Polysaccharide determination equipment for ganoderma lucidum capsule

Abstract

The polysaccharide measuring equipment comprises a table top, a supporting frame is installed on the lower end face of the table top, a hydrolysis box is installed on one side of the upper end face of the table top, a rotating motor is installed on the upper end face of the hydrolysis box, a stirring shaft is arranged below the rotating motor, and a stirring shaft is arranged below the stirring shaft. A plurality of rotating paddles are uniformly mounted on the outer surface of the stirring shaft, a feeding pipe is arranged on one side of the rotating motor, a dialysis separation box is mounted on one side of the hydrolysis box, a water pump is mounted on one side of the outer surface of the dialysis separation box, and the water pump is connected with the hydrolysis box through a first water pipe; an electrode plate is mounted on one side of the interior of the dialysis separation box, and an ion exchange device is movably mounted on the other side of the interior of the dialysis separation box through a detachable mechanism. The device is free of interference, the ion exchange membrane can be replaced, the subsequent glucose separation effect is guaranteed, and the accuracy of the polysaccharide determination result of the ganoderma lucidum capsule is high.

Description

TECHNICAL FIELD

[0001] The utility model relates to ganoderma lucidum capsule polysaccharide determination technical field, specifically a kind of polysaccharide determination equipment of ganoderma lucidum capsule. BACKGROUND

[0002] Ganoderma lucidum capsule is a common traditional Chinese medicinal material preparation, has multiple effects and functions, such as immune regulation, anti-tumor, antioxidant, cardiovascular protection, nervous system regulation, liver protection. The standard for ganoderma lucidum capsule is carried out by Ministry of Public Health Chinese medicine formula preparation standard, and the standard does not have the determination of ganoderma lucidum capsule polysaccharide content, and other traditional Chinese medicine polysaccharide determination in China mostly uses ultraviolet spectrophotometer determination, and the polysaccharide content measured according to wavelength is the total polysaccharide content. In the production process of ganoderma lucidum capsule, starch filling is added, and when using ultraviolet spectrophotometer to determine polysaccharide, the polysaccharide in starch is also measured. Therefore, when determining the polysaccharide content of ganoderma lucidum capsule, starch needs to be separated out, and then high performance liquid chromatography is used to determine the polysaccharide content, to increase the accuracy of determination results.

[0003] However, the existing polysaccharide determination technology is ultraviolet spectrophotometry, which hydrolyzes polysaccharide into monosaccharide under the action of concentrated sulfuric acid and rapidly dehydrates to generate glycoform and its derivatives, and develops color with anthrone reagent, and the color depth and ganoderma lucidum polysaccharide content show linear relationship, this method is not applicable to the determination of polysaccharide of ganoderma lucidum capsule containing starch, and the determination result error is large, and the operation is also relatively cumbersome, therefore, it does not meet the existing demand, and for this, we propose a polysaccharide determination equipment of ganoderma lucidum capsule. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of polysaccharide determination equipment of ganoderma lucidum capsule, to solve the existing polysaccharide determination technology as ultraviolet spectrophotometry in the background art proposed, which hydrolyzes polysaccharide into monosaccharide under the action of concentrated sulfuric acid and rapidly dehydrates to generate glycoform and its derivatives, and develops color with anthrone reagent, and the color depth and ganoderma lucidum polysaccharide content show linear relationship, this method is not applicable to the determination of polysaccharide of ganoderma lucidum capsule containing starch, and the determination result error is large, and the operation is also relatively cumbersome, etc.

[0005] In order to achieve the above object, the utility model provides the following technical scheme: A kind of polysaccharide determination equipment of ganoderma lucidum capsule, including desktop, the lower end surface of the desktop is equipped with support frame, the side of the upper end surface of the desktop is equipped with hydrolysis box, the upper end surface of the hydrolysis box is equipped with rotating motor, the lower of the rotating motor is provided with stirring shaft, the outer surface of the stirring shaft is evenly equipped with multiple rotating propellers, the side of the rotating motor is provided with feeding pipe, one side of the hydrolysis box is equipped with dialysis separation tank, the side of the outer surface of the dialysis separation tank is equipped with water pump, the water pump is connected with hydrolysis box by first water pipe, one side of the inside of the dialysis separation tank is equipped with electrode plate, the other side of the inside of the dialysis separation tank is movably equipped with ion exchange device by detachable mechanism, one side of the dialysis separation tank is equipped with high performance liquid chromatograph, the side of the outer surface of the high performance liquid chromatograph is equipped with metering pump, the metering pump is connected with dialysis separation tank by second water pipe.

