Multi-channel constant-temperature reaction instrument

Abstract

The utility model relates to the field of biological detection reaction instruments. The multi-channel constant-temperature reaction instrument comprises a shell, the shell comprises an upper shell and a lower shell which are arranged up and down, and heat dissipation holes are formed in the lower shell; the upper shell is provided with at least three reaction tanks which are arranged from left to right, and the top of each reaction tank is provided with a cover body; a reaction module is inserted into the reaction tank, and a constant-temperature heating plate is mounted at the bottom of the reaction cavity; the reaction module comprises a mounting shell and a reaction unit, the reaction unit is detachably connected with the mounting shell, and the mounting shell is longitudinally inserted into the reaction tank; the reaction unit comprises a flow guide cover, a flow dividing chamber and a reaction box which are sequentially arranged from top to bottom, a flow guide hole for introducing a sample is formed in the flow guide cover, a flow guide groove directly facing the flow guide hole is formed in the flow dividing chamber, flow dividing holes are formed in the flow guide groove, and at least three reaction cavities which are independently arranged and correspond to the flow dividing holes are formed in the reaction box; a through hole for embedding the reaction cavity is formed in the bottom of the reaction tank, and the bottom of the reaction cavity is embedded into the through hole and is in contact with the constant-temperature heating plate. Through the arrangement of the plurality of reaction tanks, different reaction modules can be conveniently replaced, and the switching of multiple samples is realized.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection reaction instruments, in particular to a nucleic acid detector. Background Art

[0002] Biological detection reaction equipment, such as nucleic acid detectors, can achieve detection accuracy through temperature control.

[0003] For example, a portable nucleic acid detection device disclosed in publication number CN218372318U relates to the field of nucleic acid detection technology and includes a box body and a box cover provided on the box body. The box body is provided with a sample loading chamber and a heating module for heating the sample loading chamber. The heating module facilitates temperature control during reaction. The drawbacks are:

[0004] 1) Existing reaction boxes have a single function and few detection channels, making it impossible to achieve simultaneous operation of multiple reactions on a single sample.

[0005] 2) It is impossible to quickly replace the reaction module. Utility Model Content

[0006] In response to the problems existing in the prior art, the present invention provides a multi-channel constant temperature reactor, which has solved at least one of the above technical problems.

[0007] The technical solution of the utility model is: a multi-channel constant temperature reaction instrument, comprising a shell, characterized in that the shell comprises an upper shell and a lower shell arranged above and below, and the lower shell is provided with heat dissipation holes;

[0008] The upper shell is provided with at least three reaction tanks arranged from left to right, and a cover is installed on the top of the reaction tank;

[0009] A reaction module is inserted into the reaction tank, and a constant temperature heating plate is installed at the bottom of the reaction chamber;

[0010] The reaction module includes a mounting shell and a reaction unit, wherein the reaction unit is detachably connected to the mounting shell, and the mounting shell is longitudinally plugged into the reaction tank;

[0011] The reaction unit includes a flow guide cover, a diversion chamber, and a reaction box, which are arranged in sequence from top to bottom. The flow guide cover is provided with a drainage hole for introducing a sample. The diversion chamber is provided with a flow guide groove facing the drainage hole. The flow guide groove is provided with a diversion hole. The reaction box is provided with at least three independently arranged reaction chambers corresponding to the diversion holes.

[0012] The bottom of the reaction tank is provided with a through hole for embedding the reaction chamber, and the bottom of the reaction chamber is embedded in the through hole and contacts the constant temperature heating plate;

[0013] The reaction module further includes an end cover. The mounting shell is provided with an opening for longitudinally inserting the end cover. The end cover is partially inserted into the opening to seal the drainage hole of the guide cover.

