Outdoor portable LAMP-CRISPR detection result observation device

Abstract

The utility model relates to an outdoor portable LAMP-CRISPR detection result observation device which comprises a container used for containing a LAMP-CRISPR sample to be detected, and a light source used for providing blue light for observation of the LAMP-CRISPR sample is arranged on the container; and a yellow light film for observing the LAMP-CRISPR sample is also arranged on the wall of the container. The device disclosed by the utility model is designed on the basis of fluorescent dye in combination with a naked eye observation method, is small in size, light in weight and high in convenience, and can be used in various outdoor environments.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of experimental devices, in particular to a device for observing the detection results of outdoor portable LAMP-CRISPR. BACKGROUND

[0002] In LAMP-CRISPR detection, some fluorescent dyes are added to the LAMP-CRISPR reaction system, such as a probe with a fluorescent ligand that specifically recognizes the target nucleic acid sequence in the LAMP-CRISPR reaction. Taking the TaqMan probe as an example, during the LAMP-CRISPR reaction, when the primer binds to the template DNA and extends, the 5'→3' exonuclease activity of the Taq enzyme will cut the TaqMan probe, so that the fluorescent reporter group and the quencher group are separated, thereby releasing a fluorescent signal. The intensity of the fluorescent signal is proportional to the amount of target nucleic acid produced in the reaction, and by detecting the change in the fluorescent signal, the progress and results of the LAMP-CRISPR reaction can be monitored in real time, and the amplification of the sample can also be observed under blue light to determine whether it emits fluorescence.

[0003] In the laboratory, the detection results can be detected using professional detection equipment (such as a fluorescence detector), but these professional equipment cannot be taken with them when they are outdoors, and these equipment cannot meet the rapid detection requirements of outdoor detection. CONTENT OF THE INVENTION

[0004] In view of the problems in the prior art, the present application provides a device for observing the detection results of outdoor portable LAMP-CRISPR, which comprises a container for containing LAMP-CRISPR samples to be detected, and a light source is arranged on the container for providing blue light for observing the LAMP-CRISPR samples.

[0005] On the basis of the above-mentioned scheme, the bottom wall of the container for placing the LAMP-CRISPR sample to be detected is a background wall for facilitating observation of the color of the sample.

[0006] On the basis of the above-mentioned scheme, the color of the background wall is black.

[0007] On the basis of the above-mentioned scheme, the light source is a blue LED or a blue flashlight.

[0008] On the basis of the above-mentioned scheme, when the light source is a blue flashlight, a light transmission hole for the blue flashlight to emit light into the LAMP-CRISPR sample is arranged on the side wall of the container.

[0009] The device is designed based on a fluorescent dye combined with naked eye observation method, is small in size, light in weight, and high in convenience, and can be used in various outdoor environments.

[0010] The device is simple to operate, does not need professionals and complex equipment, and reduces dependence on a laboratory environment.

[0011] The device can greatly reduce detection cost. BRIEF DESCRIPTION OF DRAWINGS

[0012] Figure 1 FIG. 1 is a structural schematic diagram of the device of the present application;

[0013] Figure 2 FIG. 2 is a structural schematic diagram (with a side wall unfolded) of the device of the present application;

[0014] Figure 3 FIG. 3 is a structural schematic diagram (with a light transmission hole) of the device of the present application. DETAILED DESCRIPTION

[0015] In order to further illustrate the technical means and effects adopted by the present application to achieve the predetermined purposes, the specific embodiments, structures, features and effects of the present application will be described in detail below in combination with the drawings and preferred embodiments.

[0016] As shown in FIGS. Figure 1 and Figure 2 The present application provides a specific embodiment of a device for observing LAMP-CRISPR detection results outdoors, which comprises a container 1 for containing a LAMP-CRISPR sample to be detected, a light source 2 is arranged on the container 1 to provide blue light for observing the LAMP-CRISPR sample, and a yellow light film 3 is further arranged on the wall of the container 1 to observe the LAMP-CRISPR sample.

[0017] In use, only need to put the sample to be observed detection in the container 1, open the light source 2, the observer can see from the yellow light film 3 whether the sample is positive or not. If there is no yellow light film 3, using only blue light source, the color of the positive sample is not very obvious (only some turbidity); under the blue light irradiation of the light source 2, the positive sample with obvious fluorescence can be observed through the yellow light film 3.

