Buffer solution conveying device for protein separation

Abstract

The buffer solution conveying device comprises a buffer solution box, a sealing cover is arranged at the upper end of the buffer solution box, a plurality of locking devices are arranged on the peripheries of the bottoms of the two sides of the sealing cover, a pressurizing opening is formed in one side of the upper end of the sealing cover, and a liquid inlet is formed in the end, opposite to the pressurizing opening, of the upper end of the sealing cover. A handle is arranged in the center of the upper end of the sealing cover, adjusting supporting legs are arranged at the four corners of the bottom of the buffer solution box, a plurality of liquid drainage pipes are arranged at the bottom of the buffer solution box at equal intervals, and hoses are fixedly connected to the liquid drainage pipes; the product has the advantages that due to the design of the pressurizing opening and the external pressurizing device, the pressure in the buffer solution box is allowed to be increased through external pressure, so that the conveying efficiency of the buffer solution is improved, the flow speed of the buffer solution is more stable and controllable by controlling the external pressure, and liquid conveying in the protein separation process is optimized.

Description

TECHNICAL FIELD

[0001] The utility model relates to protein separation technical field especially relates to a buffer solution conveying device for protein separation. BACKGROUND

[0002] Protein separation is a common and crucial technical step in the fields of biochemical research, drug development, clinical diagnosis, etc. Traditional protein separation methods, such as gel electrophoresis, ion exchange chromatography, and affinity chromatography, rely on buffer solutions to maintain appropriate pH values and ionic strengths, ensuring protein stability and separation efficiency. In these separation processes, the delivery and control of buffer solutions are crucial, as they directly affect the precision, efficiency, and repeatability of the separation. Currently, buffer solutions used in protein separation processes are typically prepared manually by laboratory personnel and delivered to separation equipment (such as chromatography columns, centrifuge tubes, etc.) through pumping or gravity flow. SUMMARY

[0003] The purpose of the utility model is to overcome the problems of waste or incomplete use of buffer solution, increased experimental cost, the need for continuous monitoring of the solution state during manual operation, and the risk of human error due to the inaccuracy of flow rate and concentration control in traditional delivery methods.

[0004] The utility model discloses a buffer solution conveying device for protein separation, which includes a buffer solution box, a sealing cover is arranged at the upper end of the buffer solution box, a plurality of locking devices are arranged around the bottom of both sides of the sealing cover, a pressurizing port is arranged at one side of the upper end of the sealing cover, a liquid inlet is arranged at the opposite end of the pressurizing port, a handle is arranged at the center position of the upper end of the sealing cover, adjusting support legs are arranged at the bottom corners of the buffer solution box, a plurality of liquid discharge pipes are arranged at the bottom of the buffer solution box in equal intervals, a soft tube is fixedly connected to each liquid discharge pipe, the locking devices arranged on the sealing cover can effectively seal the buffer solution box, preventing solution leakage and ensuring safety during use, avoiding contamination or solution waste, the pressurizing port can increase the pressure in the buffer solution box through external pressure, thereby improving the delivery efficiency of the buffer solution, making the flow rate more stable and controllable during protein separation, optimizing the experimental process, the adjusting support legs arranged at the bottom corners of the buffer solution box can keep the device stable on different ground surfaces, avoiding solution tilting or overflow due to unevenness, providing flexible height adjustment, allowing adjustment of other devices at any time, the equally arranged liquid discharge pipes can uniformly deliver the buffer solution to multiple soft tubes, achieving liquid distribution and precise control, and being suitable for multi-point separation requirements.

[0005] Further, the buffer solution box is internally provided with a plurality of leak holes arranged equidistantly at the bottom end, a sealing ring is arranged on the bottom outer side of the sealing cover, the sealing ring is connected with the buffer solution box in a sealed manner, the plurality of leak holes arranged equidistantly at the bottom end of the buffer solution box can uniformly release the buffer solution at the bottom, the liquid is more uniformly distributed at the bottom of the solution box, the outflow speed of the buffer solution is better controlled, the solution is prevented from being accumulated or unevenly outflowed, the stability and precision of solution delivery are enhanced, the sealing ring arranged on the bottom outer side of the sealing cover can effectively prevent liquid leakage, the liquid in the device is prevented from leaking out through the sealed connection between the buffer solution box and the sealing cover, and the sealing design plays a crucial role in maintaining the stability of the liquid during the experiment and preventing pollution.

[0006] Further, the locking device comprises a fixing member, a bolt fixing nose is movably connected to the fixing member, and a buckle is arranged at the lower end of the fixing member.

