Fluid system and sequencing system

Abstract

The utility model discloses a fluid system and a sequencing system. The fluid system comprises a reaction device, a pipeline and a degassing device, the reaction device is used for providing a place for biochemical reaction, the pipeline is communicated with the reaction device and used for conveying liquid to the reaction device and / or receiving liquid flowing out of the reaction device, and the degassing device is connected with the pipeline and used for removing gas in the liquid in the pipeline. Therefore, gas in the liquid in the pipeline is removed through the degassing device, and bubbles formed in the reaction device can be reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of gene sequencing, especially to a fluid system and sequencing system. BACKGROUND

[0002] Gene sequencing technology means the technical means for obtaining DNA or RNA base sequence by detection. At present, the dominant sequencing technology is high-throughput sequencing technology. In the sequencing platform for realizing high-throughput sequencing based on sequencing by synthesis, the approximate process of gene sequencing includes fixing the nucleic acid sample to be tested on the flow cell in the form of hybridization; using PCR amplification to make the nucleic acid sample to be tested form nucleic acid molecule clusters; then adding sequencing reagents, such as bases with fluorescent groups, polymerase and primers, etc., into the flow cell through the fluid system, combining the bases with fluorescent groups with the bases on the nucleic acid sample to be tested through the principle of base complementary pairing; finally, exciting the fluorescent groups to produce fluorescence through the optical imaging system and collecting the fluorescence to form an image, identifying the bases through the image, thereby realizing the base sequence determination of the nucleic acid sample to be tested.

[0003] However, if there is gas in the sequencing reagent and / or the nucleic acid sample to be tested, the gas will enter the flow cell along with the sequencing reagent and / or the nucleic acid sample to be tested, and then bubbles will be formed in the flow cell, thereby affecting the quality of the photograph and further affecting the sequencing quality. UTILITY MODEL CONTENT

[0004] The present application provides a fluid system and sequencing system to at least solve one of the technical problems existing in the prior art.

[0005] In one aspect, the present application provides a fluid system, which comprises a reaction device, a pipeline and a degassing device. The reaction device is used to provide a place for biochemical reaction. The pipeline is in communication with the reaction device and is used to deliver liquid to the reaction device and / or receive liquid flowing out of the reaction device. The degassing device is connected with the pipeline and is used to remove gas in the liquid in the pipeline.

[0006] In this way, the gas in the liquid in the pipeline can be removed by the degassing device, so that the formation of bubbles in the reaction device can be reduced or avoided.

[0007] In some embodiments, the reaction device comprises a liquid inlet and a liquid outlet.

[0008] The pipeline comprises a first pipeline and a second pipeline. The first pipeline is in communication with the liquid inlet, and the second pipeline is in communication with the liquid outlet. The first pipeline and / or the second pipeline is connected with the degassing device.

[0009] In some embodiments, the reaction device comprises an inlet and an outlet, the pipeline comprises a bypass pipeline, one end of the bypass pipeline is communicated with the inlet, and the other end of the bypass pipeline is communicated with the outlet, and the bypass pipeline is connected with the degassing device.

[0010] In some embodiments, the degassing device comprises a sealed chamber, the pipeline passes through the sealed chamber, and the part of the pipeline in the sealed chamber is capable of allowing the gas in the liquid in the pipeline to escape when the sealed chamber is under a negative pressure condition, so that the liquid in the pipeline is degassed after passing through the sealed chamber.

[0011] In some embodiments, the degassing device comprises a negative pressure device and an exhaust pipeline, one end of the exhaust pipeline is communicated with the sealed chamber, and the negative pressure device is arranged on the exhaust pipeline and is used to generate a negative pressure in the sealed chamber.

[0012] In some embodiments, the degassing device comprises a detector between the negative pressure device and the sealed chamber, the detector is communicated with the exhaust pipeline, and the detector is used to detect the air pressure in the sealed chamber through the exhaust pipeline.

[0013] In some embodiments, the degassing device comprises a multi-way valve arranged on the exhaust pipeline and between the detector and the negative pressure device, and the multi-way valve is capable of communicating the negative pressure device with the sealed chamber or the atmosphere.

[0014] In some embodiments, the negative pressure device is a pump.

[0015] In some embodiments, the detector is communicated with the exhaust pipeline through a three-way joint.

[0016] In another aspect, the application provides a sequencing system comprising the above fluid system.

[0017] The additional aspects and advantages of the present application will be partially given in the following description, and some will become apparent from the following description, or will be understood by those skilled in the art through the practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0018] The above and / or additional aspects and advantages of the present application will become apparent and more readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:

[0019] Figure 1 is a structural schematic diagram of a fluid system of an embodiment of the present application;

[0020] Figure 2 is a structural schematic diagram of a degassing device of an embodiment of the present application;

[0021] Figure 3 is a structural schematic diagram of a degassing device of an embodiment of the present application;

[0022] Figure 4is a flowchart of the method of the embodiment of the present application;

[0023] Figure 5 is a flowchart of the method of the embodiment of the present application;

[0024] Figure 6 is a flowchart of the method of the embodiment of the present application;

[0025] Figure 7 is a flowchart of the method of the embodiment of the present application.

