dish, the nutrient solution can flow in a wave shape when flowing from the concave and convex surface to the culture pool, and when the nutrient solution flows from the convex part of the concave and convex surface to the concave part, the nutrient solution can avoid contacting the intersection of the convex and concave parts of the concave and convex surface, that is, the nutrient solution flows in a waterfall shape during this process, so that the nutrient solution will not contact the port of the outlet pipe. At the same time, the external oxygen generator can transport oxygen to the outlet pipes at different positions through the air guide ring tube and discharge it through the one-way air valve. The discharged oxygen can be evenly mixed with the nutrient solution flowing in a waterfall shape and flow into the culture pool. Vibrating and shaking the culture substrate can make it easier for oxygen to dissolve in the culture solution, shorten the cell culture time, and improve the culture efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 This is a schematic diagram of the structure of the utility model;
[0019] Figure 2 This is a partial cross-sectional view of the structure of the utility model;
[0020] Figure 3 For this utility model Figure 2 A partial enlarged schematic diagram of the structure at center A;
[0021] Figure 4 For this utility model Figure 2 A partial enlarged schematic diagram of the structure at point B in the middle;
[0022] Figure 5 For this utility model Figure 2 A partial enlarged schematic diagram of the structure at point C in the middle.
[0023] Among them: 1. Shell; 2. Top cover; 3. Base; 31. Slide groove; 32. Slider; 33. Spring piece; 34. Limiting ring; 35. Contact slope; 36. Vibration motor; 4. Distributor; 5. Feeding pipe; 6. Discharge pipe; 7. Guide ring plate; 71. Guide groove; 72. Guide slope; 73. Upper connecting sleeve; 74. Lower nozzle; 75. Guide pipe; 8. Culture medium; 81. Culture tank; 82. Concave and convex surface; 83. Air guide ring tube; 84. Air outlet pipe; 85. One-way air valve. DETAILED DESCRIPTION
[0024] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.
[0025] See also Figure 1-5The utility model provides a cell culture dish, comprising a shell 1, a top cover 2 is provided at the top of the shell 1, a base 3 is provided at the bottom of the shell 1, a vibration structure for uniformly mixing nutrient solution and oxygen is provided on the base 3, a distributor 4 is fixed on the top cover 2, a feeding pipe 5 is fixed at the upper end of the distributor 4, a discharge pipe 6 is fixed on the surface of the distributor 4, a guide ring plate 7 is fixed on the inner wall of the shell 1, and a guide structure for uniformly distributing the nutrient solution is provided on the guide ring plate 7, a culture body 8 is fixed on the inner bottom surface of the shell 1, and a culture structure for cell culture is provided on the culture body 8;
[0026] The vibration structure includes a slide 31 and a slider 32. The slide 31 is provided on the upper end surface of the base 3. The slider 32 is slidably connected to the inside of the slide 31. The slide 31 is provided with a spring 33. The top of the slider 32 is fixed with a limit ring 34. The bottom end of the housing 1 and the inner end of the limit ring 34 are provided with a contact inclined surface 35. A vibration motor 36 is fixed to the base 3.
[0027] The guide structure includes a guide groove 71 and a guide slope 72. The guide groove 71 is provided at the bottom end of the guide ring plate 7, and the guide slope 72 is provided inside the guide groove 71. An upper connecting sleeve 73 is fixed to the top of the guide ring plate 7, and a lower nozzle 74 is fixed to the inner top surface of the guide groove 71. A guide pipe 75 is fixed between the upper connecting sleeve 73 and the lower nozzle 74.
[0028] The culture structure includes a culture pool 81 and a concave-convex surface 82. The culture pool 81 is opened in the middle of the upper end surface of the culture body 8, and the concave-convex surface 82 is opened on the outer side of the upper end surface of the culture body 8. An air guide ring tube 83 is fixed inside the culture body 8, and an air outlet pipe 84 is fixed on the air guide ring tube 83. A one-way air valve 85 is fixed at the port of the air outlet pipe 84. The nutrient solution required for the culture is poured into the distributor 4 through the feeding pipe 5, and the distributor 4 can evenly transport the nutrient solution to the discharge pipes 6 at different positions so as to add nutrient solution to the inside of the culture pool 81.