[0006] Preferably, the output end of the rotating motor is fixedly connected with the upper end surface of the stirring shaft through a shaft coupling, the lower end surface of the stirring shaft is rotatably connected with the inner surface of the hydrolysis box through a bearing, and the lower end surface of the feeding pipe extends into the hydrolysis box.

[0007] Preferably, the inside of the first water pipe is equipped with a first electromagnetic valve, the other end of the water pump is connected with the inside of the dialysis separation tank through a first connecting pipe, the other end of the metering pump is connected with the sample inlet of the high performance liquid chromatograph through a second connecting pipe, and the inside of the second water pipe is equipped with a second electromagnetic valve.

[0008] Preferably, the ion exchange device comprises a back-shaped frame, the inside of the back-shaped frame is fixedly connected with an ion exchange membrane, the detachable mechanism comprises a vertical plate, the vertical plate is arranged on the front end surface and the rear end surface of one side of the inner wall of the dialysis separation tank through a vertical groove, and the outer surface of the back-shaped frame is fixedly connected with the outer surface of the vertical plate.

[0009] Preferably, the upper side of the outer surface of the two vertical plates is provided with a jack, the upper side of the front end surface and the rear end surface of the dialysis separation tank is provided with a through hole, the inside of the through hole is provided with a plug rod, the outer surface of the plug rod is sleeved with a second spring, and the plug rod can be clamped into the inside of the jack.

[0010] Preferably, the bottom surface of the vertical groove is provided with a groove, the bottom surface of the groove is equipped with a first spring, and the elastic force of the two first springs is greater than the sum of the gravity of the two vertical plates and the ion exchange device.

[0011] Compared with the prior art, the utility model has the beneficial effects that:

[0012] 1. The utility model discloses a hydrolysis tank, water and powder in ganoderma lucidum capsule are added to the hydrolysis tank through the feeding pipe, and then hydrolytic enzyme is added, the switch of rotary motor is opened, the stirring shaft rotates, drives the rotating propeller to stir, makes it mixed hydrolysis even, the switch of water pump is opened, and the solution after hydrolysis is transported to the dialysis separation tank, and the solution will be separated and extracted under the joint action of ion exchange membrane and electrode plate, and the separated solution enters the high performance liquid chromatograph under the action of metering pump and is detected, the utility model discloses no interference, and the determination result accuracy of polysaccharide determination of ganoderma lucidum capsule is high,

[0013] 2. The utility model discloses a detachable mechanism, convenient for the dismounting of ion exchange device, thereby facilitating the replacement of ion exchange membrane, can simultaneously pull out two insertion rods, the other end of insertion rod leaves the inner side of insertion hole, and the limiting of vertical plate is released, and the vertical plate and ion exchange device are jacked up under the action of first spring, convenient for staff to dismount the vertical plate and ion exchange device from the inside of dialysis separation tank, replace ion exchange membrane, guarantee the effect of subsequent glucose separation, and guarantee the accuracy of determination result. ACCURACY

[0014] Fig. 1 It is the overall structure schematic view of the utility model;

[0015] Fig. 2 It is the main sectional view of the utility model whole;

[0016] Fig. 3 It is the structure schematic view of the detachable mechanism of the utility model.

[0017] In the drawing: 1, desktop; 2, high performance liquid chromatograph; 3, dialysis separation tank; 4, ion exchange device; 5, electrode plate; 6, rotary motor; 7, hydrolysis tank; 8, first water pipe; 9, water pump; 10, second water pipe; 11, metering pump; 12, feeding pipe; 13, rotating propeller; 14, stirring shaft; 15, detachable mechanism; 1501, vertical plate; 1502, vertical groove; 1503, first spring; 1504, recess; 1505, insertion rod; 1506, second spring; 1507, insertion hole; 1508, through -hole. DETAILED DESCRIPTION

[0018] The technical scheme in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model, and apparently, the described embodiments only are a part of the embodiments of the utility model, not all the embodiments.

[0019] The rotary motor 6 (model number YEJ3-112M-4), the water pump 9 (model number IS200-150-400) and the metering pump 11 (model number GB-S) mentioned in the utility model can be obtained by market purchase or private customization.