[0014] By optimizing the reactor structure and providing multiple reaction tanks, this utility model facilitates the replacement of different reaction modules and realizes the switching of multiple samples. The optimized reaction module structure facilitates the diversion of the same sample through the diversion chamber to different reaction chambers for reaction. The constant temperature heating plate facilitates temperature control to meet different reaction temperature requirements.

[0015] Further preferably, the mounting shell is provided with at least one chamfered structure;

[0016] The reaction tank is provided with a positioning structure matching the chamfer structure.

[0017] Avoid installation misalignment.

[0018] Further preferably, the constant temperature heating plate comprises a metal sheet, a heating plate body, a heat preservation plate and a sub-control circuit board stacked from top to bottom;

[0019] The heating plate body is a base plate with a heating wire fixed thereon;

[0020] The control lead wire of the sub-control circuit board is connected to the heating wire of the heating plate body through the metal guide column passing through the insulation plate;

[0021] A groove is provided on the metal sheet, a temperature sensor is installed on the groove, and a temperature sensing surface of the temperature sensor is in close contact with the reaction box.

[0022] It is convenient to realize the temperature sensor through the temperature sensor, and adjust the reaction temperature in the reaction box through the heating plate.

[0023] If the temperature is lower than the set reaction temperature, the sub-control circuit board controls the heating wire of the heating plate to energize and heat up. If the temperature is higher than the set reaction temperature, the sub-control circuit board controls the heating plate to power off and stop heating, thereby achieving the purpose of constant temperature control.

[0024] Further preferably, the metal sheet and the heating plate body, the heating plate and the heat preservation plate body, and the heat preservation plate and the sub-control circuit board are all bonded by a heat-conducting medium.

[0025] Further preferably, the heat-conducting medium is any one of a heat-conducting graphite sheet, a heat-conducting silicone grease and a heat-conducting silicone rubber sheet.

[0026] Further preferably, the temperature sensor is connected to the signal input terminal on the sub-control circuit board through a metal conductor passing through the heating plate and the insulation plate.

[0027] Further preferably, a main control circuit board is installed in the lower shell, and the main control circuit board controls the connection with the sub-control circuit board.

[0028] Further preferably, an introduction tube for introducing reaction reagents is fixed on the diversion chamber, and the top of the introduction tube is higher than the notch of the diversion groove;

[0029] The bottom of the introduction tube is connected to the reaction chamber in a one-to-one manner;

[0030] The diversion chamber is provided with an extension portion extending into the reaction chamber, and a through hole is provided at the bottom of the extension portion for docking and conducting with the reaction chamber.

[0031] Further preferably, a sealing gasket is sandwiched between the diversion chamber and the reaction box, and the sealing gasket is provided with a sealing portion located at the periphery of the reaction chamber and separating adjacent reaction chambers.

[0032] Avoid interference with adjacent reaction chambers.

[0033] Further preferably, buckles are connected to the left and right sides of the mounting shell;

[0034] The bottoms of the left and right sides of the reaction unit are provided with notches for embedding the buckles;

[0035] The front and rear sides of the installation shell are provided with exposure openings for partially exposing the front and rear sides of the reaction unit.

[0036] It is convenient for the detachable assembly of the installation shell and the reaction unit.

[0037] Further preferably, the metal sheet can be any thermally conductive material selected from Cu, Al, Ni, Fe, Au, Ag and alloys thereof.

[0038] More preferably, the housing and the insulation board are made of plastic sheets, such as polypropylene, polyethylene, epoxy resin, and nylon.

[0039] Further preferably, the guide cover is a non-transparent plastic part;

[0040] The diversion chamber is a non-transparent plastic part;

[0041] The reaction box is a transparent plastic part.

[0042] The reaction box is made of transparent plastic, which makes it easy to guide the external light source to enter and illuminate the sample liquid in the reaction, and finally lead the light out to the colorimetric sensor for detection.

