Continuous flow biological reaction system for producing D-psicose

Abstract

The embodiment of the utility model discloses a continuous flow biological reaction system for producing D-psicose. The continuous flow biological reaction system comprises a raw material barrel, a reaction kettle, a transfer kettle, a decolorization device and a separation device which are connected in sequence, the reaction kettle comprises a kettle body, a top cover and an enzyme catalysis column; a reaction material inlet is formed in the lower part of the kettle body; a reaction material outlet is formed in the upper part of the kettle body; the top cover hermetically covers the top opening of the kettle body; a plurality of enzyme catalysis columns are arranged, are detachably connected with the top cover and extend into the kettle body; each enzyme catalysis column comprises an outer protective net and a filter membrane, the filter membrane is located in the outer protective net, and catalytic enzymes are embedded and fixed in pores of the filter membrane. The embodiment of the utility model is particularly suitable for continuous production of D-psicose through glucose isomerase and D-psicose-3-epimerase, repeated utilization of enzyme is ensured in the production process, the production efficiency of D-psicose is improved, and the method has an industrial application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of D-psicose production equipment, and particularly relates to a continuous flow bioreaction system for producing D-psicose. BACKGROUND

[0002] Rare sugars are a class of monosaccharides and their derivatives with extremely low content in nature, which have wide application prospects in the fields of food, medicine and the like. D-psicose is one of the representatives of rare sugars, which has 70% of the sweetness of sucrose and only 0.3% of the heat of sucrose, and has multiple physiological functions such as maintaining blood glucose balance, regulating lipid metabolism and anti-obesity, and is a potential functional sweetener.

[0003] D-psicose is difficult to be directly extracted from nature due to its extremely low content in nature, and thus is generally prepared by a synthetic route. The preparation method of D-psicose mainly includes chemical synthesis and biological synthesis. Due to the problems such as complex reaction process, high purification cost and difficult separation of byproducts in the chemical synthesis method, the biological synthesis method is more widely used, in which glucose is used as a substrate and is catalytically converted by glucose isomerase and D-psicose 3-epimerase.

[0004] However, in the production of D-psicose by using glucose isomerase and D-psicose 3-epimerase, there are problems of low enzyme catalytic efficiency and large raw material loss. Specifically, if free enzymes are used for catalysis, the free enzymes only participate in one catalytic reaction, the catalytic efficiency is low, and the enzymes are difficult to be recycled. If the fixed enzyme packed bed reactor is used for catalysis, the fixed enzyme is easy to be damaged due to extrusion, and the problem of low enzyme catalytic efficiency also exists. Moreover, the entire production system usually adopts a non-continuous production with multiple steps, which further leads to low production efficiency. CONTENT OF THE INVENTION

[0005] In order to solve at least one problem mentioned in the background, the present application provides a continuous flow bioreaction system for producing D-psicose, which is particularly suitable for continuous production of D-psicose by using glucose isomerase and D-psicose 3-epimerase, and ensures the reuse of enzymes in the production process, improves the production efficiency of D-psicose, and has the prospect of industrial application.

[0006] In order to achieve the above-mentioned purpose, the present application provides a continuous flow bioreaction system for producing D-psicose, which comprises a raw material barrel, a reaction kettle, a transfer kettle, a decolorizing device and a separation device.

[0007] The reaction kettle comprises a kettle body, a top cover and enzyme catalysis columns; the top of the kettle body is open, the lower part of the kettle body is provided with a reaction material inlet, and the upper part of the kettle body is provided with a reaction material outlet; the top cover is arranged on the top of the kettle body; the enzyme catalysis columns are arranged in plurality, each of the enzyme catalysis columns is detachably connected with the top cover and extends into the kettle body; each of the enzyme catalysis columns comprises an outer protective net and a filter membrane, the filter membrane is located inside the outer protective net, and a catalytic enzyme is embedded and fixed in the pores of the filter membrane;

[0008] The raw material barrel is provided with a raw material inlet, a return material inlet and a raw material outlet, the transfer kettle is provided with a transfer inlet and a transfer outlet, the decolorizing device is provided with a decolorizing inlet and a decolorizing outlet, and the separation device is provided with a separation inlet, a separation product outlet and a recycled material outlet;

[0009] The raw material outlet is communicated to the reaction material inlet through a raw material pipeline, and a control valve is arranged on the raw material pipeline; the reaction material outlet is communicated to the transfer inlet, the transfer outlet is communicated to the decolorizing inlet, the decolorizing outlet is communicated to the separation inlet, and the recycled material outlet is communicated to the return material inlet.