[0026] The figure mark explanation: 100, fluid system;10, reaction device;11, liquid inlet;12, liquid outlet;20, pipeline;21, first pipeline;22, second pipeline;23, bypass pipeline;30, degassing device;31, closed chamber;32, negative pressure device;33, exhaust pipeline;34, detector;35, tee joint;36, multi-way valve;37, first degassing device;38, second degassing device;39, third degassing device. Specific implementation

[0027] The embodiment of the present application is described in detail below, and examples of the embodiment are shown in the drawings, wherein the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiment described below by referring to the drawings is exemplary and is only used to explain the present application, and cannot be understood as a limitation of the present application.

[0028] In the description of the present application, it is understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements indicated must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application. In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise specifically limited.

[0029] In the description of the utility model, it is necessary to explain that, unless there is definite stipulation and limitation, the terms "mount", "link", "connect" should be understood broadly, for example, it can be fixed connection, also can be detachable connection, or integrally connected, can be mechanical connection, also can be electrical connection or can communicate with each other, can be directly connected, also can be indirectly connected through intermediate medium, can be the communication of two elements or the interaction of two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to specific circumstances.

[0030] In the utility model, unless there is definite stipulation and limitation, the first feature is "on" or "under" the second feature, which can include direct contact between the first and second features, or indirect contact between the first and second features through another feature between them. Moreover, the first feature "on", "above" and "above" the second feature includes the first feature directly above and obliquely above the second feature, or only indicates that the first feature is higher than the second feature in horizontal height. The first feature "under", "below" and "below" the second feature includes the first feature directly below and obliquely below the second feature, or only indicates that the first feature is lower than the second feature in horizontal height.

[0031] The following disclosure provides many different embodiments or examples for implementing different structures of the utility model. In order to simplify the disclosure of the utility model, the components and settings of specific examples are described below. Of course, they are only examples, and the purpose is not to limit the utility model. In addition, the utility model can refer to the same number and / or reference letter in different examples, and such repetition is for the purpose of simplification and clarity, which itself does not indicate the relationship between the various embodiments and / or settings discussed. In addition, the utility model provides examples of various specific processes and materials, but those skilled in the art can realize the application of other processes and / or the use of other materials.

[0032] Please refer to Figure 1 The application provides a fluid system 100, which comprises a reaction device 10, a pipeline 20 and a degassing device 30, the reaction device 10 is used for providing a place for biochemical reaction, the pipeline 20 is communicated with the reaction device 10, the pipeline 20 is used for conveying liquid to the reaction device 10 and / or receiving liquid flowing out of the reaction device 10, and the degassing device 30 is connected with the pipeline 20, and the degassing device 30 is used for removing gas in the liquid in the pipeline 20.

[0033] In this way, the gas in the liquid in the pipeline 20 is removed by the degassing device 30, so that the formation of bubbles in the reaction device 10 can be reduced.

[0034] Specifically, the reaction device 10 can be a flow cell, which can also be referred to as a chip. The chip includes a plurality of fluid channels having a space for containing liquid, which can contain a sample to be tested and reagents, so that the sample to be tested and the reagents can undergo biochemical reactions.

[0035] The pipeline 20 can be a pipe for transporting liquid. The cross-sectional shape of the pipeline 20 can be circular. The length and diameter of the pipeline 20 can be set according to actual needs. The degassing device 30 can be connected to a plurality of pipelines 20 to remove gas in the liquid in the plurality of pipelines 20. The gas in the liquid can be gas originally present in the liquid, such as a certain inhibiting gas added to the reagent to keep the reagent stable, gas precipitated from the liquid due to excessive negative pressure of the pipeline 20, or gas dissolved in the liquid.

[0036] Please refer to Figure 1 In some embodiments, the reaction device 10 includes a liquid inlet 11 and a liquid outlet 12. The pipeline 20 includes a first pipeline 21 and a second pipeline 22. The first pipeline 21 is in communication with the liquid inlet 11. The second pipeline 22 is in communication with the liquid outlet 12. The first pipeline 21 and / or the second pipeline 22 is connected to the degassing device 30.

[0037] Specifically, the first pipeline 21 can be connected to the degassing device 30, the second pipeline 22 can be connected to the degassing device 30, or both the first pipeline 21 and the second pipeline 22 can be connected to the degassing device 30.

