Sample collecting and processing device
By designing a sample collection and processing device that stores preservation solution and adsorbent separately, and utilizing a disconnection and filtration component, the complex problems in PCR testing of fecal samples were solved, achieving efficient and accurate sample processing and testing.
Patent Information
- Application Number
- CN202423162638.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-12-12
- Estimated Expiration
- 2034-12-20
AI Technical Summary
Existing technologies for PCR detection of fecal samples suffer from problems such as difficulty in breaking down Gram-positive bacteria, residual substances inhibiting downstream experiments, and PCR inhibitors in fecal samples, resulting in complex experimental procedures and low efficiency.
Design a sample collection and processing device, comprising a first chamber and a second chamber, which respectively store a preservation solution and an adsorbent. The two are isolated before use and connected during detection by a disconnection component. Impurities are filtered out by a filter component, simplifying the operation process.
It reduces experimental steps, improves experimental efficiency and reliability, ensures the accuracy of PCR detection, avoids inhibition, and simplifies sample preservation and transportation.
Smart Images
Figure CN223660079U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to sample detection technical field, especially a kind of sample collection and processing device. BACKGROUND
[0002] Helicobacter pylori is a common stomach infection bacteria, about 50% of the population in the world is infected with this bacteria, and the bacteria is mainly transmitted through oral route, such as dining together. At present, the infection rate of Helicobacter pylori is high, and it is considered as one of the main causes of gastric cancer, and eradication of Helicobacter pylori is regarded as an important measure to prevent gastric cancer. Helicobacter pylori PCR detection can accurately detect Helicobacter pylori infection in feces, and the detection can also identify mutations that may produce antibiotic resistance. In addition, PCR detection does not require strict pre-analysis conditions and can be completed within a few hours, making it a rapid detection method.
[0003] The sample types of Helicobacter pylori PCR detection include gastric tissue, feces, gastric secretions and other samples. Fecal samples are one of non-invasive samples, and PCR detection through fecal samples can detect Helicobacter pylori DNA, which is a convenient and highly acceptable method for patients. However, there are problems such as difficulty in breaking the wall of gram-positive bacteria, inhibition of downstream experiments by residual substances, and the presence of various PCR inhibitors in fecal samples during nucleic acid extraction of fecal samples. The existing technology uses a small spoon-shaped collection device to collect part of the feces from the surface of the feces. This method requires post-experimental personnel to perform high-speed centrifugation and other operations, increasing the difficulty of subsequent experiments. UTILITARY MODEL CONTENT
[0004] The utility model aims at the deficiencies in the above background art, and provides an improved sample collection and processing device to reduce inhibitors in fecal samples or similar samples, reduce experimental operation steps, and improve experimental efficiency and reliability.
[0005] To achieve the above purpose, the utility model provides a kind of sample collection and processing device, including first cavity and second cavity, the first cavity is provided with preservative, the second cavity is provided with adsorbent and filter component, the first cavity with the second cavity is provided with disconnecting component;
[0006] When sample is not put into the first cavity and sample is saved in the first cavity, the disconnecting component disconnects the first cavity and the second cavity, when PCR detection is needed for sample, the disconnecting component makes the first cavity and the second cavity communicate.
[0007] The filter assembly is arranged inside the second cavity, or arranged inside the first cavity simultaneously, and used for filtering the mixed solution in the first cavity when the mixed solution enters the second cavity, and filtering the mixed solution in the second cavity after the impurities are adsorbed and removed when the mixed solution is led out.
[0008] Further, the preservation liquid is preloaded in the first cavity, and the adsorbent is preloaded in the second cavity.
[0009] Further, the first end and the second end of the first cavity, and the first end and the second end of the second cavity are provided with openings, the first end of the first cavity is used for putting in the fecal sample, and is provided with an end cover which is detachably connected with the first end of the first cavity, the second end of the first cavity is connected with the first end of the second cavity, and the on-off assembly is arranged.
[0010] Further, the on-off assembly comprises a barrier and a piercer, the barrier is arranged at the second end of the first cavity, the piercer is arranged at the first end of the second cavity, and the piercer can pierce the barrier when moving towards the barrier, so that the second end of the first cavity is communicated with the first end of the second cavity.
[0011] Further, the second end of the first cavity is provided with external threads, the first end of the second cavity is provided with matching internal threads, and the second end of the first cavity is inserted into the second cavity and threadedly connected.