[0010] In an implementable embodiment, the filter membrane is provided with multiple layers, and the filter membrane comprises a porous membrane;

[0011] The multiple layers of the filter membrane are coaxially arranged or the multiple layers of the filter membrane are arranged from the center and synchronously wound outward, and the pores of each layer of the filter membrane are embedded and filled with the catalytic enzyme;

[0012] The catalytic enzyme comprises a first catalytic enzyme and a second catalytic enzyme, and different types of catalytic enzymes are embedded on adjacent layers of the filter membrane.

[0013] In an implementable embodiment, the outer protective net comprises a top connecting ring, an outer peripheral protective net and a bottom protective net, the top connecting ring is arranged around the top position of the outermost layer of the filter membrane, the outer peripheral protective net is connected below the top connecting ring and around the middle and lower positions of the outermost layer of the filter membrane, and the bottom protective net is connected below the outer protective net and arranged at the bottom of each of the filter membranes.

[0014] In an implementable embodiment, a blind hole is formed in the inside of the top cover of the reaction kettle, and the top connecting ring is detachably connected in the blind hole.

[0015] In an implementable embodiment, the enzyme catalysis column further comprises a sealing cover, the sealing cover is connected to the upper part of the top connecting ring, and the outer diameter of the sealing cover is greater than the outer diameter of the top connecting ring;

[0016] The top cover of the reactor is provided with a through hole; the top connecting ring is detachably connected to the through hole, and the sealing cover is arranged on the upper surface of the top cover, or the top connecting ring penetrates through the through hole, and the sealing cover is detachably connected to the top cover.

[0017] In an implementable embodiment, the kettle body is cylindrical, and the plurality of enzyme catalysis columns are arranged in a circumferential array on the top cover.

[0018] In an implementable embodiment, the reactor further comprises a support connected to the bottom of the kettle body, the reaction material inlet is arranged on the lower bottom surface of the kettle body, and the reaction material outlet is arranged on the upper side surface of the kettle body.

[0019] In an implementable embodiment, a metering pump and a flow meter are arranged on the raw material pipeline.

[0020] In an implementable embodiment, a heat exchanger is arranged on the raw material pipeline, the outer side wall of the reactor is provided with a heating jacket, and the interior of the reactor is provided with a temperature sensor.

[0021] In an implementable embodiment, the decolorizing device comprises an ion exchange resin column, and the separating device comprises a simulated moving bed.

[0022] The embodiment of the present application provides a continuous flow biological reaction system for producing D-psicose, a reaction kettle is arranged, including a kettle body, a top cover and enzyme catalysis columns, the top cover is sealed to the top opening of the kettle body, and the enzyme catalysis columns are detachably connected to the top cover, so that the enzyme catalysis columns can be replaced after a certain working time according to production needs, the enzyme catalysis efficiency is improved, and the raw material loss is reduced. The enzyme catalysis columns are arranged to include an outer protective net and a filter membrane, and the catalytic enzyme is embedded and fixed in the pores of the filter membrane, so that the movement of the catalytic enzyme is avoided, the catalytic enzyme is prevented from being damaged due to flow impact and extrusion, the enzyme catalysis efficiency can be improved, the enzyme can be reused, and the raw material loss is reduced. Multiple groups of enzyme catalysis columns are arranged, high-efficiency conversion can be realized, the purity of D-psicose is improved, and the yield of reactants is increased. A highly integrated system of a raw material barrel, a reaction kettle, a transfer kettle, a decolorizing device and a separating device is arranged, the separating device is provided with a reused material outlet, and the usable raw materials are added to the raw material barrel through a reused material inlet to participate in cyclic production, so that continuous production of D-psicose is realized, the raw materials are reused, the raw material waste is reduced, and the production cost is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings in the following description are some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor on the basis of these drawings.

[0024] Figure 1 A schematic diagram of a continuous flow bioreaction system for producing D-allulose provided by an embodiment of the present application;

[0025] Figure 2 A structural schematic diagram of a reaction kettle provided by an embodiment of the present application.