[0038] When delivering liquid to the reaction device 10, the liquid can be degassed by the degassing device 30 in the first pipeline 21 and then enter the reaction device 10 from the liquid inlet 11, or the liquid can be degassed by the degassing device 30 in the second pipeline 22 and then enter the reaction device 10 from the liquid outlet 12. In this way, the liquid can be degassed in advance before flowing into the reaction device 10, reducing or avoiding the formation of gas bubbles in the reaction device 10.

[0039] When receiving liquid flowing out of the reaction device 10, the liquid can flow out of the reaction device 10 from the liquid outlet 12, be degassed by the degassing device 30 in the second pipeline 22, and then be stored in the second pipeline 22. Then, the liquid can be degassed by the degassing device 30 in the second pipeline 22 and then enter the reaction device 10 from the liquid outlet 12 for reuse, reducing costs.

[0040] The liquid inlet 11 and the liquid outlet 12 are respectively located at two ends of the fluid channels of the reaction device 10. The number of fluid channels can be multiple. Each fluid channel includes one liquid inlet 11 and one liquid outlet 12. The number of first pipelines 21 and second pipelines 22 can be multiple. The plurality of fluid channels are arranged one-to-one with the plurality of first pipelines 21 and the plurality of second pipelines 22.

[0041] Please refer toFigure 1 In some embodiments, the pipeline 20 comprises a bypass pipeline 23, one end of the bypass pipeline 23 is in communication with the liquid inlet 11, and the other end is in communication with the liquid outlet 12, and the bypass pipeline 23 is connected with the degassing device 30.

[0042] Specifically, the end of the bypass pipeline 23 in communication with the liquid inlet 11 can be located between the liquid inlet 11 and the degassing device 30 of the first pipeline 21, and the degassing device 30 of the second pipeline 22 can be located between the end of the bypass pipeline 23 in communication with the liquid outlet 12 and the liquid outlet 12.

[0043] The liquid flowing out of the reaction device 10 can be stored in the bypass pipeline 23 after being degassed by the degassing device 30 of the second pipeline 22, then enter the first pipeline 21 after being degassed by the degassing device 30 of the bypass pipeline 23, enter the reaction device 10 from the liquid inlet 11, or enter the bypass pipeline 23 after being degassed by the degassing device 30 of the second pipeline 22, enter the first pipeline 21 after being degassed by the degassing device 30 of the bypass pipeline 23, and be stored in the first pipeline 21 or a storage container after being degassed by the degassing device 30 in the first pipeline 21, then enter the reaction device 10 from the liquid inlet 11 after being degassed by the degassing device 30 in the first pipeline 21. The liquid flows back from the bypass pipeline 23, which can reduce the flow resistance of the liquid and increase the flow rate of the liquid compared to the liquid flowing back from the second pipeline 22 to the first pipeline 21 through the reaction device 10, thereby improving the recovery efficiency of the liquid.

[0044] In the process of recycling and reusing the liquid, the liquid can be degassed by multiple degassing devices 30, thereby increasing the effective degassing area of the pipeline 20, improving the degassing effect, and at the same time reducing the length or area of the pipeline 20, reducing the use of materials, and thereby reducing the cost of the fluid system 100 and the flow resistance of the liquid.

[0045] Please refer to Figure 2 In some embodiments, the degassing device 30 comprises a sealed chamber 31, the pipeline 20 passes through the sealed chamber 31, and the part of the pipeline 20 located in the sealed chamber 31 can allow the gas in the liquid in the pipeline 20 to escape when the sealed chamber 31 is under negative pressure, so that the liquid in the pipeline 20 removes the gas after passing through the sealed chamber 31.

[0046] Specifically, the sealed chamber 31 can be a sealed space formed by polyvinyl chloride, and the sealed chamber 31 can be a cuboid, a cylinder, etc., and the pipeline 20 passing through the sealed chamber 31 can be the same side wall of the sealed chamber 31, or the pipeline 20 can pass through two adjacent side walls of the sealed chamber 31, or the pipeline 20 can pass through two opposite side walls of the sealed chamber 31.

[0047] The portion of the pipeline 20 located in the closed chamber 31 can be a Teflon tube, which has the characteristics of being permeable to gas and impermeable to water. The gas in the liquid can be discharged from the Teflon tube under the action of negative pressure when passing through the closed chamber 31, and the discharged gas is located in the closed chamber 31. The diameter of the Teflon tube can be consistent with the diameter of the first pipeline 21, the second pipeline 22 or the bypass pipeline 23, and the length of the Teflon tube can be set according to the content of the gas in the liquid. The negative pressure condition is the pressure range in which the gas in the closed chamber 31 can make the gas in the liquid in the pipeline 20 permeate out.

