Production system for preparing and purifying glycine

Abstract

The utility model relates to the technical field of glycine production, in particular to a production system for preparing and purifying glycine. Comprising a seed tank, a fermentation tank, a centrifugal machine, a thallus storage tank, a buffer solution storage tank, a reaction kettle, a first filtering device, a second filtering device, a first resin column, a pH adjusting kettle, a second resin column, a concentration kettle, a first crystallization kettle, a first washing kettle, a decoloring kettle, a second crystallization kettle, a second washing kettle, a drying device and a glycine storage tank. According to the production system for preparation and purification, continuous production can be achieved, and the yield, the yield and the purity of glycine are greatly improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to glycine production technical field especially relates to a glycine preparation and purification's production system. BACKGROUND

[0002] With the rapid development of biotechnology, the preparation of organic acids or amino acids by biological conversion method is attracting more and more attention. The biological conversion method mainly uses microbial free cells or purified enzymes as catalytic carriers to convert the substrate glycine into glycine. At present, foreign countries have applied for more patents on the preparation of glycine by biological conversion method, but there is no related research report on the production of glycine by biological conversion method in China. In foreign countries, the patent US5238827 of NITTO Chemical Company in Japan discloses a method for converting glycine into glycine by using bacteria strains of red ball, arthrobacter, lactobacillus, enterobacter and other bacteria as catalytic enzymes. The patent W00148234 of ASAHI Chemical Company discloses a method for preparing glycine by hydrolyzing glycine using bacteria and yeast of acinetobacter, corynebacterium, alkali bacillus, mycobacterium, rhodopseudomonas and candida, and a method for recovering and purifying glycine, such as EP1243657, US20030040085, CN1433479, N1840522. These lay a foundation for further research on the production of glycine by biological conversion. However, the yield and purity of glycine are relatively low. Therefore, it is necessary to develop a production system for the preparation and purification of glycine to solve the above problems. UTILITY MODEL CONTENT

[0003] The technical problem to be solved by the utility model is that in view of the deficiency of the prior art, a production system for the preparation and purification of glycine is provided, which can improve the yield, yield and purity of glycine by using the purification system.

[0004] To solve the above technical problems, the technical scheme of the utility model is:

[0005] The application discloses a production system for preparing and purifying glycine, which comprises a seed tank, an outlet of the seed tank being connected with a fermentation tank, an outlet of the fermentation tank being connected with a centrifuge, a discharge port of the centrifuge being connected with a bacterial body storage tank, a liquid inlet of the bacterial body storage tank being connected with a buffer solution storage tank, a discharge port of the bacterial body storage tank being connected with a reaction kettle, an outlet of the reaction kettle being connected with a first filtering device, an outlet of the first filtering device being connected with a second filtering device, an outlet of the second filtering device being connected with a first resin column, an outflow outlet of the first resin column being connected with a pH adjusting kettle, a discharge port of the pH adjusting kettle being connected with a second resin column, a liquid inlet of the second resin column being connected with an eluent pipeline, an eluent outlet of the second resin column being connected with a concentration kettle, an outflow port of the concentration kettle being connected with a first crystallization kettle, a crystal outlet of the first crystallization kettle being connected with a first washing kettle, a discharge port of the first washing kettle being connected with a decolorization kettle, a decolorization liquid outlet of the decolorization kettle being connected with a second crystallization kettle, a crystal outlet of the second crystallization kettle being connected with a second washing kettle, a discharge port of the second washing kettle being connected with a drying device, and an outlet of the drying device being connected with a glycine storage tank.

[0006] As an improved technical scheme, the first filtering device is a ceramic membrane filtering device with a pore size of 20-50 nm.

[0007] As an improved technical scheme, the second filtering device is an ultrafiltration membrane filtering device with a molecular weight cut-off of 5000-10000 Da.

[0008] As an improved technical scheme, the first resin column is a cation resin column, and the second resin column is an anion resin column.