[0026] Legend of reference signs:

[0027] 100-continuous flow bioreaction system;

[0028] 110-raw material barrel; 111-raw material inlet; 112-returned material inlet; 113-raw material outlet;

[0029] 120-reaction kettle; 121-kettle body; 1211-reaction material inlet; 1212-reaction material outlet; 1213-heating jacket; 1214-temperature sensor; 122-top cover; 1221-sealing ring; 123-enzyme catalysis column; 1231-outer protective net; 1232-filtration membrane; 1233-sealing cover; 124-bracket;

[0030] 130-transit kettle; 131-transit feed inlet; 132-transit discharge outlet;

[0031] 140-decoloring device; 141-decoloring feed inlet; 142-decoloring discharge outlet;

[0032] 150-separation device; 151-separation feed inlet; 152-separation product outlet; 153-recycled material outlet;

[0033] 160-raw material pipeline; 161-control valve; 162-metering pump; 163-flow meter; 164-heat exchanger. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solutions and advantages of the present application clearer, the following will describe the technical solutions in the embodiments of the present application in more detail with reference to the drawings in the embodiments of the present application. It should be noted that the embodiments described in the drawings are only some embodiments of the present application, not all embodiments. That is, the embodiments described by the drawings are exemplary and are intended to explain the present application, and cannot be understood as a limitation of the present application.

[0035] The following will be described in combination withFigure 1 and Figure 2 The continuous flow bioreaction system 100 for producing D-psicose provided by the embodiments of the present application is described.

[0036] The embodiments of the present application provide a continuous flow bioreaction system 100 for producing D-psicose, which, as shown in Figure 1 and Figure 2 includes a raw material barrel 110, a reaction kettle 120, a transfer kettle 130, a decolorizing device 140, and a separation device 150.

[0037] The reaction kettle 120 includes a kettle body 121, a top cover 122, and a plurality of enzyme catalysis columns 123. The kettle body 121 is open at the top, and a reaction material inlet 1211 is arranged at the lower part of the kettle body 121, and a reaction material outlet 1212 is arranged at the upper part of the kettle body 121. The top cover 122 is arranged to seal the top opening of the kettle body 121. The plurality of enzyme catalysis columns 123 are arranged to be detachably connected to the top cover 122 and extend into the interior of the kettle body 121. Each enzyme catalysis column 123 includes an outer protective net 1231 and a filter membrane 1232, and the filter membrane 1232 is arranged inside the outer protective net 1231, and a catalytic enzyme is embedded and fixed in the pores of the filter membrane 1232.

[0038] The raw material barrel 110 is provided with a raw material inlet 111, a return material inlet 112, and a raw material outlet 113, the transfer kettle 130 is provided with a transfer material inlet 131 and a transfer material outlet 132, the decolorizing device 140 is provided with a decolorizing material inlet 141 and a decolorizing material outlet 142, and the separation device 150 is provided with a separation material inlet 151, a separation product outlet 152, and a recycled material outlet 153.

[0039] The raw material outlet 113 is connected to the reaction material inlet 1211 through a raw material pipeline 160, and a control valve 161 is arranged on the raw material pipeline 160. The reaction material outlet 1212 is connected to the transfer material inlet 131, the transfer material outlet 132 is connected to the decolorizing material inlet 141, the decolorizing material outlet 142 is connected to the separation material inlet 151, and the recycled material outlet 153 is connected to the return material inlet 112.

[0040] The raw material barrel 110 is used to contain a glucose solution raw material. The raw material inlet 111 and the return material inlet 112 are arranged on the top surface of the raw material barrel 110, and the raw material outlet 113 is arranged on the bottom surface of the raw material barrel 110.

[0041] According to the production requirements, the number of enzyme catalysis columns 123 can be 4-8, and 4 enzyme catalysis columns 123 are shown in the embodiment of the present application. The catalytic enzymes on the enzyme catalysis columns 123 include glucose isomerase and D- Psicose-3-epimerase. One enzyme catalysis column 123 can be filled with one catalytic enzyme, and another enzyme catalysis column 123 can be filled with another catalytic enzyme. Alternatively, two different catalytic enzymes can be filled in the same enzyme catalysis column 123 as described below. The catalytic enzymes are embedded and filled in the pores of the filter membrane 1232 to participate in the reaction in an immobilized form, preventing the catalytic enzymes from falling off due to the impact of liquid flow. The minimum diameter of the mesh of the protective mesh 1231 is smaller than the minimum outer size of the catalytic enzymes, which can limit the catalytic enzymes in the protective mesh 1231 and prevent the loss caused by the liquid flow, thereby improving the enzyme catalysis efficiency. The enzyme catalysis column 123 is detachably connected with the top cover 122, which facilitates the replacement of the enzyme catalysis column 123 and enables the updated enzyme catalysis column 123 to achieve better enzyme catalysis conversion effect.

