Long-time-sequence microorganism enrichment device

Abstract

The utility model discloses a long-time sequence microorganism enrichment device which comprises a sampling cup group, a planetary gear transmission assembly, a telescopic butt joint assembly and a water pumping assembly, the sampling cup group is composed of a plurality of independent sampling cup units, the sampling cup units are arranged on the index plate at equal intervals in a staggered manner, and each sampling cup unit is provided with a cup cover which can be opened independently; a plurality of docking ports are formed in the inner side and the outer side of the index plate at fixed intervals, and each docking port is communicated with each sampling cup unit; the dividing plate can rotate around the circle center under the driving of the planetary gear transmission assembly; the telescopic butt-joint assembly drives the sliding block to move, the sliding block is provided with a support, and the two ends of the support are provided with self-centering units which can be in butt joint with butt-joint openings in the index plate; each water pumping assembly comprises a water pump motor and a flow meter arranged on a water pumping pipeline of the water pump motor, and the non-butt-joint sides of the two self-centering units are connected with the water pumping pipeline of the corresponding water pumping assembly respectively. According to the device, a plurality of independent sampling cup units are arranged, so that a larger batch of samples can be treated at the same time, and the sample treatment capability is improved; meanwhile, the units are arranged at equal intervals in a staggered manner, so that impurities can be more efficiently removed, the filtering precision is improved, and the sample purity is improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of bioengineering technology, specifically a long time sequence microorganism enrichment device. BACKGROUND

[0002] With the continuous progress of biotechnology and environmental science, the demand for microorganism enrichment devices in scientific research, environmental monitoring, marine research and industrial applications is increasing. Microorganism enrichment technology is widely used in environmental management, wastewater treatment, food safety detection and biopharmaceuticals, and has great prospects in marine ecology, environmental monitoring and industrial bioreactors.

[0003] Currently, the microorganism enrichment devices on the market are mainly based on traditional filtration, concentration and separation methods. Most existing devices use simplified physical filtration and concentration principles when processing microbial samples. Common techniques include membrane filtration, centrifugal sedimentation, adsorption and other methods.

[0004] However, the existing microorganism enrichment devices also have some non-negligible shortcomings:

[0005] 1. Limited processing capacity: Many existing devices are only suitable for processing small batches of samples and cannot handle large-scale samples, which cannot meet the high-throughput and high-efficiency microorganism enrichment application requirements.

[0006] 2. Maintenance and service life issues: Most current devices have simple structures and lack modular design, making them prone to damage and difficult to maintain, especially in harsh environments such as deep sea exploration. The reliability of the device is poor, and it cannot guarantee long-term stable operation.

[0007] 3. Lack of flexibility and precision: Existing devices mostly fail to effectively address the precision issues of liquid injection, filtration and sample extraction during the microorganism enrichment process. Due to insufficient operational precision, sample processing may be uneven, resulting in unstable enrichment results and affecting the accuracy of experimental results.

[0008] In summary, although the existing technology can meet the basic needs of microorganism enrichment, there are still significant shortcomings in handling large-scale samples, improving enrichment efficiency and optimizing device performance. UTILITY MODEL CONTENT

[0009] The main purpose of the utility model is to provide a long time sequence microorganism enrichment device with high operational precision and easy replacement, as well as its application method.

[0010] The long-time sequence microorganism enrichment device comprises a sampling cup group, a planetary gear transmission assembly, a telescopic butt joint assembly and a water pumping assembly; the sampling cup group is composed of a plurality of independent sampling cup units, each sampling cup unit is arranged on a protractor disc at equal intervals, and the sampling cup unit is provided with a cup cover which can be opened independently; a plurality of butt joints are arranged on the inner side and the outer side of the protractor disc at fixed intervals, and each butt joint is connected with each sampling cup unit; the protractor disc can rotate along the center under the driving of the planetary gear transmission assembly; the telescopic butt joint assembly drives the displacement of the sliding block, a support is arranged on the sliding block, self-centering units are arranged at the two ends of the support, and the self-centering units can be butt jointed with the butt joints on the protractor disc; the water pumping assembly comprises a water pump motor and a flowmeter arranged on the water pumping pipeline of the water pump motor, and the two self-centering units are connected with the water pumping pipelines of the water pumping assembly on the non-butt joint side.

[0011] In an embodiment of the above device, two pipes of different sizes are arranged on the sampling cup unit and are connected with the 24 joint and the 10 joint on the top surface of the protractor disc respectively.

