Multifunctional cell washing preparation tube

Abstract

The utility model discloses a multifunctional cell washing preparation tube which comprises a tube body, a liquid injection opening is formed in the circumferential outer wall of the tube body, a first sealing plug is fixedly installed in the liquid injection opening, and the two ends of the tube body are fixedly connected with a first connecting tube and a second connecting tube respectively. The first filter membrane and the second filter membrane are arranged in the pipe main body. The cell washing preparation tube provided by the utility model has dual purposes, obviously simplifies the processes of cell washing and cell suspension preparation, is less affected by environmental cleanliness, and reduces the risk of cell pollution to a certain extent.

Description

Technical Field

[0001] The utility model relates to the technical field of cell biology, in particular to a multifunctional cell washing preparation tube. Background Art

[0002] During the preparation of cell injections, cells usually need to be washed, separated, and purified to ensure the quality and safety of the final product. However, the following problems still exist in the preparation of existing cell injections:

[0003] Traditional cell injection preparation equipment and methods are often complex to operate and have high requirements for the cleanliness of the experimental operating environment, which in turn leads to high preparation costs. At the same time, the existing cell injection preparation process requires the use of washing tubes and preparation tubes, and the cell suspension needs to be transferred multiple times, which not only increases the risk of cell loss, but may also introduce contamination, affecting the product quality of the prepared cell injection solution.

[0004] In response to the above problems, the present utility model document proposes a multifunctional cell washing preparation tube. Utility Model Content

[0005] In response to the shortcomings of the existing technology, the utility model provides a multifunctional cell washing preparation tube that can both wash cells and prepare cell injection solution. It is a dual-purpose tube that can be used not only in a high-cleanliness environment, but also in a low-cleanliness environment, reducing the risk of cell contamination.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solution: a multifunctional cell washing preparation tube, comprising:

[0007] A tube body, wherein a liquid injection port is formed on the circumferential outer wall of the tube body, a first sealing plug is fixedly installed on the liquid injection port, and a first connecting tube and a second connecting tube are fixedly connected at both ends of the tube body, the first connecting tube is used for washing cells, and the second connecting tube is used for preparing a cell suspension;

[0008] It also includes a first filter membrane and a second filter membrane, which are both arranged in the tube body and tightly fit with the inner wall of the tube body. The first filter membrane is located in the tube body near one end of the first connecting tube and is adapted to the first connecting tube, and is used to block cells in the cell washing solution. The second filter membrane is located in the tube body near one end of the second connecting tube and is adapted to the second connecting tube, and is used to filter impurities during the preparation of the cell suspension.

[0009] As a further description of the above solution: the first connecting tube and the second connecting tube are respectively provided with tube clamps for clamping and sealing the first connecting tube and the second connecting tube.

[0010] As a further description of the above scheme: the pipe clamp includes a clamping groove opened inside it, the clamping groove cooperates with the outer wall of the corresponding first connecting tube and the second connecting tube, the inner diameter of the clamping groove gradually decreases from one end to the other end, and the clamping groove is used to complete the extrusion closure of the corresponding first connecting tube and the second connecting tube.

[0011] As a further description of the above scheme: a support frame is fixedly provided on the side of the first filter membrane and the second filter membrane away from each other, and the two support frames are respectively used to support the corresponding first filter membrane and the second filter membrane. The interior of the two support frames is a mesh structure to avoid affecting the passage of liquid; the outer walls of the two support frames are provided with positioning grooves, and the inner wall of the tube body is fixedly installed with two positioning rings, and the two positioning rings are engaged with the corresponding positioning grooves.

[0012] As a further description of the above solution: the outer walls of the first connecting tube and the second connecting tube are both fixedly sleeved with convex rings, and the two convex rings are used to complete the positioning of adjacent pipe clamps to prevent the pipe clamps (6) from sliding off.