[0042] In the reaction kettle 120, the glucose in the glucose solution at the reaction material inlet 1211 is catalyzed by glucose isomerase to generate fructose, and the fructose is catalyzed by D- Psicose-3-epimerase to obtain D- Psicose. The reaction mixture solution containing D- Psicose, un-catalyzed fructose and un-reacted glucose after the reaction flows out from the reaction material outlet 1212. The reaction material inlet 1211 can be arranged on the side surface of the lower part of the kettle body 121, or can be arranged on the bottom surface of the lower part of the kettle body 121 as described below. The reaction material outlet 1212 can be arranged on the side surface of the upper part of the kettle body 121. The top cover 122 of the reaction kettle 120 can be threadedly connected with the kettle body 121, or can be connected by clamping.

[0043] The transfer kettle 130 is used for storing the reaction mixture solution flowing out from the reaction kettle 120. The transfer inlet 131 and the transfer outlet 132 of the transfer kettle 130 can be arranged opposite to each other in the up-down direction.

[0044] The decolorizing device 140 is used for decolorizing the reaction mixture solution flowing out from the transfer kettle 130. The decolorizing outlet 142 and the decolorizing inlet 141 of the decolorizing device 140 can be arranged opposite to each other in the left-right direction. In some embodiments, the decolorizing inlet 141 and the decolorizing outlet 142 can also be arranged opposite to each other in the up-down direction.

[0045] The separation device 150 is used for separating the reaction mixture solution after decolorization to obtain the product D- Psicose solution and the mixed solution of fructose and glucose. The D- Psicose solution flows out from the separation product outlet 152 and is concentrated and crystallized to obtain D- Psicose. The mixed solution of fructose and glucose flows out from the reuse material outlet 153 and flows into the raw material barrel 110 again through the raw material inlet 112 for recycling. The separation product outlet 152 of the separation device 150 can be arranged below. The separation inlet 151 and the reuse material outlet 153 can be arranged opposite to each other in the left-right direction.

[0046] The control valve 161 can be a gate valve, which facilitates the control of the on / off connection between the raw material tank 110 and the reactor 120.

[0047] This application provides a continuous flow bioreactor system for producing D-allulose. The reactor includes a vessel body, a top cover, and an enzyme catalytic column. The top cover is sealed, but the top of the vessel body is open. The enzyme catalytic column is detachably connected to the top cover, facilitating replacement after a certain operating time as needed, thus improving enzyme catalytic efficiency and reducing raw material loss. The enzyme catalytic column includes an outer protective mesh and a filter membrane. The catalytic enzyme is embedded and fixed in the pores of the filter membrane, preventing enzyme movement and protection from flow impact and damage, further improving enzyme catalytic efficiency and reducing raw material loss. Multiple enzyme catalytic columns enable efficient conversion, increasing the purity of D-allulose and the yield of reactants. The system is highly integrated with a raw material tank, reactor, transfer tank, decolorization device, and separation device. The separation device has a recycling outlet, allowing usable raw materials to be added back to the raw material tank through the recycling inlet for continuous production of D-allulose. This achieves continuous production of D-allulose, reuses raw materials, reduces waste, and lowers production costs.

[0048] In one feasible implementation, refer to Figure 2 As shown, the filter membrane 1232 is provided with multiple layers, and the filter membrane 1232 includes a porous membrane. The multiple filter membranes 1232 are coaxially arranged or the multiple filter membranes 1232 are arranged side by side from the center and wound outward synchronously. The pores of each filter membrane 1232 are embedded and filled with a catalytic enzyme. The catalytic enzymes include a first catalytic enzyme and a second catalytic enzyme, and different types of catalytic enzymes are embedded on adjacent filter membrane layers 1232.