[0007] Further, the fixing member is fixedly connected with the sealing cover, the buckle is fixedly connected with the buffer solution box, and the sealing cover is locked and sealed with the buffer solution box through the bolt fixing nose.

[0008] Further, the booster port is externally connected with a booster device for pressure injection in the cavity.

[0009] Beneficial effects: the utility model discloses the following beneficial effects:

[0010] 1. The design of the booster port and the externally connected booster device allows the pressure in the buffer solution box to be increased through external pressure, thereby improving the delivery efficiency of the buffer solution, and by controlling the external pressure, the flow rate of the buffer solution is more stable and controllable, and the liquid transmission in the protein separation process is optimized. This design helps to improve the experimental precision and ensures that the buffer solution is uniformly and effectively delivered to the required position.

[0011] 2. The sealing cover and the buffer solution box are connected in a sealed manner through the sealing ring and the locking device, so that the liquid cannot leak out. This sealing design prevents solution waste and pollution, ensures the safety during the experiment, and at the same time, the fixing member, the buckle and the bolt fixing nose jointly act to strengthen the firmness and durability of the buffer solution box and the sealing cover, making the device more reliable and avoiding accidental leakage during operation.

[0012] 3. The adjustable support legs at the four corners of the bottom of the buffer solution box are designed to enable the device to remain stable on different ground surfaces, avoiding solution tilting or overflow due to uneven ground. This design also provides flexible height adjustment function, enabling the device to adapt to different experimental environments or cooperate with other equipment, increasing the flexibility and adaptability of use, ensuring the stability during the experiment and the precise distribution of the solution. BRIEF DESCRIPTION OF DRAWINGS

[0013] Fig. 1 is a schematic view of the present application;

[0014] Fig. 2 is a cross-sectional schematic view of the present application;

[0015] Fig. 3 is an enlarged schematic view of the locking device of the present application;

[0016] In the figure: 1-buffer solution box, 2-sealing cover, 3-locking device, 4-pressurizing port, 5-liquid inlet, 6-handle, 7-adjustable support leg, 8-drainage pipe, 9-hose, 11-leakage hole, 21-sealing ring, 31-fixing member, 32-screw fixing nose, 33-buckle. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments.

[0018] In combination with Figs. 1 to 3As shown, a buffer solution delivery device for protein separation includes a buffer solution box 1, the upper end of the buffer solution box 1 is provided with a sealing cover 2, the bottom of both sides of the sealing cover 2 is provided with a plurality of locking devices 3, one side of the upper end of the sealing cover 2 is provided with a booster port 4, the upper end of the sealing cover 2 is provided with a liquid inlet 5 opposite the booster port 4, the center of the upper end of the sealing cover 2 is provided with a handle 6, the bottom of the buffer solution box 1 is provided with adjusting support legs 7 at four corners, a plurality of drainage pipes 8 are arranged at equal intervals at the bottom of the buffer solution box 1, a hose 9 is fixedly connected to each of the drainage pipes 8, the locking device provided on the sealing cover can effectively seal the buffer solution box, prevent solution leakage, ensure safety during use, avoid pollution or solution waste, the setting of the booster port can increase the pressure in the buffer solution box through external pressure, thereby improving the delivery efficiency of the buffer solution, making the flow rate more stable and controllable during protein separation, optimizing the experimental process, the adjusting support legs provided at the four corners of the bottom of the buffer solution box can keep the device stable on different ground, avoid solution tilting or overflow caused by unevenness, and provide flexible height adjustment, which can be adjusted at any time to adapt to other devices, the equally arranged drainage pipes can uniformly deliver the buffer solution to multiple hoses, realize liquid distribution and precise control, and are suitable for multi-point separation requirements.

[0019] Among them, a plurality of leak holes 11 are arranged at equal intervals at the bottom end of the buffer solution box 1, a sealing ring 21 is arranged on the outer side of the bottom of the sealing cover 2, and the sealing ring 21 is sealingly connected with the buffer solution box 1, the plurality of leak holes arranged at equal intervals at the bottom end of the buffer solution box can uniformly release the buffer solution at the bottom, ensure that the liquid is more uniformly distributed at the bottom of the solution box, and help better control the outflow speed of the buffer solution, avoid solution backlog or uneven outflow, and enhance the stability and precision of solution delivery, the sealing ring arranged on the outer side of the bottom of the sealing cover can effectively prevent liquid leakage, and the sealing connection between the sealing cover and the buffer solution box ensures that the liquid inside the device does not leak out, and this sealing design plays a crucial role in maintaining the stability of the liquid during the experiment and avoiding pollution; the locking device 3 includes a fixed part 31, a bolt fixing nose 32 movably connected to the fixed part 31, and a buckle 33 arranged at the lower end of the fixed part 31; the fixed part 31 is fixedly connected with the sealing cover 2, the buckle 33 is fixedly connected with the buffer solution box 1, the sealing cover 2 and the buffer solution box 1 are locked and sealed by the bolt fixing nose 32, and the fixed part, the buckle and the bolt fixing nose jointly improve the sealing performance, firmness and durability of the buffer solution box and the sealing cover; the booster port 4 is connected with a pressure boosting device for pressure injection in the cavity.