[0013] As a further description of the above solution: a hand-holding bar is fixedly installed on one side of the pipe clamp, and the hand-holding bar is annular and located at the edge of the pipe clamp.

[0014] As a further description of the above solution: a second sealing plug is fixedly provided on one end of the first connecting tube and the second connecting tube, for completing the sealing of one end of the first connecting tube and the second connecting tube.

[0015] As a further description of the above scheme: the outer wall of the tube body is provided with two sets of scales, the starting points of the two sets of scales are opposite, and they are respectively matched with the first connecting tube and the second connecting tube. The two sets of scales are used to complete the measurement of the liquid amount in the tube body under different usage conditions.

[0016] As a further description of the above scheme: the pore size of the first filter membrane is 10 μm, which is used to retain cells, and the pore size of the second filter membrane is 30 μm, which is used to pass cells and retain impurities.

[0017] As a further description of the above solution: a docking tube is provided on the first connecting tube and / or the second connecting tube, and a control switch is provided on both the docking tubes, and the docking tubes are connected to corresponding negative pressure collection bags.

[0018] The cell washing preparation tube provided in the present application is used for washing first and then for preparation. During washing, one end of the small-pore filter membrane is kept downward, and during preparation, the large-pore filter membrane is kept downward. When using for washing cells, the user can pull the tube clamp horizontally to slide the first connecting tube into the narrower area of ​​the corresponding clamping groove to ensure that the first connecting tube is clamped and closed; then the operator can use a syringe to transfer the cell freezing solution and the configured cell washing solution into the tube body in turn. During the transfer, the syringe needle is used to pierce the first sealing plug and complete the penetration to complete the injection of the liquid into the tube body; considering the seal inside the tube body, there will be a certain resistance to injecting liquid into the tube body, so the volume of the tube body should preferably be not less than 1.2-2 of the volume of the injection liquid. .0 times; then the operator shakes the cell liquid evenly, and needs to point the first connecting tube downward. At the same time, the corresponding docking tube can be connected to the waste liquid negative pressure collection bag. After that, the operator can turn on the control switch and pull the tube clamp on the first connecting tube in the opposite direction to complete the opening of the first connecting tube. At this time, the mixed liquid in the tube body will pass through the first filter membrane into the first connecting tube under the action of negative pressure. The first filter membrane can intercept the cells contained in the mixed liquid, and the remaining washing waste liquid will be discharged into the waste liquid negative pressure collection bag through the first connecting tube. Repeat the above operation and wash 2-3 times.

[0019] Afterwards, the operator can reclose the first connecting tube and inject the corresponding reagent into the tube body according to the above operation. The corresponding cell preparation (generally 0.9% sodium chloride injection and 20% human albumin mixed with cells) can be prepared in the tube body. During washing, since the cells are trapped on the first filter membrane, the cell preparation first fully contacts the first filter membrane, and the cells are flushed from the first filter membrane into the cell preparation. After the preparation is completed, the user can turn the second connecting tube downward and invert the entire device 180 degrees. Then, according to the above operation, the corresponding cell negative pressure collection bag is connected and the corresponding tube clamp and control switch are turned on. The cell suspension in the tube body will pass through the second filter membrane into the second connecting tube and enter the corresponding negative pressure collection bag under the action of negative pressure, thereby completing the collection of the prepared cell suspension. The second filter membrane can filter out impurities contained in the cell suspension. If the second connecting tube is not provided with a connecting tube, a second sealing plug can be provided at the end of the second connecting tube. When in use, the second sealing plug is first disinfected, and then a syringe is used to pierce the second sealing plug to provide suction to remove the filtered cell suspension.