[0049] The first and second catalytic enzymes can correspond to glucose isomerase and D-allulose-3-epimerase, respectively. The enzymes can be immobilized using porous membranes with polar groups, as is currently available, allowing the catalytic enzymes to be embedded in the pores of the membrane. The filter membrane 1232 can have 4-6 layers, with different types of catalytic enzymes on adjacent layers. For example, the outermost porous membrane layer may contain immobilized glucose isomerase particles, the second porous membrane layer may contain immobilized D-allulose-3-epimerase particles, and so on for layers 3-6. In one embodiment, the immobilized D-allulose-3-epimerase is spherical with a diameter of 340 μm, the pore size of the porous membrane ranges from 340 μm to 360 μm, and the pore size of the outer protective mesh ranges from 325 μm to 340 μm.

[0050] In this way, two different catalytic enzymes can be distributed on the same enzyme catalytic column 123, which facilitates the catalytic transformation of the raw material solution by the action of the catalytic enzymes.

[0051] In one feasible implementation, the outer protective net 1231 includes a top connecting ring, an outer peripheral protective net, and a bottom protective net. The top connecting ring surrounds the top position of the outermost filter membrane 1232, the outer peripheral protective net is connected below the top connecting ring and surrounds the middle and lower positions of the outermost filter membrane 1232, and the bottom protective net is connected below the outer protective net 1231 and disposed at the bottom of each filter membrane 1232.

[0052] The outer protective net 1231 can be a stainless steel metal part, and the porous membrane with the immobilized catalytic enzyme particles is installed inside the outer protective net 1231.

[0053] In this way, the outer protective net 1231 not only confines the catalytic enzyme particles to prevent the catalytic enzyme from being lost and to ensure the enzyme catalytic efficiency, but also serves to connect the top cover 122.

[0054] In one feasible implementation, a blind hole is provided on the inner side of the top cover 122 of the reactor 120, and a top connecting ring is detachably connected to the blind hole.

[0055] The top connecting ring may have a certain thickness and be machined with external threads. The blind hole of the top cover 122 is machined with internal threads, and the top connecting ring is connected to the top cover 122 by the threads.

[0056] In this way, after the set production time, the enzyme catalytic column 123 can be disassembled and replaced by opening the top cover 122, and the catalytic efficiency can be improved by using a new catalytic enzyme.

[0057] In one feasible implementation, refer to Figure 2 As shown, the enzyme catalytic column 123 also includes a sealing cap 1233, which is connected to the upper part of the top connecting ring, and the outer diameter of the sealing cap 1233 is larger than the outer diameter of the top connecting ring.

[0058] The top cover 122 of the reactor 120 has a through hole. A top connecting ring is detachably connected to the through hole, and a sealing cover 1233 is placed on the upper surface of the top cover 122. Alternatively, the top connecting ring passes through the through hole, and the sealing cover 1233 is detachably connected to the top cover 122.

[0059] Preferably, the sealing cap 1233 and the top connecting ring are detachably connected by means of snap-fit, threaded connection, magnetic connection, etc., which makes it easy to remove the outer protective net 1231 and the sealing cap 1233, and then replace the filter membrane 1232 and the catalytic enzyme.

[0060] In some embodiments, the top connecting ring and the through hole can be threaded together, and the sealing cover 1233 covers the top cover 122 through the sealing ring 1221 to prevent the solution in the reactor 120 from overflowing.

[0061] In other embodiments, the top connecting ring may simply pass through the through hole, with a small gap between them. A raised ring may be provided around the through hole on the upper surface of the top cover 122, with external threads machined on the raised ring, and an internal thread machined on the sealing cover 1233, which is threadedly connected to the top cover 122. Alternatively, multiple snap-fit ​​protrusions may be provided around the through hole on the upper surface of the top cover 122, and a snap-fit ​​may be machined on the sealing cover 1233, which is snap-fitted to the top cover 122. Or, the sealing cover 1233 may be magnetically connected to the top cover 122.

[0062] In this way, after the set production time, the enzyme catalytic column 123 can be directly disassembled, extracted, and replaced without opening the top cover 122, so as to use a new catalytic enzyme to improve catalytic efficiency.

[0063] In one feasible implementation, refer to Figure 1 As shown, the vessel body 121 is cylindrical, and multiple enzyme catalytic columns 123 are arranged in a circumferential array on the top cover 122.