[0048] Referring to Figure 2 In some embodiments, the degassing device 30 includes a negative pressure device 32 and an exhaust pipeline 33, one end of the exhaust pipeline 33 communicates with the closed chamber 31, and the negative pressure device 32 is arranged on the exhaust pipeline 33 and is used to generate negative pressure in the closed chamber 31.

[0049] In this way, the negative pressure device 32 forms negative pressure in the closed chamber 31, so that the gas in the liquid in the pipeline 20 can be discharged after passing through the closed chamber 31.

[0050] Specifically, the negative pressure device 32 can be a vacuum pump, including but not limited to a water ring pump, a reciprocating pump, a slide valve pump, a rotary vane pump, a Roots pump and a diffusion pump. The negative pressure device 32 sucks the air in the closed chamber 31 to generate negative pressure in the closed chamber 31.

[0051] The exhaust pipeline 33 can transport the gas in the closed chamber 31 to the negative pressure device 32, so as to be discharged to the atmosphere through the negative pressure device 32, so that the negative pressure condition is formed in the closed chamber 31, and the gas in the closed chamber 31 can be the gas removed from the first pipeline 21, the second pipeline 22 or the bypass pipeline 23.

[0052] Referring to Figure 2 In some embodiments, the degassing device 30 includes a detector 34 located between the negative pressure device 32 and the closed chamber 31, the detector 34 communicates with the exhaust pipeline 33, and the detector 34 is used to detect the air pressure in the closed chamber 31 through the exhaust pipeline 33.

[0053] Specifically, the detector 34 can be a gas pressure sensor, which is a device for converting pressure into an electric signal by using pressure resistance, capacitance, piezoelectricity or crystal resonance. The detector 34 can communicate with the exhaust pipeline 33 through a three-way joint 35.

[0054] The detector 34 detects the air pressure in the sealed chamber 31 to determine whether the air pressure in the sealed chamber 31 reaches the negative pressure condition. When the air pressure in the sealed chamber 31 does not reach the negative pressure condition, the air removal device 30 is started. When the air pressure in the sealed chamber 31 reaches the negative pressure condition, the negative pressure device 32 is closed. By adjusting the working state of the negative pressure device 32, the sealed chamber 31 is maintained in the negative pressure state, thereby improving the air removal efficiency of the air removal device 30. Meanwhile, the opening and closing time of the negative pressure device 32 can be controlled, so that the use frequency of the negative pressure device 32 can be effectively reduced, thereby improving the service life of the negative pressure device 32.

[0055] Referring to Figure 3 In some embodiments, the air removal device 30 includes a multi-way valve 36 arranged on the exhaust pipeline 33 and located between the detector 34 and the negative pressure device 32. The multi-way valve 36 can connect the negative pressure device 32 to the sealed chamber 31 or the atmosphere.

[0056] Specifically, the multi-way valve 36 can be a three-way valve, and the three valve ports of the three-way valve are connected to the negative pressure device 32, the sealed chamber 31 and the atmosphere, respectively. In one embodiment, a negative pressure device 32 without a check function can be selected. When the negative pressure device 32 is working, the multi-way valve 36 connects the negative pressure device 32 and the sealed chamber 31, so that the negative pressure device 32 can suck the air in the sealed chamber 31 to form a negative pressure. When the negative pressure device 32 stops working, the multi-way valve 36 connects the negative pressure device 32 to the atmosphere, which can reduce the risk of the air pressure in the sealed chamber 31 being too high when the negative pressure device 32 is started, causing the negative pressure device 32 to be unable to start or difficult to start, thereby shortening the service life of the negative pressure device 32.

[0057] In another embodiment, a negative pressure device 32 with a one-way check function can be selected, which can reduce the use of solenoid valves, reduce the cost of the fluid system 100, and avoid the failure of the solenoid valve, thereby improving the reliability of the air removal device 30.

[0058] Referring to Figure 4 The present application provides a method for controlling the degassing of liquid in a fluid system 100, the method comprising:

[0059] S10, determining whether the air removal device 30 is normal;

[0060] S20, in the case that the air removal device 30 is normal, controlling the liquid in the pipeline 20 to pass through the air removal device 30 for degassing.

[0061] Specifically, the judgment of whether the degassing device 30 is normal can be performed before the fluid system 100 is started or during the operation of the fluid system 100. The degassing device 30 is normal means that all components in the degassing device 30 are normal, for example, the airtightness of the sealed chamber 31 is normal, the airtightness of the exhaust pipeline 33 is normal, the negative pressure device 32 works normally, and the detector 34 works normally. The degassing device 30 is abnormal means that one or more components in the degassing device 30 are abnormal, for example, the airtightness of the sealed chamber 31 is abnormal, the airtightness of the exhaust pipeline 33 is abnormal, the negative pressure device 32 works abnormally, or the detector 34 works abnormally.

[0062] In this way, by judging whether the degassing device 30 is normal, it is determined whether to start the degassing device 30.