[0018] The container 1 actually plays a role of a dark box, therefore, the walls of the container 1 are all light-proof, that is, the material of the container 1 uses light-proof material, so that the influence of external natural light can be effectively reduced.

[0019] The purpose of using the yellow light film 3 in the device of the utility model is to filter part of the blue light, reduce the stimulation of the blue light to the eyes, and make the result of emitting fluorescence more easily distinguished by naked eyes. The yellow light film 3 is cheap and can be recycled.

[0020] The walls of the whole device except the yellow light film 3 use light-proof material, and the purpose of the setting is to effectively block the stray light in the surrounding environment from entering the device, prevent interference, and cause the observed experimental result to be not obvious.

[0021] As a preferred embodiment, in order to more clearly observe the LAMP-CRISPR sample, the bottom wall of the container 1 for placing the LAMP-CRISPR sample to be detected is a background wall 1-1 for facilitating the observation of the color of the sample. Further, the color of the background wall 1-1 is black.

[0022] In field detection, the portability of the device is very important, therefore, the light source 2 emitting blue light cannot use the traditional ultraviolet lamp, because the traditional ultraviolet lamp tube has a relatively large volume, and needs a relatively large voltage, and is not suitable for carrying in the wild. At the same time, the service life of the traditional ultraviolet lamp is short, for example, the average service life of the common ultraviolet lamp tube is only several thousand hours, frequent switching can shorten the service life, and needs to be replaced regularly. In addition, the ultraviolet lamp tube usually needs a certain preheating time to reach the best working state, which affects the experimental efficiency. At the same time, the ultraviolet lamp tube generates a lot of heat, and needs good heat dissipation design, otherwise the performance and service life of the instrument can be affected. Therefore, as a specific embodiment, the light source 2 is a blue light LED. The blue light LED can be directly arranged on the inner wall of the container 1, and the power supply uses a storage battery (primary battery or secondary battery).

[0023] As shown in Figure 3 The light source 2 can also use a blue light flashlight convenient to carry, and when the blue light flashlight is used, the side wall of the container 1 is provided with a light transmission hole 1-2 for the light source of the blue light flashlight to enter the LAMP-CRISPR sample.

[0024] The size of the light transmission hole 1-2 can only accommodate the light tube of a blue light flashlight, which is placed on the back light side during use, with the purpose of reducing the interference of other light sources on the result.

[0025] The above is only the preferred embodiment of the present application, and does not limit the present application in any form. Although the present application has been disclosed as above with the preferred embodiment, it is not intended to limit the present application. Any person skilled in the art can make some changes or modifications to the above disclosed technical content to obtain equivalent embodiments with equivalent changes, without departing from the technical solution of the present application. Any modification, equivalent change and modification of the above embodiments made according to the technical essence of the present application, without departing from the technical solution of the present application, still belongs to the scope of the technical solution of the present application.

Claims

1. A device for observing portable LAMP-CRISPR detection results outdoors, characterized in that, It includes a container (1) for holding LAMP-CRISPR samples to be tested, and a light source (2) for providing blue light for observing LAMP-CRISPR samples is provided on the container (1); a yellow light film (3) for observing LAMP-CRISPR samples is also provided on the wall of the container (1).

2. The device for outdoor portable LAMP-CRISPR detection result observation according to claim 1, characterized in that, The container (1) is used to place the LAMP-CRISPR sample to be tested. The bottom wall is a background wall (1-1) that facilitates observation of the sample color.

3. The device for outdoor portable LAMP-CRISPR detection result observation according to claim 2, characterized in that, The background wall (1-1) is black.

4. The device for outdoor portable LAMP-CRISPR detection result observation according to claim 2, characterized in that, The light source (2) is a blue LED or a blue flashlight.

5. The device for outdoor portable LAMP-CRISPR detection result observation according to claim 2, characterized in that, When the light source (2) is a blue light flashlight, the side wall of the container (1) is provided with a light-transmitting hole (1-2) for the light source of the blue light flashlight to enter the LAMP-CRISPR sample.

6. The device for outdoor portable LAMP-CRISPR detection result observation according to claim 1, characterized in that, The walls of the container (1) are all opaque.

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