[0043] Beneficial effects:

[0044] By optimizing the reactor structure and providing multiple reaction tanks, this utility model facilitates the replacement of different reaction modules and realizes the switching of multiple samples. The optimized reaction module structure facilitates the diversion of the same sample through the diversion chamber to different reaction chambers for reaction. The constant temperature heating plate facilitates temperature control to meet different reaction temperature requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 This is a structural diagram of a specific embodiment 1 of the present utility model;

[0046] Figure 2 This is a cross-sectional view of a specific embodiment 1 of the present utility model;

[0047] Figure 3 In the specific embodiment 1 of the present utility model Figure 2 A partial enlarged view of

[0048] Figure 4 This is an exploded view of the reaction unit of specific embodiment 1 of the present utility model;

[0049] Figure 5 This is an exploded view of the reaction module of the first embodiment of the present invention;

[0050] Figure 6 This is a partial exploded view of the specific embodiment 1 of the present utility model;

[0051] Figure 7 This is a schematic structural diagram of a reaction module of a specific embodiment 1 of the present invention;

[0052] Figure 8 This is an exploded view of the reaction unit and the mounting shell of the specific embodiment 1 of the present invention.

[0053] In the figure: 1. Upper shell; 2. Lower shell; 3. Cover; 11. Reaction unit; 12. Mounting shell; 14. Sub-control circuit board; 15. Main control circuit board; 16. Metal sheet; 18. Heating plate; 19. Temperature sensor; 20. Metal guide column; 21. Insulation plate; 23. Heat dissipation hole; 25. Diversion cover; 26. Diversion chamber; 27. End cover; 28. Sealing gasket; 29. ​​Reaction box; 30. Reaction chamber, 31 is a notch, 32 is an avoidance port, and 33 is a buckle. DETAILED DESCRIPTION

[0054] See also Figures 1 to 8Specific embodiment 1, a multi-channel constant temperature reaction instrument, comprising a shell, the shell comprising an upper shell 1 and a lower shell 2 arranged above and below, the lower shell 2 is provided with a heat dissipation hole 23; the upper shell 1 is provided with at least three reaction tanks arranged from left to right, the top of the reaction tank is provided with a cover 3; a reaction module is inserted into the reaction tank, and a constant temperature heating plate is installed at the bottom of the reaction chamber 30; the reaction module comprises a mounting shell 12 and a reaction unit 11, the reaction unit 11 is detachably connected to the mounting shell 12, and the mounting shell 12 is connected to the reaction tank Longitudinal plug-in; the reaction unit 11 includes a flow guide cover 25, a diversion chamber 26 and a reaction box 29 arranged in sequence from top to bottom. The flow guide cover 25 is provided with a drainage hole for introducing samples, the diversion chamber 26 is provided with a flow guide groove facing the drainage hole, the flow guide groove is provided with a diversion hole, and the reaction box 29 is provided with at least three reaction chambers 30 independently arranged and corresponding to the diversion holes; the bottom of the reaction groove is provided with a through hole for embedding the reaction chamber 30, and the bottom of the reaction chamber 30 is embedded in the through hole and contacts the constant temperature heating plate. The reaction module also includes an end cap 27, and the mounting shell 12 is provided with an opening for longitudinally inserting the end cap 27, and the end cap 27 is partially inserted into the opening to seal the drainage hole of the flow guide cover. The end cap is used to seal the drainage hole of the flow guide cover 25 and can seal the flow guide system. The utility model optimizes the structure of the reactor and facilitates the replacement of different reaction modules and realizes the switching of multiple samples through the provision of multiple reaction tanks. The optimized reaction module structure facilitates the diversion of the same sample through the diversion chamber 26 to different reaction chambers 30 for reaction. A constant temperature heating plate facilitates temperature control to meet varying reaction temperature requirements. The mounting housing 12 is equipped with at least one chamfered corner, and the reaction tank is equipped with a positioning structure that matches the chamfered corner, preventing misalignment during installation.

[0055] The opposite side of the deflector cover is provided with a concave structure to facilitate finger gripping.