[0020] Compared with the existing technology, the present invention has the following beneficial effects: by integrating the functions of cell washing and cell suspension preparation into a single washing preparation tube, the present invention greatly simplifies the preparation process, reduces the number of operating steps and the risk of contamination; the user only needs to inject liquid into the preparation tube through the liquid injection port and then operate the tube clamp and connecting tube to complete cell washing and cell suspension preparation, which is simple and quick to operate; at the same time, by completing the corresponding preparation or washing operations in a closed tube, the high requirements for environmental cleanliness are reduced to a certain extent;

[0021] The utility model provides a multifunctional cell washing preparation tube, which is equipped with a first filter membrane and a second filter membrane in the preparation tube, and is used for blocking cells during cell washing and filtering impurities during the preparation of cell suspensions, respectively. By reasonably setting the pore size of the filter membrane, accurate filtration of cells and impurities can be achieved, thereby improving the purity and quality of the cell preparation, and realizing dual use of one tube.

[0022] The pipe clamp design of the present invention can conveniently complete the clamping and closing of the connecting pipe, avoiding the tedious steps of using additional tools or equipment to close the connecting pipe in the traditional method; at the same time, the design of the convex ring and the handle bar also increases the convenience and stability of the user's operation;

[0023] The utility model provides two sets of scales on the tube body, respectively used to measure the amount of liquid in the preparation tube under different usage conditions; this provides the user with more intuitive and accurate liquid amount information, helping to accurately control the liquid amount during the preparation process;

[0024] In the present invention, by providing a docking tube and a control switch on the outer wall of the connecting tube, it can be conveniently connected to the negative pressure collection bag; this provides users with a more flexible and convenient way of collecting and processing cells, further improving preparation efficiency and ease of operation;

[0025] The utility model significantly simplifies the process of cell washing and cell suspension preparation, reduces the difficulty of operation and the risk of contamination; the built-in double filter membrane design achieves accurate filtration of cells and impurities; the setting of the tube clamp, convex ring and hand-held bar enhances the convenience and stability of operation; the double scale design on the tube body provides intuitive liquid volume information, which is convenient for precise control; in addition, the configuration of the connecting tube and control switch makes the collection and processing of cells more flexible, further improving the preparation efficiency and convenience. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the three-dimensional structure of a multifunctional cell washing preparation tube proposed in the present invention;

[0027] Figure 2This is a schematic cross-sectional view of a multifunctional cell washing preparation tube proposed in the present invention;

[0028] Figure 3 This is a schematic diagram of the tube clamp structure of a multifunctional cell washing preparation tube proposed in the present invention;

[0029] Figure 4 This is a schematic diagram of the enlarged structure of part A of a multifunctional cell washing preparation tube proposed in the present invention;

[0030] Figure 5 This is a schematic diagram of the butt-joint pipe installation structure of a multifunctional cell washing preparation tube proposed in the present invention.

[0031] In the figure: 1. Tube body; 2. First connecting tube; 3. Second connecting tube; 4. Liquid filling port; 5. First sealing plug; 6. Tube clamp; 7. First filter membrane; 8. Second filter membrane; 9. Second sealing plug; 10. Protruding ring; 11. Clamping groove; 12. Handle bar; 13. Support frame; 14. Positioning groove; 15. Positioning ring; 16. Scale; 17. Docking tube; 18. Control switch. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0033] Example 1: Reference Figure 1-5 , a preparation tube, comprising:

[0034] A tube body 1 is cylindrical in the middle and inverted funnel-shaped at both ends. A liquid injection port 4 is defined on the outer wall of the central circumference of the tube body 1. A first sealing plug 5 is fixedly mounted within the liquid injection port 4. The first sealing plug 5 is a puncturable rubber or silicone plug that remains sealed even after being punctured by a syringe. This plug is used to keep the liquid injection port 4 sealed when not in use, preventing external contamination. To inject liquid into the tube body 1, a syringe can be used to penetrate the first sealing plug 5 after surface disinfection.

[0035] In this embodiment, a first connecting tube 2 and a second connecting tube 3 are fixedly connected to each end of the tube body 1. These two connecting tubes are used for cell washing and cell suspension preparation, respectively. During cell washing, waste liquid from cell washing can be discharged through the first connecting tube 2; during cell suspension preparation, the prepared liquid can be discharged through the second connecting tube 3. Both connecting tubes are flexible, making them easy to close with a tube clamp.