[0012] Further, the second end of the first cavity is provided with a connecting ring which is connected with the first end of the second cavity simultaneously, and the connecting ring can be damaged after a preset torsion force is applied, so that the first cavity can rotate relative to the second cavity.
[0013] Further, the filter assembly comprises a filter screen and a filter membrane, the filter screen is arranged at the first end of the second cavity and used for filtering the mixed solution in the first cavity, and the filter membrane is arranged at the second end of the second cavity, the filter aperture of the filter membrane is smaller than the filter screen and smaller than the particle size of the adsorbent.
[0014] Further, the end cover is threadedly connected or elastically connected with the first cavity.
[0015] Further, the sample collection and processing device further comprises a stirring assembly which is connected with the end cover and used for stirring the first cavity.
[0016] Further, the stirring assembly comprises a stirring shaft and stirring blades, the second end of the stirring shaft is connected with the end cover, and the stirring blades are connected with the first end and the middle part of the stirring shaft.
[0017] The above scheme of the utility model has the following beneficial effects:
[0018] The sample collection and processing device provided by the utility model can store the preservative liquid and the adsorbent of impurities such as adsorption inhibitors in the first cavity and the second cavity respectively in advance, and through the arrangement of the on-off assembly, the preservative liquid in the first cavity and the adsorbent in the second cavity are prevented from mixing in advance before detection, the sample preservation and adsorption impurity removal effect are affected, the reagent before sampling, the sample preservation after sampling and the transportation process can be stable, the accuracy of PCR detection can be ensured, there is no inhibitory effect, and centrifugation operation is not needed, therefore, the experimental operation steps are reduced, and the experimental efficiency and reliability are improved.
[0019] Other beneficial effects of the utility model will be described in detail in the following specific embodiment part. BRIEF DESCRIPTION OF DRAWINGS
[0020] Figure 1 It is an overall structure schematic view of the utility model;
[0021] Figure 2 It is an overall structure split schematic view of the utility model;
[0022] Figure 3 It is a first cavity and a second cavity internal schematic view of the utility model;
[0023] Figure 4 It is an on-off assembly schematic view of the utility model.
[0024]
Explanation of reference signs
[0025] 1-first cavity;2-second cavity;3-preservation liquid;4-adsorbent;5-end cover;6-barrier;7-piercer;8-connection ring;9-filter screen;10-filter membrane;11-stirring shaft;12-stirring blade. DETAILED DESCRIPTION
[0026] In order to make the technical problems, technical schemes and advantages to be solved by the utility model more clear, the following will be described in detail in combination with the drawings and specific embodiments. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model. In addition, the technical features involved in different embodiments of the utility model described below can be combined with each other as long as they do not conflict with each other.
[0027] In the description of the utility model, it needs to explain, the term "center", "upper", "lower", "left", "right", "vertical", "horizontal", "internal", "external" and so on indicate the orientation or position relation based on the orientation or position relation shown in the drawing, only for the convenience of describing the utility model and simplifying the description, and not indicate or imply that the device or element indicated must have a particular orientation, a particular orientation and operation, therefore, it cannot be understood as the limitation of the utility model. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.
[0028] In the description of the utility model, it needs to explain, the term "center", "upper", "lower", "left", "right", "vertical", "horizontal", "internal", "external" and so on indicate the orientation or position relation based on the orientation or position relation shown in the drawing, only for the convenience of describing the utility model and simplifying the description, and not indicate or imply that the device or element indicated must have a particular orientation, a particular orientation and operation, therefore, it cannot be understood as the limitation of the utility model. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.
[0029] As Figures 1-3 The utility model discloses an embodiment provides a kind of sample collection and processing device, for fecal sample and similar sample, including the first cavity 1 and second cavity 2 that can be interconnected.Wherein, the first cavity 1 is as sample storage cavity, for storing preservative 3, to make the fecal sample collected in preservative 3 storage. Second cavity 2 is as adsorption impurity removal cavity, adsorbent 4 and filter assembly are provided in second cavity 2. It needs to be explained, preservative 3 is a kind of reagent widely used for the preservation of fecal sample under normal temperature condition, which can ensure the stability and integrity of specimen DNA in sample, so as to ensure that high-quality DNA is used for subsequent PCR genetic detection experiment. The main role of preservative 3 is to stop the degradation process of DNA immediately after sampling, after collecting fecal sample, cells in fecal sample will release DNA rapidly, and enzyme and bacterial activity in fecal sample will lead to the degradation of DNA. Therefore, using preservative 3 can effectively protect the DNA in fecal sample, so that it remains stable during storage and transportation. Adsorbent 4 is used to further adsorb inhibitor in sample, so when sample (mixed solution after being mixed with preservative 3) passes through adsorbent 4, inhibitor in sample can be further adsorbed, so that subsequent PCR detection can be directly carried out without high-speed centrifugation process.