[0056] See also Figure 8 , the left and right sides of the mounting shell 12 are connected with snaps 33; the bottoms of the left and right sides of the reaction unit are provided with notches 31 for embedding the snaps; the front and rear sides of the mounting shell 12 are provided with exposed openings for partially exposing the front and rear sides of the reaction unit. This facilitates the detachable assembly of the mounting shell and the reaction unit. The left and right sides of the exposed opening 32 are longitudinally slidably connected to the reaction unit. The upper end surface of the exposed opening 32 is abutted against the reaction unit. The bottom of the diversion chamber is connected to a cover body that is arranged around the periphery of the reaction chamber. The exposed opening 32 and the cover body abut against each other. A notch 31 is provided on the cover body.

[0057] The constant temperature heating plate comprises a metal sheet 16, a heating plate body 18, an insulation plate 21, and a sub-control circuit board 14, stacked from top to bottom. The heating plate body 18 is a base plate with a heating wire fixed thereto. The control lead wires of the sub-control circuit board 14 are connected to the heating wire of the heating plate body 18 via metal guide pins 20 that pass through the insulation plate 21. The metal sheet 16 has a groove in which a temperature sensor 19 is mounted. The temperature-sensing surface of the temperature sensor 19 is in close contact with the reaction box 29. The temperature sensor 19 facilitates temperature adjustment of the reaction temperature within the reaction box 29 through the heating plate body 18. If the temperature is lower than the set reaction temperature, the sub-control circuit board 14 controls the heating wire of the heating plate body 18 to energize and increase the temperature. If the temperature is higher than the set reaction temperature, the sub-control circuit board 14 controls the heating plate body 18 to de-energize and stop heating, thereby achieving the purpose of constant temperature control. The metal sheet 16 and the heating plate 18, the heating plate 18 and the insulation plate 21, and the insulation plate 21 and the sub-control circuit board 14 are all bonded together using a thermally conductive medium. This medium can be any of thermally conductive graphite sheets, thermally conductive silicone grease, or thermally conductive silicone sheets. The temperature sensor 19 is connected to the signal input terminal on the sub-control circuit board 14 via a metal conductor that passes through the heating plate 18 and the insulation plate 21. A main control circuit board 15 is mounted within the lower housing and controls the connection to the sub-control circuit board 14.

[0058] A feed tube for introducing reaction reagents is fixed to the diverter chamber 26. The top of the feed tube is higher than the notch of the diverter trough; the bottom of the feed tube is connected to the reaction chamber 30. The diverter chamber 26 is provided with an extension that extends into the reaction chamber 30. The bottom of the extension has a through hole that connects to the reaction chamber 30. A sealing gasket 28 is sandwiched between the diverter chamber 26 and the reaction box 29. The sealing gasket 28 is provided with a sealing portion located outside the reaction chamber 30 and separating adjacent reaction chambers to prevent interference between adjacent reaction chambers 30.

[0059] A diversion nozzle, wide at the top and narrow at the bottom, is located in the center of the diversion cover and runs through it from top to bottom. A plug is detachably connected to the diversion cover to block the diversion nozzle.

[0060] The metal sheet 16 may be any thermally conductive material selected from Cu, Al, Ni, Fe, Au, Ag, and alloys thereof.

[0061] The shell and the insulation board 21 are made of plastic sheets. The base plate is also made of plastic sheets. Materials such as polypropylene, polyethylene, epoxy resin and nylon can be selected for use.

[0062] The flow guide cover 25 is made of opaque plastic, the diversion chamber 26 is made of opaque plastic, and the reaction box 29 is made of transparent plastic. The transparent plastic structure of the reaction box 29 facilitates the introduction of external light to illuminate the reacting sample solution, ultimately directing the light to the colorimetric sensor for detection.