[0020] Please refer to Figs. 1 to 3 The utility model provides a kind of polysaccharide determination equipment of ganoderma lucidum capsule, including desktop 1, the lower end surface of desktop 1 is installed with support frame, the upper end surface of desktop 1 one side is installed with hydrolytic tank 7, the upper end surface of hydrolytic tank 7 is installed with rotary motor 6, the lower of rotary motor 6 is provided with stirring shaft 14, the outer surface of stirring shaft 14 is evenly installed with multiple rotating paddle 13, one side of rotary motor 6 is provided with feeding pipe 12, one side of hydrolytic tank 7 is installed with dialysis separation tank 3, one side of the outer surface of dialysis separation tank 3 is installed with water pump 9, water pump 9 is connected with hydrolytic tank 7 by first water pipe 8, one side of the inside of dialysis separation tank 3 is installed with electrode plate 5, the other side of the inside of dialysis separation tank 3 is movably installed with ion exchange device 4 by detachable mechanism 15, one side of dialysis separation tank 3 is installed with high performance liquid chromatograph 2, one side of the outer surface of high performance liquid chromatograph 2 is installed with metering pump 11, metering pump 11 is connected with dialysis separation tank 3 by second water pipe 10.

[0021] The output end of rotary motor 6 is fixedly connected with the upper end surface of stirring shaft 14 by coupling, the lower end surface of stirring shaft 14 is rotatably connected with the inner surface of hydrolytic tank 7 by bearing, the lower end surface of feeding pipe 12 extends to the inside of hydrolytic tank 7, rotary motor 6 can drive stirring shaft 14 to rotate when working, and the powder and hydrolytic enzyme in ganoderma lucidum capsule can be added into hydrolytic tank 7 through feeding pipe 12.

[0022] The inside of first water pipe 8 is installed with first electromagnetic valve, the other end of water pump 9 is connected with the inside of dialysis separation tank 3 by first connecting pipe, the other end of metering pump 11 is connected with the sample inlet of high performance liquid chromatograph 2 by second connecting pipe, the inside of second water pipe 10 is installed with second electromagnetic valve, water pump 9 can transport the liquid in hydrolytic tank 7 to dialysis separation tank 3 when working, and metering pump 11 can quantitatively transport the liquid in dialysis separation tank 3 to high performance liquid chromatograph 2 when working.

[0023] Ion exchange device 4 includes back type frame, back type frame is fixedly connected with ion exchange membrane on the inside, detachable mechanism 15 includes vertical plate 1501, vertical plate 1501 is arranged on the front end surface and rear end surface of one side of the inner wall of dialysis separation tank 3 through vertical groove 1502, the outer surface of back type frame is fixedly connected with the outer surface of vertical plate 1501.

[0024] The upper part of the outer surface of the two vertical plates 1501 is provided with a jack 1507, the upper part of the front end surface and the rear end surface of the dialysis separation tank 3 is provided with a through hole 1508, the inner side of the through hole 1508 is provided with a plug rod 1505, the outer surface of the plug rod 1505 is sleeved with a second spring 1506, and the plug rod 1505 can be clamped into the inner side of the jack 1507, so as to limit the vertical plate 1501.

[0025] The bottom surface of the vertical groove 1502 is provided with a groove 1504, the bottom surface of the groove 1504 is provided with a first spring 1503, the elastic force of the two first springs 1503 is greater than the sum of the weight of the two vertical plates 1501 and the ion exchange device 4, the first spring 1503 has an upward pushing force on the vertical plate 1501 and the ion exchange device 4, when the plug rod 1505 is away from the inner side of the jack 1507, the first spring 1503 will push the vertical plate 1501 and the ion exchange device 4 upward, so as to facilitate the worker to take out the vertical plate 1501 and the ion exchange device 4.

[0026] When the polysaccharide determination equipment of the ganoderma lucidum capsule is used, first, the power supply is turned on, the high performance liquid chromatograph 2, the dialysis separation tank 3 and the hydrolysis tank 7 are arranged, the water and the powder in the ganoderma lucidum capsule are added into the hydrolysis tank 7 through the feeding pipe 12, the hydrolysis enzyme is added, the switch of the rotating motor 6 is turned on, the stirring shaft 14 is rotated, the rotating paddle 13 is driven to stir, the mixture is uniformly hydrolyzed, the switch of the water pump 9 is turned on, the hydrolyzed solution is transported into the dialysis separation tank 3, the solution is separated and extracted under the joint action of the ion exchange membrane and the electrode plate 5, the starch-decomposed glucose is separated, the separated solution enters the high performance liquid chromatograph 2 under the action of the metering pump 11, the ion exchange device 4 is conveniently disassembled and assembled through the detachable mechanism 15, so as to conveniently replace the ion exchange membrane, the two plug rods 1505 can be pulled outwards at the same time, the other end of the plug rod 1505 is away from the inner side of the jack 1507, the limiting of the vertical plate 1501 is released, the vertical plate 1501 and the ion exchange device 4 are pushed upward under the action of the first spring 1503, so as to facilitate the worker to disassemble the vertical plate 1501 and the ion exchange device 4 from the inner side of the dialysis separation tank 3, the utility model has no interference, the ion exchange membrane can be replaced, the effect of subsequent separation of glucose is guaranteed, the determination result of the polysaccharide determination of the ganoderma lucidum capsule is high in accuracy.