[0009] As an improved technical scheme, the first crystallization kettle and the second crystallization kettle each comprise a kettle body, a feed inlet and a discharge port being arranged at the top and the bottom of the kettle body respectively, a crystallization mother liquor outlet being arranged at one side of the lower part of the kettle body, and a filter screen being arranged on the inner wall of the kettle body and corresponding to the crystallization mother liquor outlet; a jacket is arranged outside the kettle body, a stirring shaft is arranged inside the kettle body, one end of the stirring shaft is connected with a motor, and a plurality of stirring plates are arranged on the stirring shaft.

[0010] As an improved technical scheme, the first washing kettle and the second washing kettle each comprise a kettle body, a feed inlet and a washing liquid inlet being arranged at the top of the kettle body, a discharge port being arranged at the bottom of the kettle body, a washing liquid outlet being arranged at one side of the lower part of the kettle body, and a filter screen being arranged on the inner wall of the kettle body and corresponding to the washing liquid outlet; a rotating shaft is arranged inside the kettle body, one end of the rotating shaft is connected with a motor, a stirring frame is arranged on the rotating shaft, and a plurality of stirring rods are arranged on the stirring frame.

[0011] As an improved technical scheme, the decoloring kettle comprises a kettle body, a feeding port, a decoloring agent inlet and a solvent inlet are arranged at the top of the kettle body, a decoloring agent outlet is arranged at the bottom of the kettle body, a decoloring liquid outlet is arranged at one side of the lower part of the kettle body, a filter screen is arranged at the position corresponding to the decoloring liquid outlet on the inner wall of the kettle body, a jacket is arranged outside the kettle body, a stirring shaft is arranged inside the kettle body, one end of the stirring shaft is connected with a motor, and a plurality of stirring rods are arranged on the stirring shaft.

[0012] After the above technical scheme is adopted, the beneficial effects of the present application are as follows:

[0013] The production system for preparation and purification of glycine comprises a seed tank, the outlet of the seed tank is connected with a fermentation tank, the outlet of the fermentation tank is connected with a centrifuge, the discharge port of the centrifuge is connected with a bacterial body storage tank, the liquid inlet of the bacterial body storage tank is connected with a buffer solution storage tank, the discharge port of the bacterial body storage tank is connected with a reaction kettle, the outlet of the reaction kettle is connected with a first filtering device, the outlet of the first filtering device is connected with a second filtering device, the outlet of the second filtering device is connected with a first resin column, the effluent outlet of the first resin column is connected with a pH adjusting kettle, the discharge port of the pH adjusting kettle is connected with a second resin column, the liquid inlet of the second resin column is connected with an eluent pipeline, the eluent outlet of the second resin column is connected with a concentration kettle, the liquid outlet of the concentration kettle is connected with a first crystallization kettle, the crystal outlet of the first crystallization kettle is connected with a first washing kettle, the discharge port of the first washing kettle is connected with a decoloring kettle, the decoloring liquid outlet of the decoloring kettle is connected with a second crystallization kettle, the crystal outlet of the second crystallization kettle is connected with a second washing kettle, the discharge port of the second washing kettle is connected with a drying device, and the outlet of the drying device is connected with a glycine storage tank. In actual production, the culture medium, substrate and other components are put into the seed tank, the culture conditions are controlled, the bacterial body after cultivation is transferred into the fermentation tank filled with fermentation medium, the fermentation conditions are controlled, the fermentation liquid after fermentation is separated by the centrifuge, the collected bacterial body is transferred into the bacterial body storage tank, at the same time, the buffer solution in the buffer solution storage tank is introduced into the bacterial body storage tank, the bacterial body resuspension liquid is obtained and then introduced into the inside of the reaction kettle, the substrate is added, the reaction conditions are controlled, the material liquid after reaction is introduced into the inside of the first filtering device, the collected filtrate is introduced into the inside of the second filtering device, the collected filtrate is introduced into the first resin column, the collected effluent is introduced into the pH adjusting kettle, the effluent with appropriate pH is introduced into the second resin column, then the eluent in the eluent pipeline is used to elute the second resin column, the collected eluent is concentrated by the concentration kettle, the concentrated liquid is introduced into the first crystallization kettle for crystallization, the crystallized crystal is introduced into the first washing kettle for washing, the impurity-removed crystal is introduced into the decoloring kettle, the decoloring liquid after treatment by the decoloring agent is introduced into the second crystallization kettle, the crystal formed by cooling is introduced into the second washing kettle for further washing, the washed crystal is dried by the drying device, and finally the glycine storage tank is used for storage. The production system for preparation and purification can realize continuous production, greatly improves the yield, recovery rate and purity of glycine.