[0020] Working principle: the buffer solution box 1 is used to contain the buffer solution required in the separation process, the top of the box is closed by the sealing cover 2, the sealing cover is designed with various connection and locking devices to ensure the sealing and firmness of the device, prevent the solution from leaking, the locking device 3 on both sides of the sealing cover 2 includes a fixing piece 31, a bolt fixing nose 32 and a buckle 33, the fixing piece 31 is fixedly connected with the sealing cover 2, the buckle 33 is connected with the buffer solution box 1, and the bolt fixing nose 32 ensures that the sealing cover 2 and the buffer solution box 1 form a firm sealing through the locking device, which can prevent the buffer solution from leaking due to external factors such as pressure or vibration during use, and ensure the safety of the experimental process, the booster port 4 at the upper end of the sealing cover 2 is connected with an external booster device, the booster device provides a stable thrust by injecting external pressure into the buffer solution box, thereby improving the flow efficiency of the buffer solution, the booster device can adjust the pressure to ensure that the flow rate of the buffer solution is stable and controllable during the separation process, avoid flow rate fluctuation or uneven flow, thereby optimizing the solution delivery in the protein separation process, the equidistantly arranged drainage pipes 8 at the bottom of the buffer solution box 1 uniformly deliver the buffer solution to the multiple hoses 9, realizing precise liquid distribution, the hoses 9 can be connected to different separation equipment according to experimental needs, realizing multi-point liquid distribution, which is suitable for multi-point separation requirements in different experiments, by controlling the number, position and size of the drainage pipes, the solution delivery is further optimized, so that the solution flow rate is consistent with the experimental requirements.

[0021] It is obvious to those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting, and the scope of the present application is defined by the appended claims rather than the above description, and therefore all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present application. Any reference signs in the claims should not be regarded as limiting the claims involved.

[0022] In addition, it should be understood that although the present specification is described in terms of embodiments, not every embodiment contains only one independent technical solution, and the description of the specification is only for the sake of clarity, and those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can be combined appropriately to form other embodiments that those skilled in the art can understand.

Claims

1. A buffer solution delivery device for protein separation, comprising a buffer solution cartridge (1), characterized in that: The buffer solution box (1) is provided with a sealing cover (2) at the top. Several locking devices (3) are provided around the bottom of both sides of the sealing cover (2). A pressure port (4) is provided on one side of the top of the sealing cover (2). An inlet (5) is provided at the top of the sealing cover (2) opposite to the pressure port (4). A handle (6) is provided at the center of the top of the sealing cover (2). Adjustable support legs (7) are provided at the four corners of the bottom of the buffer solution box (1). Several drain pipes (8) are arranged at equal intervals at the bottom of the buffer solution box (1). A hose (9) is fixedly connected to each drain pipe (8).

2. The buffer solution delivery device for protein separation according to claim 1, characterized in that: The buffer solution box (1) has several leak holes (11) arranged at equal intervals at the bottom inside. The sealing cover (2) has a sealing ring (21) around its bottom outer side, and the sealing ring (21) is used to seal the buffer solution box (1).

3. A buffer solution delivery device for protein separation according to claim 2, characterized in that: The locking device (3) includes a fixing member (31), on which a bolt fixing lug (32) is movably connected, and a buckle (33) is provided at the lower end of the fixing member (31).

4. A buffer solution delivery device for protein separation according to claim 3, characterized in that: The fastener (31) is fixedly connected to the sealing cover (2), the buckle (33) is fixedly connected to the buffer solution box (1), and the sealing cover (2) and the buffer solution box (1) are locked and sealed by bolt fixing nose (32).

5. A buffer solution delivery device for protein separation according to claim 4, characterized in that: The pressure inlet (4) is connected to a pressure boosting device for injecting pressure into the cavity.

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