[0029] Furthermore, four groups of limiting rings 34 are equidistantly arranged along the circumferential direction of the base 3. The four groups of limiting rings 34 are tightly attached to the shell 1 through the contact inclined surfaces 35, so that the shell 1 can be limited by the four groups of limiting rings 34. When the shell 1 vibrates, the shell 1 can always be in the center position.
[0030] Furthermore, one end of the spring piece 33 is fixedly connected to the inner wall of the slide groove 31, and the other end is fixedly connected to the slider 32. The limiting ring 34 is slidably connected to the base 3 through the slide groove 31 and the slider 32, so that the slider 32 can move back and forth inside the slide groove 31 by extending or contracting the spring piece 33, so that the slider 32 can drive the limiting ring 34 to move back and forth.
[0031] Furthermore, the discharge pipe 6 is movably connected to the upper connecting sleeve 73, and the top cover 2 is movably connected to the outer shell 1 through the discharge pipe 6 and the upper connecting sleeve 73. There are multiple groups of discharge pipes 6 along the circumferential direction of the distributor 4. Through the movably connected discharge pipe 6 and the upper connecting sleeve 73, the top cover 2 can be connected to the outer shell 1 and the nutrient solution can be transported to the interior of the upper connecting sleeve 73.
[0032] Furthermore, the feeding pipe 5, the distributor 4, the discharge pipe 6, the upper connecting sleeve 73, the guide pipe 75 and the lower nozzle 74 are connected to facilitate the delivery of nutrient solution to the culture tank 81 through the feeding pipe 5, the distributor 4, the discharge pipe 6, the upper connecting sleeve 73, the guide pipe 75 and the lower nozzle 74.
[0033] Furthermore, the culture pool 81 is arranged in the middle of the annular concave-convex surface 82, and the concave-convex surface 82 is inclined with the outside higher and the inside lower. The concave-convex surface 82 is wavy in shape, which facilitates the nutrient solution to be evenly mixed and dissolved with oxygen during the process of flowing downward on the concave-convex surface 82.
[0034] Furthermore, the air guide ring tube 83 is arranged on the convex side of the concave-convex surface 82, and the air guide ring tube 83 is connected to the external oxygen generator through a hose. Multiple groups of air outlet pipes 84 are arranged along the circumferential direction of the air guide ring tube 83, so that the external oxygen generator can transport oxygen to the culture tank 81 through the air guide ring tube 83, the air outlet pipe 84 and the one-way air valve 85.
[0035] During use, the nutrient solution required for culture is poured into the distributor 4 through the feeding pipe 5, and the distributor 4 can evenly transport the nutrient solution to the discharge pipe 6 at different positions, and the nutrient solution inside the discharge pipe 6 is discharged from the lower nozzle 74 through the upper sleeve 73 and the guide pipe 75, and the nutrient solution is sprayed on the guide slope 72, and the nutrient solution on the guide slope 72 splashes onto the inner wall of the outer shell 1, and then flows downward along the inner wall of the outer shell 1 to the concave-convex surface 82, and the nutrient solution on the concave-convex surface 82 slowly flows into the culture tank 81, so that the nutrient solution can be evenly poured into the culture tank 81, and impact damage to the cells in the culture tank 81 can be avoided, and the nutrient solution can flow in a wave shape in the process of flowing from the concave-convex surface 82 to the culture tank 81, and when the nutrient solution flows from the convex part to the concave part of the concave-convex surface 82 During the process on one side, the nutrient solution is prevented from contacting the intersection of the convex and concave parts of the concave-convex surface 82, that is, the nutrient solution flows in a waterfall shape during this process, so that the nutrient solution will not contact the port of the air outlet pipe 84. At the same time, the external oxygen generator can transport oxygen to the air outlet pipe 84 at different positions through the air guide ring tube 83, and discharge it through the one-way air valve 85. The discharged oxygen can be evenly mixed with the nutrient solution flowing in a waterfall shape and flow into the culture tank 81. When the nutrient solution flows into the inside of the culture tank 81, the vibration motor 36 is started to drive the culture body 8 to vibrate through the outer shell 1, and the outer shell 1 can always be limited on the base 3 through the limiting ring 34 so that it will not shift. Therefore, shaking the culture body 8 by vibration can make it easier for oxygen to dissolve in the culture solution, shorten the cell culture time, and improve the culture efficiency.