[0014] The first filter device is a ceramic membrane filter device with a pore size of 20-50 nm, which can effectively remove bacteria.

[0015] The second filter device is an ultrafiltration membrane filter device with a molecular weight cut-off of 5000-10000 Da, which can effectively remove large molecular proteins.

[0016] The first resin column is a cation resin column, and the second resin column is an anion resin column. The cation resin column can effectively remove metal ions, and the anion resin column can adsorb glycine.

[0017] The first crystallization kettle and the second crystallization kettle each include a kettle body, a feed inlet and a discharge outlet are arranged at the top and the bottom of the kettle body respectively, a crystallization mother liquor outlet is arranged at one side of the lower part of the kettle body, and a filter screen is arranged on the inner wall of the kettle body at a position corresponding to the crystallization mother liquor outlet; a jacket is arranged outside the kettle body, a stirring shaft is arranged inside the kettle body, one end of the stirring shaft is connected to a motor, and a plurality of stirring plates are arranged on the stirring shaft. The coolant in the jacket provides the temperature required for crystallization, the motor drives the stirring shaft and the plurality of stirring plates to rotate after being started, thereby achieving sufficient stirring of the material liquid and making the material liquid uniformly cooled, which greatly improves the crystallization efficiency; the crystallization mother liquor is discharged from the crystallization mother liquor outlet by passing through the filter screen under the action of a suction filter pump.

[0018] The first washing kettle and the second washing kettle each include a kettle body, a feed inlet and a washing liquid inlet are arranged at the top of the kettle body, a discharge outlet is arranged at the bottom of the kettle body, a washing liquid outlet is arranged at one side of the lower part of the kettle body, and a filter screen is arranged on the inner wall of the kettle body at a position corresponding to the washing liquid outlet; a rotating shaft is arranged inside the kettle body, one end of the rotating shaft is connected to a motor, a stirring frame is arranged on the rotating shaft, and a plurality of stirring rods are arranged on the stirring frame. The motor drives the rotating shaft, the stirring frame and the plurality of stirring rods to rotate after being started, so that the crystals and the washing liquid are in sufficient contact, which promotes the impurities in the crystals to dissolve in the washing liquid, and then the washing liquid is discharged from the washing liquid outlet by passing through the filter screen under the action of a suction filter pump, and the crystals are discharged from the discharge outlet. The above structure design is reasonable, realizes washing of the crystals, removes impurities, and improves the purity of the crystals.

[0019] The decoloring kettle comprises a kettle body, a feed inlet, a decoloring agent inlet and a solvent inlet are arranged at the top of the kettle body, a decoloring agent outlet is arranged at the bottom of the kettle body, a decoloring liquid outlet is arranged at one side of the lower part of the kettle body, a filter screen is arranged at the position corresponding to the decoloring liquid outlet on the inner wall of the kettle body, a jacket is arranged outside the kettle body, a stirring shaft is arranged inside the kettle body, one end of the stirring shaft is connected with a motor, and a plurality of stirring rods are arranged on the stirring shaft. In actual production, the glycine crude product is put into the kettle body, purified water is added, the heat medium in the jacket provides the temperature required for dissolving, the motor drives the stirring shaft and the plurality of stirring rods to rotate after being started, so that the glycine crude product is dissolved in water, then the decoloring agent is added and continues to be stirred, after the decoloring is completed, the decoloring liquid is discharged from the decoloring liquid outlet through the filter screen, and the decoloring agent is discharged from the decoloring agent outlet. The above-mentioned decoloring kettle has reasonable design, can effectively remove the pigment in the glycine crude product, and improves the color of the glycine. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is a structure schematic view of the production system for preparing and purifying glycine.

