Plate frame for injection
By designing an injection plate holder, the problem of controlling the injection depth and angle in three-dimensional cell culture using the injection method was solved, achieving precise control of the injection depth and angle and improving experimental efficiency.
Patent Information
- Application Number
- CN202422732021.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-11
- Publication Date
- 2025-11-04
- Estimated Expiration
- 2034-11-11
AI Technical Summary
In existing technologies, it is difficult to precisely control the injection depth and angle during three-dimensional cell culture using injection methods, resulting in high operational difficulty and limited efficiency.
An injection plate holder was designed, comprising a main frame, support plates, limiting grooves, reinforcing ribs, limiting mechanisms, and locking mechanisms. Through the cooperation of these components, the pipette tip is ensured to be inserted vertically into the center of the gel, achieving precise control of injection depth and angle.
It improves the experimental efficiency of three-dimensional cell culture by injection, ensures the stability of injection depth and angle, has a wide range of applications, and is suitable for different injection depth requirements.
Smart Images

Figure CN223509857U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the field of cell culture plate and frame technology, and in particular relates to an injection plate and frame. Background Technology
[0002] The injection method is a three-dimensional cell culture technique that uses a pipette to inject a cell suspension into a gel in a cell culture plate. The injection method involves three main variables: injection depth, injection angle, and injection speed. Holding the pipette tip directly into the gel makes precise control of injection depth and angle difficult, resulting in a challenging operation and limiting the efficiency of three-dimensional cell culture using this method. Utility Model Content
[0003] In view of the above-mentioned shortcomings of the prior art, the present invention provides an injection plate holder, which can effectively solve the problems of the prior art.
[0004] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:
[0005] This utility model is an injection plate holder, including a main frame and support leg plates evenly fixed to the lower end of the main frame, and further including:
[0006] A limiting slide is provided on the upper surface of the main frame, and a frame plate is engaged on the inner side of the limiting slide. The frame plate has evenly distributed round holes inside.
[0007] A limiting mechanism, located on the inner wall of the limiting groove, is used to limit the position of the shelf plate;
[0008] The locking mechanism is located on the left and right sides of the upper end face of the main frame and is used to lock the frame plates.
[0009] Furthermore, the left and right sides of the support leg plate are provided with reinforcing ribs, and the reinforcing ribs are arc-shaped structures that contact the lower end face of the main frame.
[0010] Furthermore, a reinforcing ring plate is fixed to the inner wall of the defined slide groove, and the reinforcing ring plate has an arc-shaped structure and is engaged with the frame plate.
[0011] Furthermore, the limiting mechanism includes a reinforcing plate and a reinforcing groove. The reinforcing plate is evenly fixed on the left and right sides of the frame plate, and the reinforcing groove is evenly opened on the upper end surface of the main frame. The reinforcing plate is slidably embedded in the reinforcing plate.
[0012] Furthermore, the locking mechanism includes a locking clip, a locking rod, and a torsion spring. The locking clip is symmetrically arranged on the left and right sides of the upper end face of the main frame. The locking rod is connected to the lower end face of the locking clip and is rotatably connected inside the main frame. The torsion spring is sleeved on the outer surface of the locking rod and is located inside the main frame.
[0013] This utility model has the following beneficial effects:
[0014] This invention features a support leg plate that supports the main frame, while reinforcing ribs strengthen the support leg plate, preventing it from shifting or wobbling. The support leg plate can also hold cell culture plates. The frame plate is then embedded in a defined groove and reinforced by a reinforcing ring and limiting mechanism to prevent shifting. The frame plate can be quickly disassembled, allowing for selection of frame plates with different diameter holes to suit different injection depths, thus expanding the applicability of the equipment. Finally, a locking mechanism secures the frame plate, preventing it from wobbling during use.
[0015] This invention features a pipette tip that is inserted into the gel through a circular hole in a holder. Based on the overall height of the holder, the pipette tip passes vertically through the hole and is inserted into the exact center of the gel, ensuring that the injection depth and angle remain constant. Then, the cell suspension can be injected slowly and at a uniform speed. This design also further standardizes the injection speed and greatly improves the overall experimental efficiency. Attached Figure Description
[0016] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0017] Figure 1 This is a top view of the injection plate holder of this utility model;
[0018] Figure 2 This is a bottom view of the injection plate holder of this utility model;
[0019] Figure 3 This is a top view of the main frame of this utility model;
[0020] Figure 4 This is a bottom view of the frame of this utility model;
[0021] Figure 5 This is a top view of the locking mechanism of this utility model.
[0022] The attached diagram lists the components represented by each number as follows:
[0023] 1. Main frame; 2. Support leg plate; 3. Reinforcing rib; 4. Limiting groove; 5. Reinforcing ring plate; 6. Frame plate; 7. Circular hole body; 8. Reinforcing plate; 9. Reinforcing groove; 10. Locking clip; 11. Locking rod; 12. Torsion spring. Detailed Implementation
[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.
[0025] Please see Figure 1-5 As shown, this utility model is an injection plate holder, including a main frame 1 and support leg plates 2 evenly fixed to the lower end of the main frame 1, and further including:
[0026] A limiting groove 4 is provided on the upper end face of the main frame 1, and a frame plate 6 is engaged on the inner side of the limiting groove 4. The frame plate 6 has evenly distributed round holes 7 inside. Reinforcing ribs 3 are provided on the left and right sides of the support leg plate 2. The reinforcing ribs 3 have an arc-shaped structure and are in contact with the lower end face of the main frame 1. The support leg plate 2 can support the main frame 1, and the reinforcing ribs 3 can strengthen the support leg plate 2, which can prevent the support leg plate 2 from shifting or shaking.