[0064] This allows for a more uniform distribution of the catalytic enzyme, promoting increased reaction yield and improving enzyme catalytic efficiency.

[0065] In one feasible implementation, refer to Figure 1 and Figure 2 As shown, the reactor 120 also includes a support 124, which is connected to the bottom of the reactor body 121. The reaction material inlet 1211 is located on the lower bottom surface of the reactor body 121, and the reaction material outlet 1212 is located on the upper side surface of the reactor body 121.

[0066] In this way, the reactor 120 can be supported by the bracket 124, which makes it easy to set the reaction material inlet 1211 on the bottom surface of the reactor body 121.

[0067] In one feasible implementation, refer to Figure 1 As shown, a metering pump 162 and a flow meter 163 are installed on the raw material pipeline 160. Specifically, a metering pump 162 and a flow meter 163 are sequentially installed on the raw material pipeline 160 from the control valve 161 to the reactor 120.

[0068] Among them, the metering pump 162 is a diaphragm pump and the flow meter 163 is an electromagnetic flow meter 163. The metering pump 162 and the flow meter 163 are used to control the flow rate of the raw materials into the reaction vessel 120, so that the raw materials can react in the enzyme catalytic column 123 and the reaction process can be controlled at a better flow rate.

[0069] The prepared glucose solution with a final concentration of 50% enters the raw material tank 110, and the glucose solution in the raw material tank 110 is slowly flowed into the reactor 120 at a flow rate of 0.4-4 mL / min by using the metering pump 162 and the flow meter 163. In the embodiment of the application, the glucose solution is slowly flowed into the reactor 120 at a flow rate of 3 mL / min. The reaction mixture obtained after the reaction is flowed out from the reaction material outlet 1212 to the transfer reactor 130, and is collected after separation, thereby reducing the waste of raw materials.

[0070] In an implementable embodiment, referring to FIGS. 1, 2 and 3, the raw material pipeline 160 is provided with a heat exchanger 164, the outer wall of the reactor 120 is provided with a heating jacket 1213, and the inside of the reactor 120 is provided with a temperature sensor 1214. Figure 1 and Figure 2 Specifically, the heat exchanger 164 is arranged on the raw material pipeline 160 between the flow meter 163 and the reactor 120, water or oil is used as the heating medium in the heating jacket 1213, and the temperature sensor 1214 is installed on the top cover 122 with its probe extending into the reactor body 121.

[0071] The heat exchanger 164 is a plate heat exchanger 164, which can preheat the glucose solution flowed from the raw material tank 110 to an optimal reaction temperature, preferably to 30-55°C.

[0072] Meanwhile, the heat preservation jacket can ensure that the immobilized enzyme and the raw material maintain an optimal reaction environment during the reaction. The heat preservation jacket ensures that the enzyme catalytic column 123 in the reactor 120 reacts at 30-55°C.

[0073] The temperature sensor 1214 arranged in the reactor 120 is used to detect the temperature of the raw material flowed from the heat exchanger 164, which is used as a reference to further control and adjust the heating temperature of the heat preservation jacket and the heat exchanger 164.

[0074] In an implementable embodiment, the decolorizing device 140 comprises an ion exchange resin column, and the separating device 150 comprises a simulated moving bed.

[0075] The reaction mixture in the transfer reactor 130 is subjected to decolorization by the ion exchange resin column, and the decolored reaction mixture is subjected to chromatographic separation operation by the simulated moving bed to obtain a product D-psicose solution and a fructose and glucose mixed solution. The separated fructose and glucose mixed solution is returned to the raw material tank 110 for further reaction, and the obtained D-psicose solution is subjected to concentration and crystallization operation to obtain D-psicose.

[0076] It should be noted that, unless otherwise explicitly specified and limited, in the description of the present application, the terms "mounting", "connection", "linking" should be interpreted in a broad sense, for example, it can be fixed connection, or indirect connection through intermediate medium, or internal communication of two elements, or interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0077] The terms "upper", "lower", "front", "back", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0078] The term "a plurality of" means two or more, unless otherwise explicitly specified and limited.

[0079] The terms "first", "second", "third", "fourth" and the like (if any) are used to distinguish similar objects, and do not necessarily have to describe a particular order or sequence. It should be understood that the data thus used can be interchanged under appropriate circumstances, so that the embodiments of the present application described herein can include orders other than those illustrated or described herein.