[0063] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A multi-channel constant temperature reactor, comprising a housing, characterized in that: The housing comprises an upper housing and a lower housing arranged above and below, and the lower housing is provided with heat dissipation holes; The upper shell is provided with at least three reaction tanks arranged from left to right, and a cover is installed on the top of the reaction tank; A reaction module is inserted into the reaction tank, and a constant temperature heating plate is installed at the bottom of the reaction chamber; The reaction module includes a mounting shell and a reaction unit, wherein the reaction unit is detachably connected to the mounting shell, and the mounting shell is longitudinally plugged into the reaction tank; The reaction unit includes a flow guide cover, a diversion chamber, and a reaction box, which are arranged in sequence from top to bottom. The flow guide cover is provided with a drainage hole for introducing a sample. The diversion chamber is provided with a flow guide groove facing the drainage hole. The flow guide groove is provided with a diversion hole. The reaction box is provided with at least three independently arranged reaction chambers corresponding to the diversion holes. The bottom of the reaction tank is provided with a through hole for embedding the reaction chamber, and the bottom of the reaction chamber is embedded in the through hole and contacts the constant temperature heating plate; The reaction module further includes an end cover. The mounting shell is provided with an opening for longitudinally inserting the end cover. The end cover is partially inserted into the opening to seal the drainage hole of the guide cover.

2. The multi-channel constant temperature reactor according to claim 1, characterized in that: The mounting shell is provided with at least one chamfered structure; The reaction tank is provided with a positioning structure matching the chamfer structure.

3. The multi-channel constant temperature reactor according to claim 1, characterized in that: The constant temperature heating plate comprises a metal sheet, a heating plate body, a heat preservation plate and a sub-control circuit board stacked from top to bottom; The heating plate body is a base plate with a heating wire fixed thereon; The control lead wire of the sub-control circuit board is connected to the heating wire of the heating plate body through the metal guide column passing through the insulation plate; A groove is provided on the metal sheet, a temperature sensor is installed on the groove, and a temperature sensing surface of the temperature sensor is in close contact with the reaction box.

4. The multi-channel constant temperature reactor according to claim 3, characterized in that: The metal sheet and the heating plate body, the heating plate body and the heat preservation plate, and the heat preservation plate and the sub-control circuit board are all bonded together by a heat-conducting medium.

5. The multi-channel constant temperature reactor according to claim 4, characterized in that: The heat-conducting medium is any one of a heat-conducting graphite sheet, a heat-conducting silicone grease and a heat-conducting silicone sheet.

6. The multi-channel constant temperature reactor according to claim 3, characterized in that: The temperature sensor is connected to the signal input terminal on the sub-control circuit board through a metal conductor passing through the heating plate and the heat preservation plate.

7. The multi-channel constant temperature reactor according to claim 1, characterized in that: Buckles are connected to the left and right sides of the mounting shell; Notches for embedding the buckles are provided at the bottoms of the left and right sides of the reaction unit; The front and rear sides of the installation shell are provided with exposure openings for partially exposing the front and rear sides of the reaction unit.

8. The multi-channel constant temperature reactor according to claim 1, characterized in that: An introduction tube for introducing reaction reagents is fixed on the diversion chamber, and the top of the introduction tube is higher than the notch of the diversion groove; The bottom of the introduction tube is connected to the reaction chamber in a one-to-one manner; The diversion chamber is provided with an extension portion extending into the reaction chamber, and a through hole is provided at the bottom of the extension portion for docking and conducting with the reaction chamber.

9. The multi-channel constant temperature reactor according to claim 1, characterized in that: A sealing gasket is sandwiched between the diversion chamber and the reaction box. The sealing gasket is provided with a sealing portion located at the periphery of the reaction chamber and separating adjacent reaction chambers.

10. The multi-channel constant temperature reactor according to claim 1, characterized in that: The deflector cover is a non-transparent plastic part; The diversion chamber is a non-transparent plastic part; The reaction box is a transparent plastic part.

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