[0012] In an embodiment of the above device, the planetary gear transmission assembly comprises a base, a tray, a rotating disc support ring, a planetary gear pair and a protractor disc; the base is a circular thick seat, the tray is a same disc, and the tray is supported above the base through a plurality of support rods arranged at equal intervals along the circumference; the rotating disc support ring is arranged on the tray, the upper and lower end faces of the rotating disc support ring can rotate relative to the center, and the lower end face of the rotating disc support ring is fixed to the tray; the planetary gear pair comprises an outer ring gear ring and a cylindrical gear which are meshed with each other, the outer ring gear ring is fixed to the upper end face of the rotating disc support ring, the bottom end of the cylindrical gear is connected with the output shaft of a rotating motor, and the rotating motor is fixed to the base; and the protractor disc is fixed to the outer ring gear ring.

[0013] In an embodiment of the above device, a plurality of butt joints and plugs arranged at equal intervals along the circumference are arranged on the outer side of the protractor disc, a plurality of butt joints arranged at equal intervals along the circumference are arranged on the inner side of the protractor disc, the heights of the two groups of butt joints are consistent and the two groups of butt joints are located above the plugs; and the butt joints on the outer side and the inner side are connected with the 24 joint and the 10 joint on the top surface of the protractor disc one by one.

[0014] In an embodiment of the above device, the telescopic butt joint assembly comprises a sliding seat, a motor, a lead screw, a support and a self-centering unit; the sliding seat comprises two opposite ear seats, four limiting rods are arranged between the two ear seats, the four limiting rods pass through a sliding block, and the sliding block can slide on the limiting rods; the motor is composed of a stepping motor and a harmonic reducer; the output shaft of the motor is connected with a coupling, the end of the coupling is connected with a ball screw; the lead screw is inserted into the center of the sliding block and engaged with the sliding block; the support comprises a U-shaped plate and high-strength vertical plates perpendicular to the two ends of the U-shaped plate; the center of the bottom surface of the U-shaped plate is fixedly connected with the sliding block; and one self-centering unit is arranged on each vertical plate.

[0015] In an embodiment of the above device, the rear cover of the motor is internally provided with an expansion compensation gasket, a pressure compensation film and an oil injection port; the oil injection port comprises a plug, a plug seat and a plug nut, the plug is placed in the plug seat and is connected to the rear cover through the plug nut.

[0016] In an embodiment of the above device, the self-centering unit comprises a pair of symmetrically arranged elastic ball membranes and a ball head cover, which is connected to the vertical plate through the ball head cover; among the two self-centering units, one self-centering unit is connected to the inner side of the protractor through a butt joint, and the butt joint mouth is a ball head injection mouth; the other self-centering unit is connected to the outer side of the protractor through a butt joint, and the butt joint mouth is a ball head filter mouth.

[0017] The beneficial effects of the present application are as follows:

[0018] 1. The device can process a larger batch of samples at the same time through the configuration of multiple independent sampling cup units, thereby improving the sample processing capacity; at the same time, these units have an equal-interval staggered arrangement, which can more efficiently remove impurities and improve filtering precision and sample purity.

[0019] 2. The device is divided into multiple functional modules, which is convenient for daily maintenance and regular replacement of parts; this not only improves the reliability of the equipment, but also greatly prolongs its service life, and is particularly suitable for carrying and transporting underwater carriers in scientific research experiments;

[0020] 3. The planetary gear transmission assembly can accurately control the action of each sampling cup unit, and the telescopic butt joint assembly is driven by the movement of the motor, the bracket and the self-centering unit; each component makes the device more accurate and efficient during operation, greatly improving the accuracy and stability of microbial enrichment. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 It is a perspective structural schematic diagram of an embodiment of the present application.

[0022] Figure 2 It is a front view structural schematic diagram of Figure 1 .

[0023] Figure 3 It is a front view cross-sectional structural schematic diagram of Figure 1 .

[0024] Figure 4 It is a structural schematic diagram of the telescopic butt joint assembly in Figure 1 .

[0025] Figure 5 It is a cross-sectional structural schematic diagram of Figure 4 . DETAILED DESCRIPTION

[0026] The related technical solutions will be clearly and completely described below in combination with the drawings in the embodiments of the utility model, and obviously, the described embodiments are only part of the embodiments, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the ordinary skilled in the art without creative labor belong to the range protected by the utility model.

[0027] As shown in Figure 1 The long-time sequence microorganism enrichment device disclosed by the embodiment comprises a sampling cup group 1 and a pipeline butt joint system.

[0028] The sampling cup group 1 is used for enrichment, storage and impurity filtration of microorganisms, and the pipeline butt joint system comprises a planetary gear transmission assembly 2, a telescopic butt joint assembly 3 and a pump water assembly, and the injection, filtration and discharge of liquid are completed.

[0029] The sampling cup group 1 is composed of multiple independent sampling cup units, each of which is arranged above a protractor in an equidistant staggered manner to improve the filtration efficiency and sample purity; each sampling cup has a cup cover that can be independently opened, facilitating sample extraction after the enrichment work is completed.