[0080] The terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device including a series of steps or units does not have to be limited to those steps or units clearly listed, but can include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0081] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A continuous flow bioreaction system for producing D-allulose, characterized by, The device comprises a raw material barrel, a reaction kettle, a transfer kettle, a decolorizing device and a separation device. The reaction kettle comprises a kettle body, a top cover and enzyme catalysis columns; the top of the kettle body is open; a reaction material inlet is arranged at the lower part of the kettle body; a reaction material outlet is arranged at the upper part of the kettle body; the top cover is arranged to seal the open top of the kettle body; the enzyme catalysis columns are arranged in plurality; each of the enzyme catalysis columns is detachably connected to the top cover and extends into the kettle body; each of the enzyme catalysis columns comprises an outer protective net and a filter membrane; the filter membrane is arranged inside the outer protective net; a catalytic enzyme is embedded and fixed in the pores of the filter membrane. The raw material barrel is provided with a raw material inlet, a return material inlet and a raw material outlet; the transfer kettle is provided with a transfer inlet and a transfer outlet; the decolorizing device is provided with a decolorizing inlet and a decolorizing outlet; the separation device is provided with a separation inlet, a separation product outlet and a recycled material outlet. The raw material outlet is connected to the reaction material inlet through a raw material pipeline; the reaction material outlet is connected to the transfer inlet; the transfer outlet is connected to the decolorizing inlet; the decolorizing outlet is connected to the separation inlet; the recycled material outlet is connected to the return material inlet.

2. The continuous flow bioreactor system for D-psicose production according to claim 1, wherein, The filter membrane is provided with multiple layers; the filter membrane comprises a porous membrane. The multiple layers of the filter membrane are coaxially arranged or are synchronously wound from the center; the pores of each layer of the filter membrane are embedded and filled with the catalytic enzyme. The catalytic enzyme comprises a first catalytic enzyme and a second catalytic enzyme; different types of catalytic enzymes are embedded on adjacent layers of the filter membrane.

3. The continuous flow bioreactor system for D-psicose production according to claim 2, wherein, The outer protective net comprises a top connecting ring, an outer peripheral protective net and a bottom protective net; the top connecting ring is arranged around the top of the outermost layer of the filter membrane; the outer peripheral protective net is connected below the top connecting ring and is arranged around the middle and lower parts of the outermost layer of the filter membrane; the bottom protective net is connected below the outer protective net and is arranged at the bottom of each of the filter membranes.

4. The continuous flow bioreactor system for D-psicose production according to claim 3, wherein, A blind hole is arranged in the inside of the top cover of the reaction kettle; the top connecting ring is detachably connected in the blind hole.

5. The continuous flow bioreactor system for D-psicose production according to claim 3, wherein, The enzyme catalysis column further comprises a sealing cover; the sealing cover is connected to the upper part of the top connecting ring; the outer diameter of the sealing cover is larger than that of the top connecting ring. A through hole is arranged in the top cover of the reaction kettle; the top connecting ring is detachably connected to the through hole; the sealing cover is arranged on the upper surface of the top cover; alternatively, the top connecting ring penetrates through the through hole; the sealing cover is detachably connected to the top cover.

6. The continuous flow bioreactor system for D-psicose production according to any one of claims 1 to 5, wherein, The kettle body is cylindrical; the enzyme catalysis columns are arranged in a circumferential array on the top cover.

7. The continuous flow bioreactor system for D-psicose production according to any one of claims 1 to 5, wherein The reaction kettle further comprises a support; the support is connected to the bottom of the kettle body; the reaction material inlet is arranged on the lower bottom surface of the kettle body; the reaction material outlet is arranged on the upper side surface of the kettle body.

8. The continuous flow bioreactor system for producing D-psicose according to any one of claims 1 to 5, wherein A metering pump and a flow meter are arranged on the raw material pipeline.

9. The continuous flow bioreactor system for D-psicose production according to any one of claims 1 to 5, wherein, A heat exchanger is arranged on the raw material pipeline; a heating jacket is arranged on the outer sidewall of the reaction kettle; a temperature sensor is arranged in the inside of the reaction kettle.

10. The continuous flow bioreactor system for producing D-psicose according to any one of claims 1 to 5, wherein The decolorizing device comprises an ion exchange resin column; the separation device comprises a simulated moving bed.

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