[0021] Among them, 1-seed tank, 2-fermentation tank, 3-centrifuge, 4-bacterial body storage tank, 5-buffer solution storage tank, 6-reaction kettle, 7-first filter device, 8-second filter device, 9-first resin column, 10-pH adjusting kettle, 11-second resin column, 12-eluent pipeline, 13-concentration kettle, 14-first crystallization kettle, 15-first washing kettle, 16-decoloring kettle, 17-second crystallization kettle, 18-second washing kettle, 19-drying device, 20-glycine storage tank. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical scheme and advantages of the utility model more clearly, the following will be further described in detail by combining with the drawings and examples. It should be understood that the specific examples described here are only used to explain the utility model, and are not used to limit the utility model.

[0023] A production system for preparing and purifying glycine, such as Figure 1As shown, it comprises a seed tank 1 (purchased from a manufacturer), the outlet of the seed tank 1 is connected to a fermentation tank 2 (purchased from a manufacturer), the outlet of the fermentation tank 2 is connected to a centrifuge 3 (disc centrifuge), the outlet of the centrifuge 3 is connected to a cell storage tank 4, the liquid inlet of the cell storage tank 4 is connected to a buffer storage tank 5, the outlet of the cell storage tank 4 is connected to a reaction kettle 6 (the top and bottom of the kettle body are respectively provided with an inlet and an outlet, the outside of the kettle body is provided with a jacket, the inside of the kettle body is provided with a stirring part, a temperature sensor and a pH sensor, and the temperature sensor and the pH sensor are respectively electrically connected to a controller), the outlet of the reaction kettle 6 is connected to a first filtering device 7 (ceramic membrane filtering equipment with a pore size of 20-50 nm), the outlet of the first filtering device 7 is connected to a second filtering device 8 (ultrafiltration membrane filtering equipment with a molecular weight cut-off of 5000-10000 Da), the outlet of the second filtering device 8 is connected to a first resin column 9 (a cation resin column), the effluent outlet of the first resin column is connected to a pH adjusting kettle 10, the outlet of the pH adjusting kettle 10 is connected to a second resin column 11 (an anion resin column), the liquid inlet of the second resin column 11 is connected to an eluent pipeline 12, the eluent outlet of the second resin column 11 is connected to a concentration kettle 13 (the top and bottom of the kettle body are respectively provided with an inlet and a concentrated liquid outlet, the outside is provided with a jacket, and the inside is provided with a stirring part driven by a motor), the outlet of the concentration kettle 13 is connected to a first crystallization kettle 14, the crystal outlet of the first crystallization kettle 14 is connected to a first washing kettle 15, the outlet of the first washing kettle 15 is connected to a decolorization kettle 16, the decolorization liquid outlet of the decolorization kettle 16 is connected to a second crystallization kettle 17, the crystal outlet of the second crystallization kettle 17 is connected to a second washing kettle 18, the outlet of the second washing kettle 18 is connected to a drying device 19 (a double-cone dryer), and the outlet of the drying device 19 is connected to a glycine storage tank 20.