[0036] In order to achieve effective filtration of cells and impurities, in this embodiment, a first filter membrane 7 and a second filter membrane 8 are provided in the tube main body 1. Both filter membranes are tightly fitted to the inner wall of the tube main body 1 to ensure the filtering effect. The first filter membrane 7 is located in the tube main body 1 near one end of the first connecting tube 2, and cooperates with the first connecting tube 2. During the cell washing process, the pore size of the first filter membrane 7 is 10 μm, which can retain cells and prevent them from being washed out by the washing solution. The second filter membrane 8 is located in the tube main body 1 near one end of the second connecting tube 3, and cooperates with the second connecting tube 3. During the preparation of the cell suspension, the pore size of the second filter membrane 8 is 30 μm, so that cells can pass through and impurities are retained.

[0037] To support the first filter membrane 7 and the second filter membrane 8, in this embodiment, a support frame 13 is fixedly installed on the side of each filter membrane facing away from each other. These two support frames 13 are respectively used to support the corresponding filter membrane. The interior of each support frame 13 has a mesh structure to ensure that liquid can pass through the filter membrane smoothly. At the same time, the outer wall of each support frame 13 is provided with a positioning groove 14, and the inner wall of the tube body 1 is fixedly mounted with two positioning rings 15. These two positioning rings 15 respectively engage with the corresponding positioning grooves 14, thereby securing the support frame 13 and the filter membrane.

[0038] To securely hold the first connecting tube 2 and the second connecting tube 3, two tube clamps 6 are provided in this embodiment. These clamps 6 engage with the first connecting tube 2 and the second connecting tube 3, respectively. The design of the clamps 6 allows the user to conveniently open or close the connecting tubes as needed, thereby controlling the flow of liquid.

[0039] Furthermore, in this embodiment, the pipe clamp 6 has a clamping groove 11 formed therein, which mates with the outer walls of the corresponding first connecting tube 2 and second connecting tube 3. The inner diameter of the clamping groove 11 gradually decreases from one end to the other, which enables the pipe clamp 6 to achieve a better squeeze-and-seal effect when clamping the connecting tubes.

[0040] This application can be used in the field of cell biology technology, and can also be used in other fields applicable to this application.

[0041] Example 2: Reference Figure 2 、 3 5. Improvement based on Example 1: A multifunctional cell washing preparation tube, which is applied to the field of cell biology technology;

[0042] To prevent the pipe clamp 6 from sliding off during the clamping process, in this embodiment, a raised ring 10 is fixedly mounted on the outer wall of each of the first connecting tube 2 and the second connecting tube 3. These two raised rings 10 are used to limit the position of adjacent pipe clamps 6, ensuring that the pipe clamp 6 can be stably clamped on the connecting tubes.

[0043] To facilitate user operation of the pipe clamps 6, in this embodiment, a handle bar 12 is fixedly mounted on one side of each of the two pipe clamps 6. The handle bar 12 is annular and located at the edge of the pipe clamp 6, which can increase the friction between the user and the pipe clamp 6, thereby ensuring the pulling and sliding effect of the pipe clamp 6.

[0044] To maintain the sealed state at one end of the first connecting tube 2 and the second connecting tube 3, in this embodiment, a second sealing plug 9 is fixedly installed at one end of each connecting tube. The second sealing plug 9 ensures a good seal when the connecting tube is not in use, preventing external contamination. Both second sealing plugs 9 are made of the same material as the first sealing plug 5 and can also be used for puncture and liquid collection.

[0045] To facilitate user measurement of the amount of liquid within the tube body 1, in this embodiment, two sets of scales 16 are provided on the outer wall of the tube body 1. The starting points of these two sets of scales are opposite to each other and correspond to the first connecting tube 2 and the second connecting tube 3, respectively. This allows users to select the appropriate scale for measurement based on their needs in different usage scenarios.