[0030] Two types of pipelines are arranged on the sampling cup, which are respectively connected to the 24 connectors and 10 connectors on the top surface of the protractor to ensure the smoothness of liquid injection and discharge.

[0031] As shown in Figure 2 And Figure 3 The planetary gear transmission assembly 2 is used for controlling the position and butt joint action of the sampling cup unit, and the components thereof comprise a base 21, a tray 22, a rotating disc support ring 23, a planetary gear pair 24 and a protractor 25 in the assembly relationship from bottom to top.

[0032] The base 21 is a circular thick seat, and the tray 22 is a same disc, which is supported above the base by a plurality of support rods arranged at a circumferential interval. The tray is provided to provide a stable structure of the whole machine.

[0033] The rotating disc support ring 23 is arranged on the tray 22, the upper and lower end faces of the rotating disc support ring 23 can rotate relative to the center, and the lower end face of the rotating disc support ring is fixed to the tray.

[0034] The planetary gear pair 24 comprises an outer ring gear ring and a cylindrical gear which are meshed with each other, the outer ring gear ring is fixed to the upper end face of the rotating disc support ring, the bottom end of the cylindrical gear is connected with the output shaft of a rotating motor, and the rotating motor is fixed to the base 21. The rotating motor can drive the cylindrical gear to rotate.

[0035] The index plate 25 is fixed on the outer ring gear ring, and a group of equal-interval circumferentially arranged butt joints and plugs are arranged on the outer side of the index plate, and a group of equal-interval circumferentially arranged butt joints are arranged on the inner side of the index plate, and the two groups of butt joints are consistent in height and are located above the plugs. The butt joints on the outer side and the inner side are respectively communicated with the 24 joints and the 10 joints on the top surface of the index plate one by one.

[0036] Through the planetary gear transmission assembly, the index plate can rotate accurately, and the corresponding sampling cup and the pipeline butt joint system can be matched in position.

[0037] As shown in Figure 4 and Figure 5 , the telescopic butt joint assembly 3 includes a sliding seat 31, a motor 32, a lead screw 33, a support 34 and a self-centering unit 35.

[0038] The sliding seat 31 includes two opposite ear seats, and four limiting rods are arranged between the two ear seats. The four limiting rods pass through a sliding block, and the sliding block can slide on the limiting rods.

[0039] The motor 32 is composed of a step motor and a harmonic reducer. The output shaft of the motor is connected to a shaft coupling, and the end of the shaft coupling is connected to a ball screw 33. The lead screw is inserted into the center of the sliding block and engaged therewith. By rotating the motor to drive the shaft coupling and the lead screw to rotate, the sliding block can be controlled to slide along the limiting rods.

[0040] The rear cover of the motor 32 is internally provided with a telescopic compensation gasket, a pressure compensation film and an oil filling port. The oil filling port includes a plug, a plug seat and a plug nut, the plug is placed in the plug seat, and the plug nut is connected to the rear cover.

[0041] The support 34 includes a U-shaped plate and two high-strength vertical plates perpendicular to the two ends of the U-shaped plate. The center of the bottom surface of the U-shaped plate is fixedly connected with the sliding block.

[0042] Each of the two vertical plates is provided with a self-centering unit 35. The self-centering unit includes a pair of symmetrically arranged elastic ball membranes and a ball head cover, which is connected with the vertical plate through the ball head cover.

[0043] The two self-centering units 35 are different in that the self-centering unit that is connected with the inner side butt joint of the index plate 25 is a ball head injection nozzle, and the self-centering unit that is connected with the outer side butt joint of the index plate is a ball head filter tip, and the corresponding tracheal joint specifications of the two are different.

[0044] The pump assembly includes two pairs of water pump motors and a flow meter arranged on the pump water pipeline, and the two pump water pipelines are connected with the input ends of the two self-centering units 35. The pump assembly is arranged to pump the liquid to be treated into the pipeline in the index plate through the self-centering assembly, and finally pump the liquid into the sampling cup group.

[0045] The application method of the device is as follows:

[0046] 1. Assemble and check the device, make sure the sampling cup group, pipeline butt joint system, planetary gear transmission assembly and telescopic butt joint assembly are installed in place;

[0047] 2. Start the device, use the planetary gear transmission assembly to align the self-centering assembly of the sampling cup group with the corresponding butt joint interface on the index plate, ensure accurate connection;

[0048] 3. Drive the slider by the motor, drive the bracket and self-centering unit to move linearly, make the ball head injection nozzle accurately butt joint with the butt joint interface on the index plate;

[0049] 4. After the butt joint of the ball head injection nozzle is completed, the pump water assembly injects seawater into the sampling cup group along the pipeline passage, then repeat the seawater injection work until the sampling cup group is fully injected with seawater;

[0050] 5. After the seawater injection is completed, the motor is reversely driven until the ball head filter nozzle on the self-centering assembly is accurately butt joint with the corresponding butt joint interface on the index plate;

[0051] 6. After the butt joint of the ball head filter nozzle is completed, the pump water assembly injects fixing liquid into the sampling cup along the pipeline passage, then repeat the fixing liquid injection work until the sampling cup group is fully injected with fixing liquid for microbial filtration and enrichment work;

[0052] 7. After the biological filtration and enrichment work is completed, the sampling cup cover can be opened for sampling.