[0027] It is apparent for a person skilled in the art that the present application is not restricted to the details of the above exemplary embodiments, but that it can be implemented in other concrete forms without departing from the spirit or the essential characteristics of the present application. Therefore, the embodiments should be considered as exemplary only, and not limiting, the scope of the present application being defined by the appended claims rather than the above description, and all changes coming within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference signs in the claims should not be construed as limiting the claims concerned.

Claims

1. A ganoderma polysaccharide capsule measuring device, comprising a desktop (1), the lower end face of the desktop (1) is provided with a support frame, characterized in that: The upper end face of the desktop (1) is provided with a hydrolysis tank (7), the upper end face of the hydrolysis tank (7) is provided with a rotary motor (6), the lower end of the rotary motor (6) is provided with a stirring shaft (14), the outer surface of the stirring shaft (14) is uniformly provided with a plurality of rotating paddles (13), one side of the rotary motor (6) is provided with a feeding pipe (12), one side of the hydrolysis tank (7) is provided with a dialysis separation tank (3), one side of the outer surface of the dialysis separation tank (3) is provided with a water pump (9), the water pump (9) is connected with the hydrolysis tank (7) through a first water pipe (8), one side of the inside of the dialysis separation tank (3) is provided with an electrode plate (5), the other side of the inside of the dialysis separation tank (3) is movably provided with an ion exchange device (4) through a detachable mechanism (15), one side of the dialysis separation tank (3) is provided with a high performance liquid chromatograph (2), one side of the outer surface of the high performance liquid chromatograph (2) is provided with a metering pump (11), the metering pump (11) is connected with the dialysis separation tank (3) through a second water pipe (10).

2. A device for measuring polysaccharides of ganoderma lucidum capsules according to claim 1, characterized in that: The output end of the rotary motor (6) is fixedly connected with the upper end face of the stirring shaft (14) through a shaft coupling, the lower end face of the stirring shaft (14) is rotatably connected with the inner surface of the hydrolysis tank (7) through a bearing, and the lower end face of the feeding pipe (12) extends into the inside of the hydrolysis tank (7).

3. The ganoderma capsule polysaccharide determination device according to claim 1, characterized in that: A first electromagnetic valve is mounted on the inner side of the first water pipe (8), the other end of the water pump (9) is connected with the inside of the dialysis separation tank (3) through a first connecting pipe, the other end of the metering pump (11) is connected with the sample inlet of the high performance liquid chromatograph (2) through a second connecting pipe, and a second electromagnetic valve is mounted on the inner side of the second water pipe (10).

4. The ganoderma capsule polysaccharide measuring device according to claim 1, characterized in that: The ion exchange device (4) comprises a back-shaped frame, the inner side of the back-shaped frame is fixedly connected with an ion exchange membrane, the detachable mechanism (15) comprises a vertical plate (1501), the vertical plate (1501) is arranged on the front end face and the rear end face of one side of the inner wall of the dialysis separation tank (3) through a vertical groove (1502), and the outer surface of the back-shaped frame is fixedly connected with the outer surface of the vertical plate (1501).

5. A device for measuring polysaccharides of Ganoderma lucidum capsules according to claim 4, characterized in that: The upper end of the outer surface of each of the two vertical plates (1501) is provided with a jack (1507), the upper end of the front end face and the rear end face of the dialysis separation tank (3) is provided with a through hole (1508), the inner side of the through hole (1508) is provided with a plug rod (1505), the outer surface of the plug rod (1505) is sleeved with a second spring (1506), and the plug rod (1505) can be clamped into the inner side of the jack (1507).

6. A device for measuring polysaccharide of ganoderma lucidum capsule according to claim 4, characterized in that: The bottom surface of the vertical groove (1502) is provided with a groove (1504), the bottom surface of the groove (1504) is provided with a first spring (1503), and the elastic force of the two first springs (1503) is greater than the sum of the gravity of the two vertical plates (1501) and the ion exchange device (4).

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