[0063] Referring to Figure 5 In some embodiments, the judgment of whether the degassing device 30 is normal (step S10) comprises:

[0064] S100, obtaining the air pressure in the sealed chamber 31 by using the detector 34;

[0065] S200, judging whether the degassing device 30 is normal according to the change of the air pressure.

[0066] Specifically, before obtaining the air pressure in the sealed chamber 31 by using the detector 34, the detector 34 can be detected to ensure that the detector 34 is normal.

[0067] The change of the air pressure can be the change amount of the air pressure in a certain time, or the time difference for the air pressure to change by a certain amount. The judgment of whether the degassing device 30 is normal can be performed when the negative pressure device 32 is working, or when the negative pressure device 32 is stopped. In this way, the degassing device 30 can be monitored whether it is normal during the entire operation of the fluid system 100, so as to sufficiently remove the gas in the liquid in the pipeline 20.

[0068] Referring to Figure 6 In some embodiments, the judgment of whether the degassing device 30 is normal according to the change of the air pressure (step S200) comprises:

[0069] S210, obtaining a first air pressure in the sealed chamber 31 at an initial time;

[0070] S220, obtaining a second air pressure in the sealed chamber 31 at a preset time interval;

[0071] S230, calculating a change rate of the air pressure by using the first air pressure, the second air pressure, and the preset time interval;

[0072] S240, comparing the change rate of the air pressure with a preset change rate to judge whether the degassing device 30 is normal.

[0073] Specifically, the initial moment can be the moment when the negative pressure device 32 starts to work, or the moment when the negative pressure device 32 stops to work, and the preset time interval can be the length of time when the negative pressure device 32 works, or the length of time when the negative pressure device 32 stops to work.

[0074] The gas pressure change rate can be the ratio of the difference between the second gas pressure and the first gas pressure to the preset time interval, and the preset change rate is the change rate of the gas pressure in the sealed chamber 31 of the degassing device 30 in the preset time interval under normal conditions. The preset change rate can be a range, and when the gas pressure change rate is within the range, it is determined that the degassing device 30 is normal, and when the gas pressure change rate is outside the range, it is determined that the degassing device 30 is abnormal.

[0075] In this way, by calculating the change rate of the gas pressure in the preset time interval and comparing the gas pressure change rate with the preset change rate, it can be objectively and accurately determined whether the degassing device 30 is normal.

[0076] In an embodiment, the preset time interval gas pressure difference can be calculated by using the first gas pressure and the second gas pressure, and then the preset time interval gas pressure difference is compared with the preset time interval preset gas pressure difference to determine whether the degassing device 30 is normal.

[0077] In some embodiments, comparing the gas pressure change rate with the preset change rate to determine whether the degassing device 30 is normal includes:

[0078] When the negative pressure device 32 is working, if the gas pressure change rate is greater than or equal to the preset change rate, it is determined that the degassing device 30 is normal, and if the gas pressure change rate is less than the preset change rate, it is determined that the degassing device 30 is abnormal.

[0079] Specifically, when the negative pressure device 32 is working, if the gas pressure change rate is greater than or equal to the preset change rate, it means that the gas pressure in the sealed chamber 31 reaches the negative pressure condition in the preset time interval, and the gas tightness of the sealed chamber 31, the gas tightness of the exhaust pipeline 33 and the negative pressure device 32 are all normal, so it can be determined that the degassing device 30 is normal.

[0080] If the gas pressure change rate is less than the preset change rate, it means that the gas pressure in the sealed chamber 31 does not reach the negative pressure condition in the preset time interval, and the gas tightness of the sealed chamber 31, the gas tightness of the exhaust pipeline 33 or the negative pressure device 32 is abnormal, so it can be determined that the degassing device 30 is abnormal.

[0081] In some embodiments, comparing the gas pressure change rate with the preset change rate to determine whether the degassing device 30 is normal includes:

[0082] If the rate of change of the air pressure is less than or equal to the preset rate of change when the negative pressure device 32 stops working, it is determined that the air removal device 30 is normal; if the rate of change of the air pressure is greater than the preset rate of change, it is determined that the air removal device 30 is abnormal.

[0083] Specifically, if the rate of change of the air pressure is less than or equal to the preset rate of change when the negative pressure device 32 stops working, it indicates that the air tightness of the sealed chamber 31 and the air tightness of the exhaust pipeline 33 are normal, and thus it can be determined that the air removal device 30 is normal.

[0084] If the rate of change of the air pressure is greater than the preset rate of change, it indicates that the air tightness of the sealed chamber 31 or the air tightness of the exhaust pipeline 33 is abnormal, and thus it can be determined that the air removal device 30 is abnormal.