[0046] Furthermore, to facilitate connection to the negative pressure collection bag, in this embodiment, a docking tube 17 is provided on the outer walls of both the first connecting tube 2 and the second connecting tube 3. Each of these docking tubes is equipped with a control switch 18 for controlling the on / off switching of the negative pressure. By connecting to the negative pressure collection bag, the user can conveniently collect and transfer the corresponding liquid. In this embodiment, the lower edge of the docking tube 17 is required to contact the adjacent second sealing plug 9 to prevent liquid residue.

[0047] The greatest expected value of this design lies in the use of the technical products provided by this application to reduce the air cleanliness requirements of the preparation or filtration operation environment to a certain extent while ensuring that the cell products meet the sterility requirements.

[0048] The working principle and usage process of this technical solution are as follows:

[0049] When the cell washing preparation tube is used for washing cells, the user can pull the tube clamp 6 horizontally to slide the first connecting tube 2 into the narrow area of ​​the corresponding clamping groove to ensure that the first connecting tube 2 is clamped and closed; then the operator can use a syringe to transfer the cell freezing solution and the prepared cell washing solution into the tube body 1 in sequence. During the transfer, the syringe needle is used to pierce the first sealing plug 5 and complete the penetration, thereby completing the injection of the liquid into the tube body; considering the seal inside the tube body, there will be a certain resistance to injecting the liquid into the tube body 1, so the volume of the tube body is preferably not less than 1.2 of the volume of the injection liquid. -2.0 times; then the operator shakes the cell fluid, and needs to point the first connecting tube 2 downward. At the same time, the corresponding docking tube 17 can be connected to the waste liquid negative pressure collection bag. After that, the operator can turn on the control switch 18 and pull the tube clamp 6 on the first connecting tube in reverse to complete the opening of the first connecting tube 2; at this time, the mixed liquid in the tube body will pass through the first filter membrane 7 into the first connecting tube 2 under the action of negative pressure. The first filter membrane 7 can intercept the cells contained in the mixed liquid, and the remaining washing waste liquid will be discharged into the waste liquid negative pressure collection bag through the first connecting tube 2. Repeat the above operation and wash 2-3 times.

[0050] When preparing a cell suspension: the operator can close the first connecting tube 2 again and inject the corresponding reagent into the tube body according to the above operation, and the corresponding cell preparation (generally 0.9% sodium chloride injection and 20% human serum albumin mixed with cells) can be completed in the tube body 1. During washing, since the cells are trapped on the first filter membrane 7, the cell preparation first fully contacts the first filter membrane, and the cells are flushed from the first filter membrane into the cell preparation; after completing the preparation, the user can point the second connecting tube 3 downward and invert the entire device 180°, and then connect the corresponding cell negative pressure collection bag and turn on the corresponding tube clamp 6 and control switch 18 according to the above operation. The cell suspension in the tube body 1 will pass through the second filter membrane 8 into the second connecting tube 3, and enter the corresponding negative pressure collection bag under the action of negative pressure, and the prepared cell suspension can be collected; the second filter membrane can filter out impurities contained in the cell suspension.

[0051] If the second connecting tube 3 is not provided with a docking tube 17, a second sealing plug 9 can be provided at the end of the second connecting tube 3. When in use, the second sealing plug 9 is first disinfected, and then a syringe is used to pierce the second sealing plug 9 to provide suction to remove the filtered cell suspension.

[0052] Alternatively, a second sealing plug 9 and a butt joint 17 may be provided on the first connecting tube 2 or / and the second connecting tube 3 to connect to a negative pressure collection bag, and a syringe or a negative pressure collection bag may be selected to collect the corresponding liquid according to actual conditions.