[0053] Finally, it should be noted that the above only for the preferred embodiments of the present application, and not for limiting the present application, although a detailed description is made with reference to the foregoing embodiments, for those skilled in the art, it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for some technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A long time-series microorganism enrichment device, characterized by: It includes a sampling cup group, a planetary gear transmission assembly, a telescopic docking assembly and a water pumping assembly; The sampling cup group is composed of a plurality of independent sampling cup units, each sampling cup unit is arranged at an equal interval on a protractor, and each sampling cup unit has an independently opened cup cover; a plurality of docking interfaces are arranged on the inner side and the outer side of the protractor at a fixed interval, and each docking interface is connected with each sampling cup unit; The protractor is driven by the planetary gear transmission assembly and can rotate along the center of the circle; The telescopic docking assembly drives the displacement of the sliding block, a support is arranged on the sliding block, and self-centering units are arranged at both ends of the support and can be docked with the docking interfaces on the protractor; The water pumping assembly includes a water pump motor and a flowmeter arranged on the water pumping pipeline, and the non-docking sides of the two self-centering units are respectively connected with the water pumping pipelines of the water pumping assembly.

2. The long-term microbial enrichment device of claim 1, wherein: Two pipelines are arranged on the sampling cup unit and correspondingly connected with the 24 joint and the 10 joint on the top surface of the protractor.

3. The long-term microbial enrichment device of claim 2, wherein: The planetary gear transmission assembly includes a base, a tray, a rotating disc support ring, a planetary gear pair and a protractor; The base is a circular thick seat, the tray is a same disc, and the tray is supported above the base by a plurality of support rods arranged at an interval along the circumference; the rotating disc support ring is arranged on the tray, the upper and lower end faces of the rotating disc support ring can rotate relative to each other along the center of the circle, and the lower end face of the rotating disc support ring is fixed to the tray; The planetary gear pair includes an outer ring gear ring and a cylindrical gear which are meshed with each other, the outer ring gear ring is fixed to the upper end face of the rotating disc support ring, the bottom end of the cylindrical gear is connected with the output shaft of a rotating motor, and the rotating motor is fixed to the base; and the protractor is fixed to the outer ring gear ring.

4. The long-term microbial enrichment device of claim 3, wherein: A group of docking interfaces and plugs arranged at an equal interval along the circumference are respectively arranged on the outer side of the protractor, and a group of docking interfaces arranged at an equal interval along the circumference are arranged on the inner side of the protractor, the heights of the two groups of docking interfaces are consistent and both are above the plugs; the docking interfaces on the outer side and the inner side are respectively connected with the 24 joint and the 10 joint on the top surface of the protractor.

5. The long-term microbial enrichment device of claim 1, wherein: The telescopic docking assembly includes a sliding seat, a motor, a lead screw, a support and a self-centering unit; The sliding seat includes two opposite ear seats, four limiting rods are arranged between the two ear seats, the four limiting rods pass through a sliding block, and the sliding block can slide on the limiting rods; The motor is composed of a step motor and a harmonic reducer; the output shaft of the motor is connected with a coupling, and the end of the coupling is connected with a ball screw; the ball screw is inserted into the center of the sliding block and engaged with the sliding block; The support includes a U-shaped plate and high-strength vertical plates perpendicular to both ends of the U-shaped plate; the bottom surface center of the U-shaped plate is fixedly connected with the sliding block; one self-centering unit is arranged on each vertical plate.

6. The long-term microbial enrichment device of claim 5, wherein: A telescopic compensation gasket, a pressure compensation film and an oil injection port are arranged in the rear cover of the motor; the oil injection port includes a plug, a plug seat and a plug nut, the plug is placed in the plug seat, and the plug nut is connected to the rear cover.

7. The long-term microbial enrichment device of claim 5, wherein: The self-centering unit includes a pair of symmetrically arranged elastic ball membranes and a ball head cover, and the ball head cover is connected with the vertical plate; in the two self-centering units, one self-centering unit is docked with the inner side docking interface of the protractor, and the docking mouth thereof is a ball head injection mouth; the other self-centering unit is docked with the outer side docking interface of the protractor, and the docking mouth thereof is a ball head filter tip.

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