[0085] Please refer to Figure 7 In some embodiments, determining whether the air removal device 30 is normal according to the change of the air pressure (step S200) comprises:

[0086] S250, obtaining a first time when the air pressure is a first threshold value and obtaining a second time when the air pressure is a second threshold value when the negative pressure device 32 stops working, the second threshold value being greater than the first threshold value;

[0087] S260, calculating a time difference using the first time and the second time;

[0088] S270, comparing the time difference with a preset time difference to determine whether the air removal device 30 is normal.

[0089] Specifically, the first threshold value can be a lower limit value of the negative pressure condition of the air pressure in the sealed chamber 31, and the second threshold value can be an upper limit value of the negative pressure condition of the air pressure in the sealed chamber 31, both of which are negative values.

[0090] The time difference can be the difference between the first time and the second time, and the preset time difference is the time length for the air pressure in the sealed chamber 31 to change from the first threshold value to the second threshold value when the air removal device 30 is normal. The preset time difference can be a range, and when the time difference for the air pressure to change from the first threshold value to the second threshold value is within the range, it is determined that the air removal device 30 is normal, and when the time difference is outside the range, it is determined that the air removal device 30 is abnormal.

[0091] In this way, by comparing the time difference for the air pressure to change from the first threshold value to the second threshold value with the preset time difference, the normality of the air removal device 30 can be objectively and accurately determined, and the calculation steps can be simplified, making the calculation process simple and fast, thereby improving the determination efficiency.

[0092] In some embodiments, comparing the time difference for the air pressure to change from the first threshold value to the second threshold value with the preset time difference to determine whether the air removal device 30 is normal comprises:

[0093] If the time difference of the change of the air pressure from the first threshold value to the second threshold value is greater than or equal to the preset time difference, it is determined that the air removing device 30 is normal; if the time difference of the change of the air pressure from the first threshold value to the second threshold value is less than the preset time difference, it is determined that the air removing device 30 is abnormal.

[0094] Specifically, when the negative pressure device 32 stops working, if the time difference of the change of the air pressure from the first threshold value to the second threshold value is greater than or equal to the preset time difference, it indicates that the air pressure in the sealed chamber 31 is maintained at the negative pressure condition within the preset time difference, and the air tightness of the sealed chamber 31 and the air tightness of the exhaust pipeline 33 are normal, so it can be determined that the air removing device 30 is normal.

[0095] If the time difference of the change of the air pressure from the first threshold value to the second threshold value is less than the preset time difference, it indicates that the air pressure in the sealed chamber 31 does not reach the negative pressure condition within the preset time difference, and the air tightness of the sealed chamber 31 or the air tightness of the exhaust pipeline 33 is abnormal, so it can be determined that the air removing device 30 is abnormal.

[0096] In some embodiments, determining whether the air removing device 30 is normal according to the change of the air pressure comprises:

[0097] When the negative pressure device 32 stops working, the air pressure is recorded as the first threshold value;

[0098] When the air pressure is the second threshold value, the negative pressure device 32 is started;

[0099] If the air pressure is greater than the first threshold value within the preset time, it is determined that the air removing device 30 is abnormal, and if the air pressure is less than or equal to the first threshold value within the preset time, it is determined that the air removing device 30 is normal, and the second threshold value is greater than the first threshold value.

[0100] Specifically, when the negative pressure device 32 works, if the air pressure is greater than the first threshold value within the preset time, it indicates that the air pressure in the sealed chamber 31 does not reach the negative pressure condition within the preset time difference, and the air tightness of the sealed chamber 31, the air tightness of the exhaust pipeline 33 or the negative pressure device 32 is abnormal, so it can be determined that the air removing device 30 is abnormal.

[0101] If the air pressure is less than or equal to the first threshold value within the preset time, it indicates that the air pressure in the sealed chamber 31 reaches the negative pressure condition within the preset time difference, and the air tightness of the sealed chamber 31, the air tightness of the exhaust pipeline 33 and the negative pressure device 32 are normal, so it can be determined that the air removing device 30 is normal.

[0102] In some embodiments, determining whether the air removing device 30 is normal according to the change of the air pressure comprises:

[0103] When the negative pressure device 32 stops working, the air pressure is recorded as the first threshold value;

[0104] At a time interval of a preset time interval, it is determined whether the air pressure in the sealed chamber 31 is greater than a first threshold value;

[0105] If the air pressure in the sealed chamber 31 is greater than the first threshold value, a change rate of the air pressure is obtained.

[0106] If the change rate of the air pressure is greater than a preset change rate, it is determined that the degassing device 30 is abnormal; otherwise, it is determined that the degassing device 30 is normal.

[0107] Specifically, when the negative pressure device 32 stops working, the air pressure is a lower limit value of the negative pressure condition of the sealed chamber 31. With the gas in the liquid in the pipeline 20 entering the sealed chamber 31 from the pipeline 20 under the action of the negative pressure, the air pressure in the sealed chamber 31 rises.