[0053] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A multifunctional cell washing preparation tube, characterized in that: include: A tube body (1), wherein a liquid injection port (4) is provided on the circumferential outer wall of the tube body (1), a first sealing plug (5) is fixedly mounted on the liquid injection port (4), and a first connecting tube (2) and a second connecting tube (3) are fixedly connected at both ends of the tube body (1), wherein the first connecting tube (2) is used for washing cells, and the second connecting tube (3) is used for preparing a cell suspension; The invention also includes a first filter membrane (7) and a second filter membrane (8), wherein the first filter membrane (7) and the second filter membrane (8) are both arranged in the tube body (1) and are closely attached to the inner wall of the tube body (1); the first filter membrane (7) is located in the tube body (1) near one end of the first connecting tube (2) and is adapted to the first connecting tube (2) for blocking cells in the cell washing solution; the second filter membrane (8) is located in the tube body (1) near one end of the second connecting tube (3) and is adapted to the second connecting tube (3) for filtering impurities during the preparation of the cell suspension.

2. The multifunctional cell washing preparation tube according to claim 1, characterized in that: The first connecting tube (2) and the second connecting tube (3) are respectively provided with a tube clamp (6) for clamping and sealing the first connecting tube (2) and the second connecting tube (3).

3. The multifunctional cell washing preparation tube according to claim 2, characterized in that: The pipe clamp (6) includes a clamping groove (11) provided therein, the clamping groove (11) being matched with the outer wall of the corresponding first connecting pipe (2) and the second connecting pipe (3), the inner diameter of the clamping groove (11) gradually decreasing from one end to the other end, and the clamping groove (11) being used to complete the extrusion sealing of the corresponding first connecting pipe (2) and the second connecting pipe (3).

4. The multifunctional cell washing preparation tube according to claim 1, characterized in that: A support frame (13) is fixedly provided on the side of the first filter membrane (7) and the second filter membrane (8) away from each other. The two support frames (13) are respectively used to support the corresponding first filter membrane (7) and the second filter membrane (8). The interior of the two support frames (13) is a mesh structure to avoid affecting the passage of liquid; the outer walls of the two support frames (13) are provided with positioning grooves (14), and the inner wall of the tube body (1) is fixedly installed with two positioning rings (15), and the two positioning rings (15) are engaged with the corresponding positioning grooves (14).

5. The multifunctional cell washing preparation tube according to claim 3, characterized in that: The outer walls of the first connecting tube (2) and the second connecting tube (3) are both fixedly sleeved with convex rings (10), and the two convex rings (10) are used to complete the positioning of adjacent pipe clamps (6) to prevent the pipe clamps (6) from sliding off.

6. The multifunctional cell washing preparation tube according to claim 5, characterized in that: A hand-holding strip (12) is fixedly mounted on one side of the pipe clamp (6); the hand-holding strip (12) is annular and is located at the edge of the pipe clamp (6).

7. The multifunctional cell washing preparation tube according to claim 1, characterized in that: One end of each of the first connecting tube (2) and the second connecting tube (3) is fixedly provided with a second sealing plug (9) for completing the sealing of one end of the first connecting tube (2) and the second connecting tube (3).

8. The multifunctional cell washing preparation tube according to claim 1, characterized in that: The outer wall of the tube body (1) is provided with two sets of scales (16), the starting points of the two sets of scales (16) are opposite to each other, and they are respectively matched with the first connecting tube (2) and the second connecting tube (3), and the two sets of scales (16) are used to complete the measurement of the amount of liquid in the tube body (1) under different usage conditions.

9. The multifunctional cell washing preparation tube according to claim 1, characterized in that: The pore size of the first filter membrane (7) is 10 μm, and the pore size of the second filter membrane (8) is 30 μm.

10. The multifunctional cell washing preparation tube according to claim 6, characterized in that: The first connecting tube (2) and / or the second connecting tube (3) are provided with a butt joint tube (17), both of the butt joint tubes (17) are provided with a control switch (18), and the butt joint tubes (17) are connected to corresponding negative pressure collection bags.

Images