[0030] In consideration of the fact that the preservative solution 3 needs to be separated from the adsorbent 4 when it is not mixed with the fecal sample sufficiently (or when it is not used), so as to avoid affecting the integrity of the functionality, in this embodiment, the two reagents are arranged in the first cavity 1 and the second cavity 2 respectively, and the first cavity 1 and the second cavity 2 are not connected when not in use and during sample preservation. When the sample needs to be detected by PCR, the mixed solution is introduced into the second cavity 2 to mix with the adsorbent 4, so as to adsorb the impurities such as inhibitors before PCR detection.
[0031] In consideration of the convenience of the entire device as a product, in this embodiment, the first cavity 1 and the second cavity 2 are connected in an integrated manner rather than being independent components. The connection part of the first cavity 1 and the second cavity 2 is provided with a break-through assembly, which disconnects the first cavity 1 and the second cavity 2 when the device is not in use and the first cavity 1 is used to preserve the fecal sample, and connects the first cavity 1 and the second cavity 2 when the sample needs to be detected by PCR, so that the solution enters the second cavity 2 to adsorb the impurities for PCR detection.
[0032] This arrangement makes the use of the device more convenient. The preservative solution 3 and the adsorbent 4 are preloaded into the first cavity 1 and the second cavity 2 as part of the product, without the need for subsequent addition, which simplifies the operation steps of the experimental personnel, avoids cross contamination, and keeps the sample stable during sampling, preservation and transportation. The preservative solution 3 and the mixed solution after sampling will not mix with the adsorbent 4 in the second cavity 2.
[0033] As a preferred embodiment, in this embodiment, the first cavity 1 and the second cavity 2 are both arranged in a cylindrical shape, so that the entire device is slender, which is convenient for preservation and use. The first end and the second end of the first cavity 1 and the first end and the second end of the second cavity 2 are all provided with openings. Among them, the first end of the first cavity 1 is used for the input of the fecal sample, and an end cover 5 is configured, which is detachably connected with the first end of the first cavity 1, so as to be opened when the sample is input, and closed and sealed during sample preservation and transportation. At the same time, as shown in the figure, the break-through assembly includes a barrier 6 and a piercer 7. The barrier 6 is arranged at the second end of the first cavity 1, which seals the second end of the first cavity 1 in the intact state, so that the preservative solution 3 or the mixed solution in the first cavity 1 will not enter the second cavity 2. The piercer 7 is arranged at the first end of the second cavity and has a sharp end. When the piercer 7 is controlled to move towards the barrier 6, the piercer 7 can pierce the barrier 6 to open the second end of the first cavity 1, so that the mixed solution enters the second cavity 2 for adsorption and removal of impurities. Figure 4
[0034] It should be noted that the barrier 6 can be in a film or plate structure, preferably in a plate structure with a certain hardness (similar to the sealing of a barrel of water), and the piercer 7 is made of a material with greater hardness, so that after the tip of the piercer 7 contacts and presses the barrier 6, the barrier 6 can be fully opened, avoiding the unsmooth introduction of the mixed solution into the second cavity 2.
[0035] At the same time, considering the integrated connection of the second cavity 2 with the first cavity 1 and the need to move relative to the first cavity 1, in this embodiment, the second end of the first cavity 1 is provided with external threads, and the first end of the second cavity 2 is provided with matching internal threads, both of which are in threaded connection, and the second end of the first cavity 1 is inserted into the second cavity 2. Therefore, under normal conditions, the first cavity 1 and the second cavity 2 are in threaded connection, and the depth of the threaded connection is the initial depth, at which time the piercer 7 has not contacted the barrier 6. When the first cavity 1 or the second cavity 2 is twisted, the first cavity 1 can be further moved into the second cavity 2, so that the piercer 7 at the first end of the second cavity 2 contacts and pierces the barrier 6.