[0108] If the change rate of the air pressure is greater than the preset change rate, it indicates that gas enters the sealed chamber 31 from the external environment, further indicating that the air tightness of the sealed chamber 31 or the air tightness of the exhaust pipeline 33 is abnormal, and thus it can be determined that the degassing device 30 is abnormal.

[0109] If the change rate of the air pressure is less than or equal to the preset change rate, it indicates that the air tightness of the sealed chamber 31 and the air tightness of the exhaust pipeline 33 are normal, and thus it can be determined that the degassing device 30 is normal.

[0110] In some embodiments, the method further comprises:

[0111] If the degassing device 30 is normal, the negative pressure device 32 is started.

[0112] If the degassing device 30 is abnormal, the negative pressure device 32 is not started.

[0113] Specifically, before the fluid system 100 is enabled, the negative pressure device 32 can be started to determine whether the degassing device 30 is normal. If the degassing device 30 is normal, the negative pressure device 32 is started during the operation of the fluid system 100. If the degassing device 30 is abnormal, the negative pressure device 32 is not started during the operation of the fluid system 100.

[0114] During the operation of the fluid system 100, the degassing device 30 can be determined to be normal every certain time. If the degassing device 30 is normal, the degassing device 30 is started when the air pressure in the sealed chamber 31 does not reach the negative pressure condition, and the negative pressure device 32 is closed when the air pressure in the sealed chamber 31 reaches the negative pressure condition. If the degassing device 30 is abnormal, the negative pressure device 32 is not started during the subsequent operation of the fluid system 100.

[0115] In one embodiment, the detector 34 can be connected to a computer device, and the computer determines whether the degassing device 30 is normal according to the change of the air pressure. When the degassing device 30 is abnormal, the computer can issue an alarm and close the working negative pressure device 32.

[0116] Thus, starting the degassing device 30 in the normal case can effectively remove the gas in the liquid in the pipeline 20, and closing the degassing device 30 in the abnormal case can reduce the waste of power resources and the consumption of the service life of the negative pressure device 32.

[0117] In some embodiments, the degassing device 30 comprises a first degassing device 37, and the first pipeline 21 is connected to the first degassing device 37.

[0118] In the normal case of the degassing device 30, the liquid in the pipeline 20 is controlled to be degassed by the degassing device 30, comprising:

[0119] The liquid in the first pipeline 21 is controlled to be degassed by the first degassing device 37, and the degassed liquid enters the reaction device 10.

[0120] Specifically, a negative pressure condition is formed in the sealed chamber 31 in the first degassing device 37, the gas in the liquid in the first pipeline 21 flows out of the first pipeline 21 to the sealed chamber 31 when passing through the sealed chamber 31, and the gas in the sealed chamber 31 is discharged to the atmosphere from the exhaust pipeline 33, and the liquid in the first pipeline 21 passing through the sealed chamber 31 enters the reaction device 10 from the liquid inlet 11.

[0121] Thus, the gas in the liquid in the first pipeline 21 can be removed, and the formation of bubbles in the reaction device 10 can be reduced or avoided.

[0122] In some embodiments, the degassing device 30 comprises a second degassing device 38, and the second pipeline 22 is connected to the second degassing device 38.

[0123] In the normal case of the degassing device 30, the liquid in the pipeline 20 is controlled to be degassed by the degassing device 30, comprising:

[0124] The liquid flowing out of the reaction device 10 and into the second pipeline 22 is controlled to be degassed by the second degassing device 38.

[0125] Specifically, the selection of components in the second degassing device 38 can be the same as or different from that of the first degassing device 37. A negative pressure condition is formed in the sealed chamber 31 in the second degassing device 38, the gas in the liquid in the second pipeline 22 flows out of the second pipeline 22 to the sealed chamber 31 when passing through the sealed chamber 31, and the gas in the sealed chamber 31 is discharged to the atmosphere from the exhaust pipeline 33, and the liquid in the second pipeline 22 passing through the sealed chamber 31 can be stored in the second pipeline 22 or the bypass pipeline 23 for repeated use.

[0126] Thus, the gas in the liquid in the second pipeline 22 can be removed, and the formation of bubbles in the reaction device 10 when the liquid is reused can be reduced or avoided.

[0127] In some embodiments, the degassing device 30 comprises a third degassing device 39, and the bypass pipeline 23 is connected to the third degassing device 39.

[0128] When the degassing device 30 is normal, the liquid in the control pipeline 20 is degassed by the degassing device 30, including:

[0129] The liquid flowing out of the reaction device 10 enters the bypass pipeline 23, and the liquid in the bypass pipeline 23 enters the third degassing device 39 for degassing.