[0024] In actual production, the medium, substrate and other components are put into the seed tank, the culture conditions are controlled, the bacteria after culture are transferred into the fermentation tank containing fermentation medium, the fermentation conditions are controlled, the fermentation liquor after fermentation is separated by centrifuge, the collected bacteria are transferred into the bacteria storage tank, at the same time, the buffer in the buffer storage tank is transferred into the bacteria storage tank, the bacteria resuspension is obtained and then is transferred into the inside of the reaction kettle, the substrate is added, the reaction conditions are controlled, the material liquid after reaction is transferred into the inside of the first filter device, the collected filtrate is transferred into the inside of the second filter device, the collected filtrate is transferred into the first resin column (absorbing metal ions), the collected effluent is transferred into the pH adjusting kettle, the effluent with suitable pH is transferred into the second resin column (absorbing glycine), then the eluent in the eluent pipeline is used to elute the second resin column, the collected eluent is concentrated by the concentration kettle, the concentrated liquid is transferred into the first crystallization kettle for crystallization, the crystals after crystallization are transferred into the first washing kettle for washing, the impurities in the crystals are removed, the crystals after removal of impurities are transferred into the decolorization kettle, the decolorization liquid after treatment by the decolorization agent is transferred into the second crystallization kettle, the crystals after temperature reduction are transferred into the second washing kettle for further washing, the washed crystals are dried by the drying device, and finally the glycine storage tank is used for storage. The production system for preparation and purification can realize continuous production, and greatly improves the yield, recovery and purity of glycine.

[0025] The first crystallization kettle 14 and the second crystallization kettle 17 each include a kettle body, the top and bottom of the kettle body are respectively provided with a feed inlet and a discharge outlet, one side of the lower part of the kettle body is provided with a crystallization mother liquor outlet, and the inner wall of the kettle body is provided with a filter screen at a position corresponding to the crystallization mother liquor outlet; the outside of the kettle body is provided with a jacket, the inside of the kettle body is provided with a stirring shaft, one end of the stirring shaft is connected with a motor, and the stirring shaft is provided with multiple layers of stirring blades. The refrigerant in the jacket provides the temperature required for crystallization, the motor drives the stirring shaft and the multiple layers of stirring blades to rotate after being started, thereby realizing sufficient stirring of the material liquid, making the material liquid uniformly cooled, greatly improving the crystallization efficiency, and the crystallization mother liquor is discharged from the crystallization mother liquor outlet under the action of a suction filter pump and passes through the filter screen.

[0026] The first washing kettle 15 and the second washing kettle 18 each include a kettle body, the top of the kettle body is provided with a feed inlet and a washing liquid inlet, the bottom of the kettle body is provided with a discharge outlet, one side of the lower part of the kettle body is provided with a washing liquid outlet, and the inner wall of the kettle body is provided with a filter screen at a position corresponding to the washing liquid outlet; the inside of the kettle body is provided with a rotating shaft, one end of the rotating shaft is connected with a motor, the rotating shaft is provided with a stirring frame, and the stirring frame is provided with multiple stirring rods. The motor drives the rotating shaft, the stirring frame and the multiple stirring rods to rotate after being started, so that the crystals and the washing liquid are in sufficient contact, thereby promoting the impurities in the crystals to dissolve in the washing liquid, then the washing liquid passes through the filter screen from the washing liquid outlet under the action of a suction filter pump, and the crystals are discharged from the discharge outlet. The above structure design is reasonable, realizes washing of the crystals, removal of impurities and improvement of the purity of the crystals.

[0027] The decoloring kettle 16 comprises a kettle body, a feed inlet, a decoloring agent inlet and a solvent inlet are arranged at the top of the kettle body, a decoloring agent outlet is arranged at the bottom of the kettle body, a decoloring liquid outlet is arranged at one side of the lower part of the kettle body, a filter screen is arranged at the position corresponding to the decoloring liquid outlet on the inner wall of the kettle body, a jacket is arranged outside the kettle body, a stirring shaft is arranged inside the kettle body, the one end of the stirring shaft is connected with a motor, and a plurality of stirring rods are arranged on the stirring shaft. In actual production, the crude glycine is put into the kettle body, purified water is added, the heat medium in the jacket provides the temperature required for dissolving, the motor drives the stirring shaft and the plurality of stirring rods to rotate after being started, the crude glycine is dissolved in water, then the decoloring agent is added and continues to be stirred, after the decoloring is completed, the decoloring liquid passes through the filter screen and is discharged from the decoloring liquid outlet, and the decoloring agent is discharged from the decoloring agent outlet. The above decoloring kettle has reasonable design, can effectively remove the pigment in the crude glycine, and improves the color of the glycine.