[0036] As a preferred embodiment, in this embodiment, a connecting ring 8 is also fixedly arranged at the second end of the first cavity 1, and the connecting ring 8 is fixedly connected with the first end of the second cavity 2. The connecting ring 8 is in a cross-sectional form, so that after a certain torsion is applied, it can be broken, so that the first cavity 1 can be twisted relative to the second cavity 2, while remaining stable in the storage and transportation state, avoiding the piercer 7 from prematurely piercing the barrier 6 and affecting storage.
[0037] In this embodiment, the filter assembly includes a filter screen 9 and a filter membrane 10. The filter screen 9 is arranged in the second cavity 2 near the first end, for filtering the mixed solution in the first cavity 1, so that large-particle impurities in the mixed solution do not enter the second cavity 2, avoiding affecting the adsorption and impurity removal effect. At the same time, considering that the adsorbent 4 is in a solid particle form, in this embodiment, the second end of the second cavity 2 is arranged in an open form, and the filter membrane 10 is arranged in the second cavity 2 near the second end. The filter size of the filter membrane 10 is significantly smaller than that of the filter screen 9, for filtering the mixed solution after being adsorbed by the adsorbent 4, so that the liquid-shaped fecal sample can flow out from the second end of the second cavity 2 after being filtered, and after being collected, PCR detection can be performed. The adsorbent 4 that has adsorbed impurities and small-particle impurities cannot pass through the filter membrane 10, but remain in the second cavity 2, which can be recycled as waste garbage later. Since the adsorbent 4 itself cannot pass through the filter membrane 10, the second end of the second cavity 2 does not need to be provided with an end cover 5 or the like, and the adsorbent 4 can be pre-loaded into the second cavity 2 and stored through the filter membrane 10.
[0038] Of course, as a preferred embodiment, the filter screen 9 and the filter membrane 10 of the filter assembly can be provided with multiple layers, and each layer of the filter screen 9 or the filter membrane 10 is divided into different pore sizes, etc., so as to further improve the hierarchical filtering effect.
[0039] It is worth mentioning that when the collected fecal sample is mixed with the preservation liquid 3 in the first cavity 1, there may be uneven mixing, and it is difficult to ensure that the center of the large fecal sample is fully mixed with the preservation liquid 3 by simply relying on the shaking device. Based on this, the stirring assembly is further provided in the embodiment, which specifically includes a stirring shaft 11 and stirring blades 12 provided at the first end and the middle of the stirring shaft 11, and the second end of the stirring shaft 11 is connected with the end cover 5. Therefore, after the fecal sample is put into the first cavity 1, the first end of the stirring shaft 11 can be inserted into the first cavity 1 by holding the end cover 5, and then the end cover 5 is rotated to stir the fecal sample, so that the fecal sample is fully mixed with the preservation liquid 3.
[0040] In one specific embodiment, the end cover 5 and the first end of the first cavity 1 are also connected in a threaded manner (and sealed by a sealing ring), and when the device is not in use, the end cover 5 is closed at the first end of the first cavity 1, and the stirring shaft 11 is also stored by being inserted into the first cavity 1. When the fecal sample needs to be preserved, the end cover 5 is unscrewed and the stirring shaft 11 is extracted at the same time, the stirring shaft 11 is inserted by holding the end cover 5 after the fecal sample is put in. At this time, the end cover 5 is not tightened, and the stirring blades 12 can stir the first cavity 1, and then the end cover 5 is tightened after completion. In other specific embodiments, the end cover 5 and the first end of the first cavity 1 can also be elastically matched and sealed by plugging.
[0041] In addition, the stirring shaft 11 can also be connected with the end cover 5 in a rotating manner, and a knob is provided on the end cover 5, the knob is connected with the end cover 5 in a rotating manner, and the second end of the stirring shaft 11 is fixedly connected with the knob. Therefore, the first end of the first cavity 1 can be sealed by the end cover 5, and then the knob is twisted to rotate the stirring shaft 11. Of course, this method may cause the first cavity 1 to leak, so it is only an optional embodiment.
[0042] In summary, after the end cover 5 is opened, the fecal sample is put into the first cavity 1, then the first cavity 1 is stirred by the stirring assembly connected with the end cover 5, so that the fecal sample is fully mixed with the preservative 3, then the end cover 5 is closed to seal the first cavity 1, so that the fecal sample is preserved, transported and the like. When PCR detection is needed, the second cavity 2 is rotated relative to the first cavity 1, so that the piercer 7 contacts and pierces the barrier 6, so that the mixed solution in the first cavity 1 is transferred into the second cavity 2, and the large impurities are filtered by the filter screen 9, the mixed solution is adsorbed by the adsorbent 4 after being purified in the second cavity 2, and then directly flows out from the second end of the second cavity 2, and after collection, the PCR detection can be directly performed, and through the collection and the adsorption of the inhibitors before detection, the accuracy of the PCR detection can be ensured, and there is no inhibition effect, and no centrifugation operation is needed.