[0130] Specifically, the components in the third degassing device 39 can be the same as or different from those in the first degassing device 37 or the second degassing device 38. The liquid degassed by the second degassing device 38 can enter the bypass pipeline 23, and a negative pressure condition is formed in the sealed chamber 31 in the third degassing device 39. The gas in the liquid in the bypass pipeline 23 flows from the bypass pipeline 23 to the sealed chamber 31 when passing through the sealed chamber 31, and the gas in the sealed chamber 31 is discharged from the exhaust pipeline 33 to the atmosphere. The liquid in the bypass pipeline 23 passing through the sealed chamber 31 can be stored in the bypass pipeline 23 for reuse.

[0131] Thus, the gas in the liquid in the bypass pipeline 23 can be removed, and the formation of bubbles in the reaction device 10 when the liquid is reused can be reduced or avoided.

[0132] In some embodiments, the method further comprises:

[0133] The liquid degassed in the bypass pipeline 23 enters the reaction device 10.

[0134] Specifically, the liquid degassed by the third degassing device 39 enters the first pipeline 21, and then enters the reaction device 10 from the liquid inlet 11. Thus, the reuse of the liquid can be realized, and the cost can be reduced.

[0135] The embodiment of the present application provides a sequencing system comprising the above fluid system 100.

[0136] The embodiment of the present application provides a computer storage medium, when a computer program is executed by a processor, the processor realizes the method for degassing the liquid in the fluid system 100 according to any one of the above embodiments.

[0137] Specifically, in an embodiment, the processor can be a central processing unit (CPU). The processor can also be other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, chips, or combinations thereof.

[0138] The computer program can be stored in the memory as a non-transitory computer readable storage medium, and used to store non-transitory software programs, non-transitory computer executable programs and modules, such as program instructions / modules corresponding to the methods in the method embodiments. The processor executes various functions and data processing of the processor by running the non-transitory software programs, instructions and modules stored in the memory, that is, implements the control method in the method embodiments.

[0139] The storage medium can include, but is not limited to, a U disk, a read-only memory (ROM), a random access memory (RAM), a mobile hard disk, a magnetic or optical disk, and various computer program storage media.

[0140] In the description of the present specification, the description of the terms "one embodiment", "certain embodiments", "illustrative embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiments or examples are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0141] Although the embodiments of the present application have been shown and described, those skilled in the art can understand that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and purposes of the present application, and the scope of the present application is defined by the claims and their equivalents.

Claims

1. A fluid system, characterized by, The fluid system comprises: a reaction device for providing a place for biochemical reaction; a pipeline in communication with the reaction device, the pipeline being used for delivering liquid to the reaction device and / or receiving liquid outflowing from the reaction device; a degassing device connected with the pipeline, the degassing device being used for removing gas in the liquid in the pipeline.

2. The fluid system according to claim 1, wherein: the reaction device comprises a liquid inlet and a liquid outlet; the pipeline comprises a first pipeline and a second pipeline, the first pipeline being in communication with the liquid inlet, the second pipeline being in communication with the liquid outlet, the first pipeline and / or the second pipeline being connected with the degassing device.

3. The fluid system according to claim 1 or 2, wherein: the reaction device comprises a liquid inlet and a liquid outlet, the pipeline comprises a bypass pipeline, one end of the bypass pipeline being in communication with the liquid inlet, the other end of the bypass pipeline being in communication with the liquid outlet, the bypass pipeline being connected with the degassing device.

4. The fluid system according to any one of claims 1-2, wherein: the degassing device comprises a sealed chamber, the pipeline passes through the sealed chamber, the part of the pipeline located in the sealed chamber is capable of allowing gas in the liquid in the pipeline to permeate out when the sealed chamber is under negative pressure, so that the liquid in the pipeline removes gas after passing through the sealed chamber.

5. The fluid system according to claim 4, wherein: the degassing device comprises a negative pressure device and an exhaust pipeline, one end of the exhaust pipeline being in communication with the sealed chamber, the negative pressure device being arranged on the exhaust pipeline and being used for generating negative pressure in the sealed chamber.

6. The fluid system according to claim 5, wherein: the degassing device comprises a detector between the negative pressure device and the sealed chamber, the detector being in communication with the exhaust pipeline, the detector being used for detecting the air pressure in the sealed chamber through the exhaust pipeline.

7. The fluid system according to claim 6, wherein: the degassing device comprises a multi-way valve arranged on the exhaust pipeline and between the detector and the negative pressure device, the multi-way valve being capable of making the negative pressure device communicate with the sealed chamber or the atmosphere.

8. The fluid system of claim 5, wherein, the negative pressure device is a pump.

9. The fluid system of claim 6, wherein, the detector is in communication with the exhaust pipeline through a three-way joint.

10. A sequencing system, comprising: The fluid system according to any one of claims 1-9.

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