[0027] A limiting mechanism is installed on the inner wall of the limiting groove 4 to limit the position of the frame plate 6. A reinforcing ring plate 5 is fixed to the inner wall of the limiting groove 4. The reinforcing ring plate 5 has an arc-shaped structure and is engaged with the frame plate 6. The limiting groove 4 and the reinforcing ring plate 5 are set in a stepped manner to limit the frame plate 6 and prevent the frame plate 6 from shifting. The limiting mechanism includes a reinforcing plate 8 and a reinforcing groove 9. The reinforcing plate 8 is evenly fixed on the left and right sides of the frame plate 6. The reinforcing groove 9 is evenly opened on the upper end face of the main frame 1. The reinforcing plate 8 is slidably embedded in the reinforcing plate 8. The frame plate 6 can be embedded in the limiting groove 4. The reinforcing ring plate 5 and the limiting mechanism reinforce and limit the frame plate 6 to prevent the frame plate 6 from shifting. At the same time, since the frame plate 6 can be quickly disassembled, different round hole bodies 7 of the frame plate 6 can be selected according to different injection depths, so that different specifications of frame plates 6 can be selected as needed, which can further improve the applicability of the equipment.
[0028] A locking mechanism is installed on the left and right sides of the upper end face of the main frame 1 to lock the shelf plate 6. The locking mechanism includes a locking clip 10, a locking rod 11 and a torsion spring 12. The locking clip 10 is symmetrically arranged on the left and right sides of the upper end face of the main frame 1. The locking rod 11 is connected to the lower end face of the locking clip 10 and is rotatably connected to the inside of the main frame 1. The torsion spring 12 is sleeved on the outer surface of the locking rod 11 and is located inside the main frame 1. The torsion spring 12 can reset the locking rod 11. The upper end of the locking rod 11 is connected to the locking clip 10, so the angle of the locking clip 10 can be adjusted so that the locking clip 10 is locked and limited to the shelf plate 6, which can prevent the shelf plate 6 from shaking during use.
[0029] Working principle: In terms of design, the circular holes 7 on the holder 6 correspond one-to-one with the holes in the cell culture plate to be injected. After the pipette tip is inserted into the circular hole 7 on the holder 6, the upper end of the pipette tip is locked in place by the circular hole 7, which can simultaneously limit the height and angle of the pipette tip. Then, the pipette tip is inserted into the gel in the cell culture plate through the circular hole 7. According to the overall height of the plate holder, the pipette tip is inserted vertically into the exact center of the gel in the lower circular hole 7, thus ensuring that the injection depth and angle remain unchanged. Then, the cell suspension is slowly and uniformly injected. In addition, this also makes it possible to use a 10ul multi-channel pipette for batch injection of three-dimensional cell culture, which can further unify the injection speed and greatly improve the overall experimental efficiency. The injection results obtained by this method are not significantly different from those obtained by hand injection.
[0030] The above are merely preferred embodiments of the present utility model and do not limit the present utility model. Any modifications, equivalent substitutions, or improvements made to the technical solutions described in the foregoing embodiments, or to some of the technical features, shall fall within the protection scope of the present utility model.
Claims
1. An injection plate holder, comprising a main frame (1) and support plates (2) uniformly fixed to the lower end of the main frame (1), characterized in that, Also includes: A limiting groove (4) is opened on the upper end face of the main frame (1), and a frame plate (6) is snapped into the inner side of the limiting groove (4). Circular holes (7) are evenly opened inside the frame plate (6). A limiting mechanism is provided on the inner wall of the limiting slide (4) to limit the position of the shelf (6); The locking mechanism is set on the left and right sides of the upper end face of the main frame (1) and is used to lock the frame plate (6).
2. The injection plate holder according to claim 1, characterized in that, The support leg plate (2) is provided with reinforcing ribs (3) on the left and right sides, and the reinforcing ribs (3) are arc-shaped structures and are in contact with the lower end face of the main frame (1).
3. The injection plate holder according to claim 1, characterized in that, The inner wall of the defined slide groove (4) is fixed with a reinforcing ring plate (5), and the reinforcing ring plate (5) has an arc-shaped structure and is engaged with the frame plate (6).
4. The injection plate holder according to claim 3, characterized in that, The limiting mechanism includes a reinforcing plate (8) and a reinforcing groove (9). The reinforcing plate (8) is evenly fixed on the left and right sides of the frame plate (6), and the reinforcing groove (9) is evenly opened on the upper surface of the main frame (1). The reinforcing plate (8) is slidably embedded in the reinforcing plate (8).
5. The injection plate holder according to claim 1, characterized in that, The locking mechanism includes a locking clip (10), a locking rod (11), and a torsion spring (12). The locking clip (10) is symmetrically arranged on the left and right sides of the upper end face of the main frame (1). The locking rod (11) is connected to the lower end face of the locking clip (10) and is rotatably connected to the inside of the main frame (1). The torsion spring (12) is sleeved on the outer surface of the locking rod (11) and is located inside the main frame (1).