[0036] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A cell culture dish comprising a housing (1), characterized in that: The top of the shell (1) is provided with a top cover (2), the bottom of the shell (1) is provided with a base (3), the base (3) is provided with a vibration structure for uniformly mixing the nutrient solution and oxygen, the top cover (2) is fixed with a distributor (4), the upper end of the distributor (4) is fixed with a feeding pipe (5), the surface of the distributor (4) is fixed with a discharge pipe (6), the inner wall of the shell (1) is fixed with a guide ring plate (7), the guide ring plate (7) is provided with a guide structure for uniformly distributing the nutrient solution, the inner bottom surface of the shell (1) is fixed with a culture body (8), and the culture body (8) is provided with a culture structure for cell culture; The vibration structure includes a slide groove (31) and a slider (32), wherein the slide groove (31) is provided on the upper end surface of the base (3), the slider (32) is slidably connected to the inside of the slide groove (31), a spring piece (33) is provided inside the slide groove (31), a limit ring (34) is fixed to the top end of the slider (32), a contact inclined surface (35) is provided at the bottom end of the housing (1) and an inner end of the limit ring (34), and a vibration motor (36) is fixed on the base (3); The flow guide structure comprises a flow guide groove (71) and a flow guide slope (72), wherein the flow guide groove (71) is provided at the bottom end of the flow guide ring plate (7), and the flow guide slope (72) is provided inside the flow guide groove (71). An upper connecting sleeve (73) is fixed to the top end of the flow guide ring plate (7), a lower nozzle (74) is fixed to the inner top surface of the flow guide groove (71), and a flow guide pipe (75) is fixed between the upper connecting sleeve (73) and the lower nozzle (74). The culture structure includes a culture pool (81) and a concave-convex surface (82), wherein the culture pool (81) is opened in the middle of the upper end surface of the culture body (8), and the concave-convex surface (82) is opened on the outer side of the upper end surface of the culture body (8). An air guide ring tube (83) is fixed inside the culture body (8), an air outlet pipe (84) is fixed on the air guide ring tube (83), and a one-way air valve (85) is fixed to the port of the air outlet pipe (84).
2. A cell culture dish according to claim 1, characterized in that: Four groups of the limiting rings (34) are equidistantly arranged along the circumferential direction of the base (3), and the four groups of the limiting rings (34) are in close contact with the housing (1) via the contact inclined surfaces (35).
3. A cell culture dish according to claim 1, characterized in that: One end of the spring (33) is fixedly connected to the inner wall of the slide groove (31), and the other end is fixedly connected to the slider (32). The limiting ring (34) is slidably connected to the base (3) through the slide groove (31) and the slider (32).
4. A cell culture dish according to claim 1, characterized in that: The discharge pipe (6) is movably connected to the upper connecting sleeve (73), and the top cover (2) is movably connected to the outer shell (1) through the discharge pipe (6) and the upper connecting sleeve (73).
5. The cell culture dish according to claim 1, characterized in that: The feeding pipe (5), the distributor (4), the discharge pipe (6), the upper connecting sleeve (73), the flow guide pipe (75) and the lower nozzle (74) are connected.
6. The cell culture dish according to claim 1, characterized in that: The culture pool (81) is arranged in the middle of the annular concave-convex surface (82), the concave-convex surface (82) is arranged to be high on the outside and low on the inside, and the concave-convex surface (82) is in a wavy shape.
7. The cell culture dish according to claim 1, characterized in that: The air guide ring tube (83) is arranged on the convex side of the concave-convex surface (82), and the air guide ring tube (83) is connected to an external oxygen generator through a hose.