[0028] The above merely describes the preferred embodiments of the present application and is not used to limit the present application, and any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A production system for preparing and purifying glycine, characterized in that: The invention comprises a seed tank, the outlet of the seed tank is connected to a fermentation tank, the outlet of the fermentation tank is connected to a centrifuge, the discharge port of the centrifuge is connected to a bacterial cell storage tank, the liquid inlet of the bacterial cell storage tank is connected to a buffer storage tank, the discharge port of the bacterial cell storage tank is connected to a reactor, the outlet of the reactor is connected to a first filter device, the outlet of the first filter device is connected to a second filter device, the outlet of the second filter device is connected to a first resin column, the effluent outlet of the first resin column is connected to a pH regulating kettle, the discharge port of the pH regulating kettle is connected to a second resin column, the liquid inlet of the second resin column is connected to an eluent pipeline, the eluate outlet of the second resin column is connected to a concentration kettle, the liquid outlet of the concentration kettle is connected to a first crystallization kettle, the crystal outlet of the first crystallization kettle is connected to a first washing kettle, the discharge port of the first washing kettle is connected to a decolorization kettle, the decolorant outlet of the decolorization kettle is connected to a second crystallization kettle, the crystal outlet of the second crystallization kettle is connected to a second washing kettle, the discharge port of the second washing kettle is connected to a drying device, and the outlet of the drying device is connected to a glycine storage tank.

2. A production system for preparing and purifying glycine according to claim 1, characterized in that: The first filtering device is a ceramic membrane filtering device with a pore size of 20-50nm.

3. A production system for preparing and purifying glycine according to claim 1, characterized in that: The second filtering device is an ultrafiltration membrane filtering device with a molecular weight cut-off of 5000-10000Da.

4. A production system for preparing and purifying glycine according to claim 1, characterized in that: The first resin column is a cationic resin column, and the second resin column is an anionic resin column.

5. The production system for preparing and purifying glycine according to claim 1, characterized in that: The first crystallization kettle and the second crystallization kettle both include a kettle body, wherein the top and bottom of the kettle body are respectively provided with a feed port and a discharge port, a crystallization mother liquor outlet is provided on one side of the lower part of the kettle body, and a filter is provided on the inner wall of the kettle body at a position corresponding to the crystallization mother liquor outlet; a jacket is provided on the outside of the kettle body, and a stirring shaft is provided on the inside of the kettle body, one end of the stirring shaft is connected to a motor, and a multi-layer stirring plate is provided on the stirring shaft.

6. A production system for preparing and purifying glycine according to claim 1, characterized in that: The first washing kettle and the second washing kettle both include a kettle body, a feed port and a washing liquid inlet are provided on the top of the kettle body, a discharge port is provided on the bottom of the kettle body, a washing liquid outlet is provided on one side of the lower part of the kettle body, and a filter is provided on the inner wall of the kettle body at a position corresponding to the washing liquid outlet; a rotating shaft is provided inside the kettle body, one end of the rotating shaft is connected to a motor, a stirring frame is provided on the rotating shaft, and a plurality of stirring rods are provided on the stirring frame.

7. A production system for preparing and purifying glycine according to claim 1, characterized in that: The decolorization kettle includes a kettle body, a feed inlet, a decolorant inlet and a solvent inlet are provided on the top of the kettle body, a decolorant outlet is provided at the bottom of the kettle body, a decolorant outlet is provided on one side of the lower part of the kettle body, and a filter is provided on the inner wall of the kettle body at a position corresponding to the decolorant outlet; a jacket is provided on the outside of the kettle body, and a stirring shaft is provided on the inside of the kettle body, one end of the stirring shaft is connected to a motor, and a plurality of stirring rods are provided on the stirring shaft.

Images