[0043] It should be noted that the sample collection and processing device provided in the embodiment is not only suitable for fecal samples, but also other similar samples that need to be preserved through corresponding steps, and thus belongs to the protection scope of the present application.
[0044] The technical features of the above embodiments can be combined arbitrarily, and in order to make the description simple, all possible combinations of the technical features in the above embodiments are not described, however, as long as the combination of the technical features does not exist contradictory, it should be considered as the scope of the present application.
[0045] The above embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as the limitation of the application scope. It should be noted that for those skilled in the art, without departing from the concept of the present application, several modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.
Claims
1. A sample collection and processing device, characterized by, It comprises a first cavity (1) and a second cavity (2), the first cavity (1) is provided with a preservation liquid (3), the second cavity (2) is provided with an adsorbent (4) and a filter assembly, a break-through assembly is arranged between the first cavity (1) and the second cavity (2); When the sample is not put into the first cavity (1) and the sample is preserved in the first cavity (1), the break-through assembly disconnects the first cavity (1) and the second cavity (2), when the sample needs to be detected by PCR, the break-through assembly connects the first cavity (1) and the second cavity (2); The filter assembly is arranged in the second cavity (2) or in the first cavity (1) at the same time, the filter assembly is used for filtering when the mixed solution in the first cavity (1) enters the second cavity (2) and filtering when the mixed solution in the second cavity (2) after impurity removal is guided out.
2. The sample collection and processing device of claim 1, wherein, The preservation liquid (3) is preloaded into the first cavity (1), and the adsorbent (4) is preloaded into the second cavity (2).
3. The sample collection and processing device of claim 1, wherein, The first end and the second end of the first cavity (1) and the first end and the second end of the second cavity (2) are provided with openings, the first end of the first cavity (1) is used for putting in the fecal sample, and an end cover (5) is arranged at the same time, the end cover (5) is detachably connected with the first end of the first cavity (1), the second end of the first cavity (1) is connected with the first end of the second cavity (2), and the break-through assembly is arranged.
4. The sample collection and processing device of any one of claims 1-3, wherein, The break-through assembly comprises a barrier (6) and a piercer (7), the barrier (6) is arranged at the second end of the first cavity (1), the piercer (7) is arranged at the first end of the second cavity (2), and when the piercer (7) moves towards the barrier (6), the barrier (6) can be pierced to connect the second end of the first cavity (1) and the first end of the second cavity (2).
5. The sample collection and processing device of claim 4, wherein, The second end of the first cavity (1) is provided with external threads, the first end of the second cavity (2) is provided with matching internal threads, and the second end of the first cavity (1) is inserted into the second cavity (2) and threadedly connected.
6. The sample collection and processing device of claim 5, wherein, The second end of the first cavity (1) is provided with a connecting ring (8), the connecting ring (8) is connected with the first end of the second cavity (2) at the same time, and the connecting ring (8) can be damaged after a preset torsion force is applied, so that the first cavity (1) can rotate relative to the second cavity (2).
7. The sample collection and processing device of claim 1, wherein, The filter assembly comprises a filter screen (9) and a filter membrane (10), the filter screen (9) is arranged at the first end of the second cavity (2) and used for filtering the mixed solution in the first cavity (1), and the filter membrane (10) is arranged at the second end of the second cavity (2), the filter aperture of the filter membrane (10) is smaller than that of the filter screen (9) and smaller than the particle size of the adsorbent (4).
8. The sample collection and processing device of claim 3, wherein, The end cover (5) is threadedly connected or elastically connected with the first cavity (1).
9. The sample collection and processing device of claim 8, wherein, The sample collection and processing device further comprises a stirring assembly, the stirring assembly is connected with the end cover (5), and the stirring assembly is used for stirring the first cavity (1).
10. The sample collection and processing device of claim 9, wherein, The stirring assembly comprises a stirring shaft (11) and stirring blades (12), the second end of the stirring shaft (11) is connected with the end cover (5), and the first end and the middle part of the stirring shaft (11) are connected with the stirring blades (12).